FastQCFastQC Report
Thu 11 Sep 2014
NYCLib1_CGATGT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib1_CGATGT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3911761
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG8400.0078318726.7660471
TACCTCT14652.6545674E-45.8192621
GGTACAA13750.00467686775.37347131
GTAGGGA138100.05.29310794-95
CGAACTC15250.00275855335.21762041
GAGTGTA23650.05.16730994-95
TGTAGGG209450.05.132335794-95
GAACTCC25959.1030597E-75.0552082
GGCATAA15750.00412518345.05198141
AGAGGGG47400.05.013173694-95
TACCACC15900.0046406095.0043211
Produced by FastQC (version 0.11.2)