##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib20_GTGGCC_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1423941 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.85095730792217 27.0 22.0 33.0 2.0 33.0 2 24.957955420905783 33.0 15.0 33.0 2.0 33.0 3 25.795558945209105 33.0 22.0 33.0 6.0 33.0 4 27.88829593360961 33.0 22.0 37.0 2.0 37.0 5 27.917670746189625 33.0 15.0 37.0 6.0 37.0 6 28.97032952910268 33.0 22.0 37.0 15.0 37.0 7 28.654138759962667 33.0 27.0 37.0 6.0 37.0 8 28.4394585168908 33.0 22.0 37.0 6.0 37.0 9 29.166143821970152 33.0 27.0 37.0 15.0 37.0 10-11 28.77530635047379 33.0 24.5 37.0 10.5 37.0 12-13 29.074428996707027 33.0 27.0 37.0 10.5 37.0 14-15 29.402931722592438 33.0 27.0 37.0 6.0 40.0 16-17 30.215013122032445 33.0 27.0 37.0 15.0 40.0 18-19 30.53548637197749 33.0 27.0 37.0 15.0 40.0 20-21 30.541748920776914 33.0 27.0 37.0 15.0 40.0 22-23 30.543273211460306 33.0 27.0 37.0 15.0 40.0 24-25 30.142910064391714 33.0 27.0 37.0 10.5 40.0 26-27 29.630162345209527 33.0 27.0 37.0 10.5 40.0 28-29 29.788930510463565 33.0 27.0 37.0 15.0 40.0 30-31 29.69927511041539 33.0 27.0 37.0 15.0 40.0 32-33 29.547986187629967 33.0 27.0 37.0 15.0 40.0 34-35 29.57447780490905 33.0 27.0 37.0 15.0 40.0 36-37 29.82740928170479 33.0 27.0 37.0 15.0 40.0 38-39 29.84245590231618 33.0 27.0 37.0 15.0 40.0 40-41 29.89666952493116 33.0 27.0 37.0 15.0 40.0 42-43 29.783772642265376 33.0 27.0 37.0 15.0 40.0 44-45 29.528437273735356 33.0 27.0 37.0 15.0 40.0 46-47 29.144688930229552 33.0 22.0 37.0 10.5 40.0 48-49 28.97576198732953 33.0 22.0 37.0 10.5 40.0 50-51 28.19702220808306 33.0 22.0 37.0 10.5 38.5 52-53 27.90465440632723 33.0 22.0 37.0 6.0 37.0 54-55 28.826510016917837 33.0 22.0 37.0 15.0 40.0 56-57 28.725686317059484 33.0 22.0 37.0 10.5 40.0 58-59 28.89329508736668 33.0 22.0 37.0 15.0 40.0 60-61 28.635301954224225 33.0 22.0 37.0 10.5 40.0 62-63 28.477805260189854 33.0 22.0 37.0 15.0 40.0 64-65 28.426350178834657 33.0 22.0 37.0 10.5 40.0 66-67 28.29359046477347 33.0 22.0 37.0 15.0 40.0 68-69 27.99512795825108 33.0 22.0 37.0 10.5 37.0 70-71 27.894576741592523 33.0 22.0 37.0 10.5 37.0 72-73 27.653809743521677 33.0 22.0 37.0 15.0 37.0 74-75 27.598584843051782 33.0 22.0 37.0 15.0 37.0 76-77 27.213569593122187 27.0 22.0 33.0 6.0 37.0 78-79 26.66477403207015 27.0 22.0 33.0 6.0 37.0 80-81 26.543930190927853 27.0 22.0 33.0 6.0 37.0 82-83 26.118833575267516 27.0 22.0 33.0 6.0 37.0 84-85 26.209900550654837 27.0 22.0 33.0 6.0 37.0 86-87 25.913895660002765 27.0 22.0 33.0 6.0 37.0 88-89 25.549394602725815 27.0 22.0 33.0 6.0 37.0 90-91 25.417801720717364 27.0 22.0 33.0 6.0 37.0 92-93 25.312143550891506 27.0 22.0 33.0 6.0 37.0 94-95 25.10210254497904 27.0 18.5 33.0 6.0 37.0 96-97 24.96219681854796 27.0 18.5 33.0 6.0 37.0 98-99 24.73184528010641 27.0 15.0 33.0 6.0 37.0 100-101 24.239693568764437 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.396651328351435 2306 2 8.50559393759276 2306 3 7.385953645738596 2306 4 9.036793473309128 2306 5 8.816144839677435 2306 6 7.827147957884872 2306 7 8.155714837508416 2306 8 8.567405137946206 2306 9 7.533775064504052 2306 10-11 7.928614728737237 2306 12-13 7.478897820915272 2306 14-15 8.069731677906486 2306 16-17 6.758027175489168 2306 18-19 6.147020918301649 2306 20-21 6.143990064759912 2306 22-23 5.6692231964068185 2306 24-25 5.698513405669349 2306 26-27 6.336010085953937 2306 28-29 5.527501749295393 2306 30-31 5.517896227364119 2306 32-33 5.459224548545752 2306 34-35 4.637438392821739 2306 36-37 4.903922042392445 2306 38-39 4.408382478499288 2306 40-41 3.725905728815164 2306 