##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib25_ACTGAT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3044427 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23634431044003 33.0 33.0 33.0 27.0 33.0 2 31.681121275037963 33.0 33.0 33.0 27.0 33.0 3 31.834408576720676 33.0 33.0 33.0 27.0 33.0 4 35.66074666924187 37.0 37.0 37.0 33.0 37.0 5 35.16836304499993 37.0 37.0 37.0 33.0 37.0 6 34.984760350634126 37.0 37.0 37.0 33.0 37.0 7 34.79068606342015 37.0 33.0 37.0 27.0 37.0 8 34.842250774940574 37.0 33.0 37.0 27.0 37.0 9 34.74628854625189 37.0 33.0 37.0 27.0 37.0 10-11 34.60845521998064 37.0 33.0 37.0 27.0 37.0 12-13 34.61941705286414 37.0 33.0 37.0 27.0 37.0 14-15 35.58409546361269 37.0 33.0 40.0 27.0 40.0 16-17 35.49656881245633 37.0 33.0 40.0 27.0 40.0 18-19 35.71833550287131 37.0 33.0 40.0 27.0 40.0 20-21 35.40557976919795 37.0 33.0 40.0 27.0 40.0 22-23 35.45264839656198 37.0 33.0 40.0 27.0 40.0 24-25 35.3075897369193 37.0 33.0 40.0 27.0 40.0 26-27 34.95163851851268 37.0 33.0 40.0 27.0 40.0 28-29 34.61490076786207 37.0 33.0 40.0 27.0 40.0 30-31 34.52579418064549 37.0 33.0 40.0 27.0 40.0 32-33 34.54313603183785 37.0 33.0 40.0 27.0 40.0 34-35 34.42244336947478 37.0 33.0 40.0 27.0 40.0 36-37 34.14307881253188 37.0 33.0 40.0 22.0 40.0 38-39 34.07394199302529 37.0 33.0 38.5 24.5 40.0 40-41 33.80857169510058 37.0 33.0 37.0 22.0 40.0 42-43 33.456640280749056 37.0 33.0 37.0 22.0 40.0 44-45 33.35567842487273 37.0 33.0 37.0 22.0 40.0 46-47 33.23895432539522 37.0 33.0 37.0 22.0 40.0 48-49 33.25725399229478 37.0 33.0 37.0 22.0 40.0 50-51 33.53859790364493 37.0 33.0 37.0 22.0 40.0 52-53 33.39767269834356 37.0 33.0 37.0 22.0 40.0 54-55 33.04429979763023 37.0 33.0 37.0 22.0 40.0 56-57 33.10184855803736 37.0 33.0 37.0 22.0 40.0 58-59 32.813525172388765 37.0 33.0 37.0 22.0 40.0 60-61 32.73201755207137 37.0 33.0 37.0 22.0 40.0 62-63 32.716326914719914 37.0 33.0 37.0 22.0 40.0 64-65 32.39283385674874 37.0 33.0 37.0 22.0 40.0 66-67 32.052862492679246 33.0 27.0 37.0 22.0 40.0 68-69 31.886498674463205 33.0 27.0 37.0 22.0 40.0 70-71 31.72851147358764 33.0 27.0 37.0 22.0 40.0 72-73 31.5172122701579 33.0 27.0 37.0 22.0 38.5 74-75 31.176402653110095 33.0 27.0 37.0 15.0 37.0 76-77 30.129210521388753 33.0 27.0 37.0 15.0 37.0 78-79 31.040189828824932 33.0 27.0 37.0 22.0 37.0 80-81 30.74083054052536 33.0 27.0 37.0 15.0 37.0 82-83 30.712565944264718 33.0 27.0 37.0 15.0 37.0 84-85 30.593571138345574 33.0 27.0 37.0 15.0 37.0 86-87 30.393828296753377 33.0 27.0 37.0 15.0 37.0 88-89 30.259427307667423 33.0 27.0 37.0 15.0 37.0 90-91 30.172796555805082 33.0 27.0 37.0 15.0 37.0 92-93 29.962967908246775 33.0 27.0 37.0 15.0 37.0 94-95 29.87198017886453 33.0 27.0 37.0 15.0 37.0 96-97 29.745223321170126 33.0 27.0 33.0 15.0 37.0 98-99 29.492932003296517 33.0 27.0 33.0 15.0 37.0 100-101 28.71448469613494 33.0 27.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1336156693601396 2306 2 0.5998785445797665 2306 3 0.44412010068714736 2306 4 0.30878204426503686 2306 5 0.6233032117277446 2306 6 0.6936598848331883 2306 7 0.9035536713675114 2306 8 0.7835950750346612 2306 9 0.9501745552543923 2306 10-11 1.1491667672061112 2306 12-13 1.3264478046459658 2306 14-15 1.449139595971701 2306 16-17 1.388234651670004 2306 18-19 1.288079744309563 2306 20-21 1.4903665034449531 2306 22-23 1.1270233064922266 2306 24-25 1.179820668957177 2306 26-27 1.2565249905759828 2306 28-29 1.5421827098008762 2306 30-31 1.5431415824742203 2306 32-33 1.142312609988366 2306 34-35 1.0497271251636562 2306 36-37 1.2189291319353899 2306 38-39 1.0320851678693117 2306 40-41 1.2231978057235082 2306 42-43 1.2044438571403617 2306 44-45 1.0346045980562693 2306 46-47 0.9807034752929695 2306 48-49 1.0287952144312271 2306 