##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib1_CGATGT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3911761 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.258711357876926 33.0 33.0 33.0 27.0 33.0 2 31.644688159629386 33.0 33.0 33.0 27.0 33.0 3 31.783172591577042 33.0 33.0 33.0 27.0 33.0 4 35.63191181669841 37.0 37.0 37.0 33.0 37.0 5 35.16419714803639 37.0 37.0 37.0 33.0 37.0 6 34.943023870834644 37.0 33.0 37.0 33.0 37.0 7 34.730367729521305 37.0 33.0 37.0 27.0 37.0 8 34.764952664541624 37.0 33.0 37.0 27.0 37.0 9 34.68570651427835 37.0 33.0 37.0 27.0 37.0 10-11 34.5286918091366 37.0 33.0 37.0 27.0 37.0 12-13 34.52297341274173 37.0 33.0 37.0 27.0 37.0 14-15 35.43398957656156 37.0 33.0 40.0 27.0 40.0 16-17 35.33412624135268 37.0 33.0 40.0 27.0 40.0 18-19 35.55429434466983 37.0 33.0 40.0 27.0 40.0 20-21 35.242979696356706 37.0 33.0 40.0 27.0 40.0 22-23 35.27132089613859 37.0 33.0 40.0 27.0 40.0 24-25 35.12490320855492 37.0 33.0 40.0 27.0 40.0 26-27 34.74668109835954 37.0 33.0 40.0 27.0 40.0 28-29 34.39146486710205 37.0 33.0 40.0 27.0 40.0 30-31 34.29727608614125 37.0 33.0 40.0 27.0 40.0 32-33 34.327107918914265 37.0 33.0 40.0 27.0 40.0 34-35 34.19118448187402 37.0 33.0 38.5 24.5 40.0 36-37 33.91435711435336 37.0 33.0 37.0 22.0 40.0 38-39 33.84134715796799 37.0 33.0 37.0 22.0 40.0 40-41 33.5741954838243 37.0 33.0 37.0 22.0 40.0 42-43 33.20878320531341 37.0 33.0 37.0 22.0 40.0 44-45 33.1216452129872 37.0 33.0 37.0 22.0 40.0 46-47 32.98549489603276 37.0 33.0 37.0 22.0 40.0 48-49 33.01831502486988 37.0 33.0 37.0 22.0 40.0 50-51 33.30378709230958 37.0 33.0 37.0 22.0 40.0 52-53 33.152991197570614 37.0 33.0 37.0 22.0 40.0 54-55 32.79520233981575 37.0 33.0 37.0 22.0 40.0 56-57 32.838375095001965 37.0 33.0 37.0 22.0 40.0 58-59 32.540544399312736 37.0 33.0 37.0 22.0 40.0 60-61 32.459539194751414 37.0 33.0 37.0 22.0 40.0 62-63 32.44154039574504 37.0 33.0 37.0 22.0 40.0 64-65 32.09401417929163 33.0 27.0 37.0 22.0 40.0 66-67 31.742762019458752 33.0 27.0 37.0 18.5 40.0 68-69 31.57301800902458 33.0 27.0 37.0 22.0 40.0 70-71 31.422122670582382 33.0 27.0 37.0 18.5 38.5 72-73 31.183420203841695 33.0 27.0 37.0 15.0 37.0 74-75 30.82769154352733 33.0 27.0 37.0 15.0 37.0 76-77 29.717630371589674 33.0 27.0 37.0 15.0 37.0 78-79 30.63026728882465 33.0 27.0 37.0 15.0 37.0 80-81 30.33776283878284 33.0 27.0 37.0 15.0 37.0 82-83 30.318809482481164 33.0 27.0 37.0 15.0 37.0 84-85 30.18714729759819 33.0 27.0 37.0 15.0 37.0 86-87 29.99009129136468 33.0 27.0 37.0 15.0 37.0 88-89 29.85183604008527 33.0 27.0 37.0 15.0 37.0 90-91 29.765990815901073 33.0 27.0 37.0 15.0 37.0 92-93 29.550392904883502 33.0 27.0 33.0 15.0 37.0 94-95 29.450036697027247 33.0 27.0 33.0 15.0 37.0 96-97 29.301127420616954 33.0 27.0 33.0 15.0 37.0 98-99 29.035199236354163 33.0 27.0 33.0 15.0 37.0 100-101 28.188533501918954 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.080540567149118 2306 2 0.554366560055982 2306 3 0.5110352625572929 2306 4 0.35703699619993756 2306 5 0.615107315209336 2306 6 0.6701724802453768 2306 7 0.8517183425231067 2306 8 0.8580897321232115 2306 9 1.041544279402551 2306 10-11 1.1807067905147548 2306 12-13 1.384454181257233 2306 14-15 1.4956985026199376 2306 16-17 1.4758827593387096 2306 18-19 1.2729920128151235 2306 20-21 1.5540941574159106 2306 22-23 1.2157990873337283 2306 24-25 1.1781969767539735 2306 26-27 1.2888289328835896 2306 28-29 1.5649535235299652 2306 30-31 1.584698295634567 2306 32-33 1.284158797844725 2306 34-35 1.1812965688682553 2306 36-37 1.3055082484680085 2306 38-39 1.0943232549459125 2306 40-41 1.1655219306566664 2306 42-43 1.0921432514100076 2306 44-45 0.965947624496863 2306 46-47 0.8766682044057461 2306 48-49 0.9872264357157903 2306 50-51 1.0327726279349534 2306 52-53 1.094190526301503 2306 54-55 1.5040418272850218 