##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib19_GTGAAA_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1675741 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.248795607435756 33.0 33.0 33.0 27.0 33.0 2 31.66166072203282 33.0 33.0 33.0 27.0 33.0 3 31.808416694465315 33.0 33.0 33.0 27.0 33.0 4 35.624554749212436 37.0 37.0 37.0 33.0 37.0 5 35.12934278029839 37.0 37.0 37.0 33.0 37.0 6 34.916462030826956 37.0 33.0 37.0 33.0 37.0 7 34.698945720132166 37.0 33.0 37.0 27.0 37.0 8 34.73899546528968 37.0 33.0 37.0 27.0 37.0 9 34.65575945208717 37.0 33.0 37.0 27.0 37.0 10-11 34.505377322629215 37.0 33.0 37.0 27.0 37.0 12-13 34.50510222045054 37.0 33.0 37.0 27.0 37.0 14-15 35.39909598201632 37.0 33.0 40.0 27.0 40.0 16-17 35.292245042640836 37.0 33.0 40.0 27.0 40.0 18-19 35.50158676072257 37.0 33.0 40.0 27.0 40.0 20-21 35.14743865549629 37.0 33.0 40.0 27.0 40.0 22-23 35.16160790957552 37.0 33.0 40.0 27.0 40.0 24-25 34.98174598580569 37.0 33.0 40.0 27.0 40.0 26-27 34.55179589208595 37.0 33.0 40.0 27.0 40.0 28-29 34.147324974444146 37.0 33.0 40.0 22.0 40.0 30-31 34.00021841084034 37.0 33.0 37.0 22.0 40.0 32-33 33.97870345118965 37.0 33.0 37.0 22.0 40.0 34-35 33.78422321826583 37.0 33.0 37.0 22.0 40.0 36-37 33.43244361748027 37.0 33.0 37.0 22.0 40.0 38-39 33.2892612879914 37.0 33.0 37.0 22.0 40.0 40-41 32.96127355002951 37.0 33.0 37.0 22.0 40.0 42-43 32.518479586045814 37.0 33.0 37.0 22.0 40.0 44-45 32.35115957656941 37.0 33.0 37.0 18.5 40.0 46-47 32.18413257179958 37.0 30.0 37.0 18.5 40.0 48-49 32.17680327687871 37.0 30.0 37.0 22.0 40.0 50-51 32.50112726250656 37.0 33.0 37.0 22.0 40.0 52-53 32.34001823670842 37.0 33.0 37.0 22.0 40.0 54-55 31.962347701703308 33.0 27.0 37.0 18.5 40.0 56-57 31.99539666332685 33.0 27.0 37.0 22.0 40.0 58-59 31.695523353549266 33.0 27.0 37.0 15.0 40.0 60-61 31.596754808768182 33.0 27.0 37.0 18.5 40.0 62-63 31.568754061636014 33.0 27.0 37.0 18.5 40.0 64-65 31.222648667067283 33.0 27.0 37.0 15.0 40.0 66-67 30.87084668812185 33.0 27.0 37.0 15.0 40.0 68-69 30.683788246513032 33.0 27.0 37.0 15.0 38.5 70-71 30.536146098949658 33.0 27.0 37.0 15.0 37.0 72-73 30.32209333065193 33.0 27.0 37.0 15.0 37.0 74-75 29.96750004923195 33.0 27.0 37.0 15.0 37.0 76-77 28.858130224181423 33.0 27.0 35.0 15.0 37.0 78-79 29.810553062794312 33.0 27.0 37.0 15.0 37.0 80-81 29.54133067102852 33.0 27.0 37.0 15.0 37.0 82-83 29.528180070786597 33.0 27.0 37.0 15.0 37.0 84-85 29.403489859113073 33.0 27.0 37.0 15.0 37.0 86-87 29.20226663905699 33.0 27.0 37.0 15.0 37.0 88-89 29.06260155954888 33.0 27.0 35.0 15.0 37.0 90-91 28.975775194376695 33.0 27.0 33.0 15.0 37.0 92-93 28.75105729346003 33.0 27.0 33.0 15.0 37.0 94-95 28.653530288988573 33.0 27.0 33.0 15.0 37.0 96-97 28.497526765771084 33.0 27.0 33.0 15.0 37.0 98-99 28.204649167144566 33.0 27.0 33.0 15.0 37.0 100-101 27.36712863145319 33.0 22.0 33.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1162874037547716 2306 2 0.5653189939151204 2306 3 0.463054228157862 2306 4 0.3525803466764472 2306 5 0.6620528905990852 2306 6 0.71785175697471 2306 7 0.8265847537682163 2306 8 0.6968676719969835 2306 9 1.0387095036847285 2306 10-11 1.138072686975697 2306 12-13 1.4262587453345361 2306 14-15 1.492326642863226 2306 16-17 1.4891260610882284 2306 18-19 1.3019700738549815 2306 20-21 1.5241473378568173 2306 22-23 1.146350969676682 2306 24-25 1.2209683414378887 2306 26-27 1.3774669707748828 2306 28-29 1.5992743912908907 2306 30-31 1.6094644190879634 2306 32-33 1.1026099045061883 2306 34-35 1.0008119530036197 2306 36-37 1.2815461720425603 