##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib25_ACTGAT_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3044427 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.848697636698137 27.0 22.0 33.0 2.0 33.0 2 24.919773408920626 33.0 15.0 33.0 2.0 33.0 3 25.76975700189231 33.0 22.0 33.0 6.0 33.0 4 27.899039129530777 33.0 22.0 37.0 2.0 37.0 5 27.951470999304632 33.0 15.0 37.0 6.0 37.0 6 28.999868612385846 33.0 22.0 37.0 15.0 37.0 7 28.66194492428296 33.0 27.0 37.0 6.0 37.0 8 28.443187174466658 33.0 22.0 37.0 6.0 37.0 9 29.207789183317583 33.0 27.0 37.0 15.0 37.0 10-11 28.829927930608946 33.0 24.5 37.0 10.5 37.0 12-13 29.140240347362575 33.0 27.0 37.0 10.5 37.0 14-15 29.498751653431007 33.0 27.0 37.0 6.0 40.0 16-17 30.3739606829134 33.0 27.0 37.0 15.0 40.0 18-19 30.715885945039904 33.0 27.0 37.0 15.0 40.0 20-21 30.728264793342063 33.0 27.0 37.0 15.0 40.0 22-23 30.75218702895487 33.0 27.0 37.0 15.0 40.0 24-25 30.37500094434848 33.0 27.0 37.0 10.5 40.0 26-27 29.886454166908912 33.0 27.0 37.0 15.0 40.0 28-29 30.086311808428974 33.0 27.0 37.0 15.0 40.0 30-31 30.019355859082843 33.0 27.0 37.0 15.0 40.0 32-33 29.871872933724475 33.0 27.0 37.0 15.0 40.0 34-35 29.92280517811726 33.0 27.0 37.0 15.0 40.0 36-37 30.1668906825488 33.0 27.0 37.0 15.0 40.0 38-39 30.209379137683378 33.0 27.0 37.0 15.0 40.0 40-41 30.297312926209102 33.0 27.0 37.0 15.0 40.0 42-43 30.227469077103834 33.0 27.0 37.0 15.0 40.0 44-45 30.0024671309248 33.0 27.0 37.0 15.0 40.0 46-47 29.643763834705183 33.0 27.0 37.0 15.0 40.0 48-49 29.501728404064213 33.0 27.0 37.0 10.5 40.0 50-51 28.750551581627676 33.0 24.5 37.0 10.5 38.5 52-53 28.47855015081656 33.0 22.0 37.0 10.5 37.0 54-55 29.449630751533867 33.0 27.0 37.0 15.0 40.0 56-57 29.3685419292366 33.0 27.0 37.0 15.0 40.0 58-59 29.564199437201154 33.0 27.0 37.0 15.0 40.0 60-61 29.328362282951765 33.0 24.5 37.0 10.5 40.0 62-63 29.21594950379825 33.0 24.5 37.0 15.0 40.0 64-65 29.183484281278545 33.0 27.0 37.0 15.0 40.0 66-67 29.0684936771353 33.0 27.0 37.0 15.0 40.0 68-69 28.795118917287226 33.0 24.5 37.0 15.0 40.0 70-71 28.711816706395 33.0 24.5 37.0 15.0 38.5 72-73 28.48185553471967 33.0 22.0 37.0 15.0 37.0 74-75 28.44542404859765 33.0 22.0 37.0 15.0 37.0 76-77 28.060136767936953 33.0 22.0 37.0 15.0 37.0 78-79 27.524128185697997 33.0 22.0 37.0 10.5 37.0 80-81 27.400167420667337 30.0 22.0 35.0 10.5 37.0 82-83 26.986187712827405 27.0 22.0 33.0 6.0 37.0 84-85 27.093545846229848 27.0 22.0 33.0 15.0 37.0 86-87 26.79157243711214 27.0 22.0 33.0 6.0 37.0 88-89 26.449866099597724 27.0 22.0 33.0 6.0 37.0 90-91 26.338978237941 27.0 22.0 33.0 6.0 37.0 92-93 26.246730501339 27.0 22.0 33.0 6.0 37.0 94-95 26.044777884311234 27.0 22.0 33.0 6.0 37.0 96-97 25.937542926797065 27.0 22.0 33.0 6.0 37.0 98-99 25.72848453912674 27.0 22.0 33.0 6.0 37.0 100-101 25.26661864449369 27.0 18.5 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.255440296834898 2306 2 8.439112600872857 2306 3 7.418104126122223 2306 4 8.97442362804236 2306 5 8.771528914601983 2306 6 7.8048392743036 2306 7 8.053647543835883 2306 8 8.443071607306795 2306 9 7.3690698936841095 2306 10-11 7.689244975514406 2306 12-13 7.2738634643832185 2306 14-15 7.886808747842029 2306 16-17 6.686596523669273 2306 18-19 6.085875685093118 2306 20-21 5.965409035325624 2306 22-23 5.597729581317655 2306 24-25 5.668145387079605 2306 26-27 6.342012984909292 2306 28-29 5.431747790955107 2306 30-31 5.336022001177412 2306 32-33 5.2627413500732985 2306 34-35 4.721114574624753 2306 36-37 4.7525721398120915 2306 38-39 4.4721819768726405 2306 40-41 