2306 56-57 1.1638408165333516 2306 58-59 1.0647188363673692 2306 60-61 0.8900242678907588 2306 62-63 0.5897399523457381 2306 64-65 0.6886690773751845 2306 66-67 0.8410025289065786 2306 68-69 0.7107168787690874 2306 70-71 0.6313322673896948 2306 72-73 0.4103508348314868 2306 74-75 0.6741711481302204 2306 76-77 0.7507386498807698 2306 78-79 0.7031238585986586 2306 80-81 1.249076605295361 2306 82-83 1.076801840602812 2306 84-85 0.9022688854684553 2306 86-87 0.7148844933625291 2306 88-89 0.5716119550557899 2306 90-91 0.1908370684448002 2306 92-93 -0.03472811730411962 2306 94-95 -0.12098859079738844 2306 96-97 -0.11288240648414316 2306 98-99 0.19355165188464696 2306 100-101 0.06330956280723754 2307 1 0.6719061662332493 2307 2 0.4868990455563207 2307 3 0.3200960651158695 2307 4 0.3476499090764804 2307 5 0.49944904361303344 2307 6 0.6165060321073881 2307 7 0.7966477826969793 2307 8 0.7869756993824737 2307 9 0.9037229693497864 2307 10-11 1.0804512225572793 2307 12-13 1.3005089618223664 2307 14-15 1.2950220370124939 2307 16-17 1.3009786601149003 2307 18-19 1.1169619761862108 2307 20-21 1.3407427797109364 2307 22-23 1.1273518131955456 2307 24-25 1.1284975219735287 2307 26-27 1.2503823348013228 2307 28-29 1.4861382366866778 2307 30-31 1.5751719511250997 2307 32-33 1.26546409274075 2307 34-35 1.2602496364871953 2307 36-37 1.3156086324938272 2307 38-39 0.9393658092641104 2307 40-41 1.1491025807838824 2307 42-43 1.1870999886083595 2307 44-45 1.0358453718351086 2307 46-47 0.9314836797919099 2307 48-49 0.9752669702915924 2307 50-51 0.9118598745583881 2307 52-53 1.250997231027469 2307 54-55 1.3966860869781712 2307 56-57 1.0721365525198223 2307 58-59 1.0406188325542374 2307 60-61 0.9155806800090076 2307 62-63 0.715370374491556 2307 64-65 0.8763238156455664 2307 66-67 0.9756246323218178 2307 68-69 0.8436358858511106 2307 70-71 0.786289553829203 2307 72-73 0.6353385463931964 2307 74-75 0.8209375610814469 2307 76-77 0.8468345992113804 2307 78-79 0.917974864414969 2307 80-81 1.3760066110399514 2307 82-83 1.1017329444243629 2307 84-85 0.9245633350088163 2307 86-87 0.9307458915336149 2307 88-89 0.8656632955820278 2307 90-91 0.6328540539227419 2307 92-93 0.6838326259439427 2307 94-95 0.5628557833880699 2307 96-97 0.5700372259858 2307 98-99 0.6249943772232776 2307 100-101 0.6565883132868109 1101 1 1.0526644835492611 1101 2 0.5447249629196165 1101 3 0.30688212431104844 1101 4 0.3164684224219485 1101 5 0.5276413972113119 1101 6 0.5908332319992482 1101 7 0.7362022241720254 1101 8 0.6355934812293 1101 9 0.860713368953121 1101 10-11 0.9356873797741656 1101 12-13 1.2131488855203756 1101 14-15 1.268813167103282 1101 16-17 1.310474305422204 1101 18-19 0.9624141094934018 1101 20-21 1.3174708923721425 1101 22-23 0.9826202312158188 1101 24-25 1.052859849165749 1101 26-27 1.1015764116684252 1101 28-29 1.3474828437264108 1101 30-31 1.3364408800298122 1101 32-33 0.8122782813805998 1101 34-35 0.9222539184786669 1101 36-37 1.121941174675932 1101 38-39 0.8779547802739316 1101 40-41 0.9553270154955698 1101 42-43 1.0899538398610815 1101 44-45 0.8896533728514129 1101 46-47 0.7727541926069108 1101 48-49 0.7412057176826963 1101 50-51 0.7562627726347628 1101 52-53 1.06962193111314 1101 54-55 1.3537807294386823 1101 56-57 1.0212674184361106 1101 58-59 0.9848563934683838 1101 60-61 0.7982020678015047 1101 62-63 0.5988432702445152 1101 64-65 0.6805208684392063 1101 66-67 0.6927450331560223 1101 68-69 0.5059311325395477 1101 70-71 0.7513272622844092 1101 72-73 0.680724956576622 1101 74-75 0.7627428491973731 1101 76-77 0.7698454232085226 1101 78-79 0.7626221121071559 1101 80-81 1.3532461820179051 1101 82-83 1.0708595589755063 1101 84-85 0.948397559895831 1101 86-87 0.6949604475383673 1101 88-89 0.6526655363022194 1101 90-91 0.42222579653322256 1101 92-93 0.6555544590444882 1101 