42-43 3.4457849985370643 2306 44-45 3.282869101650242 2306 46-47 3.373882069030966 2306 48-49 3.2493156745086615 2306 50-51 3.1428565113784472 2306 52-53 3.620436988295271 2306 54-55 3.145870667728026 2306 56-57 3.496482932774807 2306 58-59 3.1675614810653094 2306 60-61 3.0509152475208197 2306 62-63 2.6510925812706674 2306 64-65 2.280597191145894 2306 66-67 2.211927779222531 2306 68-69 2.3913959101608775 2306 70-71 2.3943421212631044 2306 72-73 1.7519639014123385 2306 74-75 1.2648293275533007 2306 76-77 1.571300458636106 2306 78-79 1.8347350710175228 2306 80-81 1.668769419112504 2306 82-83 1.681067601409822 2306 84-85 1.2037580271345618 2306 86-87 1.2156557021061474 2306 88-89 1.1275923376348196 2306 90-91 1.0070598545807279 2306 92-93 0.8890766331476634 2306 94-95 1.189176130964718 2306 96-97 0.8764617062515931 2306 98-99 0.6328137264481946 2306 100-101 0.5230805761642472 2307 1 8.422371815851807 2307 2 8.589174733672074 2307 3 7.784370695760959 2307 4 9.336083890401508 2307 5 9.235958051698844 2307 6 7.729410342923938 2307 7 8.238779774840783 2307 8 8.757367350204266 2307 9 7.605763489156363 2307 10-11 7.917634222002022 2307 12-13 7.433572573064048 2307 14-15 8.379383957241028 2307 16-17 7.035413291901229 2307 18-19 6.560678021291579 2307 20-21 6.499939280980243 2307 22-23 6.254650424346018 2307 24-25 6.431078602366238 2307 26-27 7.1900293352448585 2307 28-29 6.2813074694879525 2307 30-31 6.220276808588068 2307 32-33 6.32278787748162 2307 34-35 5.983860305693671 2307 36-37 5.912955558463686 2307 38-39 5.3905466524260355 2307 40-41 4.774785572030524 2307 42-43 4.682010149699419 2307 44-45 4.544880756771946 2307 46-47 4.942701505701731 2307 48-49 4.778703589102168 2307 50-51 4.47928415590426 2307 52-53 4.987874998394897 2307 54-55 4.529613901907016 2307 56-57 4.963801618372262 2307 58-59 4.6446131404785795 2307 60-61 4.5613262014626095 2307 62-63 4.309692274190212 2307 64-65 4.14271899576865 2307 66-67 3.9055094156013013 2307 68-69 3.8779561666095823 2307 70-71 3.6154107050398174 2307 72-73 3.1509863827384414 2307 74-75 2.6597362715643165 2307 76-77 2.898789751746314 2307 78-79 3.237458532029855 2307 80-81 2.99512193255309 2307 82-83 3.1496649476273504 2307 84-85 2.322562414626038 2307 86-87 2.341206387751903 2307 88-89 2.4382695051744 2307 90-91 2.3959465330234657 2307 92-93 2.257545816377583 2307 94-95 2.3238028920180938 2307 96-97 2.0540968046789665 2307 98-99 1.9687608463737725 2307 100-101 1.7034662449114748 1101 1 8.150667024385644 1101 2 7.843190334443825 1101 3 7.1357962746287456 1101 4 8.694481286084514 1101 5 8.52539361406506 1101 6 7.274671003769978 1101 7 6.975574645118126 1101 8 7.980122527471 1101 9 7.360389894043767 1101 10-11 7.404572119081607 1101 12-13 7.149481584647923 1101 14-15 7.592644431127891 1101 16-17 6.684414896532957 1101 18-19 5.9338468609033725 1101 20-21 5.825656911321289 1101 22-23 5.300233407662027 1101 24-25 5.885214118022997 1101 26-27 6.357221976337577 1101 28-29 5.742430735980346 1101 30-31 5.703437280562852 1101 32-33 5.778380217861429 1101 34-35 5.5120404554248985 1101 36-37 5.261040152633292 1101 38-39 4.866892826758789 1101 40-41 4.418438657145202 1101 42-43 4.349315846460744 1101 44-45 4.3161815828826136 1101 46-47 4.49947093010725 1101 48-49 4.19621923555145 1101 50-51 3.808645517331243 1101 52-53 4.696821753575808 1101 54-55 4.337976652477071 1101 56-57 4.725833397182566 1101 58-59 4.187848359701864 1101 60-61 4.361473371110151 1101 62-63 3.845720915364268 1101 64-65 3.6381170989221587 1101 66-67 3.4854326409383987 1101 68-69 3.6254139315915808 1101 70-71 