##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib21_GTTTCG_L007_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4620382
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.829709318407005	27.0	22.0	33.0	2.0	33.0
2	24.914997071670697	33.0	15.0	33.0	2.0	33.0
3	25.762640621489737	33.0	22.0	33.0	6.0	33.0
4	27.889629255762834	33.0	22.0	37.0	2.0	37.0
5	27.937025769730727	33.0	15.0	37.0	6.0	37.0
6	28.98415931842865	33.0	22.0	37.0	15.0	37.0
7	28.651551971243936	33.0	27.0	37.0	6.0	37.0
8	28.42017348349119	33.0	22.0	37.0	2.0	37.0
9	29.15899551162653	33.0	27.0	37.0	15.0	37.0
10-11	28.79195334931181	33.0	24.5	37.0	10.5	37.0
12-13	29.085859134591036	33.0	27.0	37.0	10.5	37.0
14-15	29.406684014438632	33.0	27.0	37.0	6.0	40.0
16-17	30.249432947319075	33.0	27.0	37.0	15.0	40.0
18-19	30.55082458549964	33.0	27.0	37.0	15.0	40.0
20-21	30.56285497606042	33.0	27.0	37.0	15.0	40.0
22-23	30.579552404974308	33.0	27.0	37.0	15.0	40.0
24-25	30.161170440019895	33.0	27.0	37.0	10.5	40.0
26-27	29.649084751001105	33.0	27.0	37.0	10.5	40.0
28-29	29.825431641799312	33.0	27.0	37.0	15.0	40.0
30-31	29.72202179386899	33.0	27.0	37.0	15.0	40.0
32-33	29.551893328300558	33.0	27.0	37.0	15.0	40.0
34-35	29.577506253811915	33.0	27.0	37.0	15.0	40.0
36-37	29.806106075211964	33.0	27.0	37.0	15.0	40.0
38-39	29.837355006577376	33.0	27.0	37.0	15.0	40.0
40-41	29.902729254853817	33.0	27.0	37.0	15.0	40.0
42-43	29.80251762733038	33.0	27.0	37.0	15.0	40.0
44-45	29.54121747942053	33.0	27.0	37.0	15.0	40.0
46-47	29.154611458533083	33.0	22.0	37.0	10.5	40.0
48-49	28.992826675370132	33.0	22.0	37.0	10.5	40.0
50-51	28.233238398902948	33.0	22.0	37.0	10.5	38.5
52-53	27.952317903584593	33.0	22.0	37.0	6.0	37.0
54-55	28.863182524734967	33.0	22.0	37.0	15.0	40.0
56-57	28.77314884353718	33.0	22.0	37.0	10.5	40.0
58-59	28.964084246713803	33.0	22.0	37.0	15.0	40.0
60-61	28.719288470087537	33.0	22.0	37.0	10.5	40.0
62-63	28.580477761362587	33.0	22.0	37.0	15.0	40.0
64-65	28.534295432715304	33.0	22.0	37.0	10.5	40.0
66-67	28.401048333233053	33.0	22.0	37.0	10.5	40.0
68-69	28.114636625283367	33.0	22.0	37.0	6.0	38.5
70-71	28.00678948190864	33.0	22.0	37.0	6.0	37.0
72-73	27.763115149353453	33.0	22.0	37.0	6.0	37.0
74-75	27.70987020120847	33.0	22.0	37.0	10.5	37.0
76-77	27.309834554805207	30.0	22.0	35.0	6.0	37.0
78-79	26.734412436028016	27.0	22.0	33.0	6.0	37.0
80-81	26.61181207527862	27.0	22.0	33.0	6.0	37.0
82-83	26.18554732054622	27.0	22.0	33.0	6.0	37.0
84-85	26.26226370460278	27.0	22.0	33.0	6.0	37.0
86-87	25.956359777178598	27.0	22.0	33.0	6.0	37.0
88-89	25.5867807034137	27.0	22.0	33.0	6.0	37.0
90-91	25.437983590967153	27.0	22.0	33.0	6.0	37.0
92-93	25.316327091569484	27.0	22.0	33.0	6.0	37.0
94-95	25.083034151721655	27.0	15.0	33.0	6.0	37.0
96-97	24.93093287524711	27.0	15.0	33.0	6.0	37.0
98-99	24.68807070497634	27.0	15.0	33.0	6.0	37.0
100-101	24.197299162709925	27.0	15.0	33.0	6.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	8.205043678540637
2306	2	8.444291190461946
2306	3	7.360465008886205
2306	4	8.914521171017636
2306	5	8.8357773956891
2306	6	7.8947517561172695
2306	7	8.064364178124471
2306	8	8.535197333915804
2306	9	7.393538529571064
2306	10-11	7.699911735407266
2306	12-13	7.3439593864018455
2306	14-15	7.931923567095051
2306	16-17	6.641632334591225
2306	18-19	6.046756668262315
2306	20-21	6.051027809685177
2306	22-23	5.714506015505851
2306	24-25	5.671016398174455
2306	26-27	6.335280361129591
2306	28-29	5.356265786610912
2306	30-31	5.303933739046219
2306	32-33	5.1413038313208475
2306	34-35	4.536211629144571
2306	36-37	4.570460339865857
2306	38-39	4.360791013931127
2306	40-41	3.5976792630253094
2306	42-43	3.2774465762697744
2306	44-45	3.0261017423141574
2306	46-47	3.2369387915779484
2306	48-49	3.099162398469403
2306	50-51	2.9021185470177464
2306	52-53	3.497442473440902
2306	54-55	3.0544838126537996
2306	56-57	3.3908732662159693
2306	58-59	2.9277476454423805
2306	60-61	2.8795207025552294
2306	62-63	2.462830919549962
2306	64-65	2.0952352667424776
2306	66-67	1.925890111616667
2306	68-69	2.156008368140693
2306	70-71	2.260201366610854
2306	72-73	1.4467311128021905
2306	74-75	1.0586951503773854
2306	76-77	1.3860111849500996
2306	78-79	1.7172328785044293
2306	80-81	1.4248118056620243
2306	82-83	1.3623722934278995
2306	84-85	0.9877038176422026
2306	86-87	0.8392268724192462
2306	88-89	0.8402800953359133
2306	90-91	0.7763353567578335
2306	92-93	0.6987244624479736
2306	94-95	0.9605140769982263
2306	96-97	0.7627483029425832
2306	98-99	0.5266639670506592
2306	100-101	0.2688883895487457
2307	1	8.289434040496314
2307	2	8.354400629669616
2307	3	7.533900383325417
2307	4	9.156902686870456
2307	5	9.058373557837655
2307	6	7.528164243059113
2307	7	8.107710325375479
2307	8	8.567133346213275
2307	9	7.571772154734173
2307	10-11	7.839188626696522
2307	12-13	7.364203949405091
2307	14-15	8.280502705907363
2307	16-17	6.883265932756334
2307	18-19	6.3932475965680275
2307	20-21	6.329361813914861
2307	22-23	5.984112051384006
2307	24-25	6.22517145184818
2307	26-27	6.899988034765073
2307	28-29	6.104503242571937
2307	30-31	5.852510518573464
2307	32-33	5.999180742311346
2307	34-35	5.623378018331753
2307	36-37	5.467415718974994
2307	38-39	5.190325638871691
2307	40-41	4.517311409135587
2307	42-43	4.325443166251567
2307	44-45	4.300813271070073
2307	46-47	4.618079177552126
2307	48-49	4.332920484189284
2307	50-51	4.024950272834769
2307	52-53	4.76236318808537
2307	54-55	4.331240355109379
2307	56-57	4.603246329990846
2307	58-59	4.185674320321951
2307	60-61	4.236488632088008
2307	62-63	3.8535996694815537
2307	64-65	3.739698450342285
2307	66-67	3.436372342186921
2307	68-69	3.625695008871439
2307	70-71	3.190459854260485
2307	72-73	2.6325151798448054
2307	74-75	2.333326609376293
2307	76-77	2.576503168853449
2307	78-79	2.864299277785456
2307	80-81	2.633394179370349
2307	82-83	2.7569187208744097
2307	84-85	2.094543701234592
2307	86-87	2.099331118767523
2307	88-89	2.2663310777835655
2307	90-91	1.9706444206689184
2307	92-93	1.9036128767773448
2307	94-95	2.1178286025326223