94-95 0.6819006395762237 1101 96-97 0.5642672785085097 1101 98-99 0.610413235048167 1101 100-101 0.621481846969445 2201 1 1.4297704132765858 2201 2 0.8100458527247341 2201 3 0.34365630834279415 2201 4 0.22239817582689625 2201 5 0.4465504746947815 2201 6 0.5016370802588241 2201 7 0.548017666963112 2201 8 0.6063370303207023 2201 9 0.5961331198113058 2201 10-11 0.8160047302576032 2201 12-13 1.0139598721220082 2201 14-15 1.0045224349386714 2201 16-17 0.9784466638791898 2201 18-19 0.8757825314642389 2201 20-21 1.1390310617680797 2201 22-23 0.9037575338290154 2201 24-25 0.7902743626508766 2201 26-27 0.989595431037813 2201 28-29 1.2696415447892662 2201 30-31 1.3601169583999564 2201 32-33 0.9440176781663538 2201 34-35 0.8363421372814841 2201 36-37 0.9750144856691989 2201 38-39 0.6545779967172152 2201 40-41 0.9641990905054314 2201 42-43 0.9892973627777906 2201 44-45 0.8755787804225861 2201 46-47 0.8559188915927152 2201 48-49 0.8811688137409206 2201 50-51 0.7698543272040723 2201 52-53 0.9978537798382519 2201 54-55 1.3807991889733415 2201 56-57 0.9934940601011846 2201 58-59 1.0793811544040608 2201 60-61 0.8077773123952028 2201 62-63 0.7312290214159134 2201 64-65 0.8554109808037573 2201 66-67 0.7097417539583972 2201 68-69 0.7822059230057938 2201 70-71 0.9056724860345646 2201 72-73 0.8331520769209781 2201 74-75 0.7942950919965206 2201 76-77 0.7828446927398218 2201 78-79 0.7683324307556525 2201 80-81 1.3386432343277654 2201 82-83 1.1192050688157664 2201 84-85 1.0641133175019384 2201 86-87 1.025341259772265 2201 88-89 1.0203768824639319 2201 90-91 0.8905322981973107 2201 92-93 0.9131195236761585 2201 94-95 0.7966763309712483 2201 96-97 0.6108918572993787 2201 98-99 0.7988177790816415 2201 100-101 0.9052517806853366 2304 1 -1.7492073978677283 2304 2 -0.19396273532164798 2304 3 -0.08142392644624863 2304 4 0.01636338189648967 2304 5 0.42893713123748967 2304 6 0.5443150056100805 2304 7 0.5877032366928674 2304 8 0.7129134056516975 2304 9 0.9202988221478279 2304 10-11 0.89672327377545 2304 12-13 1.2303182052553012 2304 14-15 1.2735545686442151 2304 16-17 1.193228115812353 2304 18-19 0.9573865103687851 2304 20-21 1.17631021810773 2304 22-23 1.0073145336601002 2304 24-25 0.9941755453104051 2304 26-27 1.0646326751724402 2304 28-29 1.5032615793111361 2304 30-31 1.610242055504358 2304 32-33 1.0249489278669728 2304 34-35 0.8987464921185904 2304 36-37 1.2441326390485017 2304 38-39 1.0634995495150932 2304 40-41 0.9339748901869598 2304 42-43 0.849323677837468 2304 44-45 1.0162636417163355 2304 46-47 0.8753699704312368 2304 48-49 0.7763756955778902 2304 50-51 0.89643689463621 2304 52-53 1.0789863906611288 2304 54-55 1.3927936034766901 2304 56-57 0.9903163196141307 2304 58-59 0.8471776630411583 2304 60-61 0.4130636940586001 2304 62-63 0.24180198752882376 2304 64-65 0.3936399708204341 2304 66-67 0.3689650744044002 2304 68-69 0.45697526477195893 2304 70-71 0.5241918668507601 2304 72-73 0.31073330563597423 2304 74-75 0.6045541068528486 2304 76-77 0.572787937841472 2304 78-79 0.547151350765084 2304 80-81 1.2289008472196095 2304 82-83 0.8880253184443667 2304 84-85 0.521228742147553 2304 86-87 0.3808742457823264 2304 88-89 0.35687567950387233 2304 90-91 0.2570829273658646 2304 92-93 0.3228647443090864 2304 94-95 0.051482389080103275 2304 96-97 0.10464314046956957 2304 98-99 0.4396158991557897 2304 100-101 0.4292611623378839 1102 1 1.4538617399057472 1102 2 0.0405313824953879 1102 3 0.2920313006959532 1102 4 0.15259464301099257 1102 5 0.20789889072718637 1102 6 0.12232102324398397 1102 7 -4.705321581531969 1102 8 -1.185776392424053 1102 9 0.22788323216565942 1102 10-11 0.6144960027447297 1102 12-13 0.8724645789626422 1102 14-15 0.725969568662272 1102 16-17 0.421726452474779 1102 18-19 0.8242494304021619 1102 20-21 1.0550922191455925 1102 22-23 0.7032809249700449 