##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib23_GAGTGG_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2321214 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.86770629506801 27.0 22.0 33.0 2.0 33.0 2 24.93025933843239 27.0 15.0 33.0 2.0 33.0 3 25.77955587033337 33.0 22.0 33.0 6.0 33.0 4 27.88170241950979 33.0 22.0 37.0 2.0 37.0 5 27.905390024357942 33.0 15.0 37.0 6.0 37.0 6 28.95453499763486 33.0 22.0 37.0 15.0 37.0 7 28.628898929611832 33.0 27.0 37.0 6.0 37.0 8 28.39482744805089 33.0 22.0 37.0 6.0 37.0 9 29.13077768788229 33.0 27.0 37.0 15.0 37.0 10-11 28.712502811029054 33.0 24.5 37.0 10.5 37.0 12-13 28.99697938234045 33.0 27.0 37.0 10.5 37.0 14-15 29.280447645068485 33.0 24.5 37.0 6.0 40.0 16-17 30.114283086350504 33.0 27.0 37.0 15.0 40.0 18-19 30.394858466302548 33.0 27.0 37.0 15.0 40.0 20-21 30.379467597558865 33.0 27.0 37.0 15.0 40.0 22-23 30.36235564665731 33.0 27.0 37.0 15.0 40.0 24-25 29.942939125819507 33.0 27.0 37.0 10.5 40.0 26-27 29.431842777098538 33.0 27.0 37.0 10.5 40.0 28-29 29.54468846905111 33.0 27.0 37.0 15.0 40.0 30-31 29.420919398211453 33.0 27.0 37.0 10.5 40.0 32-33 29.244009169339837 33.0 27.0 37.0 6.0 40.0 34-35 29.26501154137447 33.0 24.5 37.0 10.5 40.0 36-37 29.524058100631827 33.0 27.0 37.0 10.5 40.0 38-39 29.509579685457695 33.0 27.0 37.0 15.0 40.0 40-41 29.536696099541015 33.0 27.0 37.0 15.0 40.0 42-43 29.39131570807345 33.0 27.0 37.0 15.0 40.0 44-45 29.095548923968234 33.0 24.5 37.0 10.5 40.0 46-47 28.6869657429259 33.0 22.0 37.0 10.5 40.0 48-49 28.477701754340618 33.0 22.0 37.0 10.5 40.0 50-51 27.66100842059371 30.0 22.0 37.0 10.5 38.5 52-53 27.359641549637388 33.0 22.0 37.0 6.0 37.0 54-55 28.252302674376423 33.0 22.0 37.0 10.5 40.0 56-57 28.113597022937135 33.0 22.0 37.0 6.0 40.0 58-59 28.249243283902302 33.0 22.0 37.0 10.5 40.0 60-61 27.975297409028208 33.0 22.0 37.0 10.5 40.0 62-63 27.803999975874696 33.0 22.0 37.0 10.5 40.0 64-65 27.727134163416213 33.0 22.0 37.0 6.0 37.0 66-67 27.573575939142188 33.0 22.0 37.0 10.5 37.0 68-69 27.27709293499005 30.0 22.0 37.0 6.0 37.0 70-71 27.16625007431456 27.0 22.0 35.0 6.0 37.0 72-73 26.90682267985632 27.0 22.0 33.0 6.0 37.0 74-75 26.852847475502045 27.0 22.0 33.0 6.0 37.0 76-77 26.463202229523 27.0 22.0 33.0 6.0 37.0 78-79 25.91488785609599 27.0 22.0 33.0 6.0 37.0 80-81 25.778057301050225 27.0 22.0 33.0 6.0 37.0 82-83 25.3515686188348 27.0 18.5 33.0 6.0 37.0 84-85 25.435076860642752 27.0 18.5 33.0 6.0 37.0 86-87 25.142075224429973 27.0 15.0 33.0 6.0 37.0 88-89 24.778495649259398 27.0 15.0 33.0 6.0 37.0 90-91 24.62307396043622 27.0 15.0 33.0 6.0 37.0 92-93 24.501934117233482 27.0 15.0 33.0 6.0 37.0 94-95 24.254960335410694 27.0 15.0 33.0 6.0 37.0 96-97 24.114748360125347 27.0 15.0 33.0 6.0 37.0 98-99 23.85924369747899 27.0 15.0 33.0 6.0 37.0 100-101 23.34542398934351 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.252691669339512 2306 2 8.484673724565155 2306 3 7.465372823373386 2306 4 9.111584274297115 2306 5 8.906905703229384 2306 6 7.933091220679078 2306 7 8.189171754142649 2306 8 8.60600825413098 2306 9 7.565258825412702 2306 10-11 7.841218035836452 2306 12-13 7.387041244677253 2306 14-15 8.020894701509643 2306 16-17 6.703546829997386 2306 18-19 6.094506311001769 2306 20-21 5.878223799605074 2306 22-23 5.634649612027502 2306 24-25 5.677385851458368 2306 26-27 6.244920157889041 2306 28-29 5.24908473909851 2306 30-31 5.14952507802861 2306 32-33 4.991356688953566 2306 34-35 4.439493585654951 2306 36-37 4.571578985343269 2306 38-39 4.102887992722486 2306 40-41 3.437667253834615 2306 42-43 3.2347937387877685 2306 44-45 2.781729232819675 2306 46-47 3.0874282654322833 2306 48-49 2.831086614968463 2306 50-51 2.7262342652937583 2306 52-53 3.182283720246236 2306 54-55 2.7476399259534077 2306 56-57 3.092173939000528 2306 58-59 2.586578486393414 2306 60-61 