##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib22_CGTACG_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8876048 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.972141881161527 33.0 22.0 33.0 2.0 33.0 2 25.024971811779295 33.0 15.0 33.0 2.0 33.0 3 25.895953807370127 33.0 22.0 33.0 6.0 33.0 4 28.026879192181024 33.0 22.0 37.0 2.0 37.0 5 28.07549936638468 33.0 22.0 37.0 6.0 37.0 6 29.13526053486867 33.0 27.0 37.0 15.0 37.0 7 28.81389555351661 33.0 27.0 37.0 6.0 37.0 8 28.580571556170042 33.0 27.0 37.0 2.0 37.0 9 29.35846437513632 33.0 27.0 37.0 15.0 37.0 10-11 28.994065320512014 33.0 27.0 37.0 10.5 37.0 12-13 29.300981810823913 33.0 27.0 37.0 10.5 37.0 14-15 29.678078971632424 33.0 27.0 37.0 6.0 40.0 16-17 30.57840116457234 33.0 27.0 37.0 15.0 40.0 18-19 30.917937577624635 33.0 27.0 37.0 15.0 40.0 20-21 30.951063750443893 33.0 27.0 37.0 15.0 40.0 22-23 31.001479768924188 33.0 27.0 37.0 15.0 40.0 24-25 30.615163358738034 33.0 27.0 37.0 10.5 40.0 26-27 30.127700695174248 33.0 27.0 37.0 15.0 40.0 28-29 30.35383534428836 33.0 27.0 37.0 15.0 40.0 30-31 30.28992593325318 33.0 27.0 37.0 15.0 40.0 32-33 30.1516010841762 33.0 27.0 37.0 15.0 40.0 34-35 30.21823439891267 33.0 27.0 37.0 15.0 40.0 36-37 30.458630406234846 33.0 27.0 37.0 15.0 40.0 38-39 30.52217788817726 33.0 27.0 37.0 15.0 40.0 40-41 30.631681971526067 33.0 27.0 37.0 15.0 40.0 42-43 30.580294405798618 33.0 27.0 37.0 15.0 40.0 44-45 30.359096413178477 33.0 27.0 37.0 15.0 40.0 46-47 30.007613974147052 33.0 27.0 37.0 15.0 40.0 48-49 29.875131815420556 33.0 27.0 37.0 15.0 40.0 50-51 29.13837571630978 33.0 24.5 37.0 15.0 38.5 52-53 28.860483010006256 33.0 22.0 37.0 10.5 38.5 54-55 29.830939681714206 33.0 27.0 37.0 15.0 40.0 56-57 29.769403511562803 33.0 27.0 37.0 15.0 40.0 58-59 29.98512406647643 33.0 27.0 37.0 15.0 40.0 60-61 29.75806147060043 33.0 27.0 37.0 15.0 40.0 62-63 29.65570251535368 33.0 27.0 37.0 15.0 40.0 64-65 29.631352489306053 33.0 27.0 37.0 15.0 40.0 66-67 29.523198387390423 33.0 27.0 37.0 15.0 40.0 68-69 29.250195920526792 33.0 27.0 37.0 15.0 40.0 70-71 29.160045382810008 33.0 27.0 37.0 15.0 40.0 72-73 28.932524869176014 33.0 27.0 37.0 15.0 37.0 74-75 28.89933166201895 33.0 27.0 37.0 15.0 37.0 76-77 28.50039538993029 33.0 27.0 37.0 15.0 37.0 78-79 27.928660198773148 33.0 22.0 37.0 10.5 37.0 80-81 27.80271980277709 33.0 22.0 37.0 15.0 37.0 82-83 27.370031854266674 30.0 22.0 33.0 10.5 37.0 84-85 27.474940592930547 30.0 22.0 33.0 15.0 37.0 86-87 27.164812932512305 27.0 22.0 33.0 10.5 37.0 88-89 26.80235978895112 27.0 22.0 33.0 6.0 37.0 90-91 26.673366288690644 27.0 22.0 33.0 6.0 37.0 92-93 26.55869825174447 27.0 22.0 33.0 6.0 37.0 94-95 26.345968723918574 27.0 22.0 33.0 6.0 37.0 96-97 26.220026074667466 27.0 22.0 33.0 6.0 37.0 98-99 25.988827291154802 27.0 22.0 33.0 6.0 37.0 100-101 25.50328175332085 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.342035234129295 2306 2 8.47052031591181 2306 3 7.513501039791564 2306 4 9.094807225550635 2306 5 8.957273484505695 2306 6 7.908529954593423 2306 7 8.16805416201105 2306 8 8.605713295649444 2306 9 7.520506219686219 2306 10-11 7.8241220467201416 2306 12-13 7.479444195167776 2306 14-15 8.102308472130257 2306 16-17 6.834447846749985 2306 18-19 6.216437517263088 2306 20-21 6.141254036739255 2306 22-23 5.805858324254544 2306 24-25 5.878117909364349 2306 26-27 6.625558919880401 2306 28-29 5.615919881293127 2306 30-31 5.597084186659043 2306 32-33 5.521416141391761 2306 34-35 4.8664624334065465 2306 36-37 4.955107353452995 2306 38-39 4.670648711076328 2306 40-41 3.8952423206904925 2306 42-43 3.685436325003014 2306 44-45 3.384348494491597 2306 46-47 3.721128861879457 2306 48-49 3.5456392515178905 2306 50-51 3.4030124087442424 2306 52-53 3.967694377181786 2306 54-55 3.5392760566948773 2306 56-57 3.7868956348577036 2306 58-59 3.312105144036508 2306 60-61 3.391621242808867 2306 