2307	96-97	1.888565424298612
2307	98-99	1.6009102058759765
2307	100-101	1.3720665897547555
1101	1	7.994967017934304
1101	2	7.834482508881447
1101	3	7.223294050525666
1101	4	8.619430284169855
1101	5	8.543545274786034
1101	6	7.331401170412793
1101	7	7.021001576726924
1101	8	8.001626383791837
1101	9	7.358470777777487
1101	10-11	7.471228604495568
1101	12-13	7.214330092335924
1101	14-15	7.63646360951336
1101	16-17	6.7876073802254275
1101	18-19	5.959894229779156
1101	20-21	5.742332638855206
1101	22-23	5.19942854941203
1101	24-25	5.950103908031078
1101	26-27	6.4877740171585785
1101	28-29	5.884076253587736
1101	30-31	5.609261269639607
1101	32-33	5.68868139419429
1101	34-35	5.409642848386152
1101	36-37	5.303292421766368
1101	38-39	4.967955588954716
1101	40-41	4.4579944327166885
1101	42-43	4.230531496453835
1101	44-45	4.14586935008899
1101	46-47	4.563669275463958
1101	48-49	4.280604572340842
1101	50-51	3.7550965614486884
1101	52-53	4.704267314432826
1101	54-55	4.5297976196783765
1101	56-57	4.628360217924268
1101	58-59	4.133747352317613
1101	60-61	4.406003726149354
1101	62-63	3.956880365580485
1101	64-65	3.79780904757963
1101	66-67	3.458605306986751
1101	68-69	3.5438877315512087
1101	70-71	3.4684848788270095
1101	72-73	3.016369588214232
1101	74-75	2.408069983072185
1101	76-77	2.7509800858654465
1101	78-79	3.2283601128237684
1101	80-81	2.8688417625290796
1101	82-83	3.0636443338064403
1101	84-85	2.248073203303555
1101	86-87	2.3856315517564965
1101	88-89	2.428692243298517
1101	90-91	2.376517723970494
1101	92-93	2.088145977079378
1101	94-95	2.2979278686398104
1101	96-97	2.184286941511264
1101	98-99	1.9905269881495862
1101	100-101	1.6682572265095992
2201	1	7.0108539060867585
2201	2	7.806376926726173
2201	3	6.243330380223924
2201	4	8.30543264044756
2201	5	3.7636751314863446
2201	6	5.682516078923353
2201	7	6.544010094025097
2201	8	7.743912406010466
2201	9	6.376256967951317
2201	10-11	4.1010382834177115
2201	12-13	6.351887994909969
2201	14-15	6.926936011386996
2201	16-17	5.8971738687158
2201	18-19	1.9564583209827475
2201	20-21	5.00507724273503
2201	22-23	5.058848024640394
2201	24-25	5.291621482748891
2201	26-27	5.955648361689583
2201	28-29	4.858486869387903
2201	30-31	4.955161703936401
2201	32-33	5.157412171186948
2201	34-35	4.76085539492021
2201	36-37	4.813629117456781
2201	38-39	4.301142621164843
2201	40-41	3.9789639339436036
2201	42-43	3.738752608930625
2201	44-45	3.6053498079971114
2201	46-47	4.077935318826288
2201	48-49	3.824241740161586
2201	50-51	3.571426686482905
2201	52-53	4.322275327840114
2201	54-55	3.992174977365611
2201	56-57	4.096929173381476
2201	58-59	3.727776369252034
2201	60-61	3.7396352796194066
2201	62-63	3.518703746693866
2201	64-65	3.3784946799945423
2201	66-67	3.039749614104423
2201	68-69	3.233190590804554
2201	70-71	3.0539989034022206
2201	72-73	2.3655417179969014
2201	74-75	2.0636997993954864
2201	76-77	2.4309542941360647
2201	78-79	2.7401436655725497
2201	80-81	2.2478955621469723
2201	82-83	2.5807542735938824
2201	84-85	2.164995795803687
2201	86-87	2.173538853228923
2201	88-89	2.193237499854824
2201	90-91	2.0088491428892965
2201	92-93	1.8341715623980654
2201	94-95	1.89840397346671
2201	96-97	1.8373861384361163
2201	98-99	1.811840601592312
2201	100-101	1.4771803679549826
2304	1	1.8214150489315841
2304	2	4.987745278627894
2304	3	5.75452302340171
2304	4	4.324564861905916
2304	5	6.511764114744938
2304	6	6.33031227075848
2304	7	7.187635167442522
2304	8	6.36548294578413
2304	9	5.720424642976109
2304	10-11	6.640556471384809
2304	12-13	6.554095203583099
2304	14-15	6.839260631670765
2304	16-17	5.401665830013446
2304	18-19	5.248852430372633
2304	20-21	4.978447715121842
2304	22-23	4.545628914214124
2304	24-25	4.648738185037072
2304	26-27	5.6926673480130745
2304	28-29	4.893206554500715
2304	30-31	5.015632470273683
2304	32-33	5.047443938926968
2304	34-35	4.710112149159617
2304	36-37	4.7760614728736535
2304	38-39	4.438088712593867
2304	40-41	3.8457351434338847
2304	42-43	3.2595664496547307
2304	44-45	3.108971687345015
2304	46-47	4.053327622282929
2304	48-49	3.781322245484972
2304	50-51	3.4599598352310785
2304	52-53	3.950311810754119
2304	54-55	3.7086827559654694
2304	56-57	4.489457851720143
2304	58-59	3.705043524037052
2304	60-61	3.6902187665272272
2304	62-63	3.4790522299557836
2304	64-65	3.25545754949653
2304	66-67	3.080931758784441
2304	68-69	3.3209072325107627
2304	70-71	3.152517397060901
2304	72-73	2.857830973767502
2304	74-75	2.3930622371301595
2304	76-77	2.6065973517540897
2304	78-79	2.951872136385557
2304	80-81	2.7232723859182357
2304	82-83	2.917313077975951
2304	84-85	2.4293167681360437
2304	86-87	2.3227883260921907
2304	88-89	2.480595688969629
2304	90-91	2.348476241131994
2304	92-93	2.228679895459134
2304	94-95	2.438987033717318
2304	96-97	2.3969352646535924
2304	98-99	2.1465409551279784
2304	100-101	1.7320808506417045
1102	1	6.944043091807416
1102	2	7.409769185211648
1102	3	6.083941609800185
1102	4	6.015400168998504
1102	5	6.612641540780238
1102	6	5.774448803444326
1102	7	6.783796341572369
1102	8	6.221092487719794
1102	9	4.749285126931589
1102	10-11	5.853226998459331
1102	12-13	5.804647406852109
1102	14-15	6.087439410269251
1102	16-17	5.218966802826369
1102	18-19	5.225386219232359
1102	20-21	5.478524056520808
1102	22-23	5.17090675153926
1102	24-25	1.78955460607634
1102	26-27	5.280646904114008
1102	28-29	4.830684796767173
1102	30-31	4.7948649645473544
1102	32-33	4.829737473997561
1102	34-35	4.832101631816553
1102	36-37	4.758500458093973
1102	38-39	3.791024660464302
1102	40-41	3.874278854791214
1102	42-43	4.039267377477394
1102	44-45	-3.914462237775478
1102	46-47	1.099011832977073
1102	48-49	-0.934426976425577
1102	50-51	2.481801352979833
1102	52-53	-0.30990353663548476
1102	54-55	2.5259842795612215
1102	56-57	-0.7861393526801486
1102	58-59	2.6622718819316766
1102	60-61	3.711990849403815
1102	62-63	3.405786815392421
1102	64-65	3.052760271769653
1102	66-67	3.0183702577215037
1102	68-69	3.376493511119037
1102	70-71	3.14505444916394
1102	72-73	2.7057008864002974