50-51 1.0004951365577455 2306 52-53 0.9754384030956231 2306 54-55 1.4509522339335135 2306 56-57 1.2076543959514368 2306 58-59 1.0006617878233754 2306 60-61 0.9440089893239332 2306 62-63 0.6078274367814132 2306 64-65 0.6449003441051602 2306 66-67 0.8832144066817271 2306 68-69 0.8331364336160938 2306 70-71 0.6875234524651646 2306 72-73 0.470258186855002 2306 74-75 0.6942232578985212 2306 76-77 0.7870145463457092 2306 78-79 0.6835481428269183 2306 80-81 1.2440836127981143 2306 82-83 0.9539844316960782 2306 84-85 0.862369655331257 2306 86-87 0.6786567401735653 2306 88-89 0.4837331882634075 2306 90-91 0.17002334402513597 2306 92-93 0.12321664122952214 2306 94-95 -0.047525733053571884 2306 96-97 0.04952338005621115 2306 98-99 0.1727814371781662 2306 100-101 0.21058254923872965 2307 1 0.5900496504731088 2307 2 0.4608678163248854 2307 3 0.28930310738745035 2307 4 0.3067997745750404 2307 5 0.46967793940655156 2307 6 0.5751817059618816 2307 7 0.7821625644118626 2307 8 0.691841132235254 2307 9 0.9874835532266957 2307 10-11 1.1383112157399822 2307 12-13 1.3571594059288756 2307 14-15 1.3231813454110508 2307 16-17 1.3285176942606896 2307 18-19 1.1765686902979553 2307 20-21 1.400840679509038 2307 22-23 1.0809167737553835 2307 24-25 1.2017610131848784 2307 26-27 1.3078632677915323 2307 28-29 1.586587763716473 2307 30-31 1.5926951128770455 2307 32-33 1.2777964215935356 2307 34-35 1.2632984818889952 2307 36-37 1.3643270009148907 2307 38-39 1.0420150901677587 2307 40-41 1.192160504863736 2307 42-43 1.1238367837616607 2307 44-45 0.9969514409592293 2307 46-47 0.9482801881447003 2307 48-49 1.0100213741823154 2307 50-51 0.9797210471335163 2307 52-53 1.1986995022559768 2307 54-55 1.5215186963068916 2307 56-57 1.2003053699069 2307 58-59 1.205536057156749 2307 60-61 0.9580803769104236 2307 62-63 0.8647717472401482 2307 64-65 0.817230110497583 2307 66-67 1.0819700388440872 2307 68-69 0.8734841589197408 2307 70-71 0.8533964919240162 2307 72-73 0.7565744612038898 2307 74-75 0.8765753471992994 2307 76-77 1.0071453976794835 2307 78-79 1.001053703551399 2307 80-81 1.598422006600277 2307 82-83 1.3425061476682139 2307 84-85 1.0860238228456573 2307 86-87 1.0195961882845204 2307 88-89 0.9176066414904547 2307 90-91 0.6862902633723387 2307 92-93 0.6952727491110053 2307 94-95 0.5861820643051701 2307 96-97 0.6420641453673461 2307 98-99 0.7916833741162392 2307 100-101 0.7160270071452466 1101 1 0.9749385053414983 1101 2 0.5371375803791132 1101 3 0.32733956625288485 1101 4 0.3267776063104364 1101 5 0.5236952004861521 1101 6 0.5672388109673747 1101 7 0.7315790108045661 1101 8 0.6825168079459019 1101 9 0.8923161892744318 1101 10-11 0.9962603997957373 1101 12-13 1.2126390836984058 1101 14-15 1.2514861530037678 1101 16-17 1.2798574736681374 1101 18-19 0.9518861843868578 1101 20-21 1.2566179791336296 1101 22-23 1.002035467313057 1101 24-25 1.1306990905491148 1101 26-27 1.0136561601008083 1101 28-29 1.3064143545261047 1101 30-31 1.3223713474872767 1101 32-33 0.9826843689175746 1101 34-35 1.138565084804867 1101 36-37 1.2042685689845172 1101 38-39 1.0247250058472872 1101 40-41 1.1420356163631382 1101 42-43 1.171835435571353 1101 44-45 0.9341787057087672 1101 46-47 0.907450725067747 1101 48-49 0.9408221287003045 1101 50-51 1.01271482447968 1101 52-53 1.1166375563082127 1101 54-55 1.5228936212986355 1101 56-57 1.1974844195395278 1101 58-59 1.0719145357562638 1101 60-61 0.8401144778542502 1101 62-63 0.7513042853751841 1101 64-65 0.7006316323727759 1101 66-67 0.7639359869236664 1101 68-69 0.6748944000426071 1101 70-71 0.8416770432884988 1101 72-73 0.7864359755340935 1101 74-75 0.9363245812617365 1101 76-77 0.8993864909537983 1101 78-79 0.7639414038295378 1101 80-81 1.4553292201170684 1101 82-83 1.105900007403779 