##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib21_GTTTCG_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4620382 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.229160056462863 33.0 33.0 33.0 27.0 33.0 2 31.651021928489897 33.0 33.0 33.0 27.0 33.0 3 31.794492966166 33.0 33.0 33.0 27.0 33.0 4 35.62666160503612 37.0 37.0 37.0 33.0 37.0 5 35.137361802552256 37.0 37.0 37.0 33.0 37.0 6 34.92728047161469 37.0 33.0 37.0 33.0 37.0 7 34.730559724282536 37.0 33.0 37.0 27.0 37.0 8 34.76974717674859 37.0 33.0 37.0 27.0 37.0 9 34.679430618507304 37.0 33.0 37.0 27.0 37.0 10-11 34.53879343742574 37.0 33.0 37.0 27.0 37.0 12-13 34.538843000427235 37.0 33.0 37.0 27.0 37.0 14-15 35.46628709487657 37.0 33.0 40.0 27.0 40.0 16-17 35.365412859802504 37.0 33.0 40.0 27.0 40.0 18-19 35.577110940177676 37.0 33.0 40.0 27.0 40.0 20-21 35.25431122361744 37.0 33.0 40.0 27.0 40.0 22-23 35.278401980615456 37.0 33.0 40.0 27.0 40.0 24-25 35.11581942791743 37.0 33.0 40.0 27.0 40.0 26-27 34.73041677073454 37.0 33.0 40.0 27.0 40.0 28-29 34.36361452364761 37.0 33.0 40.0 27.0 40.0 30-31 34.24808056996153 37.0 33.0 40.0 24.5 40.0 32-33 34.25368183842808 37.0 33.0 40.0 24.5 40.0 34-35 34.096969796003876 37.0 33.0 38.5 24.5 40.0 36-37 33.78304001703755 37.0 33.0 37.0 22.0 40.0 38-39 33.68514746183324 37.0 33.0 37.0 22.0 40.0 40-41 33.38631048255317 37.0 33.0 37.0 22.0 40.0 42-43 33.001892917079154 37.0 33.0 37.0 22.0 40.0 44-45 32.87875190839199 37.0 33.0 37.0 22.0 40.0 46-47 32.727628148495086 37.0 33.0 37.0 22.0 40.0 48-49 32.74082727358907 37.0 33.0 37.0 22.0 40.0 50-51 33.03150551188192 37.0 33.0 37.0 22.0 40.0 52-53 32.88748646756913 37.0 33.0 37.0 22.0 40.0 54-55 32.52196517517383 37.0 33.0 37.0 22.0 40.0 56-57 32.56904991838337 37.0 33.0 37.0 22.0 40.0 58-59 32.28138257832361 37.0 30.0 37.0 22.0 40.0 60-61 32.182952405234026 33.0 27.0 37.0 22.0 40.0 62-63 32.17416373797664 33.0 27.0 37.0 22.0 40.0 64-65 31.83273460938944 33.0 27.0 37.0 22.0 40.0 66-67 31.48268660470065 33.0 27.0 37.0 18.5 40.0 68-69 31.316113472868693 33.0 27.0 37.0 15.0 40.0 70-71 31.157953281784927 33.0 27.0 37.0 15.0 38.5 72-73 30.937916929812296 33.0 27.0 37.0 15.0 37.0 74-75 30.591489513204753 33.0 27.0 37.0 15.0 37.0 76-77 29.521427016207753 33.0 27.0 35.0 15.0 37.0 78-79 30.44947484861641 33.0 27.0 37.0 15.0 37.0 80-81 30.17223478491605 33.0 27.0 37.0 15.0 37.0 82-83 30.151989489180764 33.0 27.0 37.0 15.0 37.0 84-85 30.0176303604334 33.0 27.0 37.0 15.0 37.0 86-87 29.821529367052335 33.0 27.0 37.0 15.0 37.0 88-89 29.681928031058902 33.0 27.0 37.0 15.0 37.0 90-91 29.588468118004094 33.0 27.0 37.0 15.0 37.0 92-93 29.38031498261399 33.0 27.0 33.0 15.0 37.0 94-95 29.28545302098398 33.0 27.0 33.0 15.0 37.0 96-97 29.138461928039717 33.0 27.0 33.0 15.0 37.0 98-99 28.879749120310834 33.0 27.0 33.0 15.0 37.0 100-101 28.075985167460182 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.0836424892643208 2306 2 0.537577463924297 2306 3 0.4941541676616197 2306 4 0.3397986173310983 2306 5 0.6380469012952759 2306 6 0.6778372889201947 2306 7 0.8433610045787034 2306 8 0.7515980956835335 2306 9 0.9837136767700088 2306 10-11 1.128316133857183 2306 12-13 1.3811478516491533 2306 14-15 1.4753495856986376 2306 16-17 1.347689202953923 2306 18-19 1.2234437426454718 2306 20-21 1.5368942664598535 2306 22-23 1.1662852805095056 2306 24-25 1.1620809146211286 2306 26-27 1.2831535669623477 2306 28-29 1.5693318220584374 2306 30-31 1.4628715445473262 2306 32-33 1.2724952904013094 2306 34-35 1.1343462115104543 2306 36-37 1.1735131721289491 2306 38-39 0.9231778239398665 2306 40-41 1.0606368972106566 2306 42-43 1.0658636261718328 2306 44-45 0.9540769107367808 2306 46-47 0.7362819331987112 2306 48-49 0.913892374768011 2306 50-51 1.0028899273091767 2306 52-53 1.2030979580465555 2306 54-55 