1102	74-75	2.5017924131154174
1102	76-77	2.644386402220441
1102	78-79	-1.3824671438079612
1102	80-81	1.5422668709877456
1102	82-83	2.5656951316740866
1102	84-85	2.1644970864624256
1102	86-87	2.1699515843552746
1102	88-89	2.219646423460272
1102	90-91	2.043611509771246
1102	92-93	2.033815626466076
1102	94-95	2.319691879200267
1102	96-97	2.297295237468113
1102	98-99	2.2377603317838606
1102	100-101	1.886882836242794
2202	1	-20.565513222185757
2202	2	-7.712894661734346
2202	3	-0.6625795812979369
2202	4	1.4757770003891046
2202	5	0.9939880321736787
2202	6	1.5426165529607303
2202	7	3.868784494895557
2202	8	2.714521189466243
2202	9	3.1974193528901047
2202	10-11	2.1313723178009703
2202	12-13	1.3854727256019324
2202	14-15	2.5960908570624603
2202	16-17	1.5986018300443767
2202	18-19	1.651570874981914
2202	20-21	1.1644335508690382
2202	22-23	1.2724358263689233
2202	24-25	2.3207366933091826
2202	26-27	3.4745363956908726
2202	28-29	-0.9539417835387809
2202	30-31	1.0953527281782733
2202	32-33	1.5686553624397703
2202	34-35	1.6842212507183554
2202	36-37	3.02591189119903
2202	38-39	2.445473770503888
2202	40-41	1.892120508480069
2202	42-43	0.7951346762425828
2202	44-45	0.7230032813393272
2202	46-47	-1.3075786754587817
2202	48-49	0.8070384367349348
2202	50-51	1.7203310525580413
2202	52-53	1.6760388735447833
2202	54-55	-0.7355472468536135
2202	56-57	1.1110238403241972
2202	58-59	1.898959758548692
2202	60-61	2.0980372411588526
2202	62-63	-2.213344545504622
2202	64-65	0.04910819736427996
2202	66-67	0.6691068309972508
2202	68-69	1.5566666143501848
2202	70-71	-2.484500913467823
2202	72-73	-4.446522618621952
2202	74-75	-1.1077106453206014
2202	76-77	-0.1984114816845164
2202	78-79	1.5518475872280106
2202	80-81	1.4983107761576946
2202	82-83	-0.7338514284782782
2202	84-85	-0.3740437494533424
2202	86-87	0.530654678618486
2202	88-89	1.1424575723341732
2202	90-91	1.2732276927151815
2202	92-93	1.3105735172373763
2202	94-95	1.6867405381545417
2202	96-97	1.189504709114125
2202	98-99	1.1628241072027485
2202	100-101	0.8449764559327271
2305	1	5.737553127272346
2305	2	7.0828551752569275
2305	3	6.638800918419037
2305	4	7.632122726082063
2305	5	7.741839253919402
2305	6	6.011330614384054
2305	7	2.6969789918156444
2305	8	5.927523088422237
2305	9	6.2865389091204555
2305	10-11	4.824420743784881
2305	12-13	5.191624984825783
2305	14-15	5.539820594640869
2305	16-17	5.024118690334614
2305	18-19	4.425500526186678
2305	20-21	5.194039082878174
2305	22-23	5.123250359867878
2305	24-25	5.365696299786034
2305	26-27	6.056109116508409
2305	28-29	5.124271833799206
2305	30-31	5.368562411090583
2305	32-33	5.452721291488956
2305	34-35	5.272246784507008
2305	36-37	4.6138144684095685
2305	38-39	4.583394242725163
2305	40-41	4.050454831565332
2305	42-43	3.9656975000213066
2305	44-45	3.139507650867717
2305	46-47	3.901135398163163
2305	48-49	3.4136078371241716
2305	50-51	2.310632147110937
2305	52-53	3.908454142184528
2305	54-55	3.974692033578428
2305	56-57	4.558135646214673
2305	58-59	4.284947377389624
2305	60-61	4.456959358604756
2305	62-63	3.8668476146028254
2305	64-65	4.080481106580315
2305	66-67	3.684148722363485
2305	68-69	4.0159182683633645
2305	70-71	3.7045315963438448
2305	72-73	-0.9500537966211944
2305	74-75	2.043685821654968
2305	76-77	2.9818629341095537
2305	78-79	3.5929153153523394
2305	80-81	3.163536129273645
2305	82-83	3.254663382849863
2305	84-85	2.8452511605084325
2305	86-87	2.793029950929647
2305	88-89	2.935068389341403
2305	90-91	2.8204922290660264
2305	92-93	2.80498573452088
2305	94-95	2.901671843455663
2305	96-97	2.8200478698210425
2305	98-99	2.7273427460152995
2305	100-101	2.403036339565997
1103	1	-20.565513222185757
1103	2	-7.745800232321603
1103	3	-0.741158058243041
1103	4	2.917246883058521
1103	5	4.634771102723743
1103	6	4.440642680304851
1103	7	3.87866977133805
1103	8	4.045093956729378
1103	9	4.229282992038769
1103	10-11	4.8364724104253405
1103	12-13	4.460826190147461
1103	14-15	3.904005066642373
1103	16-17	2.726483611136185
1103	18-19	2.9456465586104237
1103	20-21	2.377372135705002
1103	22-23	2.5742164390284366
1103	24-25	2.9740672929628573
1103	26-27	4.0485584166188495
1103	28-29	3.5950873651194897
1103	30-31	3.5248700095024112
1103	32-33	2.9114038277372245
1103	34-35	2.37923526816488
1103	36-37	2.5607859688750274
1103	38-39	2.324813548791937
1103	40-41	2.550281563436478
1103	42-43	2.2844490165095905
1103	44-45	2.639025087410314
1103	46-47	1.8414254182658851
1103	48-49	-1.2118930238462475
1103	50-51	-1.894015616813018
1103	52-53	1.6142793506100865
1103	54-55	2.261922960483382
1103	56-57	3.20483920022469
1103	58-59	2.6935912997032005
1103	60-61	3.0945893761371472
1103	62-63	2.8012329174498305
1103	64-65	2.732420572834016
1103	66-67	2.4389651112954915
1103	68-69	2.8591469668656018
1103	70-71	2.759983672066131
1103	72-73	2.24108436811186
1103	74-75	1.867789273043897
1103	76-77	2.15690591250398
1103	78-79	2.732373792380667
1103	80-81	2.34290104852505
1103	82-83	2.475263994516439
1103	84-85	1.9506071147719979
1103	86-87	1.7880758554498115
1103	88-89	1.8990925998576955
1103	90-91	-2.5040216660819716
1103	92-93	-3.2460711733835943
1103	94-95	-3.400549350980892
1103	96-97	0.1150813250196876
1103	98-99	-1.2363532349834827
1103	100-101	0.5672802789461784
2203	1	2.51391026754132
2203	2	3.279985926030072
2203	3	-5.668154786434979
2203	4	-3.040556409575668
2203	5	-0.004567527398556592
2203	6	0.13848640576275884
2203	7	2.2684086749402432
2203	8	-7.473187518099312
2203	9	-6.966214840100445
2203	10-11	-12.245108642631521
2203	12-13	-10.082923356528958
2203	14-15	-1.535848688923501
2203	16-17	-1.2792086558208133
2203	18-19	-0.639157833817805
2203	20-21	-1.3024143896728582
2203	22-23	-3.0270941338860737
2203	24-25	-0.9138359659230737
2203	26-27	-1.5194650823052918
2203	28-29	0.5242744115443614
2203	30-31	-2.0256160432885544
2203	32-33	-0.05488281354588764
2203	34-35	-0.019832959741766132
2203	36-37	-0.3410302455470493
2203	38-39	0.48664897428583487
2203	40-41	-0.807036472800462
