##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib22_CGTACG_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8876048 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23817322754451 33.0 33.0 33.0 27.0 33.0 2 31.672414344762444 33.0 33.0 33.0 27.0 33.0 3 31.82090385270562 33.0 33.0 33.0 27.0 33.0 4 35.64243016711942 37.0 37.0 37.0 33.0 37.0 5 35.13598653364651 37.0 37.0 37.0 33.0 37.0 6 34.94592446998934 37.0 33.0 37.0 33.0 37.0 7 34.75084328070331 37.0 33.0 37.0 27.0 37.0 8 34.80092885933019 37.0 33.0 37.0 27.0 37.0 9 34.706908187067036 37.0 33.0 37.0 27.0 37.0 10-11 34.561735132572515 37.0 33.0 37.0 27.0 37.0 12-13 34.56939360850685 37.0 33.0 37.0 27.0 37.0 14-15 35.50953290248093 37.0 33.0 40.0 27.0 40.0 16-17 35.43137007596174 37.0 33.0 40.0 27.0 40.0 18-19 35.649852670918406 37.0 33.0 40.0 27.0 40.0 20-21 35.34687729268701 37.0 33.0 40.0 27.0 40.0 22-23 35.38668464839307 37.0 33.0 40.0 27.0 40.0 24-25 35.24719137390875 37.0 33.0 40.0 27.0 40.0 26-27 34.894212773522625 37.0 33.0 40.0 27.0 40.0 28-29 34.55145178349644 37.0 33.0 40.0 27.0 40.0 30-31 34.462745751262275 37.0 33.0 40.0 27.0 40.0 32-33 34.489598298702305 37.0 33.0 40.0 27.0 40.0 34-35 34.375705043505846 37.0 33.0 40.0 24.5 40.0 36-37 34.104038813219574 37.0 33.0 38.5 22.0 40.0 38-39 34.04194428646623 37.0 33.0 37.0 24.5 40.0 40-41 33.779671087853515 37.0 33.0 37.0 22.0 40.0 42-43 33.445623266120236 37.0 33.0 37.0 22.0 40.0 44-45 33.37138346930976 37.0 33.0 37.0 22.0 40.0 46-47 33.259021920566454 37.0 33.0 37.0 22.0 40.0 48-49 33.294095750721496 37.0 33.0 37.0 22.0 40.0 50-51 33.57249769266683 37.0 33.0 37.0 22.0 40.0 52-53 33.44103287859642 37.0 33.0 37.0 22.0 40.0 54-55 33.10518994489439 37.0 33.0 37.0 22.0 40.0 56-57 33.1677072949583 37.0 33.0 37.0 22.0 40.0 58-59 32.89070817327712 37.0 33.0 37.0 22.0 40.0 60-61 32.80589030162973 37.0 33.0 37.0 22.0 40.0 62-63 32.79539886444958 37.0 33.0 37.0 22.0 40.0 64-65 32.472572534533384 37.0 33.0 37.0 22.0 40.0 66-67 32.13976698864179 35.0 27.0 37.0 22.0 40.0 68-69 31.976571836925622 33.0 27.0 37.0 22.0 40.0 70-71 31.82176837033779 33.0 27.0 37.0 22.0 40.0 72-73 31.60571692491974 33.0 27.0 37.0 22.0 40.0 74-75 31.259022709205716 33.0 27.0 37.0 15.0 37.0 76-77 30.193567903192953 33.0 27.0 37.0 15.0 37.0 78-79 31.09244694260328 33.0 27.0 37.0 22.0 37.0 80-81 30.783827329460138 33.0 27.0 37.0 15.0 37.0 82-83 30.75574675801663 33.0 27.0 37.0 15.0 37.0 84-85 30.627566232179007 33.0 27.0 37.0 18.5 37.0 86-87 30.42303359558218 33.0 27.0 37.0 15.0 37.0 88-89 30.280189111190026 33.0 27.0 37.0 15.0 37.0 90-91 30.186544338200967 33.0 27.0 37.0 15.0 37.0 92-93 29.973300617572143 33.0 27.0 37.0 15.0 37.0 94-95 29.884125682961606 33.0 27.0 37.0 15.0 37.0 96-97 29.749889703165195 33.0 27.0 33.0 15.0 37.0 98-99 29.49237729448962 33.0 27.0 33.0 15.0 37.0 100-101 28.70016019516794 33.0 27.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.124910621680005 2306 2 0.5312965155848275 2306 3 0.46341025026343274 2306 4 0.37069593183299787 2306 5 0.7009798526253022 2306 6 0.7463999408460253 2306 7 0.8111189121765534 2306 8 0.8033672089383614 2306 9 0.9877356507073998 2306 10-11 1.1394872593963754 2306 12-13 1.361279689838618 2306 14-15 1.494173425507924 2306 16-17 1.4261486496453628 2306 18-19 1.2948635967616227 2306 20-21 1.5662837968537815 2306 22-23 1.232501237576244 2306 24-25 1.2209227433592602 2306 26-27 1.3744644298049735 2306 28-29 1.6015840120153726 2306 30-31 1.5301056319580937 2306 32-33 1.2412260519447287 2306 34-35 1.1902799929364107 2306 36-37 1.3262182797674313 2306 38-39 1.1297778637918086 2306 40-41 1.3449391700715694 2306 42-43 1.2779261143333684 2306 44-45 1.119228873482335 2306 46-47 1.0211790927927638 2306 48-49 1.1055295265012148 2306 50-51 1.132955640288209 2306 52-53 1.2456638047834119 2306 54-55 1.6481644779227267 2306 56-57 1.304691233954614 2306 58-59 