##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib23_GAGTGG_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2321214 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.248852970902295 33.0 33.0 33.0 27.0 33.0 2 31.661216501365235 33.0 33.0 33.0 27.0 33.0 3 31.810590492733542 33.0 33.0 33.0 27.0 33.0 4 35.638117381680445 37.0 37.0 37.0 33.0 37.0 5 35.15294367516308 37.0 37.0 37.0 33.0 37.0 6 34.93130835847104 37.0 33.0 37.0 33.0 37.0 7 34.7237915159912 37.0 33.0 37.0 27.0 37.0 8 34.77373736329352 37.0 33.0 37.0 27.0 37.0 9 34.678749998922974 37.0 33.0 37.0 27.0 37.0 10-11 34.52292033392871 37.0 33.0 37.0 27.0 37.0 12-13 34.53327310622804 37.0 33.0 37.0 27.0 37.0 14-15 35.44207169179576 37.0 33.0 40.0 27.0 40.0 16-17 35.33326483469426 37.0 33.0 40.0 27.0 40.0 18-19 35.53865843476733 37.0 33.0 40.0 27.0 40.0 20-21 35.196352210524324 37.0 33.0 40.0 27.0 40.0 22-23 35.22264319446634 37.0 33.0 40.0 27.0 40.0 24-25 35.05867899297523 37.0 33.0 40.0 27.0 40.0 26-27 34.63971805270863 37.0 33.0 40.0 27.0 40.0 28-29 34.25739246790688 37.0 33.0 40.0 27.0 40.0 30-31 34.14168448062092 37.0 33.0 38.5 24.5 40.0 32-33 34.1503758378159 37.0 33.0 37.0 24.5 40.0 34-35 33.98083696720768 37.0 33.0 37.0 24.5 40.0 36-37 33.65500466566202 37.0 33.0 37.0 22.0 40.0 38-39 33.5150983493982 37.0 33.0 37.0 22.0 40.0 40-41 33.16967263681849 37.0 33.0 37.0 22.0 40.0 42-43 32.74302024716377 37.0 33.0 37.0 22.0 40.0 44-45 32.57630339124269 37.0 33.0 37.0 22.0 40.0 46-47 32.38332463099051 37.0 30.0 37.0 22.0 40.0 48-49 32.36766342956746 37.0 33.0 37.0 22.0 40.0 50-51 32.64849557171377 37.0 33.0 37.0 22.0 40.0 52-53 32.46792475833766 37.0 33.0 37.0 22.0 40.0 54-55 32.07552987359201 33.0 27.0 37.0 22.0 40.0 56-57 32.07752775056501 33.0 27.0 37.0 22.0 40.0 58-59 31.746076406570012 33.0 27.0 37.0 22.0 40.0 60-61 31.6282895502095 33.0 27.0 37.0 22.0 40.0 62-63 31.605506428963466 33.0 27.0 37.0 22.0 40.0 64-65 31.239165798586427 33.0 27.0 37.0 15.0 40.0 66-67 30.88724671658882 33.0 27.0 37.0 15.0 38.5 68-69 30.721709846657827 33.0 27.0 37.0 15.0 37.0 70-71 30.563311913507327 33.0 27.0 37.0 15.0 37.0 72-73 30.353525353543446 33.0 27.0 37.0 15.0 37.0 74-75 30.009379143844555 33.0 27.0 37.0 15.0 37.0 76-77 28.924580844334045 33.0 27.0 33.0 15.0 37.0 78-79 29.89079464452653 33.0 27.0 37.0 15.0 37.0 80-81 29.645420025900243 33.0 27.0 37.0 15.0 37.0 82-83 29.63342500949934 33.0 27.0 37.0 15.0 37.0 84-85 29.53178487636211 33.0 27.0 37.0 15.0 37.0 86-87 29.3361284224548 33.0 27.0 37.0 15.0 37.0 88-89 29.205702920971525 33.0 27.0 35.0 15.0 37.0 90-91 29.11031425797018 33.0 27.0 33.0 15.0 37.0 92-93 28.902138062238123 33.0 27.0 33.0 15.0 37.0 94-95 28.79342102882371 33.0 27.0 33.0 15.0 37.0 96-97 28.649338665026143 33.0 27.0 33.0 15.0 37.0 98-99 28.38358763991601 33.0 27.0 33.0 15.0 37.0 100-101 27.55010072315607 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.0418322514465643 2306 2 0.5461593613715792 2306 3 0.526403243481905 2306 4 0.3443568761610081 2306 5 0.6286798509117588 2306 6 0.668128752406524 2306 7 0.8017198644330961 2306 8 0.7771760237918244 2306 9 0.9669023032753827 2306 10-11 1.1464192431739946 2306 12-13 1.3739669153408656 2306 14-15 1.431263728655452 2306 16-17 1.4204785449319104 2306 18-19 1.2125563529349463 2306 20-21 1.5335960460741092 2306 22-23 1.153071251459643 2306 24-25 1.092192726328392 2306 26-27 1.2906896235753251 2306 28-29 1.5663977317293458 2306 30-31 1.4946406716018217 2306 32-33 1.108804799880751 2306 34-35 0.9972410293797793 2306 36-37 1.0695230034583219 2306 38-39 0.9485748307344366 2306 40-41 1.1100128945012742 2306 42-43 1.0502651634310993 2306 44-45 0.7835401536697262 2306 46-47 0.6514441396830222 2306 48-49 0.580800924510875 2306 50-51 0.7109570123179054 2306 52-53 0.8739500019993187 2306 54-55 1.2366103324653572 2306 56-57 