2203	42-43	0.20043153386904677
2203	44-45	0.04673551454196101
2203	46-47	0.41934218244796284
2203	48-49	0.4934396927529363
2203	50-51	0.27922378145243343
2203	52-53	1.0905011320469349
2203	54-55	0.17059501162795598
2203	56-57	0.47021891176060393
2203	58-59	0.6010165665015208
2203	60-61	0.24160419274265976
2203	62-63	0.7144246314269935
2203	64-65	0.2888902827694402
2203	66-67	-0.5137083567448393
2203	68-69	0.3841437534597425
2203	70-71	0.5472779552180107
2203	72-73	0.028800911204765356
2203	74-75	-0.5017145933620029
2203	76-77	-0.2944726236233066
2203	78-79	0.601272742091485
2203	80-81	0.33704500705228924
2203	82-83	-0.1317306459250709
2203	84-85	-0.23837745839650637
2203	86-87	-0.13140721908553488
2203	88-89	0.19994659939407455
2203	90-91	-0.2680579984199838
2203	92-93	-0.11081069997119997
2203	94-95	0.3564674343441574
2203	96-97	0.1544381394653449
2203	98-99	-0.11374735079848364
2203	100-101	-0.5897834850451957
2310	1	-20.565513222185757
2310	2	-20.41013980227413
2310	3	-21.2788570250121
2310	4	-23.2690139379412
2310	5	-23.4343436696862
2310	6	-24.488008534439633
2310	7	-24.058638243183033
2310	8	-23.688918888844483
2310	9	-24.601909412317557
2310	10-11	-24.329821920767063
2310	12-13	-24.598026085753123
2310	14-15	-24.92376650554417
2310	16-17	-25.956914880905142
2310	18-19	-20.119288717176047
2310	20-21	-20.198068509840787
2310	22-23	-26.51367800386461
2310	24-25	-26.119831059452668
2310	26-27	-19.085004564823443
2310	28-29	-19.54595947728562
2310	30-31	-20.073166322894203
2310	32-33	-18.2697150670059
2310	34-35	-10.628570431522387
2310	36-37	-15.232626112507155
2310	38-39	-19.961906556531524
2310	40-41	-12.080971561908527
2310	42-43	-5.435195680960582
2310	44-45	-14.366163154791103
2310	46-47	-19.431310286895805
2310	48-49	-19.89674390256106
2310	50-51	-11.815485701315712
2310	52-53	-15.348839151910491
2310	54-55	-19.193628736995997
2310	56-57	-18.70041721704929
2310	58-59	-11.266404052288348
2310	60-61	-8.218334599415169
2310	62-63	-5.355399490166455
2310	64-65	-15.799413253834304
2310	66-67	-11.388685639789987
2310	68-69	-6.197475845714038
2310	70-71	-4.069659289091348
2310	72-73	-4.762214643845574
2310	74-75	-4.563719399991442
2310	76-77	-3.566030553366769
2310	78-79	-4.0729827001620755
2310	80-81	-3.3183378865850237
2310	82-83	-13.006832573443504
2310	84-85	-10.985282447176935
2310	86-87	-6.784198303114721
2310	88-89	-4.978606882880982
2310	90-91	-2.5526724269444685
2310	92-93	-2.0172993088142697
2310	94-95	-2.4973762234778043
2310	96-97	-1.4670908685284374
2310	98-99	-1.6391528019115604
2310	100-101	-1.6228756131021314
2204	1	-20.565513222185757
2204	2	-20.41013980227413
2204	3	-21.2788570250121
2204	4	-23.2690139379412
2204	5	-23.4343436696862
2204	6	-24.488008534439633
2204	7	-24.058638243183033
2204	8	-23.688918888844483
2204	9	-24.601909412317557
2204	10-11	-18.305127419748732
2204	12-13	-18.803601442168603
2204	14-15	-18.79571151572747
2204	16-17	-20.089170481719805
2204	18-19	-26.378424929800634
2204	20-21	-14.085917866234404
2204	22-23	-6.563576170870718
2204	24-25	-15.861175254972016
2204	26-27	-25.23948961465733
2204	28-29	-13.01695132231834
2204	30-31	-15.683392503784786
2204	32-33	-19.24549967291768
2204	34-35	-19.545357449624355
2204	36-37	-25.76789317103003
2204	38-39	-13.469273476188857
2204	40-41	-7.356121943190203
2204	42-43	-5.052499326493283
2204	44-45	-14.141588189780743
2204	46-47	-20.345772296136502
2204	48-49	-18.700465035782976
2204	50-51	-10.707979057317925
2204	52-53	-3.2151884546941005
2204	54-55	-25.291763160711817
2204	56-57	-19.039012572340518
2204	58-59	-11.827560516484407
2204	60-61	-15.72127856316231
2204	62-63	-12.431091586281394
2204	64-65	-5.939890606728177
2204	66-67	-3.996734824891817
2204	68-69	-7.275496171194458
2204	70-71	-7.372253018125537
2204	72-73	-3.9077963007383936
2204	74-75	-3.7580191205427624
2204	76-77	-3.7284448098818146
2204	78-79	-17.673229709582344
2204	80-81	-7.6754342578640085
2204	82-83	-4.303379236532702
2204	84-85	-3.057801500850964
2204	86-87	-2.5885121392336004
2204	88-89	-3.691269430511909
2204	90-91	-2.8537191103326016
2204	92-93	-2.488317178123477
2204	94-95	-1.9107990508280714
2204	96-97	-1.9717882829645177
2204	98-99	-2.424222596418666
2204	100-101	-2.666865260905187
2311	1	8.321348091682857
2311	2	8.507839452431977
2311	3	7.562479893927584
2311	4	9.035634544578972
2311	5	8.850615992242346
2311	6	7.98750049360493
2311	7	8.253405552437407
2311	8	8.322414188374111
2311	9	7.63070679974545
2311	10-11	7.8076636727788475
2311	12-13	7.3906888585419175
2311	14-15	8.077722161378613
2311	16-17	6.953332910105228
2311	18-19	6.19135032951554
2311	20-21	6.20857577771238
2311	22-23	5.814308934283684
2311	24-25	5.743691798787825
2311	26-27	6.543116993102949
2311	28-29	5.494623622931272
2311	30-31	5.052999754325665
2311	32-33	5.3157396889967075
2311	34-35	5.066864279885618
2311	36-37	5.1182478201340125
2311	38-39	4.473304383213538
2311	40-41	3.6305520378734784
2311	42-43	3.5729077316325046
2311	44-45	3.254811849575379
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2311	50-51	3.2596413857240627
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2311	54-55	3.3606571235755425
2311	56-57	3.556647652075455
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2311	60-61	3.052025123325084
2311	62-63	2.7366847750845054
2311	64-65	2.6029335316426376
2311	66-67	2.106087206791816
2311	68-69	2.283799052943756
2311	70-71	2.0730414310178205
2311	72-73	1.5548008572486722
2311	74-75	1.1586313759468752
2311	76-77	1.278153502577645
2311	78-79	1.6056005098182702
2311	80-81	1.4639621713696869
2311	82-83	1.545971455876888
2311	84-85	0.9446661790076973
2311	86-87	0.9022467023975089
2311	88-89	0.6663935805211025
2311	90-91	0.5779594792315308
2311	92-93	0.47092849876665355
2311	94-95	0.8064321832250734
2311	96-97	0.6729274824886247
2311	98-99	0.5790362158713975
2311	100-101	0.2968586910912947
2205	1	8.392992602402671
2205	2	8.54143428071422
2205	3	7.60349591616437
2205	4	9.294575163820621
2205	5	9.153795350242554