1.5723389920423756 2306 56-57 1.1439500656263064 2306 58-59 1.0583987606600012 2306 60-61 0.8882984551290534 2306 62-63 0.6002904451202475 2306 64-65 0.722321660030218 2306 66-67 0.914384764244268 2306 68-69 0.7424598037809176 2306 70-71 0.7478185584307795 2306 72-73 0.40935663795240984 2306 74-75 0.7420079001629531 2306 76-77 0.7006446684141103 2306 78-79 0.700018528663378 2306 80-81 1.2297670290059912 2306 82-83 1.1147898578670024 2306 84-85 0.8835666548059393 2306 86-87 0.6744939737616846 2306 88-89 0.4664288746399805 2306 90-91 0.22517561143377662 2306 92-93 0.04287514114587054 2306 94-95 -0.01686178504964886 2306 96-97 0.06855252790022703 2306 98-99 0.29952015141455135 2306 100-101 0.17510770085905136 2307 1 0.5659093487275477 2307 2 0.44833675239311077 2307 3 0.3350683541399988 2307 4 0.2815198982039817 2307 5 0.49659292498785135 2307 6 0.6036469189380895 2307 7 0.7911110127875531 2307 8 0.7620165039444373 2307 9 0.9466456600409785 2307 10-11 1.0984734884419183 2307 12-13 1.3447725647953561 2307 14-15 1.329959908422076 2307 16-17 1.3496415478278934 2307 18-19 1.252563522575258 2307 20-21 1.5025396951018166 2307 22-23 1.2053654755275218 2307 24-25 1.241243295455952 2307 26-27 1.387996133329402 2307 28-29 1.5745840770606705 2307 30-31 1.6430966981744888 2307 32-33 1.2922961375549065 2307 34-35 1.2794938066814652 2307 36-37 1.3182800467937525 2307 38-39 0.9637010501284138 2307 40-41 1.1207408273660917 2307 42-43 1.196919673785608 2307 44-45 1.004349286549008 2307 46-47 1.0159429258253425 2307 48-49 1.0753866778242553 2307 50-51 0.9522325015810438 2307 52-53 1.2203244360352699 2307 54-55 1.5202121452025423 2307 56-57 1.2175146656484443 2307 58-59 1.1902825038481772 2307 60-61 0.9176039861931713 2307 62-63 0.8745184177282148 2307 64-65 0.8657709347932823 2307 66-67 1.0904655053030012 2307 68-69 0.8898048419247218 2307 70-71 0.8647108935514147 2307 72-73 0.7563526721644713 2307 74-75 0.892835802478821 2307 76-77 0.9355358676727583 2307 78-79 0.9862659641569991 2307 80-81 1.5120899275701127 2307 82-83 1.3071722858145591 2307 84-85 1.1031965145987357 2307 86-87 1.075617747450309 2307 88-89 0.955844659716405 2307 90-91 0.7728901573990967 2307 92-93 0.7745533833082412 2307 94-95 0.6644927364472082 2307 96-97 0.6136573397472418 2307 98-99 0.6205839214304696 2307 100-101 0.5960324676364266 1101 1 0.9864260360013688 1101 2 0.5771245013655353 1101 3 0.35439489335134056 1101 4 0.3440968923766121 1101 5 0.594379789288908 1101 6 0.6121134827071657 1101 7 0.7056816079796775 1101 8 0.6969534852427159 1101 9 0.9030966127900797 1101 10-11 0.9138893078533812 1101 12-13 1.1811145177909452 1101 14-15 1.2700450690335288 1101 16-17 1.2186243315351604 1101 18-19 0.9101432667416631 1101 20-21 1.3516151620165928 1101 22-23 0.9733616031744674 1101 24-25 1.0583669753270257 1101 26-27 1.014382304817964 1101 28-29 1.3393653376055568 1101 30-31 1.303146185499763 1101 32-33 0.9261366389206103 1101 34-35 0.9496407837360366 1101 36-37 1.0544254926960122 1101 38-39 0.8471870924966609 1101 40-41 1.017971084885616 1101 42-43 1.045062353864978 1101 44-45 0.8249102450795007 1101 46-47 0.6946187529403005 1101 48-49 0.720372103736608 1101 50-51 0.6811068081466871 1101 52-53 0.9890089038342182 1101 54-55 1.3759084765424987 1101 56-57 1.0454104568388587 1101 58-59 1.0965767358461456 1101 60-61 0.8318940543814577 1101 62-63 0.58669987259427 1101 64-65 0.5957563192484443 1101 66-67 0.708084761942871 1101 68-69 0.6400884970621803 1101 70-71 0.6837591921859172 1101 72-73 0.5431881541947909 1101 74-75 0.7351942996581009 1101 76-77 0.8143631014805237 1101 78-79 0.7786688707835907 1101 80-81 1.3915649739833214 1101 82-83 1.1206820291342368 1101 84-85 1.0058905286072495 1101 86-87 0.7981184252125928 1101 88-89 0.7380131171244173 1101 90-91 0.6062714135721343 1101 