62-63 2.910732247690241 2306 64-65 2.5322444364799743 2306 66-67 2.3846349718109003 2306 68-69 2.519396354404279 2306 70-71 2.6331462779917807 2306 72-73 1.8814079926490805 2306 74-75 1.4760684080624635 2306 76-77 1.8316844010642903 2306 78-79 2.0987226900781515 2306 80-81 1.8910935916487155 2306 82-83 1.8657998900243236 2306 84-85 1.3887941708334957 2306 86-87 1.3179260394379853 2306 88-89 1.3318757051716936 2306 90-91 1.226181507165581 2306 92-93 1.2443075454114485 2306 94-95 1.5455101880029858 2306 96-97 1.1909883097620657 2306 98-99 1.0225722525989376 2306 100-101 0.8337559108427364 2307 1 8.294903210752675 2307 2 8.414198216011123 2307 3 7.576153044736724 2307 4 9.139136654114967 2307 5 9.010166995199949 2307 6 7.605613244390472 2307 7 8.122801969876644 2307 8 8.516654091412398 2307 9 7.570498222430807 2307 10-11 7.788185421944199 2307 12-13 7.358127662391549 2307 14-15 8.180648476499368 2307 16-17 6.845726027875596 2307 18-19 6.471536201868808 2307 20-21 6.429041773421588 2307 22-23 6.031009313984441 2307 24-25 6.1788632873371085 2307 26-27 6.9545056493059825 2307 28-29 6.116176888773179 2307 30-31 5.947221919333561 2307 32-33 6.07810280558035 2307 34-35 5.739126665796981 2307 36-37 5.663358283073059 2307 38-39 5.249498033140906 2307 40-41 4.656704467642626 2307 42-43 4.456792787775715 2307 44-45 4.420661572830127 2307 46-47 4.807440255588396 2307 48-49 4.608517162152488 2307 50-51 4.398896767467718 2307 52-53 4.950113791385693 2307 54-55 4.558254683469695 2307 56-57 4.917748344083019 2307 58-59 4.518917816407992 2307 60-61 4.478694756883847 2307 62-63 4.193960300062095 2307 64-65 4.00820018182883 2307 66-67 3.706460165044355 2307 68-69 3.9123696001377404 2307 70-71 3.550091676987506 2307 72-73 2.9771615255170225 2307 74-75 2.6159836113959045 2307 76-77 2.8108951086457346 2307 78-79 3.2592216426206058 2307 80-81 2.997234206668722 2307 82-83 3.1285413786727787 2307 84-85 2.34091267940045 2307 86-87 2.342119697409885 2307 88-89 2.5236214046689724 2307 90-91 2.292008331323956 2307 92-93 2.178918377251776 2307 94-95 2.3310954162550885 2307 96-97 2.14408261282642 2307 98-99 1.9830786566374279 2307 100-101 1.6479275309589703 1101 1 8.0328724309628 1101 2 7.8700309592205855 1101 3 7.299489548192536 1101 4 8.755653145255646 1101 5 8.5545573059016 1101 6 7.414327604543164 1101 7 7.06693623663865 1101 8 8.059906393182423 1101 9 7.365005565599411 1101 10-11 7.577271360717454 1101 12-13 7.226660587429944 1101 14-15 7.792558773462698 1101 16-17 6.929420327881569 1101 18-19 6.048001711615321 1101 20-21 5.858645094690388 1101 22-23 5.3717863557652095 1101 24-25 6.0034478131128 1101 26-27 6.601120663635381 1101 28-29 5.990547018816589 1101 30-31 5.761981617928509 1101 32-33 5.887944701639739 1101 34-35 5.679941378187635 1101 36-37 5.517922344844969 1101 38-39 5.143831344763033 1101 40-41 4.650642307800737 1101 42-43 4.546029703093982 1101 44-45 4.45468012773059 1101 46-47 4.733459291674137 1101 48-49 4.484858982605253 1101 50-51 4.075834739758108 1101 52-53 4.937357126955188 1101 54-55 4.6766318760620145 1101 56-57 4.821775283142063 1101 58-59 4.392637632576275 1101 60-61 4.565656102670424 1101 62-63 4.145293587868856 1101 64-65 3.9395911803069446 1101 66-67 3.5605441713293153 1101 68-69 3.7622860956971778 1101 70-71 3.7168787953963864 1101 72-73 3.231810992655255 1101 74-75 2.6778718540337962 1101 76-77 3.0219068702831287 1101 78-79 3.5243888781781614 1101 80-81 3.08848805801604 1101 82-83 3.1965493469231596 1101 84-85 2.536414662245349 1101 86-87 2.628578610585354 1101 88-89 2.6540385050814663 1101 90-91 2.528474224124942 1101 92-93 2.2890182396964818 1101 94-95 2.573340930357233 1101 96-97 2.429969042483272 1101 98-99 2.2712899184963327 1101 100-101 1.9860449982716553 2201 1 7.089158832532149 2201 2 7.84221487253544 2201 3 6.302659712307211 2201 4 8.422297411775535 2201 5 4.040922233027537 2201 6 