1.1656626985945095 2306 60-61 0.9980526979998032 2306 62-63 0.7989541071169697 2306 64-65 0.8731674347672183 2306 66-67 1.0565200082254727 2306 68-69 0.8672698702091033 2306 70-71 0.8165162898202176 2306 72-73 0.591785782409378 2306 74-75 0.8568457063042736 2306 76-77 0.9039184596308161 2306 78-79 0.8676335157907715 2306 80-81 1.3481826052734291 2306 82-83 1.1918690624727404 2306 84-85 0.9220274546890046 2306 86-87 0.8322092130673653 2306 88-89 0.6584507231426073 2306 90-91 0.3944250810114802 2306 92-93 0.26169376353722384 2306 94-95 0.16882759170286477 2306 96-97 0.26843332127791797 2306 98-99 0.4475077932533651 2306 100-101 0.4110638145956038 2307 1 0.6167517273303851 2307 2 0.444360677903763 2307 3 0.37801457152178486 2307 4 0.33739543443596176 2307 5 0.5369476641538995 2307 6 0.5716995500271125 2307 7 0.8022734703881014 2307 8 0.699538286693695 2307 9 0.9781132770167957 2307 10-11 1.1395735946335748 2307 12-13 1.339354840455762 2307 14-15 1.2372603980795134 2307 16-17 1.304362245644107 2307 18-19 1.1869746536160761 2307 20-21 1.424347903866213 2307 22-23 1.1934437093102446 2307 24-25 1.2475296183385751 2307 26-27 1.3369884970629613 2307 28-29 1.5898688743397358 2307 30-31 1.6015083311297502 2307 32-33 1.3346519941234831 2307 34-35 1.3045630239985968 2307 36-37 1.4037000389575454 2307 38-39 1.0770190274090297 2307 40-41 1.1956994418366875 2307 42-43 1.2656120267106772 2307 44-45 1.0392842377812954 2307 46-47 1.0346920138097317 2307 48-49 1.1410705068450255 2307 50-51 1.0601812018434984 2307 52-53 1.2939915837095342 2307 54-55 1.5599375405018279 2307 56-57 1.2219304298075855 2307 58-59 1.2305458623078778 2307 60-61 1.0307609138879883 2307 62-63 0.8901130062396163 2307 64-65 0.9642507988438282 2307 66-67 1.1094374936394438 2307 68-69 0.9069194012868032 2307 70-71 0.8817048014408222 2307 72-73 0.8164133274744927 2307 74-75 0.9246546936175228 2307 76-77 1.005845943486456 2307 78-79 1.0476594588158967 2307 80-81 1.626514653515244 2307 82-83 1.308421203820405 2307 84-85 1.1086298198613314 2307 86-87 1.1153740390665057 2307 88-89 0.983989343543854 2307 90-91 0.8048792661324988 2307 92-93 0.8642693765643514 2307 94-95 0.709634562883025 2307 96-97 0.6301790014558009 2307 98-99 0.7182469905349969 2307 100-101 0.8174265144482469 1101 1 1.0068688214877568 1101 2 0.5193761220288842 1101 3 0.39631403059722103 1101 4 0.31381549053694613 1101 5 0.557441329551942 1101 6 0.5693788066087606 1101 7 0.700492238393096 1101 8 0.6498049040845402 1101 9 0.8304769454907515 1101 10-11 0.9698207451099989 1101 12-13 1.1718564350814162 1101 14-15 1.3072480011742584 1101 16-17 1.229859925940815 1101 18-19 0.9489320325651178 1101 20-21 1.3045667341354914 1101 22-23 1.0122299093926443 1101 24-25 1.0801089177878964 1101 26-27 1.120415917852462 1101 28-29 1.3253469313439794 1101 30-31 1.297860870651789 1101 32-33 0.8574427489674719 1101 34-35 1.034159329775349 1101 36-37 1.0989837381389833 1101 38-39 0.8864303817174886 1101 40-41 1.0867310426830201 1101 42-43 1.121922286057206 1101 44-45 0.9801825371055131 1101 46-47 0.8849126574904531 1101 48-49 0.8887434688517573 1101 50-51 0.8466038360302051 1101 52-53 1.1455273631493554 1101 54-55 1.5047098368545804 1101 56-57 1.1707826329016342 1101 58-59 1.1723733018627414 1101 60-61 0.9029347035506277 1101 62-63 0.7237822893452801 1101 64-65 0.7727585319532793 1101 66-67 0.8300517817434603 1101 68-69 0.8161472911530474 1101 70-71 0.877843752227367 1101 72-73 0.733492453186372 1101 74-75 0.8134663897769947 1101 76-77 0.8931103757452092 1101 78-79 0.8886518073386505 1101 80-81 1.4304249797456663 1101 82-83 1.2118774168204958 1101 84-85 1.0784725473067418 1101 86-87 0.8456005734650844 1101 88-89 0.7923626130558183 1101 90-91 0.6621050722971802 1101 92-93 0.7312287834066495 1101 94-95 0.7312837759427993 1101 96-97 0.747857783164676 1101 98-99 0.7370739923748033 1101 100-101 