0.8003818669030061 2306 58-59 0.8590729128983483 2306 60-61 0.6557661576119145 2306 62-63 0.31353986327135175 2306 64-65 0.37234617301473705 2306 66-67 0.6219030070844767 2306 68-69 0.33940705442889296 2306 70-71 0.308107180790266 2306 72-73 0.18919397666699922 2306 74-75 0.39900923052526593 2306 76-77 0.43297754542250644 2306 78-79 0.41074952814296495 2306 80-81 0.855978564255409 2306 82-83 0.8172575465799738 2306 84-85 0.4675769720990033 2306 86-87 0.2651333751262577 2306 88-89 0.11910763819822279 2306 90-91 -0.2333111234764118 2306 92-93 -0.19474224563351328 2306 94-95 -0.3299414836165475 2306 96-97 -0.354427658460736 2306 98-99 -0.12757591360777454 2306 100-101 -0.2719797856050903 2307 1 0.5884150340002741 2307 2 0.44029449935505127 2307 3 0.3424610414674838 2307 4 0.3507407758312908 2307 5 0.5313653720571807 2307 6 0.5514186757741371 2307 7 0.7135884752640109 2307 8 0.6503494177485507 2307 9 0.8960467003787045 2307 10-11 1.09702240331913 2307 12-13 1.32990931307652 2307 14-15 1.2809884015751578 2307 16-17 1.374351943405287 2307 18-19 1.2892977073258791 2307 20-21 1.4568676770342037 2307 22-23 1.2653550171361587 2307 24-25 1.1960013862401695 2307 26-27 1.2818157357988653 2307 28-29 1.587087724352152 2307 30-31 1.6968602256585257 2307 32-33 1.3248595867704438 2307 34-35 1.3192280515565002 2307 36-37 1.351780685516708 2307 38-39 0.9369151146807795 2307 40-41 1.1267370856456438 2307 42-43 1.2235936288260802 2307 44-45 1.0577111613329677 2307 46-47 1.0184472335840482 2307 48-49 1.0762731772622658 2307 50-51 1.0076020869178137 2307 52-53 1.2312769392631324 2307 54-55 1.5109966334846128 2307 56-57 1.241674906754863 2307 58-59 1.1980125931199694 2307 60-61 1.044131847679811 2307 62-63 0.8079766754241362 2307 64-65 0.9022183143555829 2307 66-67 1.0028764567464812 2307 68-69 0.9074273341596992 2307 70-71 0.8548924595424658 2307 72-73 0.7806251505427291 2307 74-75 0.8647457822182574 2307 76-77 0.9450682245751842 2307 78-79 0.944727298727571 2307 80-81 1.5222915526175207 2307 82-83 1.2407832387556432 2307 84-85 1.0850309776093106 2307 86-87 1.0985364027831785 2307 88-89 0.8444525968709335 2307 90-91 0.6155597155654569 2307 92-93 0.6801235724059609 2307 94-95 0.5137931786137706 2307 96-97 0.41606454280091043 2307 98-99 0.5385067259188823 2307 100-101 0.5973064717532743 1101 1 1.1080382061037604 1101 2 0.6012358903018722 1101 3 0.36967274776281656 1101 4 0.3768582654554322 1101 5 0.5204559814206675 1101 6 0.6201188813518854 1101 7 0.8031193313207936 1101 8 0.5546692656985925 1101 9 0.8449361926404535 1101 10-11 0.9022812179500619 1101 12-13 1.2825535084141748 1101 14-15 1.3447096497812083 1101 16-17 1.3114340969291973 1101 18-19 0.9904133545079148 1101 20-21 1.341161342912173 1101 22-23 0.9638426630769672 1101 24-25 1.0669006919303996 1101 26-27 1.1845519562713136 1101 28-29 1.3454667631791963 1101 30-31 1.3169922996665164 1101 32-33 0.8556902372494761 1101 34-35 1.0503478684759386 1101 36-37 1.0333354157443964 1101 38-39 0.8484751782935191 1101 40-41 1.036855098911225 1101 42-43 1.0381880976209246 1101 44-45 0.9540948353564715 1101 46-47 0.8231758127702733 1101 48-49 0.6860453932352684 1101 50-51 0.7750369226495479 1101 52-53 1.0295220616400726 1101 54-55 1.3969509215261944 1101 56-57 1.1630408351517438 1101 58-59 1.0262270727755265 1101 60-61 0.7990917988066109 1101 62-63 0.6439990368530957 1101 64-65 0.5287387169776672 1101 66-67 0.6202754133424229 1101 68-69 0.5737507894174456 1101 70-71 0.698653950562985 1101 72-73 0.5123636401280862 1101 74-75 0.5886262562107305 1101 76-77 0.759056626886963 1101 78-79 0.7450439078583244 1101 80-81 1.4902115324476277 1101 82-83 1.1520807058825966 1101 84-85 0.8591280840054942 1101 86-87 0.7208628253424259 1101 88-89 0.7606270082591635 1101 90-91 0.5071465430740858 1101 92-93 0.6326427725921455 1101 94-95 