##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib26_ATGAGC_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2946028 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.238004526772997 33.0 33.0 33.0 27.0 33.0 2 31.62258165910168 33.0 33.0 33.0 27.0 33.0 3 31.802928892732858 33.0 33.0 33.0 27.0 33.0 4 35.63217932755561 37.0 37.0 37.0 33.0 37.0 5 35.14524335817582 37.0 37.0 37.0 33.0 37.0 6 34.92427804487941 37.0 33.0 37.0 33.0 37.0 7 34.715459934528795 37.0 33.0 37.0 27.0 37.0 8 34.75321823146284 37.0 33.0 37.0 27.0 37.0 9 34.66453238054764 37.0 33.0 37.0 27.0 37.0 10-11 34.51251753208048 37.0 33.0 37.0 27.0 37.0 12-13 34.51133865665906 37.0 33.0 37.0 27.0 37.0 14-15 35.412440920452894 37.0 33.0 40.0 27.0 40.0 16-17 35.296448302595905 37.0 33.0 40.0 27.0 40.0 18-19 35.49880483145442 37.0 33.0 40.0 27.0 40.0 20-21 35.13844114855664 37.0 33.0 40.0 27.0 40.0 22-23 35.14437473099373 37.0 33.0 40.0 27.0 40.0 24-25 34.95804147143204 37.0 33.0 40.0 27.0 40.0 26-27 34.50913959405681 37.0 33.0 40.0 27.0 40.0 28-29 34.09412639662624 37.0 33.0 38.5 22.0 40.0 30-31 33.94004605523098 37.0 33.0 37.0 22.0 40.0 32-33 33.87744227142444 37.0 33.0 37.0 22.0 40.0 34-35 33.67198292073259 37.0 33.0 37.0 22.0 40.0 36-37 33.29336805352834 37.0 33.0 37.0 22.0 40.0 38-39 33.11937581720201 37.0 33.0 37.0 22.0 40.0 40-41 32.75962092009988 37.0 33.0 37.0 22.0 40.0 42-43 32.30640798390239 37.0 33.0 37.0 22.0 40.0 44-45 32.10620452351437 37.0 27.0 37.0 18.5 40.0 46-47 31.91716015597951 35.0 27.0 37.0 18.5 40.0 48-49 31.917585644128295 33.0 27.0 37.0 22.0 40.0 50-51 32.235444639358484 37.0 30.0 37.0 22.0 40.0 52-53 32.08724594606704 33.0 27.0 37.0 22.0 40.0 54-55 31.704569678224374 33.0 27.0 37.0 18.5 40.0 56-57 31.72820353370708 33.0 27.0 37.0 22.0 40.0 58-59 31.440975781628687 33.0 27.0 37.0 15.0 40.0 60-61 31.348740405725948 33.0 27.0 37.0 15.0 40.0 62-63 31.327617049125127 33.0 27.0 37.0 15.0 40.0 64-65 30.9869344418994 33.0 27.0 37.0 15.0 40.0 66-67 30.638163147125553 33.0 27.0 37.0 15.0 40.0 68-69 30.44820313995658 33.0 27.0 37.0 15.0 37.0 70-71 30.299233747948087 33.0 27.0 37.0 15.0 37.0 72-73 30.09876365737189 33.0 27.0 37.0 15.0 37.0 74-75 29.75155497503758 33.0 27.0 37.0 15.0 37.0 76-77 28.65548545363452 33.0 24.5 33.0 15.0 37.0 78-79 29.619730192652618 33.0 27.0 37.0 15.0 37.0 80-81 29.350272298837623 33.0 27.0 37.0 15.0 37.0 82-83 29.331465790549174 33.0 27.0 37.0 15.0 37.0 84-85 29.216165291029142 33.0 27.0 37.0 15.0 37.0 86-87 29.008766209961344 33.0 27.0 37.0 15.0 37.0 88-89 28.877133041505374 33.0 27.0 33.0 15.0 37.0 90-91 28.783671098849027 33.0 27.0 33.0 15.0 37.0 92-93 28.56132070027848 33.0 27.0 33.0 15.0 37.0 94-95 28.456066948447194 33.0 27.0 33.0 15.0 37.0 96-97 28.314780103922978 33.0 27.0 33.0 15.0 37.0 98-99 28.018204681014574 33.0 22.0 33.0 15.0 37.0 100-101 27.18338997456915 33.0 22.0 33.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1448675694296107 2306 2 0.5892506262353265 2306 3 0.5138386348223705 2306 4 0.3771730824931865 2306 5 0.6582286561897064 2306 6 0.7603378357913044 2306 7 0.9523085478581166 2306 8 0.7459372724320303 2306 9 1.0244279416183062 2306 10-11 1.2276113542853224 2306 12-13 1.3824131036479557 2306 14-15 1.5555765143809808 2306 16-17 1.5081903316012273 2306 18-19 1.2855456092853643 2306 20-21 1.5674423647698532 2306 22-23 1.1433368188194066 2306 24-25 1.2046596698392378 2306 26-27 1.390916337450065 2306 28-29 1.590626419690416 2306 30-31 1.4321764294449508 2306 32-33 1.1741587341044166 2306 34-35 1.070850502147593 2306 36-37 1.2468205258050773 2306 38-39 1.0070146487967193 2306 40-41 1.1339333855023028 2306 42-43 1.1666503766134078 2306 44-45 0.9658685168549752 2306 46-47 0.8539301900700096 2306 48-49 0.9193398166608091 2306 50-51 1.0067222272991678 2306 52-53 1.1079793258190875 2306 54-55 1.5869348073135967 2306 56-57 1.1072025654713258 2306 58-59 1.0379435690156633 2306 60-61 0.8171720983339839 2306 62-63 0.5358498605103748 2306 64-65 0.5856035808979243 2306 66-67 0.8248571179757995 2306 68-69 0.6702350793841028 2306 70-71 0.7466450652487282 2306 72-73 