##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib19_GTGAAA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1675741 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.5285715393966 27.0 15.0 33.0 2.0 33.0 2 24.553359976273182 27.0 15.0 33.0 2.0 33.0 3 25.417100852697402 27.0 22.0 33.0 6.0 33.0 4 27.432476140405946 33.0 15.0 37.0 2.0 37.0 5 27.47090749704161 33.0 15.0 37.0 6.0 37.0 6 28.521301919568717 33.0 22.0 37.0 15.0 37.0 7 28.184179416747575 33.0 22.0 37.0 6.0 37.0 8 27.94536804911976 33.0 22.0 37.0 2.0 37.0 9 28.68487910721287 33.0 22.0 37.0 15.0 37.0 10-11 28.283850248934648 33.0 22.0 37.0 10.5 37.0 12-13 28.57816064654383 33.0 22.0 37.0 10.5 37.0 14-15 28.80474249899 33.0 22.0 37.0 6.0 40.0 16-17 29.644205757333623 33.0 24.5 37.0 15.0 40.0 18-19 29.91216154525073 33.0 27.0 37.0 15.0 40.0 20-21 29.90911483337819 33.0 27.0 37.0 15.0 40.0 22-23 29.898017951461473 33.0 27.0 37.0 15.0 40.0 24-25 29.444667463528077 33.0 24.5 37.0 10.5 40.0 26-27 28.90130545233422 33.0 22.0 37.0 6.0 40.0 28-29 29.037469095761217 33.0 22.0 37.0 15.0 40.0 30-31 28.90498113968686 33.0 22.0 37.0 10.5 40.0 32-33 28.69238981441643 33.0 22.0 37.0 6.0 40.0 34-35 28.711465853016666 33.0 22.0 37.0 10.5 40.0 36-37 28.92427887125755 33.0 22.0 37.0 6.0 40.0 38-39 28.919076695026263 33.0 22.0 37.0 10.5 40.0 40-41 28.957720494992962 33.0 22.0 37.0 10.5 40.0 42-43 28.820902215795876 33.0 22.0 37.0 15.0 40.0 44-45 28.512314552189153 33.0 22.0 37.0 10.5 40.0 46-47 28.107942695201704 33.0 22.0 37.0 6.0 40.0 48-49 27.92654652479112 33.0 22.0 37.0 6.0 40.0 50-51 27.143161144830856 30.0 22.0 35.0 6.0 38.5 52-53 26.8641836656142 30.0 22.0 37.0 6.0 37.0 54-55 27.74705697360153 33.0 22.0 37.0 6.0 40.0 56-57 27.63413588376724 33.0 22.0 37.0 6.0 40.0 58-59 27.802017137493202 33.0 22.0 37.0 6.0 40.0 60-61 27.547488543874024 33.0 22.0 37.0 6.0 40.0 62-63 27.38174067472241 33.0 22.0 37.0 6.0 40.0 64-65 27.330813651990372 33.0 22.0 37.0 6.0 37.0 66-67 27.18635009825504 30.0 22.0 37.0 6.0 37.0 68-69 26.889916759212788 27.0 22.0 37.0 6.0 37.0 70-71 26.765810169948697 27.0 22.0 35.0 6.0 37.0 72-73 26.510409723220953 27.0 22.0 33.0 6.0 37.0 74-75 26.43967265824492 27.0 22.0 33.0 6.0 37.0 76-77 26.047751412658638 27.0 22.0 33.0 6.0 37.0 78-79 25.4555838282885 27.0 15.0 33.0 6.0 37.0 80-81 25.323602215378152 27.0 15.0 33.0 6.0 37.0 82-83 24.88260357656702 27.0 15.0 33.0 6.0 37.0 84-85 24.95731828486622 27.0 15.0 33.0 6.0 37.0 86-87 24.641435341141623 27.0 15.0 33.0 6.0 37.0 88-89 24.258298269243276 27.0 15.0 33.0 6.0 37.0 90-91 24.092752997032356 27.0 15.0 33.0 6.0 37.0 92-93 23.943430995601346 27.0 15.0 33.0 6.0 37.0 94-95 23.6925219947474 27.0 15.0 33.0 6.0 37.0 96-97 23.524050852727242 27.0 15.0 33.0 6.0 37.0 98-99 23.24140275854085 27.0 15.0 33.0 6.0 33.0 100-101 22.736943835592733 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.288803630532627 2306 2 8.37905899554972 2306 3 7.427571429024891 2306 4 8.979524119086033 2306 5 8.767236907851412 2306 6 7.89376082971415 2306 7 8.02401842816283 2306 8 8.453922194604836 2306 9 7.262009622914928 2306 10-11 7.578907175324122 2306 12-13 7.2321329062837485 2306 14-15 7.742793313566722 2306 16-17 6.534977038812041 2306 18-19 5.985666246875393 2306 20-21 5.723717060664072 2306 22-23 5.411682840140067 2306 24-25 5.342122318861346 2306 26-27 5.826297688647124 2306 28-29 5.054718331527688 2306 30-31 4.924110203815445 2306 32-33 4.944226836414529 2306 34-35 4.311069636818651 2306 36-37 4.220432288386242 2306 38-39 4.124819861149231 2306 40-41 3.103811787889164 2306 42-43 2.799377601645336 2306 44-45 2.6253675323359786 2306 46-47 2.982018238045672 2306 48-49 2.6690784450965666 2306 50-51 2.4897692192864156 2306 52-53 3.040045806182654 2306 54-55 