3.746948272972041 2306 42-43 3.5222271868262105 2306 44-45 3.1358561698192773 2306 46-47 3.3900084130810058 2306 48-49 3.180451848929877 2306 50-51 3.027175419945422 2306 52-53 3.6928326591093317 2306 54-55 3.1886999726904257 2306 56-57 3.515819721778893 2306 58-59 3.1141811111366238 2306 60-61 3.110667529479308 2306 62-63 2.5868236224417522 2306 64-65 2.262750592428077 2306 66-67 2.0896775374674874 2306 68-69 2.2534220866614127 2306 70-71 2.2977529302098283 2306 72-73 1.4771003148298654 2306 74-75 1.0613669672229733 2306 76-77 1.4859466099139205 2306 78-79 1.790908998907149 2306 80-81 1.4661795743917239 2306 82-83 1.5069547015463698 2306 84-85 1.0917500323717846 2306 86-87 1.0263918811228905 2306 88-89 1.0915052447658162 2306 90-91 1.0025921138296852 2306 92-93 0.9957353155342155 2306 94-95 1.1835479366258994 2306 96-97 0.8721820534906257 2306 98-99 0.6904578006298472 2306 100-101 0.6546796768794252 2307 1 8.274864198920426 2307 2 8.341764969295713 2307 3 7.483118973770946 2307 4 9.025611714261593 2307 5 8.869333931484189 2307 6 7.45414949300638 2307 7 8.065712978646811 2307 8 8.45989859962351 2307 9 7.376227329052828 2307 10-11 7.620561958527048 2307 12-13 7.068249830149732 2307 14-15 8.13444650864744 2307 16-17 6.834974780950795 2307 18-19 6.330193321905231 2307 20-21 6.237070623235365 2307 22-23 5.881219304550168 2307 24-25 6.084179293211378 2307 26-27 6.733386718172827 2307 28-29 6.012218332259913 2307 30-31 5.834871782748948 2307 32-33 5.9171845118109445 2307 34-35 5.692718189267122 2307 36-37 5.473299240257173 2307 38-39 4.963973856043182 2307 40-41 4.513079826111543 2307 42-43 4.221277611681568 2307 44-45 4.355556907972591 2307 46-47 4.660440423651522 2307 48-49 4.3357160204905085 2307 50-51 4.174605187938049 2307 52-53 4.748618532926034 2307 54-55 4.327689178141188 2307 56-57 4.701953435199844 2307 58-59 4.34039369197572 2307 60-61 4.232887087227404 2307 62-63 3.9647266863837984 2307 64-65 3.8768540070443827 2307 66-67 3.5132445964900327 2307 68-69 3.6538949025482843 2307 70-71 3.284785446446225 2307 72-73 2.759055781027744 2307 74-75 2.4002223152340285 2307 76-77 2.65559448403695 2307 78-79 3.0364117844129197 2307 80-81 2.790646384924756 2307 82-83 2.869541187099916 2307 84-85 2.073998748204481 2307 86-87 2.0859985384473916 2307 88-89 2.207281151569674 2307 90-91 2.1623107628879623 2307 92-93 2.033814756741414 2307 94-95 2.2974030788958615 2307 96-97 2.039393530185876 2307 98-99 1.788843830352576 2307 100-101 1.451274581815614 1101 1 7.985121983624417 1101 2 7.846566897505191 1101 3 7.279934071771031 1101 4 8.711979887598517 1101 5 8.436637743059908 1101 6 7.243613128882647 1101 7 7.015245161644547 1101 8 7.941055845232508 1101 9 7.194905918597168 1101 10-11 7.472938483859636 1101 12-13 7.112052441934409 1101 14-15 7.578980422871883 1101 16-17 6.816005010340511 1101 18-19 6.007345803400366 1101 20-21 5.925098699430226 1101 22-23 5.310563671502582 1101 24-25 5.9483957555719655 1101 26-27 6.489543570347937 1101 28-29 5.8372618234649565 1101 30-31 5.631928480912052 1101 32-33 5.799311425513942 1101 34-35 5.411776531313432 1101 36-37 5.310281538602201 1101 38-39 4.887835589473127 1101 40-41 4.584716997312256 1101 42-43 4.336639272458566 1101 44-45 4.309986172547148 1101 46-47 4.551501615170693 1101 48-49 4.235165857599476 1101 50-51 3.742122982852738 1101 52-53 4.717145727464381 1101 54-55 4.3973765521644275 1101 56-57 4.575375674162576 1101 58-59 4.27114851646904 1101 60-61 4.480013343795839 1101 62-63 4.092663223392613 1101 64-65 3.7983187812795514 1101 66-67 3.47571942524867 1101 68-69 