2205	6	7.9509054927096905
2205	7	8.314520512954445
2205	8	8.693482189426433
2205	9	7.651676801218596
2205	10-11	7.878322875613026
2205	12-13	7.579118421804399
2205	14-15	8.075944671889157
2205	16-17	6.9600004940828235
2205	18-19	6.379397348046677
2205	20-21	6.2939183431351005
2205	22-23	5.957295328185072
2205	24-25	5.988525540142984
2205	26-27	6.682143195586249
2205	28-29	5.892824051959828
2205	30-31	5.703710727929934
2205	32-33	5.692599185380239
2205	34-35	5.4695745471709465
2205	36-37	5.501722694956303
2205	38-39	4.825999326333175
2205	40-41	4.540096589022539
2205	42-43	4.279767311049305
2205	44-45	4.268063055009225
2205	46-47	4.513512768501521
2205	48-49	4.016159781349987
2205	50-51	3.9641503566624614
2205	52-53	4.695547247265324
2205	54-55	4.272162900711116
2205	56-57	4.459051061565013
2205	58-59	4.046884632423918
2205	60-61	4.107113099551757
2205	62-63	3.6738979289496356
2205	64-65	3.4950952472405454
2205	66-67	3.1897717470470575
2205	68-69	3.0707254933607757
2205	70-71	2.9906513726833523
2205	72-73	2.396253310762905
2205	74-75	2.058689872780203
2205	76-77	2.3497856646440525
2205	78-79	2.7219396839706604
2205	80-81	2.4109165449862573
2205	82-83	2.424060630334413
2205	84-85	1.697161938486591
2205	86-87	1.586409826712785
2205	88-89	1.6400808007716314
2205	90-91	1.4856439006142246
2205	92-93	1.23893684377488
2205	94-95	1.539894201763051
2205	96-97	1.2312298025634334
2205	98-99	1.0177459793723536
2205	100-101	0.8543804893540745
2206	1	8.103255872127832
2206	2	8.26079742055035
2206	3	7.319349341401619
2206	4	8.805589393092607
2206	5	8.749947253739442
2206	6	7.555551138370067
2206	7	8.15373979958823
2206	8	8.52276929485513
2206	9	7.417505192984503
2206	10-11	7.770700401101468
2206	12-13	7.507670179152765
2206	14-15	8.191144305532998
2206	16-17	7.009331300232137
2206	18-19	6.421910144605594
2206	20-21	6.31019243354017
2206	22-23	5.816616664510281
2206	24-25	6.265455232062063
2206	26-27	6.865960272008678
2206	28-29	5.8382959671697705
2206	30-31	5.830097394807741
2206	32-33	5.8961141487109
2206	34-35	5.368390483758983
2206	36-37	5.545401398793569
2206	38-39	5.004780599469235
2206	40-41	4.537308328355749
2206	42-43	4.1985995405281
2206	44-45	4.172973559530856
2206	46-47	4.530746028583792
2206	48-49	4.321458161646582
2206	50-51	4.269958486350234
2206	52-53	4.829081663067672
2206	54-55	4.345715897137794
2206	56-57	4.596807371431048
2206	58-59	4.194046428632156
2206	60-61	4.311816921502324
2206	62-63	3.7689140161891714
2206	64-65	3.5932920260847467
2206	66-67	3.277384337775427
2206	68-69	3.546461152882948
2206	70-71	3.3837119069463704
2206	72-73	2.7195555953303376
2206	74-75	2.2961223220434803
2206	76-77	2.550308684825417
2206	78-79	3.1062508518268572
2206	80-81	2.6527430755587105
2206	82-83	2.7496030616674645
2206	84-85	2.119499983018436
2206	86-87	2.0983800566879793
2206	88-89	2.228422265005932
2206	90-91	2.072307729942242
2206	92-93	1.8405952380753057
2206	94-95	2.063866090534834
2206	96-97	1.8063574327537282
2206	98-99	1.6755484976231543
2206	100-101	1.4167834507925328
2309	1	8.028385226035503
2309	2	8.123482070416674
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2104	10-11	-12.67319996724354
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2104	16-17	-10.781683443157174
2104	18-19	-4.0123754304618835
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2104	22-23	-2.463706343180686
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2104	28-29	-1.9838300592890832
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2104	32-33	-1.681794746955827
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2104	36-37	-4.048830257748335
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2104	96-97	-3.343348725935158
2104	98-99	-2.4354653014015746
2104	100-101	-1.604984618293397
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2110	2	-20.41013980227413
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2110	4	-23.2690139379412
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2110	30-31	-19.845577776654427
2110	32-33	-19.67168754210581
2110	34-35	-19.7612317682089
2110	36-37	-25.76789317103003
2110	38-39	-15.230668063369738
2110	40-41	-18.160276500502725
2110	42-43	-14.801229523284174
2110	44-45	-8.708043732490406
2110	46-47	-4.012701359780383
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2110	64-65	-3.13705211773679
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2110	82-83	-2.3894117619014033
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2110	86-87	-2.3325926922658553
2110	88-89	-2.421446260247343
2110	90-91	-2.152543105907071
2110	92-93	-1.8092001801223283
2110	94-95	-1.9290551147913284
2110	96-97	-1.9255864123615716
2110	98-99	-2.2838686732864097
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2109	1	7.727799847723059
2109	2	7.8114914033996286
2109	3	6.996218962829843
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2109	5	8.179891386038214
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2109	66-67	2.4804742391732084
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2109	72-73	1.7887256433948089
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2108	2	7.167172105781631
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2108	82-83	1.9219018120187847
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2211	1	6.421679115491045
2211	2	6.155290646550906
2211	3	5.559766429715598
2211	4	7.568384436042539
2211	5	7.174410089084262
2211	6	6.943219887433624
2211	7	6.886121752362129
2211	8	6.52480201548785
2211	9	5.563587859093513
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2211	14-15	6.377826099422979
2211	16-17	5.288379695328292
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2211	32-33	5.015425315213712
2211	34-35	4.493564651140748
2211	36-37	4.819421674721166
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2211	42-43	3.41533827783762
2211	44-45	3.6170749439762524
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2211	54-55	3.2762845060662187
2211	56-57	3.7267428483752525