2.6757136051990322 2306 56-57 2.956720618707859 2306 58-59 2.5127271430437084 2306 60-61 2.311113771490387 2306 62-63 1.8918884031501548 2306 64-65 1.6510533029836694 2306 66-67 1.6387864679145032 2306 68-69 1.7618336557887453 2306 70-71 1.8163407385036123 2306 72-73 0.8872301433507204 2306 74-75 0.6367286780369703 2306 76-77 0.9373889569572178 2306 78-79 1.2471017756545777 2306 80-81 1.040877590816379 2306 82-83 0.972858104043894 2306 84-85 0.48831379984419243 2306 86-87 0.3724758538485169 2306 88-89 0.44611206876310305 2306 90-91 0.3182009154915768 2306 92-93 0.28928127028256156 2306 94-95 0.5635541414481899 2306 96-97 0.2482199378205472 2306 98-99 0.18266453295628793 2306 100-101 -0.01694100233097018 2307 1 8.223847397047752 2307 2 8.289526954100118 2307 3 7.555457477939598 2307 4 9.072875539405796 2307 5 9.028535132777982 2307 6 7.567510900726301 2307 7 7.952404786460196 2307 8 8.305212850020105 2307 9 7.279328142730005 2307 10-11 7.61075919521674 2307 12-13 7.265007462745704 2307 14-15 7.929031811517731 2307 16-17 6.606805060436589 2307 18-19 6.135906229556095 2307 20-21 6.163424981935009 2307 22-23 5.828135817813845 2307 24-25 5.8895382826996645 2307 26-27 6.595794323822787 2307 28-29 5.810809369871173 2307 30-31 5.592346085448554 2307 32-33 5.7131348000624556 2307 34-35 5.271374267424964 2307 36-37 5.254994669296952 2307 38-39 4.79266509918963 2307 40-41 4.241052670313003 2307 42-43 3.7780001041288394 2307 44-45 3.955964786737578 2307 46-47 4.373563800208554 2307 48-49 4.009889856787392 2307 50-51 3.677442546508864 2307 52-53 4.3506995478703985 2307 54-55 3.882961489036976 2307 56-57 4.243984416944301 2307 58-59 3.847054646471488 2307 60-61 3.6520525828160935 2307 62-63 3.4095192996535957 2307 64-65 3.235118330152531 2307 66-67 3.0682466834536513 2307 68-69 3.170418717596185 2307 70-71 2.7168987020747615 2307 72-73 2.1190577120329017 2307 74-75 1.7315176529593224 2307 76-77 2.1374354027415556 2307 78-79 2.3467858291662615 2307 80-81 2.1227648864741226 2307 82-83 2.2910592089161703 2307 84-85 1.5684981857695064 2307 86-87 1.4944294326305503 2307 88-89 1.663773366143559 2307 90-91 1.3142156752709084 2307 92-93 1.0829738559999846 2307 94-95 1.3562545530883447 2307 96-97 1.3083213585241609 2307 98-99 0.9774744660590038 2307 100-101 0.6773901553436374 1101 1 7.923705750270813 1101 2 7.767841797447275 1101 3 7.132499970428842 1101 4 8.608059518113233 1101 5 8.442517638050177 1101 6 7.282049726609603 1101 7 6.963218572281004 1101 8 7.749031183976122 1101 9 7.105755494529657 1101 10-11 7.368351269481334 1101 12-13 7.022604593066905 1101 14-15 7.337362610390116 1101 16-17 6.53932835689287 1101 18-19 5.757610491155699 1101 20-21 5.578081139208479 1101 22-23 5.043156898868226 1101 24-25 5.639781899717651 1101 26-27 6.082848310156763 1101 28-29 5.560829092351561 1101 30-31 5.236300199612003 1101 32-33 5.364473188423176 1101 34-35 5.236351117632918 1101 36-37 4.874239410226149 1101 38-39 4.6319619676765775 1101 40-41 4.055700336499022 1101 42-43 3.761092457827406 1101 44-45 3.8106599721366123 1101 46-47 3.9861886577898638 1101 48-49 3.659801588073808 1101 50-51 3.2131306256396215 1101 52-53 4.0957234618603096 1101 54-55 4.052149532659552 1101 56-57 4.022428749375006 1101 58-59 3.58759503471488 1101 60-61 3.6845239678514012 1101 62-63 3.3667645515918814 1101 64-65 3.2138858256522553 1101 66-67 2.879271665776752 1101 68-69 2.856464503083526 1101 70-71 2.811879079943594 1101 72-73 2.462121304102535 1101 74-75 1.9651071984236914 1101 76-77 2.0984808022703767 1101 78-79 2.65570533056961 1101 80-81 2.288238426701806 1101 82-83 2.45365060389377 1101 84-85 1.7400206995019118 1101 86-87 1.771656181717372 1101 88-89 1.7967448376335753 1101 90-91 1.7081974626602587 