FastQCFastQC Report
Thu 11 Sep 2014
NYCLib26_ATGAGC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib26_ATGAGC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946028
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA7402.737109E-48.3619051
GTTTAAG11551.1368844E-57.30555961
GTGTAGG21900.06.21879692-93
GTAGGGA16300.05.9881394-95
TCGTGTA21700.05.84783190-91
TGTAGGG20900.05.53889492-93
GTCATGT12350.0095300035.46586041
CGTGTAG21250.05.33184690-91
TCGGAAG58600.05.30680794-95
Produced by FastQC (version 0.11.2)