2306 38-39 1.115905002972866 2306 40-41 1.0931275381923413 2306 42-43 1.0233411968571815 2306 44-45 0.84661546283224 2306 46-47 0.8251055305084023 2306 48-49 0.8588972543188085 2306 50-51 1.0195351719341303 2306 52-53 1.057082350191017 2306 54-55 1.3935796784332553 2306 56-57 1.0847731015415967 2306 58-59 1.0943736397828232 2306 60-61 0.8270371332428965 2306 62-63 0.6059338010651949 2306 64-65 0.4275913917411529 2306 66-67 0.8741726830173917 2306 68-69 0.6269799042446174 2306 70-71 0.6440962196338305 2306 72-73 0.29198422877520613 2306 74-75 0.5177557510696964 2306 76-77 0.5758057398911731 2306 78-79 0.35472864102452917 2306 80-81 0.897940369643166 2306 82-83 0.6911537157745542 2306 84-85 0.46075984423040595 2306 86-87 0.3908992144962937 2306 88-89 0.14998331923279906 2306 90-91 -0.0818089121707466 2306 92-93 -0.32409763163248684 2306 94-95 -0.2096343560472782 2306 96-97 -0.24356663045246663 2306 98-99 -0.13842177519943633 2306 100-101 -0.025572705765174675 2307 1 0.6002953908942779 2307 2 0.4648751104026836 2307 3 0.44873917680648034 2307 4 0.2990656675055021 2307 5 0.5277689924696602 2307 6 0.5660954921677188 2307 7 0.766910519125652 2307 8 0.8187659764571649 2307 9 0.9397009868329675 2307 10-11 1.1525668040989103 2307 12-13 1.328056043648516 2307 14-15 1.3126300528282613 2307 16-17 1.2597019273980692 2307 18-19 1.179127499729816 2307 20-21 1.3012948988005988 2307 22-23 1.1071418223905738 2307 24-25 1.0922032772659378 2307 26-27 1.090681525732144 2307 28-29 1.616331471240592 2307 30-31 1.6180335511429291 2307 32-33 1.266948025209956 2307 34-35 1.2403425358252917 2307 36-37 1.2129339644014934 2307 38-39 1.0154872212235233 2307 40-41 1.079906841643762 2307 42-43 1.1151403299715739 2307 44-45 0.7948176655139676 2307 46-47 0.7506924747135741 2307 48-49 0.973455052066992 2307 50-51 0.9033618285900076 2307 52-53 1.18946427425945 2307 54-55 1.3908662082345629 2307 56-57 0.9369932484177603 2307 58-59 0.9207690477149981 2307 60-61 0.750373452925011 2307 62-63 0.5736023148154494 2307 64-65 0.7759890889514871 2307 66-67 0.8713669737940997 2307 68-69 0.7735807637603216 2307 70-71 0.774077587581175 2307 72-73 0.5177906581000791 2307 74-75 0.529829582209711 2307 76-77 0.7465992872282534 2307 78-79 0.7076614428031291 2307 80-81 1.2525607271759043 2307 82-83 1.0549728336270832 2307 84-85 0.9007637134329372 2307 86-87 0.810374085407723 2307 88-89 0.5486655256608977 2307 90-91 0.41991507089688795 2307 92-93 0.48875407043277974 2307 94-95 0.32333209397475926 2307 96-97 0.35284038508729765 2307 98-99 0.36277023090530847 2307 100-101 0.3527218741269529 1101 1 1.1107838908742096 1101 2 0.47211206423278185 1101 3 0.38052255218356734 1101 4 0.32041797353046775 1101 5 0.5286715178734482 1101 6 0.5257317185431987 1101 7 0.733521942262783 1101 8 0.7059110875977765 1101 9 0.8658082246359129 1101 10-11 0.9188546782085041 1101 12-13 1.1724178457969145 1101 14-15 1.2129873346304763 1101 16-17 1.2934158286976682 1101 18-19 0.9871078868616507 1101 20-21 1.2689380662541083 1101 22-23 0.9729152523885034 1101 24-25 1.1292318675292847 1101 26-27 1.1477275844781119 1101 28-29 1.2113803181509155 1101 30-31 1.2862703999923113 1101 32-33 0.7024822998703897 1101 34-35 0.8908723024902088 1101 36-37 1.131905716869312 1101 38-39 0.8239013099445742 1101 40-41 1.005922530085698 1101 42-43 0.8864199214113171 1101 44-45 0.8673437099949055 1101 46-47 0.8405126643581582 1101 48-49 0.7065613388681378 1101 50-51 0.7118871505565991 1101 52-53 0.9629656745169655 1101 54-55 1.2843215867981144 1101 56-57 