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2211	60-61	0.5695878514339583
2211	62-63	1.1274063736563562
2211	64-65	2.675337582422838
2211	66-67	2.753508884179066
2211	68-69	2.8539604257666795
2211	70-71	2.7831330983594285
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2211	74-75	1.8700718493578137
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2211	78-79	2.557736372398036
2211	80-81	2.470738462076092
2211	82-83	2.5460902287663956
2211	84-85	2.0341842062379243
2211	86-87	2.020004133971426
2211	88-89	2.096210092879282
2211	90-91	2.0167219947215287
2211	92-93	1.809697804335027
2211	94-95	1.9464009580634603
2211	96-97	1.7660159004333096
2211	98-99	1.6972169630512788
2211	100-101	1.5410742620459672
2210	1	7.434099431138968
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2210	3	6.87969688073354
2210	4	8.577855009767003
2210	5	8.606715077892886
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2210	14-15	4.949312900524262
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2210	32-33	5.439770076043281
2210	34-35	5.2239605947351215
2210	36-37	5.199376034909289
2210	38-39	4.5241568658358915
2210	40-41	4.441632668878029
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2210	46-47	4.693652472356142
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2210	54-55	4.519663566208777
2210	56-57	4.711979658373995
2210	58-59	4.288005501405067
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2210	62-63	3.8117432069430635
2210	64-65	3.7731488155914583
2210	66-67	3.4643489470443534
2210	68-69	3.6053653070077587
2210	70-71	3.720460285510338
2210	72-73	3.2835776919433073
2210	74-75	2.8068978024047055
2210	76-77	3.0064679976715745
2210	78-79	3.6549655717830767
2210	80-81	3.362106600241674
2210	82-83	3.222503847488394
2210	84-85	2.6825827757140353
2210	86-87	2.6561066214411113
2210	88-89	2.913611042271924
2210	90-91	2.773210260038404
2210	92-93	2.5494087905390437
2210	94-95	2.8879425984062443
2210	96-97	2.797884960662863
2210	98-99	2.6675963814647083
2210	100-101	2.3615754156235234
2209	1	4.971765495994887
2209	2	2.562078830624177
2209	3	4.448756361730371
2209	4	1.9594393505973713
2209	5	4.824475299172825
2209	6	5.112585857600681
2209	7	6.20379876418226
2209	8	6.942816477481912
2209	9	6.189407295267404
2209	10-11	6.13083191047728
2209	12-13	5.58281123172976
2209	14-15	4.880084121151786
2209	16-17	4.386217306715995
2209	18-19	3.7103462292638447
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2209	22-23	3.824091733827604
2209	24-25	4.166704668677578
2209	26-27	1.5725662065081956
2209	28-29	0.13344672639359345
2209	30-31	2.936772550800274
2209	32-33	4.196109355493711
2209	34-35	4.1251456249715055
2209	36-37	3.8238499482360275
2209	38-39	-1.2566557346680725
2209	40-41	-0.28588356003339754
2209	42-43	-4.418829409609742
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2209	48-49	1.7455995973116565
2209	50-51	2.8205190323430074
2209	52-53	3.6518736476420735
2209	54-55	3.080636374111805
2209	56-57	3.5153102441714026
2209	58-59	2.7376263172555646
2209	60-61	2.766336619046246
2209	62-63	2.7483539160267227
2209	64-65	2.1633094809595157
2209	66-67	2.1644116279014405
2209	68-69	-0.6798133230075933
2209	70-71	1.6326939054232632
2209	72-73	1.9821450331146124
2209	74-75	1.6545018112100536
2209	76-77	2.084271366471505
2209	78-79	2.3414852501436876
2209	80-81	2.056091932387236
2209	82-83	2.047017368042905
2209	84-85	1.3294168794057235
2209	86-87	1.3901910934474841
2209	88-89	1.7768864916049907
2209	90-91	1.6581256037599843
2209	92-93	1.464806584162865
2209	94-95	1.580600804797772
2209	96-97	1.4566355011121637
2209	98-99	1.2364479328576259
2209	100-101	1.1329959144994142
2208	1	4.648961746437866
2208	2	6.259920161468724
2208	3	4.604701753400953
2208	4	2.9245434459662043
2208	5	3.974525386233477
2208	6	4.887671287063643
2208	7	3.455934340835931
2208	8	4.085868449044234
2208	9	3.943621197081413
2208	10-11	4.362759309186917
2208	12-13	4.961170678993774
2208	14-15	5.650002843221696
2208	16-17	3.9115832365122323
2208	18-19	1.9207662569236739
2208	20-21	4.209283678173502
2208	22-23	3.347359508337316
2208	24-25	3.9408299362243646
2208	26-27	4.99959837864904
2208	28-29	4.282508505552514
2208	30-31	4.44014495161446
2208	32-33	3.478694410743003
2208	34-35	3.7347569672919327
2208	36-37	3.789630186939572
2208	38-39	3.775776756545902
2208	40-41	3.190310053642481
2208	42-43	3.245425444613243
2208	44-45	-1.2215338885544575
2208	46-47	2.6502165652851346
2208	48-49	2.9591886070719156
2208	50-51	3.2064555068536187
2208	52-53	1.8356598967928939
2208	54-55	0.9525542287736108
2208	56-57	-1.0717120256999877
2208	58-59	1.9449406912824472
2208	60-61	3.1178281643759966
2208	62-63	2.3912063069642286
2208	64-65	2.881414642037715
2208	66-67	2.922830795829345
2208	68-69	2.000718866404654
2208	70-71	0.057359256051878305
2208	72-73	0.04718720731523263
2208	74-75	-2.0157289474905724
2208	76-77	0.5885354832824632
2208	78-79	1.9734265939097995
2208	80-81	2.185118887285853
2208	82-83	2.3246378552499145
2208	84-85	1.6044300248603385
2208	86-87	2.079031466049159
2208	88-89	2.3020067837091496
2208	90-91	2.060607440004084
2208	92-93	1.8897207708910102
2208	94-95	1.9076187760095458
2208	96-97	1.8500959093057148
2208	98-99	2.2763538229168496
2208	100-101	2.093652304469721
1307	1	7.332923157099856
1307	2	7.194938378761588
1307	3	6.904187014580497
1307	4	8.5380438729898
1307	5	4.952688319985295
1307	6	5.913798953725621
1307	7	3.8207190943582106
1307	8	5.702273192635936
1307	9	5.985380789948973
1307	10-11	6.8365817515898435
1307	12-13	5.9099354692605
1307	14-15	5.6060713943267295
1307	16-17	4.400853014662207
1307	18-19	5.231458874531601
1307	20-21	5.2538637092247065
1307	22-23	4.878016157661715
1307	24-25	5.4856774759081155
1307	26-27	6.2729906607694375
1307	28-29	5.632664842818514
1307	30-31	5.060272351137886
1307	32-33	5.6997685257675315
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1212	58-59	-2.5945768097634314
1212	60-61	-13.870614739333389
1212	62-63	-11.677261392798705
1212	64-65	-15.90460356497665