1101 84-85 1.0234977806392926 1101 86-87 0.8435749497706446 1101 88-89 0.8648732451590853 1101 90-91 0.6918959728943044 1101 92-93 0.7943322504052688 1101 94-95 0.8050964883536018 1101 96-97 0.6876553816975175 1101 98-99 0.834136113649901 1101 100-101 0.853284245793585 2201 1 1.4341641207167477 2201 2 0.7856599229151868 2201 3 0.290311735907423 2201 4 0.27605434035704945 2201 5 0.39569370076450383 2201 6 0.4821051565369814 2201 7 0.6312017971931994 2201 8 0.6544185734162582 2201 9 0.5972468360762946 2201 10-11 0.7997276775821476 2201 12-13 1.072519627364649 2201 14-15 1.0934755545295474 2201 16-17 1.0350878874288938 2201 18-19 0.9109095497082436 2201 20-21 1.1937181647632187 2201 22-23 0.9560394719831677 2201 24-25 0.9503325877387141 2201 26-27 1.0156826127889076 2201 28-29 1.2972673547824414 2201 30-31 1.3817193223595154 2201 32-33 1.0064860575852919 2201 34-35 0.8791301912161273 2201 36-37 0.9878052288959438 2201 38-39 0.6208408739151139 2201 40-41 1.1033773220312924 2201 42-43 1.012419943226746 2201 44-45 0.9356031936691238 2201 46-47 0.8571094010200966 2201 48-49 0.9787480968953588 2201 50-51 0.8801340216548112 2201 52-53 1.0345663295769967 2201 54-55 1.4163348889424512 2201 56-57 1.0302659082242016 2201 58-59 1.0427898970833027 2201 60-61 0.8395722596256618 2201 62-63 0.7920839792126486 2201 64-65 0.7683361414014271 2201 66-67 0.7708112388285393 2201 68-69 0.7770529616564126 2201 70-71 0.95999653890091 2201 72-73 0.876585217027344 2201 74-75 0.8656134507877411 2201 76-77 0.9991343842974807 2201 78-79 0.8785974236279976 2201 80-81 1.4380177940080152 2201 82-83 1.1853959564625747 2201 84-85 1.130518640678897 2201 86-87 1.1037451217041436 2201 88-89 1.0078245474649172 2201 90-91 0.7823468990912268 2201 92-93 0.9548032285224792 2201 94-95 0.9576528230883667 2201 96-97 0.7792769297777618 2201 98-99 0.8887136656652466 2201 100-101 0.9855906123181555 2304 1 -1.8338101883642253 2304 2 -0.2877954232434554 2304 3 -0.15792099254797165 2304 4 -0.009630438774308914 2304 5 0.3606747953679914 2304 6 0.48046934403070907 2304 7 0.4825476624510614 2304 8 0.6611760015708086 2304 9 0.8358655806469102 2304 10-11 0.8289680852910095 2304 12-13 1.1503429393619982 2304 14-15 1.2367505670901267 2304 16-17 1.2834769466884168 2304 18-19 0.9907372360715314 2304 20-21 1.1252211264339067 2304 22-23 1.0025340643910425 2304 24-25 1.161299637749579 2304 26-27 1.0406711429310889 2304 28-29 1.5465130063699704 2304 30-31 1.5750246993573427 2304 32-33 1.0705516097945278 2304 34-35 1.0091834151423313 2304 36-37 1.110601936742519 2304 38-39 1.0127161040972652 2304 40-41 1.02070701293421 2304 42-43 1.0814549057932012 2304 44-45 1.107597426123725 2304 46-47 0.9692826573049871 2304 48-49 0.9454835261195313 2304 50-51 0.9366765670675292 2304 52-53 1.1552339061000794 2304 54-55 1.55716758574588 2304 56-57 1.229918400603502 2304 58-59 0.9005639006359374 2304 60-61 0.5348386074669875 2304 62-63 0.4815180745019063 2304 64-65 0.5185871051053539 2304 66-67 0.6988530963502733 2304 68-69 0.7063269744185767 2304 70-71 0.6282096319573114 2304 72-73 0.4715471963529687 2304 74-75 0.5876880766618484 2304 76-77 0.6667586828062646 2304 78-79 0.5502093174731684 2304 80-81 1.290710866141783 2304 82-83 1.026840605373149 2304 84-85 0.6773241038677966 2304 86-87 0.5654516616699823 2304 88-89 0.5440675553888177 2304 90-91 0.5020741290609934 2304 92-93 0.41314104518377803 2304 94-95 0.2956648077489099 2304 96-97 0.236774785367313 2304 98-99 0.6651559283586295 2304 100-101 0.6870935009735639 1102 1 1.4049172096337408 1102 2 0.14504929261259036 1102 3 0.29332591413685094 1102 4 0.1851310224749767 1102 5 0.28948360819837404 1102 6 0.16461559645411938 1102 7 -4.469737174939798 1102 8 -1.0100099227471588 