0.7119654760421064 1101 96-97 0.5700262781874379 1101 98-99 0.5927833249646888 1101 100-101 0.7237095399042381 2201 1 1.4081389259376031 2201 2 0.6750236761824517 2201 3 0.21622583563120656 2201 4 0.12501798478204051 2201 5 0.22268007863601014 2201 6 0.44118402351833197 2201 7 0.5797815274370066 2201 8 0.587771065724688 2201 9 0.614040574924509 2201 10-11 0.7613171880578733 2201 12-13 1.0283155345055732 2201 14-15 0.9657816596550077 2201 16-17 1.039351896358781 2201 18-19 0.8288536785459613 2201 20-21 1.1796970299723313 2201 22-23 0.9698427042832165 2201 24-25 0.7748512012690085 2201 26-27 1.022516505165953 2201 28-29 1.336904243005307 2201 30-31 1.37276328321051 2201 32-33 0.9078098962579944 2201 34-35 0.7242888434745396 2201 36-37 0.8376609921657874 2201 38-39 0.48817470628579684 2201 40-41 0.9228568276112767 2201 42-43 0.8343663362109695 2201 44-45 0.6862585261801328 2201 46-47 0.8021299590504753 2201 48-49 0.8702403458628041 2201 50-51 0.7129267508881867 2201 52-53 0.9405597820321603 2201 54-55 1.2640483242573843 2201 56-57 0.9535447777063091 2201 58-59 0.9547590807067436 2201 60-61 0.7643422984323216 2201 62-63 0.622973511547066 2201 64-65 0.6832727479392524 2201 66-67 0.7071059958319275 2201 68-69 0.8602614544764187 2201 70-71 0.7103907446975697 2201 72-73 0.5210074347129954 2201 74-75 0.648789236717235 2201 76-77 0.774264693892242 2201 78-79 0.5955458882107934 2201 80-81 1.1964974861219986 2201 82-83 1.038940741025634 2201 84-85 0.9183719886699429 2201 86-87 0.8200097776010686 2201 88-89 0.9135035082400336 2201 90-91 0.7454296947238603 2201 92-93 0.8320877833499196 2201 94-95 0.7349738261931478 2201 96-97 0.6357017711661896 2201 98-99 0.6371233628155686 2201 100-101 0.8446571461648418 2304 1 -1.7871056606302105 2304 2 -0.24732230914459308 2304 3 -0.16909172159261132 2304 4 -0.007274443556042343 2304 5 0.3150985339455161 2304 6 0.4291905797278375 2304 7 0.4142774019573636 2304 8 0.6322358029751314 2304 9 0.7549384555170917 2304 10-11 0.7439292884125592 2304 12-13 1.0861570524271187 2304 14-15 1.1696206142888599 2304 16-17 1.23927252753721 2304 18-19 0.8569662971178715 2304 20-21 1.0206544585451667 2304 22-23 0.7002177087696708 2304 24-25 0.9778300379590519 2304 26-27 1.0744409012485931 2304 28-29 1.3549781558927378 2304 30-31 1.3983931943731207 2304 32-33 0.9337937725590493 2304 34-35 0.794552293270101 2304 36-37 1.013633593258362 2304 38-39 0.7265954240767343 2304 40-41 0.8259844136077419 2304 42-43 0.6962754446591717 2304 44-45 0.8031179689184071 2304 46-47 0.7445324943487108 2304 48-49 0.7607298960116466 2304 50-51 0.6069782967378714 2304 52-53 0.824769498497119 2304 54-55 1.069615371734205 2304 56-57 0.6823651053740285 2304 58-59 0.6420926230897166 2304 60-61 0.42353713292905226 2304 62-63 0.11713747268313668 2304 64-65 0.2102842617217 2304 66-67 0.16714045886105922 2304 68-69 0.31547057566263703 2304 70-71 0.1525520166737948 2304 72-73 0.13322127037404385 2304 74-75 0.3547875470948192 2304 76-77 0.3226601708999155 2304 78-79 0.4243786221266319 2304 80-81 1.1566064218277745 2304 82-83 0.8202952962148906 2304 84-85 0.5571343156196207 2304 86-87 0.3266672779873794 2304 88-89 0.12977233715957937 2304 90-91 0.10490455452772451 2304 92-93 -0.0021043944603711395 2304 94-95 -0.0311391907057903 2304 96-97 -0.08311302797977049 2304 98-99 0.411691051818071 2304 100-101 0.3757036564249816 1102 1 1.3965994169090514 1102 2 0.05022748046374659 1102 3 0.2427847842472879 1102 4 0.19204759141318561 1102 5 0.24892078407904705 1102 6 0.12281104251952968 1102 7 -5.153659121524434 1102 8 -1.3227182452844062 1102 9 0.21000239892040895 1102 10-11 0.6235410200689273 1102 12-13 0.9047457257354878 1102 14-15 0.8431034125396479 1102 16-17 0.590615200227731 1102 18-19 0.7486011103649588 1102 20-21 1.0286805838026183 1102 22-23 0.6188342620472653 1102 24-25 