0.7455168602130158 2201 1 1.451932491798594 2201 2 0.7927283961979548 2201 3 0.33950285102557487 2201 4 0.254685237701608 2201 5 0.37770535387578263 2201 6 0.4857874365440509 2201 7 0.5925504283463994 2201 8 0.5797436623071945 2201 9 0.5608633297949268 2201 10-11 0.7727075604926341 2201 12-13 1.0150461969106672 2201 14-15 1.0713496939050486 2201 16-17 0.9917296050294482 2201 18-19 0.8665308765765687 2201 20-21 1.1532613632414908 2201 22-23 0.9220871420764212 2201 24-25 0.8415579773539932 2201 26-27 1.0131730656267735 2201 28-29 1.2969287982652773 2201 30-31 1.3467561241585173 2201 32-33 0.9733931149083688 2201 34-35 0.9823275401045422 2201 36-37 1.0413653010937978 2201 38-39 0.6975460432848166 2201 40-41 1.0518385935101904 2201 42-43 0.9513018562708737 2201 44-45 0.9358276871600211 2201 46-47 0.8715040219601704 2201 48-49 0.9566811081537665 2201 50-51 0.8317560934055024 2201 52-53 1.052079962001784 2201 54-55 1.4004686217358113 2201 56-57 1.018120134439151 2201 58-59 1.0905728761237512 2201 60-61 0.9004074923009426 2201 62-63 0.7133657309805628 2201 64-65 0.7633865376076514 2201 66-67 0.8440123207836159 2201 68-69 0.8202621988507168 2201 70-71 0.8729000276261907 2201 72-73 0.693014616533695 2201 74-75 0.7688872322730376 2201 76-77 0.7729481259733717 2201 78-79 0.8400144974052672 2201 80-81 1.2979393088642261 2201 82-83 1.1500266888287598 2201 84-85 0.9741211244019858 2201 86-87 0.9848476863407676 2201 88-89 0.9543252947894239 2201 90-91 0.8407732366659673 2201 92-93 0.9553125505865694 2201 94-95 0.8258021259418591 2201 96-97 0.6345832605053232 2201 98-99 0.7882957740822221 2201 100-101 0.9260723998598088 2304 1 -1.9270725994521989 2304 2 -0.27499305391032536 2304 3 -0.13749794335455334 2304 4 0.028019297283272238 2304 5 0.41122094439086254 2304 6 0.5154138707876754 2304 7 0.5040832817950474 2304 8 0.614315289235634 2304 9 0.8419644471904988 2304 10-11 0.8427582729631169 2304 12-13 1.138202639185188 2304 14-15 1.1962823302193826 2304 16-17 1.1541887160362734 2304 18-19 0.8509753191261069 2304 20-21 1.121485789478605 2304 22-23 0.8889136002672302 2304 24-25 0.9699577010017393 2304 26-27 1.054817736704095 2304 28-29 1.4378270671069302 2304 30-31 1.454275379434236 2304 32-33 0.9252917730158146 2304 34-35 0.8721996986493536 2304 36-37 1.1702908814671105 2304 38-39 0.8972534846331115 2304 40-41 0.9150778538156388 2304 42-43 0.9390429559023943 2304 44-45 0.9145634921133947 2304 46-47 0.9650423264439993 2304 48-49 0.8787661271351155 2304 50-51 0.8033459981431719 2304 52-53 0.9931166760671175 2304 54-55 1.4038985305458098 2304 56-57 1.0540285753995988 2304 58-59 0.8573175321273183 2304 60-61 0.5148590368448467 2304 62-63 0.30331816626959807 2304 64-65 0.38642167833140917 2304 66-67 0.5137895917407711 2304 68-69 0.5675552635336238 2304 70-71 0.47252773460999364 2304 72-73 0.36080771248906274 2304 74-75 0.598564321009821 2304 76-77 0.6648349054753169 2304 78-79 0.5771437974111286 2304 80-81 1.2077373334648023 2304 82-83 0.9636785112248525 2304 84-85 0.5802558453150901 2304 86-87 0.47369305643933046 2304 88-89 0.31060524818656177 2304 90-91 0.2305465244869751 2304 92-93 0.32085005766035124 2304 94-95 0.20773988749657946 2304 96-97 0.1066319758651133 2304 98-99 0.5220533208271014 2304 100-101 0.533905847469736 1102 1 1.4652968381896443 1102 2 0.15614368312209947 1102 3 0.3007481617501462 1102 4 0.17015282151751876 1102 5 0.2883102663966426 1102 6 0.13493571152699957 1102 7 -4.639232329183901 1102 8 -1.1522243549999303 1102 9 0.17750698683510535 1102 10-11 0.6610804015311942 1102 12-13 0.9099332235070463 1102 14-15 0.7779821841358654 1102 16-17 0.5465814042324695 1102 18-19 0.8060976292883524 1102 20-21 1.0794653609487952 1102 22-23 0.62088083810184 1102 24-25 0.9256606803091856 1102 26-27 1.0063133751231135 1102 28-29 0.8961393670191384 1102 30-31 1.1693467925386472 1102 32-33 0.9646977926201359 