3.472136386457162 1101 70-71 3.362348402395817 1101 72-73 3.0259539310980834 1101 74-75 2.4955360083634517 1101 76-77 2.7140524904329943 1101 78-79 3.1879386525128766 1101 80-81 2.794468703924526 1101 82-83 2.868030859689764 1101 84-85 2.402512702663394 1101 86-87 2.3374816901028694 1101 88-89 2.2937089551342638 1101 90-91 2.470176236680338 1101 92-93 2.1257908014283515 1101 94-95 2.4444106442825166 1101 96-97 2.2888509972069997 1101 98-99 2.1213853010481962 1101 100-101 1.8584508723534476 2201 1 7.086074330202397 2201 2 7.811250300222952 2201 3 6.361268115507183 2201 4 8.369964594732707 2201 5 4.144603735814478 2201 6 5.758679567489661 2201 7 6.5534540299296715 2201 8 7.6175792041852155 2201 9 6.418360944244359 2201 10-11 4.175318649155194 2201 12-13 6.36040528249773 2201 14-15 7.042661467656384 2201 16-17 5.937431335812249 2201 18-19 2.0774788844119776 2201 20-21 5.0719417260804995 2201 22-23 5.052949540273463 2201 24-25 5.440267446137902 2201 26-27 6.100920663228283 2201 28-29 4.9368892476770725 2201 30-31 5.193460637325458 2201 32-33 5.309597489466242 2201 34-35 4.881384300248687 2201 36-37 4.997904866197221 2201 38-39 4.3544781035867715 2201 40-41 4.149858882688925 2201 42-43 4.0647673576200525 2201 44-45 3.971960372106558 2201 46-47 4.367674053257119 2201 48-49 4.063749532732224 2201 50-51 3.714000047389508 2201 52-53 4.6546820542048515 2201 54-55 4.151310756162619 2201 56-57 4.398283646150215 2201 58-59 3.9753020064276434 2201 60-61 3.99558577656353 2201 62-63 3.714813613580464 2201 64-65 3.5244560783298375 2201 66-67 3.13383349385051 2201 68-69 3.352748512970418 2201 70-71 3.310448717279744 2201 72-73 2.5779500153275308 2201 74-75 2.264591504897332 2201 76-77 2.750027441995954 2201 78-79 3.052362215694089 2201 80-81 2.5621821957601583 2201 82-83 2.8437912443015136 2201 84-85 2.426368233414319 2201 86-87 2.372997370957858 2201 88-89 2.342738629847844 2201 90-91 2.2267533453407076 2201 92-93 2.168167485023151 2201 94-95 2.2858369835629695 2201 96-97 2.213311185385024 2201 98-99 2.227293047739515 2201 100-101 1.764617358645765 2304 1 1.7010789865034397 2304 2 4.853627041655955 2304 3 5.663068266459497 2304 4 4.369327679215068 2304 5 6.46828409971538 2304 6 6.251966624565281 2304 7 6.981777026293717 2304 8 6.377370890113543 2304 9 5.728366268950634 2304 10-11 6.519393260065669 2304 12-13 6.389644623522578 2304 14-15 6.752822510199071 2304 16-17 5.364904335260668 2304 18-19 5.163123213683928 2304 20-21 4.867905643195368 2304 22-23 4.563853830203104 2304 24-25 4.80151619537579 2304 26-27 5.671359757539651 2304 28-29 5.038565972773284 2304 30-31 4.852010564852542 2304 32-33 5.011246874399717 2304 34-35 4.617044018315394 2304 36-37 4.884831298563785 2304 38-39 4.426125570592355 2304 40-41 3.8119812250945486 2304 42-43 3.356845329877192 2304 44-45 3.211190536944688 2304 46-47 4.28517608123375 2304 48-49 3.901022695993259 2304 50-51 3.660933900271065 2304 52-53 4.194057702722436 2304 54-55 3.9078538784861223 2304 56-57 4.481233561670347 2304 58-59 3.7818356953583674 2304 60-61 3.9216134492311987 2304 62-63 3.6019719069930254 2304 64-65 3.4420721663765192 2304 66-67 3.2445108384948185 2304 68-69 3.476990607692617 2304 70-71 3.4357728953193423 2304 72-73 3.080857825975432 2304 74-75 2.499572045726559 2304 76-77 2.7291284280957377 2304 78-79 3.022673875821283 2304 80-81 2.863069475535358 2304 82-83 2.980488332094925 2304 84-85 2.3205201428583138 2304 86-87 2.384154044332817 2304 88-89 2.765895630499486 2304 90-91 2.6191776924301884 2304 92-93 2.433534307586939 2304 94-95 2.6900908713031626 2304 96-97 