1102 9 0.1904642937684926 1102 10-11 0.7111537772281409 1102 12-13 0.9512153496776321 1102 14-15 0.8379123971837856 1102 16-17 0.5805454641986998 1102 18-19 0.8542590770911644 1102 20-21 1.079217317167057 1102 22-23 0.7625259773894371 1102 24-25 1.1273368253557834 1102 26-27 1.041702587728409 1102 28-29 0.9286968451903235 1102 30-31 1.2076635710019517 1102 32-33 1.0067178521592268 1102 34-35 1.205522064079375 1102 36-37 1.2998679801936603 1102 38-39 0.9057984932984837 1102 40-41 1.360152048934708 1102 42-43 1.2759691612646193 1102 44-45 1.1754428109549266 1102 46-47 1.019128904206113 1102 48-49 0.869209054322674 1102 50-51 0.7737922352810571 1102 52-53 0.9348088159366839 1102 54-55 1.3264886445278492 1102 56-57 1.1568309190373824 1102 58-59 0.9852109393367954 1102 60-61 0.9313991115444011 1102 62-63 0.5757492478546808 1102 64-65 0.46602196527101114 1102 66-67 0.5112678298146243 1102 68-69 0.6847526182932633 1102 70-71 0.5910702067867248 1102 72-73 0.5338923452824034 1102 74-75 0.7023585721148535 1102 76-77 0.7527297734433169 1102 78-79 0.6907380191017225 1102 80-81 1.1584539218646341 1102 82-83 1.000751686159031 1102 84-85 0.7411691655154335 1102 86-87 0.6595266172785692 1102 88-89 0.6817936032059002 1102 90-91 0.175803456153524 1102 92-93 0.39938554466990794 1102 94-95 0.35597375196527636 1102 96-97 0.20581216903327615 1102 98-99 0.10468909859297781 1102 100-101 0.34165363220387945 2202 1 1.5710180779719956 2202 2 0.8821249949475742 2202 3 0.3926214227919935 2202 4 -0.28696428705067945 2202 5 0.11508297788437005 2202 6 0.4727925589938664 2202 7 0.6407209834218506 2202 8 -3.771895482254461 2202 9 -0.5078315101365405 2202 10-11 0.4428825083993857 2202 12-13 0.08085962463919572 2202 14-15 0.13308015764694403 2202 16-17 0.23877496020290323 2202 18-19 0.857914199238941 2202 20-21 1.132788461663928 2202 22-23 -2.7281112223747783 2202 24-25 -2.833171987408349 2202 26-27 -1.477437063135305 2202 28-29 0.5459356077794908 2202 30-31 1.20110967651285 2202 32-33 1.0158185688590038 2202 34-35 -2.097813759832018 2202 36-37 -0.2340484862708081 2202 38-39 0.29293425994043787 2202 40-41 1.2069478213882547 2202 42-43 1.2464394835423604 2202 44-45 1.2653424077483635 2202 46-47 0.6084371620230513 2202 48-49 0.8704112526281449 2202 50-51 -3.8993058584671303 2202 52-53 -0.3617936357028242 2202 54-55 1.3597501210721248 2202 56-57 1.2703839471877032 2202 58-59 1.401850241023375 2202 60-61 1.3025261641550614 2202 62-63 1.1008545555954043 2202 64-65 1.1675741288339054 2202 66-67 1.1442742209604653 2202 68-69 1.116923042135216 2202 70-71 1.224053647835916 2202 72-73 1.0679664340322184 2202 74-75 0.6655029425984473 2202 76-77 1.1517986234413407 2202 78-79 1.1802111126127492 2202 80-81 1.6655675031699317 2202 82-83 1.6398268819135033 2202 84-85 1.344490537068669 2202 86-87 1.3153197726183947 2202 88-89 1.3601711495902755 2202 90-91 1.0811246385600164 2202 92-93 1.1406077712218767 2202 94-95 1.074368385772953 2202 96-97 1.1822569414682924 2202 98-99 1.2213033740930896 2202 100-101 1.1506468373698624 2305 1 1.3039267680647875 2305 2 0.7574193918753238 2305 3 0.1445009857564301 2305 4 -0.1336463430463155 2305 5 0.3226392018923221 2305 6 0.404707808763078 2305 7 0.5684369945051131 2305 8 0.0853267271600231 2305 9 0.42739640462274764 2305 10-11 0.3462690294721753 2305 12-13 0.8441974468052464 2305 14-15 0.7257544227530275 2305 16-17 0.7802130505629208 2305 18-19 0.7594773721670904 2305 20-21 0.9479279896866402 2305 22-23 0.5655901618295402 2305 24-25 0.8176327030960735 2305 26-27 0.9230369789825374 2305 28-29 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-0.0062600939496633146 1208 44-45 0.1100312548597131 1208 46-47 -0.17715105671173603 1208 48-49 -1.565143582836086 1208 50-51 -0.08285785069259788 1208 52-53 -1.524680281905031 1208 54-55 -0.903927993210651 1208 56-57 -6.297110844899702 1208 58-59 -2.0372018821648794 1208 60-61 -1.4502760722486698 1208 62-63 0.34226914950061627 1208 64-65 -0.006367551112631986 1208 66-67 0.7186701092186034 1208 68-69 0.4912800087433773 1208 70-71 -4.196878335968762 1208 72-73 -0.6374024893557824 1208 74-75 -5.657272434194944 1208 76-77 -1.3018023023553909 1208 78-79 -3.936001376341089 1208 80-81 0.8424099475964262 1208 82-83 0.33507250445183345 1208 84-85 -2.641871892033958 1208 86-87 -0.8700046540216348 1208 88-89 -4.898688268592256 1208 90-91 -0.22176443718543126 1208 92-93 -2.2409573503287206 1208 94-95 -4.828325959327447 1208 96-97 -3.516310025800802 1208 98-99 -6.5086781381231695 1208 100-101 -3.9568163890146124 1209 1 1.771756163470787 1209 2 1.1743935762589786 1209 3 0.49505814550579075 1209 4 0.28550228919423404 1209 5 0.21248550538765443 1209 6 0.9497278356772583 1209 7 0.8870502908342814 1209 8 0.3823202525658047 1209 9 0.2934003068383504 1209 10-11 -2.827042570719648 1209 12-13 -0.29682791079392956 1209 14-15 -0.9667210580232819 1209 16-17 -0.3534749626515534 1209 18-19 -16.962456888764386 1209 20-21 -14.214079429284563 1209 22-23 -3.8460757282486 1209 24-25 -2.208234780292301 1209 26-27 -1.6775831014425506 1209 28-29 -3.379005366909592 1209 30-31 -0.9535393292066843 1209 32-33 -0.7050975613770305 1209 34-35 -3.248201819854838 1209 36-37 -4.128582455426944 1209 38-39 -1.7500734833191913 1209 40-41 -10.835385346889495 1209 42-43 -5.698456172625342 1209 44-45 -3.2055871781056524 1209 46-47 -2.7222543849783243 1209 48-49 -2.964771779658161 1209 50-51 -2.835203294364568 1209 52-53 -1.3833405653102133 1209 54-55 -2.8290985803710385 1209 56-57 -0.8718867870225822 1209 58-59 -2.1557469803018137 1209 60-61 -2.174451273464687 1209 62-63 -1.7845457400693725 1209 64-65 -0.38400215196151777 1209 66-67 0.0015836714689037024 1209 68-69 -1.4695373768189093 1209 70-71 -1.3732304702290605 1209 72-73 -0.2757680920070982 1209 74-75 0.2062573183654166 1209 76-77 -16.727738610944762 1209 78-79 -16.760072854749303 1209 80-81 -22.231480872467863 1209 82-83 -9.915099847761336 1209 84-85 -3.4963474429380774 1209 86-87 -2.5339692325270313 1209 88-89 -2.0488625935114797 1209 90-91 -6.249539100164174 1209 92-93 -3.6823342368586722 1209 94-95 -0.97681050577118 1209 96-97 0.36936179481999787 1209 98-99 1.2048148598086037 1209 100-101 0.7728740830228027 1212 1 -28.63280224108762 1212 2 -16.286218707674863 1212 3 -5.459219385626863 1212 4 -1.3036070702331415 1212 5 -0.40446958506566943 1212 6 -2.689898471793576 1212 7 -0.3743130023311281 1212 8 -1.3588810601902779 1212 9 -0.11761617308272321 1212 10-11 0.7970627115128437 1212 12-13 0.4562023007168463 1212 14-15 -0.10806255581354662 1212 16-17 -0.09547032169638214 1212 18-19 -0.9378490041741685 1212 20-21 -0.7238898648900545 1212 22-23 -0.5330465737883472 1212 24-25 0.18176149486298243 1212 26-27 -0.6965357657132358 1212 28-29 -0.08009538734589938 1212 30-31 -0.9287842125787762 1212 32-33 -0.054102927166852055 1212 34-35 -1.1224241326813882 1212 36-37 -1.3882746020448806 1212 38-39 -0.5296507637798626 1212 40-41 -0.2734321188585227 1212 42-43 0.10411950715882057 1212 44-45 0.7522239219315949 1212 46-47 -2.5113488953625875 1212 48-49 -1.3271047908427605 1212 50-51 -0.1783105698923393 1212 52-53 -0.8338076809716739 1212 54-55 -0.12285795540249467 1212 56-57 -1.5613417013008188 1212 58-59 -0.5334210664564623 1212 60-61 -0.027736435495434364 1212 62-63 -4.576802593280291 1212 64-65 -3.8662462198946272 1212 66-67 -2.03018573044184 1212 68-69 0.08207689034306398 1212 70-71 -1.0382096312329558 1212 72-73 -1.3838779346575762 1212 74-75 -0.3854908921990443 1212 76-77 