1102 34-35 1.1245512227416725 1102 36-37 1.2478966854377518 1102 38-39 0.8866204965173381 1102 40-41 1.139218597056491 1102 42-43 1.0782652916279218 1102 44-45 1.0427230927921514 1102 46-47 0.9016642399637291 1102 48-49 0.9413836280224075 1102 50-51 0.7631229206066052 1102 52-53 1.0121791433676606 1102 54-55 1.3302620353895733 1102 56-57 1.05647474695256 1102 58-59 0.9882286838107532 1102 60-61 0.9023300551315572 1102 62-63 0.7156749991961107 1102 64-65 0.4456594465561423 1102 66-67 0.6406559236171923 1102 68-69 0.766343304961854 1102 70-71 0.5647408670990224 1102 72-73 0.5097765456110182 1102 74-75 0.5937340765447452 1102 76-77 0.5979023266757153 1102 78-79 0.5988544960043463 1102 80-81 1.2141794937699188 1102 82-83 1.0833097987890206 1102 84-85 0.7309819664851851 1102 86-87 0.604952105668545 1102 88-89 0.5190108731046514 1102 90-91 0.12072961576517827 1102 92-93 0.2759801401787101 1102 94-95 0.16727087250188433 1102 96-97 0.03939963022132531 1102 98-99 0.13255269054047147 1102 100-101 0.31583048230710986 2202 1 1.5197251038838928 2202 2 0.9308600941048546 2202 3 0.3948316621817973 2202 4 -0.33394124414643755 2202 5 0.10886324866124397 2202 6 0.5047512002585961 2202 7 0.6032553844143109 2202 8 -3.823799597327678 2202 9 -0.36888485934335336 2202 10-11 0.5460535923856398 2202 12-13 0.03180031439709552 2202 14-15 0.11911245849925223 2202 16-17 0.2654593294788299 2202 18-19 0.7721508312366794 2202 20-21 1.1997515326951813 2202 22-23 -2.631361094731055 2202 24-25 -2.8229987967130015 2202 26-27 -1.3743736009874965 2202 28-29 0.6003115211162893 2202 30-31 1.2778021705477514 2202 32-33 1.065334116263145 2202 34-35 -1.9928709773963185 2202 36-37 -0.24769001913448818 2202 38-39 0.2395734474934983 2202 40-41 1.2202590355789127 2202 42-43 1.2446544119784875 2202 44-45 1.2948338990950035 2202 46-47 0.6281066640085484 2202 48-49 0.9317309682149215 2202 50-51 -3.8473374549740598 2202 52-53 -0.24375642233004413 2202 54-55 1.4926712681350622 2202 56-57 1.2963542758544904 2202 58-59 1.3146925879287537 2202 60-61 1.3048036477306795 2202 62-63 1.1558583510085185 2202 64-65 1.1611813562138238 2202 66-67 1.3139902040472826 2202 68-69 1.2222989905315664 2202 70-71 1.224697941964397 2202 72-73 1.0554929347050859 2202 74-75 0.7026197342105363 2202 76-77 1.3098615117052255 2202 78-79 1.296747212915026 2202 80-81 1.8941295846020267 2202 82-83 1.600212326628597 2202 84-85 1.4459847934486412 2202 86-87 1.4738214386313118 2202 88-89 1.4357896069539464 2202 90-91 1.1957304897866265 2202 92-93 1.2497146660570877 2202 94-95 1.176680249583832 2202 96-97 1.0730540825103176 2202 98-99 1.2546654272353948 2202 100-101 1.1870254858850906 2305 1 1.3328293371202022 2305 2 0.7027537958510628 2305 3 0.15368170589769647 2305 4 -0.19807639518467823 2305 5 0.2774747964068567 2305 6 0.34997760071021844 2305 7 0.5179111139185224 2305 8 -0.10023668719792767 2305 9 0.4821614404640826 2305 10-11 0.26147027515111176 2305 12-13 0.8412166105581989 2305 14-15 0.5268164481060396 2305 16-17 0.5990853987817104 2305 18-19 0.6447381888773691 2305 20-21 0.814624577098563 2305 22-23 0.5731146376275262 2305 24-25 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-0.5625892161289983 1208 40-41 -0.8792378373235081 1208 42-43 -0.0025045805985826064 1208 44-45 -0.059476038566785405 1208 46-47 -0.14162120708523673 1208 48-49 -1.7153754012980649 1208 50-51 -0.34612297862297936 1208 52-53 -1.6581791398960313 1208 54-55 -1.0284373662207145 1208 56-57 -6.330604103373759 1208 58-59 -2.1831401644341284 1208 60-61 -1.5323226838790163 1208 62-63 0.1568712019129208 1208 64-65 -0.15935703110243793 1208 66-67 0.6470816169665632 1208 68-69 0.2587494100045049 1208 70-71 -4.200247471567398 1208 72-73 -0.8336425343737908 1208 74-75 -5.72484729812577 1208 76-77 -1.3137644203229826 1208 78-79 -4.163603593455324 1208 80-81 0.7935533871062432 1208 82-83 