2.7262952537232295 2304 98-99 2.4454762150512472 2304 100-101 2.1631590333438595 1102 1 6.912570386003171 1102 2 7.314029827705838 1102 3 6.037486434119867 1102 4 6.149043981861652 1102 5 6.447534009931871 1102 6 5.840454618406998 1102 7 6.7404289505516495 1102 8 6.348365535717928 1102 9 4.820855697845985 1102 10-11 5.9060466312371815 1102 12-13 5.787703187201423 1102 14-15 6.071924586171679 1102 16-17 5.108885504629242 1102 18-19 5.157600184445823 1102 20-21 5.447430667128376 1102 22-23 5.11561749703198 1102 24-25 1.7762702589829118 1102 26-27 5.39945193305056 1102 28-29 4.786007281785324 1102 30-31 4.8175635330452735 1102 32-33 4.974603713901644 1102 34-35 4.827007152410257 1102 36-37 4.702234807699835 1102 38-39 3.9450336623008724 1102 40-41 3.8066405425471643 1102 42-43 3.9806103665299837 1102 44-45 -3.8912397515818853 1102 46-47 1.2348243040762306 1102 48-49 -0.6934988607582575 1102 50-51 2.581504867787043 1102 52-53 -0.14858951906636264 1102 54-55 2.593694991512532 1102 56-57 -0.6655620102700546 1102 58-59 2.828161020385785 1102 60-61 3.7557187292427017 1102 62-63 3.347695562065727 1102 64-65 3.0176978619902286 1102 66-67 3.009398500702762 1102 68-69 3.3703959951478346 1102 70-71 3.197185037014581 1102 72-73 2.6626955396444316 1102 74-75 2.544087987534244 1102 76-77 2.7516137764279556 1102 78-79 -1.4453179793598636 1102 80-81 1.4225524856048253 1102 82-83 2.583062793106645 1102 84-85 2.283943138629134 1102 86-87 2.3011488670951685 1102 88-89 2.227488858766037 1102 90-91 2.093751262463364 1102 92-93 2.0759500380281253 1102 94-95 2.5020597448713033 1102 96-97 2.4311102441036994 1102 98-99 2.196667305858785 1102 100-101 1.7907483470865344 2202 1 -20.585604479284505 2202 2 -7.732590721307176 2202 3 -0.5935045809720307 2202 4 1.5091771902063513 2202 5 1.0564453901587108 2202 6 1.7044293591438802 2202 7 3.8475622273452856 2202 8 2.791554632984454 2202 9 3.0000350928770594 2202 10-11 2.011451700077984 2202 12-13 1.4762507095177426 2202 14-15 2.686917085248197 2202 16-17 1.5940475556629572 2202 18-19 1.5420806176093755 2202 20-21 1.034674985173364 2202 22-23 1.3697323508453323 2202 24-25 2.4005990507511754 2202 26-27 3.4151115976390933 2202 28-29 -1.009674192327477 2202 30-31 0.945520227927787 2202 32-33 1.5206341279972797 2202 34-35 1.4019709977985535 2202 36-37 2.9556778330085756 2202 38-39 2.315400205628105 2202 40-41 1.7187459596018684 2202 42-43 0.6889798216370444 2202 44-45 0.5618317704057674 2202 46-47 -1.243795364003347 2202 48-49 0.8435942602987225 2202 50-51 1.5992455478950909 2202 52-53 1.6058486246218173 2202 54-55 -0.7929858049703888 2202 56-57 1.0231719405339845 2202 58-59 1.8191677021112191 2202 60-61 2.0025476753494544 2202 62-63 -2.16159329799385 2202 64-65 0.008027169241742627 2202 66-67 0.42323995209460463 2202 68-69 1.2354216605802293 2202 70-71 -2.4656938785674463 2202 72-73 -4.511720944114707 2202 74-75 -1.2032579560197547 2202 76-77 -0.322913998880896 2202 78-79 1.3069406166371422 2202 80-81 1.4040054325066933 2202 82-83 -0.8461045613331883 2202 84-85 -0.33628391627289744 2202 86-87 0.43266103911128795 2202 88-89 0.9730413927413011 2202 90-91 1.1252545572799413 2202 92-93 1.07882697311533 2202 94-95 1.5299101432251447 2202 96-97 0.9875289336991564 2202 98-99 0.9763300138709461 2202 100-101 0.7107824752302427 2305 1 5.647582946103476 2305 2 6.968984847281085 2305 3 6.6457272322390715 2305 4 7.570131261779764 2305 5 7.696674218722055 2305 6 6.062786723686212 2305 7 2.9488680984444535 2305 8 6.136587414660795 2305 9 6.22237948975469 2305 10-11 4.561920287771265 2305 12-13 