FastQCFastQC Report
Thu 11 Sep 2014
NYCLib23_GAGTGG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib23_GAGTGG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321214
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG24250.06.067097794-95
AGTCACG22100.06.01327292-93
ACGTCTG50150.05.536253594-95
CACGTCT52050.05.42534494-95
CACGAGT18450.05.40199394-95
TGGATCT17150.05.39636894-95
TCTGAAC45350.05.33732994-95
CAGTCAC30400.05.30806794-95
CTCGTAT8056.525251E-55.30629992-93
CGTATGC8107.199512E-55.27337394-95
TCGTATG8107.199512E-55.27337394-95
CGTCTGA45500.05.21559392-93
CACACGT58400.05.16050294-95
Produced by FastQC (version 0.11.2)