92-93 0.6888989036324418 1101 94-95 0.6683110192701847 1101 96-97 0.6395667032687804 1101 98-99 0.6672155245248312 1101 100-101 0.7894732613632733 2201 1 1.4058264973762995 2201 2 0.7866252387958106 2201 3 0.2621620995057192 2201 4 0.2275414853897857 2201 5 0.3990907973755924 2201 6 0.46772089826170316 2201 7 0.6105837406390506 2201 8 0.6598960704542591 2201 9 0.6856199577537794 2201 10-11 0.8530321937611092 2201 12-13 1.0508886447209775 2201 14-15 1.10424634029485 2201 16-17 1.0413151017661306 2201 18-19 0.8915138115106629 2201 20-21 1.188702699012623 2201 22-23 0.9468884707222642 2201 24-25 0.8129319309070127 2201 26-27 0.9820443325365602 2201 28-29 1.3472407551485546 2201 30-31 1.4038642439123734 2201 32-33 1.0485591005304684 2201 34-35 0.8765084124356619 2201 36-37 1.067185911114052 2201 38-39 0.764217916611841 2201 40-41 1.0371068986228664 2201 42-43 1.0573398258669187 2201 44-45 0.8649441000743181 2201 46-47 0.8519440799118883 2201 48-49 1.0095294591251118 2201 50-51 0.9087645974173455 2201 52-53 1.079590892493563 2201 54-55 1.37548491491836 2201 56-57 1.0544909548357637 2201 58-59 1.1600250677144963 2201 60-61 0.963835128527144 2201 62-63 0.7613734521820028 2201 64-65 0.8298954599802428 2201 66-67 0.8341163767596811 2201 68-69 0.8461419533870291 2201 70-71 0.9285235889400383 2201 72-73 0.788397855982911 2201 74-75 0.7822395801068751 2201 76-77 0.8187425579630343 2201 78-79 0.7939856720124681 2201 80-81 1.3435063545593877 2201 82-83 1.1897386134255719 2201 84-85 1.0487755767110016 2201 86-87 1.028871449556565 2201 88-89 1.0291111830885669 2201 90-91 0.840672855675983 2201 92-93 0.924607018480323 2201 94-95 0.8543488173059686 2201 96-97 0.6666221292937315 2201 98-99 0.8467460104794249 2201 100-101 0.8122956702428361 2304 1 -1.8039702323328228 2304 2 -0.26680485278790655 2304 3 -0.16052155327813722 2304 4 0.025695711916334574 2304 5 0.39416055621715884 2304 6 0.5572630481893555 2304 7 0.4893837023699774 2304 8 0.62554071171364 2304 9 0.920414177165334 2304 10-11 0.8724133908912108 2304 12-13 1.1257515133176241 2304 14-15 1.16908468312009 2304 16-17 1.1505077850468624 2304 18-19 0.8700240352568898 2304 20-21 1.1181194869271636 2304 22-23 0.9311698804816757 2304 24-25 1.0236219878504897 2304 26-27 1.0415477795160726 2304 28-29 1.4858109836672213 2304 30-31 1.4905903853675682 2304 32-33 0.9350289115316173 2304 34-35 0.8435889490532489 2304 36-37 1.0606214942513787 2304 38-39 0.9306747275104925 2304 40-41 1.006604030483139 2304 42-43 0.991158111643216 2304 44-45 0.9541859544082385 2304 46-47 0.8919989119967724 2304 48-49 0.831561435184085 2304 50-51 0.8691873690029794 2304 52-53 1.0623564006173893 2304 54-55 1.515468688550655 2304 56-57 1.038362481328619 2304 58-59 0.9673584128357398 2304 60-61 0.5472754041211729 2304 62-63 0.411057566793648 2304 64-65 0.42105998267399514 2304 66-67 0.4813815007696718 2304 68-69 0.5888165303360502 2304 70-71 0.5219647135986811 2304 72-73 0.29398525614747584 2304 74-75 0.5920589021721234 2304 76-77 0.6483703267774601 2304 78-79 0.6196708216252382 2304 80-81 1.1964649905605995 2304 82-83 0.9027579122790179 2304 84-85 0.5746401155296468 2304 86-87 0.43479617292834405 2304 88-89 0.35065138937559226 2304 90-91 0.2715181661368469 2304 92-93 0.2221944850452644 2304 94-95 -0.025355746588218153 2304 96-97 0.08195467008949464 2304 98-99 0.42021275826719773 2304 100-101 0.4859955701670593 1102 1 1.4088135096016536 1102 2 0.1128501571399525 1102 3 0.2770575329760483 1102 4 0.1826505264177527 1102 5 0.27457255916645806 1102 6 0.1767665145700832 1102 7 -4.829708271260284 1102 8 -1.1706621769683636 1102 9 0.20032235876887228 1102 10-11 0.6589911249735394 1102 12-13 0.9386162493165386 1102 14-15 0.8273400988009669 1102 16-17 0.6388619531901298 1102 18-19 0.8117042577035818 1102 20-21 1.1601525365058833 