1212	66-67	-12.327540578455974
1212	68-69	-7.846523411520323
1212	70-71	-14.913037132178493
1212	72-73	-11.594677028844297
1212	74-75	-7.976110388363537
1212	76-77	-18.701077192773873
1212	78-79	-9.688499567016152
1212	80-81	-7.474140978841607
1212	82-83	-5.162831247734328
1212	84-85	-4.128834469282737
1212	86-87	-3.2369731881134385
1212	88-89	-3.1786726891677155
1212	90-91	-3.460513916849912
1212	92-93	-3.4030536852517876
1212	94-95	-3.360804778933975
1212	96-97	-12.922395462190588
1212	98-99	-10.008908823506243
1212	100-101	-6.35729805112922
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	750.0
3	1685.0
4	837.0
5	4885.0
6	5968.0
7	5610.0
8	10624.0
9	20109.0
10	27971.0
11	36722.0
12	50378.0
13	63893.0
14	73104.0
15	78766.0
16	83810.0
17	91112.0
18	102245.0
19	112083.0
20	121205.0
21	134085.0
22	145908.0
23	157768.0
24	164601.0
25	169933.0
26	178079.0
27	185275.0
28	188442.0
29	190698.0
30	196401.0
31	207858.0
32	224422.0
33	255144.0
34	286480.0
35	313171.0
36	324775.0
37	298360.0
38	106896.0
39	329.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.185227562613484	26.26416017264598	20.898768454019628	24.651843810720912
2	27.075517697508218	29.591972158065882	21.235259773626293	22.09725037079961
3	18.975821787557727	26.212252536620916	29.14583060829552	25.66609506752584
4	22.924926148118917	27.37087303351314	27.178814081255183	22.52538673711276
5	22.708545669811926	27.67748710744089	26.71602991316554	22.897937309581646
6	21.986190680012292	29.158183890132726	26.052413041251445	22.803212388603537
7	22.72552133703048	28.469286235283857	27.129797273529775	21.67539515415589
8	22.387829608750444	28.13018884260551	27.253148178433655	22.228833370210392
9	21.435463619856023	26.410432345066777	29.31453626571006	22.83956776936714
10-11	22.81178313269712	26.795561284718612	27.411480965548783	22.98117461703548
12-13	22.72244462642249	27.256857054239887	27.03323975170248	22.987458567635144
14-15	23.024439038966133	27.383839279878234	26.633661863987374	22.958059817168255
16-17	23.204171290556392	27.235002336544454	26.627723147222536	22.933103225676614
18-19	23.09867389359046	27.290666498072202	26.643815563763614	22.966844044573726
20-21	23.209595585830492	27.29712485597937	26.507598239315016	22.985681318875123
22-23	23.055031716098064	27.270248853680425	26.736757223725956	22.93796220649556
24-25	22.857732011474766	27.44491358941652	26.92516509776694	22.772189301341772
26-27	22.840511651968242	27.429069110663466	26.79230397574073	22.938115261627562
28-29	23.107929544523582	27.315426560428474	26.741841638147086	22.834802256900858
30-31	22.98193343659952	27.35739794898786	26.73822154903485	22.922447065377767
32-33	22.96031260456108	27.38211760304658	26.676498430704925	22.981071361687412
34-35	23.08282496511617	27.278105058298728	26.73054268592451	22.908527290660587
36-37	22.93929302134427	27.45583058600695	26.793468064006003	22.811408328642777
38-39	23.067835000369787	27.340970603874876	26.8393015191513	22.751892876604042
40-41	23.144612379420533	27.283813563969854	26.688648904630142	22.882925151979467
42-43	22.95222165189033	27.4547120265875	26.788588075996067	22.804478245526102
44-45	22.967699275888744	27.298254117159416	26.83915869972493	22.894887907226913
46-47	23.10605191892588	27.355739703303804	26.652652398038732	22.885555979731578
48-49	22.953743383945206	27.406999795564797	26.866271425275094	22.7729853952149
50-51	23.1501579941709	27.321351394248545	26.59934199505727	22.929148616523285
52-53	23.016572543646518	27.302778699682488	26.811982759691684	22.868665996979306
54-55	22.94486338478565	27.37037620854888	26.866508741896617	22.81825166476885
56-57	22.98365117359584	27.365354906163592	26.818281276093607	22.83271264414696
58-59	23.119813226697854	27.30164679708848	26.717045921698546	22.861494054515116
60-61	23.058188411181554	27.290844152715373	26.717748279258103	22.933219156844974
62-63	23.165503407031125	27.31914818560241	26.649925483443038	22.86542292392343
64-65	23.236546529015882	27.221135320960187	26.662849364250413	22.87946878577351
66-67	23.20461776291196	27.36278093105079	26.680139494921303	22.752461811115953
68-69	23.2641932389621	27.295055759084885	26.663213643687953	22.77753735826506
70-71	23.39695927648894	27.263494212062355	26.523288323752443	22.81625818769626
72-73	23.254479513451376	27.376797269456603	26.65704469408431	22.711678523007713
74-75	23.4042311177207	27.27283264846979	26.482520488491218	22.840415745318293
76-77	23.509390843933957	27.311299048897876	26.402071463490174	22.777238643677993
78-79	23.496257307361986	27.319286980967394	26.51648437795136	22.667971333719258
80-81	23.702653897557266	27.252728384511144	26.29401410552597	22.75060361240562
82-83	23.659512163076656	27.188078007444176	26.42179906642179	22.730610763057378
84-85	23.72268286869769	27.22224490231276	26.315331249099188	22.739740979890364
86-87	23.81633636821552	27.294278858913184	26.23829151117635	22.65109326169495
88-89	23.926711924778047	27.198773304602426	26.24725692826652	22.627257842353004
90-91	24.02731815650751	27.21939713021583	26.069247891777575	22.684036821499088
92-93	24.15321661427133	27.168742697199594	26.02635409190426	22.651686596624824
94-95	24.314101374553076	27.081797110656623	25.952757014877864	22.651344499912433
96-97	24.306281711683912	27.269835073446902	25.92492784782367	22.498955367045514
98-99	24.45115440120909	27.220848839610507	25.810148049189678	22.517848709990716
100-101	24.90782974531605	27.046604834058837	25.4603794536935	22.58518596693162
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	288.0
1	302.5
2	352.0
3	370.5
4	364.0
5	420.0
6	492.0
7	585.5
8	705.5
9	908.5
10	1266.5
11	1738.5
12	2295.0
13	3042.0
14	3969.5
15	5062.5
16	6613.0
17	8598.0
18	10852.5
19	13786.0
20	17574.5
21	22311.0
22	27620.5
23	33674.0
24	40753.5
25	48042.0
26	56233.0
27	65055.0
28	74074.5
29	83585.5
30	92457.0
31	100896.0
32	109431.5
33	117523.0
34	125519.5
35	132482.0
36	138104.5
37	142940.5
38	146108.0
39	146673.0
40	145032.5
41	141613.0