0.9398221142258336 1102 26-27 1.0225001011081432 1102 28-29 0.9012502170043675 1102 30-31 1.3105029171146754 1102 32-33 0.9444225736165706 1102 34-35 1.0618047589718742 1102 36-37 1.1614139031626607 1102 38-39 0.8726280175773056 1102 40-41 1.2568418642615526 1102 42-43 1.2045305557424015 1102 44-45 1.167598075753645 1102 46-47 1.0322607721744674 1102 48-49 0.8927667675248117 1102 50-51 0.6406805648427607 1102 52-53 0.9721558670312618 1102 54-55 1.4413549328802482 1102 56-57 1.1171038065397987 1102 58-59 1.033270592972137 1102 60-61 0.850773854515456 1102 62-63 0.6115292662463503 1102 64-65 0.4390834124375864 1102 66-67 0.47045004025082093 1102 68-69 0.6797773776304616 1102 70-71 0.5311230164962026 1102 72-73 0.4401529911898301 1102 74-75 0.46477724132230236 1102 76-77 0.6091107766433019 1102 78-79 0.6534763175555156 1102 80-81 1.2225822083396451 1102 82-83 1.0336887048079717 1102 84-85 0.776691499117657 1102 86-87 0.6007177286590171 1102 88-89 0.46919973607240806 1102 90-91 0.15126417070257503 1102 92-93 0.3256610874975294 1102 94-95 0.300627873561659 1102 96-97 0.14718296454826074 1102 98-99 0.2218311128403414 1102 100-101 0.33321688502470437 2202 1 1.4890142977852499 2202 2 0.9768988709040407 2202 3 0.32036615032057725 2202 4 -0.4013880944707111 2202 5 -0.017733145711083864 2202 6 0.36870448185335647 2202 7 0.6370033258973109 2202 8 -4.146329446563534 2202 9 -0.5285860089143739 2202 10-11 0.3757847507124268 2202 12-13 -0.01141783430806953 2202 14-15 0.14984125114116864 2202 16-17 0.2874183987913028 2202 18-19 0.7700555132744071 2202 20-21 1.1130430072963406 2202 22-23 -2.8213206888570213 2202 24-25 -3.0136818561553653 2202 26-27 -1.4434425112252356 2202 28-29 0.4916994144884015 2202 30-31 1.1702739872163406 2202 32-33 1.1440591707436099 2202 34-35 -2.211369524161473 2202 36-37 -0.3998527915745953 2202 38-39 0.19584335549715348 2202 40-41 1.3057779640939273 2202 42-43 1.3929187287487537 2202 44-45 1.3255157757457923 2202 46-47 0.6306959294161558 2202 48-49 0.9094794971672968 2202 50-51 -3.896155904173554 2202 52-53 -0.45017787499504536 2202 54-55 1.3481688217639807 2202 56-57 1.373653157917449 2202 58-59 1.4392922295865702 2202 60-61 1.4088824221671281 2202 62-63 1.058281208156295 2202 64-65 1.07640246068377 2202 66-67 1.2667252767331938 2202 68-69 1.101015129979828 2202 70-71 1.3434797652786301 2202 72-73 1.2726972255582503 2202 74-75 0.7195639882931175 2202 76-77 1.2649588269428342 2202 78-79 1.0706719355753513 2202 80-81 1.683650337644913 2202 82-83 1.591558115962382 2202 84-85 1.4980447489688054 2202 86-87 1.499596481632242 2202 88-89 1.4584070571795742 2202 90-91 1.1697292410552684 2202 92-93 1.304117402793345 2202 94-95 1.3657300993617447 2202 96-97 1.2414453108169425 2202 98-99 1.3008707276178377 2202 100-101 1.2912766692026594 2305 1 1.3776311002562416 2305 2 0.5096531546526712 2305 3 0.18320091336173405 2305 4 -0.1857132652115041 2305 5 0.2611326194155126 2305 6 0.2911763356003334 2305 7 0.5988369351391682 2305 8 -0.07807863496247336 2305 9 0.41828977491667274 2305 10-11 0.22007684826222373 2305 12-13 0.7672945686760428 2305 14-15 0.565579503529392 2305 16-17 0.687650054457464 2305 18-19 0.6715531232615817 2305 20-21 0.8271646865401365 2305 22-23 0.4807052680439128 2305 24-25 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-0.9722438186061666 1208 42-43 -0.064336757275953 1208 44-45 -0.32637085287980483 1208 46-47 -0.26225217169708515 1208 48-49 -1.4974743366655545 1208 50-51 -0.32078473815963093 1208 52-53 -1.5811214855460278 1208 54-55 -0.8656928779077013 1208 56-57 -6.668868462362401 1208 58-59 -2.1051200032116952 1208 60-61 -1.87000682765564 1208 62-63 0.018494989973813603 1208 64-65 -0.1592695198830114 1208 66-67 0.7050723902496223 1208 68-69 0.38941566852041376 1208 70-71 -4.246280404373916 1208 72-73 -0.7828831867644404 