1102 22-23 0.7167738667579115 1102 24-25 0.9967008464719314 1102 26-27 1.0716275372180064 1102 28-29 0.8859767548044886 1102 30-31 1.15503003413626 1102 32-33 1.0243717348530978 1102 34-35 1.2152057619020198 1102 36-37 1.2462553097660916 1102 38-39 0.9360955683500549 1102 40-41 1.1419620052604742 1102 42-43 1.1983153170928915 1102 44-45 1.0960574796751814 1102 46-47 0.9182250669050234 1102 48-49 0.9013873269340991 1102 50-51 0.6955843825425276 1102 52-53 0.9408763051116509 1102 54-55 1.378985384734932 1102 56-57 1.0962377191617136 1102 58-59 1.0234775013597321 1102 60-61 0.8727204402706477 1102 62-63 0.6837320348642599 1102 64-65 0.49692120772226644 1102 66-67 0.6157166211033989 1102 68-69 0.636145342322127 1102 70-71 0.615872091639055 1102 72-73 0.5272356386878876 1102 74-75 0.6685190962998782 1102 76-77 0.6367144648755314 1102 78-79 0.6724274679433755 1102 80-81 1.3009228383535039 1102 82-83 1.179207793723016 1102 84-85 0.7573928440425561 1102 86-87 0.6281082779319007 1102 88-89 0.4967323231438101 1102 90-91 0.10741891565082184 1102 92-93 0.3096948818263172 1102 94-95 0.30811971770359037 1102 96-97 0.22076580680234414 1102 98-99 0.2233667799022392 1102 100-101 0.39932895712924577 2202 1 1.5409189026276628 2202 2 0.8861395878487848 2202 3 0.4054319347359723 2202 4 -0.18689566305366867 2202 5 0.07181071563167762 2202 6 0.5040533416031892 2202 7 0.6746475480885934 2202 8 -4.1813660269116255 2202 9 -0.5101270735881371 2202 10-11 0.5303381000022185 2202 12-13 0.09953117262934086 2202 14-15 0.131582375233819 2202 16-17 0.26204500005709264 2202 18-19 0.7903388043090729 2202 20-21 1.2572311569809287 2202 22-23 -2.7458795024853444 2202 24-25 -2.7999674079182952 2202 26-27 -1.3373650553675205 2202 28-29 0.6820574639791914 2202 30-31 1.2943352284899987 2202 32-33 1.222462497276993 2202 34-35 -2.0531791621059945 2202 36-37 -0.23688546833618318 2202 38-39 0.31083378089469704 2202 40-41 1.1568922305374514 2202 42-43 1.2785593428852877 2202 44-45 1.3769929288490133 2202 46-47 0.7783776999568062 2202 48-49 1.0276708097250378 2202 50-51 -3.89424609694521 2202 52-53 -0.21403971178949632 2202 54-55 1.531475366406113 2202 56-57 1.324853198766391 2202 58-59 1.4219003891425182 2202 60-61 1.3449806983886745 2202 62-63 1.2294785266395216 2202 64-65 1.1820211619200975 2202 66-67 1.3820857884664832 2202 68-69 1.2875648992263464 2202 70-71 1.3044562558624833 2202 72-73 1.1580954959932264 2202 74-75 0.810713040709512 2202 76-77 1.291470428742251 2202 78-79 1.3530747564281356 2202 80-81 1.8954277080352888 2202 82-83 1.781784941099236 2202 84-85 1.5422456590461948 2202 86-87 1.532010052889106 2202 88-89 1.550909881546346 2202 90-91 1.2160295159487298 2202 92-93 1.3236050344911874 2202 94-95 1.2806334229555958 2202 96-97 1.1948861753387554 2202 98-99 1.3807280422156474 2202 100-101 1.3678762091663614 2305 1 1.2997132505840767 2305 2 0.693053401580908 2305 3 0.14949088173676017 2305 4 -0.11679462924991668 2305 5 0.2680470485066806 2305 6 0.36648700835446846 2305 7 0.4469298953536054 2305 8 -0.08201158545017506 2305 9 0.41851502778663274 2305 10-11 0.22167833982641127 2305 12-13 0.842962981012775 2305 14-15 0.5550254325962243 2305 16-17 0.6796269978167615 2305 18-19 0.6800489901558393 2305 20-21 0.7635537773723797 2305 22-23 0.4602515588580971 2305 24-25 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-2.885202745336052 1208 38-39 -0.48441383642629887 1208 40-41 -0.9096143852209835 1208 42-43 0.15755011948691333 1208 44-45 0.046885400147154144 1208 46-47 -0.13414920070054848 1208 48-49 -1.561128046041837 1208 50-51 -0.35261239829448243 1208 52-53 -1.658201484957015 1208 54-55 -0.9489812904753983 1208 56-57 -6.758294273490733 1208 58-59 -2.226990742062771 1208 60-61 -1.538468584942155 1208 62-63 0.2890363298545289 1208 64-65 -0.03244140115325678 1208 66-67 0.6607897087838808 1208 68-69 0.35939858441811623 1208 70-71 -4.238021374480816 1208 72-73 -0.6579583306088743 1208 74-75 -5.553195386785912 1208 76-77 -1.1681760806599542 1208 78-79 -3.881375924341242 1208 80-81 0.8085900749725532 1208 82-83 0.3589075401110655 1208 84-85 -2.699402545301247 1208 86-87 -1.0280629461054787 1208 88-89 -4.740869357709837 1208 90-91 -0.07097339707429384 1208 92-93 -2.23829055572174 1208 94-95 -4.732970167271702 1208 96-97 -3.572319501691407 1208 98-99 -6.517215796867088 1208 100-101 -4.074322717653903 1209 1 1.716902794682884 1209 2 1.1267675948669584 1209 3 0.5489190676197779 1209 4 0.4098962698746149 1209 5 0.4366805430652363 1209 6 1.0345978126908548 1209 7 0.7999861729155953 1209 8 0.18109442256690755 1209 9 -0.01307620449674829 1209 10-11 -3.09391553885704 1209 12-13 -0.46456757726981124 1209 14-15 -1.7296458529147003 1209 16-17 -0.4417674318630631 1209 18-19 -16.713840963333716 1209 20-21 -14.11055426074956 1209 22-23 -3.363922714149446 1209 24-25 -2.0708648265984877 1209 26-27 -1.4518565275349502 1209 28-29 -3.525497783284443 1209 30-31 -0.8117520095845592 1209 32-33 -0.8090677904895642 1209 34-35 -2.727468923147619 1209 36-37 -4.008516885008426 1209 38-39 -1.7987368609814993 1209 40-41 -10.16639106786215 1209 42-43 -4.950847876982273 1209 44-45 -2.9583589154686685 1209 46-47 -2.759932413105421 1209 48-49 -3.286618454809897 1209 50-51 -2.257823752506635 1209 52-53 -1.4524605088702458 1209 54-55 -2.9274805645313933 1209 56-57 -1.1079994870377021 1209 58-59 -2.292222479625611 1209 60-61 -2.148232741783385 1209 62-63 -1.544754334206953 1209 64-65 -0.4488195097425347 1209 66-67 -0.1403505038486621 1209 68-69 -1.4360059240860679 1209 70-71 -1.4511856645672978 1209 72-73 -0.8382186020634137 1209 74-75 -0.05701790653942851 1209 76-77 -16.657393409447273 1209 78-79 -16.65246142524261 1209 80-81 -21.792468119050447 1209 82-83 -10.087961149867919 1209 84-85 -3.698211805846096 1209 86-87 -2.532490458077021 1209 88-89 -2.050650100225525 1209 90-91 -5.883383470527139 1209 92-93 -3.4347808777124342 1209 94-95 -1.0823581302242964 1209 96-97 -0.289660185907632 1209 98-99 0.7683356313204435 1209 100-101 -0.045250899584054594 1212 1 -28.632483418725993 1212 2 -16.298324473121706 1212 3 -5.715712660425545 1212 4 -1.3995536829110335 1212 5 -0.3239143577846235 1212 6 -2.49202045926382 1212 7 -0.27618651358110213 1212 8 -1.1062302941469753 1212 9 0.017952478222795776 1212 10-11 0.8117513638813918 1212 12-13 0.25728056956683076 1212 14-15 -0.18376058379288907 1212 16-17 -0.025160373317262952 1212 18-19 -1.000010403843632 1212 20-21 -0.6897280095692011 1212 22-23 -0.3694146286914659 1212 24-25 0.2756478032409859 1212 26-27 -0.5818842659674388 1212 28-29 0.39992655571461455 1212 30-31 -0.747975156421198 1212 32-33 0.01944141630929863 1212 34-35 -1.1906553018067356 1212 36-37 -1.3714391583795305 1212 38-39 -0.44334911310614444 1212 40-41 -0.46541107494429923 1212 42-43 0.36313435861735144 1212 44-45 0.9413937993566392 1212 46-47 -2.09962227382308 1212 48-49 -1.506692522326425 1212 50-51 -0.20217514533005243 1212 52-53 -1.0255816727040497 1212 54-55 -0.3239170612263855 1212 56-57 -1.3695245106448795 1212 58-59 -0.37100877565960744 1212 60-61 0.030209774741638995 1212 62-63 -4.85539054761583 1212 64-65 -4.406318624473414 1212 66-67 -1.7784852418090011 1212 68-69 0.10005816939835199 1212 70-71 -0.6684079851527542 1212 72-73 -1.3118812555100625 1212 74-75 -0.3625222148491538 1212 76-77 0.9653034153874742 1212 78-79 -1.3445577425230617 1212 80-81 0.4612296500713633 1212 82-83 0.4738568978185782 1212 84-85 -3.0898867350245673 1212 86-87 -2.118275396698362 1212 88-89 -0.8966870454568721 1212 90-91 -0.08628095636283106 1212 92-93 -2.4505472256822145 1212 94-95 -2.0876638431610317 1212 96-97 -0.042240450308010935 1212 98-99 -0.6767192553651107 1212 100-101 -0.05076169986734058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 5.0 10 26.0 11 38.0 12 130.0 13 527.0 14 1534.0 15 3425.0 16 6461.0 17 10747.0 18 16284.0 19 23682.0 20 33072.0 21 43620.0 22 57007.0 23 73459.0 24 91355.0 25 112229.0 26 137172.0 27 164072.0 28 195479.0 29 230429.0 30 269457.0 31 312350.0 32 358634.0 33 407276.0 34 452431.0 35 490971.0 36 511085.0 37 456229.0 38 160771.0 39 421.