FastQCFastQC Report
Thu 11 Sep 2014
NYCLib26_ATGAGC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib26_ATGAGC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946028
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACAC6800.00543801167.02574971
GGCTAAG8509.084207E-46.74471951
AATTCGT9600.00369976135.95377736
GTTTCCT22704.9330993E-95.68250041
GTAATTC13106.123478E-45.47043131
TCGGAAG63700.05.43894994-95
CGTCTGA32000.05.41363892-93
GTTCAAT18251.4169269E-55.2356271
CTGAACT37650.05.23136194-95
ACGTCTG35250.05.184808390-91
GTTAATT18652.0639241E-55.1233351
GTTATAA15006.7377574E-45.09601071
GTTTGGA19851.127352E-55.05429271
GTCGCGA18056.565833E-55.0289581
GTCTGAA37350.05.019411692-93
Produced by FastQC (version 0.11.2)