##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib26_ATGAGC_L007_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2946028
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.778979018529355	27.0	22.0	33.0	2.0	33.0
2	24.79833491059827	27.0	15.0	33.0	2.0	33.0
3	25.682495549940462	33.0	22.0	33.0	6.0	33.0
4	27.779124977766674	33.0	22.0	37.0	2.0	37.0
5	27.81900511468323	33.0	15.0	37.0	6.0	37.0
6	28.85393451793398	33.0	22.0	37.0	15.0	37.0
7	28.52259754489774	33.0	27.0	37.0	6.0	37.0
8	28.26673235963813	33.0	22.0	37.0	6.0	37.0
9	29.002456867348172	33.0	27.0	37.0	15.0	37.0
10-11	28.600379052744916	33.0	24.5	37.0	10.5	37.0
12-13	28.871567412122353	33.0	27.0	37.0	10.5	37.0
14-15	29.14413729265302	33.0	22.0	37.0	6.0	40.0
16-17	29.94133490924051	33.0	27.0	37.0	15.0	40.0
18-19	30.213195699429875	33.0	27.0	37.0	15.0	40.0
20-21	30.172471035577395	33.0	27.0	37.0	15.0	40.0
22-23	30.142427023775742	33.0	27.0	37.0	15.0	40.0
24-25	29.692537884908084	33.0	27.0	37.0	10.5	40.0
26-27	29.118953044573914	33.0	22.0	37.0	6.0	40.0
28-29	29.233798864097693	33.0	22.0	37.0	15.0	40.0
30-31	29.081147226027724	33.0	22.0	37.0	10.5	40.0
32-33	28.84868915027284	33.0	22.0	37.0	6.0	40.0
34-35	28.851499035311274	33.0	22.0	37.0	10.5	40.0
36-37	29.069347949170883	33.0	24.5	37.0	6.0	40.0
38-39	29.03264700810719	33.0	22.0	37.0	10.5	40.0
40-41	29.0399225669274	33.0	22.0	37.0	10.5	40.0
42-43	28.880494177244753	33.0	22.0	37.0	15.0	40.0
44-45	28.557619275852097	33.0	22.0	37.0	10.5	40.0
46-47	28.142291926621198	33.0	22.0	37.0	6.0	40.0
48-49	27.925833019916986	33.0	22.0	37.0	6.0	40.0
50-51	27.096307468903895	30.0	22.0	35.0	6.0	38.5
52-53	26.813417591414613	30.0	22.0	37.0	6.0	37.0
54-55	27.697179897814955	33.0	22.0	37.0	6.0	40.0
56-57	27.570047704909797	33.0	22.0	37.0	6.0	40.0
58-59	27.732504239606683	33.0	22.0	37.0	6.0	40.0
60-61	27.484715522052063	33.0	22.0	37.0	6.0	40.0
62-63	27.31421459673839	33.0	22.0	37.0	6.0	40.0
64-65	27.259078664561233	33.0	22.0	37.0	6.0	38.5
66-67	27.122592521184455	30.0	22.0	37.0	6.0	37.0
68-69	26.819329619406197	27.0	22.0	37.0	6.0	37.0
70-71	26.703146915100604	27.0	22.0	35.0	6.0	37.0
72-73	26.473401644519335	27.0	22.0	33.0	6.0	37.0
74-75	26.395758458507522	27.0	22.0	33.0	6.0	37.0
76-77	26.017267147494863	27.0	22.0	33.0	6.0	37.0
78-79	25.462353039414424	27.0	15.0	33.0	6.0	37.0
80-81	25.32777726484609	27.0	15.0	33.0	6.0	37.0
82-83	24.91808326329553	27.0	15.0	33.0	6.0	37.0
84-85	25.007420669457318	27.0	15.0	33.0	6.0	37.0
86-87	24.709796885840866	27.0	15.0	33.0	6.0	37.0
88-89	24.358326024056797	27.0	15.0	33.0	6.0	37.0
90-91	24.205441530087292	27.0	15.0	33.0	6.0	37.0
92-93	24.084673669089362	27.0	15.0	33.0	6.0	37.0
94-95	23.870047908573852	27.0	15.0	33.0	6.0	37.0
96-97	23.73241343938347	27.0	15.0	33.0	6.0	37.0
98-99	23.483515940785356	27.0	15.0	33.0	6.0	37.0
100-101	22.997377485889476	27.0	15.0	33.0	6.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	8.343912027822313
2306	2	8.521685138324113
2306	3	7.542287613429753
2306	4	9.176547301238017
2306	5	8.946993026802168
2306	6	8.021447843337782
2306	7	8.230009854999093
2306	8	8.601596521970645
2306	9	7.5884412819720914
2306	10-11	7.8218341500511634
2306	12-13	7.4757807466764135
2306	14-15	7.982339868471364
2306	16-17	6.765180033977188
2306	18-19	6.217350518859234
2306	20-21	6.051331649371775
2306	22-23	5.67726340202892
2306	24-25	5.685662036671587
2306	26-27	6.229930020582607
2306	28-29	5.323412176478783
2306	30-31	5.180192721416148
2306	32-33	5.099948120189769
2306	34-35	4.367808315062714
2306	36-37	4.4206708494041855
2306	38-39	4.055925430101432
2306	40-41	3.361125404395139
2306	42-43	3.1112530831789584
2306	44-45	2.7177255102564573
2306	46-47	3.0102762091618978
2306	48-49	2.99830148661367
2306	50-51	2.842338737015467
2306	52-53	3.400485601662485
2306	54-55	2.910743123161051
2306	56-57	3.104230901146714
2306	58-59	2.6591589824985995
2306	60-61	2.6214099377334747
2306	62-63	2.1899082924942874
2306	64-65	1.8926177033647562
2306	66-67	1.6463887892925229
2306	68-69	1.74003903163495
2306	70-71	1.8721211833396048
2306	72-73	1.0592100396104769
2306	74-75	0.7662786460005933
2306	76-77	1.1195209188721194
2306	78-79	1.422462648564217
2306	80-81	1.2071530507920265
2306	82-83	1.041552424941635
2306	84-85	0.5621990900918519
2306	86-87	0.5617011648559398
2306	88-89	0.537616840778572
2306	90-91	0.5518319441258228
2306	92-93	0.47974667085630074
2306	94-95	0.6000290797387962
2306	96-97	0.3885933916901223
2306	98-99	0.11918360909607628
2306	100-101	0.09143455353067509
2307	1	8.316269906772852
2307	2	8.372326672599058
2307	3	7.457056465182497
2307	4	9.112891745907895
2307	5	9.031747590032431
2307	6	7.628517751612506
2307	7	8.236429141572703
2307	8	8.635255944624245
2307	9	7.527621359977136
2307	10-11	7.77581253732453
2307	12-13	7.283031721620638
2307	14-15	8.11551818971656
2307	16-17	6.777322914165282
2307	18-19	6.354088402754286
2307	20-21	6.34438619963494
2307	22-23	5.960540404407048
2307	24-25	6.125250347994019
2307	26-27	6.807583621486508
2307	28-29	5.940375452776024
2307	30-31	5.841633591399045
2307	32-33	5.77970516684136
2307	34-35	5.474304444436871
2307	36-37	5.394793066994662
2307	38-39	4.963528923531381
2307	40-41	4.380429520897035
2307	42-43	4.051137050645167
2307	44-45	4.0345405082681545
2307	46-47	4.417467561087239
2307	48-49	4.201695332622926
2307	50-51	3.904701454515589
2307	52-53	4.401669651716169
2307	54-55	4.0683927189452085
2307	56-57	4.399514662384174
2307	58-59	4.00274456393446
2307	60-61	3.8548386419216207
2307	62-63	3.536059735437391
2307	64-65	3.3805219471238814
2307	66-67	3.0480789697832975
2307	68-69	3.25433552491479
2307	70-71	2.9208574537829257
2307	72-73	2.314360341362921
2307	74-75	2.011966501915879
2307	76-77	2.175819614706487
2307	78-79	2.5385341454398294
2307	80-81	2.3228763032419657
2307	82-83	2.3701038397933054
2307	84-85	1.6943972997572345
2307	86-87	1.577373173725455
2307	88-89	1.8133107958416872
2307	90-91	1.7420388775302662
2307	92-93	1.614946273261804
2307	94-95	1.6457304824525174
2307	96-97	1.493481082268893
2307	98-99	1.2553333048775563
2307	100-101	0.9765987565505654
1101	1	7.977229264749791
1101	2	7.806808906409753
1101	3	7.123529895629858
1101	4	8.669554847109808
1101	5	8.556510127514699