0.34010209362868693 2306 74-75 0.5629069927436774 2306 76-77 0.6406001173687912 2306 78-79 0.6328225459981454 2306 80-81 1.030150966863939 2306 82-83 0.9560533248415446 2306 84-85 0.6698501382402071 2306 86-87 0.49566005896108223 2306 88-89 0.38804311802516267 2306 90-91 0.07951093834331502 2306 92-93 -0.19042641413032513 2306 94-95 -0.16489994605803204 2306 96-97 -0.05740677390043558 2306 98-99 0.07053078704616667 2306 100-101 -0.03813165934515439 2307 1 0.7031045716898987 2307 2 0.46749648261845067 2307 3 0.34284316180984575 2307 4 0.32455417655087615 2307 5 0.5470370952891912 2307 6 0.6439838328121468 2307 7 0.7762853642540435 2307 8 0.7698128750946935 2307 9 1.0318281475429458 2307 10-11 1.1436420991031255 2307 12-13 1.3374849280510404 2307 14-15 1.3434822715232357 2307 16-17 1.3962804126316044 2307 18-19 1.3038832750441784 2307 20-21 1.4496804759574715 2307 22-23 1.1143231378834315 2307 24-25 1.1888560061406181 2307 26-27 1.2669440897130997 2307 28-29 1.4521789355342563 2307 30-31 1.583266721018525 2307 32-33 1.1426562981752895 2307 34-35 1.1428376473715005 2307 36-37 1.268219691985614 2307 38-39 0.8756822154472275 2307 40-41 1.1022387950558468 2307 42-43 1.1230490959064063 2307 44-45 1.0057226062894031 2307 46-47 0.9640202361286541 2307 48-49 0.9996947232983757 2307 50-51 0.8777422019132963 2307 52-53 1.1848532849524176 2307 54-55 1.378447262931914 2307 56-57 1.1312487504082682 2307 58-59 1.0224373908250435 2307 60-61 0.8483538134536985 2307 62-63 0.7608402302144306 2307 64-65 0.8920196055013037 2307 66-67 0.9458616261469501 2307 68-69 0.7011741661645345 2307 70-71 0.6927604530860627 2307 72-73 0.5882184780656345 2307 74-75 0.690750020749153 2307 76-77 0.8213748725986676 2307 78-79 0.9388558295933613 2307 80-81 1.4219512836337955 2307 82-83 1.1143283137311286 2307 84-85 0.9084560976056117 2307 86-87 0.8763434006231705 2307 88-89 0.7461437661285153 2307 90-91 0.4596985698169931 2307 92-93 0.5595111261483545 2307 94-95 0.5565602020976606 2307 96-97 0.44468193239836395 2307 98-99 0.4078110726816675 2307 100-101 0.5547572194175601 1101 1 1.040889007281958 1101 2 0.5613976568277437 1101 3 0.3517332249365239 1101 4 0.28031043556607216 1101 5 0.46201161974426697 1101 6 0.6000552549708686 1101 7 0.7500110526153279 1101 8 0.6925115471514545 1101 9 0.9006563237777954 1101 10-11 0.9899302963887564 1101 12-13 1.213297140067084 1101 14-15 1.2889827319555565 1101 16-17 1.2693727821095138 1101 18-19 0.9810154785441441 1101 20-21 1.2875304066574955 1101 22-23 0.902258186572972 1101 24-25 0.9582418313234058 1101 26-27 1.0405702528711558 1101 28-29 1.2265086153349998 1101 30-31 1.228826776868921 1101 32-33 0.7671665170366779 1101 34-35 0.9860510801958355 1101 36-37 1.1014184439874484 1101 38-39 0.9075018080608146 1101 40-41 0.9958522525049744 1101 42-43 1.049374452688017 1101 44-45 0.7342574396830202 1101 46-47 0.6986007981040387 1101 48-49 0.6890019201505027 1101 50-51 0.7188274712426868 1101 52-53 0.9583759042779221 1101 54-55 1.3458616511640002 1101 56-57 1.0615230517866507 1101 58-59 0.9041141510915658 1101 60-61 0.7642199606056437 1101 62-63 0.46259525970005555 1101 64-65 0.5867366469692854 1101 66-67 0.7277142655741535 1101 68-69 0.5720373078947496 1101 70-71 0.6721034157393149 1101 72-73 0.5252645973949868 1101 74-75 0.6345862556183732 1101 76-77 0.6418307219701802 1101 78-79 0.6788662438285442 1101 80-81 1.2824212238588224 1101 82-83 1.1025357741882402 1101 84-85 0.8532897220758144 1101 86-87 0.7249404287185222 1101 88-89 0.7222799480715345 1101 90-91 0.4316733328247402 1101 92-93 0.5678357595766848 1101 94-95 0.5843017409590878 1101 96-97 0.45833826092730234 1101 98-99 0.5576034292486653 1101 100-101 0.49789481130095226 2201 1 1.4781901740866488 2201 2 0.7766861820115523 2201 3 0.30772493471648943 2201 4 0.19034396153645616 2201 5 0.3513493519698585 2201 6 0.3542595066397496 2201 7 0.5431089917847629 2201 8 0.6031245425833731 2201 9 0.5797899319676887 2201 10-11 0.9091443580029264 2201 12-13 1.0377316212085432 2201 14-15 1.0686542175166807 2201 16-17 1.0692573557108176 2201 18-19 0.8666830520000062 2201 20-21 