5.157306783006966 2305 14-15 5.6588866888176135 2305 16-17 5.044133807405778 2305 18-19 4.359436036935183 2305 20-21 5.0545581168182885 2305 22-23 5.035916472171781 2305 24-25 5.269718045078903 2305 26-27 6.054548991703815 2305 28-29 5.0817880294759945 2305 30-31 5.288287919892625 2305 32-33 5.378160632491287 2305 34-35 5.2491487966701165 2305 36-37 4.699753810052211 2305 38-39 4.618375531582174 2305 40-41 4.123480425183537 2305 42-43 4.006494697264468 2305 44-45 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1209 42-43 -23.76258443880631 1209 44-45 -26.521606516747887 1209 46-47 -20.07357282602262 1209 48-49 -19.850747921449 1209 50-51 -22.027283907349727 1209 52-53 -24.700301669248876 1209 54-55 -21.808557543149178 1209 56-57 -25.567613114042 1209 58-59 -25.862087545579797 1209 60-61 -25.602616052610244 1209 62-63 -25.757206228304515 1209 64-65 -25.631876273243563 1209 66-67 -13.343978753726116 1209 68-69 -25.350010749159484 1209 70-71 -25.215903786208084 1209 72-73 -15.706300841780248 1209 74-75 -17.056203301093564 1209 76-77 -13.901646407902977 1209 78-79 -14.565534212386805 1209 80-81 -24.314193559936633 1209 82-83 -16.28784352952052 1209 84-85 -13.53885017173588 1209 86-87 -13.906686955463407 1209 88-89 -10.99859595861897 1209 90-91 -13.814144927459594 1209 92-93 -17.62271665538044 1209 94-95 -23.072944600687535 1209 96-97 -18.835229180935535 1209 98-99 -10.32144550336301 1209 100-101 -5.8234665577593425 1212 1 -20.585604479284505 1212 2 -20.42297695136595 1212 3 -21.298164530594196 1212 4 -23.249009207778535 1212 5 -23.448172577935328 1212 6 -24.501280128681476 1212 7 -24.067844993477554 1212 8 -23.696480631499174 1212 9 -24.646900255569623 1212 10-11 -24.36963562150052 1212 12-13 -24.66501713263171 1212 14-15 -25.024459908874388 1212 16-17 -26.081015416629235 1212 18-19 -20.715343659878137 1212 20-21 -26.471307382584822 1212 22-23 -26.699956985846523 1212 24-25 -22.824141939538187 1212 26-27 -25.452016865836978 1212 28-29 -20.367956865972275 1212 30-31 -15.515841560871007 1212 32-33 -25.639945217090883 1212 34-35 -21.79938012502189 1212 36-37 -26.087353233322236 1212 38-39 -26.384012052978107 1212 40-41 -20.942472379947105 1212 42-43 -17.12347029748369 1212 44-45 -14.453585315334461 1212 46-47 -17.511121274171778 1212 48-49 -20.33590013672489 1212 50-51 -12.425741620295073 1212 52-53 -5.267439478436156 1212 54-55 -4.244329139698163 1212 56-57 -4.2730018066215045 1212 58-59 -2.895214754060362 1212 60-61 -14.323464109147347 1212 62-63 -12.149432376714408 1212 64-65 -16.47463245698915 1212 66-67 -12.844555097942038 1212 68-69 -8.205576120184222 1212 70-71 -15.520232408116211 1212 72-73 -12.368496436396338 1212 74-75 -8.640070193264343 1212 76-77 -19.2735834781616 1212 78-79 -10.150057985956515 1212 80-81 -8.118963877957835 1212 82-83 -5.515382464214927 1212 84-85 -4.649810275628845 1212 86-87 -3.6563698403053024 1212 88-89 -3.4563906995409184 1212 90-91 -3.875856543941527 1212 92-93 -3.8057034271481314 1212 94-95 -3.5508597950338263 1212 96-97 -13.405850223288025 1212 98-99 -10.616287096791176 1212 100-101 -6.91245259462524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 532.0 3 1154.0 4 540.0 5 3065.0 6 4079.0 7 3606.0 8 7447.0 9 14198.0 10 18443.0 11 22016.0 12 29231.0 13 37367.0 14 42428.0 15 45783.0 16 48489.0 17 53638.0 18 60851.0 19 67254.0 20 72929.0 21 81523.0 22 90560.0 23 98569.0 24 104310.0 25 107839.0 26 113549.0 27 119356.0 28 122327.0 29 123943.0 30 128419.0 31 136873.0 32 148049.0 33 172060.0 34 198174.0 35 220406.0 36 234179.0 37 223463.0 38 87444.0 39 334.