1101 92-93 1.4027728333642884 1101 94-95 1.5843079095380226 1101 96-97 1.4695261589218518 1101 98-99 1.3141370904534355 1101 100-101 1.065686001812427 2201 1 6.99766678923185 2201 2 7.744994644600123 2201 3 6.223094376023248 2201 4 8.417550027603742 2201 5 3.7362838718164113 2201 6 5.490286489846369 2201 7 6.3675642266266586 2201 8 7.4115686465135795 2201 9 6.253407842182003 2201 10-11 3.827214905844965 2201 12-13 6.1799222753845875 2201 14-15 6.832789683317188 2201 16-17 5.818351833416347 2201 18-19 1.6213592424044485 2201 20-21 4.741622597749938 2201 22-23 4.913613941825268 2201 24-25 5.276854826790579 2201 26-27 5.855378280186727 2201 28-29 4.806336085358552 2201 30-31 4.9450764233882225 2201 32-33 4.994180980630972 2201 34-35 4.658651317433122 2201 36-37 4.76980884079558 2201 38-39 4.097648780863391 2201 40-41 3.784186849985538 2201 42-43 3.4896164338513778 2201 44-45 3.2748008312774743 2201 46-47 3.711323522924726 2201 48-49 3.5747741155463366 2201 50-51 3.216035221044219 2201 52-53 4.158670514807362 2201 54-55 3.846262919272938 2201 56-57 3.779973704419959 2201 58-59 3.645162467282315 2201 60-61 3.51699649532393 2201 62-63 3.2239748413021694 2201 64-65 3.155254457020888 2201 66-67 2.615305631810717 2201 68-69 2.9484600075790333 2201 70-71 2.7507402188047294 2201 72-73 2.1316193061005357 2201 74-75 1.821348457164948 2201 76-77 2.0505262568158322 2201 78-79 2.316114920979196 2201 80-81 1.7409956694590463 2201 82-83 2.100161592904758 2201 84-85 1.765070649551859 2201 86-87 1.7167610768222659 2201 88-89 1.7322418406305786 2201 90-91 1.5568712888340812 2201 92-93 1.4495510551425141 2201 94-95 1.5524173001474146 2201 96-97 1.527900284795976 2201 98-99 1.250323404139749 2201 100-101 0.9481260617524896 2304 1 1.3991756467919352 2304 2 4.7645769548547925 2304 3 5.529847615668697 2304 4 4.083591279130587 2304 5 6.364383072508993 2304 6 6.286067400504546 2304 7 6.957877506374306 2304 8 6.418383532090473 2304 9 5.729467677046532 2304 10-11 6.647396161799971 2304 12-13 6.434749092111087 2304 14-15 6.7602990433985894 2304 16-17 5.55852985532329 2304 18-19 5.3130251005590985 2304 20-21 4.963179418730448 2304 22-23 4.596210981924976 2304 24-25 4.735519015678893 2304 26-27 5.528259244828632 2304 28-29 4.904637414054761 2304 30-31 4.9316337792305305 2304 32-33 5.163828693288288 2304 34-35 4.740078477963802 2304 36-37 4.823418361833067 2304 38-39 4.302073157625017 2304 40-41 3.731111593687231 2304 42-43 3.2247888956833926 2304 44-45 3.0582978820744167 2304 46-47 4.045538308494901 2304 48-49 3.7398125634022854 2304 50-51 3.416577396762488 2304 52-53 4.16866551447152 2304 54-55 3.8870891439198694 2304 56-57 4.5244239038114635 2304 58-59 3.7602500477914518 2304 60-61 3.700644800060818 2304 62-63 3.469294203547893 2304 64-65 3.3529585814272522 2304 66-67 3.3475882478073125 2304 68-69 3.445938693958464 2304 70-71 3.389444062097688 2304 72-73 2.955838246178601 2304 74-75 2.364331742111947 2304 76-77 2.768077236608004 2304 78-79 3.153195371656235 2304 80-81 2.9865380815116005 2304 82-83 3.094361302835523 2304 84-85 2.490536041506047 2304 86-87 2.4099165872669985 2304 88-89 2.627186127933456 2304 90-91 2.5894874595719166 2304 92-93 2.365567056217209 2304 94-95 2.6999311381415048 2304 96-97 2.567481649713521 2304 98-99 2.301439573633722 2304 100-101 1.9639677227595698 1102 1 6.98602649053273 1102 2 7.384439034632749 1102 3 5.970617354134461 1102 4 6.033793914435865 1102 5 6.3444042046426254 1102 6 5.662918911890554 1102 7 6.599473929124596 1102 8 6.117366552311488 1102 9 4.515480410136924 1102 10-11 5.799117208335506 1102 12-13 5.601533344054484 1102 14-15 5.916848892082285 1102 16-17 5.013360833131227 1102 18-19 5.1604334579492495 1102 20-21 5.291235033244725 1102 