3.5708399114011655 1101 72-73 3.0002953166857083 1101 74-75 2.4705636357100964 1101 76-77 2.560015178072412 1101 78-79 3.2452083842987207 1101 80-81 2.9449675546582164 1101 82-83 3.1434350491528598 1101 84-85 2.357039840975201 1101 86-87 2.3853434725303018 1101 88-89 2.3004717931783247 1101 90-91 2.254299009944173 1101 92-93 1.9849956113143286 1101 94-95 2.1843559330334763 1101 96-97 2.261559571425849 1101 98-99 2.161359144863198 1101 100-101 1.773127657600476 2201 1 6.917209754882538 2201 2 7.773810358297098 2201 3 6.127277884220305 2201 4 8.255508071835695 2201 5 3.7314296444048303 2201 6 5.507629470482435 2201 7 6.6434275697885745 2201 8 7.648148090804074 2201 9 6.4412050378122 2201 10-11 3.812429771427219 2201 12-13 6.1834237817936355 2201 14-15 6.891978176066512 2201 16-17 5.860149535514623 2201 18-19 1.7737750221435427 2201 20-21 4.816750316379732 2201 22-23 4.976557714607175 2201 24-25 5.222990959770687 2201 26-27 5.968989290001467 2201 28-29 5.128133558204052 2201 30-31 5.051980947916363 2201 32-33 5.366069500502849 2201 34-35 4.887348436186894 2201 36-37 4.669168024262035 2201 38-39 4.172633883642138 2201 40-41 3.9115402910032913 2201 42-43 3.8588626818525107 2201 44-45 3.8147071805161303 2201 46-47 4.2540401723650625 2201 48-49 3.899944067086693 2201 50-51 3.569586428003806 2201 52-53 4.402511835045551 2201 54-55 4.1111231992492385 2201 56-57 4.217671338759413 2201 58-59 3.763382682045009 2201 60-61 3.7538771253140197 2201 62-63 3.5112758138390987 2201 64-65 3.2335852978680215 2201 66-67 3.0459463664869375 2201 68-69 3.160737496324778 2201 70-71 3.018581330712067 2201 72-73 2.427255421554136 2201 74-75 1.9584064541483635 2201 76-77 2.3979985973221467 2201 78-79 2.8739487853411276 2201 80-81 2.1998755137654804 2201 82-83 2.4626187792555037 2201 84-85 2.1419496824741024 2201 86-87 2.0432256620885845 2201 88-89 2.0929659063135766 2201 90-91 1.952274798379058 2201 92-93 1.9370127045049053 2201 94-95 1.8483170491450132 2201 96-97 1.8033965521771016 2201 98-99 1.6173991011569981 2201 100-101 1.2973639149017195 2304 1 1.6499465527292827 2304 2 4.88603710278079 2304 3 5.735249690797499 2304 4 4.1098311159803025 2304 5 6.211269952840855 2304 6 6.234844788585679 2304 7 7.042011363063676 2304 8 6.415573457150824 2304 9 5.537135438193008 2304 10-11 6.523317020610541 2304 12-13 6.276843224177554 2304 14-15 6.4408224123888935 2304 16-17 5.217021934215786 2304 18-19 5.04010860546299 2304 20-21 4.7354483925214765 2304 22-23 4.403287003067177 2304 24-25 4.61544076648077 2304 26-27 5.637404429792177 2304 28-29 4.820357560465364 2304 30-31 4.793361532981891 2304 32-33 4.933394736246829 2304 34-35 4.446140376939642 2304 36-37 4.538687187685419 2304 38-39 4.18334073707403 2304 40-41 3.7685601488325418 2304 42-43 3.0658832477034323 2304 44-45 2.9734751846901943 2304 46-47 3.830284098356099 2304 48-49 3.5300014522428533 2304 50-51 3.0637889559679365 2304 52-53 3.922112666045134 2304 54-55 3.4991685665545376 2304 56-57 4.19129235763527 2304 58-59 3.4707902770479 2304 60-61 3.529234512866566 2304 62-63 3.0536333462637657 2304 64-65 3.000129245317563 2304 66-67 2.859097133710108 2304 68-69 3.187490775826678 2304 70-71 2.89542695257839 2304 72-73 2.5774390714820825 2304 74-75 2.1363533356396935 2304 76-77 2.3017971063374425 2304 78-79 2.5696422980109013 2304 80-81 2.474671113356351 2304 82-83 2.70786189483405 2304 84-85 2.2101276077745524 2304 86-87 1.9732594374452894 2304 88-89 2.2576656343243293 2304 90-91 2.267550379474226 2304 92-93 2.0690855686486174 2304 94-95 2.492979287408467 2304 96-97 2.2825711160085227 2304 98-99 