1102 24-25 0.9536367033979047 1102 26-27 0.995627643621404 1102 28-29 0.8649420258537646 1102 30-31 1.0805055768195189 1102 32-33 0.9066858062825887 1102 34-35 1.1499447212630614 1102 36-37 1.2422412622769556 1102 38-39 0.9170346385334582 1102 40-41 1.135636060555342 1102 42-43 1.1605205344123775 1102 44-45 1.1288836362019765 1102 46-47 0.9635456448866364 1102 48-49 0.8829787183613433 1102 50-51 0.7254361589870797 1102 52-53 0.8832462927270868 1102 54-55 1.3216950421463025 1102 56-57 1.116728049545415 1102 58-59 1.0259310688798777 1102 60-61 0.8698747092905137 1102 62-63 0.6299817556127252 1102 64-65 0.43211562396487935 1102 66-67 0.5529144386277736 1102 68-69 0.7461483397732529 1102 70-71 0.605529134883497 1102 72-73 0.5664040045267207 1102 74-75 0.5757500724615561 1102 76-77 0.6509835265955495 1102 78-79 0.8020866413344478 1102 80-81 1.3494181735022686 1102 82-83 1.0636785832937008 1102 84-85 0.7775261876029624 1102 86-87 0.7165187334105418 1102 88-89 0.613035386400071 1102 90-91 0.06652175109783443 1102 92-93 0.18855580298161811 1102 94-95 0.31377338827462253 1102 96-97 0.06807644261908763 1102 98-99 0.1859745563974755 1102 100-101 0.4006569331380341 2202 1 1.5162212157998738 2202 2 0.8778629210329925 2202 3 0.4108959010083453 2202 4 -0.21185223986051227 2202 5 0.12646864693055448 2202 6 0.46970150398980337 2202 7 0.6853518602759294 2202 8 -3.9956495764958824 2202 9 -0.44717888924461846 2202 10-11 0.4709659861683875 2202 12-13 0.034850849200616096 2202 14-15 0.18995839584619745 2202 16-17 0.35147673344324204 2202 18-19 0.7761697448741884 2202 20-21 1.186180356587812 2202 22-23 -2.6412779093484033 2202 24-25 -2.8279165072981414 2202 26-27 -1.4886100519799612 2202 28-29 0.558311276367057 2202 30-31 1.1518556183401145 2202 32-33 1.0706123791477893 2202 34-35 -1.9925387210020418 2202 36-37 -0.23305871209829832 2202 38-39 0.2812678046147923 2202 40-41 1.1881293167805111 2202 42-43 1.204627745812303 2202 44-45 1.3497999176999542 2202 46-47 0.7080956009205153 2202 48-49 0.9944666869566774 2202 50-51 -3.833144304321145 2202 52-53 -0.3103611828948445 2202 54-55 1.4939396638729008 2202 56-57 1.4502275184432492 2202 58-59 1.5238436619025038 2202 60-61 1.4081556394795527 2202 62-63 1.2864133550755597 2202 64-65 1.2075997031272756 2202 66-67 1.2307308484621515 2202 68-69 1.2105460996810429 2202 70-71 1.3197481063422067 2202 72-73 1.1102283867416567 2202 74-75 0.6449797988721677 2202 76-77 1.197770438196546 2202 78-79 1.177854825602978 2202 80-81 1.850597975245389 2202 82-83 1.633351479645313 2202 84-85 1.3452585673979023 2202 86-87 1.5464444535351127 2202 88-89 1.3683148822410978 2202 90-91 1.0803426351623244 2202 92-93 1.2111738442938176 2202 94-95 1.1963355336084156 2202 96-97 1.1833792027554821 2202 98-99 1.2949522564858356 2202 100-101 1.1867807591576813 2305 1 1.2429773897870398 2305 2 0.6674830145532233 2305 3 0.04626145172903051 2305 4 -0.1387269462199825 2305 5 0.2226203997601175 2305 6 0.23001715588753058 2305 7 0.3595755998795198 2305 8 -0.13364156818815331 2305 9 0.4604562817170432 2305 10-11 0.27102679822215947 2305 12-13 0.7532251743848093 2305 14-15 0.6382087055845602 2305 16-17 0.7101695216259643 2305 18-19 0.6383458246656915 2305 20-21 0.7738277922253189 2305 22-23 0.4968480496097669 2305 24-25 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-0.5663971212713363 1208 42-43 0.3105751516242776 1208 44-45 0.09010055257405014 1208 46-47 -0.11034845009493921 1208 48-49 -1.3378543811654282 1208 50-51 -0.160854359689381 1208 52-53 -1.7283327432417117 1208 54-55 -1.1182677358496917 1208 56-57 -6.286439561699744 1208 58-59 -2.1654089232642235 1208 60-61 -1.5092832130108889 1208 62-63 0.19564417635230313 1208 64-65 -0.10250719929606689 1208 66-67 0.6465418070882052 1208 68-69 0.23061872115851045 1208 70-71 -4.502665317354502 