2.4928966142188465 2306 62-63 2.108099940960887 2306 64-65 1.6197217290596555 2306 66-67 1.4984767913753636 2306 68-69 1.650592337014718 2306 70-71 1.8965288241168032 2306 72-73 1.067729887337432 2306 74-75 0.6841822480901563 2306 76-77 0.8490625264227951 2306 78-79 1.1599823535921843 2306 80-81 0.9839168394619406 2306 82-83 0.9183646150332905 2306 84-85 0.6320339196294711 2306 86-87 0.5114720952009719 2306 88-89 0.47464389752906655 2306 90-91 0.3464181136929234 2306 92-93 0.3180192829755484 2306 94-95 0.47689119896508103 2306 96-97 0.11845225298364781 2306 98-99 0.03631483345638742 2306 100-101 -0.11109199620307919 2307 1 8.268932391400305 2307 2 8.470065016475846 2307 3 7.609195936545426 2307 4 9.212443942173245 2307 5 9.085134350795663 2307 6 7.566342376187119 2307 7 8.173908885471597 2307 8 8.509944132844037 2307 9 7.460666527795983 2307 10-11 7.724974302679758 2307 12-13 7.312400694248925 2307 14-15 8.144458220577416 2307 16-17 6.828912678147816 2307 18-19 6.345227844854012 2307 20-21 6.3100892864240095 2307 22-23 6.0247679480959135 2307 24-25 6.090880265875491 2307 26-27 6.817206595913163 2307 28-29 6.0539529283760025 2307 30-31 5.834040141035793 2307 32-33 5.970314774498796 2307 34-35 5.660219899621914 2307 36-37 5.646913781567353 2307 38-39 5.002258861875312 2307 40-41 4.445912951731369 2307 42-43 4.277676449075173 2307 44-45 4.134313317672358 2307 46-47 4.490886132673069 2307 48-49 4.145084258785932 2307 50-51 4.000428797143627 2307 52-53 4.586037992238527 2307 54-55 4.157595361734259 2307 56-57 4.50997459692498 2307 58-59 4.126440879311318 2307 60-61 4.018588413499195 2307 62-63 3.7933640140702956 2307 64-65 3.7406926945675494 2307 66-67 3.2817618217121556 2307 68-69 3.388788088358421 2307 70-71 3.105500948896619 2307 72-73 2.5121715925210317 2307 74-75 2.268709355480894 2307 76-77 2.447048291466981 2307 78-79 2.796501865493539 2307 80-81 2.5873237062661154 2307 82-83 2.7265640173307553 2307 84-85 1.9219544643367144 2307 86-87 1.8767255541973746 2307 88-89 2.066592415317114 2307 90-91 1.959462933855221 2307 92-93 1.7005955766176193 2307 94-95 2.0264448039996346 2307 96-97 1.7073740548234255 2307 98-99 1.5812776464221656 2307 100-101 1.2298494982038086 1101 1 8.008091268092443 1101 2 7.818108342956588 1101 3 7.2482908126169505 1101 4 8.8377082841305 1101 5 8.645411104994494 1101 6 7.334604468425592 1101 7 7.087355027979648 1101 8 8.005061273373887 1101 9 7.505415179904123 1101 10-11 7.601503335688566 1101 12-13 7.291830119019107 1101 14-15 7.829018070854747 1101 16-17 6.871698644557199 1101 18-19 5.932878246312452 1101 20-21 5.76047455076597 1101 22-23 5.27998186532178 1101 24-25 5.917879121621972 1101 26-27 6.43289499046729 1101 28-29 5.918714574549536 1101 30-31 5.6354425303922895 1101 32-33 5.699986044038376 1101 34-35 5.456872764134538 1101 36-37 5.3726543463243495 1101 38-39 5.039334130483979 1101 40-41 4.46816664631265 1101 42-43 4.283435244311974 1101 44-45 4.210372527943491 1101 46-47 4.55708701704528 1101 48-49 4.254865589998843 1101 50-51 3.734629371209799 1101 52-53 4.584318024044894 1101 54-55 4.341993174076684 1101 56-57 4.557699374862221 1101 58-59 4.073304461018775 1101 60-61 4.143392144929315 1101 62-63 3.9017215254158586 1101 64-65 3.6110157037603656 1101 66-67 3.2279068509972895 1101 68-69 3.411495896434136 1101 70-71 3.380275492164916 1101 72-73 2.943704795267241 1101 74-75 2.4739118301715557 1101 76-77 2.6741580816225223 1101 78-79 3.156225984030449 1101 80-81 2.6434965661241527 1101 82-83 2.953091983662553 1101 84-85 2.198022014082653 1101 86-87 2.244329991573739 1101 88-89 2.2189463775373532 1101 90-91 2.0733363289911715 1101 92-93 1.8791530414172755 1101 94-95 2.197066579725945 1101 96-97 2.022353109165774 1101 98-99 1.995134168667228 1101 100-101 1.568677898964058 2201 1 6.870591290167216 2201 2 7.6614560952050255 