5.814875358344626 2201 7 6.64760069518648 2201 8 7.687871858356836 2201 9 6.527668243200651 2201 10-11 4.1516838213697085 2201 12-13 6.373679358540013 2201 14-15 7.057822596565199 2201 16-17 5.9116324255666335 2201 18-19 2.141616161173719 2201 20-21 5.083582159602912 2201 22-23 5.166490003144965 2201 24-25 5.497041673553255 2201 26-27 6.203832975119507 2201 28-29 5.062826553847916 2201 30-31 5.290971267265451 2201 32-33 5.39974402897672 2201 34-35 4.908171359670426 2201 36-37 4.97859324308164 2201 38-39 4.503143037685913 2201 40-41 4.277228850557641 2201 42-43 4.1543629035279785 2201 44-45 3.9330744028081597 2201 46-47 4.411752284790012 2201 48-49 4.138115254752812 2201 50-51 3.8822442544835845 2201 52-53 4.6904585886283705 2201 54-55 4.301565469988926 2201 56-57 4.423875078279568 2201 58-59 4.026515577302547 2201 60-61 4.011143038558991 2201 62-63 3.793888459317163 2201 64-65 3.6300884036506105 2201 66-67 3.2805416555668643 2201 68-69 3.5104727879372035 2201 70-71 3.423204943963867 2201 72-73 2.725337564545306 2201 74-75 2.3656633923204353 2201 76-77 2.7095562471203323 2201 78-79 3.0601606368249747 2201 80-81 2.571418596858827 2201 82-83 2.824099280930927 2201 84-85 2.3830883866562083 2201 86-87 2.3337901820551217 2201 88-89 2.4443135440437302 2201 90-91 2.251482279003323 2201 92-93 2.141734795607338 2201 94-95 2.2671542143054637 2201 96-97 2.218788789634715 2201 98-99 2.0416744614104267 2201 100-101 1.6694702894115174 2304 1 1.812886946194869 2304 2 5.000518488066376 2304 3 5.859833922683304 2304 4 4.296466941292184 2304 5 6.606762476410939 2304 6 6.376985126476836 2304 7 7.11071847291495 2304 8 6.554183748799289 2304 9 5.913209775626733 2304 10-11 6.746934408549066 2304 12-13 6.622082522874262 2304 14-15 6.827020290739792 2304 16-17 5.52507871190323 2304 18-19 5.301705675567476 2304 20-21 5.034999668871237 2304 22-23 4.651128089588994 2304 24-25 4.889895540240083 2304 26-27 5.7760087135771165 2304 28-29 5.0283926536297905 2304 30-31 5.107700007813609 2304 32-33 5.217934474179721 2304 34-35 4.795007734603736 2304 36-37 4.939653304777401 2304 38-39 4.507610104459911 2304 40-41 3.895731382878658 2304 42-43 3.4318436868513835 2304 44-45 3.294978502081719 2304 46-47 4.280484522093879 2304 48-49 3.990603402401355 2304 50-51 3.5715903469694013 2304 52-53 4.211855977740218 2304 54-55 3.968313761544298 2304 56-57 4.622819906458648 2304 58-59 3.8427716803894754 2304 60-61 3.929416029624548 2304 62-63 3.6304893294389444 2304 64-65 3.4880733322946327 2304 66-67 3.3633644777585445 2304 68-69 3.5069016157655106 2304 70-71 3.4847556555798853 2304 72-73 3.0995908012182305 2304 74-75 2.5011751017240798 2304 76-77 2.703060618971854 2304 78-79 3.1182229713050127 2304 80-81 2.832955448086473 2304 82-83 3.053726128948785 2304 84-85 2.5406864402370566 2304 86-87 2.4269453277554938 2304 88-89 2.664548048982418 2304 90-91 2.566176742570942 2304 92-93 2.2840063038933636 2304 94-95 2.5491913613554793 2304 96-97 2.5195818595929005 2304 98-99 2.420524495660203 2304 100-101 2.053848908308826 1102 1 6.955182482874083 1102 2 7.433752438794624 1102 3 6.008843773421827 1102 4 6.1076284155841485 1102 5 6.4451823059016 1102 6 5.6823067579584325 1102 7 6.7023604907987355 1102 8 6.299104794807469 1102 9 4.735454351942437 1102 10-11 5.93291908472591 1102 12-13 5.8632356292359695 1102 14-15 6.079795626367069 1102 16-17 5.200229562908344 1102 18-19 5.283081226409003 1102 20-21 5.450970195486491 1102 22-23 5.09295382734738 1102 24-25 1.812951020378332 1102 26-27 5.452023692904721 1102 28-29 4.806510923293683 1102 30-31 4.777875279315136 1102 32-33 4.924211103449451 1102 34-35 4.884614547443029 1102 36-37 4.810086312928767 1102 38-39 3.8656427380576552 1102 40-41 3.9136509049651096 1102 42-43 4.058574073900562 1102 44-45 -4.0288775068694065 1102 46-47 1.4019681696826325 1102 48-49 -0.7860089219128774 1102 50-51 2.6882533398463337 1102 52-53 -0.1251199502654039 1102 54-55 2.6233376963320403 1102 56-57 -0.8130201174262943 1102 58-59 2.8529241577009223 1102 60-61 3.8056625723443886 1102 62-63 3.3995831933020995 1102 64-65 3.0532862425799614 1102 66-67 3.072365913537496 1102 68-69 3.3631883234707374 1102 70-71 3.1708437629774835 1102 72-73 2.6580831588226417 1102 74-75 2.4262129180611254 1102 76-77 2.7900306135377413 1102 78-79 -1.395211476788294 1102 80-81 1.612200951967246 1102 82-83 2.5331552944065905 1102 84-85 2.220650490517503 1102 86-87 2.290980411321957 1102 88-89 2.306576297210647 1102 90-91 2.135760365814008 1102 92-93 2.084830135689259 1102 94-95 2.3842263972509663 1102 96-97 2.36054251592234 1102 98-99 2.2926616432639655 1102 100-101 1.900522993685815 2202 1 -20.726742953652582 2202 2 -7.79264176359646 2202 3 -0.6891134036302979 2202 4 1.7341176302951204 2202 5 1.0934479853970736 2202 6 1.6298918443278616 2202 7 3.9799158395844962 2202 8 2.795429004781095 2202 9 3.248948590335406 2202 10-11 2.0903628414033584 2202 12-13 1.4527318734530503 2202 14-15 2.768448277120676 2202 16-17 1.7507650951779112 2202 18-19 1.7231359504093113 2202 20-21 1.1999155733168934 2202 22-23 1.4039498110704685 2202 24-25 2.4227132130453484 2202 26-27 3.6205952431149413 2202 28-29 -0.8708497751510151 2202 30-31 1.0465517337164947 2202 32-33 1.6095690833059635 2202 34-35 1.6368276846177992 2202 36-37 2.9559863953054943 2202 38-39 2.450038471588506 2202 40-41 1.8923880295101654 2202 42-43 0.7580760881003634 2202 44-45 0.8281006095833447 2202 46-47 -1.315466456649716 2202 48-49 0.8728761436054988 2202 50-51 1.8059630451432582 2202 52-53 1.7584916024907464 2202 54-55 -0.7931182620747812 2202 56-57 1.1701542533621883 2202 58-59 1.9423033203446352 2202 60-61 2.1309039972445163 2202 62-63 -2.0817580552576302 2202 64-65 0.08397005195233831 2202 66-67 0.5628741953272964 2202 68-69 1.4447937541262839 2202 70-71 -2.5536153953481673 2202 72-73 -4.726463757953532 2202 74-75 -1.1741915101435296 2202 76-77 -0.1447995349326483 2202 78-79 1.5383400189955694 2202 80-81 1.5242356398184995 2202 82-83 -0.8051027767788028 2202 84-85 -0.2622375873823408 2202 86-87 0.6301533036323796 2202 88-89 1.1508717711562824 2202 90-91 1.294705127166818 2202 92-93 1.2325936491713847 2202 94-95 1.6176293424506198 2202 96-97 1.1962149671698334 2202 98-99 1.1709158556997679 2202 100-101 0.9144083142895987 2305 1 5.771567400497187 2305 2 7.105474886260431 2305 3 6.729418451398772 2305 4 7.744229528776099 2305 5 7.901498462035274 2305 6 6.197731733472526 2305 7 3.0486060514715483 2305 8 6.1398051116284265 2305 9 6.42748816055186 2305 10-11 4.8987492148288005 2305 12-13 5.271290300976769 2305 14-15 5.6384683273419505 2305 16-17 4.985194682685499 2305 18-19 4.40643844251565 2305 20-21 5.204253698061905 2305 22-23 5.112585845605189 2305 24-25 5.227636304183335 2305 26-27 6.100916401557409 2305 28-29 5.147391312187484 2305 30-31 5.346617320676518 2305 32-33 5.3403540038239115 2305 34-35 5.2727250589890104 2305 36-37 4.689760069505702 2305 38-39 4.583513799277089 2305 40-41 4.118605209733012 2305 42-43 4.06296306255749 2305 44-45 3.273344996987035 2305 46-47 4.001924683827944 2305 48-49 3.4615937106366914 2305 50-51 2.406139375841164 2305 52-53 3.9269759696935758 2305 54-55 4.2398933822112035 2305 56-57 4.686214127738491 2305 58-59 4.401576692644937 2305 60-61 4.525730117352602 2305 62-63 3.8401619130331213 2305 64-65 4.089221102657827 2305 66-67 3.6825910489994094 2305 68-69 4.055243095170958 2305 70-71 3.7678226490863906 2305 72-73 -0.8991216633165777 2305 74-75 2.204252897936559 2305 76-77 3.111512448736491 2305 78-79 3.7231721523343317 2305 80-81 3.336077974582569 2305 82-83 3.473280257711213 2305 84-85 2.9451795856608776 2305 86-87 2.7942145720921694 2305 88-89 2.9084750827834753 2305 90-91 2.840126173072097 2305 92-93 2.8644509670403124 2305 94-95 3.093333961557022 2305 96-97 2.9855615413824594 