1.1659422937585262 2201 22-23 0.9347310348408726 2201 24-25 0.8884318637659803 2201 26-27 1.065887480678569 2201 28-29 1.3497782211265772 2201 30-31 1.521381644293676 2201 32-33 1.0484474421100671 2201 34-35 0.9197842689771818 2201 36-37 0.9637214288228435 2201 38-39 0.6571635801435818 2201 40-41 0.9428322066757602 2201 42-43 1.0214172958802656 2201 44-45 0.9704555070792722 2201 46-47 0.8913854423488203 2201 48-49 1.0731093635392455 2201 50-51 0.8380339268100165 2201 52-53 1.054760063813454 2201 54-55 1.4000745106517485 2201 56-57 1.0760178396039208 2201 58-59 1.1805202679857523 2201 60-61 0.8700054166567952 2201 62-63 0.7704813203762733 2201 64-65 0.8764444040981871 2201 66-67 0.8323813359178409 2201 68-69 0.8634116797465872 2201 70-71 0.8155182116894863 2201 72-73 0.8187187592754093 2201 74-75 0.7578001332033182 2201 76-77 0.8443746292669374 2201 78-79 0.7894966775142045 2201 80-81 1.3873065320929427 2201 82-83 1.1903735568734675 2201 84-85 1.0790032434751495 2201 86-87 1.0218275891178017 2201 88-89 0.9633487794562328 2201 90-91 0.8008699190825048 2201 92-93 0.9130245824722927 2201 94-95 0.8385618321426058 2201 96-97 0.5545969021385666 2201 98-99 0.7678431718452003 2201 100-101 0.8807699637929183 2304 1 -1.9683267479376063 2304 2 -0.3847357419643629 2304 3 -0.21763405850218476 2304 4 -0.07984684241036888 2304 5 0.43926204157742177 2304 6 0.5654777626169434 2304 7 0.5603203674432891 2304 8 0.7286156000669095 2304 9 0.8774569376749639 2304 10-11 0.9042641096056769 2304 12-13 1.1778851588997483 2304 14-15 1.0909425146147314 2304 16-17 1.1026319214593059 2304 18-19 0.8090861253697241 2304 20-21 1.137533281536136 2304 22-23 0.9918155216046998 2304 24-25 1.1170070046011844 2304 26-27 1.2045559846272837 2304 28-29 1.4931146884055693 2304 30-31 1.5695458945907816 2304 32-33 1.0859478780926892 2304 34-35 1.0207330633313632 2304 36-37 1.2497486923548493 2304 38-39 0.9667354759857929 2304 40-41 1.1343615836976255 2304 42-43 1.074933008437437 2304 44-45 1.1276893371250338 2304 46-47 1.098872584708431 2304 48-49 1.0157704680935211 2304 50-51 0.9594613377554388 2304 52-53 1.168982990190358 2304 54-55 1.6086318621137536 2304 56-57 1.2354850547367278 2304 58-59 0.9792962083104975 2304 60-61 0.7211387983585524 2304 62-63 0.5082219058445112 2304 64-65 0.5433582664989984 2304 66-67 0.6376772680974092 2304 68-69 0.7384228998875493 2304 70-71 0.6829793028052364 2304 72-73 0.5602903496733305 2304 74-75 0.7112260974558176 2304 76-77 0.7557049968926144 2304 78-79 0.7291525153984288 2304 80-81 1.286159782394666 2304 82-83 1.1073434558639832 2304 84-85 0.8544811381304633 2304 86-87 0.7466751747229914 2304 88-89 0.5792563939696329 2304 90-91 0.4682821480872512 2304 92-93 0.46232472778273603 2304 94-95 0.18245020853819938 2304 96-97 0.28371083414272036 2304 98-99 0.746090203701165 2304 100-101 0.6997562952248515 1102 1 1.4615009332939195 1102 2 0.08941153935843005 1102 3 0.22284033405841086 1102 4 0.13880337554422084 1102 5 0.23604266122412554 1102 6 0.13331998277464407 1102 7 -5.313268804557378 1102 8 -1.3458587856652358 1102 9 0.16349898885039238 1102 10-11 0.6250221929070605 1102 12-13 0.8394681738204142 1102 14-15 0.8253787220632418 1102 16-17 0.4932438491146769 1102 18-19 0.7145681225684655 1102 20-21 1.0709767163147674 1102 22-23 0.5644389259909275 1102 24-25 0.89777858425564 1102 26-27 0.9490482064085697 1102 28-29 0.7629219249758847 1102 30-31 1.1222701144606688 1102 32-33 0.8772369007610266 1102 34-35 1.0505193852398946 1102 36-37 1.2337662033989574 1102 38-39 0.8851741973198415 1102 40-41 1.007984879773062 1102 42-43 1.1200692980670404 1102 44-45 0.9956731349634893 1102 46-47 0.7664849186563956 1102 48-49 0.6915129582000858 1102 50-51 0.6504049318039655 1102 52-53 0.8122201470254709 1102 54-55 1.2895287764619923 1102 56-57 1.0289119261725403 1102 58-59 0.8270952799467928 1102 60-61 0.6616267388487813 1102 62-63 0.5111980736197452 1102 64-65 0.34838325534647296 1102 66-67 0.5763172056069479 1102 68-69 0.7181458988456306 1102 70-71 0.492614619417445 1102 72-73 0.33720015191641295 1102 74-75 0.5386448696269319 1102 