1101	6	7.2574332432576085
1101	7	7.0515800582049515
1101	8	7.964520715010881
1101	9	7.285384197574064
1101	10-11	7.480057919962167
1101	12-13	7.223811746125726
1101	14-15	7.621547074256206
1101	16-17	6.7599269517762295
1101	18-19	5.901992260039933
1101	20-21	5.680230150272486
1101	22-23	5.105987853133179
1101	24-25	5.732661384518231
1101	26-27	6.270609324468758
1101	28-29	5.525877178649068
1101	30-31	5.447157325680436
1101	32-33	5.452824469686121
1101	34-35	5.289295641928565
1101	36-37	5.1203808770383255
1101	38-39	4.757950210980514
1101	40-41	4.284399242662531
1101	42-43	4.0356519037557135
1101	44-45	3.9969664094457826
1101	46-47	4.268545708037397
1101	48-49	4.005644790236218
1101	50-51	3.4505994172415164
1101	52-53	4.320239139375129
1101	54-55	4.1912815491222055
1101	56-57	4.430806446510246
1101	58-59	3.854093498099907
1101	60-61	4.077196927523044
1101	62-63	3.690455902178016
1101	64-65	3.44733815810363
1101	66-67	3.097175675960788
1101	68-69	3.232550706270402
1101	70-71	3.1954000354359167
1101	72-73	2.922553557061569
1101	74-75	2.218760550582335
1101	76-77	2.416864099223222
1101	78-79	2.9659187958148046
1101	80-81	2.400177973196211
1101	82-83	2.678570578795302
1101	84-85	2.0552846644170657
1101	86-87	2.1032523771476193
1101	88-89	2.1601179093286333
1101	90-91	1.9481324021503532
1101	92-93	1.7558902920450485
1101	94-95	1.9412449225847723
1101	96-97	1.8663391742065798
1101	98-99	1.6420303592222503
1101	100-101	1.2797303803418814
2201	1	6.947005802803769
2201	2	7.6979893797210615
2201	3	6.178401030644981
2201	4	8.299545897946402
2201	5	3.5869800253784803
2201	6	5.539298869268656
2201	7	6.422590556775756
2201	8	7.606995403571087
2201	9	6.381763051428141
2201	10-11	3.972861894696738
2201	12-13	6.22771205274217
2201	14-15	6.947175741854892
2201	16-17	5.90397249307447
2201	18-19	1.9029679488880618
2201	20-21	4.931670512928804
2201	22-23	5.000881004871211
2201	24-25	5.346824363179749
2201	26-27	6.023621207711116
2201	28-29	4.878187264935036
2201	30-31	5.0779353983007525
2201	32-33	5.1841671707009525
2201	34-35	4.8225473493933215
2201	36-37	4.929853225162265
2201	38-39	4.23967866842969
2201	40-41	3.939595063121235
2201	42-43	3.9056534694882856
2201	44-45	3.685894008784466
2201	46-47	4.136140757651088
2201	48-49	3.8339388392651657
2201	50-51	3.4848023999991646
2201	52-53	4.3127113420566126
2201	54-55	3.874791713769003
2201	56-57	3.9524808231112267
2201	58-59	3.66279340578847
2201	60-61	3.5302055086279935
2201	62-63	3.4318683042640785
2201	64-65	3.2953647785255313
2201	66-67	2.9888076516333086
2201	68-69	3.1820526771641013
2201	70-71	2.973119883582118
2201	72-73	2.442554640459459
2201	74-75	2.095381825845543
2201	76-77	2.4439392514183638
2201	78-79	2.769641603269765
2201	80-81	2.1925320780869377
2201	82-83	2.573665902968731
2201	84-85	2.107219791144935
2201	86-87	2.119749966958338
2201	88-89	2.1538960145992903
2201	90-91	1.9518569905333614
2201	92-93	1.9341628586294952
2201	94-95	2.028846212867137
2201	96-97	1.7488582523982288
2201	98-99	1.661506625387883
2201	100-101	1.2831057955207292
2304	1	1.4680252301893262
2304	2	4.801732534152773
2304	3	5.639593832930963
2304	4	4.119121735584422
2304	5	6.375885703872584
2304	6	6.148735187339007
2304	7	7.069835095324056
2304	8	6.282994021762644
2304	9	5.797401632607336
2304	10-11	6.519977157986649
2304	12-13	6.461442912463987
2304	14-15	6.653174388257092
2304	16-17	5.40547708191977
2304	18-19	5.108528849505049
2304	20-21	4.765757679446978
2304	22-23	4.510842085728555
2304	24-25	4.6168719981279125
2304	26-27	5.547548927691274
2304	28-29	4.901304153414273
2304	30-31	4.784262057509544
2304	32-33	4.986035510104706
2304	34-35	4.580146782923666
2304	36-37	4.709471928867966
2304	38-39	4.2771117194514545
2304	40-41	3.657018345567703
2304	42-43	3.1586086943296507
2304	44-45	3.0379354204654163
2304	46-47	3.9849306365966655
2304	48-49	3.58728526233552
2304	50-51	3.156560478115967
2304	52-53	3.949314666860076
2304	54-55	3.6741887369822557
2304	56-57	4.312430222198948
2304	58-59	3.6874153319186505
2304	60-61	3.62209910424847
2304	62-63	3.3466281560607634
2304	64-65	3.2022771996190897
2304	66-67	3.072144992159842
2304	68-69	3.1871132492553684
2304	70-71	3.1770630642753694
2304	72-73	2.925868623858264
2304	74-75	2.259256312347773
2304	76-77	2.384266181962232
2304	78-79	2.9311355284931047
2304	80-81	2.653485410507269
2304	82-83	2.7950331290780355
2304	84-85	2.435534579829291
2304	86-87	2.2806255341845514
2304	88-89	2.4489330833804246
2304	90-91	2.3642051852319135
2304	92-93	2.1568308196516277
2304	94-95	2.3933642610088555
2304	96-97	2.2248845298401285
2304	98-99	2.0531895324071527
2304	100-101	1.7105104897199546
1102	1	6.80986372128848
1102	2	7.253751651480098
1102	3	5.962722233670149
1102	4	6.043831640995176
1102	5	6.387116937538341
1102	6	5.537881886427723
1102	7	6.548169413333991
1102	8	6.17961191370334
1102	9	4.605599161566669
1102	10-11	5.772348499691507
1102	12-13	5.640549393452105
1102	14-15	5.88872760979131
1102	16-17	5.087286937853349
1102	18-19	4.993190784348442
1102	20-21	5.265020951750699
1102	22-23	5.014524743528703
1102	24-25	1.3089631345057313
1102	26-27	5.137246228442457
1102	28-29	4.575249744928861
1102	30-31	4.42985570054492
1102	32-33	4.54070100663705
1102	34-35	4.546870688684994
1102	36-37	4.406489644547552
1102	38-39	3.4449628311190104
1102	40-41	3.4868687895003063
1102	42-43	3.5492335706094487
1102	44-45	-4.0266318328738215
1102	46-47	0.750255524303217
1102	48-49	-1.1952319480753033
1102	50-51	2.0467291741753186
1102	52-53	-0.6216245264722211
1102	54-55	2.0902479261070717
1102	56-57	-1.2507222713356398
1102	58-59	2.3664810419925217
1102	60-61	3.4086510915045523
1102	62-63	2.9670786940951785
1102	64-65	2.5587610208122804
1102	66-67	2.4602669520026197
1102	68-69	2.7785046405158695
1102	70-71	2.666593111658255
1102	72-73	2.220025031327978
1102	74-75	2.019954000940224
1102	76-77	2.3583626676292866
1102	78-79	-1.6517102609810728
1102	80-81	1.1225262664865312
1102	82-83	2.1473025997956547
1102	84-85	1.9012782402154542
1102	86-87	1.9137046313563282
1102	88-89	1.826290759110691
1102	90-91	1.7632097284011756
1102	92-93	1.6197404747198831
1102	94-95	2.0107791910888224
1102	96-97	1.9381308673044515