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.39524436638414 26.419242242539408 23.095263939583766 24.090249451492685 2 26.534740941244 28.786665414202684 22.867128144813694 21.811465499739626 3 18.92026782282277 25.40827916737368 30.881825863221284 24.789627146582262 4 22.90519890251427 26.928050707528538 28.53400121505184 21.63274917490535 5 22.117630100714617 27.70894268049698 27.500994506514832 22.672432712273576 6 21.314060179439707 29.365753740708016 26.83866831790099 22.481517761951284 7 22.039930897488563 28.599431821871008 27.897368659128187 21.463268621512245 8 21.86068164926623 28.059130175816634 27.957385341731484 22.122802833185652 9 21.020837670997768 26.26886694649923 29.85699017959987 22.853305202903137 10-11 22.1966603627146 26.78402997847364 28.04975865631889 22.969551002492867 12-13 22.456668214517613 27.134090909337317 27.492835994332328 22.916404881812742 14-15 22.585650683964232 27.2822552168593 27.082434011657696 23.049660087518767 16-17 22.72102862913585 27.10541357897637 27.162129901914632 23.011427889973145 18-19 22.78397096464024 27.159426334962962 27.059850218713184 22.996752481683618 20-21 22.80204814278303 27.07960663850016 27.02202480704513 23.096320411671677 22-23 22.66685958000875 27.02717004784453 27.268924950361246 23.03704542178547 24-25 22.560504737487076 27.128070795877917 27.38918556950486 22.92223889713015 26-27 22.765974761394187 27.19413676185216 27.142799015319515 22.897089461434142 28-29 22.85689797943114 27.107185076904894 27.145244700546407 22.89067224311756 30-31 22.817420724087313 27.136013862057297 27.071538673642138 22.975026740213256 32-33 22.815591870975172 27.047655367023765 27.025460232508912 23.11129252949215 34-35 22.96010373168279 27.00629515048756 27.068367940140014 22.965233177689637 36-37 22.80167527273719 27.198000078781366 27.17920293170565 22.8211217167758 38-39 22.893236966120984 27.02922615489369 27.180393309470947 22.897143569514384 40-41 22.91779957801318 26.960481779647218 27.061882417897674 23.059836224441927 42-43 22.646969449712167 27.136262759226405 27.3331295983752 22.88363819268623 44-45 22.7968055455155 26.93185325369201 27.258373874714252 23.012967326078236 46-47 22.82464287330177 27.0056344047008 27.227681083211973 22.94204163878545 48-49 22.849518248238372 27.043601956766523 27.267826059667204 22.8390537353279 50-51 22.940001242322328 27.02367056901609 27.071545429498318 22.964782759163263 52-53 22.827430367943037 27.024416089125225 27.268844274669885 22.879309268261856 54-55 22.688842186370465 27.011769900395215 27.428856817581693 22.870531095652627 56-57 22.882734244030885 27.081526769501878 27.136934373048355 22.89880461341888 58-59 22.826004184886767 26.94858451626559 27.33475010428637 22.89066119456127 60-61 22.874468581298064 27.00555537819805 27.167319853321132 22.95265618718275 62-63 22.947607730607658 26.97242468280534 27.102675697374817 22.977291889212186 64-65 22.941766709716184 26.93304555738301 27.169385019590887 22.955802713309918 66-67 22.88482212872392 27.123261994642185 27.153780372008196 22.838135504625697 68-69 23.026207923836214 26.999575879433312 27.028449410229992 22.945766786500478 70-71 23.108818091660847 26.996382845267075 26.93483797042816 22.959961092643923 72-73 22.82681134599321 27.107226850145782 27.227566815962128 22.838394987898884 