76-77 0.448010684531404 1102 78-79 0.6291948735634101 1102 80-81 1.2094455388859977 1102 82-83 1.1392096755434125 1102 84-85 0.798015819123215 1102 86-87 0.5195281166323049 1102 88-89 0.5151280318039078 1102 90-91 0.05294096291146033 1102 92-93 0.3198797797766382 1102 94-95 0.2923047945777597 1102 96-97 0.04237658754090745 1102 98-99 0.2216629596791293 1102 100-101 0.461058392190111 2202 1 1.5260191588338188 2202 2 0.9099475712058123 2202 3 0.44427717994514637 2202 4 -0.2010611512040441 2202 5 0.10405229999862087 2202 6 0.46452608657001804 2202 7 0.6327613906511331 2202 8 -4.125385507743907 2202 9 -0.3685069418241369 2202 10-11 0.5489248443318573 2202 12-13 0.02809270346354964 2202 14-15 0.08881433524956606 2202 16-17 0.2898256051485504 2202 18-19 0.8787490338525998 2202 20-21 1.1948951871568099 2202 22-23 -2.864799058989078 2202 24-25 -2.9816957278199183 2202 26-27 -1.382135400247158 2202 28-29 0.5935385799059532 2202 30-31 1.2864560846254207 2202 32-33 1.2070508601290513 2202 34-35 -2.138725184617172 2202 36-37 -0.23060654063787922 2202 38-39 0.3737957035378656 2202 40-41 1.3061692296676313 2202 42-43 1.3652388407966107 2202 44-45 1.3332395015254548 2202 46-47 0.6469489263278021 2202 48-49 0.9025143451666011 2202 50-51 -3.9679410927729073 2202 52-53 -0.38293450474023416 2202 54-55 1.3339724710017862 2202 56-57 1.4119866733356332 2202 58-59 1.4123068863758839 2202 60-61 1.41594523740158 2202 62-63 1.368411287463509 2202 64-65 1.2858529913371868 2202 66-67 1.5072656873952148 2202 68-69 1.316748005149261 2202 70-71 1.2238192710165023 2202 72-73 1.1175304623007634 2202 74-75 0.7071291028220443 2202 76-77 1.1508587628947708 2202 78-79 1.1979591068727906 2202 80-81 1.97380633206134 2202 82-83 1.8131217305345153 2202 84-85 1.5924425515338996 2202 86-87 1.6548607910017985 2202 88-89 1.4166432915304092 2202 90-91 1.2160546289358258 2202 92-93 1.410139672923556 2202 94-95 1.230157100974992 2202 96-97 1.1796632991828062 2202 98-99 1.4539187871524888 2202 100-101 1.346097889549256 2305 1 1.359065852444619 2305 2 0.7231808452267714 2305 3 0.11999835446243523 2305 4 -0.24352186502515138 2305 5 0.23091848996138253 2305 6 0.2362153426517466 2305 7 0.5893586243573239 2305 8 -0.06458040291094136 2305 9 0.5000858335319052 2305 10-11 0.2984937822875864 2305 12-13 0.9519543027809121 2305 14-15 0.690194670882633 2305 16-17 0.726135636877359 2305 18-19 0.7409282454169741 2305 20-21 0.8113420232055049 2305 22-23 0.47609467264117455 2305 24-25 0.7046914761418819 2305 26-27 0.9560942356542483 2305 28-29 -2.780414358261453 2305 30-31 -1.3767127505809924 2305 32-33 0.05826940968795924 2305 34-35 -0.8049392912603821 2305 36-37 0.16109297935083333 2305 38-39 -3.032772700826996 2305 40-41 -4.727231188385385 2305 42-43 -4.782720174132912 2305 44-45 -1.9931180131758595 2305 46-47 -0.5521942965169444 2305 48-49 0.3192620426779058 2305 50-51 0.6748912053500469 2305 52-53 0.35247525776773614 2305 54-55 0.8508439748240164 2305 56-57 0.3423282596880597 2305 58-59 -4.450190660649572 2305 60-61 -0.8587578699935037 2305 62-63 0.2862457353843837 2305 64-65 0.5932082217403973 2305 66-67 0.6542801693159674 2305 68-69 0.3036110861734862 2305 70-71 0.6922905552286096 2305 72-73 0.290603251150916 2305 74-75 0.7369211677525556 2305 76-77 0.11820526181105251 2305 78-79 0.5437331553942819 2305 80-81 0.7769352802479013 2305 82-83 0.8741815037363452 2305 84-85 0.9828662491422229 2305 86-87 1.1756576053320238 2305 88-89 0.7598684514423013 2305 90-91 0.7858340376201269 2305 92-93 0.7053127151191312 2305 94-95 0.5484567320917186 2305 96-97 0.8089764001615727 2305 98-99 1.0368968525989537 2305 100-101 1.1348201599122518 1103 1 1.295230672908751 1103 2 0.6851403524576618 1103 3 0.24023244386491527 1103 4 -0.2018662024276523 1103 5 0.1697385762332857 1103 6 0.3299259496922531 1103 7 0.5647009634759925 1103 8 0.24040493204189772 1103 9 0.09026806275507226 1103 10-11 0.5362704438836516 1103 12-13 0.14858939167449137 1103 14-15 0.25011695045159854 1103 16-17 0.44110561851182695 1103 18-19 0.4400707918072797 1103 20-21 0.5794456467099423 1103 22-23 0.20693854093492092 