2201 3 6.172031358481956 2201 4 8.258680771113738 2201 5 3.6362941646169844 2201 6 5.3439116639188065 2201 7 6.38476772715542 2201 8 7.592296246094367 2201 9 6.34976691247952 2201 10-11 3.7528853048172444 2201 12-13 6.128061140845663 2201 14-15 6.780241258589417 2201 16-17 5.749254761084529 2201 18-19 1.6327889464948377 2201 20-21 4.765281788937074 2201 22-23 4.944741147273547 2201 24-25 5.286105614686079 2201 26-27 5.901671743866565 2201 28-29 4.731577142895485 2201 30-31 4.917924245121462 2201 32-33 5.142982365890539 2201 34-35 4.800083829384633 2201 36-37 4.8452754068357216 2201 38-39 4.245069549114888 2201 40-41 3.910434461390796 2201 42-43 3.812144772667274 2201 44-45 3.5049976050825578 2201 46-47 4.048850673726626 2201 48-49 3.6943682698763602 2201 50-51 3.4144103502112912 2201 52-53 4.25632273725434 2201 54-55 3.8544611239581172 2201 56-57 4.14630168849472 2201 58-59 3.7368223950554267 2201 60-61 3.68870820172722 2201 62-63 3.4888807571352487 2201 64-65 3.368873018310193 2201 66-67 3.011316706942221 2201 68-69 3.252671196626533 2201 70-71 2.9717374602732463 2201 72-73 2.2758373655655824 2201 74-75 2.12500296170483 2201 76-77 2.43431572493931 2201 78-79 2.636489684370794 2201 80-81 2.331799251495834 2201 82-83 2.5656054958767704 2201 84-85 2.1585219243119056 2201 86-87 2.0149832184641454 2201 88-89 1.9761954879239774 2201 90-91 1.9381341682142228 2201 92-93 1.7946034992480833 2201 94-95 1.7953922326732226 2201 96-97 1.7358784701373757 2201 98-99 1.5768956178712124 2201 100-101 1.3168916769585515 2304 1 1.4875387780578038 2304 2 4.650258249830511 2304 3 5.67766956594599 2304 4 4.0455124234540705 2304 5 6.319019348828153 2304 6 6.187083014614384 2304 7 6.851667374582718 2304 8 6.126016207315292 2304 9 5.588437338688433 2304 10-11 6.37912644488107 2304 12-13 6.330252371799833 2304 14-15 6.517825534866301 2304 16-17 5.088461164357362 2304 18-19 4.995365669940366 2304 20-21 4.600999538146077 2304 22-23 4.189961499432037 2304 24-25 4.375027548922709 2304 26-27 5.335989909438901 2304 28-29 4.661543344414984 2304 30-31 4.607207574170246 2304 32-33 4.640245752649495 2304 34-35 4.294160097247392 2304 36-37 4.42420319834962 2304 38-39 3.8528905022223867 2304 40-41 3.307781957284117 2304 42-43 2.8577643013886913 2304 44-45 2.6137454326067555 2304 46-47 3.7272509885678495 2304 48-49 3.292474773796645 2304 50-51 2.8734185581408163 2304 52-53 3.689831958142964 2304 54-55 3.3878340068152895 2304 56-57 3.9678610835081507 2304 58-59 3.374165920748272 2304 60-61 3.288434530349427 2304 62-63 3.0560477974136333 2304 64-65 3.0501427555802394 2304 66-67 2.732517723249199 2304 68-69 2.9661579445115613 2304 70-71 2.9858412983582348 2304 72-73 2.585602304362265 2304 74-75 2.1395857322412155 2304 76-77 2.47773394891351 2304 78-79 2.4978239230720334 2304 80-81 2.2125069927634264 2304 82-83 2.4760694071163982 2304 84-85 1.9789139356130505 2304 86-87 1.8732897645898063 2304 88-89 2.0277245982200505 2304 90-91 2.0308442055425537 2304 92-93 1.8569192340402978 2304 94-95 2.1127326487610922 2304 96-97 2.0395248615026276 2304 98-99 1.8078969669752922 2304 100-101 1.5542826962625327 1102 1 6.938094989649507 1102 2 7.456781530789517 1102 3 6.061379066369888 1102 4 5.967169935576951 1102 5 6.511917708005651 1102 6 5.651172576223022 1102 7 6.731259756118803 1102 8 6.339001345294626 1102 9 4.75303318519358 1102 10-11 5.923016974424474 1102 12-13 5.738181848001663 1102 14-15 6.031140899854702 1102 16-17 5.2756985696856304 1102 18-19 5.189435585817552 1102 20-21 5.50371294402224 1102 22-23 5.178171691157317 1102 24-25 1.35157006942989 1102 26-27 5.3535665883805805 1102 28-29 4.800184699695148 1102 30-31 4.563006540072742 1102 32-33 4.941906455567718 1102 34-35 4.951460497089705 1102 36-37 4.837071129886073 1102 38-39 3.774772712680843 1102 40-41 3.951796948155149 1102 42-43 