74-75 22.928285448605294 26.91963037292372 27.162402084476195 22.98968209399479 76-77 22.90041668455059 27.119017177669942 27.07506608380254 22.90550005397693 78-79 22.991392140522063 27.14424092180486 27.0113619676171 22.853004970055974 80-81 23.015250390764113 27.001602276404764 26.914776641759097 23.06837069107203 82-83 22.990646539087432 27.02291900402183 26.94542171307215 23.041012743818587 84-85 22.90219874527476 27.010725877057705 27.082206472321698 23.00486890534584 86-87 22.996631859736798 27.06455786151556 26.89888890981215 23.03992136893549 88-89 23.021649692616464 27.026526271132788 26.867068863544002 23.084755172706746 90-91 23.01691126473071 27.08610113074424 26.804697720262194 23.09228988426286 92-93 23.007631370352204 26.95676162877028 26.827418386372976 23.20818861450454 94-95 23.07317990241987 26.889009123013764 26.78973840347097 23.248072571095403 96-97 22.999227472957763 27.074280563899283 26.811959038966837 23.114532924176114 98-99 23.068353004496807 26.95863389881908 26.713960578882336 23.259052517801777 100-101 23.13567453048436 26.779348617225356 26.663867115099993 23.421109737190292 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 2.0 3 4.5 4 5.5 5 8.5 6 21.0 7 39.0 8 54.0 9 91.5 10 153.5 11 249.0 12 385.5 13 589.0 14 933.0 15 1458.5 16 2230.0 17 3426.0 18 5235.5 19 7710.5 20 10957.0 21 15103.5 22 20145.5 23 26089.0 24 33154.0 25 40916.5 26 49398.5 27 58660.0 28 68494.0 29 78342.5 30 87617.5 31 96873.0 32 106512.0 33 115774.0 34 123961.5 35 131701.5 36 139086.5 37 145469.0 38 149253.5 39 150332.0 40 149791.0 41 146643.5 42 141216.5 43 134836.5 44 127310.5 45 119368.0 46 111994.5 47 105526.0 48 99188.5 49 92271.0 50 86220.5 51 81040.5 52 76660.5 53 74272.5 54 73283.5 55 73202.5 56 74438.0 57 76058.0 58 77868.5 59 79407.0 60 79897.5 61 79665.0 62 78668.5 63 77190.5 64 74783.5 65 71305.5 66 67134.5 67 62448.0 68 57336.0 69 51791.5 70 46258.0 71 40424.5 72 34886.0 73 29485.0 74 24135.0 75 19383.5 76 15123.0 77 11533.5 78 8466.5 79 6104.5 80 4302.0 81 2913.5 82 1959.5 83 1338.5 84 886.0 85 608.5 86 420.5 87 289.0 88 190.0 89 136.5 90 110.5 91 70.5 92 43.0 93 29.5 94 14.0 95 6.5 96 4.5 97 3.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7028856055624838 2 0.0033763442070374266 3 3.030052493495127E-4 4 6.492969628918129E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.1863806931980949 14-15 3.2464848144590644E-5 16-17 4.1122140983148144E-4 18-19 0.11513117313676662 20-21 2.1643232096393763E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.002066928665205604 42-43 0.0 44-45 0.0 46-47 0.023439620360394445 48-49 9.739454443377192E-5 50-51 6.492969628918129E-5 52-53 1.4068100862655946E-4 54-55 0.002640474315760039 56-57 0.0016773504874705164 58-59 0.09710236080047062 60-61 0.0018288531121452727 62-63 0.0015691343269885478 64-65 0.002618831083663645 66-67 0.004653294900724659 68-69 0.005053694694507943 70-71 0.003809208848965302 72-73 0.03895781777350878 74-75 0.0444010906457518 76-77 0.15799559430367446 78-79 2.488971691085282E-4 80-81 0.11655962645512861 82-83 0.004772332677254825 84-85 0.005345878327809259 86-87 0.007034050431327973 88-89 0.007347877296725681 90-91 0.022400745219767544 92-93 0.010908188976582456 94-95 0.014966294994656284 96-97 0.010367108174172612 98-99 0.011871312804871978 100-101 0.012866901481306092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4620382.