1102	98-99	1.8750527817120854
1102	100-101	1.5043495923283423
2202	1	-20.509109533064418
2202	2	-7.567094936120432
2202	3	-0.6835469073485854
2202	4	1.6189623267603643
2202	5	1.2414977138872523
2202	6	1.6514691112900834
2202	7	3.9197102698060533
2202	8	2.7065649153931233
2202	9	3.1622792170072387
2202	10-11	2.0578818390240805
2202	12-13	1.6245272638847723
2202	14-15	2.73379665339386
2202	16-17	1.7017539913279727
2202	18-19	1.6439663166974299
2202	20-21	1.1442030390757907
2202	22-23	1.4095310689149514
2202	24-25	2.4099355941977585
2202	26-27	3.491249076651755
2202	28-29	-1.063100938579474
2202	30-31	0.784208742324843
2202	32-33	1.473070795811477
2202	34-35	1.7720061714593278
2202	36-37	3.1048949544438678
2202	38-39	2.630227133229173
2202	40-41	2.0426363419115
2202	42-43	0.7879056656335592
2202	44-45	0.7946397602141033
2202	46-47	-1.2203273230204807
2202	48-49	0.8014072292292944
2202	50-51	1.7340949352904573
2202	52-53	1.57434490265274
2202	54-55	-0.6886367942641947
2202	56-57	1.0662385007947606
2202	58-59	1.9129064488574556
2202	60-61	2.0792229164201466
2202	62-63	-2.1388914039923534
2202	64-65	-0.08712316915023521
2202	66-67	0.4850967712589309
2202	68-69	1.4235805403895512
2202	70-71	-2.4302186270397286
2202	72-73	-4.453774359416151
2202	74-75	-1.4064077425904813
2202	76-77	-0.16913407445160544
2202	78-79	1.7390939201063773
2202	80-81	1.7038317624068107
2202	82-83	-0.5879596311492641
2202	84-85	-0.28182990746291026
2202	86-87	0.7157224819438959
2202	88-89	1.2973043849973038
2202	90-91	1.4943805152582748
2202	92-93	1.4527777742632288
2202	94-95	1.848370852053833
2202	96-97	1.4505686906859552
2202	98-99	1.310766550485294
2202	100-101	1.2034463619295401
2305	1	5.696833941582298
2305	2	6.978521019569477
2305	3	6.560106778948491
2305	4	7.706816299256182
2305	5	7.690349611552463
2305	6	6.019212440853003
2305	7	2.880210764810805
2305	8	5.964275523929256
2305	9	6.383581619709592
2305	10-11	4.685953018281946
2305	12-13	5.137197290850953
2305	14-15	5.574510683967297
2305	16-17	5.053573933073963
2305	18-19	4.355262236932205
2305	20-21	5.104352124805317
2305	22-23	5.086170593004816
2305	24-25	5.302760726327648
2305	26-27	6.1329215863589255
2305	28-29	5.270617153458339
2305	30-31	5.383882744763483
2305	32-33	5.446116464134036
2305	34-35	5.318824084678052
2305	36-37	4.693010485686493
2305	38-39	4.513794353551486
2305	40-41	4.0125284220559685
2305	42-43	3.8187337677919544
2305	44-45	3.0457915557914745
2305	46-47	3.7131333996203075
2305	48-49	3.416495833977372
2305	50-51	2.419317940994585
2305	52-53	3.827963729149719
2305	54-55	4.193606922262347
2305	56-57	4.601430968343834
2305	58-59	4.508226559279898
2305	60-61	4.465196532336147
2305	62-63	3.7855051642686917
2305	64-65	4.101117546478907
2305	66-67	3.7015748069389645
2305	68-69	3.9931604037285524
2305	70-71	3.646921358981075
2305	72-73	-0.8664680341594462
2305	74-75	2.1950864220862414
2305	76-77	3.1640085332201977
2305	78-79	3.818362481682101
2305	80-81	3.3701828497635766
2305	82-83	3.5091784365903145
2305	84-85	2.9630254607478648
2305	86-87	2.907890713001972
2305	88-89	2.882490403152623
2305	90-91	2.722722128069748
2305	92-93	2.8623021507667055
2305	94-95	2.9891010336731725
2305	96-97	2.952913624108369
2305	98-99	2.9048816716126247
2305	100-101	2.5330103829115664
1103	1	-20.509109533064418
1103	2	-7.606954868056878
1103	3	-0.9202218508000044
1103	4	2.82297764252635
1103	5	4.5511504798952735
1103	6	4.612116557597034
1103	7	3.853343514409282
1103	8	4.075837166289563
1103	9	4.2698629041174065
1103	10-11	4.8330746202759265
1103	12-13	4.583744990435363
1103	14-15	4.095038258097045
1103	16-17	2.913416023073374
1103	18-19	3.170982742029551
1103	20-21	2.465364988033791
1103	22-23	2.646766073498064
1103	24-25	2.999059720165185
1103	26-27	4.03032541171336
1103	28-29	3.701598735724943
1103	30-31	3.53280033030439
1103	32-33	2.9328774662275237
1103	34-35	2.3540653168470946
1103	36-37	2.700557623075536
1103	38-39	2.4064670724102477
1103	40-41	2.5584577756562688
1103	42-43	2.2925959332567665
1103	44-45	2.5955431349098568
1103	46-47	1.9882560341130038
1103	48-49	-1.0282633482865862
1103	50-51	-2.0273602452554655
1103	52-53	1.4872895330348754
1103	54-55	2.1981687177147577
1103	56-57	3.192480727136477
1103	58-59	2.7021163971070266
1103	60-61	3.1258723678045897
1103	62-63	3.0187180087879497
1103	64-65	2.873143328071059
1103	66-67	2.618062467954452
1103	68-69	2.822753042736828
1103	70-71	2.855529449448401
1103	72-73	2.3263773819634537
1103	74-75	2.011071599168595
1103	76-77	2.2875126000852433
1103	78-79	2.8646017870593106
1103	80-81	2.382935647002551
1103	82-83	2.547346544399499
1103	84-85	2.0764713029924238
1103	86-87	2.0570522029318
1103	88-89	1.963470588660556
1103	90-91	-2.3617673676617734
1103	92-93	-3.0037857707810325
1103	94-95	-3.1466693081412416
1103	96-97	0.261309715095436
1103	98-99	-1.0913451732069355
1103	100-101	0.7052643369791589
2203	1	2.5124063593561203
2203	2	3.266076478924351
2203	3	-5.581017285381311
2203	4	-3.199034599170126
2203	5	-0.0387453364890078
2203	6	0.1506489270620932
2203	7	2.3823640003039763
2203	8	-7.545811219277926
2203	9	-6.8181996849749105
2203	10-11	-12.055326931802094
2203	12-13	-9.934976085813336
2203	14-15	-1.338020538912751
2203	16-17	-1.2615080136484735
2203	18-19	-0.5158731377525143
2203	20-21	-1.3479424647192388
2203	22-23	-3.177744293779856
2203	24-25	-0.8649898239470346
2203	26-27	-1.2233860451328482
2203	28-29	0.6594408867087473
2203	30-31	-2.0596023688583287
2203	32-33	-0.029565510635638503
2203	34-35	0.14929842856568243
2203	36-37	-0.07393228371437388
2203	38-39	0.6905029996217245
2203	40-41	-0.5370238890976893
2203	42-43	0.31037777310060477
2203	44-45	0.17542417757848838
2203	46-47	0.6102045969326504
2203	48-49	0.593972120847809
2203	50-51	0.5436760554883051
2203	52-53	1.427004178149467
2203	54-55	0.46203741489791383
2203	56-57	0.6281499796989038
2203	58-59	0.9781687067636753
2203	60-61	0.534243302127237
2203	62-63	0.9851771334898949
2203	64-65	0.5967030529653385
2203	66-67	-0.13977627745274646
2203	68-69	0.6320003454390708
2203	70-71	0.9119160137459552
2203	72-73	0.2965497473850647
2203	74-75	-0.09676636241541914
2203	76-77	0.19472961153500634
2203	78-79	0.8992855306204746