42	136229.5
43	129736.0
44	122632.5
45	115559.5
46	108617.0
47	101871.5
48	95774.5
49	89778.0
50	84457.0
51	79908.0
52	75897.0
53	73613.0
54	72413.5
55	71848.5
56	72263.0
57	73407.5
58	74545.5
59	75066.0
60	74698.5
61	73266.0
62	71682.5
63	69592.0
64	66321.5
65	63001.0
66	59551.0
67	55150.5
68	50561.0
69	46118.0
70	41509.0
71	36463.5
72	31789.0
73	27545.5
74	23469.5
75	19776.0
76	16428.0
77	13645.5
78	11200.0
79	9111.5
80	7331.5
81	5952.0
82	4844.5
83	3856.5
84	3081.0
85	2494.0
86	1990.0
87	1596.0
88	1282.0
89	1020.0
90	807.5
91	612.5
92	447.0
93	328.5
94	244.5
95	180.5
96	117.5
97	63.0
98	36.5
99	20.5
100	6.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	13.630647855523634
2	10.957968410404161
3	9.655651848699955
4	11.582981666883821
5	9.092127014606152
6	5.34185268663933
7	9.676126346263144
8	10.008306672478597
9	5.433490131335461
10-11	6.312291927377434
12-13	5.342382945825692
14-15	7.286453804036117
16-17	2.6698333600987967
18-19	3.1200342309358837
20-21	3.05145765003846
22-23	3.106983361981758
24-25	3.4890188733312524
26-27	4.33867805735543
28-29	1.723710290620992
30-31	2.2556901139343024
32-33	3.0375194085683828
34-35	2.0019123959880374
36-37	3.1015184458774185
38-39	1.3785223819156078
40-41	1.1450676589078566
42-43	0.553882774194861
44-45	1.3127594211907154
46-47	1.3816065424893438
48-49	1.2776973851945574
50-51	1.547058230250226
52-53	2.4414864398657943
54-55	1.8281388854860918
56-57	1.827327264282477
58-59	1.5486165429611665
60-61	1.403812498620244
62-63	0.6084778271580142
64-65	1.2325820678896249
66-67	0.5616310512853699
68-69	0.7999013934345689
70-71	0.9936732503935822
72-73	0.0836510920525619
74-75	0.10527268091685925
76-77	0.45296038292937685
78-79	0.8857492735449147
80-81	0.29294114642468955
82-83	0.5027939248313235
84-85	0.06533009608296457
86-87	0.09971037026808606
88-89	0.081183763593573
90-91	0.08898614876432295
92-93	0.16997512326902842
94-95	0.45521127906740183
96-97	0.36989365814341757
98-99	0.06561145810021769
100-101	0.22877978487493025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4620382.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.79207006271724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.01835910860174	97.82228670552281
2	0.8357876729927499	1.651383886957103
3	0.0929457396360134	0.2754690606645633
4	0.02537078964147161	0.10025731311226899
5	0.01777492770084733	0.08780109513909215
6	0.0063536826832617695	0.0376616078880642
7	0.0018169085903996611	0.01256473125322213
8	0.0015911701534973882	0.01257559946288148
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.9478908886754387E-4	0.0	0.0
2	2.164323209639376E-4	0.0	0.0
3	3.2464848144590644E-4	2.1643232096393763E-5	2.1643232096393763E-5
4	3.8957817773508773E-4	2.1643232096393763E-5	4.3286464192787526E-5
5	4.5450787402426897E-4	2.1643232096393763E-5	4.3286464192787526E-5
6	6.709401949882066E-4	2.1643232096393763E-5	4.3286464192787526E-5
7	8.007995875665691E-4	4.3286464192787526E-5	4.3286464192787526E-5
8	0.001103804836916082	4.3286464192787526E-5	4.3286464192787526E-5
9	0.0012336642294944445	4.3286464192787526E-5	4.3286464192787526E-5
10-11	0.0013959884702173977	4.3286464192787526E-5	6.492969628918129E-5
12-13	0.0015691343269885476	4.3286464192787526E-5	7.575131233737817E-5
14-15	0.0018721395763380603	4.3286464192787526E-5	9.739454443377193E-5
16-17	0.0021426799775429825	4.3286464192787526E-5	1.082161604819688E-4
18-19	0.002380755530603314	4.3286464192787526E-5	1.082161604819688E-4
20-21	0.0027811553243865987	4.3286464192787526E-5	1.082161604819688E-4
22-23	0.0029867660293023393	4.3286464192787526E-5	1.082161604819688E-4
24-25	0.00319237673421808	4.3286464192787526E-5	1.082161604819688E-4
26-27	0.003517025215663986	4.3286464192787526E-5	1.082161604819688E-4
28-29	0.004079749250170224	4.3286464192787526E-5	1.082161604819688E-4
30-31	0.00506451631055614	4.3286464192787526E-5	1.082161604819688E-4
32-33	0.006687758717785672	8.657292838557505E-5	1.082161604819688E-4
34-35	0.00915508717677456	1.082161604819688E-4	1.082161604819688E-4
36-37	0.012466501687522807	1.082161604819688E-4	1.4068100862655946E-4
38-39	0.017714985470898295	1.082161604819688E-4	1.5150262467475634E-4
40-41	0.02486807367875643	1.082161604819688E-4	1.5150262467475634E-4
42-43	0.035332576397362814	1.2985939257836258E-4	1.5150262467475634E-4
44-45	0.049108493626717445	1.6232424072295322E-4	1.9478908886754387E-4
46-47	0.06786235423824263	1.731458567711501E-4	1.9478908886754387E-4
48-49	0.09212441741830005	1.731458567711501E-4	2.164323209639376E-4
50-51	0.1224898720495405	1.731458567711501E-4	2.164323209639376E-4
52-53	0.15946733408622923	1.8396747281934698E-4	2.2725393701213448E-4
54-55	0.20457182977511384	1.9478908886754387E-4	2.3807555306033136E-4
56-57	0.25501138217575947	1.9478908886754387E-4	2.4889716910852827E-4
58-59	0.3190320627168922	1.9478908886754387E-4	2.5971878515672515E-4
60-61	0.3940258619308966	1.9478908886754387E-4	2.5971878515672515E-4
62-63	0.47788256468837426	1.9478908886754387E-4	2.5971878515672515E-4
64-65	0.5748442444802182	1.9478908886754387E-4	2.7054040120492204E-4
66-67	0.6813073031623793	1.9478908886754387E-4	2.813620172531189E-4
68-69	0.800788765950521	1.9478908886754387E-4	2.813620172531189E-4
70-71	0.9368164796763558	1.9478908886754387E-4	2.921836333013158E-4
72-73	1.0764910780104329	1.9478908886754387E-4	3.030052493495127E-4
74-75	1.228415745711069	1.9478908886754387E-4	3.2464848144590644E-4
76-77	1.3901556191674196	1.9478908886754387E-4	3.462917135423002E-4
78-79	1.5684525651775112	1.9478908886754387E-4	3.571133295904971E-4
80-81	1.7736520486834206	2.164323209639376E-4	3.7875656168689085E-4
82-83	1.9895541104609966	2.3807555306033136E-4	3.8957817773508773E-4
84-85	2.211483812377418	2.3807555306033136E-4	3.8957817773508773E-4
86-87	2.4441702006457477	2.3807555306033136E-4	3.8957817773508773E-4
88-89	2.6969415082995303	2.3807555306033136E-4	3.8957817773508773E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGTAG	6100	0.0	5.0955606	94-95
>>END_MODULE