0.6488243137630398 1212 78-79 -1.1742101505120388 1212 80-81 0.4337927526114136 1212 82-83 0.5880008337845766 1212 84-85 -3.1154782957261737 1212 86-87 -2.1160982832956563 1212 88-89 -1.0323361118789904 1212 90-91 -0.08773864110223073 1212 92-93 -2.590715640823113 1212 94-95 -2.319454692496638 1212 96-97 -0.30208676708792837 1212 98-99 -0.648436325598059 1212 100-101 -0.17668447254282071 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 4.0 10 17.0 11 17.0 12 49.0 13 213.0 14 670.0 15 1592.0 16 3083.0 17 5175.0 18 8148.0 19 12032.0 20 16971.0 21 23537.0 22 31046.0 23 40439.0 24 51608.0 25 65224.0 26 80385.0 27 98090.0 28 118657.0 29 142877.0 30 169213.0 31 198820.0 32 232165.0 33 268861.0 34 303422.0 35 337961.0 36 363345.0 37 339448.0 38 130903.0 39 452.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.140537802101296 34.29020321034692 16.89983689830813 19.66942208924366 2 23.095108189544312 28.962304715022963 27.380151908480443 20.562435186952282 3 20.003435791752917 27.17320397763516 31.769905748940193 21.053454481671725 4 22.799106037205092 26.13793357162706 29.685273592162982 21.37768679900487 5 21.276089063722008 28.983023734844025 28.08787991960392 21.653007281830046 6 21.170322034326986 28.53689709097968 28.25733709496073 22.035443779732606 7 21.97871717732105 29.196068751196858 28.789161310157873 20.036052761324218 8 20.317222255616574 27.710830313881722 30.03031440727598 21.94163302322572 9 21.55489358095957 27.698972581704208 28.831829437854807 21.914304399481413 10-11 21.68403118222247 28.11663738365216 28.28323687840109 21.916094555724282 12-13 21.680462865118233 28.02221652522725 28.28726939922775 22.010051210426774 14-15 21.612970456011997 28.332856779429555 28.109662033226947 21.9445107313315 16-17 21.702061056842233 28.367936108453236 28.12866142492681 21.801341409777727 18-19 22.117280719970942 28.283250306940328 27.703553601967574 21.895915371121152 20-21 21.814522671925676 28.253568981476313 27.908512146460453 22.02339620013756 22-23 21.605937669058907 28.029740900340194 28.300990629763827 22.06333080083707 24-25 21.83565248895769 27.869004577872946 28.2051597886893 22.09018314448006 26-27 21.821610437694844 28.144590098563704 28.081573314124462 21.952226149616987 28-29 22.0617048791119 28.083429164174404 28.023335754150125 21.83153020256357 30-31 22.11123800964845 27.986005248278246 27.784670153037013 22.118086589036295 32-33 21.832449915862657 27.972948604121562 28.069518500525714 22.125082979490067 34-35 22.047038736681813 27.939116293476573 28.041647902873017 21.972197066968597 36-37 22.06022676845265 28.013760881768558 27.994184127259413 21.93182822251938 38-39 21.9387424957143 27.97166757488355 28.14789121236936 21.941698717032796 40-41 22.116727097799565 27.976239454114467 27.936871761342108 21.97016168674386 42-43 21.909048894915202 27.903674484558177 28.140352848007193 22.046923772519428 44-45 21.855492018695145 27.8420208466158 28.213420784929315 22.089066349759744 46-47 21.95588922334518 28.0729732375829 28.101934261268845 21.869203277803077 48-49 22.074505998831963 27.847433309267494 28.07468468604591 22.003376005854633 50-51 22.010717291206948 27.94122767313116 27.994718873114337 22.053336162547556 52-53 21.886029907488172 27.979995542667137 28.208988144381454 21.924986405463237 54-55 21.934816534569983 27.75718779737357 28.199267432475978 22.10872823558047 56-57 21.90454057596245 27.976691496551435 28.1032360421202 22.015531885365917 58-59 21.919881610035134 27.911483050652414 28.2048934168104 21.96374192250205 60-61 22.087954627162574 27.79367138065873 27.969372382161012 22.14900161001768 62-63 21.926326030930372 27.836253352892832 28.179409575526886 22.058011040649905 64-65 22.00858080377328 27.858279270130755 