FastQCFastQC Report
Thu 11 Sep 2014
NYCLib24_GGTAGC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib24_GGTAGC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4327174
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT21500.012.24950248-49
CCGTCTT22500.012.23247650-51
TATGCCG20200.011.8688846-47
CGTATGC20600.011.86887944-45
TCGTATG22650.011.4234742-43
ATGCCGT22450.011.31376646-47
CTCGTAT23100.011.20093442-43
GTATGCC24300.010.55011544-45
CGTCTTC28600.010.370150-51
TCTCGTA25300.010.31831940-41
TGCCGTC26850.09.54362548-49
ATCTCGT32150.08.19367640-41
CATCTCG38950.07.30983738-39
CTGCTTG36050.07.18685356-57
ACGGTAG49650.07.026270494-95
GTCTTCT43500.06.885236752-53
CGGTAGC49750.06.81956732-33
GCATCTC46000.06.653739538-39
TAGCATC45550.06.406940536-37
CACGGTA53950.06.378276394-95
Produced by FastQC (version 0.11.2)