1208 72-73 -0.7702213251405254 1208 74-75 -5.866111214783832 1208 76-77 -1.5342900270770805 1208 78-79 -4.0737744214566405 1208 80-81 0.8077820887333651 1208 82-83 0.1823653386198174 1208 84-85 -2.9629582595848127 1208 86-87 -1.0010859651660446 1208 88-89 -5.097536785008192 1208 90-91 -0.15711403439867766 1208 92-93 -2.3545238969978044 1208 94-95 -4.948429630635655 1208 96-97 -3.627354229060394 1208 98-99 -6.5400194955636906 1208 100-101 -3.9528206120801563 1209 1 1.831834435304863 1209 2 1.2493798156420723 1209 3 0.48022370949659177 1209 4 0.5373205835918711 1209 5 0.5337145347569887 1209 6 1.0818608442182551 1209 7 0.8125679839721514 1209 8 0.1768693042123317 1209 9 -0.4018484107388076 1209 10-11 -3.098676515988238 1209 12-13 -0.24876228276281154 1209 14-15 -1.1283626847084491 1209 16-17 -0.35890742810011744 1209 18-19 -16.746838730243034 1209 20-21 -13.968016258107134 1209 22-23 -3.1627596961445796 1209 24-25 -1.714824758272492 1209 26-27 -1.2870646698884158 1209 28-29 -2.7150708005929154 1209 30-31 -0.6012633232824243 1209 32-33 -0.4359716580135995 1209 34-35 -2.913284064429405 1209 36-37 -3.9623255880136092 1209 38-39 -1.3450325067048752 1209 40-41 -10.81091410930432 1209 42-43 -5.225066351150915 1209 44-45 -3.1029540850466653 1209 46-47 -2.726354738394214 1209 48-49 -2.712141332944441 1209 50-51 -2.165251356705653 1209 52-53 -1.0563670080561138 1209 54-55 -2.4286228964259067 1209 56-57 -0.9284797298154999 1209 58-59 -1.2637620946845445 1209 60-61 -1.3491414054456818 1209 62-63 -0.4816419091813984 1209 64-65 -0.14845926705569923 1209 66-67 0.504979678049736 1209 68-69 -1.0021179723510691 1209 70-71 -0.9255839223787241 1209 72-73 -0.389325242494305 1209 74-75 0.7446299062017658 1209 76-77 -16.285321821488196 1209 78-79 -16.46502758966865 1209 80-81 -21.947948926056974 1209 82-83 -9.578196437503898 1209 84-85 -3.195838364817572 1209 86-87 -2.055912876520278 1209 88-89 -1.825241876605613 1209 90-91 -5.401084967056125 1209 92-93 -2.9473417609010433 1209 94-95 -0.4315882176933066 1209 96-97 0.34891482031559207 1209 98-99 1.3592958498115202 1209 100-101 0.6869612224349062 1212 1 -28.632858047610853 1212 2 -16.27990067736387 1212 3 -5.829184465171394 1212 4 -1.4462828031790878 1212 5 -0.4162443042722046 1212 6 -2.2578097357712323 1212 7 -0.30304640550651385 1212 8 -1.4740096230236688 1212 9 0.059144543957678763 1212 10-11 0.676885438711551 1212 12-13 0.2512120949461192 1212 14-15 -0.1402993662415568 1212 16-17 -0.08276029333347168 1212 18-19 -0.9882883237577857 1212 20-21 -0.7529964710862345 1212 22-23 -0.5642940581164737 1212 24-25 0.16318255482529054 1212 26-27 -0.7728914103031954 1212 28-29 0.17433512592565847 1212 30-31 -0.7360150846083116 1212 32-33 0.13290767256684433 1212 34-35 -1.3206462578297646 1212 36-37 -1.3348621287067672 1212 38-39 -0.4058788357744376 1212 40-41 -0.21839532237929404 1212 42-43 0.28902094978041504 1212 44-45 1.2904456127756063 1212 46-47 -2.097312615347704 1212 48-49 -1.0170771780337233 1212 50-51 0.20495851465391723 1212 52-53 -0.6283664724675759 1212 54-55 -0.25834541527895283 1212 56-57 -1.2532009427582267 1212 58-59 -0.2583599238979204 1212 60-61 0.31655051212658236 1212 62-63 -4.402621937018779 1212 64-65 -3.6733442973561274 1212 66-67 -1.462349421268879 1212 68-69 0.2951402772001295 1212 70-71 -0.5224725497182057 1212 72-73 -1.0717319856200938 1212 74-75 -0.1773105442877032 1212 76-77 0.863987533788837 1212 78-79 -1.2184006403082144 1212 80-81 0.7398814775189102 1212 82-83 0.9119141846714527 1212 84-85 -3.416501668076396 1212 86-87 -1.5418809527986639 1212 88-89 -0.909125124917022 1212 90-91 0.18575724261025783 1212 92-93 -2.2128159716518283 1212 94-95 -1.9906079253677618 1212 96-97 -0.016480539900761926 1212 98-99 -0.8063473027551709 1212 100-101 0.07074098975144594 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 7.0 10 5.0 11 15.0 12 62.0 13 238.0 14 918.0 15 2184.0 16 4247.0 17 7272.0 18 11146.0 19 16322.0 20 23531.0 21 32625.0 22 43549.0 23 56780.0 24 72299.0 25 90465.0 26 112097.0 27 136376.0 28 164505.0 29 195757.0 30 229879.0 31 268208.0 32 309664.0 33 350270.0 34 390354.0 35 424802.0 36 440827.0 37 394171.0 38 132911.0 39 271.