2.0712223364674536 2304 100-101 1.749326142490382 1102 1 6.871827677720425 1102 2 7.18163458705752 1102 3 5.893812674401218 1102 4 5.757645601067793 1102 5 6.207751403711043 1102 6 5.3597493469565975 1102 7 6.416583351634298 1102 8 6.241086480505491 1102 9 4.52049514096613 1102 10-11 5.923530337571208 1102 12-13 5.5914699982111316 1102 14-15 5.915271605956331 1102 16-17 5.117180150854509 1102 18-19 5.068138926383 1102 20-21 5.169056440317071 1102 22-23 4.988519329638912 1102 24-25 1.6878229108278724 1102 26-27 5.279228722736967 1102 28-29 4.728190464803337 1102 30-31 4.616497371018433 1102 32-33 4.591056436013044 1102 34-35 4.720264478607607 1102 36-37 4.772552632699231 1102 38-39 3.6805424278667687 1102 40-41 3.5662908027159332 1102 42-43 3.8860554755668737 1102 44-45 -4.185292457252174 1102 46-47 0.9457901160984044 1102 48-49 -1.0753228131517822 1102 50-51 2.3693595985164038 1102 52-53 -0.6098651993683895 1102 54-55 2.2179325876186056 1102 56-57 -1.0801751213675743 1102 58-59 2.6900305223230454 1102 60-61 3.508036631867519 1102 62-63 3.111665792481915 1102 64-65 2.8975954176556264 1102 66-67 2.9717025130758223 1102 68-69 3.2787290374902334 1102 70-71 3.137588931781952 1102 72-73 2.688600035265427 1102 74-75 2.4997911234404846 1102 76-77 2.8132855234626213 1102 78-79 -1.5208993381538036 1102 80-81 1.329237749324271 1102 82-83 2.424677725943692 1102 84-85 2.094267373676896 1102 86-87 2.1508280963821704 1102 88-89 2.0939861922544445 1102 90-91 1.7724897297627145 1102 92-93 1.9309097041639234 1102 94-95 2.267119132025556 1102 96-97 2.1347763096074424 1102 98-99 2.0022288619198676 1102 100-101 1.5920501449777689 2202 1 -20.488142725535216 2202 2 -7.634389578049555 2202 3 -0.6479876327568661 2202 4 1.5526058033010806 2202 5 1.1361713768367991 2202 6 1.5103692166941762 2202 7 3.731105047701451 2202 8 2.322742595398317 2202 9 2.9116410043116794 2202 10-11 2.0294408341434504 2202 12-13 1.293998415344845 2202 14-15 2.552754746919174 2202 16-17 1.537170897696285 2202 18-19 1.6603605946405793 2202 20-21 1.0427386377584185 2202 22-23 1.376622841412889 2202 24-25 2.4374400488794805 2202 26-27 3.515542358033926 2202 28-29 -0.9539099353038942 2202 30-31 1.0811979085868373 2202 32-33 1.422131597880682 2202 34-35 1.3847138948151674 2202 36-37 3.1098299159452516 2202 38-39 2.4925377110944176 2202 40-41 1.7367827483761786 2202 42-43 0.679435458847724 2202 44-45 0.7971316363684089 2202 46-47 -1.4426835220045646 2202 48-49 0.6704901745076128 2202 50-51 1.7030219701476277 2202 52-53 1.54847095250366 2202 54-55 -0.9468163035473829 2202 56-57 0.8432573326651926 2202 58-59 1.7522854869180229 2202 60-61 2.007831442248758 2202 62-63 -2.2661306363668956 2202 64-65 -0.13444915450689976 2202 66-67 0.25543557783373316 2202 68-69 1.2547471563098078 2202 70-71 -2.7069006371194178 2202 72-73 -4.847445024608689 2202 74-75 -1.36744100472745 2202 76-77 -0.3288756401637798 2202 78-79 1.3123215876797971 2202 80-81 1.4455062712992657 2202 82-83 -0.779819659705062 2202 84-85 -0.46498565927954516 2202 86-87 0.2848158317719083 2202 88-89 1.0696301761566254 2202 90-91 1.2355789818185805 2202 92-93 1.349845190109228 2202 94-95 1.488194619590999 2202 96-97 1.1208441265719564 2202 98-99 1.2318296983786468 2202 100-101 0.8880229333435068 2305 1 5.937489507640951 2305 2 7.390025067448011 2305 3 6.778339701331813 2305 4 7.912720711686898 2305 5 8.09510585215849 2305 6 6.151573103608687 2305 7 3.0321808866555493 2305 8 6.287040881157939 2305 9 6.5282611148273055 2305 10-11 4.940930318129713 2305 12-13 5.346316853612549 2305 14-15 5.661279795805552 