2203	80-81	0.6581597798913954
2203	82-83	0.15814968207074642
2203	84-85	0.22607393166510903
2203	86-87	0.24410839008066887
2203	88-89	0.49296863910308986
2203	90-91	0.07619383795558932
2203	92-93	0.3390807625095569
2203	94-95	0.7315190818697133
2203	96-97	0.47441650911265043
2203	98-99	0.34461114784055624
2203	100-101	-0.16314231805809243
2310	1	-20.509109533064418
2310	2	-20.28062278757932
2310	3	-21.202777676579355
2310	4	-23.142310882788706
2310	5	-23.31796294040099
2310	6	-24.356930535040597
2310	7	-23.903455070600668
2310	8	-23.511825889204577
2310	9	-24.438737582285423
2310	10-11	-24.139067763097938
2310	12-13	-24.417249924444143
2310	14-15	-24.66198141910835
2310	16-17	-25.645615593110577
2310	18-19	-19.762989408288618
2310	20-21	-19.871985409553453
2310	22-23	-26.104394660528023
2310	24-25	-25.69848615646537
2310	26-27	-18.64905682134667
2310	28-29	-19.049319987105154
2310	30-31	-19.508267995840143
2310	32-33	-17.84159411260519
2310	34-35	-10.11947742229405
2310	36-37	-14.56715950803502
2310	38-39	-19.260603427146307
2310	40-41	-11.581126103945506
2310	42-43	-4.939837110612906
2310	44-45	-13.550214690148868
2310	46-47	-18.602272518699717
2310	48-49	-18.967045380957018
2310	50-51	-10.972475820930129
2310	52-53	-14.451339033519465
2310	54-55	-18.22077497915594
2310	56-57	-17.74892546791733
2310	58-59	-10.38515767317865
2310	60-61	-7.439084194612114
2310	62-63	-4.778084332625287
2310	64-65	-14.948346544788304
2310	66-67	-10.508879888296848
2310	68-69	-5.522228326729152
2310	70-71	-3.328597294087178
2310	72-73	-4.177979901581697
2310	74-75	-3.9911230474427626
2310	76-77	-3.0316759572935936
2310	78-79	-3.5257697109467117
2310	80-81	-2.9232095528622715
2310	82-83	-12.156298979168819
2310	84-85	-10.063971517985294
2310	86-87	-6.3259872755503
2310	88-89	-4.496537557126125
2310	90-91	-1.9644057225050098
2310	92-93	-1.5410991814112727
2310	94-95	-2.062961747112766
2310	96-97	-1.2033930331202285
2310	98-99	-1.3557293755383917
2310	100-101	-1.1336511985887299
2204	1	-20.509109533064418
2204	2	-20.28062278757932
2204	3	-21.202777676579355
2204	4	-23.142310882788706
2204	5	-23.31796294040099
2204	6	-24.356930535040597
2204	7	-23.903455070600668
2204	8	-23.511825889204577
2204	9	-24.438737582285423
2204	10-11	-18.133936400207954
2204	12-13	-18.661502798007362
2204	14-15	-18.632219514346446
2204	16-17	-19.85107536322552
2204	18-19	-26.0513254604909
2204	20-21	-13.717802771205884
2204	22-23	-6.460305990577282
2204	24-25	-15.524635581752728
2204	26-27	-24.75101440699104
2204	28-29	-12.571895305132461
2204	30-31	-15.21623334432414
2204	32-33	-18.647934635538782
2204	34-35	-18.83758395106183
2204	36-37	-25.077355266599458
2204	38-39	-12.808764909211146
2204	40-41	-7.025508157267481
2204	42-43	-4.759302872954294
2204	44-45	-13.590749276037002
2204	46-47	-19.469001063073534
2204	48-49	-17.836460650017894
2204	50-51	-9.923130023658643
2204	52-53	-2.5621493502208246
2204	54-55	-24.209967475077637
2204	56-57	-18.05707209665377
2204	58-59	-10.87252363829677
2204	60-61	-14.8462539056084
2204	62-63	-11.4728089232547
2204	64-65	-5.163541004188758
2204	66-67	-3.1880433070535616
2204	68-69	-6.569156607520007
2204	70-71	-6.521888451468172
2204	72-73	-3.101447088985431
2204	74-75	-2.825774295577723
2204	76-77	-2.8587260916298263
2204	78-79	-16.63701129651608
2204	80-81	-6.823924963625775
2204	82-83	-3.3407226727054145
2204	84-85	-2.1739765539199
2204	86-87	-1.829401933870173
2204	88-89	-2.725226245291946
2204	90-91	-2.1148780422761426
2204	92-93	-1.5591585652593203
2204	94-95	-1.07143778758239
2204	96-97	-1.1486955508196637
2204	98-99	-1.6259203258567538
2204	100-101	-1.9728672669702938
2311	1	8.277837771599422
2311	2	8.476494111269865
2311	3	7.463636617787696
2311	4	9.083007106308813
2311	5	8.933248446637172
2311	6	7.977412287491205
2311	7	8.125163948175828
2311	8	8.197289798257568
2311	9	7.5810988804644275
2311	10-11	7.701861976453845
2311	12-13	7.298755526797528
2311	14-15	7.907749047275658
2311	16-17	6.721207544381855
2311	18-19	6.102899251804672
2311	20-21	5.992214867504401
2311	22-23	5.546574449102501
2311	24-25	5.521229167580664
2311	26-27	6.189551916449297
2311	28-29	5.165066246755785
2311	30-31	4.721006886257115
2311	32-33	4.856537451338951
2311	34-35	4.508709632266783
2311	36-37	4.52311414587783
2311	38-39	3.9020094734339956
2311	40-41	2.987818467686889
2311	42-43	2.8991583372989354
2311	44-45	2.6493029041115683
2311	46-47	2.8207310393791794
2311	48-49	2.677928425021463
2311	50-51	2.484550075679074
2311	52-53	2.984019116121413
2311	54-55	2.518076134855736
2311	56-57	2.8333241054787877
2311	58-59	2.2525612731715263
2311	60-61	2.344429652845996
2311	62-63	1.9889647542398592
2311	64-65	1.8809169249759279
2311	66-67	1.312852676770742
2311	68-69	1.452572896119623
2311	70-71	1.2128486188648786
2311	72-73	0.657331240116001
2311	74-75	0.36004432059657887
2311	76-77	0.712702604120075
2311	78-79	1.0035652958661068
2311	80-81	0.8414468963903978
2311	82-83	0.8549763811595383
2311	84-85	0.21242209585100014
2311	86-87	0.1269274639471405
2311	88-89	0.0367717576102109
2311	90-91	-0.23180076115378867
2311	92-93	-0.2982395471790191
2311	94-95	-0.053337099240053476
2311	96-97	-0.08272008588275526
2311	98-99	-0.3229768443224508
2311	100-101	-0.4870773192946558
2205	1	8.532247837031022
2205	2	8.625906526161025
2205	3	7.656334579139475
2205	4	9.400182678792874
2205	5	9.215744073687787
2205	6	7.976049316497047
2205	7	8.293333294798511
2205	8	8.685871429383514
2205	9	7.760474658284185
2205	10-11	8.017120693196564
2205	12-13	7.745528442469961
2205	14-15	8.251062059152517
2205	16-17	7.156308357805955
2205	18-19	6.533282932176874
2205	20-21	6.450083451188142
2205	22-23	6.187909535805865
2205	24-25	6.0830620937997395
2205	26-27	6.647940296841718
2205	28-29	5.876457609002806
2205	30-31	5.713803379474243
2205	32-33	5.7675762501333665
2205	34-35	5.511613836803821
2205	36-37	5.492785621144822
2205	38-39	4.789316711999152
2205	40-41	4.377989135897781
2205	42-43	4.068144248432752
2205	44-45	4.195208354903627
2205	46-47	4.386159118159892
2205	48-49	3.9049894002183123
2205	50-51	3.8014060097183986
2205	52-53	4.428796379005156
2205	54-55	4.076201287231104
2205	56-57	4.22383797096051
2205	58-59	3.780406865092651