4.045775993600152 1102 44-45 -3.856777508060926 1102 46-47 1.135589299526874 1102 48-49 -0.9570169893705227 1102 50-51 2.4853262272648173 1102 52-53 -0.4326109206404354 1102 54-55 2.4517282168305456 1102 56-57 -0.9284398730309142 1102 58-59 2.78592243327666 1102 60-61 3.7934492014639503 1102 62-63 3.385657633548014 1102 64-65 2.977545560997914 1102 66-67 2.953229426314774 1102 68-69 3.223644071660015 1102 70-71 3.094788793978786 1102 72-73 2.686346308025133 1102 74-75 2.4783852959101083 1102 76-77 2.760606813320276 1102 78-79 -1.6189634740535865 1102 80-81 1.2842983084294453 1102 82-83 2.45720478868159 1102 84-85 2.0004947355097897 1102 86-87 2.203080803599871 1102 88-89 2.129846465841755 1102 90-91 1.9913776889229986 1102 92-93 2.0735975935201765 1102 94-95 2.34239206182173 1102 96-97 2.2342776166099867 1102 98-99 2.2650747558804873 1102 100-101 1.7194921051823648 2202 1 -20.57732745111938 2202 2 -7.65034289674662 2202 3 -0.9625597075882695 2202 4 1.2396762746462038 2202 5 0.6999411488048324 2202 6 1.310609210571414 2202 7 3.7007630364828046 2202 8 2.5101682101248315 2202 9 2.957797086164984 2202 10-11 1.855889616170142 2202 12-13 1.1548817171086192 2202 14-15 2.3597786538116416 2202 16-17 1.368376689648457 2202 18-19 1.3851879302375316 2202 20-21 0.7307333928920272 2202 22-23 1.060511950012689 2202 24-25 2.109033384443144 2202 26-27 3.125159705768205 2202 28-29 -1.4181220871433275 2202 30-31 0.5410437132033721 2202 32-33 1.3070882250166527 2202 34-35 1.2964282560981069 2202 36-37 2.8075157952679213 2202 38-39 2.256615027676162 2202 40-41 1.805300918536343 2202 42-43 0.44882016892140086 2202 44-45 0.5752937306497827 2202 46-47 -1.4900101219963453 2202 48-49 0.6119155119850888 2202 50-51 1.5447990936988774 2202 52-53 1.4984723754406275 2202 54-55 -1.0178024230862057 2202 56-57 0.8557703571541246 2202 58-59 1.5994628459638243 2202 60-61 1.7912104171802667 2202 62-63 -2.399745133074692 2202 64-65 -0.24712014236517632 2202 66-67 0.3752624914935758 2202 68-69 1.2146367504291895 2202 70-71 -2.52828149193936 2202 72-73 -4.70675395283352 2202 74-75 -1.4715534928871286 2202 76-77 -0.2115740552728731 2202 78-79 1.5232712047514454 2202 80-81 1.481276206238885 2202 82-83 -0.7341765812251317 2202 84-85 -0.40555735213228417 2202 86-87 0.5649989339253239 2202 88-89 1.0101700010545827 2202 90-91 1.3512725416791405 2202 92-93 1.152458984770437 2202 94-95 1.5662276423481636 2202 96-97 1.3754181297082582 2202 98-99 1.207778936976613 2202 100-101 0.9569203752362974 2305 1 5.589252859761448 2305 2 7.041062731787338 2305 3 6.653285324983269 2305 4 7.695561726025389 2305 5 7.701379098051994 2305 6 6.04456745880309 2305 7 2.5817737216675916 2305 8 5.854906598675704 2305 9 6.320318316788676 2305 10-11 4.5868659023696985 2305 12-13 5.163943878941513 2305 14-15 5.472557339340415 2305 16-17 4.8563381197862725 2305 18-19 4.250407152103815 2305 20-21 5.061014891254494 2305 22-23 5.069170461253279 2305 24-25 5.234191942265689 2305 26-27 5.964729646788278 2305 28-29 5.0391463109389285 2305 30-31 5.2625579493667445 2305 32-33 5.198535777313857 2305 34-35 5.054065009980533 2305 36-37 4.591613610215713 2305 38-39 4.4781151100904 2305 40-41 3.8132056978453583 2305 42-43 3.8100899572959754 2305 44-45 2.913359970195714 2305 46-47 3.823283831665357 2305 48-49 3.340090072502221 2305 50-51 2.382960109615574 2305 52-53 3.890225397893129 2305 54-55 4.100422051791789 2305 56-57 4.483185094252715 2305 58-59 4.253687829660141 2305 60-61 4.498889094825945 2305 62-63 3.7696868895139737 2305 64-65 3.934859089247489 2305 66-67 3.5757747138830176 2305 68-69 3.9327204044613957 2305 70-71 3.6474224331281846 2305 72-73 -1.147267536849455 2305 74-75 1.9470058346127104 2305 76-77 2.877243407945297 2305 78-79 3.6043712914655543 2305 80-81 3.2476481524331398 2305 82-83 3.3620771542896755 2305 84-85 2.8179251509672056 