2305 98-99 2.905757557992139 2305 100-101 2.4955085761187803 1103 1 -20.726742953652582 1103 2 -7.862696838323407 1103 3 -0.7045148111327535 1103 4 3.066975690961087 1103 5 4.720423897094005 1103 6 4.6123490460612615 1103 7 4.039687658211204 1103 8 4.484022051668308 1103 9 4.538922536073677 1103 10-11 5.024981422928267 1103 12-13 4.702607823082559 1103 14-15 4.237815999495076 1103 16-17 2.989521416702665 1103 18-19 3.2738630329889205 1103 20-21 2.5129776751896067 1103 22-23 2.827488293261105 1103 24-25 3.158799201764541 1103 26-27 4.257164275264227 1103 28-29 3.779183169956415 1103 30-31 3.638776982046771 1103 32-33 3.0566172064204835 1103 34-35 2.6018344618543523 1103 36-37 2.902305673224088 1103 38-39 2.566633745338688 1103 40-41 2.6957580060961703 1103 42-43 2.360546140599954 1103 44-45 2.714186437034222 1103 46-47 2.086577414185143 1103 48-49 -0.9751349816618458 1103 50-51 -1.7594735265804786 1103 52-53 1.828174441092564 1103 54-55 2.4982674589565264 1103 56-57 3.4322685514615294 1103 58-59 2.89989664722043 1103 60-61 3.1506285883677663 1103 62-63 2.9565021157933344 1103 64-65 2.834758208337181 1103 66-67 2.6369626292657316 1103 68-69 2.956070106180519 1103 70-71 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48-49 -20.193132588217622 1209 50-51 -22.534975962276388 1209 52-53 -25.076099185753844 1209 54-55 -22.236049415386248 1209 56-57 -25.9696388054867 1209 58-59 -26.278505762072555 1209 60-61 -26.038780561209173 1209 62-63 -26.199815107783316 1209 64-65 -26.088732398288375 1209 66-67 -13.85515501135788 1209 68-69 -25.78761984075767 1209 70-71 -25.638942692897928 1209 72-73 -15.958997573081392 1209 74-75 -16.990805867037256 1209 76-77 -13.956251554089885 1209 78-79 -14.717572917865702 1209 80-81 -24.70156733496055 1209 82-83 -16.540535212140878 1209 84-85 -13.755350740053267 1209 86-87 -13.980221160554798 1209 88-89 -11.107842351849953 1209 90-91 -13.94075285258144 1209 92-93 -18.10766270473115 1209 94-95 -23.406801210110995 1209 96-97 -19.164059928373128 1209 98-99 -10.588275125129117 1209 100-101 -5.870136734746314 1212 1 -20.726742953652582 1212 2 -20.550851147802828 1212 3 -21.440424749466327 1212 4 -23.436968092202545 1212 5 -23.60289461717532 1212 6 -24.668448315189277 1212 7 -24.24153993392991 1212 8 -23.890135412837644 1212 9 -24.820822193597913 1212 10-11 -24.55823240182435 1212 12-13 -24.848302832302497 1212 14-15 -25.203887714831612 1212 16-17 -26.276970767436158 1212 18-19 -20.92089342891367 1212 20-21 -26.715116401965133 1212 22-23 -26.9350959602883 1212 24-25 -22.996683160921233 1212 26-27 -25.70382812399004 1212 28-29 -20.616116789784485 1212 30-31 -15.474044917996931 1212 32-33 -25.923771435484007 1212 34-35 -22.117968948229542 1212 36-37 -26.393370506325603 1212 38-39 -26.686592986340646 1212 40-41 -21.223576454582563 1212 42-43 -17.457521563106674 1212 44-45 -14.334239799211247 1212 46-47 -17.661976248792506 1212 48-49 -20.692105925104492 1212 50-51 -12.591849545207051 1212 52-53 -5.262197132041365 1212 54-55 -4.214776862981239 1212 56-57 -4.185278615913237 1212 58-59 -2.8843509437471226 1212 60-61 -14.552682614921652 1212 62-63 -12.31008367018458 1212 64-65 -16.751291639994534 1212 66-67 -13.088920176655275 1212 68-69 -8.374823632226864 1212 70-71 -15.747315520702035 1212 72-73 -12.254175578234019 1212 74-75 -8.598528243117318 1212 76-77 -19.741715271321063 1212 78-79 -10.32954524662965 1212 80-81 -8.02905548661932 1212 82-83 -5.409118084866325 1212 84-85 -4.8732184635108595 1212 86-87 -3.712443241150556 1212 88-89 -3.593182467803402 1212 90-91 -3.966519083939854 1212 92-93 -3.8131517602242653 1212 94-95 -3.6298659810430642 1212 96-97 -13.598608030514134 1212 98-99 -10.886991603110708 1212 100-101 -6.98692371953177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1432.0 3 3243.0 4 1360.0 5 8696.0 6 11691.0 7 9513.0 8 20005.0 9 39965.0 10 51414.0 11 58281.0 12 77394.0 13 99125.0 14 114471.0 15 122230.0 16 128925.0 17 140877.0 18 161440.0 19 181492.0 20 197989.0 21 225054.0 22 255014.0 23 280722.0 24 298221.0 25 310306.0 26 331181.0 27 349355.0 28 358847.0 29 365232.0 30 378620.0 31 401891.0 32 441754.0 33 517861.0 34 602821.0 35 671320.0 36 718160.0 37 679387.0 38 259845.0 39 914.