2205	60-61	3.727596146717353
2205	62-63	3.4397276220614934
2205	64-65	3.287409914080328
2205	66-67	2.977781446075099
2205	68-69	2.8832589529924206
2205	70-71	2.7533341288792847
2205	72-73	2.257575490317251
2205	74-75	1.8608891235837746
2205	76-77	2.1632986103049205
2205	78-79	2.44877203133219
2205	80-81	2.2390644475919608
2205	82-83	2.197621178017311
2205	84-85	1.5587744486276058
2205	86-87	1.5720473850243835
2205	88-89	1.5245656705968003
2205	90-91	1.2423320721788222
2205	92-93	0.9654532577490365
2205	94-95	1.3367696940426512
2205	96-97	1.1638099995410442
2205	98-99	0.8465951585613176
2205	100-101	0.5439932052326277
2206	1	8.11519300878307
2206	2	8.319811186382243
2206	3	7.315356235385927
2206	4	8.88837727592177
2206	5	8.794715298904656
2206	6	7.620285831977384
2206	7	8.117158692573543
2206	8	8.650449374279614
2206	9	7.499111146790835
2206	10-11	7.817922317497224
2206	12-13	7.555859189009048
2206	14-15	8.241459374444041
2206	16-17	7.003407965475908
2206	18-19	6.403417254946177
2206	20-21	6.1895882492081
2206	22-23	5.792614018951205
2206	24-25	6.129939138016958
2206	26-27	6.751310453517327
2206	28-29	5.827984519812034
2206	30-31	5.6671655482940295
2206	32-33	5.705736709861938
2206	34-35	5.33191377726823
2206	36-37	5.409160542451996
2206	38-39	4.740147127331024
2206	40-41	4.094737267580097
2206	42-43	3.8741196881945044
2206	44-45	3.761636333699979
2206	46-47	4.186116124148107
2206	48-49	3.864231565427829
2206	50-51	3.9120420745540514
2206	52-53	4.432647772296654
2206	54-55	3.925726883260861
2206	56-57	4.087682855159137
2206	58-59	3.973703572471244
2206	60-61	3.9232614341444503
2206	62-63	3.415541782271113
2206	64-65	3.3285328375547927
2206	66-67	2.9667899850507204
2206	68-69	3.0880201236166798
2206	70-71	2.9236988691218215
2206	72-73	2.1493631128129707
2206	74-75	1.982235618737107
2206	76-77	2.1015434802089956
2206	78-79	2.595065008790659
2206	80-81	2.1198290638419373
2206	82-83	2.3216193971152244
2206	84-85	1.7468726984090317
2206	86-87	1.726171267041547
2206	88-89	1.7443817071307421
2206	90-91	1.4402196247702364
2206	92-93	1.3428383206746517
2206	94-95	1.5376905538796812
2206	96-97	1.2165904261103933
2206	98-99	1.2608683838978365
2206	100-101	1.0581630674329574
2309	1	8.017180149475266
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2309	3	7.378240841939164
2309	4	8.706234090756269
2309	5	8.308690498752448
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2309	7	7.125480114584516
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2309	9	7.4149330526352095
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2309	12-13	6.168811583492364
2309	14-15	7.274526517399586
2309	16-17	6.49939102064603
2309	18-19	6.135017132101694
2309	20-21	6.063099436420458
2309	22-23	5.549375180741819
2309	24-25	5.832763843534629
2309	26-27	6.428962444860808
2309	28-29	5.596518875673965
2309	30-31	5.504076019842984
2309	32-33	5.616045477579263
2309	34-35	5.120310365668697
2309	36-37	5.404208886839694
2309	38-39	4.266261317754736
2309	40-41	3.9426916466004265
2309	42-43	4.0545626397258765
2309	44-45	3.9137520695203776
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2309	56-57	4.290489365670624
2309	58-59	3.8058938493879637
2309	60-61	3.985043987111908
2309	62-63	3.54077493461795
2309	64-65	3.0232605603067704
2309	66-67	2.8754801147672744
2309	68-69	3.257275042726299
2309	70-71	3.0380601208726397
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2309	74-75	2.312125380576262
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2207	1	6.943637719682833
2207	2	7.176689131271061
2207	3	6.34701099628624
2207	4	7.234020478158037
2207	5	6.946280508457846
2207	6	6.644591020327113
2207	7	6.951828107759436
2207	8	6.716238354244279
2207	9	5.604204091425483
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2207	12-13	6.048092425513595
2207	14-15	6.373859662886581
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2207	20-21	4.697516016990889
2207	22-23	4.745224950630053
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2207	26-27	5.492180859281149
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2207	34-35	2.291019055127382
2207	36-37	3.6250961281596297
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2207	66-67	2.3692472361786265
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2207	76-77	1.6443306217717115
2207	78-79	2.0583050140522197
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2207	82-83	1.5195033744569777
2207	84-85	1.1377715344196986
2207	86-87	1.3251814358988696
2207	88-89	1.4337359684448039
2207	90-91	1.2248813362763364
2207	92-93	1.338043657804974
2207	94-95	1.378836208978079
2207	96-97	1.1149846019188416
2207	98-99	1.0602331316494897
2207	100-101	0.6891137670266545
1113	1	5.7642671934001015
1113	2	6.485054304022523
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1113	4	7.101378591731056
1113	5	7.1108352815390035
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1113	12-13	3.259047900607456
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1113	30-31	4.096074027946226
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2104	12-13	-4.665137673388017
2104	14-15	-12.359175142705212
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2104	30-31	-3.310389796574782
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2104	98-99	-2.1022101467192336
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2110	3	-21.202777676579355
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2110	5	-11.738465841754953
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2110	26-27	-18.909621757087752
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2110	30-31	-19.318754520873195
2110	32-33	-19.015671642967646
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2110	36-37	-25.077355266599458
2110	38-39	-14.79663480405096
2110	40-41	-17.611518930548982
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2110	66-67	-2.5270665972874156
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2110	86-87	-1.8035185057306933
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2110	96-97	-1.4749918700543532
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2109	1	7.8631038427317606
2109	2	7.909663836624503
2109	3	6.978153852083064