1103 24-25 0.5728223869810805 1103 26-27 0.5758071365033075 1103 28-29 0.33260868880802263 1103 30-31 -0.026112493540644266 1103 32-33 -0.15626815108253567 1103 34-35 0.3424073772588372 1103 36-37 0.8039135065206366 1103 38-39 0.0406519161992378 1103 40-41 -3.256482600168404 1103 42-43 -0.9994957420301809 1103 44-45 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-4.936905662621211 1208 36-37 -2.691628087631514 1208 38-39 -0.5137413233692172 1208 40-41 -0.7015110367540842 1208 42-43 0.031352297088513836 1208 44-45 0.18809092944812988 1208 46-47 -0.017679497263454635 1208 48-49 -1.3538246957289957 1208 50-51 -0.3847520826685624 1208 52-53 -1.6305216921402454 1208 54-55 -0.875634656742335 1208 56-57 -6.4183695418521935 1208 58-59 -1.9521902013568173 1208 60-61 -1.422428055748309 1208 62-63 0.30164837664116106 1208 64-65 -0.011120693326024167 1208 66-67 0.8324380495828727 1208 68-69 0.5277318801677495 1208 70-71 -4.161725183634353 1208 72-73 -0.7141268981839168 1208 74-75 -5.635217551596931 1208 76-77 -1.2124864912964526 1208 78-79 -3.7463740991671095 1208 80-81 0.9686887920585185 1208 82-83 0.6016761587977868 1208 84-85 -2.7965309129460714 1208 86-87 -1.0988264180725018 1208 88-89 -5.049568559144074 1208 90-91 -0.15923479160382925 1208 92-93 -2.0872219986800324 1208 94-95 -4.558912005597133 1208 96-97 -3.238453824536087 1208 98-99 -6.423692190357855 1208 100-101 -3.6034020536847535 1209 1 1.8081570216531517 1209 2 1.3079534586980373 1209 3 0.5849092720251079 1209 4 0.39270778833896003 1209 5 0.33736910427624167 1209 6 1.1170282707666672 1209 7 0.9062752842407491 1209 8 0.032051780231597604 1209 9 0.3606933404816459 1209 10-11 -2.771601750394062 1209 12-13 -0.2061728817306161 1209 14-15 -1.280391968355083 1209 16-17 -0.3458172701725175 1209 18-19 -16.797069613545908 1209 20-21 -14.038554439889971 1209 22-23 -3.279809212551875 1209 24-25 -2.254072592913964 1209 26-27 -1.8224012294416667 1209 28-29 -3.065519473810859 1209 30-31 -0.8857461759197278 1209 32-33 -0.5426044565860195 1209 34-35 -3.0305310364276252 1209 36-37 -4.174868859375792 1209 38-39 -1.4511271608687792 1209 40-41 -10.146013917517056 1209 42-43 -5.555970036618145 1209 44-45 -2.8761108278750136 1209 46-47 -3.0118966109583383 1209 48-49 -2.516043980098978 1209 50-51 -2.190414311767153 1209 52-53 -0.5496802091534931 1209 54-55 -2.836273851283316 1209 56-57 -1.0524889751674351 1209 58-59 -2.0519454231005163 1209 60-61 -2.393413750075691 1209 62-63 -1.0056487864712196 1209 64-65 -0.2089101840256049 1209 66-67 0.08250732644786396 1209 68-69 -1.2850427737365777 1209 70-71 -1.2229676642079639 1209 72-73 -0.6662340694940596 1209 74-75 0.6144158583258275 1209 76-77 -15.769628936077016 1209 78-79 -15.84773192572093 1209 80-81 -21.02263139406532 1209 82-83 -10.085336738245246 1209 84-85 -3.214645658526784 1209 86-87 -2.455374236428561 1209 88-89 -1.7961820750604325 1209 90-91 -5.36417885053535 1209 92-93 -3.0525922257926865 1209 94-95 -0.38023600194855334 1209 96-97 0.19192234884081572 1209 98-99 1.0273648591404623 1209 100-101 0.518480198737052 1212 1 -28.617659002085723 1212 2 -16.263216104256145 1212 3 -5.808061410232202 1212 4 -1.3586251705209094 1212 5 -0.4706936425748012 1212 6 -2.642920028369005 1212 7 -0.2861207479090737 1212 8 -1.189201745161526 1212 9 -0.016375943970160733 1212 10-11 0.8977612231996801 1212 12-13 0.27627955670919135 1212 14-15 -0.20888052681852542 1212 16-17 0.029016937095590833 1212 18-19 -1.091058726367315 1212 20-21 -0.8176163250599444 1212 22-23 -0.5619119790480696 1212 24-25 0.4279156258765653 1212 26-27 -0.2816007777208043 1212 28-29 0.3825083646636358 1212 30-31 -0.5669550585869914 1212 32-33 0.21697623117075437 1212 34-35 -1.3290527975508155 1212 36-37 -1.4772674501008112 1212 38-39 -0.5581822114079955 1212 40-41 -0.22842503643786927 1212 42-43 0.32288878002612265 1212 44-45 1.2196188513031814 1212 46-47 -2.0185724335990827 1212 48-49 -1.6878170138927544 1212 50-51 -0.3407548669485614 1212 52-53 -1.0720780352468395 1212 54-55 -0.5152410397620812 1212 56-57 -1.859968690371545 1212 58-59 -0.7403116993148835 