22-23 5.136002968920174 1102 24-25 1.5564150487037445 1102 26-27 5.172701131627228 1102 28-29 4.787004306026773 1102 30-31 4.672454404883851 1102 32-33 4.771723481702882 1102 34-35 4.750767610725884 1102 36-37 4.632165743152484 1102 38-39 3.5554342198253046 1102 40-41 3.709232262089774 1102 42-43 3.7027624164091257 1102 44-45 -4.02102800219842 1102 46-47 1.085575763059321 1102 48-49 -1.0850094560019414 1102 50-51 2.4655064905154873 1102 52-53 -0.675438165183671 1102 54-55 2.1445024139830196 1102 56-57 -0.9891884188598077 1102 58-59 2.5803749672006937 1102 60-61 3.665311753050954 1102 62-63 3.188215567895835 1102 64-65 2.816965861085233 1102 66-67 2.6992853122609866 1102 68-69 3.1306938956952735 1102 70-71 2.9555334647744473 1102 72-73 2.307295655894535 1102 74-75 2.1915391814733205 1102 76-77 2.522477965238128 1102 78-79 -1.6463595979659118 1102 80-81 1.1753137696595033 1102 82-83 2.201847532237757 1102 84-85 1.9398740596493873 1102 86-87 2.1416997547021204 1102 88-89 1.9898883771006446 1102 90-91 1.9543911110597882 1102 92-93 2.0112988933609373 1102 94-95 2.0741290089179465 1102 96-97 1.9930367169912344 1102 98-99 1.8719948916935891 1102 100-101 1.6033398240250705 2202 1 -20.3133821618171 2202 2 -7.449609878650154 2202 3 -0.6357199695393767 2202 4 1.3550179919271024 2202 5 0.7948739275143453 2202 6 1.4249138059120945 2202 7 3.953211173405972 2202 8 2.4863585085260915 2202 9 3.1996489002822806 2202 10-11 1.8062794373348972 2202 12-13 1.307487135925733 2202 14-15 2.779345025450194 2202 16-17 1.7975130471070173 2202 18-19 1.83647713904492 2202 20-21 1.145483839797869 2202 22-23 1.4034118027248859 2202 24-25 2.273874318393087 2202 26-27 3.4639812839159774 2202 28-29 -1.0273712784329732 2202 30-31 0.8042494080164495 2202 32-33 1.4297235941001318 2202 34-35 1.613436150045036 2202 36-37 3.0195598319620274 2202 38-39 2.6313642773026835 2202 40-41 1.8258380985074822 2202 42-43 0.7428093541674841 2202 44-45 0.9238726393469818 2202 46-47 -1.1212598233388 2202 48-49 0.7642567828407856 2202 50-51 1.856011625664845 2202 52-53 1.6627951048309626 2202 54-55 -0.6551737771599342 2202 56-57 1.234317501403389 2202 58-59 2.100963666847857 2202 60-61 2.1455039404419303 2202 62-63 -1.9573571472907219 2202 64-65 0.09510571517360233 2202 66-67 0.5776724998317526 2202 68-69 1.5887134037620392 2202 70-71 -2.33869937755912 2202 72-73 -4.513791258850961 2202 74-75 -1.0129745801649754 2202 76-77 0.1305897128701048 2202 78-79 1.728213465128242 2202 80-81 1.7621006394699101 2202 82-83 -0.7190968380292659 2202 84-85 -0.15076580932208827 2202 86-87 0.9143876277770495 2202 88-89 1.4224362751172848 2202 90-91 1.661667892383555 2202 92-93 1.5235643027976877 2202 94-95 1.7050179282874431 2202 96-97 1.2411839483397777 2202 98-99 1.2793338963254506 2202 100-101 0.8455372045500127 2305 1 5.671724221161625 2305 2 7.043993305513677 2305 3 6.7497258296759775 2305 4 7.713031850745143 2305 5 7.879973798910591 2305 6 6.248940459989701 2305 7 2.693847517803569 2305 8 5.950114497825389 2305 9 6.392814180524507 2305 10-11 4.744585584811389 2305 12-13 5.2393355571383005 2305 14-15 5.6882734902903636 2305 16-17 4.978675445974002 2305 18-19 4.465218043178144 2305 20-21 5.247185262036005 2305 22-23 5.174716208353068 2305 24-25 5.329248815780314 2305 26-27 6.0888718079355755 2305 28-29 5.006566953780911 2305 30-31 5.329289834090993 2305 32-33 5.351234742471966 2305 34-35 5.457535164082923 2305 36-37 4.5876424965015765 2305 38-39 4.61715505473137 2305 40-41 4.135627855788243 2305 42-43 3.9974156204803535 2305 44-45 3.0264342687619745 2305 46-47 3.6543848233264526 2305 48-49 3.282111813309566 2305 50-51 2.205014539598004 2305 52-53 4.004584813274853 2305 54-55 4.066147857030213 2305 56-57 4.6503231067906405 