0.12005435995071778 1208 84-85 -3.0479065415259576 1208 86-87 -1.0766421410711757 1208 88-89 -4.735936111350739 1208 90-91 -0.18211099684929621 1208 92-93 -2.319921349488908 1208 94-95 -4.8529016099641495 1208 96-97 -3.662814350573804 1208 98-99 -6.546824571945287 1208 100-101 -3.9777759331768756 1209 1 1.8500262799270502 1209 2 1.2656969622787777 1209 3 0.5486745375028796 1209 4 0.385488283301747 1209 5 0.3973478077473942 1209 6 0.9908132590623353 1209 7 0.8026692690004182 1209 8 0.18269939345825037 1209 9 -0.10264724700910932 1209 10-11 -2.8941826106152817 1209 12-13 -0.24114844416154568 1209 14-15 -1.36541461811089 1209 16-17 -0.39681189322412536 1209 18-19 -16.83531975977855 1209 20-21 -14.203013111274224 1209 22-23 -3.662919848663954 1209 24-25 -2.1286144858149854 1209 26-27 -1.6367579287511305 1209 28-29 -3.1546021629270555 1209 30-31 -0.994009212796712 1209 32-33 -0.7347083113108823 1209 34-35 -3.3192604716613943 1209 36-37 -4.278274924088816 1209 38-39 -1.3854932727215186 1209 40-41 -10.39209051562871 1209 42-43 -5.153679212111101 1209 44-45 -2.8941906409084055 1209 46-47 -2.572100097945352 1209 48-49 -3.2742683610253387 1209 50-51 -2.537307822431991 1209 52-53 -1.2394875934909564 1209 54-55 -3.0175807446272955 1209 56-57 -1.006520135188417 1209 58-59 -1.9557717067642173 1209 60-61 -1.8293943824000962 1209 62-63 -1.0492573251807258 1209 64-65 -0.2478358648134531 1209 66-67 0.09060584694449503 1209 68-69 -1.2447033898046662 1209 70-71 -1.0836325496877386 1209 72-73 -0.6116790153804104 1209 74-75 0.18833662757045389 1209 76-77 -16.893908305617277 1209 78-79 -16.99338625913803 1209 80-81 -22.343558030042463 1209 82-83 -10.306636414941448 1209 84-85 -3.929143776155332 1209 86-87 -2.4115205811987934 1209 88-89 -2.0124292870022416 1209 90-91 -6.283966976136476 1209 92-93 -3.6513183531365137 1209 94-95 -0.9940799313210178 1209 96-97 -0.19586855401467673 1209 98-99 0.9843226528445044 1209 100-101 0.3619713621796343 1212 1 -28.62283435576977 1212 2 -16.319874133562422 1212 3 -5.731752041406878 1212 4 -1.3898045937883694 1212 5 -0.525339593033614 1212 6 -2.5668702984914376 1212 7 -0.3990310210763326 1212 8 -1.2367183672728501 1212 9 0.05336846580332377 1212 10-11 0.7583753564527029 1212 12-13 0.2770109010606916 1212 14-15 -0.23369196392795288 1212 16-17 -0.18140074516138327 1212 18-19 -1.117284402088039 1212 20-21 -0.8596951122244079 1212 22-23 -0.5821483449385312 1212 24-25 0.11405306151078065 1212 26-27 -0.671110586371725 1212 28-29 0.20397286884233523 1212 30-31 -0.7301027171633123 1212 32-33 0.2512552185871044 1212 34-35 -1.3340787198450315 1212 36-37 -1.4891858268802736 1212 38-39 -0.6276598486184923 1212 40-41 -0.4118535874294622 1212 42-43 -0.13549360161735535 1212 44-45 0.87793533969392 1212 46-47 -2.403672499325051 1212 48-49 -1.7584767527795861 1212 50-51 -0.26171637100535605 1212 52-53 -1.230796106142705 1212 54-55 -0.586257090819025 1212 56-57 -1.6488134023950636 1212 58-59 -0.4363180244117828 1212 60-61 0.004515241890644006 1212 62-63 -4.877571287876314 1212 64-65 -4.204556104908935 1212 66-67 -1.7003345656520175 1212 68-69 0.13729524285619732 1212 70-71 -0.8931340116591038 1212 72-73 -1.3000353887644174 1212 74-75 -0.10662662036212112 1212 76-77 0.8369201705720961 1212 78-79 -1.338925991155012 1212 80-81 0.5252874953981568 1212 82-83 0.647466954352133 1212 84-85 -3.2281522621555574 1212 86-87 -1.9062505317196567 1212 88-89 -0.9220134073280839 1212 90-91 -0.2319609660243458 1212 92-93 -2.5888104832114074 1212 94-95 -2.152452179744138 1212 96-97 -0.03631398211928882 1212 98-99 -0.6581119833235434 1212 100-101 0.01659231182370391 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 27.0 10 42.0 11 63.0 12 172.0 13 648.0 14 2045.0 15 4707.0 16 9050.0 17 15268.0 18 22979.0 19 34005.0 20 48217.0 21 66020.0 22 88609.0 23 115992.0 24 148626.0 25 187161.0 26 234093.0 27 286171.0 28 347464.0 29 418393.0 30 496737.0 31 584959.0 32 682502.0 33 786353.0 34 891413.0 35 985942.0 36 1052518.0 37 989982.0 38 374664.0 39 1220.