1212 60-61 0.1916765205745108 1212 62-63 -4.813481408599067 1212 64-65 -4.493467124087303 1212 66-67 -2.0047577331198845 1212 68-69 0.24360150490696597 1212 70-71 -0.6867241231398893 1212 72-73 -0.9219813057917108 1212 74-75 -0.16674508781763464 1212 76-77 1.1833947410366754 1212 78-79 -1.1023020703833595 1212 80-81 0.6940563980019157 1212 82-83 0.4970979078038127 1212 84-85 -3.241034273749424 1212 86-87 -1.502366734215542 1212 88-89 -0.5580743031677038 1212 90-91 0.27463855836319695 1212 92-93 -2.1674002003128088 1212 94-95 -1.9093383243702 1212 96-97 0.04336544406980636 1212 98-99 -0.3984805735087207 1212 100-101 0.07730022502861544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 4.0 10 10.0 11 26.0 12 110.0 13 511.0 14 1468.0 15 3381.0 16 6051.0 17 9990.0 18 14793.0 19 20969.0 20 28532.0 21 37171.0 22 46879.0 23 58834.0 24 70494.0 25 84915.0 26 100429.0 27 117658.0 28 136116.0 29 156774.0 30 180169.0 31 203774.0 32 230133.0 33 254871.0 34 275709.0 35 289893.0 36 284747.0 37 245099.0 38 86317.0 39 199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.535960673295165 22.55102706940789 12.898717602993182 24.014294654303757 2 24.38606287625872 24.112364623236346 30.522192369511313 20.97938013099362 3 20.758473887321237 23.436017597973674 30.210239506099924 25.595269008605165 4 25.90441296023424 23.506471597171377 27.00433499081984 23.58478045177454 5 23.710568942318268 26.323680562438646 26.013534155140412 23.952216340102677 6 23.05881003167655 26.20484258805415 26.1126846044912 24.6236627757781 7 25.103054010348853 25.81153335949285 26.372492046918765 22.712920583239534 8 22.763972372292457 25.677624245254965 27.389522434953093 24.16888094749948 9 23.20924987814101 24.986999444676016 26.97163774410834 24.832112933074633 10-11 24.29043444257828 25.15889190462548 25.902486330747703 24.648187322048535 12-13 24.45942584182789 25.1346429803621 25.55488313654166 24.851048041268353 14-15 24.08253147670889 25.687981527667343 25.66515417795659 24.564332817667182 16-17 24.437579811121317 25.567359058905954 25.49159577436391 24.503465355608824 18-19 24.701717624601994 25.547115098536533 25.08591351404071 24.66525376282076 20-21 24.209651810785882 25.690578244771984 25.5597070970994 24.540062847342735 22-23 24.400056618606474 25.325998259351234 25.578507739912858 24.69543738212943 24-25 24.452177643932778 25.186946627798513 25.57535094710573 24.785524781162977 26-27 24.285105233215706 25.544750423281787 25.70099469523032 24.469149648272182 28-29 24.676530569295334 25.451845671527902 25.414473317972536 24.457150441204227 30-31 24.667654211025827 25.213355066550626 25.3009136369376 24.81807708548595 32-33 24.28727764977115 25.441628524915583 25.64186423211185 24.629229593201423 34-35 24.752039016601334 25.340814819139535 25.39940217811915 24.50774398613998 36-37 24.677362197507964 25.2855370010061 25.372077251132712 24.665023550353222 38-39 24.395813617521625 25.429883897912713 25.656137687761284 24.518164796804378 40-41 24.762624712827837 25.315278373695843 25.33703692640246 24.58505998707386 42-43 24.50300879692929 25.156549768026643 25.541848210539754 24.798593224504316 44-45 24.30878117926917 25.350404001591293 25.740149109241322 24.60066570989821 46-47 24.70244127677706 25.318286064470346 25.457996153304236 24.521276505448352 48-49 24.673679051033496 25.20546322401771 25.45303401938747 24.66782370556132 50-51 24.382919032493756 25.447471213354255 25.643803392873966 24.525806361278025 52-53 24.598183076888873 25.35593305486767 25.540181352870622 24.505702515372835 54-55 24.485449264893578 25.1671097581508 25.599123955182137 24.748317021773488 56-57 24.36841379027083 25.440011296767402 25.655814177680703 24.535760735281066 58-59 24.603467442947693 25.292549360166127 25.576383905759048 24.52759929112713 60-61 24.69953409178278 25.165238640697375 25.372556001570285 24.762671265949564 62-63 24.34816627078788 25.42896203382409 25.645172103317147 24.577699592070886 64-65 24.67820602926972 25.338554294368308 25.44021944737267 24.543020228989302 66-67 24.70875815737693 25.22502216233437 25.39441147651641 