2305 86-87 2.6545946741843167 2305 88-89 2.856724906851529 2305 90-91 2.7047803423656838 2305 92-93 2.6858827946013797 2305 94-95 2.9573890739192628 2305 96-97 2.9414577315400017 2305 98-99 2.702529329340038 2305 100-101 2.308530498570992 1103 1 -20.57732745111938 1103 2 -7.7124073526298 1103 3 -0.9002481474934179 1103 4 2.7121835793633267 1103 5 4.430051343822903 1103 6 4.356099788145961 1103 7 3.5784657244580984 1103 8 4.034003365383292 1103 9 4.407331500072566 1103 10-11 4.724920368828464 1103 12-13 4.438590123431769 1103 14-15 3.8476727270962776 1103 16-17 2.5618147765066226 1103 18-19 2.9371065823991565 1103 20-21 2.021243933443003 1103 22-23 2.4335404985412126 1103 24-25 2.7761486779453577 1103 26-27 4.120386869947197 1103 28-29 3.508385518136947 1103 30-31 3.4874465726067356 1103 32-33 2.6942305462590106 1103 34-35 2.303595043028274 1103 36-37 2.6181117171689188 1103 38-39 2.2047843648043965 1103 40-41 2.522609799948121 1103 42-43 2.154478824776973 1103 44-45 2.435956667427128 1103 46-47 1.9203503970066258 1103 48-49 -1.0775757141328555 1103 50-51 -1.9641906508262608 1103 52-53 1.5926572431947505 1103 54-55 2.27404807083542 1103 56-57 3.168302043431961 1103 58-59 2.6001598006778295 1103 60-61 3.107827276784775 1103 62-63 2.9524308332683518 1103 64-65 2.620538115033895 1103 66-67 2.4117674926579724 1103 68-69 2.7337552055804615 1103 70-71 2.617412520238421 1103 72-73 2.1458657456779306 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42-43 -22.9248937123881 1209 44-45 -25.665785107524492 1209 46-47 -19.445423956484074 1209 48-49 -18.91078793398958 1209 50-51 -21.260819249671535 1209 52-53 -23.66819429122604 1209 54-55 -20.79567832924892 1209 56-57 -24.43067500937423 1209 58-59 -24.64009455375955 1209 60-61 -24.35719898807561 1209 62-63 -24.489510001677736 1209 64-65 -24.33716163614111 1209 66-67 -12.316532396860719 1209 68-69 -23.95963710849197 1209 70-71 -23.790660468426218 1209 72-73 -14.372433864141243 1209 74-75 -15.262580665659462 1209 76-77 -12.860439300064623 1209 78-79 -13.657136037015727 1209 80-81 -22.828129049639397 1209 82-83 -14.722084455355294 1209 84-85 -12.151394520007168 1209 86-87 -12.156564375028399 1209 88-89 -9.339994413323467 1209 90-91 -12.177675152483207 1209 92-93 -16.27083430787639 1209 94-95 -21.45465755820509 1209 96-97 -17.236833028726927 1209 98-99 -9.135232510139005 1209 100-101 -4.265218518585922 1212 1 -20.57732745111938 1212 2 -20.39391136147691 1212 3 -21.272103276053 1212 4 -23.23086314444093 1212 5 -23.39577116101536 1212 6 -24.419681245860122 1212 7 -23.973925760197695 1212 8 -23.63395350495124 1212 9 -24.559215361967798 1212 10-11 -24.251994201257244 1212 12-13 -24.508465447483356 1212 14-15 -24.783789810918652 1212 16-17 -25.79884322736399 1212 18-19 -20.321502374648365 1212 20-21 -26.130954020662607 1212 22-23 -26.31735803615605 1212 24-25 -22.540088067784634 1212 26-27 -25.006513876527784 1212 28-29 -19.834032747726415 1212 30-31 -14.775361464726796 1212 32-33 -25.04602380817837 1212 34-35 -21.217535876892256 1212 36-37 -25.479759446779102 1212 38-39 -25.729138775919964 1212 40-41 -20.31445729728567 1212 42-43 -16.60013505441808 1212 44-45 -13.868930585899328 1212 46-47 -16.92935488651146 1212 48-49 -19.438143855475015 1212 50-51 -11.711187956037456 1212 52-53 -4.553515392143471 1212 54-55 -3.2114266297828564 1212 56-57 -2.9267431614056854 1212 58-59 -2.174825877481698 1212 60-61 -13.310016812453066 1212 62-63 -10.815198075072232 1212 64-65 -15.250005672838359 1212 66-67 -11.483883269669771 1212 68-69 -7.0579333077056 1212 70-71 -14.245444216787948 1212 72-73 -10.849861436033887 1212 74-75 -6.93683522094247 1212 76-77 -17.771485701990258 1212 78-79 -8.687174386557523 1212 80-81 -6.799950805864821 1212 82-83 -4.277172399218092 1212 84-85 -3.8056124596372385 1212 86-87 -2.4343078716702635 1212 