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.562531152562105 33.92421339661873 15.286512323582874 20.226743127236283 2 23.92468913163464 30.03012021522451 25.382663978819924 20.66252667432093 3 20.028783279075242 28.358185613234056 29.783747158133096 21.829283949557613 4 22.914880669724255 26.734763814009543 28.245304181655428 22.105051334610774 5 21.8136250900361 28.939607720744682 27.33470744498316 21.912059744236064 6 21.7688872886656 28.48698078448634 27.376721815402448 22.36741011144561 7 22.621545107921907 29.092423192801203 28.039841376338455 20.24619032293843 8 20.872903631637556 27.7914482911615 29.36346003126218 21.97218804593876 9 21.927750904059494 27.86707210366896 28.327232226063636 21.877944766207907 10-11 22.2933775029529 28.151065850421826 27.654175165320442 21.90138148130483 12-13 21.9498367190968 28.207092732238408 27.843862571241253 21.999207977423538 14-15 22.014916777156717 28.421851193026743 27.67601233519084 21.8872196946257 16-17 22.146019293105816 28.487272492889232 27.652945471185458 21.7137627428195 18-19 22.38254337868298 28.41287644485516 27.32972202819352 21.87485814826834 20-21 22.23627480524781 28.420921678450156 27.446423444363806 21.896380071938225 22-23 22.043090638930163 28.151482152428642 27.80487763506958 22.000549573571618 24-25 22.080420225871062 28.198953960978297 27.79077722356451 21.929848589586125 26-27 21.937385837970748 28.32124414217081 27.78656599929572 21.954804020562722 28-29 22.227451544247586 28.255977449454377 27.709099967732385 21.807471038565645 30-31 22.28554420134687 28.12736631555891 27.529601830768165 22.057487652326053 32-33 22.01308695543921 28.249097610503437 27.74085301150211 21.996962422555242 34-35 22.17388382911791 28.16467017355071 27.771549064064782 21.8898969332666 36-37 22.15240074490545 28.308913882798493 27.628776927239606 21.90990844505645 38-39 22.080235666990095 28.222095013168136 27.832565414183204 21.865103905658568 40-41 22.27757383765347 28.264306401105216 27.59646412196589 21.86165563927543 42-43 22.132441042009336 28.22451623048099 27.6823990382916 21.960643689218077 44-45 21.989085774329663 28.115433896776448 27.88897876787717 22.006501561016716 46-47 22.12980405239386 28.376229125590385 27.627517098055037 21.866449723960717 48-49 22.16252926099737 28.1525507973575 27.77059677385292 21.914323167792208 50-51 22.164267786866944 28.216596303752876 27.632869442005575 21.9862664673746 52-53 22.02740220691718 28.195256724737643 27.86751692511047 21.909824143234705 54-55 22.089061584028567 28.133177943357744 27.737277752117116 22.04048272049657 56-57 21.972010841935706 28.228583029641403 27.872848737337254 21.926557391085638 58-59 22.11396157917106 28.210572828217618 27.7691386192524 21.90632697335893 60-61 22.155634776631203 28.043994340844236 27.637159258341526 22.16321162418303 62-63 21.983865250131593 28.167739920326103 27.800043464500824 22.048351365041484 64-65 22.13512190505243 28.160407258898186 27.722556705256086 21.9819141307933 66-67 22.1923107736552 28.13511194217842 27.693537689994457 21.97903959417192 68-69 22.078238195858937 28.129331615438076 27.869494713928546 21.922935474774444 70-71 22.258636275543118 28.199676371967815 27.620949008009728 21.92073834447934 72-73 22.10855342155108 28.127480517892273 27.74484894699062 22.01911711356603 74-75 22.096912139320203 28.05132043061101 27.766018768453034 22.085748661615757 76-77 22.238910296266667 28.287391076489683 27.61611137292796 21.85758725431569 78-79 22.269246381808905 28.10083287084813 27.651762635865897 21.978158111477068 80-81 22.26006045588501 