0.9455894709645314 1101 58-59 0.9822662116917869 1101 60-61 0.5137947745087388 1101 62-63 0.4973347498431906 1101 64-65 0.5310239564442085 1101 66-67 0.6627438113426187 1101 68-69 0.5915200771864306 1101 70-71 0.646194940585012 1101 72-73 0.43034893783664074 1101 74-75 0.6268336949837057 1101 76-77 0.6079365871859537 1101 78-79 0.5992198738354304 1101 80-81 1.2589113658600652 1101 82-83 1.0729392799371666 1101 84-85 0.8554815160012588 1101 86-87 0.6249426300970882 1101 88-89 0.4220394052233125 1101 90-91 0.28211425482619035 1101 92-93 0.5636057851692904 1101 94-95 0.6218231891397785 1101 96-97 0.48343601171411166 1101 98-99 0.5121449395066229 1101 100-101 0.25567391646505655 2201 1 1.4532414333317512 2201 2 0.7939452310659441 2201 3 0.29605701771803083 2201 4 0.232076315188813 2201 5 0.4738263630282944 2201 6 0.45691553472701685 2201 7 0.6533870771279169 2201 8 0.6098471515338417 2201 9 0.5726164164440988 2201 10-11 0.8794565763104032 2201 12-13 1.1191486150276901 2201 14-15 1.1588989230420594 2201 16-17 1.1267449995268422 2201 18-19 0.9116433513971103 2201 20-21 1.1399620422780856 2201 22-23 0.888364802838062 2201 24-25 0.8935450543424679 2201 26-27 1.0984268851774104 2201 28-29 1.3570796188502214 2201 30-31 1.3049716986936062 2201 32-33 0.8195752069633002 2201 34-35 0.9198982765161787 2201 36-37 0.9878572653208622 2201 38-39 0.647884826428097 2201 40-41 0.8871662863294532 2201 42-43 0.9852510902424854 2201 44-45 0.9306129407641421 2201 46-47 0.6115666104121047 2201 48-49 0.859348551655355 2201 50-51 0.677524013693457 2201 52-53 0.9169466934979909 2201 54-55 1.2843215867981144 2201 56-57 0.8843407197157767 2201 58-59 1.026736741162317 2201 60-61 0.7785100592240255 2201 62-63 0.7071848996933419 2201 64-65 0.773703776624032 2201 66-67 0.8988427124415175 2201 68-69 0.7757383589047109 2201 70-71 0.816849286239357 2201 72-73 0.5541176691053664 2201 74-75 0.7150804482304558 2201 76-77 0.6203341895835557 2201 78-79 0.622149444265002 2201 80-81 1.188107170055872 2201 82-83 0.9315656535635384 2201 84-85 0.9426743231940655 2201 86-87 1.0404870856415407 2201 88-89 0.8332232214071276 2201 90-91 0.6203984706104073 2201 92-93 0.7590203705838796 2201 94-95 0.7168057066222957 2201 96-97 0.651532914811014 2201 98-99 0.7016878965495827 2201 100-101 0.827926663717804 2304 1 -2.0084517649676386 2304 2 -0.40426420091082704 2304 3 -0.1485713528441721 2304 4 -0.09847464221380164 2304 5 0.325260663341993 2304 6 0.42544546086633517 2304 7 0.43057639083804133 2304 8 0.7099486011336893 2304 9 0.9190866520071879 2304 10-11 0.8880270524369109 2304 12-13 1.2392832509360616 2304 14-15 1.1556221855497384 2304 16-17 1.195092376091928 2304 18-19 0.7233591134010311 2304 20-21 1.1739868150862094 2304 22-23 0.9382434762812935 2304 24-25 1.05226984431593 2304 26-27 1.0221590761454635 2304 28-29 1.506303422439018 2304 30-31 1.4777520595636204 2304 32-33 0.8803785942554825 2304 34-35 0.9888749490787632 2304 36-37 1.1971833643374623 2304 38-39 1.0466052379980226 2304 40-41 1.0624646272627984 2304 42-43 1.0344042920744485 2304 44-45 1.221397495945702 2304 46-47 0.9660623103720525 2304 48-49 0.954382496721319 2304 50-51 0.8670513700084399 2304 52-53 1.1343073620005981 2304 54-55 1.629148229383567 2304 56-57 1.1421721661064517 2304 58-59 1.010767957189259 2304 60-61 0.8012428298330008 2304 62-63 0.5786452216552611 2304 64-65 0.5019574653046135 2304 66-67 0.49563607916017816 2304 68-69 0.4823671125772222 2304 70-71 0.4527106500623006 2304 72-73 0.5765317615941683 2304 74-75 0.7524073831240976 2304 76-77 0.8265418701989198 