88-89 -2.438078976102993 1212 90-91 -2.7179702195694055 1212 92-93 -2.844143383764628 1212 94-95 -2.6335566407738966 1212 96-97 -12.155868068580872 1212 98-99 -9.401047871374349 1212 100-101 -5.531698112243969 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 396.0 3 822.0 4 429.0 5 2481.0 6 2828.0 7 2930.0 8 5675.0 9 10544.0 10 14146.0 11 19104.0 12 25790.0 13 33068.0 14 37881.0 15 41705.0 16 44823.0 17 50343.0 18 56306.0 19 61823.0 20 66776.0 21 73735.0 22 80698.0 23 85159.0 24 89441.0 25 91217.0 26 95021.0 27 96832.0 28 98326.0 29 99116.0 30 102821.0 31 107702.0 32 114894.0 33 126930.0 34 136228.0 35 142127.0 36 139995.0 37 119769.0 38 43195.0 39 138.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.24464830434897 25.73814218482027 19.120622804281055 23.896586706549705 2 26.939280606928477 29.34021769924916 20.109308131843523 23.61119356197884 3 20.184631248807943 25.728753113613045 27.519559221312544 26.56705641626646 4 24.18610212693978 25.41050939036281 26.273427300150388 24.129961182547017 5 23.53438568929568 26.23750506607294 25.9678977677121 24.26021147691928 6 23.772670947903645 26.76056146169295 24.86851383743438 24.598253752969022 7 24.50425279516282 27.04070906238733 25.960600880334784 22.494437262115067 8 23.137372992129105 26.129344610513964 26.624371671643342 24.108910725713585 9 23.56408186860231 25.30158098240141 26.900605525167197 24.233731623829076 10-11 24.410525153368035 25.56673452412575 25.676521724756462 24.346218597749754 12-13 24.313339330171363 25.755397154227772 25.48017863308437 24.45108488251649 14-15 24.407773498340994 25.910006575719933 25.373325771835276 24.308894154103793 16-17 24.529922143475893 25.93088865807518 25.326435741456127 24.212753456992797 18-19 24.68400490313686 26.030658587691512 25.05589047796707 24.229446031204567 20-21 24.714261991849884 25.862986060975796 25.070063643362733 24.352688303811586 22-23 24.45340153098213 25.75069832402235 25.41452993731076 24.381370207684764 24-25 24.40806418716725 25.8630639586916 25.45120596131963 24.277665892821517 26-27 24.340017964074548 25.868424611770646 25.387510429992005 24.404046994162798 28-29 24.60472013370411 25.86185515329611 25.29683784870998 24.2365868642898 30-31 24.635371029580373 25.799850637760912 25.129261286412547 24.43551704624617 32-33 24.399106383535766 25.87145687927813 25.25327379440209 24.476162942784008 34-35 24.56912658200613 25.75273040547394 25.33670939212061 24.34143362039932 36-37 24.55075528653997 25.89878332999504 25.241305515240924 24.309155868224064 38-39 24.516120018651407 25.802529989818566 25.39688811426955 24.28446187726048 40-41 24.6851360152297 25.757988948013843 25.218262568530374 24.338612468226085 42-43 24.577233149199984 25.863669140304413 25.209862213974354 24.349235496521242 44-45 24.508044901247 25.684524211897113 25.39570325123651 24.411727635619375 46-47 24.595106166875585 25.910739747966176 25.222321499678714 24.271832585479526 48-49 24.593472407787804 25.80792692563665 25.287623925654305 24.310976740921237 50-51 24.73155831540515 25.68058949360949 25.12586176658268 24.461990424402686 52-53 24.51985286441867 25.86441712632742 25.287819028620017 24.327910980633888 54-55 24.547500135280583 25.760118056682895 25.2993315715091 24.393050236527422 56-57 24.54212775339762 25.792313773415238 25.299971661398203 24.365586811788948 58-59 24.651571259523035 25.824389027540512 25.18792338264276 24.33611633029369 60-61 24.678084706028187 25.677144963473125 25.133224766079177 24.511545564419503 62-63 24.630426921391095 25.74071157104065 25.165982615131583 24.462878892436677 64-65 24.70205302503439 25.75269954151942 25.21124334788931 24.33400408555688 66-67 24.809849534239646 25.738121659913944 25.109563918710894 24.34246488713552 68-69 24.79285452863307 25.709569567231487 25.177232297718135 24.320343606417307 70-71 