28.120519075263147 27.57041697297901 22.049003495872835 82-83 22.21391542195345 28.127363111809185 27.727805835467578 21.93091563076979 84-85 22.284189536823952 27.932458676602767 27.59536999800649 22.187981788566795 86-87 22.119786499966626 28.11940311267082 27.75079562728047 22.010014760082083 88-89 22.301900246080116 28.073350974745846 27.74358948228169 21.88115929689235 90-91 22.351424969054573 27.991761037201908 27.456491470898147 22.20032252284537 92-93 22.247325953571167 28.032035088556324 27.620030814359524 22.100608143512986 94-95 22.3485415972307 27.996614274824527 27.564851816774848 22.089992311169922 96-97 22.359770946669244 28.052438328529927 27.496093883310607 22.091696841490222 98-99 22.321352269186423 28.00705492878677 27.615917896721413 22.055674905305395 100-101 22.609968811043256 27.994467441896454 27.277019372184373 22.118544374875917 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 230.0 1 248.0 2 264.0 3 270.0 4 304.5 5 368.5 6 448.5 7 534.5 8 667.0 9 895.5 10 1203.0 11 1594.0 12 2117.5 13 2811.0 14 3568.0 15 4456.5 16 5604.5 17 7165.5 18 9009.0 19 11172.0 20 14098.5 21 17764.0 22 21842.5 23 26584.5 24 31946.0 25 37828.0 26 44145.5 27 50454.0 28 57213.5 29 64056.0 30 70131.5 31 75704.5 32 81411.5 33 86639.5 34 91089.5 35 94934.5 36 97911.0 37 99945.5 38 100280.0 39 98775.0 40 95960.0 41 92276.0 42 87936.0 43 83205.0 44 78363.0 45 73651.5 46 69651.0 47 66293.5 48 62949.5 49 59566.0 50 56578.5 51 54115.0 52 52025.0 53 50434.5 54 49648.5 55 49222.0 56 48903.5 57 49021.5 58 49008.0 59 48200.0 60 46900.5 61 45270.0 62 43091.0 63 40465.5 64 37664.0 65 34927.0 66 31684.5 67 28450.5 68 25379.0 69 22014.0 70 18774.5 71 15660.0 72 12813.5 73 10426.5 74 8367.0 75 6505.0 76 5026.5 77 3916.0 78 3077.5 79 2391.5 80 1879.0 81 1502.5 82 1200.0 83 953.5 84 769.5 85 637.0 86 514.5 87 398.0 88 317.5 89 238.5 90 171.5 91 139.5 92 99.5 93 71.0 94 53.5 95 44.0 96 26.0 97 13.0 98 9.5 99 4.5 100 1.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.606041465274089 2 10.969387671308919 3 9.68701171024958 4 11.529920080199 5 9.06880013874532 6 5.30464353390638 7 9.67413572406236 8 9.976754246365573 9 5.362454084134716 10-11 6.339288148475887 12-13 5.360483269922386 14-15 7.321771880225738 16-17 2.6652141765921797 18-19 3.0350867338911396 20-21 3.100616306451099 22-23 3.175868562458551 24-25 3.469503456643894 26-27 4.296539217396245 28-29 1.7675575732313504 30-31 2.2254762554661354 32-33 3.0351688511499866 34-35 1.9867942309012498 36-37 3.0342162909473607 38-39 1.4203986497294894 40-41 1.1753443258780716 42-43 0.568711287871248 44-45 1.2657061575133843 46-47 1.3424201007283145 48-49 1.2367351885921392 50-51 1.5707389272266998 52-53 2.488514259005061 54-55 1.7206850418814443 56-57 1.7919628225606985 58-59 1.578540066817171 60-61 1.3749385352317531 62-63 0.6230893366797758 64-65 1.2606148874648662 66-67 0.5757897955838652 68-69 0.8159335073562283 70-71 1.0212430779256656 72-73 0.08531983194210274 74-75 0.10708090553657552 76-77 0.4703348117724616 78-79 0.8346890892768984 80-81 0.2953100862658228 82-83 0.5074682362231053 84-85 0.0674182695134421 86-87 0.10235095142698446 88-89 0.08315193630854016 90-91 0.09138008564501629 92-93 0.1727582891624598 94-95 0.46172892304528895 96-97 0.38376679749588344 98-99 0.0661865106307361 100-101 0.2343298098459907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3044427.