28.114918323381303 22.018221602714664 66-67 22.070143414321524 27.84858151724301 28.043257355611313 22.03801771282415 68-69 22.00290709610663 27.866717034433485 28.12610555879479 22.0042703106651 70-71 22.13970958984205 27.93429720643232 27.97818228402777 21.947810919697865 72-73 21.951565746225803 27.83547567977432 28.176383557097427 22.03657501690245 74-75 21.908489738590465 27.72565455899978 28.26342925064484 22.102426451764916 76-77 21.944406426795773 28.050642140019296 28.159145971540276 21.84580546164465 78-79 22.10332691173838 27.86796478141121 27.99840034949243 22.03030795735798 80-81 22.01003237081395 27.826387072194958 28.062685628210343 22.100894928780757 82-83 21.978708549018343 27.87292271867163 28.13925884123092 22.009109891079103 84-85 21.987330601529532 27.672135584802177 28.133002240771127 22.207531572897167 86-87 21.876860087273815 27.85292220121332 28.232445703518678 22.037772007994185 88-89 21.994376836693064 27.870201439198684 28.148797349971783 21.986624374136472 90-91 22.08481021038198 27.77305312190603 27.909974141886316 22.232162525825668 92-93 21.876925876820096 27.743154883530853 28.20960307062786 22.17031616902119 94-95 22.002940234397457 27.782044859106925 28.04656739953515 22.16844750696047 96-97 22.017540920382565 27.832955879637932 28.018857573481924 22.13064562649758 98-99 21.916974113070303 27.75749488383232 28.15327001916705 22.172260983930332 100-101 22.05329964080134 27.723359213770838 27.980685269832634 22.242655875595187 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 6.5 2 7.0 3 7.0 4 10.5 5 28.5 6 52.5 7 83.0 8 125.0 9 189.0 10 259.5 11 365.5 12 544.5 13 791.0 14 1137.5 15 1616.5 16 2308.5 17 3286.5 18 4659.5 19 6545.5 20 9159.5 21 12474.5 22 16357.0 23 21066.0 24 26467.0 25 32725.5 26 39427.5 27 46206.5 28 53330.0 29 60504.5 30 67750.0 31 74406.0 32 80005.0 33 85545.0 34 90979.5 35 95506.0 36 99211.0 37 102054.0 38 103082.5 39 102170.5 40 99546.0 41 95405.0 42 90690.5 43 85536.5 44 80207.5 45 75863.0 46 72010.0 47 68670.5 48 65286.5 49 61611.5 50 58674.5 51 55713.0 52 53046.5 53 51385.5 54 50801.5 55 50747.5 56 51120.5 57 51805.5 58 52221.0 59 52405.0 60 51686.0 61 49955.5 62 48073.5 63 45726.0 64 42627.0 65 39403.0 66 35861.0 67 32174.5 68 28517.0 69 24525.5 70 20661.0 71 16928.5 72 13323.5 73 10075.5 74 7230.5 75 5028.5 76 3462.5 77 2229.5 78 1355.0 79 833.0 80 507.5 81 320.0 82 219.5 83 143.0 84 93.0 85 71.5 86 59.0 87 41.5 88 31.0 89 22.0 90 13.5 91 8.0 92 8.5 93 5.5 94 2.5 95 1.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7353107826201778 2 0.0030876089326497234 3 1.6423451769413422E-4 4 1.6423451769413422E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.19011787768272978 14-15 1.6423451769413425E-5 16-17 5.912442636988833E-4 18-19 0.11583460532967288 20-21 3.284690353882685E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0017244624357884094 42-43 0.0 44-45 0.0 46-47 0.022368741309941084 48-49 3.284690353882685E-5 50-51 3.284690353882685E-5 52-53 1.8065796946354766E-4 54-55 0.0021678956335625717 56-57 0.0014616872074777946 58-59 0.09699690615015567 60-61 0.001658768628710756 62-63 0.0013467230450919007 64-65 0.0024142474101037734 66-67 0.004450755429511038 68-69 0.00499272933790168 70-71 0.003678853196348607 72-73 0.040385267900987606 74-75 0.04677399063928943 76-77 0.16104836805086803 78-79 3.9416284246592215E-4 80-81 0.11727986908538127 82-83 0.005041999693209921 84-85 0.005337621825059363 86-87 0.007062084260847773 88-89 0.007653328524546655 90-91 0.023042102832487033 92-93 0.011792038370438839 94-95 0.014846800399549734 96-97 0.011332181720895262 98-99 0.013155184867300152 100-101 0.014091321618156717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3044427.