2305 16-17 5.131397418931016 2305 18-19 4.426002638957129 2305 20-21 5.139033045505158 2305 22-23 5.308453412500604 2305 24-25 5.628852301581173 2305 26-27 6.221225272426402 2305 28-29 5.270492131808563 2305 30-31 5.3967601769147535 2305 32-33 5.507155675812488 2305 34-35 5.3676609910949615 2305 36-37 4.8129842846602955 2305 38-39 4.725852531625048 2305 40-41 4.3890465190470636 2305 42-43 4.086912534740858 2305 44-45 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-25.983534523668563 1209 40-41 -22.52158210805569 1209 42-43 -23.4566726775786 1209 44-45 -26.02047558710789 1209 46-47 -19.607464452520208 1209 48-49 -19.334942070733106 1209 50-51 -21.71063684078312 1209 52-53 -24.126698347722073 1209 54-55 -21.166455129732487 1209 56-57 -24.91091873956957 1209 58-59 -25.16938494197588 1209 60-61 -24.899843501317832 1209 62-63 -25.04649181018179 1209 64-65 -24.925194075500126 1209 66-67 -12.85991739098729 1209 68-69 -24.598074514119087 1209 70-71 -24.427893865110388 1209 72-73 -14.746077525629495 1209 74-75 -16.112474479561616 1209 76-77 -12.978723481826659 1209 78-79 -13.900918884126611 1209 80-81 -23.502800726443397 1209 82-83 -15.11026827641243 1209 84-85 -12.467044226756952 1209 86-87 -12.845572692022573 1209 88-89 -10.132011593040364 1209 90-91 -12.534839126179172 1209 92-93 -16.838634690321285 1209 94-95 -22.193295222395456 1209 96-97 -18.089948714667194 1209 98-99 -9.83311841370234 1209 100-101 -5.224326930677016 1212 1 -20.488142725535216 1212 2 -20.329649171729013 1212 3 -21.210515849461157 1212 4 -23.158003677511562 1212 5 -23.319810564472455 1212 6 -24.354642069987538 1212 7 -23.943387051621347 1212 8 -23.586512483608455 1212 9 -24.49874274286665 1212 10-11 -24.20780521551795 1212 12-13 -24.497875173819942 1212 14-15 -24.814288142471344 1212 16-17 -25.841384407044124 1212 18-19 -20.50815857374912 1212 20-21 -26.21866091077431 1212 22-23 -26.428794765810586 1212 24-25 -22.453283531749157 1212 26-27 -25.121252224358848 1212 28-29 -19.932860748134672 1212 30-31 -15.132617369217286 1212 32-33 -25.286672013857466 1212 34-35 -21.590296538251966 1212 36-37 -25.73373667547687 1212 38-39 -25.983534523668563 1212 40-41 -20.44707397715828 1212 42-43 -17.138725191416896 1212 44-45 -13.558290713158309 1212 46-47 -16.768343650615826 1212 48-49 -19.792753181525544 1212 50-51 -12.120056922809106 1212 52-53 -4.8577907846968635 1212 54-55 -4.250488743177865 1212 56-57 -4.213439747972931 1212 58-59 -2.5223261184464683 1212 60-61 -13.866230055939681 1212 62-63 -11.69144979337507 1212 64-65 -15.877925167937102 1212 66-67 -12.350703807943152 1212 68-69 -8.39639384185018 1212 70-71 -15.084406470152405 1212 72-73 -11.627147018272968 1212 74-75 -8.372817353912719 1212 76-77 -18.594621035387334 1212 78-79 -9.677154292965916 1212 80-81 -7.588935180224908 1212 82-83 -5.444158487787082 1212 84-85 -4.677916971759391 1212 86-87 -3.5639597055310475 1212 88-89 -3.3973197640903585 1212 90-91 -3.9273807844807145 1212 92-93 -3.8364585638980166 1212 94-95 -3.753169171975287 1212 96-97 -12.844261531944275 1212 98-99 -10.040904429132404 1212 100-101 -6.819800444314552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 231.0 3 428.0 4 232.0 5 1408.0 6 1718.0 7 1598.0 8 3403.0 9 6555.0 10 8570.0 11 10854.0 12 14808.0 13 18685.0 14 21576.0 15 23437.0 16 25416.0 17 27777.0 18 31327.0 19 35226.0 20 37422.0 21 41882.0 22 46228.0 23 49605.0 24 51748.0 25 53717.0 26 55974.0 27 58135.0 28 59337.0 29 60158.0 30 62081.0 31 65766.0 32 71088.0 33 80141.0 34 88283.0 35 94879.0 36 97059.0 37 85582.0 38 31506.0 39 101.