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.94108659274586 #Duplication Level Percentage of deduplicated Percentage of total 1 98.48253527203016 96.45486514951057 2 1.175626946319444 2.3028436110047608 3 0.19619548805441256 0.5764679785392978 4 0.08007610486622037 0.3137096288284915 5 0.04875023933126187 0.23873257058801023 6 0.006422929315844465 0.03774412057813252 7 0.007350126641466331 0.050391557290165166 8 0.0016099499153544648 0.012614419525577243 9 0.0014329435258177796 0.012630964135017035 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.569380707765369E-5 3.2846903538826844E-5 0.0 2 6.569380707765369E-5 3.2846903538826844E-5 0.0 3 6.569380707765369E-5 3.2846903538826844E-5 0.0 4 6.569380707765369E-5 6.569380707765369E-5 0.0 5 6.569380707765369E-5 6.569380707765369E-5 0.0 6 9.854071061648054E-5 6.569380707765369E-5 0.0 7 9.854071061648054E-5 6.569380707765369E-5 0.0 8 9.854071061648054E-5 6.569380707765369E-5 0.0 9 9.854071061648054E-5 6.569380707765369E-5 0.0 10-11 1.3138761415530737E-4 6.569380707765369E-5 0.0 12-13 1.3138761415530737E-4 9.854071061648054E-5 0.0 14-15 1.4781106592472079E-4 9.854071061648054E-5 0.0 16-17 1.8065796946354766E-4 9.854071061648054E-5 0.0 18-19 2.1350487300237449E-4 9.854071061648054E-5 0.0 20-21 2.2992832477178793E-4 9.854071061648054E-5 0.0 22-23 2.2992832477178793E-4 1.3138761415530737E-4 1.6423451769413422E-5 24-25 2.2992832477178793E-4 1.3138761415530737E-4 3.2846903538826844E-5 26-27 2.4635177654120136E-4 1.4781106592472079E-4 9.854071061648052E-5 28-29 2.956221318494416E-4 1.6423451769413422E-4 1.3138761415530737E-4 30-31 3.2846903538826845E-4 1.6423451769413422E-4 1.3138761415530737E-4 32-33 3.7773939069650877E-4 1.6423451769413422E-4 1.3138761415530737E-4 34-35 4.762801013129893E-4 2.1350487300237449E-4 1.3138761415530737E-4 36-37 6.076677154682967E-4 2.2992832477178793E-4 1.4781106592472079E-4 38-39 9.032898473177384E-4 2.2992832477178793E-4 1.6423451769413422E-4 40-41 0.0011332181720895263 2.2992832477178793E-4 1.6423451769413422E-4 42-43 0.0014124168521695544 2.2992832477178793E-4 1.8065796946354766E-4 44-45 0.0019051204052519572 2.2992832477178793E-4 1.9708142123296107E-4 46-47 0.00249636466895084 2.2992832477178793E-4 1.9708142123296107E-4 48-49 0.0030547620291108968 2.4635177654120136E-4 1.9708142123296107E-4 50-51 0.0038595111658121543 2.6277522831061475E-4 1.9708142123296107E-4 52-53 0.005041999693209921 2.6277522831061475E-4 2.2992832477178793E-4 54-55 0.006684344870151264 2.6277522831061475E-4 2.2992832477178793E-4 56-57 0.00854019492009498 2.6277522831061475E-4 2.2992832477178793E-4 58-59 0.01156211004566705 2.6277522831061475E-4 2.2992832477178793E-4 60-61 0.015257386693785069 2.6277522831061475E-4 2.2992832477178793E-4 62-63 0.01900193369721133 2.6277522831061475E-4 2.2992832477178793E-4 64-65 0.02279575105594583 3.2846903538826845E-4 2.4635177654120136E-4 66-67 0.027755633490308684 3.2846903538826845E-4 2.6277522831061475E-4 68-69 0.03440713145692112 3.2846903538826845E-4 2.956221318494416E-4 70-71 0.0419619192708513 3.2846903538826845E-4 2.956221318494416E-4 72-73 0.04958240089185913 3.2846903538826845E-4 3.2846903538826845E-4 74-75 0.058024055101337624 3.613159389270953E-4 3.448924871576819E-4 76-77 0.06797666687360215 3.613159389270953E-4 3.7773939069650877E-4 78-79 0.08083622960905287 3.613159389270953E-4 3.9416284246592215E-4 80-81 0.09658631985592034 3.9416284246592215E-4 4.27009746004749E-4 82-83 0.11412656634565388 3.9416284246592215E-4 4.27009746004749E-4 84-85 0.13296426552517107 4.27009746004749E-4 4.5985664954357585E-4 86-87 0.15571074622580866 4.27009746004749E-4 4.927035530824027E-4 88-89 0.18029665352462054 4.27009746004749E-4 4.927035530824027E-4 >>END_MODULE >>Kmer Content pass >>END_MODULE