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.12076044455432 #Duplication Level Percentage of deduplicated Percentage of total 1 98.65823908945669 96.80421443578143 2 1.0454467920241874 2.051600684754662 3 0.16163166978645127 0.4757826705410908 4 0.07662867129572232 0.30075453997568263 5 0.025565003641356596 0.12542287990288545 6 0.017058086396320716 0.10042514453615374 7 0.005487700348140762 0.037692013187599196 8 0.004805891199720773 0.0377246159304315 9 0.004275597319330164 0.03775723742946362 >10 8.065340631212748E-4 0.012662037695663307 >50 0.0 0.0 >100 5.4964468991900055E-5 0.015963740264952425 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.3807555306033136E-4 0.0 2.1643232096393763E-5 2 2.3807555306033136E-4 0.0 4.3286464192787526E-5 3 3.8957817773508773E-4 0.0 4.3286464192787526E-5 4 4.977943382170565E-4 0.0 6.492969628918129E-5 5 6.492969628918129E-4 0.0 6.492969628918129E-5 6 8.224428196629629E-4 0.0 6.492969628918129E-5 7 9.739454443377192E-4 0.0 6.492969628918129E-5 8 0.0012985939257836258 0.0 6.492969628918129E-5 9 0.0014284533183619883 0.0 6.492969628918129E-5 10-11 0.0016340640232777289 0.0 7.575131233737817E-5 12-13 0.0019587125047236353 0.0 8.657292838557505E-5 14-15 0.0022617177540731484 0.0 1.082161604819688E-4 16-17 0.0025322581552780703 0.0 1.731458567711501E-4 18-19 0.0027595120922902045 0.0 1.9478908886754387E-4 20-21 0.003094982189784308 0.0 1.9478908886754387E-4 22-23 0.0033114145107482453 0.0 2.164323209639376E-4 24-25 0.0034845603675193954 0.0 2.2725393701213448E-4 26-27 0.0038200304650134985 0.0 2.3807555306033136E-4 28-29 0.004350289651375147 0.0 2.3807555306033136E-4 30-31 0.005432451256194834 0.0 2.3807555306033136E-4 32-33 0.00742362860906306 0.0 2.3807555306033136E-4 34-35 0.010107389389015887 0.0 2.3807555306033136E-4 36-37 0.013927419854029385 0.0 2.3807555306033136E-4 38-39 0.019900951912634066 0.0 2.3807555306033136E-4 40-41 0.028125380109263694 0.0 2.5971878515672515E-4 42-43 0.039931763217846486 1.0821616048196881E-5 3.2464848144590644E-4 44-45 0.05504956083717753 2.1643232096393763E-5 3.8957817773508773E-4 46-47 0.07618417697930605 4.3286464192787526E-5 3.8957817773508773E-4 48-49 0.10259974175295462 4.3286464192787526E-5 3.8957817773508773E-4 50-51 0.13612510827026858 6.492969628918129E-5 3.8957817773508773E-4 52-53 0.17619755249674163 8.657292838557505E-5 4.1122140983148144E-4 54-55 0.2227629663521328 8.657292838557505E-5 4.1122140983148144E-4 56-57 0.2777259542609247 8.657292838557505E-5 4.1122140983148144E-4 58-59 0.34398670932403425 8.657292838557505E-5 4.1122140983148144E-4 60-61 0.42392598707206464 8.657292838557505E-5 4.328646419278752E-4 62-63 0.5101742669761937 8.657292838557505E-5 4.5450787402426897E-4 64-65 0.6089431566480867 8.657292838557505E-5 4.5450787402426897E-4 66-67 0.7179384734855256 1.082161604819688E-4 4.5450787402426897E-4 68-69 0.842397879655838 1.082161604819688E-4 4.5450787402426897E-4 70-71 0.985589503205579 1.190377765301657E-4 4.5450787402426897E-4 72-73 1.1355121719372987 1.2985939257836258E-4 4.5450787402426897E-4 74-75 1.297944628820734 1.2985939257836258E-4 4.8697272216885966E-4 76-77 1.475224342922295 1.2985939257836258E-4 4.977943382170565E-4 78-79 1.6732707382203462 1.2985939257836258E-4 4.977943382170565E-4 80-81 1.8941291001479965 1.2985939257836258E-4 5.194375703134503E-4 82-83 2.1278976500211453 1.5150262467475634E-4 5.410808024098441E-4 84-85 2.370702249294539 1.5150262467475634E-4 5.410808024098441E-4 86-87 2.623473556948322 1.6232424072295322E-4 5.410808024098441E-4 88-89 2.897314551047944 1.8396747281934698E-4 5.410808024098441E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTTC 3970 0.0 6.455829 94-95 CACGTTT 4405 0.0 6.1415462 94-95 AGTCACG 4415 0.0 6.075759 90-91 GTCACGT 4500 0.0 5.906644 92-93 CTCCAGT 7595 0.0 5.7179728 94-95 TCACGTT 4725 0.0 5.675601 92-93 TCTCGTA 1835 0.0 5.560978 94-95 CTCGTAT 1470 2.0008883E-11 5.4888372 94-95 TCCAGTC 7520 0.0 5.3647547 94-95 CGTTTCG 3835 0.0 5.197957 94-95 TCGTATG 1700 9.094947E-12 5.1652107 92-93 CACGTCT 11070 0.0 5.1235275 94-95 GTTTCGA 3510 0.0 5.0707583 94-95 CAGTCAC 5720 0.0 5.061539 94-95 CGTCTGA 9710 0.0 5.010181 94-95 CCAGTCA 6500 0.0 5.0017962 94-95 >>END_MODULE