1208 74-75 -6.080680765737252 1208 76-77 -1.518201973186283 1208 78-79 -4.1938146223616855 1208 80-81 0.7742062643494556 1208 82-83 0.2369264830362212 1208 84-85 -2.901769336033169 1208 86-87 -1.0093605352288186 1208 88-89 -5.37631696119945 1208 90-91 -0.38992495704840024 1208 92-93 -2.2064313324739118 1208 94-95 -4.853639178899886 1208 96-97 -3.7074848607356117 1208 98-99 -6.701527371554533 1208 100-101 -4.259513265236762 1209 1 1.8397669442916431 1209 2 1.3715531163469912 1209 3 0.7116842100694605 1209 4 0.31113861277482613 1209 5 0.6386381132563628 1209 6 1.1351069015163446 1209 7 0.8366567371592879 1209 8 0.38515900137550574 1209 9 -0.09604864567968718 1209 10-11 -2.908962384817759 1209 12-13 -0.18099175619643404 1209 14-15 -1.2694248310606397 1209 16-17 -0.4124774631329018 1209 18-19 -16.76001025247681 1209 20-21 -13.985249881714537 1209 22-23 -3.3869596983882175 1209 24-25 -2.172630465225879 1209 26-27 -1.8343585495652661 1209 28-29 -3.3919557512445238 1209 30-31 -0.6031481998447035 1209 32-33 -0.4589121280492563 1209 34-35 -2.9885923473527782 1209 36-37 -3.6776323632899377 1209 38-39 -1.492269296606331 1209 40-41 -9.885069725224788 1209 42-43 -5.249714376933902 1209 44-45 -2.8874767273891138 1209 46-47 -2.8923446871243073 1209 48-49 -3.6018645567369347 1209 50-51 -2.710351511538022 1209 52-53 -0.6750709114675928 1209 54-55 -2.269959814323226 1209 56-57 -0.35972296082686483 1209 58-59 -2.029178420697857 1209 60-61 -2.088273020729144 1209 62-63 -1.0220353500668438 1209 64-65 0.19996296033125205 1209 66-67 -0.03936020797976525 1209 68-69 -0.9505684684077842 1209 70-71 -1.0208030274516204 1209 72-73 -0.4812180431825688 1209 74-75 0.660889582579788 1209 76-77 -16.050241833943765 1209 78-79 -16.207953926187407 1209 80-81 -21.194308986614686 1209 82-83 -9.741710097437256 1209 84-85 -3.264388045645081 1209 86-87 -2.3166735454382774 1209 88-89 -1.9064271022620076 1209 90-91 -5.6337861590017795 1209 92-93 -3.0157751224069926 1209 94-95 -0.45015485900993113 1209 96-97 0.6141638258671804 1209 98-99 1.4890038306095867 1209 100-101 0.5262078627272615 1212 1 -28.629104651039096 1212 2 -16.25230774417185 1212 3 -5.577814033093151 1212 4 -1.1692582437866719 1212 5 -0.45562934419961465 1212 6 -2.5617277313836837 1212 7 -0.36625925592555575 1212 8 -1.5124089645178813 1212 9 0.14726185811701953 1212 10-11 0.8582770601338225 1212 12-13 0.2634167072210616 1212 14-15 -0.30210736111046543 1212 16-17 0.18144449146980435 1212 18-19 -0.600751285976564 1212 20-21 -0.6651612493958652 1212 22-23 -0.6047170151442103 1212 24-25 0.42757046189469605 1212 26-27 -0.3511492232830804 1212 28-29 0.3243685573672934 1212 30-31 -0.38359069848054617 1212 32-33 0.5967559036299193 1212 34-35 -0.6742013248173251 1212 36-37 -0.6836754759264139 1212 38-39 0.04853573779096365 1212 40-41 0.12064955816914136 1212 42-43 0.26944764478050587 1212 44-45 1.2990552909085125 1212 46-47 -1.784861426801296 1212 48-49 -1.0562875032260663 1212 50-51 0.2949266501318135 1212 52-53 -0.5678630334925714 1212 54-55 0.16071012973336352 1212 56-57 -0.8629599783340431 1212 58-59 0.030171574967422288 1212 60-61 0.7364524893656572 1212 62-63 -4.35537548296432 1212 64-65 -3.8305381845453574 1212 66-67 -1.0607342129060484 1212 68-69 0.9203129080959833 1212 70-71 -0.09526207962637656 1212 72-73 -0.557926051199253 1212 74-75 0.3525391228442558 1212 76-77 1.6087009528236003 1212 78-79 -0.09366897685266196 1212 80-81 1.0689641875748492 1212 82-83 1.2731987969026157 1212 84-85 -2.372942237321393 1212 86-87 -1.1774613939371896 1212 88-89 -0.27336133177789534 1212 90-91 0.8465418519727166 1212 92-93 -1.8979000032021318 1212 94-95 -1.6081529313334961 1212 96-97 0.6978520114544864 1212 98-99 -0.04319805524952969 1212 100-101 1.024797803112083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 4.0 10 5.0 11 13.0 12 34.0 13 221.0 14 712.0 15 1581.0 16 3025.0 17 5446.0 18 8573.0 19 12570.0 20 17854.0 21 24338.0 22 31951.0 23 40657.0 24 50777.0 25 63126.0 26 75404.0 27 90220.0 28 106022.0 29 123136.0 30 144102.0 31 164377.0 32 186563.0 33 209283.0 34 228723.0 35 241526.0 36 237293.0 37 188160.0 38 65341.0 39 174.