2304 78-79 0.8410133480595334 2304 80-81 1.4495381616896346 2304 82-83 1.0377890068733393 2304 84-85 0.8483099255852729 2304 86-87 0.8403639927969948 2304 88-89 0.6280471026130812 2304 90-91 0.43106192313085145 2304 92-93 0.575842445698374 2304 94-95 0.19500170486098156 2304 96-97 0.278718770577445 2304 98-99 0.5449622955763793 2304 100-101 0.7040397359888253 1102 1 1.4553839531213306 1102 2 0.1872279396427743 1102 3 0.2905575063361674 1102 4 0.17135168034652537 1102 5 0.2814714412616084 1102 6 0.15122131609162182 1102 7 -4.90604268700773 1102 8 -1.0946299835315259 1102 9 0.2808786117651181 1102 10-11 0.7382868527877378 1102 12-13 0.9120127008329533 1102 14-15 0.9369437975575252 1102 16-17 0.5236311199423724 1102 18-19 0.882238246697888 1102 20-21 1.1310816416329743 1102 22-23 0.6899739562119152 1102 24-25 1.0572726992465817 1102 26-27 1.0010530172153125 1102 28-29 1.0387672558319707 1102 30-31 1.394563631814954 1102 32-33 1.0446247978952385 1102 34-35 1.0477368431782779 1102 36-37 1.2064295862461236 1102 38-39 0.8936536980793193 1102 40-41 1.24101853429935 1102 42-43 1.3090886470212197 1102 44-45 1.1333259575947991 1102 46-47 0.8906461365504583 1102 48-49 0.7597730509033873 1102 50-51 0.9161046922741463 1102 52-53 0.9805594641695805 1102 54-55 1.332575076228082 1102 56-57 1.0861829733227317 1102 58-59 0.9665841268920587 1102 60-61 0.9112905249456702 1102 62-63 0.621163426613041 1102 64-65 0.4105365734568238 1102 66-67 0.6762005615640767 1102 68-69 0.748276583207641 1102 70-71 0.7351059750842808 1102 72-73 0.51423680922451 1102 74-75 0.7336683091712608 1102 76-77 0.5657055067195813 1102 78-79 0.6027179824805984 1102 80-81 1.3440995436953003 1102 82-83 1.2617210319542096 1102 84-85 0.8798656286206672 1102 86-87 0.6247750416941997 1102 88-89 0.6794498052807718 1102 90-91 0.22447970527987593 1102 92-93 0.47809619671852843 1102 94-95 0.2824982711383903 1102 96-97 0.1590237681842197 1102 98-99 0.2743947539623228 1102 100-101 0.40964755720340307 2202 1 1.5580568168770519 2202 2 0.8710230423136132 2202 3 0.4340487887625919 2202 4 -0.1395066083910521 2202 5 0.2937748919286207 2202 6 0.619697221288348 2202 7 0.6376966313839105 2202 8 -4.4709926513564575 2202 9 -0.6174352151683564 2202 10-11 0.5072499000486417 2202 12-13 -0.024238472253820476 2202 14-15 0.05134240916573418 2202 16-17 0.2802131457646837 2202 18-19 0.881601190038964 2202 20-21 1.1014428797267826 2202 22-23 -2.708279623890533 2202 24-25 -3.093073743844805 2202 26-27 -1.4180748991897119 2202 28-29 0.6081418389560511 2202 30-31 1.4802020612919833 2202 32-33 1.31169852358547 2202 34-35 -1.8989704293762415 2202 36-37 0.10658338235608511 2202 38-39 0.6229910591395296 2202 40-41 1.2531536272211525 2202 42-43 1.4195244221133692 2202 44-45 1.423443262699287 2202 46-47 0.6062022731426424 2202 48-49 1.0649826039076196 2202 50-51 -3.982843988617919 2202 52-53 -0.22772246290032427 2202 54-55 1.5912340698773946 2202 56-57 1.5139941726132982 2202 58-59 1.6715674804306957 2202 60-61 1.6921770272980297 2202 62-63 1.310020037012027 2202 64-65 1.304815648684638 2202 66-67 1.4366680317281997 2202 68-69 1.3677571817039471 2202 70-71 1.4758436201380505 2202 72-73 1.1644465062972529 2202 74-75 0.7148813588729901 2202 76-77 1.2199004840389094 2202 78-79 1.2848682633189412 2202 80-81 2.0192565486038703 2202 82-83 1.7695322241008853 2202 84-85 1.6287606469697558 2202 86-87 1.692415325725861 2202 88-89 1.511579035447717 2202 90-91 1.3775564847462327 2202 92-93 1.5331804339329231 2202 94-95 1.3491207290138298 2202 96-97 1.360123880081396 