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.93577142071276 29.569070663623986 20.45224483330396 22.042913082359288 2 22.21392375085598 30.045985724325856 26.1934194536649 21.546671071153263 3 19.129022380060626 28.188222972758304 31.66694194000784 21.015812707173232 4 22.13291191436185 27.09631678256665 30.007365894085247 20.763405408986255 5 21.090264496091052 29.807635109679442 28.309006440704245 20.79309395352526 6 20.98396718900123 29.481893293051144 28.43531265265803 21.098826865289595 7 20.926238794562625 29.497959001573676 29.545085830991454 20.030716372872252 8 20.108893056910013 28.142986608454574 30.50902834234335 21.239091992292064 9 21.025438348237866 27.80363513131069 29.554786093991382 21.616140426460063 10-11 21.40335428560098 28.108004823768418 28.739856972382306 21.7487839182483 12-13 21.294297956111727 28.28820159448514 28.71188504084418 21.705615408558952 14-15 21.109627968396293 28.636801945731023 28.72036391875798 21.533206167114706 16-17 21.24268315785756 28.600323467359186 28.69448175005181 21.462511624731444 18-19 21.67335427642044 28.466303588739322 28.289443667831126 21.570898467009112 20-21 21.499245187595626 28.38954257393762 28.35365948116653 21.757552757300225 22-23 21.199987945084793 28.19681846001838 28.794336668432656 21.808856926464173 24-25 21.3325513787217 28.1185613236882 28.801596160813915 21.747291136776187 26-27 21.408379044367493 28.47477278176053 28.54903443514501 21.567813738726965 28-29 21.75375234563851 28.30010045011023 28.405806277748834 21.540340926502424 30-31 21.729755179332063 28.190265532588377 28.198213889785183 21.881765398294377 32-33 21.382207486935627 28.23621503624135 28.53534027756497 21.84623719925805 34-35 21.59053781592889 28.208308472419258 28.550482151516082 21.650671560135773 36-37 21.607020376636086 28.401496927461412 28.44438200424333 21.54710069165917 38-39 21.58473005103172 28.29715995226704 28.565145208768588 21.552964787932645 40-41 21.815649321832076 28.154224152392903 28.295883042382524 21.734243483392497 42-43 21.42949204420706 28.131055622952918 28.66338712904662 21.776065203793397 44-45 21.413910785520763 28.076752176193732 28.753207508566874 21.75612952971863 46-47 21.53030359033628 28.370766194737573 28.602253206357673 21.49667700856847 48-49 21.69714590161404 28.220452514707283 28.53429732145281 21.54810426222586 50-51 21.749343809473647 28.2400912343536 28.314797951902555 21.695767004270202 52-53 21.464049778071 28.224516188925303 28.680485446376576 21.630948586627117 54-55 21.47554598552128 27.988502739483025 28.75174201557067 21.784209259425023 56-57 21.536721134610886 28.266842978826766 28.535637529118098 21.66079835744425 58-59 21.546759429928603 28.21593260221406 28.66215864037933 21.575149327478005 60-61 21.794180714275456 28.02370571831025 28.38969041792018 21.792423149494123 62-63 21.559537986441985 28.075390409793677 28.54831550046023 21.816756103304105 64-65 21.5774020770945 28.090815988535194 28.61121063198383 21.720571302386475 66-67 21.685895330966982 28.132324261522907 28.506143443745703 21.675636963764415 68-69 21.70619145101023 28.18622467906094 28.473719650546634 21.633864219382197 70-71 21.801347299241478 28.217104649051606 28.342344677887453 21.63920337381946 72-73 21.54513997290098 28.12212952032573 28.564793411119975 21.767937095653313 74-75 21.531831251123958 28.042624375620377 28.672474795021902 21.75306957823376 76-77 21.576459233682595 28.361412823773808 28.55893697039406 21.50319097214954 78-79 21.895825416405522 28.231320491170425 28.25390943669254 21.618944655731514 80-81 21.79689009508923 28.152025232602195 28.255610149393057 21.79547452291552 82-83 21.702523722463877 28.204209963451728 28.338673218518196 21.7545930955662 