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 29.647151988627122 25.587281321272926 21.30809766692095 23.457469023179 2 26.37562377565919 28.477817217091378 21.487695522946197 23.658863484303236 3 20.250990326145256 24.812931214606206 29.143655989022943 25.792422470225596 4 24.393194420331092 24.8599331470227 27.483949124875657 23.26292330777055 5 23.118376849355553 26.04994627811137 26.667424890596042 24.16425198193704 6 23.225303655759443 26.87434247768625 25.6374035310833 24.262950335471007 7 23.88689711504411 27.08854073773465 26.774308616094856 22.250253531126386 8 22.736852353983732 25.92738110316412 27.227433575706506 24.10833296714564 9 23.274114321212952 25.149426119263453 27.292528823279543 24.283930736244052 10-11 23.880154953399384 25.46824635729407 26.291113184738673 24.36048550456787 12-13 24.114565664569938 25.535403993712404 25.89924564361128 24.450784698106386 14-15 24.0752304775067 25.759629607530716 25.70974190870422 24.455398006258363 16-17 24.06494807875899 25.7755229173775 25.8065197087034 24.353009295160106 18-19 24.486992539994507 25.816397693294018 25.410576327151666 24.28603343955981 20-21 24.283866114886436 25.631436825729985 25.613364386049714 24.47133267333387 22-23 24.077831686350333 25.549238458840932 25.852398787875654 24.520531066933078 24-25 24.193546997390158 25.47837036998743 25.896168987435026 24.43191364518739 26-27 24.254075669024918 25.566556982682336 25.71214890139384 24.467218446898908 28-29 24.40740061019794 25.581613759007137 25.64761370558682 24.363371925208103 30-31 24.50170471141394 25.521580517780784 25.505662123354416 24.47105264745086 32-33 24.31856778392686 25.52050349515383 25.571145099073156 24.589783621846152 34-35 24.436803327913754 25.49627048604739 25.642659401502833 24.424266784536023 36-37 24.45543581936004 25.588829810607727 25.611081098080575 24.34465327195166 38-39 24.4379880528034 25.47315758047298 25.65192179609463 24.43693257062899 40-41 24.528067376264634 25.41789745574814 25.551601377492894 24.502433790494337 42-43 24.306311266432136 25.4753762470845 25.732159981802628 24.486152504680742 44-45 24.32612848276807 25.320457312423585 25.75768541806141 24.595728786746935 46-47 24.34534218017321 25.526742615735575 25.737241444095215 24.390673759996 48-49 24.50845743152395 25.474460982253678 25.661561433612512 24.355520152609863 50-51 24.519339818030133 25.403922137972522 25.542987629073387 24.53375041492395 52-53 24.266353094848725 25.577887758636436 25.76464364306233 24.391115503452507 54-55 24.31027615664465 25.379696268834028 25.912338397386314 24.397689177135014 56-57 24.38810349024313 25.466927453003528 25.625079431411113 24.519889625342227 58-59 24.345803228771526 25.446827558295702 25.828468844379405 24.37890036855336 60-61 24.539845319786178 25.4352319686228 25.552435489596654 24.472487221994367 62-63 24.475257702201503 25.361679175352954 25.603624352540315 24.559438769905224 64-65 24.40269038126659 25.408266046516438 25.72009316039666 24.468950411820305 66-67 24.518770839943844 25.484621928225398 25.603831953930733 24.392775277900025 68-69 24.55384396655903 25.437087077060276 25.532515762966344 24.476553193414343 70-71 24.621257406669024 25.500051806562695 25.47107890559552 24.407611881172762 72-73 24.41197100015838 25.50283738604935 25.647858142549158 24.437333471243118 74-75 24.470750390372633 25.335000704674858 25.629498767142238 24.564750137810268 76-77 24.398496508241138 25.59903334014333 25.622289440069483 24.38018071154605 78-79 24.663935616254822 