2109	4	8.725125422943776
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2109	78-79	1.987692424473746
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2211	1	6.409907597927102
2211	2	6.022024729292113
2211	3	5.447334799627601
2211	4	7.392902822004508
2211	5	7.017216156328551
2211	6	6.820781871950231
2211	7	6.761019752033004
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2211	36-37	4.2817899142276765
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2211	70-71	2.449279522377452
2211	72-73	1.962640339280263
2211	74-75	1.5189039403543703
2211	76-77	1.7992513369796725
2211	78-79	2.128267964701209
2211	80-81	2.0496490520262753
2211	82-83	2.152784586037601
2211	84-85	1.5995595221450998
2211	86-87	1.6140471617313352
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2211	90-91	1.5989621784312398
2211	92-93	1.4877885659509893
2211	94-95	1.5421135282485778
2211	96-97	1.3639663335126855
2211	98-99	1.2829787768624215
2211	100-101	1.0858672059287215
2210	1	7.273464566796335
2210	2	7.414622885784517
2210	3	6.83621178035321
2210	4	8.332723929226411
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2210	9	7.127007394838113
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2210	30-31	5.3247865276773965
2210	32-33	5.3490600823115955
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2210	38-39	4.0562422132471205
2210	40-41	4.052114470362692
2210	42-43	4.16533107417456
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2210	46-47	4.3913635999301555
2210	48-49	4.173161977285638
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2210	58-59	3.920547635609033
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2210	64-65	3.4520573427345695
2210	66-67	3.2084636669453026
2210	68-69	3.532594715303901
2210	70-71	3.4517008820585353
2210	72-73	3.0375639917369597
2210	74-75	2.692297533621776
2210	76-77	2.7906444829652237
2210	78-79	3.2593729412436936
2210	80-81	3.01302300502822
2210	82-83	3.1149674626327304
2210	84-85	2.5149720480955544
2210	86-87	2.470015897851667
2210	88-89	2.7251781065120895
2210	90-91	2.6026971747048684
2210	92-93	2.3229921884865483
2210	94-95	2.6382101627469154
2210	96-97	2.453299611265667
2210	98-99	2.3800757028133823
2210	100-101	2.0542482958541655
2209	1	5.042353735898352
2209	2	2.6268030535514804
2209	3	4.449422203970116
2209	4	1.7002135050273388
2209	5	4.876542335330647
2209	6	5.309404324605033
2209	7	6.3617251006117534
2209	8	6.937905347108387
2209	9	6.336496341664411
2209	10-11	6.247852404132804
2209	12-13	5.610820949535551
2209	14-15	4.845683323077594
2209	16-17	4.3806635229555155
2209	18-19	3.6444738626227746
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2209	22-23	3.802234843752288
2209	24-25	4.136174404952108
2209	26-27	1.3224476674664558
2209	28-29	-0.032954972534334104
2209	30-31	2.834876378536908
2209	32-33	4.084542112634725
2209	34-35	4.217336894166703
2209	36-37	3.869190622311553
2209	38-39	-1.1729162498259278
2209	40-41	-0.5379155332558305
2209	42-43	-4.68528289686401
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2209	46-47	2.2917491983675156
2209	48-49	1.6764641732989531
2209	50-51	2.85052516085058
2209	52-53	3.66193537727408
2209	54-55	2.9942929270724754
2209	56-57	3.4574785402032227
2209	58-59	2.603987203293329
2209	60-61	2.800855638436449
2209	62-63	2.612687407744218
2209	64-65	1.9204623178042937
2209	66-67	2.0170459588422034
2209	68-69	-0.7928552211550866
2209	70-71	1.5747910428906167
2209	72-73	1.9941577919448825
2209	74-75	1.644404058051414
2209	76-77	2.12699541865112
2209	78-79	2.537547228521621
2209	80-81	2.049770814121711
2209	82-83	2.064155405376791
2209	84-85	1.437244137958217
2209	86-87	1.4569469123403778
2209	88-89	1.6348915151446946
2209	90-91	1.6480895236692739
2209	92-93	1.3664588818403445
2209	94-95	1.586227837242916
2209	96-97	1.364595159204864
2209	98-99	1.2941254602431123
2209	100-101	1.1320492140969272
2208	1	4.56139626943175
2208	2	6.09752479288926
2208	3	4.545853810173437
2208	4	2.703539785045759
2208	5	3.7580169150840135
2208	6	4.725837146150557
2208	7	3.2743421704766256
2208	8	3.6725402154593176
2208	9	3.8822608849797398
2208	10-11	4.341554089321594
2208	12-13	4.889078080810947
2208	14-15	5.453521098956024
2208	16-17	3.7588074417043984
2208	18-19	1.805144870141902
2208	20-21	4.026737242952947
2208	22-23	2.9777161343044725
2208	24-25	3.6462697007713274
2208	26-27	5.006594526663438
2208	28-29	4.209468591573504
2208	30-31	4.303733735853747
2208	32-33	3.2931190720220265
2208	34-35	3.5904644312970895
2208	36-37	3.492384168478271
2208	38-39	3.6253816041317
2208	40-41	3.1345796684746254
2208	42-43	2.990838562552735
2208	44-45	-1.4044836120658317
2208	46-47	2.4909342172609
2208	48-49	2.675898350421825
2208	50-51	3.08446861805713
2208	52-53	1.664844526390251
2208	54-55	0.6433279048216427
2208	56-57	-1.197988422562883
2208	58-59	1.7669345580953575
2208	60-61	2.8599750183887345
2208	62-63	2.440088871412044
2208	64-65	2.817569018703388
2208	66-67	2.900716349841069
2208	68-69	2.038719404191049
2208	70-71	-0.1418143459140495
2208	72-73	-0.22746212582248404
2208	74-75	-1.9334801532278973
2208	76-77	0.44598414670090847
2208	78-79	1.7510493534742295
2208	80-81	1.917596726028318
2208	82-83	2.1764233825787507
2208	84-85	1.5661788246922086
2208	86-87	1.862235268189437
2208	88-89	2.2610664204118613
2208	90-91	2.1437883543731964
2208	92-93	1.950278827933726
2208	94-95	1.8851270850690227
2208	96-97	1.9257797490667556
2208	98-99	2.1839452299636655
2208	100-101	1.8590022740326084
1307	1	7.214965801630385
1307	2	7.115338183602891
1307	3	6.813106144614199
1307	4	8.397058302598175
1307	5	4.958188636635544
1307	6	5.937147068771523
1307	7	3.4940942827008996
1307	8	5.4536835276322755
1307	9	5.63568946559295
1307	10-11	6.723764095309139
1307	12-13	5.503444425453747
1307	14-15	5.371941884726461
1307	16-17	4.074148418688832
1307	18-19	4.943115202091356
1307	20-21	5.072615322042537
1307	22-23	4.673345298627872
1307	24-25	5.360057070219447
1307	26-27	6.148123328429882
1307	28-29	5.4683683931263545
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1212	38-39	-25.303017782774365
1212	40-41	-19.858144560996806
1212	42-43	-16.170476082540915
1212	44-45	-13.141769974805555
1212	46-47	-16.427291677429388