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.444602707220387 23.862130120839296 27.605285084128166 22.08798208781215 2 28.332143726339943 33.651732156406396 18.772509648087667 19.243614469166 3 16.292647835518974 27.170066768138827 30.71293483779366 25.824350558548538 4 19.826543595071374 28.47109013050644 30.30338509944754 21.398981174974647 5 20.428983263548055 26.732972694395187 30.134612007226412 22.70343203483035 6 20.857946075948913 29.212802111376433 27.705961586098947 22.223290226575703 7 21.114301206029715 29.334537565050628 28.672457238568512 20.87870399035115 8 20.637636092798104 28.768245299239908 28.85912508458467 21.734993523377323 9 20.325065871866915 27.287914573513056 30.297914417573057 22.08910513704697 10-11 21.29030889157083 27.801877466440306 28.75672874697611 22.151084895012758 12-13 21.560123100882016 28.138246439974235 28.153550804727836 22.148079654415916 14-15 21.75666173836563 28.141919731537758 27.821884800973884 22.27953372912273 16-17 21.933000779447138 28.05457510416701 27.79604611443429 22.216378001951558 18-19 21.959236300622237 28.19620156786609 27.787228629948334 22.057333501563335 20-21 21.98948248627664 28.006900217063365 27.79999851729183 22.203618779368167 22-23 21.852728221381625 28.01753481360441 27.985822753486218 22.14391421152775 24-25 21.69014160118678 28.053631088402387 28.226980124808236 22.029247185602596 26-27 21.957578696653503 28.059255153880823 27.86371662276913 22.119449526696545 28-29 21.91063053187554 28.077252163411824 28.111380015292347 21.900737289420288 30-31 21.978988491372554 27.9696791291697 27.957996411334946 22.093335968122794 32-33 21.966487727650026 27.983764856799787 27.846397057488943 22.20335035806124 34-35 22.086766037086623 27.897665527111705 27.929352023295902 22.086216412505774 36-37 22.021731389008682 28.015451352983987 27.972414470106944 21.990402787900383 38-39 21.954217039333436 27.879221148735827 28.14451087374714 22.022050938183597 40-41 22.047544929115666 27.753186954128132 28.007373795900726 22.191894320855475 42-43 21.705352141912556 28.195434230261 28.184927453389918 21.914286174436526 44-45 21.909582410581834 27.718219492448544 28.229766593613466 22.14243150335616 46-47 21.94403954505745 27.935389862813793 28.09153520417093 22.029035387957823 48-49 21.937235413215532 27.873910976133505 28.265729492606905 21.92312411804405 50-51 22.169513597086933 27.817474347205106 27.924548994224335 22.088463061483623 52-53 21.90260868809413 28.045988559356534 28.083529249207935 21.967873503341405 54-55 21.803540598312722 27.897975385789774 28.36205945598615 21.93642455991135 56-57 22.127259575630987 28.018578047702114 27.85400822427307 22.00015415239383 58-59 21.96724000454467 27.995066885438536 28.164506755076157 21.87318635494064 60-61 22.22028785438556 27.986489250198122 27.817099982104963 21.976122913311347 62-63 22.238854599434596 28.09399816883364 27.687450093814363 21.9796971379174 64-65 22.29817996266564 28.022654216425792 27.757665636077405 21.921500184831164 66-67 22.34386877715557 28.19497007047362 27.58490088021402 21.87626027215679 68-69 22.33050724507077 28.182640795349105 27.506023444938833 21.980828514641296 70-71 22.565328164481564 28.168750875592718 27.24145344812247 22.024467511803245 72-73 22.326536489008618 28.417662213579405 27.37321385917919 21.882587438232786 74-75 22.443486905319997 28.117878078978052 27.31316414251272 22.125470873189236 76-77 22.426743757927568 