2305 58-59 4.26797653264957 2305 60-61 4.283259452225185 2305 62-63 3.7481173300191237 2305 64-65 4.013229212761601 2305 66-67 3.6006223009571734 2305 68-69 3.8714662566491107 2305 70-71 3.7251711070654068 2305 72-73 -1.2255852269550616 2305 74-75 1.9963060527608434 2305 76-77 3.2138858759201305 2305 78-79 3.6748718023956535 2305 80-81 3.1990423391014637 2305 82-83 3.4551479540613315 2305 84-85 3.007445296181828 2305 86-87 2.743819302284745 2305 88-89 2.8013382052588582 2305 90-91 2.608687486820493 2305 92-93 2.709703314230943 2305 94-95 3.015091753353783 2305 96-97 2.9352040094401275 2305 98-99 2.719729990461225 2305 100-101 2.3366109102373365 1103 1 -20.3133821618171 1103 2 -7.53850185889638 1103 3 -0.6730669832786056 1103 4 2.8651949446701472 1103 5 4.510809676590149 1103 6 4.570523442975883 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36-37 -24.997463886477146 1209 38-39 -25.250043526828915 1209 40-41 -22.088893277434213 1209 42-43 -22.595057759879417 1209 44-45 -25.14785651137987 1209 46-47 -18.996270392593715 1209 48-49 -18.505673738486422 1209 50-51 -20.83882614400583 1209 52-53 -23.243287527150677 1209 54-55 -20.359749181833504 1209 56-57 -24.027159162712906 1209 58-59 -24.263229141109296 1209 60-61 -24.02454196621453 1209 62-63 -24.110570613243286 1209 64-65 -23.98446582269939 1209 66-67 -12.021298518755698 1209 68-69 -23.610980551861527 1209 70-71 -23.471637403572892 1209 72-73 -13.832381598655086 1209 74-75 -15.11495448517573 1209 76-77 -12.733146297045403 1209 78-79 -13.616251957425415 1209 80-81 -22.42879454033116 1209 82-83 -14.647414604482762 1209 84-85 -11.922072396081752 1209 86-87 -12.555432767069691 1209 88-89 -9.653620598816765 1209 90-91 -12.25389304117553 1209 92-93 -15.680334153987982 1209 94-95 -20.938631650901538 1209 96-97 -16.856601251403674 1209 98-99 -8.36798244095855 1209 100-101 -4.41496141680669 1212 1 -20.3133821618171 1212 2 -20.12941094980547 1212 3 -21.019423106494234 1212 4 -22.91391850386479 1212 5 -23.08385598832345 1212 6 -24.124818405258527 1212 7 -23.660407801345368 1212 8 -23.305913870968936 1212 9 -24.19973901096485 1212 10-11 -23.8999179612879 1212 12-13 -24.183714088251772 1212 14-15 -24.418818708291198 1212 16-17 -25.40696285189834 1212 18-19 -20.19857082752562 1212 20-21 -25.770545234417895 1212 22-23 -25.916595848384524 1212 24-25 -21.879299019363266 1212 26-27 -24.590915426106974 1212 28-29 -19.44764992862746 1212 30-31 -14.243944555632755 1212 32-33 -24.61560922915924 1212 34-35 -20.734215815434013 1212 36-37 -24.997463886477146 1212 38-39 -25.250043526828915 1212 40-41 -19.888855108056422 1212 42-43 -15.94531363607869 1212 44-45 -13.788590777114138 1212 46-47 -16.401473679872474 1212 48-49 -19.128023087250867 1212 50-51 -11.260258485249489 1212 52-53 -4.420735079598231 1212 54-55 -3.416744073734055 1212 56-57 -3.438872449426192 1212 58-59 -2.216026343906499 1212 60-61 -13.15216434383691 1212 62-63 -10.73731886499154 1212 64-65 -15.321004284237853 1212 66-67 -11.281092969587885 1212 68-69 -7.20975677563775 1212 70-71 -14.004854186789675 1212 72-73 -10.74925481977359 1212 74-75 -7.727287906471414 1212 76-77 -17.802305911016337 1212 78-79 -8.800388575524298 1212 80-81 -7.0109623724989945 1212 82-83 -4.719488756745591 1212 84-85 -4.117796483315274 1212 86-87 -3.0250221399609494 1212 88-89 -2.719401516012777 1212 90-91 -2.794562277599482 1212 92-93 -2.8619874063959507 1212 94-95 -2.664156126426011 1212 96-97 -11.721295519399828 1212 98-99 -9.046914358098014 1212 100-101 -5.793342469716045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 312.0 3 623.0 4 327.0 5 1797.0 6 2131.0 7 2283.0 8 4617.0 9 8774.0 10 12262.0 11 17056.0 12 22715.0 13 27717.0 14 31690.0 15 34569.0 16 37121.0 17 39966.0 18 43987.0 19 47564.0 20 50747.0 21 55153.0 22 59227.0 23 63022.0 24 64732.0 25 65882.0 26 67555.0 27 69572.0 28 69033.0 29 69192.0 30 70422.0 31 73958.0 32 77923.0 33 86155.0 34 93102.0 35 98083.0 36 97210.0 37 82724.0 38 26469.0 39 69.