25.549552452667946 25.374858855759097 24.411653075318142 80-81 24.57531046139587 25.41592349366893 25.416591998940742 24.592174045994454 82-83 24.482931694465808 25.53802805979023 25.439174706983497 24.539865538760466 84-85 24.49645179544988 25.435638695129274 25.517410145648395 24.550499363772445 86-87 24.53555863076825 25.550177762995315 25.365392138446612 24.54887146778983 88-89 24.525034025387527 25.635215963733827 25.349592276980225 24.49015773389842 90-91 24.6925150712698 25.531800750929612 25.21786847937723 24.557815698423358 92-93 24.479363130953978 25.46379664275363 25.31603575128518 24.74080447500721 94-95 24.578196763020873 25.533020939950628 25.233848011879882 24.65493428514862 96-97 24.61764062139162 25.617625972432386 25.159479772372407 24.605253633803596 98-99 24.60809354052328 25.542662913050705 25.158253344949205 24.6909902014768 100-101 24.678319159441845 25.475325881735543 25.018246964368657 24.82810799445396 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 2.0 3 2.0 4 3.0 5 8.5 6 14.0 7 22.5 8 32.5 9 56.0 10 87.5 11 134.0 12 206.5 13 313.0 14 482.0 15 720.0 16 1053.5 17 1560.0 18 2290.0 19 3190.0 20 4403.0 21 6091.5 22 8122.0 23 10379.5 24 13137.0 25 16196.0 26 19424.0 27 23133.0 28 27065.5 29 30840.0 30 34447.0 31 38057.5 32 41563.5 33 44739.0 34 47763.5 35 50442.0 36 52892.0 37 54855.5 38 56187.5 39 56299.0 40 55268.0 41 53966.0 42 51925.5 43 49383.5 44 47027.5 45 45229.5 46 43529.0 47 42326.5 48 41637.0 49 40775.0 50 40399.0 51 40903.0 52 42609.0 53 45701.0 54 49957.0 55 55305.0 56 61228.5 57 67484.5 58 73063.5 59 77463.5 60 79906.0 61 79041.5 62 76093.5 63 70929.0 64 63535.5 65 55546.0 66 47052.5 67 38808.0 68 31322.0 69 24633.0 70 19230.5 71 15033.5 72 11746.5 73 9169.0 74 7078.5 75 5373.5 76 4148.0 77 3165.5 78 2339.5 79 1698.0 80 1188.5 81 833.0 82 595.5 83 419.0 84 292.5 85 202.0 86 138.0 87 92.0 88 56.0 89 46.0 90 39.5 91 24.5 92 17.0 93 11.0 94 5.0 95 3.5 96 1.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6624550773862298 2 0.0028864206402339464 3 3.8772814570306745E-4 4 4.3080905078118606E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.1840416264937227 14-15 2.1540452539059303E-5 16-17 2.5848543046871165E-4 18-19 0.11127797781678035 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0017447766556638034 42-43 0.0 44-45 0.0 46-47 0.02309136512187157 48-49 4.3080905078118606E-5 50-51 2.3694497792965232E-4 52-53 8.616181015623721E-5 54-55 0.002132504801366871 56-57 0.0012493462472654396 58-59 0.09833216584080572 60-61 0.001637074392968507 62-63 0.0011631844371092024 64-65 0.002412530684374642 66-67 0.0043296309603509196 68-69 0.004695818653514928 70-71 0.003424931953710429 72-73 0.036898795199408585 74-75 0.04286550055272802 76-77 0.15261410623923516 78-79 3.0156633554683025E-4 80-81 0.11237654089627239 82-83 0.004265009602733742 84-85 0.004760440011132105 86-87 0.006289812141405316 88-89 0.006591378476952146 90-91 0.021131183940817175 92-93 0.010102472240818814 94-95 0.01359202555214642 96-97 0.00990860816796728 98-99 0.010856388079685888 100-101 0.01206265342187321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2321214.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.89190818929599 #Duplication Level Percentage of deduplicated Percentage of total 1 97.62574303747071 94.59144531298415 2 1.9057542865695696 3.6930433873131205 3 0.29768114515082683 0.8652868255691522 4 0.07778984705808323 0.3014882687684471 5 0.05194926447575335 0.25167316820430746 6 0.015177963864364898 0.08823731287478974 7 0.016755526400788246 0.11364324479819499 8 0.006527247225058383 0.05059499510873552 9 0.0014529931548574885 0.012670495142411456 >10 0.001095368643094312 0.012735882960015335 >50 0.0 0.0 >100 7.331998686547791E-5 0.01918110627668774 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.5848543046871165E-4 