28.417182284030872 27.079449027235757 22.076624930805796 78-79 22.539684609149944 28.524283060558965 26.94985964719878 21.98617268309231 80-81 22.520739998541195 28.29995354854561 26.811126850860116 22.36817960205308 82-83 22.47150265160579 28.523194648112582 26.689708435849134 22.315594264432487 84-85 22.449756978495692 28.353113876001256 26.789695131491353 22.407434014011702 86-87 22.5611304833236 28.444134591306398 26.434102968134855 22.560631957235152 88-89 22.524802119283585 28.467953748302605 26.448414237926755 22.558829894487054 90-91 22.64119222885009 28.427684791866483 26.20683024766251 22.724292731620917 92-93 22.483873936753618 28.358810340111006 26.237597056785727 22.91971866634964 94-95 22.60149383419788 28.266798731492464 26.115840607402745 23.015866826906915 96-97 22.61077419023302 28.419086450565178 25.95346222314602 23.016677136055783 98-99 22.539594517168936 28.27241682319882 26.006107374537144 23.181881285095095 100-101 22.576551548518825 28.037658202442877 25.943257537290805 23.44253271174749 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 1.0 3 2.0 4 3.0 5 6.5 6 17.0 7 24.5 8 41.5 9 65.5 10 105.0 11 183.5 12 291.5 13 479.0 14 725.0 15 1058.5 16 1690.0 17 2667.0 18 3946.0 19 5777.0 20 8355.0 21 11601.0 22 15667.5 23 20591.0 24 26325.5 25 32869.0 26 40434.0 27 48777.0 28 57303.5 29 66177.5 30 75131.0 31 83960.0 32 92735.0 33 101834.0 34 110896.5 35 118867.0 36 126475.0 37 132969.5 38 136935.0 39 138016.0 40 137197.5 41 134572.0 42 130054.5 43 123742.0 44 116988.0 45 110722.0 46 104760.0 47 99558.0 48 94133.0 49 89040.5 50 84688.5 51 80651.5 52 76912.0 53 74702.5 54 74084.5 55 73948.5 56 74409.5 57 74670.0 58 73900.0 59 72551.0 60 70003.5 61 66524.5 62 62274.5 63 57088.5 64 51333.0 65 45140.0 66 39197.5 67 33374.0 68 27753.0 69 22622.5 70 18114.0 71 14502.0 72 11355.0 73 8551.0 74 6274.0 75 4413.5 76 3032.0 77 2055.5 78 1329.0 79 856.0 80 540.5 81 355.5 82 247.5 83 152.5 84 101.5 85 72.0 86 64.0 87 54.0 88 32.5 89 19.5 90 13.0 91 10.5 92 7.0 93 3.0 94 2.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.713872856751729 2 0.0026842130692544867 3 3.8345900989349813E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.19546183930971242 14-15 1.2781966996449938E-5 16-17 3.5789507590059825E-4 18-19 0.11278807677667425 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0018278212804923408 42-43 0.0 44-45 0.0 46-47 0.02509100121403123 48-49 2.5563933992899876E-5 50-51 1.022557359715995E-4 52-53 1.406016369609493E-4 54-55 0.0019812048844497403 56-57 0.001214286864662744 58-59 0.099315883562416 60-61 0.0015593999735668922 62-63 0.001188722930669844 64-65 0.0021218065214106893 66-67 0.0039751917358959304 68-69 0.004358650745789428 70-71 0.0031954917491124842 72-73 0.038729359999243304 74-75 0.04443011727965998 76-77 0.15560766621478153 78-79 2.939852409183485E-4 80-81 0.11391288987236184 82-83 0.0043203048448000785 84-85 0.004422560580771678 86-87 0.005892486785363421 88-89 0.0064037654652214174 90-91 0.021320320950078495 92-93 0.00999549819122385 94-95 0.01407294566309138 96-97 0.009151888369458154 98-99 0.010839108012989547 100-101 0.01214286864662744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3911761.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.42052719310036 #Duplication Level Percentage of deduplicated Percentage of total 1 98.33684844551709 95.80027618070287 2 1.1558219937505192 2.252015759451118 3 0.29996417998914887 0.8766800566076679 4 0.09973287283278558 0.3886411619940963 5 0.05152766724154328 0.2509926253850896 6 0.030088531177373565 0.17587443418594614 7 0.011064224335393672 0.07545177974157474 8 0.006460723806634769 0.05035256954330988 9 0.0014371824951334065 0.012600996871373267 >10 0.006981561336851209 0.1016214188346261 >50 0.0 0.0 >100 7.2617517530202E-5 0.015493016682329806 