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.2103813603305 25.261333204160298 14.89381284210672 27.63447259340249 2 25.286360173061368 26.660580205350936 27.38853888006874 20.664520741518952 3 20.626817964514522 25.111731049362675 27.964137622765296 26.297313363357507 4 25.29597639760623 25.535637505307022 25.877585568142177 23.290800528944573 5 24.21447712725316 26.563145678548413 25.600881391252706 23.62149580294572 6 23.458765499345606 27.241652355698793 25.107994220134206 24.1915879248214 7 24.732827655748938 27.145688139351858 25.450375293471634 22.671108911427567 8 23.205552392441973 26.97998744487224 26.37446133060465 23.439998832081137 9 22.90488376729736 25.706090639046415 27.30995303244824 24.07907256120798 10-11 24.04961143066944 25.927090982335905 26.017476696038838 24.00582089095582 12-13 23.97516545855657 26.083674755751655 25.770627166719194 24.170532618972583 14-15 23.981209040090192 26.368561338063707 25.66592911590839 23.984300505937707 16-17 24.32813487772194 26.27331927616513 25.410828963323173 23.987716882789755 18-19 24.30362919197762 26.24909983250262 25.31609816738526 24.131172808134497 20-21 24.215313017699216 26.386729912077882 25.477071897082283 23.920885173140622 22-23 24.23831441848897 26.202094818115523 25.539457066140343 24.020133697255165 24-25 24.05057265328997 26.238713779691004 25.65287668209466 24.05783688492437 26-27 23.943057626228594 26.38001934002553 25.662486306532234 24.01443672721365 28-29 24.36080977047922 26.247295219915923 25.491231776885698 23.900663232719157 30-31 24.25801210204243 26.133990429821797 25.516894303519127 24.091103164616648 32-33 24.08536660421811 26.27806184622884 25.59142518112954 24.045146368423513 34-35 24.366976175087245 26.16706513376834 25.520029251026966 23.945929440117443 36-37 24.210328993418408 26.220451015434204 25.55047190341663 24.018748087730756 38-39 24.14257661971865 26.28400262789513 25.72300388255777 23.850416869828447 40-41 24.431967891219372 26.177511599683328 25.421453400405706 23.96906710869159 42-43 24.255753481251062 26.223429517960774 25.540545365247464 23.9802716355407 44-45 24.114046698499454 26.238502881666243 25.77041158844843 23.877038831385875 46-47 24.435171755638603 26.27155736304089 25.324492854669007 23.968778026651503 48-49 24.283586694009536 26.192894137226602 25.522838719268353 24.00068044949551 50-51 24.28060981636246 26.280477107950418 25.513283112187995 23.925629963499127 52-53 24.338578862174565 26.18158983324993 25.56354157617905 23.91628972839645 54-55 24.220499113934206 26.13863708738383 25.59718282126266 24.0436809774193 56-57 24.159030606229663 26.27625218628949 25.67715720986313 23.887559997617718 58-59 24.436927651235884 26.260470829614686 25.34895691862947 23.953644600519965 60-61 24.402495169882457 26.08944303487216 25.392635224379195 24.115426570866184 62-63 24.3227779380461 26.257625211671765 25.5134531833542 23.90614366692793 64-65 24.552138908219284 26.16755586484188 25.39494413515812 23.885361091780716 66-67 24.584200909871363 26.158786686147188 25.33944635338789 23.917566050593553 68-69 24.570632470056612 26.223256076954403 25.3407900807694 23.865321372219586 70-71 24.8291607450026 26.175593462241782 25.109151487317092 23.88609430543853 72-73 24.766601305276712 26.183746933758435 25.169102438599015 23.88054932236584 74-75 24.771656422146325 26.24444188223571 25.182283561233838 23.80161813438413 76-77 25.115189416601424 26.22515019884637 24.95842630215296 23.70123408239925 78-79 