FastQCFastQC Report
Thu 11 Sep 2014
NYCLib22_CGTACG_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib22_CGTACG_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8876048
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG62900.06.704622394-95
TCGTGTA67750.06.492387394-95
GTCGTGT81000.06.10215894-95
GCGTCGT126200.05.353959692-93
CGTCGTG123600.05.338173492-93
TCGGAAG198900.05.32346194-95
CGGAAGA196350.05.311765794-95
AGCGTCG130200.05.09330590-91
Produced by FastQC (version 0.11.2)