1212	48-49	-19.077262440471955
1212	50-51	-11.291834060073112
1212	52-53	-4.479966581347579
1212	54-55	-3.317004145047907
1212	56-57	-3.5492541169277914
1212	58-59	-2.194591366997898
1212	60-61	-13.09810892634948
1212	62-63	-10.770388303251803
1212	64-65	-15.087058981148177
1212	66-67	-11.322119055530731
1212	68-69	-6.765560361256902
1212	70-71	-14.03180128848022
1212	72-73	-10.625690312128214
1212	74-75	-7.522259418795528
1212	76-77	-17.620695135424512
1212	78-79	-9.049440240616875
1212	80-81	-6.641214676407781
1212	82-83	-4.477692530194904
1212	84-85	-3.8425259069511597
1212	86-87	-2.8931347832178353
1212	88-89	-2.8393327026112125
1212	90-91	-3.2820453346741196
1212	92-93	-3.0083258299916693
1212	94-95	-2.827303713461305
1212	96-97	-12.173304733202846
1212	98-99	-9.126212442293227
1212	100-101	-5.792792944086429
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	507.0
3	1057.0
4	637.0
5	3328.0
6	3994.0
7	4610.0
8	9177.0
9	16871.0
10	23369.0
11	30511.0
12	39781.0
13	48316.0
14	54485.0
15	59075.0
16	63631.0
17	69197.0
18	76085.0
19	82641.0
20	88027.0
21	95073.0
22	102482.0
23	107982.0
24	111313.0
25	113046.0
26	116291.0
27	118610.0
28	119662.0
29	120026.0
30	122854.0
31	127691.0
32	134932.0
33	148936.0
34	162570.0
35	173901.0
36	177183.0
37	160330.0
38	57671.0
39	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06007454540028	22.27719234315387	11.686397270752474	23.97633584069337
2	25.16006867376458	25.06143011439026	28.49249052079636	21.286010691048798
3	20.679832112959122	24.317935426501354	28.47725913177751	26.524973328762012
4	25.715016781387757	24.096338860891137	25.72836670238154	24.460277655339567
5	24.100326624870124	26.42729099086159	25.33937461876666	24.133007765501624
6	23.581352061212268	26.127061113717247	25.368931784845365	24.922655040225113
7	25.74634821675256	25.640225773901765	25.63756051269115	22.975865496654535
8	23.294668451083762	25.830530110531374	26.664865671371864	24.209935767013
9	23.606831072732007	25.05645825818116	26.498734574829335	24.837976094257503
10-11	24.804280642422246	25.232544034818694	25.27016531274724	24.69301001001182
12-13	24.666136451448033	25.26522708097526	25.19408170857852	24.874554758998187
14-15	24.40164047655423	25.81496427124034	25.31546605653478	24.46792919567065
16-17	24.848958238914076	25.770862065839918	25.004444968156598	24.375734727089412
18-19	24.956915918714408	25.685711914320024	24.725027079671495	24.632345087294073
20-21	24.59698446173951	25.842988604062388	25.13178368367156	24.428243250526542
22-23	24.795332224776406	25.4770824794019	25.150065772782483	24.57751952303921
24-25	24.653920975165974	25.494406139642273	25.21248092083385	24.639191964357902
26-27	24.394651439110465	25.744701980882795	25.386883966731098	24.47376261327564
28-29	24.889874991927332	25.61297884736458	25.058632609158877	24.43851355154921
30-31	24.86956790419195	25.408515962569428	25.00284680113289	24.719069332105736
32-33	24.475089925460956	25.728087805398765	25.25292438211518	24.543897887025096
34-35	24.893314071461912	25.581370787792995	25.03978647578392	24.48552866496117
36-37	24.774662014904077	25.532137249169807	25.041128886850483	24.65207184907563
38-39	24.522128993040532	25.75342635451915	25.335262877444414	24.38918177499591
40-41	24.960095146766427	25.576942290835564	24.94050752037817	24.522455042019836
42-43	24.796728405806004	25.5009607279007	25.04359964096421	24.65871122532909
44-45	24.486055127516508	25.649170900252884	25.334858501822843	24.529915470407765
46-47	24.96925849617398	25.61717429361642	24.921186991100576	24.492380219109027
48-49	24.8246860471252	25.456759902172415	25.04739178867757	24.67116226202481
50-51	24.668374475129873	25.687819255233013	25.15310202359961	24.4907042460375
52-53	24.843902310050993	25.573111955475802	25.070951914118787	24.512033820354414
54-55	24.776627847891238	25.418070952287124	25.04936171977891	24.755939480042723
56-57	24.51185624081171	25.714677306789714	25.27533323262871	24.498133219769862
58-59	24.988743141590938	25.552965156489293	24.909427972310542	24.548863729609227
60-61	24.868412628807963	25.34554162160525	24.94692447649016	24.83912127309663
62-63	24.623224115978694	25.61568902515755	25.190298137305607	24.570788721558152
64-65	24.99847512023355	25.514941158540932	24.901088996106786	24.585494725118732
66-67	24.987904165148464	25.393863422451407	24.92026988858179	24.697962523818344
68-69	24.76998929068769	25.611423038365345	25.093749251545667	24.524838419401306
70-71	25.1643417348124	25.470209010733726	24.768563913455484	24.596885340998387
72-73	24.95937264259833	25.43195284967343	24.840399501073797	24.768275006654445
74-75	24.8209154084617	25.6025808705273	24.962596202431424	24.61390751857957
76-77	25.230580742772023	25.53668619451455	24.667830233377703	24.564902829335725
78-79	25.197607698186015	25.342687526336704	24.698192691969652	24.76151208350763
80-81	25.086777599464018	25.532751139275156	24.79783100258256	24.58264025867827
82-83	25.294380415722102	25.43811457353835	24.709175840585566	24.558329170153986
84-85	25.389755805450044	25.2850883822387	24.547881907022447	24.777273905288805
86-87	25.172636698857755	25.564145319437564	24.739675223679775	24.52354275802491
88-89	25.51380284340901	25.43850426066321	24.510274697994593	24.537418197933185
90-91	25.570121050074572	25.267275031341203	24.334020744646516	24.828583173937712
92-93	25.406719722325693	25.456243702183563	24.572462525070133	24.564574050420614
94-95	25.788915653448413	25.351889362141765	24.264898557697308	24.59429642671251
96-97	25.78914393061339	25.28122387321367	24.250734020031302	24.67889817614164
98-99	25.695344915636078	25.4718440738565	24.32733634380136	24.505474666706068
100-101	26.42934489925125	25.205013470248254	23.777020868933924	24.58862076156657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	205.0
1	207.5
2	211.0
3	243.5
4	282.5
5	326.5
6	380.0
7	458.0
8	617.5
9	833.5
10	1091.0
11	1398.5
12	1851.0
13	2399.0
14	3016.0
15	3752.0
16	4715.5
17	5835.0
18	7051.0
19	8678.0
20	10586.0
21	12593.5
22	15171.0
23	18325.0
24	21738.0
25	25779.0
26	30072.0
27	34796.0
28	39987.0
29	45213.5
30	50672.5
31	55777.5
32	61030.5
33	66357.5
34	70413.0
35	73925.5
36	76968.0
37	78982.5