24.915599801467593 25.7479438802298 25.009711297163957 24.326745021138656 72-73 24.859690421005272 25.726480561682425 25.053524927169235 24.36030409014307 74-75 24.9191167401394 25.65377220893455 24.98061475590118 24.446496295024872 76-77 24.991616078263206 25.77944509039707 24.922034936829583 24.306903894510143 78-79 25.1017337631003 25.741671600068276 24.884997087736657 24.271597549094768 80-81 25.27397251396272 25.671283964922875 24.704835525761517 24.34990799535289 82-83 25.10642774667577 25.73663846344007 24.8824023728636 24.274531417020558 84-85 25.322188419710795 25.622078534006587 24.69343871554863 24.362294330733985 86-87 25.29214156651185 25.693062688935754 24.73304267953478 24.281753065017618 88-89 25.399426040646254 25.744073283295847 24.738200030353617 24.118300645704274 90-91 25.60368871835923 25.664507641603418 24.39830552141393 24.333498118623417 92-93 25.606339368803344 25.59313450812002 24.492341072920823 24.308185050155814 94-95 25.7680917608085 25.61526092878914 24.43157921154522 24.185068098857144 96-97 25.877100918676675 25.68113875526304 24.346369446937054 24.09539087912323 98-99 25.962359821449166 25.725171473337543 24.230867636443115 24.08160106877018 100-101 26.51781645385851 25.588054909662944 23.80946418629512 24.08466445018342 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 145.0 1 144.0 2 173.5 3 187.5 4 175.0 5 186.5 6 220.0 7 261.0 8 329.0 9 440.0 10 565.5 11 722.5 12 1018.0 13 1382.5 14 1724.5 15 2225.5 16 2849.5 17 3573.0 18 4544.0 19 5782.5 20 7208.5 21 8933.5 22 11117.0 23 13668.5 24 16480.5 25 19460.0 26 22703.0 27 26152.0 28 29656.0 29 33150.5 30 36595.5 31 39939.0 32 43115.0 33 46093.5 34 48791.5 35 51395.5 36 53557.5 37 55253.0 38 56161.5 39 56166.0 40 55149.5 41 53125.5 42 51245.5 43 49201.5 44 46993.0 45 45367.5 46 43822.5 47 42700.5 48 42261.0 49 41687.0 50 41759.0 51 42634.0 52 44112.0 53 46781.0 54 50527.0 55 54780.5 56 59112.5 57 63571.0 58 67062.0 59 69490.0 60 70198.0 61 68856.0 62 65861.5 63 61028.5 64 55376.0 65 48947.5 66 42298.0 67 35853.0 68 29711.0 69 24321.0 70 19885.5 71 16206.5 72 13102.0 73 10707.5 74 8753.0 75 7060.5 76 5733.5 77 4656.5 78 3792.0 79 3119.5 80 2506.5 81 2024.5 82 1626.0 83 1278.5 84 1043.5 85 835.0 86 648.0 87 503.5 88 414.0 89 326.5 90 259.0 91 214.0 92 155.5 93 119.5 94 82.5 95 58.5 96 41.0 97 26.5 98 14.0 99 7.5 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.412679744306214 2 10.73890645153786 3 9.42407722855368 4 11.368706202874874 5 8.902970600728755 6 5.121931885642599 7 9.427480620054851 8 9.726505182202072 9 5.204604142487509 10-11 6.176272416071934 12-13 5.172034978248451 14-15 7.132323861565543 16-17 2.5490325321146607 18-19 2.945463020643508 20-21 2.9715485086683087 22-23 3.0204022550268954 24-25 3.364037094382508 26-27 4.121162460677904 28-29 1.6870482428591245 30-31 2.134895791598707 32-33 2.9154787107091376 34-35 1.8871590469469857 36-37 2.889414763136876 38-39 1.3255994492537095 40-41 1.10713617960257 42-43 0.5255439610479689 44-45 1.2179402674634912 46-47 1.2776288614492244 48-49 1.1869004753547066 50-51 1.4982246359017306 52-53 2.3989386588224955 54-55 1.6767303660929151 56-57 1.717786468632362 58-59 1.5211436774032898 60-61 1.2993631780611352 62-63 0.5823461343934683 64-65 1.1963136531142755 66-67 0.5392006079577324 68-69 0.7703296637018388 70-71 0.9626428239705602 72-73 0.08476168074119836 74-75 0.10511740839060939 76-77 0.4412777107151689 78-79 0.8134105687799573 80-81 0.28678958510503555 82-83 0.49245782594797377 84-85 0.06595686567459959 86-87 0.09925840529998527 88-89 0.08125058697733169 90-91 0.0876481013814323 92-93 0.16739085668102982 94-95 0.43778815740384125 96-97 0.3561713827333456 98-99 0.06627997246268548 100-101 0.21895870005953783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2321214.