25.129713136407194 26.111212964961574 25.05204330364118 23.707030594990055 80-81 25.16622738350019 26.230860659858372 24.9493986488751 23.653513307766335 82-83 25.364666119146506 26.151434002081537 24.881642992266652 23.60225688650531 84-85 25.484971562009868 26.120672384022892 24.72470431148534 23.6696517424819 86-87 25.487570633787314 26.203175014687524 24.809390698367633 23.499863653157526 88-89 25.791609952811285 26.161367367818144 24.610667719419162 23.43635495995141 90-91 25.94309814265304 26.11556692804274 24.35077074881616 23.59056418048806 92-93 25.981174223019764 26.199056858102516 24.443711859330904 23.376057059546813 94-95 26.32522289736755 26.117454106985345 24.22661424690857 23.330708748738534 96-97 26.405931159309613 26.09460339250238 24.217023843107096 23.28244160508091 98-99 26.53492336953114 26.21696589324583 24.134940089991908 23.113170647231122 100-101 27.255957728568507 25.990542281599797 23.605805981594173 23.147694008237522 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 115.0 1 122.5 2 126.5 3 131.0 4 128.0 5 140.5 6 186.5 7 210.0 8 270.5 9 366.0 10 474.0 11 638.5 12 870.0 13 1167.5 14 1472.0 15 1915.0 16 2437.5 17 2948.0 18 3737.0 19 4692.5 20 5780.5 21 7167.5 22 8784.0 23 10678.0 24 12722.5 25 15193.5 26 17905.0 27 20461.0 28 23255.0 29 26100.5 30 28867.0 31 31535.5 32 34454.5 33 37284.0 34 39555.5 35 41870.5 36 43674.0 37 45159.5 38 46279.5 39 46410.5 40 45962.0 41 45259.5 42 44251.5 43 42973.5 44 41467.0 45 39667.5 46 38127.0 47 36838.5 48 35020.5 49 33173.5 50 31515.0 51 29903.5 52 28682.0 53 27798.0 54 27289.0 55 26955.0 56 26843.5 57 26868.5 58 26954.0 59 27079.5 60 27293.0 61 27417.5 62 27414.5 63 27242.5 64 26993.0 65 26715.0 66 26249.5 67 25549.0 68 24493.5 69 23393.0 70 22148.5 71 20584.5 72 18716.0 73 16680.0 74 14702.0 75 12921.0 76 11178.0 77 9355.5 78 7755.0 79 6350.5 80 5080.5 81 4076.5 82 3329.0 83 2722.0 84 2167.0 85 1721.0 86 1382.0 87 1058.0 88 811.0 89 641.5 90 491.0 91 371.5 92 273.5 93 206.5 94 160.5 95 106.0 96 60.0 97 39.0 98 18.5 99 8.5 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.764119872939792 2 11.105296104827655 3 9.751148894727764 4 11.730273353698454 5 9.166213633252394 6 5.343606201674364 7 9.690757700623188 8 10.07237992028601 9 5.4195129199560075 10-11 6.379804516330388 12-13 5.325464973405794 14-15 7.344780607504382 16-17 2.629911185559105 18-19 3.1288844755842344 20-21 3.047278785922168 22-23 3.0672699420733873 24-25 3.474671801907335 26-27 4.316329313420153 28-29 1.710109139777567 30-31 2.2331911673701366 32-33 3.0396702115661074 34-35 1.9934763188344737 36-37 3.0650321260863103 38-39 1.3550423364947208 40-41 1.1411369656766768 42-43 0.5493987436005922 44-45 1.2875796438709801 46-47 1.3569221019238653 48-49 1.2503423858460228 50-51 1.5221027593166248 52-53 2.451393144883368 54-55 1.8060666893034187 56-57 1.8057086387454864 58-59 1.575243429623074 60-61 1.3933239086469806 62-63 0.6045982046151523 64-65 1.2124188642516953 66-67 0.5646158923127141 68-69 0.796901191771282 70-71 0.9893533666598836 72-73 0.08712563576352192 74-75 0.1096828209132557 76-77 0.4546645334810093 78-79 0.8870404197307341 80-81 0.28864842478640795 82-83 0.490678452099698 84-85 0.0675820428097182 86-87 0.10153717072029628 88-89 0.08354513018419911 90-91 0.09100451680778832 92-93 0.16840311241414993 94-95 0.44998003868139524 96-97 0.3650922189049501 98-99 0.06838765656506585 100-101 0.23147968570322025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1675741.