24.67180820377229 68-69 24.45388216655807 25.402450183078873 25.58468889321511 24.55897875714795 70-71 24.831333664866996 25.319282190023102 25.260030449047065 24.58935369606284 72-73 24.557326648891593 25.256710814588235 25.488158025201123 24.697804511319045 74-75 24.338102294184797 25.351321825819085 25.72783660803563 24.58273927196049 76-77 24.614363135247345 25.450384217488555 25.48992276893609 24.44532987832801 78-79 24.735019754608036 25.31996117475721 25.20377057669131 24.74124849394344 80-81 24.380295817278196 25.404800221301375 25.599203559716965 24.615700401703464 82-83 24.66949899494835 25.42328349008111 25.319409199697812 24.587808315272728 84-85 24.568543168596634 25.18259083714871 25.412455605107166 24.83641038914749 86-87 24.348668550470972 25.516733586528943 25.555636272783193 24.578961590216892 88-89 24.70882332373551 25.371188734085 25.289241721959964 24.630746220219528 90-91 24.724532076301916 25.25448571172478 25.137793377497836 24.883188834475472 92-93 24.345690772522136 25.458345752557456 25.45167501668533 24.744288458235083 94-95 24.720455080151996 25.372633199924906 25.15844867804118 24.74846304188192 96-97 24.693716958451287 25.31575440053257 25.117874287568792 24.872654353447356 98-99 24.44567600208101 25.45810690731265 25.302557217215366 24.793659873390972 100-101 24.80720723779132 25.25227029687845 24.99762361448237 24.94289885084786 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 5.5 3 10.0 4 19.0 5 39.0 6 78.5 7 131.5 8 196.5 9 272.0 10 354.5 11 497.0 12 682.5 13 943.5 14 1258.5 15 1684.5 16 2276.0 17 3006.5 18 3955.5 19 5177.5 20 6740.0 21 8658.5 22 11074.5 23 14052.5 24 17573.0 25 21547.5 26 25904.5 27 30819.5 28 36357.0 29 42306.5 30 48023.5 31 53666.0 32 59440.0 33 64833.5 34 69772.5 35 74211.5 36 77475.0 37 79823.5 38 81199.5 39 81185.0 40 80144.5 41 77887.5 42 74643.5 43 70863.0 44 67006.5 45 63407.0 46 59618.0 47 55975.5 48 52565.0 49 48789.0 50 45319.5 51 42315.5 52 39788.5 53 38038.5 54 37081.0 55 36592.5 56 36705.5 57 37577.5 58 38959.5 59 41234.0 60 44151.0 61 47649.5 62 51887.0 63 56449.0 64 61632.5 65 66702.0 66 71005.5 67 74141.5 68 75357.0 69 74369.0 70 70844.0 71 64583.5 72 56104.5 73 46499.0 74 36398.0 75 26845.0 76 18706.5 77 12312.5 78 7746.5 79 4645.0 80 2736.5 81 1713.0 82 1154.5 83 797.5 84 526.0 85 384.0 86 279.0 87 191.0 88 156.0 89 119.0 90 68.0 91 42.5 92 31.5 93 16.5 94 8.0 95 6.0 96 3.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6849561511295887 2 0.0028173527203407436 3 2.3760806075162898E-4 4 3.394400867880414E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.18894932431056324 14-15 1.697200433940207E-5 16-17 3.394400867880414E-4 18-19 0.11501927340812781 20-21 1.697200433940207E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0020366405207282484 42-43 0.0 44-45 0.0 46-47 0.02182599758047106 48-49 6.788801735760828E-5 50-51 1.697200433940207E-5 52-53 1.1880403037581449E-4 54-55 0.0020366405207282484 56-57 0.0017820604556372172 58-59 0.09382123998821464 60-61 0.0020875565337464545 62-63 0.0017141724382796091 64-65 0.0028003807160013414 66-67 0.005142517314838827 68-69 0.005651677445020889 70-71 0.004175113067492909 72-73 0.03665952937310847 74-75 0.0426506469049174 76-77 0.15597271987910502 78-79 2.206360564122269E-4 80-81 0.1165806978073528 82-83 0.00541406938426926 84-85 0.005617733436342085 86-87 0.007331905874621693 88-89 0.007535569926694519 90-91 0.022946149866871598 92-93 0.01106574682929015 94-95 0.01483353179263741 96-97 0.010352922647035263 98-99 0.01172765499852683 100-101 0.012830835280587963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2946028.