38	79851.5
39	79742.0
40	78514.0
41	76072.5
42	72951.0
43	69123.5
44	65333.5
45	61770.0
46	58237.0
47	54881.0
48	51670.5
49	48305.0
50	44963.0
51	42365.0
52	40266.0
53	38725.0
54	37834.5
55	37578.0
56	37818.0
57	38448.5
58	39708.0
59	41712.0
60	44333.0
61	46783.5
62	49631.5
63	52837.0
64	56035.5
65	58975.5
66	61163.5
67	62536.0
68	62388.5
69	60652.0
70	57483.0
71	52951.0
72	47279.0
73	40651.0
74	33955.0
75	27640.0
76	21927.5
77	17256.0
78	13519.0
79	10568.0
80	8340.5
81	6615.0
82	5215.5
83	4228.5
84	3407.0
85	2686.0
86	2157.5
87	1721.5
88	1376.5
89	1047.5
90	785.5
91	618.5
92	472.0
93	356.5
94	270.0
95	193.0
96	120.5
97	74.0
98	45.0
99	18.5
100	5.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	13.556965514244942
2	10.871587099647389
3	9.607749824509474
4	11.516217768466559
5	9.01495165694284
6	5.281925358482676
7	9.576419504498938
8	9.90961389369008
9	5.337423812672521
10-11	6.255643191442852
12-13	5.294111257598367
14-15	7.239764863063081
16-17	2.634598177614062
18-19	3.074139145995897
20-21	3.0106299057578543
22-23	3.0672994282471175
24-25	3.438595967180217
26-27	4.29656473054567
28-29	1.7126449578890628
30-31	2.226794857346909
32-33	3.0142449426821467
34-35	1.9725542323426664
36-37	3.046916051035496
38-39	1.3573360470436806
40-41	1.1362756905229687
42-43	0.5416784904963564
44-45	1.2876320252217563
46-47	1.3557406786357766
48-49	1.2570315013978144
50-51	1.5420593422737325
52-53	2.428558044933721
54-55	1.8023589728271423
56-57	1.8017140366622448
58-59	1.54696425152782
60-61	1.3826752495224077
62-63	0.5995360532893781
64-65	1.220711412111494
66-67	0.5533043134688468
68-69	0.792185274545931
70-71	0.992522813768233
72-73	0.08508065775342256
74-75	0.10675390729483901
76-77	0.4541199201093812
78-79	0.8795401808808334
80-81	0.29307257093279493
82-83	0.494716954489231
84-85	0.06588532084555883
86-87	0.09930319738984152
88-89	0.08192386494629379
90-91	0.08886541472110925
92-93	0.17068744764136662
94-95	0.44984297501585185
96-97	0.3673420619220184
98-99	0.06678483707554714
100-101	0.22358918516728285
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2946028.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.87577155839926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.27344972878613	98.1573893719769
2	0.5316636264251096	1.0513730254463851
3	0.08450575276810213	0.250667145182083
4	0.0507723344948786	0.20080614988009002
5	0.03558889571286219	0.17594397612603285
6	0.008485047824223728	0.05033793901980228
7	0.010924147834484104	0.07560934820268425
8	0.0031905305272532615	0.02523729340502341
9	0.0014199356269450475	0.012635750760970585
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.0366405207282483E-4	0.0	0.0
2	2.3760806075162898E-4	0.0	0.0
3	3.054960781092373E-4	0.0	0.0
4	3.054960781092373E-4	0.0	3.394400867880414E-5
5	3.054960781092373E-4	0.0	3.394400867880414E-5
6	3.733840954668455E-4	0.0	3.394400867880414E-5
7	4.412721128244538E-4	0.0	3.394400867880414E-5
8	5.770481475396704E-4	0.0	3.394400867880414E-5
9	5.770481475396704E-4	3.394400867880414E-5	3.394400867880414E-5
10-11	6.449361648972786E-4	3.394400867880414E-5	5.091601301820621E-5
12-13	7.976842039518973E-4	3.394400867880414E-5	6.788801735760828E-5
14-15	9.67404247345918E-4	6.788801735760828E-5	6.788801735760828E-5
16-17	0.0010183202603641242	6.788801735760828E-5	6.788801735760828E-5
18-19	0.0010352922647035262	6.788801735760828E-5	6.788801735760828E-5
20-21	0.0010692362733823305	6.788801735760828E-5	6.788801735760828E-5
22-23	0.0011031802820611345	6.788801735760828E-5	6.788801735760828E-5
24-25	0.001188040303758145	6.788801735760828E-5	6.788801735760828E-5
26-27	0.0013068443341339594	6.788801735760828E-5	6.788801735760828E-5
28-29	0.0015783964035643925	6.788801735760828E-5	6.788801735760828E-5
30-31	0.0018838924816736297	6.788801735760828E-5	6.788801735760828E-5
32-33	0.002410024616195094	6.788801735760828E-5	6.788801735760828E-5
34-35	0.0033095408461834034	6.788801735760828E-5	6.788801735760828E-5
36-37	0.00451455315428095	6.788801735760828E-5	6.788801735760828E-5
38-39	0.006364501627275776	6.788801735760828E-5	6.788801735760828E-5
40-41	0.008723610230452664	6.788801735760828E-5	6.788801735760828E-5
42-43	0.011931319050599656	6.788801735760828E-5	6.788801735760828E-5
44-45	0.016479816213559408	6.788801735760828E-5	1.0183202603641242E-4
46-47	0.023251645944980838	6.788801735760828E-5	1.0183202603641242E-4
48-49	0.031007851928087582	6.788801735760828E-5	1.0183202603641242E-4
50-51	0.04186993470530491	6.788801735760828E-5	1.0183202603641242E-4
52-53	0.055396622163808355	6.788801735760828E-5	1.0183202603641242E-4
54-55	0.07199524240774358	6.788801735760828E-5	1.0183202603641242E-4
56-57	0.09217495556729263	6.788801735760828E-5	1.0183202603641242E-4
58-59	0.11892283440619031	6.788801735760828E-5	1.0183202603641242E-4
60-61	0.14852200997410753	6.788801735760828E-5	1.0183202603641242E-4
62-63	0.18314489882648774	6.788801735760828E-5	1.3577603471521656E-4
64-65	0.22555793767065352	6.788801735760828E-5	1.3577603471521656E-4
66-67	0.27202728555193634	8.486002169701034E-5	1.6972004339402068E-4
68-69	0.324861135060495	1.0183202603641242E-4	2.0366405207282483E-4
70-71	0.3878442431640161	1.3577603471521656E-4	2.0366405207282483E-4
72-73	0.452677299740532	1.6972004339402068E-4	2.0366405207282483E-4
74-75	0.5273032028208828	1.6972004339402068E-4	2.3760806075162898E-4
76-77	0.6095834798583041	1.8669204773342276E-4	2.3760806075162898E-4
78-79	0.7030143637467126	2.0366405207282483E-4	2.3760806075162898E-4
80-81	0.810294403176073	2.0366405207282483E-4	2.3760806075162898E-4
82-83	0.926416856866262	2.0366405207282483E-4	2.3760806075162898E-4
84-85	1.0522303250342495	2.0366405207282483E-4	2.3760806075162898E-4
86-87	1.1874632556106053	2.0366405207282483E-4	2.3760806075162898E-4
88-89	1.3357137135152821	2.0366405207282483E-4	2.3760806075162898E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCCGA	740	2.737109E-4	8.361905	1
GTTTAAG	1155	1.1368844E-5	7.3055596	1
GTGTAGG	2190	0.0	6.218796	92-93
GTAGGGA	1630	0.0	5.98813	94-95
TCGTGTA	2170	0.0	5.847831	90-91
TGTAGGG	2090	0.0	5.538894	92-93
GTCATGT	1235	0.009530003	5.4658604	1
CGTGTAG	2125	0.0	5.331846	90-91
TCGGAAG	5860	0.0	5.306807	94-95
>>END_MODULE