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.0451147194319898E-4 0.0 0.0 2 2.5563933992899874E-4 0.0 0.0 3 2.812032739218986E-4 0.0 0.0 4 3.578950759005982E-4 0.0 0.0 5 4.0902294388639796E-4 0.0 0.0 6 4.601508118721977E-4 0.0 0.0 7 5.112786798579975E-4 0.0 2.5563933992899872E-5 8 5.879704818366971E-4 0.0 2.5563933992899872E-5 9 6.646622838153967E-4 0.0 2.5563933992899872E-5 10-11 7.541360527905462E-4 0.0 3.8345900989349805E-5 12-13 9.586475247337453E-4 0.0 5.1127867985799745E-5 14-15 0.0011248130956875945 1.2781966996449936E-5 7.669180197869962E-5 16-17 0.0013037606336378936 2.5563933992899872E-5 8.947376897514955E-5 18-19 0.001418798336605943 2.5563933992899872E-5 1.0225573597159949E-4 20-21 0.0014954901385846424 5.1127867985799745E-5 1.2781966996449937E-4 22-23 0.001610527841552692 6.390983498224968E-5 1.6616557095384916E-4 24-25 0.0018406032474887909 8.947376897514955E-5 1.789475379502991E-4 26-27 0.0023007540593609887 1.1503770296804943E-4 1.789475379502991E-4 28-29 0.003425567155048583 1.406016369609493E-4 1.789475379502991E-4 30-31 0.0064037654652214174 1.789475379502991E-4 1.789475379502991E-4 32-33 0.01211730471263454 1.789475379502991E-4 1.9172950494674904E-4 34-35 0.021345884884071395 1.789475379502991E-4 2.0451147194319898E-4 36-37 0.036224094467939116 1.789475379502991E-4 2.0451147194319898E-4 38-39 0.05924441702854546 1.789475379502991E-4 2.3007540593609886E-4 40-41 0.09447151807076148 2.0451147194319898E-4 2.3007540593609886E-4 42-43 0.14775953847896128 2.3007540593609886E-4 2.5563933992899874E-4 44-45 0.22217615033229282 2.3007540593609886E-4 2.5563933992899874E-4 46-47 0.3260679780794379 2.3007540593609886E-4 2.812032739218986E-4 48-49 0.4615823921758001 2.3007540593609886E-4 3.067672079147985E-4 50-51 0.6366442121591784 2.428573729325488E-4 3.067672079147985E-4 52-53 0.8482752397194 2.812032739218986E-4 3.323311419076984E-4 54-55 1.0974980322161807 2.812032739218986E-4 3.323311419076984E-4 56-57 1.399881025451197 2.812032739218986E-4 3.4511310890414826E-4 58-59 1.746630226130891 2.9398524091834856E-4 3.578950759005982E-4 60-61 2.155116327403438 3.067672079147985E-4 3.578950759005982E-4 62-63 2.6106400672229206 3.323311419076984E-4 3.7067704289704814E-4 64-65 3.1189661127047383 3.323311419076984E-4 3.96240976889948E-4 66-67 3.682625293314188 3.323311419076984E-4 4.3458687787929784E-4 68-69 4.297885274688305 3.323311419076984E-4 4.473688448757478E-4 70-71 4.977937046767428 3.323311419076984E-4 4.601508118721977E-4 72-73 5.691285842872302 3.4511310890414826E-4 4.601508118721977E-4 74-75 6.4489752824878614 3.578950759005982E-4 4.601508118721977E-4 76-77 7.2656407178250415 3.578950759005982E-4 4.601508118721977E-4 78-79 8.143033278362354 4.0902294388639796E-4 4.601508118721977E-4 80-81 9.069738667571972 4.0902294388639796E-4 4.601508118721977E-4 82-83 10.034265897124083 4.0902294388639796E-4 4.601508118721977E-4 84-85 11.025226234424853 4.0902294388639796E-4 4.984967128615475E-4 86-87 12.07398662648357 4.0902294388639796E-4 5.112786798579975E-4 88-89 13.167841798105764 4.218049108828479E-4 5.112786798579975E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 4345 0.0 11.031768 94-95 TATGCCG 2675 0.0 10.733593 94-95 CGTATGC 3380 0.0 10.600926 94-95 GTATGCC 3425 0.0 10.184514 94-95 TATCTCG 6030 0.0 9.7986355 94-95 TCGTATG 3875 0.0 9.736637 94-95 TCTCGTA 4780 0.0 9.680332 94-95 ATCTCGT 5880 0.0 8.475211 92-93 GATGTAT 9970 0.0 8.425422 94-95 GTATCTC 7660 0.0 8.333109 94-95 ATGCCGT 2900 0.0 8.264316 94-95 CGATGTA 10150 0.0 8.20587 94-95 ATGTATC 9080 0.0 8.179788 94-95 CCGTCTT 2400 0.0 7.7119985 94-95 GCCGTCT 2355 0.0 7.65784 94-95 CCGATGT 11465 0.0 7.5548763 92-93 TGTATCT 9160 0.0 7.3830013 94-95 ACCGATG 12950 0.0 7.347814 94-95 TGCCGTC 3175 0.0 7.174821 94-95 CACCGAT 14590 0.0 6.9284782 94-95 >>END_MODULE