FastQCFastQC Report
Thu 11 Sep 2014
NYCLib19_GTGAAA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib19_GTGAAA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675741
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAATT10500.00224336756.2254232
GTGTAGG35000.05.702202394-95
TCGTGTA36350.05.5528292-93
GTCGTGT42550.05.22341592-93
CGTGTAG35850.05.124173694-95
Produced by FastQC (version 0.11.2)