84-85 21.66482905104673 27.97750627786281 28.438955107647544 21.918709563442917 86-87 21.58321047149595 28.29524405118391 28.436813787461407 21.684731689858733 88-89 21.720441859161546 28.284456482641307 28.347320883076105 21.647780775121042 90-91 21.879137046708145 28.098054965447698 28.086397424229926 21.936410563614228 92-93 21.633479402777418 28.087541160727298 28.274980953771138 22.003998482724143 94-95 21.688629668359717 28.132129904956294 28.198401735067836 21.980838691616153 96-97 21.720349915274927 28.228563779629408 28.159511635655264 21.891574669440395 98-99 21.73594269636772 28.259969997880006 28.150894427005085 21.853192878747187 100-101 21.89512720112235 28.156512462420963 27.902460641798875 22.045899694657813 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 5.0 2 9.5 3 13.0 4 18.5 5 37.5 6 67.0 7 110.0 8 163.5 9 270.0 10 444.0 11 682.5 12 1035.0 13 1624.5 14 2446.0 15 3596.5 16 5351.0 17 7940.0 18 11867.5 19 17500.5 20 24800.0 21 34340.0 22 46445.0 23 60462.0 24 76362.5 25 94794.0 26 115556.0 27 137353.0 28 159298.0 29 181275.5 30 202740.0 31 223299.0 32 242799.0 33 261084.0 34 278035.0 35 293929.0 36 308122.5 37 317810.0 38 321555.5 39 318605.5 40 309503.5 41 296712.5 42 280246.5 43 261437.0 44 243079.5 45 227505.0 46 214638.5 47 203357.5 48 194305.5 49 184661.5 50 174704.5 51 165787.0 52 157586.5 53 151432.0 54 147792.5 55 146066.0 56 145509.5 57 145704.0 58 145111.5 59 142966.0 60 138646.5 61 132430.0 62 124657.0 63 115265.0 64 105020.0 65 94290.5 66 83074.5 67 71825.0 68 61501.0 69 51985.0 70 43037.5 71 35360.5 72 29097.0 73 23526.5 74 18756.5 75 14915.5 76 11552.0 77 8742.0 78 6510.5 79 4743.0 80 3410.0 81 2409.0 82 1669.5 83 1147.5 84 784.5 85 540.0 86 386.5 87 262.5 88 172.0 89 124.5 90 90.5 91 59.5 92 41.0 93 25.0 94 17.5 95 9.5 96 4.0 97 3.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7121525255383928 2 0.002816568815310598 3 2.478580557473326E-4 4 4.506510104496957E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.19237728322334446 14-15 1.1266275261242392E-5 16-17 4.1121904703534724E-4 18-19 0.11575534517163494 20-21 1.6899412891863588E-5 22-23 5.633137630621196E-6 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0016786750139251162 42-43 0.0 44-45 0.0 46-47 0.023839438452788898 48-49 5.633137630621195E-5 50-51 6.196451393683315E-5 52-53 1.239290278736663E-4 54-55 0.002191290538311645 56-57 0.0013970181323940565 58-59 0.0952788898843269 60-61 0.001470248921592132 62-63 0.001329420480826602 64-65 0.002118059749113569 66-67 0.004129089883245336 68-69 0.004669871095784971 70-71 0.003284119238652157 72-73 0.038327868438746616 74-75 0.04440602394218688 76-77 0.15878125039431962 78-79 2.8165688153105977E-4 80-81 0.1168876058353898 82-83 0.00438258107662329 84-85 0.004686770508676835 86-87 0.0063485461097100866 88-89 0.006709066918069844 90-91 0.022126964613080054 92-93 0.010494535405847287 94-95 0.013930749360526216 96-97 0.009812925752542122 98-99 0.011362038600962951 100-101 0.012246441208970478 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8876048.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.05224632267439 #Duplication Level Percentage of deduplicated Percentage of total 1 97.62762002235242 93.77352206283453 2 1.7638953295228528 3.3885221735748794 3 0.25178855410257656 0.7255456865967213 4 0.13029415857946672 0.5006018645712216 5 0.05996232285406936 0.2879757902429399 6 0.052164644235688555 0.3006318754476621 7 0.03914110891125347 0.263171400414044 8 0.026105826623018427 0.20060186314009576 9 0.015960745878207748 0.13797589451284986 >10 0.03304801383337643 0.4021986462838224 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 1.9273107083216473E-5 0.01925274238118805 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0139647735118152E-4 0.0 0.0 2 1.126627526124239E-4 1.126627526124239E-5 0.0 3 1.126627526124239E-4 