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.39050607954415 #Duplication Level Percentage of deduplicated Percentage of total 1 98.68139396836129 97.09312293181942 2 1.1007568367807454 2.1660804448275144 3 0.1487205105291894 0.4389805888612535 4 0.04469310058339444 0.17589507138656593 5 0.020466347998654714 0.10068471685938513 6 0.0021355838166086014 0.01260727034948428 7 0.0018336519301030301 0.012628975896359902 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.7232362031247442E-4 8.616181015623721E-5 0.0 2 2.1540452539059302E-4 8.616181015623721E-5 0.0 3 2.5848543046871165E-4 8.616181015623721E-5 0.0 4 2.5848543046871165E-4 8.616181015623721E-5 0.0 5 2.5848543046871165E-4 8.616181015623721E-5 0.0 6 3.0156633554683025E-4 8.616181015623721E-5 0.0 7 3.4464724062494885E-4 8.616181015623721E-5 0.0 8 4.7388995585930465E-4 8.616181015623721E-5 0.0 9 6.031326710936605E-4 8.616181015623721E-5 4.3080905078118606E-5 10-11 6.031326710936605E-4 8.616181015623721E-5 4.3080905078118606E-5 12-13 6.462135761717791E-4 8.616181015623721E-5 4.3080905078118606E-5 14-15 6.677540287108384E-4 8.616181015623721E-5 4.3080905078118606E-5 16-17 7.754562914061349E-4 8.616181015623721E-5 4.3080905078118606E-5 18-19 8.400776490233128E-4 8.616181015623721E-5 6.462135761717791E-5 20-21 9.046990066404907E-4 1.2924271523435583E-4 8.616181015623721E-5 22-23 0.0010985630794920244 1.2924271523435583E-4 8.616181015623721E-5 24-25 0.0012708866998044988 1.2924271523435583E-4 1.0770226269529653E-4 26-27 0.0014001294150388548 1.2924271523435583E-4 1.7232362031247442E-4 28-29 0.0015293721302732104 1.2924271523435583E-4 1.7232362031247442E-4 30-31 0.0021971261589840488 1.2924271523435583E-4 1.9386407285153372E-4 32-33 0.0034033915011713696 1.2924271523435583E-4 2.1540452539059302E-4 34-35 0.005277410872069529 1.2924271523435583E-4 2.1540452539059302E-4 36-37 0.007646860651366052 1.2924271523435583E-4 2.1540452539059302E-4 38-39 0.012407300662498158 1.7232362031247442E-4 2.1540452539059302E-4 40-41 0.017770873344723923 1.7232362031247442E-4 2.3694497792965235E-4 42-43 0.025503895806246214 1.7232362031247442E-4 2.8002588300777095E-4 44-45 0.03575715121483844 1.7232362031247442E-4 3.2310678808588955E-4 46-47 0.05051236120409407 1.7232362031247442E-4 3.4464724062494885E-4 48-49 0.06847709862166952 1.9386407285153372E-4 3.6618769316400815E-4 50-51 0.09318399768397054 2.1540452539059302E-4 3.8772814570306745E-4 52-53 0.12097118145935704 2.1540452539059302E-4 3.8772814570306745E-4 54-55 0.15394961429665682 2.1540452539059302E-4 3.8772814570306745E-4 56-57 0.19227007936364332 2.1540452539059302E-4 3.8772814570306745E-4 58-59 0.2428901428304327 2.1540452539059302E-4 3.8772814570306745E-4 60-61 0.3021263873128458 2.1540452539059302E-4 3.8772814570306745E-4 62-63 0.3719174535393979 2.1540452539059302E-4 4.3080905078118605E-4 64-65 0.45041086259173 2.1540452539059302E-4 4.3080905078118605E-4 66-67 0.5397391192712089 2.1540452539059302E-4 4.3080905078118605E-4 68-69 0.6415392979708032 2.1540452539059302E-4 4.7388995585930465E-4 70-71 0.7576423371563328 2.1540452539059302E-4 4.7388995585930465E-4 72-73 0.8743915899180343 2.1540452539059302E-4 4.7388995585930465E-4 74-75 1.0076623697771943 2.1540452539059302E-4 4.7388995585930465E-4 76-77 1.1551498483121332 2.1540452539059302E-4 4.7388995585930465E-4 78-79 1.317090970500781 2.1540452539059302E-4 4.7388995585930465E-4 80-81 1.5109335028997757 2.1540452539059302E-4 4.7388995585930465E-4 82-83 1.7141030512481832 2.1540452539059302E-4 4.7388995585930465E-4 84-85 1.925134864773347 2.1540452539059302E-4 4.7388995585930465E-4 86-87 2.142714975870385 2.1540452539059302E-4 4.7388995585930465E-4 88-89 2.384226529738318 2.1540452539059302E-4 4.7388995585930465E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTACG 350 0.0052164705 11.26204 1 CTAAGAG 625 0.008821422 5.090099 24-25 >>END_MODULE