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.499977281254 #Duplication Level Percentage of deduplicated Percentage of total 1 97.723284400531 94.30294724500764 2 1.7894990317432191 3.453732318160933 3 0.15581329229357163 0.45107937499341155 4 0.11051285791190953 0.4265795311114288 5 0.09113019510796742 0.43970308787775503 6 0.052142803364828905 0.301906760405213 7 0.02983501100714165 0.20153545190625924 8 0.027773641039052728 0.21441245834290332 9 0.013087095066974141 0.11366139389765541 >10 0.006861513186454758 0.07603952086448901 >50 0.0 0.0 >100 6.0158747862355176E-5 0.0184028574322955 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.854071061648054E-5 0.0 0.0 2 1.3138761415530737E-4 0.0 0.0 3 1.3138761415530737E-4 0.0 3.2846903538826844E-5 4 1.6423451769413422E-4 0.0 3.2846903538826844E-5 5 1.6423451769413422E-4 0.0 3.2846903538826844E-5 6 2.2992832477178793E-4 0.0 3.2846903538826844E-5 7 2.2992832477178793E-4 0.0 3.2846903538826844E-5 8 2.2992832477178793E-4 0.0 6.569380707765369E-5 9 2.2992832477178793E-4 0.0 6.569380707765369E-5 10-11 2.6277522831061475E-4 0.0 6.569380707765369E-5 12-13 2.6277522831061475E-4 0.0 6.569380707765369E-5 14-15 2.791986800800282E-4 0.0 8.211725884706711E-5 16-17 2.956221318494416E-4 0.0 9.854071061648054E-5 18-19 3.12045583618855E-4 0.0 1.1496416238589396E-4 20-21 3.613159389270953E-4 0.0 1.3138761415530737E-4 22-23 3.613159389270953E-4 0.0 1.3138761415530737E-4 24-25 3.613159389270953E-4 0.0 1.9708142123296107E-4 26-27 3.7773939069650877E-4 0.0 2.2992832477178793E-4 28-29 4.27009746004749E-4 0.0 2.6277522831061475E-4 30-31 4.5985664954357585E-4 3.2846903538826844E-5 2.791986800800282E-4 32-33 5.748208119294698E-4 3.2846903538826844E-5 3.12045583618855E-4 34-35 6.569380707765369E-4 3.2846903538826844E-5 3.2846903538826845E-4 36-37 8.047491367012577E-4 3.2846903538826844E-5 3.2846903538826845E-4 38-39 0.0010675243650118725 3.2846903538826844E-5 3.2846903538826845E-4 40-41 0.0013467230450919007 3.2846903538826844E-5 4.5985664954357585E-4 42-43 0.0016423451769413424 3.2846903538826844E-5 4.5985664954357585E-4 44-45 0.002085778374715505 3.2846903538826844E-5 4.927035530824027E-4 46-47 0.0029233744149555893 3.2846903538826844E-5 4.927035530824027E-4 48-49 0.003629582841040367 3.2846903538826844E-5 5.255504566212295E-4 50-51 0.004631413398974585 3.2846903538826844E-5 5.255504566212295E-4 52-53 0.006027406799374727 3.2846903538826844E-5 5.583973601600564E-4 54-55 0.007669751976316069 6.569380707765369E-5 5.583973601600564E-4 56-57 0.009624142736876265 6.569380707765369E-5 5.583973601600564E-4 58-59 0.012662481314217749 8.211725884706711E-5 5.912442636988833E-4 60-61 0.016472722124721662 1.3138761415530737E-4 6.076677154682967E-4 62-63 0.020529314711766777 1.3138761415530737E-4 6.405146190071236E-4 64-65 0.024635177654120134 1.3138761415530737E-4 6.569380707765369E-4 66-67 0.029939952575640673 1.3138761415530737E-4 6.569380707765369E-4 68-69 0.0368213788670249 1.3138761415530737E-4 6.569380707765369E-4 70-71 0.044409013584493895 1.4781106592472079E-4 6.897849743153638E-4 72-73 0.053064172666974774 1.6423451769413422E-4 7.719022331624309E-4 74-75 0.06167006139414741 1.6423451769413422E-4 7.883256849318443E-4 76-77 0.07280516169380971 1.6423451769413422E-4 8.211725884706712E-4 78-79 0.08669940189073347 1.9708142123296107E-4 8.211725884706712E-4 80-81 0.10313927711191631 1.9708142123296107E-4 8.211725884706712E-4 82-83 0.12184558867727818 1.9708142123296107E-4 8.211725884706712E-4 84-85 0.14180008257711552 1.9708142123296107E-4 8.211725884706712E-4 86-87 0.16607394429230854 1.9708142123296107E-4 8.54019492009498E-4 88-89 0.19287701757999126 1.9708142123296107E-4 8.54019492009498E-4 >>END_MODULE >>Kmer Content pass >>END_MODULE