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 28.959917671797324 28.39504572213421 19.812330593111497 22.83270601295697 2 24.850791365662722 29.855635980439228 23.468131236017214 21.825441417880835 3 21.146244293537855 27.08018998421448 27.488660694981416 24.284905027266255 4 23.662141302196716 26.70761923609737 26.587294676429845 23.042944785276074 5 22.872542040695578 26.989017186069947 26.75797440425014 23.380466368984337 6 22.882935132627946 27.33000490461159 26.073743757346747 23.713316205413715 7 23.42958514945408 27.316910228350704 26.637763737933 22.615740884262216 8 22.850766143984227 27.209441711100247 26.99644039033658 22.943351754578945 9 22.790716954763216 26.41005939585591 27.464513492867166 23.334710156513705 10-11 23.374800325535265 26.777443860306942 26.609106090178653 23.23864972397914 12-13 23.207592787385146 26.903056375350392 26.564388852666116 23.324961984598346 14-15 23.281933075784313 26.943488425592772 26.575334600302273 23.199243898320642 16-17 23.42168799682164 26.995724123672275 26.51309867581466 23.069489203691422 18-19 23.466154619454176 26.926306425541608 26.33998343802095 23.26755551698326 20-21 23.45375732475482 26.927352395270336 26.323646817224688 23.295243462750147 22-23 23.330312169258548 26.942388910686958 26.493630218204302 23.233668701850196 24-25 23.30732283107096 26.931311572161437 26.54070004993871 23.220665546828894 26-27 23.234816670201997 27.03172528904535 26.491937064517774 23.241520976234884 28-29 23.48882880908104 26.983969449025004 26.417225586191655 23.1099761557023 30-31 23.439351850523288 26.943393816953588 26.329099575572002 23.288154756951123 32-33 23.256030053548248 26.993307372737036 26.47536093544923 23.275301638265486 34-35 23.35933431754127 26.938415425222566 26.54602066836666 23.156229588869504 36-37 23.346086508697994 27.00744973984318 26.46142239797068 23.18504135348815 38-39 23.36009364032084 27.004594850849685 26.542192400262564 23.093119108566913 40-41 23.508929382895573 26.90999045714805 26.37331154003454 23.20776861992184 42-43 23.357917481687185 26.997573821270326 26.387464343219936 23.257044353822558 44-45 23.268422386404065 26.931378789833616 26.594082131913915 23.206116691848404 46-47 23.39169386901323 27.048215744253355 26.401584898357616 23.1585054883758 48-49 23.357253862165013 26.977743708822732 26.490484206800453 23.1745182222118 50-51 23.420144998693207 26.92156428580852 26.370536448098946 23.287754267399325 52-53 23.29763577704933 26.955190948724105 26.59233011957039 23.15484315465617 54-55 23.34994040166918 26.936695788551262 26.443554417311155 23.2698093924684 56-57 23.234435959226666 27.023674436230678 26.560850869588975 23.18103873495368 58-59 23.37884226642755 27.01119357239185 26.43914813663799 23.170816024542606 60-61 23.419314378445367 26.80523377730728 26.394412921737008 23.381038922510346 62-63 23.348491932442418 26.89431873879702 26.45915935809842 23.298029970662142 64-65 23.40905748162022 26.96489740931612 26.424357444885526 23.201687664178134 66-67 23.585705243813372 26.899937624499486 26.33548700016203 23.178870131525116 68-69 23.51415201136576 26.931379513332693 26.401050540269765 23.15341793503178 70-71 23.60928382263708 26.98201333794566 26.24543492931229 23.163267910104967 72-73 23.48203504855829 26.979054360828382 26.334070364190705 23.204840226422625 74-75 23.55407106750281 26.90193685622359 26.301084732275477 23.24290734399812 76-77 23.659828406972196 27.02553159525656 26.1996577167315 23.114982281039744 78-79 23.724252034226907 26.911829931657337 26.222208692370945 23.14170934174481 80-81 23.772949705434698 26.92946170580649 