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.6275502806468 29.709752296705783 18.23538017616024 22.42731724648718 2 23.065107364510418 31.15595447248803 24.087267910629603 21.691670252371953 3 19.25446840993135 29.36684479983654 29.50787261686157 21.870814173370544 4 22.329086874374156 27.803252391854883 28.43977466088382 21.42788607288714 5 21.689893989495122 29.717500971950134 27.46502127370203 21.12758376485272 6 21.710975797604267 29.297873321349616 27.468222286208437 21.52292859483768 7 21.672543637743328 29.449138071297092 28.68140448432935 20.19691380663023 8 20.654925106072717 28.28705653904917 29.727460872577176 21.33055748230094 9 21.454146436928475 28.017913596900424 28.989322657620818 21.538617308550283 10-11 22.044280079851237 28.183324122337357 28.03329755481917 21.739098242992235 12-13 21.628279930275976 28.480750236206216 28.164770066804955 21.726199766712856 14-15 21.571730294736692 28.744324761879557 28.247608148554427 21.436336794829323 16-17 21.746460803991933 28.771372871282637 28.112149699386634 21.370016625338796 18-19 21.990211001561242 28.621473449220296 27.833268077996525 21.55504747122193 20-21 21.95155151616292 28.581587645668606 27.832889624897987 21.63397121327049 22-23 21.635294426336937 28.395578155046625 28.258200549428974 21.710926869187468 24-25 21.6324799359197 28.471693186018893 28.316626481169138 21.579200396892265 26-27 21.545891363376406 28.718011163353175 28.162459142443154 21.57363833082727 28-29 21.926974957719445 28.553311404920972 28.01654488218759 21.503168755171995 30-31 21.92720966660756 28.40215087137316 27.88437348457719 21.786265977442092 32-33 21.527376221728225 28.610330862034374 28.15669556010181 21.705597356135595 34-35 21.67629521139405 28.49490074118514 28.25611344514989 21.57269060227092 36-37 21.74027595378267 28.671741645343246 28.085334769205545 21.50264763166854 38-39 21.704206969634484 28.64704470423388 28.203989881684638 21.444758444446997 40-41 21.994774621348135 28.46785491594823 27.900284637227173 21.637085825476458 42-43 21.696695926952966 28.503132513959212 28.088249097496444 21.711922461591378 44-45 21.557579898155442 28.443441926885736 28.382406433364793 21.616571741594033 46-47 21.699847720587357 28.743214705974257 28.096731273879527 21.46020629955886 48-49 21.739807546337065 28.593762938143495 28.18579714771484 21.480632367804596 50-51 21.920021938294312 28.52069822787728 27.903901160476025 21.65537867335238 52-53 21.6184542441042 28.553683940877434 28.264679206420173 21.56318260859819 54-55 21.675038483002204 28.39299860413938 28.217978053570636 21.71398485928778 56-57 21.63194942035065 28.614411382395367 28.243072395520702 21.510566801733287 58-59 21.759052609848357 28.64688907957762 28.09971556752826 21.494342743045763 60-61 21.896951656626875 28.307638847267906 28.01361837947592 21.7817911166293 62-63 21.681500996342344 28.461758284933165 28.145387426351203 21.711353292373285 64-65 21.743404603334845 28.46356489594188 28.170176252835116 21.622854247888156 66-67 21.868320038207024 28.489069159867558 28.0431732564434 21.599437545482022 68-69 21.81350211622231 28.539101273367944 28.13363775798558 21.51375885242416 70-71 21.99954633469505 28.53846133709711 27.94317642879816 21.518815899409677 72-73 21.74850396898823 28.510346521273345 28.0706507981557 21.67049871158272 74-75 21.777290938875403 28.435859914455452 28.11492405595076 21.67192509071839 76-77 21.964301681858363 28.6359222037223 27.97440709370936 21.425369020709983 78-79 22.099622634045847 28.54731357009608 27.857208501325132 