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.4902816621179 #Duplication Level Percentage of deduplicated Percentage of total 1 99.1342691685013 97.63762092773904 2 0.5024547570834992 0.9897382109524974 3 0.18701168856039926 0.55256501641266 4 0.0926641978121172 0.36506091770038523 5 0.05380951273167636 0.26498570325220616 6 0.023544321518731787 0.13913321147540142 7 0.0018389962859654248 0.012678628352622639 8 0.0032259097933511204 0.025417661133098915 9 0.0 0.0 >10 0.0011814477129637147 0.012799722982074739 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1935018597742729E-4 0.0 0.0 2 1.7902527896614096E-4 0.0 0.0 3 1.7902527896614096E-4 0.0 0.0 4 2.3870037195485459E-4 0.0 0.0 5 3.580505579322819E-4 0.0 0.0 6 4.177256509209956E-4 0.0 5.9675092988713647E-5 7 4.7740074390970917E-4 0.0 5.9675092988713647E-5 8 5.967509298871365E-4 5.9675092988713647E-5 5.9675092988713647E-5 9 7.161011158645638E-4 1.1935018597742729E-4 5.9675092988713647E-5 10-11 9.249639413250616E-4 1.1935018597742729E-4 5.9675092988713647E-5 12-13 0.0011338267667855594 1.1935018597742729E-4 5.9675092988713647E-5 14-15 0.001342689592246057 1.1935018597742729E-4 5.9675092988713647E-5 16-17 0.0014322022317291277 1.1935018597742729E-4 5.9675092988713647E-5 18-19 0.0015813899642009117 1.1935018597742729E-4 5.9675092988713647E-5 20-21 0.001700740150178339 1.1935018597742729E-4 5.9675092988713647E-5 22-23 0.0018797654291444798 1.1935018597742729E-4 1.1935018597742729E-4 24-25 0.0021781408940880482 1.1935018597742729E-4 1.1935018597742729E-4 26-27 0.0024765163590316167 1.1935018597742729E-4 1.1935018597742729E-4 28-29 0.0031926174748961806 1.4918773247178413E-4 1.1935018597742729E-4 30-31 0.0043562817881760965 2.3870037195485459E-4 1.4918773247178413E-4 32-33 0.006802960600713356 2.3870037195485459E-4 1.7902527896614096E-4 34-35 0.011010054656417669 2.3870037195485459E-4 1.7902527896614096E-4 36-37 0.017454964699198744 2.3870037195485459E-4 1.7902527896614096E-4 38-39 0.026644929019460646 2.6853791844921144E-4 1.7902527896614096E-4 40-41 0.04031052531387607 2.9837546494356827E-4 1.7902527896614096E-4 42-43 0.057855002652557885 2.9837546494356827E-4 1.7902527896614096E-4 44-45 0.0826500037893684 2.9837546494356827E-4 1.7902527896614096E-4 46-47 0.11657529415345211 2.9837546494356827E-4 1.7902527896614096E-4 48-49 0.1561100432584749 2.9837546494356827E-4 1.7902527896614096E-4 50-51 0.2055806953461185 2.9837546494356827E-4 1.7902527896614096E-4 52-53 0.2702684961458841 2.9837546494356827E-4 2.3870037195485459E-4 54-55 0.3470106657293699 3.282130114379251E-4 2.3870037195485459E-4 56-57 0.43813453272313563 3.580505579322819E-4 2.3870037195485459E-4 58-59 0.5495180937865696 3.580505579322819E-4 2.3870037195485459E-4 60-61 0.6770139299569564 3.580505579322819E-4 2.3870037195485459E-4 62-63 0.815370633051289 3.580505579322819E-4 2.3870037195485459E-4 64-65 0.981446416838879 3.580505579322819E-4 2.6853791844921144E-4 66-67 1.165663428895038 3.580505579322819E-4 3.282130114379251E-4 68-69 1.3721392506359873 3.8788810442663875E-4 3.580505579322819E-4 70-71 1.5966966255525168 4.177256509209956E-4 3.580505579322819E-4 72-73 1.8284746867206807 4.475631974153524E-4 3.8788810442663875E-4 74-75 2.085137261665138 4.7740074390970917E-4 4.177256509209956E-4 76-77 2.3597023645062096 4.7740074390970917E-4 4.177256509209956E-4 78-79 2.6644332268530757 4.7740074390970917E-4 4.177256509209956E-4 80-81 3.0044619067027662 4.7740074390970917E-4 4.177256509209956E-4 82-83 3.357052193626581 5.07238290404066E-4 4.177256509209956E-4 84-85 3.7273659831680432 5.669133833927797E-4 4.475631974153524E-4 86-87 4.1281140701337495 5.967509298871365E-4 4.7740074390970917E-4 88-89 4.5499871400174605 5.967509298871365E-4 4.7740074390970917E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGAATT 1050 0.0022433675 6.225423 2 GTGTAGG 3500 0.0 5.7022023 94-95 TCGTGTA 3635 0.0 5.55282 92-93 GTCGTGT 4255 0.0 5.223415 92-93 CGTGTAG 3585 0.0 5.1241736 94-95 >>END_MODULE