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.96390605906834 #Duplication Level Percentage of deduplicated Percentage of total 1 98.8388916347121 96.82643895085371 2 0.8112564794060844 1.589477070766963 3 0.11087315894477785 0.32584703182015107 4 0.09928088460164117 0.38903773010305515 5 0.05900802882563273 0.28903284963025405 6 0.03639485713035011 0.21392294189704988 7 0.009187621958319606 0.06300387341017387 8 0.020930184435907564 0.1640322097502578 9 0.00573225420666932 0.05053986113479673 >10 0.008444895778510311 0.08866748063358937 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.7155206943043313E-4 0.0 0.0 2 3.054960781092373E-4 3.394400867880414E-5 3.394400867880414E-5 3 4.0732810414564966E-4 3.394400867880414E-5 3.394400867880414E-5 4 4.0732810414564966E-4 6.788801735760828E-5 6.788801735760828E-5 5 4.412721128244538E-4 6.788801735760828E-5 6.788801735760828E-5 6 5.091601301820621E-4 6.788801735760828E-5 6.788801735760828E-5 7 5.770481475396704E-4 1.0183202603641242E-4 6.788801735760828E-5 8 7.128241822548869E-4 1.3577603471521656E-4 6.788801735760828E-5 9 7.128241822548869E-4 1.3577603471521656E-4 1.0183202603641242E-4 10-11 7.46768190933691E-4 1.527480390546186E-4 1.0183202603641242E-4 12-13 0.0010183202603641242 1.6972004339402068E-4 1.0183202603641242E-4 14-15 0.0012898723297945572 1.8669204773342276E-4 1.0183202603641242E-4 16-17 0.0013238163384733613 2.0366405207282483E-4 1.0183202603641242E-4 18-19 0.0013917043558309696 2.0366405207282483E-4 2.0366405207282483E-4 20-21 0.0014765643775279799 2.0366405207282483E-4 2.0366405207282483E-4 22-23 0.0015783964035643925 2.206360564122269E-4 2.0366405207282483E-4 24-25 0.0016462844209220008 2.3760806075162898E-4 2.0366405207282483E-4 26-27 0.0017990324599766194 2.7155206943043313E-4 2.0366405207282483E-4 28-29 0.002138472546764661 2.885240737698352E-4 2.0366405207282483E-4 30-31 0.0026136886682679187 3.054960781092373E-4 2.206360564122269E-4 32-33 0.003224680824486393 3.054960781092373E-4 2.3760806075162898E-4 34-35 0.004158141063153507 3.054960781092373E-4 2.3760806075162898E-4 36-37 0.0056177334363420855 3.054960781092373E-4 2.7155206943043313E-4 38-39 0.007569513935373323 3.054960781092373E-4 2.7155206943043313E-4 40-41 0.010217146612320045 3.054960781092373E-4 2.7155206943043313E-4 42-43 0.013967959571327904 3.224680824486393E-4 2.885240737698352E-4 44-45 0.019348084946918358 3.3944008678804137E-4 3.054960781092373E-4 46-47 0.02715520694304331 3.3944008678804137E-4 3.3944008678804137E-4 48-49 0.03598064919953239 3.3944008678804137E-4 3.3944008678804137E-4 50-51 0.04830232434993829 3.3944008678804137E-4 3.3944008678804137E-4 52-53 0.06333952019464852 3.3944008678804137E-4 3.3944008678804137E-4 54-55 0.08080371265989325 3.5641209112744344E-4 3.3944008678804137E-4 56-57 0.1033085904139404 3.733840954668455E-4 3.3944008678804137E-4 58-59 0.13059957339169892 3.733840954668455E-4 3.3944008678804137E-4 60-61 0.16250694154977482 3.733840954668455E-4 3.3944008678804137E-4 62-63 0.19858942277534364 3.733840954668455E-4 3.3944008678804137E-4 64-65 0.2427675500708072 3.733840954668455E-4 3.5641209112744344E-4 66-67 0.2919184746377156 3.733840954668455E-4 3.733840954668455E-4 68-69 0.34796003296642125 3.733840954668455E-4 3.903560998062476E-4 70-71 0.4143205699334833 3.733840954668455E-4 4.0732810414564966E-4 72-73 0.4879960407708277 4.0732810414564966E-4 4.0732810414564966E-4 74-75 0.569224053539206 4.0732810414564966E-4 4.0732810414564966E-4 76-77 0.6610595690197106 4.0732810414564966E-4 4.2430010848505174E-4 78-79 0.7681698884056771 4.0732810414564966E-4 4.7521612150325796E-4 80-81 0.8901816276016385 4.412721128244538E-4 4.7521612150325796E-4 82-83 1.0220031853057745 4.582441171638559E-4 4.7521612150325796E-4 84-85 1.1643813297090184 4.7521612150325796E-4 4.7521612150325796E-4 86-87 1.3170614807462795 4.7521612150325796E-4 4.7521612150325796E-4 88-89 1.4884787245742404 4.7521612150325796E-4 4.7521612150325796E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTACAC 680 0.0054380116 7.0257497 1 GGCTAAG 850 9.084207E-4 6.7447195 1 AATTCGT 960 0.0036997613 5.9537773 6 GTTTCCT 2270 4.9330993E-9 5.6825004 1 GTAATTC 1310 6.123478E-4 5.4704313 1 TCGGAAG 6370 0.0 5.438949 94-95 CGTCTGA 3200 0.0 5.413638 92-93 GTTCAAT 1825 1.4169269E-5 5.235627 1 CTGAACT 3765 0.0 5.231361 94-95 ACGTCTG 3525 0.0 5.1848083 90-91 GTTAATT 1865 2.0639241E-5 5.123335 1 GTTATAA 1500 6.7377574E-4 5.0960107 1 GTTTGGA 1985 1.127352E-5 5.0542927 1 GTCGCGA 1805 6.565833E-5 5.028958 1 GTCTGAA 3735 0.0 5.0194116 92-93 >>END_MODULE