21.49585529453294 80-81 22.192900618936363 28.494287616961945 27.715429273225055 21.597382490876633 82-83 22.042019075549987 28.45953008524858 27.927352184193282 21.571098655008154 84-85 22.15974103476851 28.257150846954225 27.83664293540226 21.746465182875003 86-87 22.0568334673005 28.575171086549368 27.93266437150979 21.43533107464034 88-89 22.178516162873088 28.55688971414989 27.90805092523896 21.35654319773806 90-91 22.39183800960287 28.369067835790435 27.584729899483325 21.654364255123372 92-93 22.284269636546092 28.385743125171825 27.68704245494463 21.642944783337448 94-95 22.404700950637764 28.33973714755702 27.660131337375255 21.59543056442996 96-97 22.462677975945994 28.463734432476347 27.613661242723996 21.45992634885366 98-99 22.55036072807377 28.582132080757543 27.538977177093553 21.32853001407513 100-101 23.015353396881892 28.427266295869497 27.084503249039177 21.472877058209434 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 613.0 1 675.5 2 728.5 3 776.0 4 888.5 5 1006.0 6 1242.0 7 1511.0 8 1831.5 9 2385.5 10 3175.0 11 4259.0 12 5720.0 13 7629.0 14 9849.0 15 12564.5 16 15840.0 17 19846.0 18 24996.5 19 31734.0 20 40272.0 21 50878.0 22 63307.5 23 77396.0 24 93570.0 25 111671.5 26 130980.5 27 151078.0 28 171614.0 29 191751.0 30 210803.0 31 228850.5 32 246704.5 33 263177.5 34 278083.5 35 291816.0 36 302731.5 37 309817.5 38 310711.0 39 306196.5 40 297671.5 41 284128.5 42 268147.0 43 251104.0 44 234239.5 45 218960.5 46 206161.0 47 195624.5 48 185988.0 49 176653.5 50 167850.5 51 159743.5 52 152175.5 53 146338.5 54 142767.0 55 140098.0 56 138677.0 57 137615.0 58 134994.5 59 131134.5 60 126028.0 61 119644.0 62 111984.5 63 103237.5 64 93889.0 65 84371.5 66 74592.5 67 65301.5 68 56723.5 69 48644.0 70 41543.5 71 35272.0 72 29647.0 73 24707.0 74 20457.0 75 17121.0 76 14180.5 77 11531.0 78 9492.5 79 7850.5 80 6306.5 81 5052.0 82 4055.5 83 3262.5 84 2710.5 85 2222.5 86 1801.0 87 1414.0 88 1093.0 89 880.5 90 714.0 91 535.5 92 401.0 93 309.5 94 224.5 95 153.0 96 102.5 97 70.0 98 41.5 99 20.0 100 9.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.750691749301042 2 11.133851461821747 3 9.791711356225202 4 11.701333746730528 5 9.210258889992483 6 5.428981456612222 7 9.775420322197446 8 10.162597137825301 9 5.5307159222212405 10-11 6.42011512330713 12-13 5.441137767619102 14-15 7.41073617447765 16-17 2.715577923868821 18-19 3.1438541116496888 20-21 3.122115833533122 22-23 3.175427848069321 24-25 3.554943596519532 26-27 4.3784576198776755 28-29 1.7644620669018465 30-31 2.283966918610625 32-33 3.0880184514549716 34-35 2.0116892112345495 36-37 3.1244254199616766 38-39 1.408132312939272 40-41 1.1658116314828402 42-43 0.5560075835552039 44-45 1.3205201233702206 46-47 1.3901175387965454 48-49 1.2889351206753277 50-51 1.5639223672517317 52-53 2.4956883964575223 54-55 1.8339862515389733 56-57 1.846852337887312 58-59 1.5759209504049547 60-61 1.4058790578870237 62-63 0.6115446874554983 64-65 1.2626734330413716 66-67 0.5683328886910031 68-69 0.8075666107258547 70-71 1.0121058380937102 72-73 0.08353943106211233 74-75 0.10503548426056282 76-77 0.46392268270743914 78-79 0.8857489279012463 80-81 0.2941342813828857 82-83 0.5194428871948417 84-85 0.06559788770858382 86-87 0.09943051231809472 88-89 0.08116224698199019 90-91 0.08864305375545513 92-93 0.17093192826356957 94-95 0.45694322518309954 96-97 0.37494164069414676 98-99 0.06526553258837717 100-101 0.2293025003920664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8876048.