2202 98-99 1.5983175561019642 2202 100-101 1.4770409006667755 2305 1 1.4328203650383422 2305 2 0.7179872882994935 2305 3 0.13381925548026885 2305 4 -0.2144351166417735 2305 5 0.18852655645189031 2305 6 0.25568456711307164 2305 7 0.47536396548778725 2305 8 -0.007189995716075259 2305 9 0.4269550565274258 2305 10-11 0.3283034740934738 2305 12-13 0.9180329576354325 2305 14-15 0.6161232944153667 2305 16-17 0.7328564412765743 2305 18-19 0.7425264045142086 2305 20-21 0.7614191667139352 2305 22-23 0.5781408778268968 2305 24-25 0.8242760963501041 2305 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-1.014355024338201 1208 42-43 0.05744422231366997 1208 44-45 0.25327502123109724 1208 46-47 0.22581746795564328 1208 48-49 -1.1064810388083828 1208 50-51 0.05239647185860008 1208 52-53 -1.1661217015216288 1208 54-55 -1.059252522019893 1208 56-57 -6.33543214969124 1208 58-59 -1.8617627945323392 1208 60-61 -1.2111598102019414 1208 62-63 0.48227548661319375 1208 64-65 -0.012921292074210555 1208 66-67 0.9494170297231541 1208 68-69 0.6018743908944018 1208 70-71 -4.292681291583904 1208 72-73 -0.6455418718956345 1208 74-75 -5.583346030439923 1208 76-77 -1.0884718823932467 1208 78-79 -3.8212665164680324 1208 80-81 0.9775740717178927 1208 82-83 0.585178328151823 1208 84-85 -2.8675410576310725 1208 86-87 -1.0048669234929548 1208 88-89 -4.932042759224704 1208 90-91 -0.0560014573748866 1208 92-93 -1.9063091914530013 1208 94-95 -4.789379124806441 1208 96-97 -3.438535309647456 1208 98-99 -6.496441900482655 1208 100-101 -3.6877967050543425 1209 1 1.6509456159415912 1209 2 1.1938049939445783 1209 3 0.5305847541325583 1209 4 0.46152309482426546 1209 5 0.4013441282819059 1209 6 0.9318119590573986 1209 7 0.658086528619819 1209 8 8.31261763231339E-4 1209 9 -0.1641471973006432 1209 10-11 -2.9271877227867265 1209 12-13 -0.2914091291149603 1209 14-15 -1.0332157547329928 1209 16-17 -0.3141857678190192 1209 18-19 -16.645402998479426 1209 20-21 -14.108290836918194 1209 22-23 -3.374736624036956 1209 24-25 -1.944920630915668 1209 26-27 -1.1385361517856225 1209 28-29 -3.11997464763801 1209 30-31 -0.9572294963377814 1209 32-33 -0.7204598523170418 1209 34-35 -2.5586352654135887 1209 36-37 -4.086316862438174 1209 38-39 -1.755527987173398 1209 40-41 -9.846746970225315 1209 42-43 -5.817364030485841 1209 44-45 -2.850116895437395 1209 46-47 -2.648669380012567 1209 48-49 -3.4751305541445063 1209 50-51 -2.6771367971937607 1209 52-53 -1.1875371245707385 1209 54-55 -2.6526471048875564 1209 56-57 -0.49425087736638673 1209 58-59 -1.4236377899669321 1209 60-61 -2.1613037119105023 1209 62-63 -1.1322733778781462 1209 64-65 0.14828240554228245 1209 66-67 0.5725561687964849 1209 68-69 -1.2544393878768076 1209 70-71 -1.3531674894377943 1209 72-73 -0.6248267826692739 1209 74-75 0.14023303149624766 1209 76-77 -16.32315402031975 1209 78-79 -15.887554436759633 1209 80-81 -21.1908968447972 1209 82-83 -10.079128934866898 1209 84-85 -3.3696895393584754 1209 86-87 -2.192805659349318 1209 88-89 -1.8243995331967575 1209 90-91 -5.838736356826306 1209 92-93 -3.3037070906341484 1209 94-95 -0.3832229439723953 1209 96-97 0.005699125958358309 1209 98-99 1.5102555553059211 1209 100-101 0.493135040246937 1212 1 -28.632073251982934 1212 2 -16.321019803899087 1212 3 -5.776320604659592 1212 4 -1.2711404680279728 1212 5 -0.4677820285800962 1212 6 -2.5862313184198342 1212 7 -0.5464730627322254 1212 8 -1.5787542470675575 1212 9 0.2483506821783692 1212 10-11 0.9763097231635527 1212 12-13 0.2167360276150987 1212 14-15 0.032605216748358146 1212 16-17 -0.1945836718018299 1212 18-19 -1.1758042010504397 1212 20-21 -0.8345834122673779 1212 22-23 -0.6740128195395627 1212 24-25 0.40599260679002214 1212 26-27 -0.2431815064309788 1212 28-29 0.29442227619287564 1212 30-31 -1.0297136159917102 1212 32-33 0.11237240976049634 1212 34-35 -1.691745080127177 1212 36-37 -1.4130867906231934 1212 38-39 -0.27211517357190473 1212 40-41 -0.5834630842999182 1212 42-43 0.04143990143129628 1212 44-45 0.946452101603299 1212 46-47 -2.2803914315459366 1212 48-49 -1.2820500497432477 1212 50-51 -0.08677668560723362 1212 52-53 -1.1675288309775382 1212 54-55 -0.7139301614536286 1212 56-57 -1.1638760635010073 1212 58-59 0.15037310479868182 1212 60-61 0.5747338354478018 1212 62-63 -3.9619060093975698 1212 64-65 -3.4072752443549916 1212 66-67 -1.3232202246214193 1212 68-69 0.3474516456179977 1212 70-71 -0.991017846627777 1212 72-73 -1.404728484740783 1212 74-75 -0.15925521610520477 1212 76-77 1.1708237000730648 1212 78-79 -0.6884799263643657 1212 80-81 0.6685267504754506 1212 82-83 0.6918453738432575 1212 84-85 -3.3347032991835555 1212 86-87 -1.4387436835892302 1212 88-89 -0.28590265271874316 1212 90-91 0.4787551139670505 1212 92-93 -2.7709096993461912 1212 94-95 -1.9758166710000822 1212 96-97 0.3541902574683533 1212 98-99 -0.4083470684853836 1212 100-101 0.2233812091723486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 3.0 10 3.0 11 10.0 12 60.0 13 253.0 14 756.0 15 1723.0 16 3164.0 17 5210.0 18 7922.0 19 10972.0 20 14982.0 21 19610.0 22 24893.0 23 31205.0 24 37922.0 25 46376.0 26 55136.0 27 65214.0 28 75892.0 29 88219.0 30 101711.0 31 115056.0 32 130874.0 33 145918.0 34 159077.0 35 169178.0 36 169027.0 37 145473.0 38 49786.0 39 114.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.619323737107564 25.202742454134686 16.531044309179315 27.646889499578442 2 24.311508320144753 25.670230567431247 29.543973474839053 20.47428763758495 3 20.576667046599905 24.20976372727636 29.602209898593628 25.611359327530103 4 25.214099314870257 25.132881513312615 27.20187666232431 22.451142509492815 5 23.761130150781057 26.5568485822093 26.31528380579099 23.366737461218648 6 22.906403793903714 27.270503019261326 25.93479541289495 23.888297773940007 7 24.09178984103152 27.22884980435521 26.11573029483673 22.56363005977654 8 22.6912750836794 26.895982135664166 27.062535320195664 23.350207460460776 9 22.48008492959234 25.541118824448404 27.84284683611608 24.135949409843168 10-11 23.3342145355398 25.81830963138098 26.7747223467111 24.07275348636812 12-13 23.647294948621692 25.917004316052978 26.224401273950086 24.21129946137524 14-15 23.595277310322604 26.176972561375873 26.07504744172724 24.15270268657429 16-17 23.763259845226216 26.086179445927044 25.99645767591076 24.154103032935975 18-19 23.973265626166828 26.061324770368156 25.72689119557912 24.23851840788589 20-21 23.6710207603681 26.074643993314 26.09332229741947 24.161012948898428 22-23 23.819708361766036 25.94130773828048 26.034788799339754 24.204195100613727 24-25 23.758713309515013 25.821442573762894 26.135393237976512 24.28445087874558 26-27 23.742660709501052 26.052415021175708 26.109136197061478 24.095788072261765 28-29 24.004783555453976 25.97251007166382 25.997275235254136 24.02543113762807 30-31 23.987716478859202 25.87837261247412 25.887174688689957 24.246736219976714 32-33 23.805230044499716 25.972450396570828 26.009210253851876 24.213109305077573 34-35 24.11906135852736 25.83287035407023 25.906091693167383 24.14197659423503 36-37 23.995742778866187 25.88439979686598 25.96722882593432 