3.379882578372717E-5 0.0 4 1.126627526124239E-4 3.379882578372717E-5 1.126627526124239E-5 5 1.3519530313490868E-4 3.379882578372717E-5 3.379882578372717E-5 6 1.5772785365739347E-4 3.379882578372717E-5 5.633137630621195E-5 7 2.478580557473326E-4 3.379882578372717E-5 5.633137630621195E-5 8 3.379882578372717E-4 4.506510104496956E-5 5.633137630621195E-5 9 4.055859094047261E-4 4.506510104496956E-5 6.759765156745434E-5 10-11 4.6755042334155924E-4 5.069823867559076E-5 7.323078919807554E-5 12-13 5.464143501702559E-4 5.633137630621195E-5 7.886392682869674E-5 14-15 6.478108275214374E-4 5.633137630621195E-5 1.0139647735118152E-4 16-17 7.492073048726189E-4 5.633137630621195E-5 1.0139647735118152E-4 18-19 8.449706445931793E-4 5.633137630621195E-5 1.126627526124239E-4 20-21 9.576333972056031E-4 5.633137630621195E-5 1.3519530313490868E-4 22-23 0.0010364973240343 5.633137630621195E-5 1.5772785365739347E-4 24-25 0.0011604263519079663 6.759765156745434E-5 1.8589354181049942E-4 26-27 0.0013406867560878445 7.323078919807554E-5 1.9715981707174184E-4 28-29 0.0016617756010332526 7.886392682869674E-5 2.196923675942266E-4 30-31 0.0021236928867441906 7.886392682869674E-5 2.365917804860902E-4 32-33 0.002822201952941219 7.886392682869674E-5 2.534911933779538E-4 34-35 0.003717870836209989 7.886392682869674E-5 2.8165688153105977E-4 36-37 0.005058557592297834 7.886392682869674E-5 2.8165688153105977E-4 38-39 0.007204783029564508 8.449706445931793E-5 3.0418943205354454E-4 40-41 0.0101790796985325 9.013020208993912E-5 3.267219825760293E-4 42-43 0.014111009764706094 9.576333972056032E-5 3.436213954678929E-4 44-45 0.01923716500857138 1.0139647735118152E-4 3.4925453309851413E-4 46-47 0.02604762840399241 1.0139647735118152E-4 3.605208083597565E-4 48-49 0.03593941808336323 1.126627526124239E-4 3.661539459903777E-4 50-51 0.04925615544215173 1.126627526124239E-4 4.055859094047261E-4 52-53 0.06337843148211907 1.126627526124239E-4 4.1685218466596844E-4 54-55 0.08048063732868502 1.239290278736663E-4 4.6191728571093804E-4 56-57 0.10095145947836244 1.239290278736663E-4 4.6191728571093804E-4 58-59 0.12821584561056903 1.239290278736663E-4 4.6191728571093804E-4 60-61 0.16434115723574275 1.239290278736663E-4 4.6191728571093804E-4 62-63 0.20141846912049147 1.239290278736663E-4 4.7318356097218045E-4 64-65 0.24191509554702723 1.3519530313490868E-4 4.90082973864044E-4 66-67 0.2902248838672346 1.4082844076552988E-4 4.957161114946652E-4 68-69 0.35266821450267055 1.464615783961511E-4 5.069823867559076E-4 70-71 0.4327432659219509 1.464615783961511E-4 5.126155243865288E-4 72-73 0.5120803763116197 1.5772785365739347E-4 5.1824866201715E-4 74-75 0.5940706945253114 1.8589354181049942E-4 5.295149372783924E-4 76-77 0.6865217493190663 1.9152667944112063E-4 5.520474878008771E-4 78-79 0.8014321238461081 1.9152667944112063E-4 5.802131759539832E-4 80-81 0.9530592894495388 1.9715981707174184E-4 5.971125888458467E-4 82-83 1.1104998530877705 2.0279295470236304E-4 6.140120017377103E-4 84-85 1.2579866625326948 2.0279295470236304E-4 6.252782769989527E-4 86-87 1.4169932384322392 2.0279295470236304E-4 6.421776898908163E-4 88-89 1.602971277307198 2.0842609233298425E-4 6.478108275214374E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACGA 2480 0.0 7.84603 94-95 GTACGAT 2445 0.0 7.1819215 94-95 ACCGTAC 3150 0.0 6.9307227 92-93 CACGTCT 11815 0.0 6.587611 94-95 ACGTCTG 10850 0.0 6.495529 94-95 CCGTACG 2965 0.0 6.40275 92-93 ACACGTC 12225 0.0 6.366677 94-95 CGTCTGA 9835 0.0 6.152529 94-95 CACACGT 13415 0.0 5.837468 92-93 TACGATC 2280 0.0 5.8282876 94-95 CTCGTAT 1780 0.0 5.7341256 42-43 CGTATGC 1765 0.0 5.648372 44-45 GCACACG 14200 0.0 5.5147634 92-93 TCGGAAG 21045 0.0 5.4686565 94-95 CGGAAGA 20215 0.0 5.399728 94-95 ATCGGAA 22240 0.0 5.270842 94-95 TCGTATG 2125 0.0 5.1382785 42-43 TATGCCG 1850 0.0 5.1322403 46-47 CACCGTA 3900 0.0 5.112386 90-91 >>END_MODULE