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.34303066346757 #Duplication Level Percentage of deduplicated Percentage of total 1 98.21815243296045 95.608526239908 2 1.3167604486554705 2.5635490545982154 3 0.23134024535026373 0.675580817904745 4 0.11893677791288869 0.46310665677553425 5 0.06174636469006189 0.30052891356911726 6 0.023594310025297924 0.13780449865655617 7 0.009196730759848486 0.06266663510517106 8 0.006440612109011353 0.05015589616151942 9 0.00429567570428025 0.03763386826218586 >10 0.009536401832425346 0.10044741905898065 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.886392682869674E-5 1.126627526124239E-5 0.0 2 9.013020208993912E-5 2.253255052248478E-5 1.126627526124239E-5 3 9.013020208993912E-5 2.253255052248478E-5 1.126627526124239E-5 4 1.126627526124239E-4 2.253255052248478E-5 1.126627526124239E-5 5 1.239290278736663E-4 2.253255052248478E-5 3.379882578372717E-5 6 1.464615783961511E-4 2.253255052248478E-5 5.633137630621195E-5 7 2.1405922996360543E-4 2.253255052248478E-5 5.633137630621195E-5 8 2.7039060626981735E-4 2.253255052248478E-5 6.759765156745434E-5 9 3.0418943205354454E-4 3.379882578372717E-5 6.759765156745434E-5 10-11 3.605208083597565E-4 3.379882578372717E-5 7.886392682869674E-5 12-13 4.506510104496956E-4 3.943196341434836E-5 7.886392682869674E-5 14-15 5.238817996477711E-4 7.323078919807554E-5 7.886392682869674E-5 16-17 6.196451393683315E-4 7.886392682869674E-5 9.013020208993914E-5 18-19 7.097753414582706E-4 7.886392682869674E-5 1.126627526124239E-4 20-21 7.999055435482098E-4 7.886392682869674E-5 1.295621655042875E-4 22-23 8.67503195115664E-4 7.886392682869674E-5 1.6899412891863586E-4 24-25 9.914322229893303E-4 9.013020208993912E-5 1.7462726654925707E-4 26-27 0.0011209943884936179 9.013020208993912E-5 1.8026040417987824E-4 28-29 0.0013463198937184657 1.0139647735118152E-4 1.8026040417987824E-4 30-31 0.001701207564447601 1.0139647735118152E-4 1.8026040417987824E-4 32-33 0.002275787602770963 1.0702961498180271E-4 2.196923675942266E-4 34-35 0.0030418943205354453 1.239290278736663E-4 2.3095864285546902E-4 36-37 0.004309350287425214 1.239290278736663E-4 2.365917804860902E-4 38-39 0.006089421778701512 1.3519530313490868E-4 2.59124331008575E-4 40-41 0.008821493529552792 1.3519530313490868E-4 2.6475746863919615E-4 42-43 0.012528098090501538 1.4082844076552988E-4 2.7602374390043856E-4 44-45 0.017034608194998496 1.520947160267723E-4 2.8165688153105977E-4 46-47 0.023343722341294233 1.6336099128801465E-4 3.041894320535446E-4 48-49 0.03255953550499051 1.6899412891863586E-4 3.379882578372717E-4 50-51 0.04454685238295241 1.6899412891863586E-4 3.379882578372717E-4 52-53 0.05782415777832657 1.8026040417987824E-4 3.605208083597565E-4 54-55 0.07376593727298456 1.9152667944112063E-4 3.605208083597565E-4 56-57 0.09265384774845742 1.9152667944112063E-4 3.7742022125162005E-4 58-59 0.11928168932840381 2.0279295470236304E-4 4.055859094047261E-4 60-61 0.15387478751804856 2.253255052248478E-4 4.1685218466596844E-4 62-63 0.18989306952824048 2.253255052248478E-4 4.2811845992721086E-4 64-65 0.2294151631446788 2.365917804860902E-4 4.2811845992721086E-4 66-67 0.2771391051513016 2.59124331008575E-4 4.393847351884532E-4 68-69 0.33735734642264215 2.7602374390043856E-4 4.450178728190744E-4 70-71 0.4144524680353238 2.8165688153105977E-4 4.6191728571093804E-4 72-73 0.4888380504476767 2.8165688153105977E-4 4.788166986028016E-4 74-75 0.5671048646875276 2.8165688153105977E-4 4.844498362334228E-4 76-77 0.6526046276450961 2.8165688153105977E-4 4.957161114946652E-4 78-79 0.7586709760920627 3.267219825760293E-4 5.126155243865288E-4 80-81 0.9012851214864994 3.4925453309851413E-4 5.295149372783924E-4 82-83 1.0473749127990295 3.5488767072913534E-4 5.407812125396347E-4 84-85 1.1842995891865389 3.7178708362099885E-4 5.745800383233619E-4 86-87 1.3347550621628004 3.8305335888224126E-4 5.802131759539832E-4 88-89 1.5074501625047545 3.9431963414348367E-4 5.971125888458468E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTAG 6290 0.0 6.7046223 94-95 TCGTGTA 6775 0.0 6.4923873 94-95 GTCGTGT 8100 0.0 6.102158 94-95 GCGTCGT 12620 0.0 5.3539596 92-93 CGTCGTG 12360 0.0 5.3381734 92-93 TCGGAAG 19890 0.0 5.323461 94-95 CGGAAGA 19635 0.0 5.3117657 94-95 AGCGTCG 13020 0.0 5.093305 90-91 >>END_MODULE