26.11887460668587 23.17871398207294 82-83 23.715039458675033 26.974053985013924 26.19130197926704 23.119604577044004 84-85 23.897017229615066 26.81292526806425 26.050069111001335 23.23998839131935 86-87 23.790481445619683 26.94236254013218 26.158188019829296 23.108967994418837 88-89 23.95250697274471 26.872608472433896 26.15781648459811 23.017068070223278 90-91 24.072870117474405 26.826813459302855 25.89276050093549 23.20755592228724 92-93 24.00809441799566 26.854258625376787 25.968360166432152 23.1692867901954 94-95 24.140579297738217 26.82507560955743 25.884156672664655 23.150188420039697 96-97 24.227300691534083 26.858872220879356 25.833586442464828 23.080240645121737 98-99 24.203908489663434 26.839024181140182 25.84635396079002 23.11071336840637 100-101 24.599633530386118 26.78796456639297 25.500675825709106 23.111726077511808 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 91.0 1 87.0 2 101.0 3 102.5 4 101.5 5 120.0 6 141.5 7 187.5 8 240.0 9 288.0 10 388.0 11 530.5 12 694.0 13 910.5 14 1165.0 15 1559.5 16 2060.5 17 2603.5 18 3238.5 19 4129.5 20 5273.0 21 6646.0 22 8345.5 23 10150.0 24 12203.0 25 14531.0 26 16943.5 27 19332.0 28 21734.0 29 24451.0 30 26912.0 31 29061.0 32 31352.5 33 33468.5 34 35230.0 35 36644.5 36 38179.5 37 39076.5 38 39019.0 39 38420.5 40 37535.0 41 36551.5 42 35162.0 43 33736.5 44 32576.0 45 31777.5 46 31292.5 47 31006.5 48 30694.0 49 30439.5 50 30208.5 51 30174.0 52 30279.5 53 30308.5 54 30662.0 55 31544.0 56 32122.0 57 32154.5 58 32333.0 59 32311.5 60 31454.5 61 30123.0 62 28458.5 63 26440.5 64 24192.0 65 21514.5 66 18847.0 67 16410.0 68 13984.5 69 11639.5 70 9634.0 71 7960.5 72 6507.5 73 5366.0 74 4490.0 75 3683.5 76 2951.0 77 2350.5 78 1895.5 79 1569.5 80 1274.5 81 970.5 82 764.5 83 625.5 84 502.5 85 408.5 86 329.5 87 266.0 88 211.0 89 166.5 90 120.5 91 86.5 92 59.5 93 43.5 94 35.5 95 25.0 96 16.5 97 13.0 98 8.0 99 4.0 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.333136695972655 2 10.63204163655657 3 9.376652543890513 4 11.284947901633565 5 8.842571426765575 6 5.0670638741352345 7 9.385571452749799 8 9.660793530069013 9 5.126968041512956 10-11 6.071283852350624 12-13 5.1618360592187456 14-15 7.044498332444954 16-17 2.5144651358448136 18-19 2.897311054320369 20-21 2.9304233813058267 22-23 2.9899412967250747 24-25 3.3185012581279705 26-27 4.15371844760422 28-29 1.6430807175297293 30-31 2.1113936602710366 32-33 2.88467008113398 34-35 1.8876133210575439 36-37 2.908090995343206 38-39 1.3036705874751833 40-41 1.0925312214480798 42-43 0.5133639666250217 44-45 1.205632817651855 46-47 1.2939440608845452 48-49 1.1932025273519058 50-51 1.520603732879382 52-53 2.3500271429785364 54-55 1.6667825422542086 56-57 1.6893607249176756 58-59 1.4742886116770288 60-61 1.2880098262498236 62-63 0.563436265968885 64-65 1.1624077121172858 66-67 0.5280766548614022 68-69 0.7425167194427297 70-71 0.9290413015707815 72-73 0.0783038061267988 74-75 0.09677367250468945 76-77 0.4290556982346881 78-79 0.8024560006348578 80-81 0.26668942041840216 82-83 0.4793386804649912 84-85 0.06095758180992049 86-87 0.09119057601403428 88-89 0.07581072530392763 90-91 0.08167473230983587 92-93 0.15594044977987148 94-95 0.41894291968557684 96-97 0.34566038901892704 98-99 0.06394225603448458 100-101 0.21675757633216544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1423941.