24.152628598333514 38-39 23.903634272838108 25.931483445234075 26.114238417512013 24.050643864415804 40-41 24.048589567583164 25.805487168271114 25.949933446101486 24.195989818044236 42-43 23.806513058998974 25.859634633275668 26.128172551724877 24.20567975600048 44-45 23.791385422926336 25.85220508419857 26.238213423196065 24.118196069679026 46-47 24.06580379910036 25.824338165773714 26.040890706625024 24.068967328500893 48-49 23.969187362486206 25.792052590465946 26.053936736046918 24.184823311000926 50-51 23.903088755740377 25.903041612374718 26.143562289962137 24.050307341922775 52-53 24.009049141900977 25.891890085508546 26.035677393675265 24.063383378915212 54-55 23.84856912762572 25.799982514858637 26.20387137739052 24.147576980125123 56-57 23.91681150555361 25.940846058020096 26.110356042578903 24.031986393847394 58-59 24.057465857246328 25.880632900995348 26.048311343085206 24.013589898673118 60-61 24.112690076033395 25.81334132198213 25.918192337461534 24.15577626452294 62-63 23.998844671584767 25.979284054438885 25.984087976826594 24.037783297149755 64-65 24.188769435965376 25.874398383954123 25.850945428613205 24.0858867514673 66-67 24.12344266633963 25.91491534233814 25.843659858306623 24.11798213301561 68-69 24.06939264871665 25.995805814210936 25.868661351723777 24.066140185348637 70-71 24.27825332425838 25.88113025530913 25.711228523200656 24.12938789723183 72-73 24.062626300013314 25.93325449128071 25.87516767865874 24.128951530047242 74-75 24.03064144099072 25.949224068373088 25.923104454871726 24.097030035764465 76-77 24.211874861040386 26.072588916222593 25.699250753669133 24.01628546906789 78-79 24.272468933053894 26.10247079492235 25.527947122935835 24.097113149087924 80-81 24.039414546951367 26.086731837818107 25.710264187510347 24.163589427720183 82-83 24.268181696112865 26.087679146363662 25.50065496775888 24.1434841897646 84-85 24.172747137456685 25.999887208050122 25.58601833376451 24.241347320728686 86-87 24.093874338082014 26.158986836537814 25.587885819184862 24.159253006195307 88-89 24.297700882926126 26.127290679905933 25.406543184126008 24.168465253041933 90-91 24.293030432356126 26.106616209698842 25.245336063103046 24.35501729484199 92-93 24.09872212223856 26.168339200555756 25.4126178753682 24.320320801837482 94-95 24.309814829569852 26.204819366992925 25.12100043538969 24.36436536804753 96-97 24.30916442040474 26.18519224749435 25.10358467892703 24.402058653173878 98-99 24.187377016599516 26.219359713428158 25.165186439403385 24.428076830568937 100-101 24.356495976756985 26.085875737236453 24.967562192855446 24.590066093151115 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 3.0 4 8.5 5 13.5 6 20.5 7 27.5 8 43.0 9 65.5 10 90.0 11 154.0 12 255.0 13 357.0 14 498.0 15 712.0 16 1028.0 17 1406.0 18 1970.0 19 2763.5 20 3721.5 21 4899.5 22 6411.0 23 8196.0 24 10303.0 25 12694.0 26 15397.0 27 18343.5 28 21306.5 29 24485.5 30 27533.0 31 30376.5 32 33331.0 33 36342.5 34 38982.5 35 41240.0 36 43634.0 37 45819.0 38 46857.0 39 47299.0 40 47314.5 41 46558.0 42 45420.5 43 43881.5 44 42302.0 45 40851.5 46 39201.5 47 37629.0 48 36080.0 49 34204.5 50 32200.0 51 30288.0 52 28955.0 53 28136.5 54 27716.0 55 27486.5 56 27267.5 57 27411.5 58 27560.5 59 27966.5 60 28577.0 61 28669.0 62 29009.5 63 29380.0 64 29334.0 65 29339.0 66 29272.5 67 28825.5 68 28052.0 69 27102.5 70 25614.5 71 23851.0 72 21818.5 73 19143.5 74 16485.5 75 13950.0 76 11288.5 77 8763.0 78 6646.0 79 4892.0 80 3419.5 81 2364.0 82 1602.0 83 1075.5 84 719.5 85 469.5 86 322.5 87 227.0 88 159.0 89 122.0 90 85.0 91 51.5 92 38.5 93 24.0 94 15.5 95 9.0 96 3.