0.0 0.0 2 3.4464724062494885E-4 0.0 0.0 3 3.8772814570306745E-4 4.3080905078118606E-5 4.3080905078118606E-5 4 4.3080905078118605E-4 4.3080905078118606E-5 4.3080905078118606E-5 5 4.3080905078118605E-4 4.3080905078118606E-5 4.3080905078118606E-5 6 5.169708609374233E-4 4.3080905078118606E-5 4.3080905078118606E-5 7 5.169708609374233E-4 4.3080905078118606E-5 8.616181015623721E-5 8 7.323753863280163E-4 4.3080905078118606E-5 8.616181015623721E-5 9 9.046990066404907E-4 8.616181015623721E-5 8.616181015623721E-5 10-11 9.046990066404907E-4 8.616181015623721E-5 8.616181015623721E-5 12-13 0.0010124012693357872 8.616181015623721E-5 8.616181015623721E-5 14-15 0.001055482174413906 8.616181015623721E-5 1.2924271523435583E-4 16-17 0.0011201035320310838 8.616181015623721E-5 1.2924271523435583E-4 18-19 0.0012493462472654396 8.616181015623721E-5 1.2924271523435583E-4 20-21 0.0013355080574216768 8.616181015623721E-5 1.2924271523435583E-4 22-23 0.0015078316777341512 8.616181015623721E-5 1.2924271523435583E-4 24-25 0.0017232362031247442 8.616181015623721E-5 1.2924271523435583E-4 26-27 0.0018955598234372186 8.616181015623721E-5 1.7232362031247442E-4 28-29 0.0021755857064449896 8.616181015623721E-5 1.7232362031247442E-4 30-31 0.0031449060707026584 8.616181015623721E-5 1.7232362031247442E-4 32-33 0.004566575938280572 8.616181015623721E-5 1.7232362031247442E-4 34-35 0.006526757119334968 8.616181015623721E-5 1.7232362031247442E-4 36-37 0.009563960927342331 8.616181015623721E-5 1.7232362031247442E-4 38-39 0.015056776324802453 8.616181015623721E-5 1.7232362031247442E-4 40-41 0.0216481548017546 8.616181015623721E-5 1.7232362031247442E-4 42-43 0.03054436170038609 8.616181015623721E-5 1.7232362031247442E-4 44-45 0.04219774652401717 8.616181015623721E-5 1.7232362031247442E-4 46-47 0.05925778493495214 8.616181015623721E-5 1.7232362031247442E-4 48-49 0.07903192036580858 8.616181015623721E-5 1.7232362031247442E-4 50-51 0.1069698873089685 8.616181015623721E-5 1.7232362031247442E-4 52-53 0.13895745932947157 1.5078316777341512E-4 1.7232362031247442E-4 54-55 0.1744130442087632 1.7232362031247442E-4 1.9386407285153372E-4 56-57 0.21820478422067074 1.7232362031247442E-4 2.1540452539059302E-4 58-59 0.27280983140718607 1.7232362031247442E-4 2.1540452539059302E-4 60-61 0.337086541783739 1.7232362031247442E-4 2.1540452539059302E-4 62-63 0.4097855691030642 1.7232362031247442E-4 2.1540452539059302E-4 64-65 0.49189777418195824 1.7232362031247442E-4 2.1540452539059302E-4 66-67 0.5853402572963975 1.7232362031247442E-4 2.5848543046871165E-4 68-69 0.6937748953780221 1.7232362031247442E-4 2.5848543046871165E-4 70-71 0.817998685170777 1.7232362031247442E-4 2.5848543046871165E-4 72-73 0.9486415298201716 1.7232362031247442E-4 2.5848543046871165E-4 74-75 1.09363031586058 1.7232362031247442E-4 3.4464724062494885E-4 76-77 1.2575316192302821 1.7232362031247442E-4 3.6618769316400815E-4 78-79 1.442757970613653 1.7232362031247442E-4 3.8772814570306745E-4 80-81 1.6580332532890116 1.7232362031247442E-4 3.8772814570306745E-4 82-83 1.8865774547284309 1.7232362031247442E-4 3.8772814570306745E-4 84-85 2.1211960637838647 1.7232362031247442E-4 3.8772814570306745E-4 86-87 2.3672095722324613 1.7232362031247442E-4 3.8772814570306745E-4 88-89 2.641721099390233 1.7232362031247442E-4 3.8772814570306745E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAG 2425 0.0 6.0670977 94-95 AGTCACG 2210 0.0 6.013272 92-93 ACGTCTG 5015 0.0 5.5362535 94-95 CACGTCT 5205 0.0 5.425344 94-95 CACGAGT 1845 0.0 5.401993 94-95 TGGATCT 1715 0.0 5.396368 94-95 TCTGAAC 4535 0.0 5.337329 94-95 CAGTCAC 3040 0.0 5.308067 94-95 CTCGTAT 805 6.525251E-5 5.306299 92-93 CGTATGC 810 7.199512E-5 5.273373 94-95 TCGTATG 810 7.199512E-5 5.273373 94-95 CGTCTGA 4550 0.0 5.215593 92-93 CACACGT 5840 0.0 5.160502 94-95 >>END_MODULE