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.28450804019153 #Duplication Level Percentage of deduplicated Percentage of total 1 99.39389250717272 98.68273719774322 2 0.5176222841633059 1.0278374766758807 3 0.06752245618117284 0.20111801530839396 4 0.015870067153103098 0.06302607239442576 5 0.00509268532969037 0.025281237878090442 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.8091051525308986E-4 0.0 0.0 2 4.2136577287963474E-4 7.022762881327247E-5 0.0 3 4.2136577287963474E-4 7.022762881327247E-5 0.0 4 4.915934016929073E-4 7.022762881327247E-5 0.0 5 6.320486593194522E-4 7.022762881327247E-5 0.0 6 7.022762881327247E-4 7.022762881327247E-5 0.0 7 7.72503916945997E-4 7.022762881327247E-5 0.0 8 9.129591745725419E-4 7.022762881327247E-5 0.0 9 9.831868033858145E-4 7.022762881327247E-5 0.0 10-11 0.0012992111330455405 7.022762881327247E-5 7.022762881327247E-5 12-13 0.001685463091518539 7.022762881327247E-5 7.022762881327247E-5 14-15 0.0021770564932114466 7.022762881327247E-5 7.022762881327247E-5 16-17 0.002738877523717626 7.022762881327247E-5 7.022762881327247E-5 18-19 0.0030197880389707154 1.4045525762654493E-4 7.022762881327247E-5 20-21 0.003406039997443714 1.4045525762654493E-4 7.022762881327247E-5 22-23 0.003511381440663623 2.1068288643981737E-4 1.053414432199087E-4 24-25 0.0037220643271034404 2.1068288643981737E-4 1.4045525762654493E-4 26-27 0.004389226800829529 2.1068288643981737E-4 1.4045525762654493E-4 28-29 0.004810592573709163 2.1068288643981737E-4 1.4045525762654493E-4 30-31 0.005477755047435252 2.4579670084645363E-4 1.4045525762654493E-4 32-33 0.006636510922854248 2.8091051525308986E-4 1.4045525762654493E-4 34-35 0.008321974014372787 2.8091051525308986E-4 1.7556907203318116E-4 36-37 0.010674599579617415 2.8091051525308986E-4 2.1068288643981737E-4 38-39 0.01397529813384122 2.8091051525308986E-4 2.1068288643981737E-4 40-41 0.018469866377890658 2.8091051525308986E-4 2.1068288643981737E-4 42-43 0.02486058059989845 3.160243296597261E-4 2.1068288643981737E-4 44-45 0.03258561976935842 3.511381440663623E-4 2.1068288643981737E-4 46-47 0.04175032532949048 3.8625195847299856E-4 3.511381440663623E-4 48-49 0.05505846098960561 4.2136577287963474E-4 3.511381440663623E-4 50-51 0.07243979912089055 4.2136577287963474E-4 3.511381440663623E-4 52-53 0.08890817807760293 4.2136577287963474E-4 3.511381440663623E-4 54-55 0.11131079166903685 4.2136577287963474E-4 3.8625195847299856E-4 56-57 0.1369438761858813 4.2136577287963474E-4 4.2136577287963474E-4 58-59 0.1714256419331981 4.2136577287963474E-4 4.2136577287963474E-4 60-61 0.21201721138726956 4.2136577287963474E-4 4.2136577287963474E-4 62-63 0.25408356104641977 4.2136577287963474E-4 4.2136577287963474E-4 64-65 0.30046890987758623 4.2136577287963474E-4 4.2136577287963474E-4 66-67 0.35696703725786394 4.2136577287963474E-4 4.2136577287963474E-4 68-69 0.4209092932923485 4.2136577287963474E-4 4.2136577287963474E-4 70-71 0.4992833270479605 4.2136577287963474E-4 4.2136577287963474E-4 72-73 0.5733032478171498 4.915934016929073E-4 4.2136577287963474E-4 74-75 0.6542054762100395 4.915934016929073E-4 4.2136577287963474E-4 76-77 0.7438861582045886 4.915934016929073E-4 4.2136577287963474E-4 78-79 0.8441360983355349 4.915934016929073E-4 4.2136577287963474E-4 80-81 0.9631368153596251 4.915934016929073E-4 4.2136577287963474E-4 82-83 1.082558898156595 5.618210305061797E-4 4.915934016929073E-4 84-85 1.2058083867238882 5.618210305061797E-4 4.915934016929073E-4 86-87 1.3391706538402925 5.618210305061797E-4 4.915934016929073E-4 88-89 1.4943737135176245 5.618210305061797E-4 4.915934016929073E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAGG 1260 0.0 6.131822 94-95 TCGTGTA 1225 1.4551915E-11 5.9361215 92-93 CGTGTAG 1200 1.8062565E-9 5.4915876 94-95 GTCGTGT 1465 1.0913936E-11 5.428993 92-93 >>END_MODULE