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6972437864801303 2 0.002924079556446969 3 4.7740074390970917E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.18785719272847057 14-15 5.9675092988713647E-5 16-17 2.9837546494356827E-4 18-19 0.11135372351693967 20-21 0.0 22-23 2.9837546494356823E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0018797654291444798 42-43 0.0 44-45 0.0 46-47 0.023691011916519317 48-49 0.0 50-51 8.951263948307048E-5 52-53 1.1935018597742729E-4 54-55 0.0019394405221331936 56-57 0.001700740150178339 58-59 0.10165652090627371 60-61 0.0017902527896614096 62-63 0.0016112275106952684 64-65 0.002745054277480828 66-67 0.0048038449855914486 68-69 0.005251408183006801 70-71 0.003968393683749458 72-73 0.039504911558528436 74-75 0.045024857659984444 76-77 0.1547375161197345 78-79 2.9837546494356827E-4 80-81 0.11290527593464622 82-83 0.005132057997029374 84-85 0.005340920822489872 86-87 0.007041660972668211 88-89 0.00713117361215128 90-91 0.023034585893643468 92-93 0.011308430121361237 94-95 0.013755108933898497 96-97 0.0107116791914741 98-99 0.012531769527629867 100-101 0.013546246108437998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1675741.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.63004137020795 #Duplication Level Percentage of deduplicated Percentage of total 1 98.88044898188359 96.53702324806031 2 0.5517199514091715 1.0772888336169306 3 0.21006926798731815 0.6152721397263351 4 0.1740436166239148 0.6796754196485365 5 0.08787575080699582 0.42896565933625436 6 0.0474970396813915 0.27822827694339963 7 0.025969240884370755 0.17747646433258085 8 0.011388607624458383 0.088949618681995 9 0.005798458435185446 0.05094933632195277 >10 0.004998900692612175 0.05113119147684297 >50 1.9018397099971774E-4 0.015039811854888825 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.7902527896614096E-4 0.0 5.9675092988713647E-5 2 2.9837546494356827E-4 0.0 5.9675092988713647E-5 3 2.9837546494356827E-4 0.0 5.9675092988713647E-5 4 3.580505579322819E-4 0.0 1.1935018597742729E-4 5 6.564260228758502E-4 0.0 1.1935018597742729E-4 6 7.161011158645638E-4 0.0 1.1935018597742729E-4 7 7.757762088532775E-4 0.0 1.1935018597742729E-4 8 8.951263948307047E-4 0.0 1.1935018597742729E-4 9 9.548014878194183E-4 5.9675092988713647E-5 1.1935018597742729E-4 10-11 0.0011039892202912025 5.9675092988713647E-5 1.1935018597742729E-4 12-13 0.0013128520457517004 5.9675092988713647E-5 1.1935018597742729E-4 14-15 0.001551552417706555 5.9675092988713647E-5 1.1935018597742729E-4 16-17 0.0016709026036839823 5.9675092988713647E-5 1.1935018597742729E-4 18-19 0.002088628254604978 1.1935018597742729E-4 1.1935018597742729E-4 20-21 0.0022676535335711184 1.1935018597742729E-4 1.1935018597742729E-4 22-23 0.002387003719548546 1.1935018597742729E-4 1.1935018597742729E-4 24-25 0.0026555416379977575 1.1935018597742729E-4 1.1935018597742729E-4 26-27 0.0029837546494356827 1.1935018597742729E-4 1.1935018597742729E-4 28-29 0.003819205951277674 1.1935018597742729E-4 1.1935018597742729E-4 30-31 0.005609458740939084 1.1935018597742729E-4 1.1935018597742729E-4 32-33 0.00883191376232962 1.1935018597742729E-4 1.1935018597742729E-4 34-35 0.01447121004976306 1.1935018597742729E-4 1.1935018597742729E-4 36-37 0.022437834963756335 1.1935018597742729E-4 1.1935018597742729E-4 38-39 0.03279146359729815 1.1935018597742729E-4 1.1935018597742729E-4 40-41 0.04815780004189192 1.1935018597742729E-4 1.1935018597742729E-4 42-43 0.06808928110012227 1.1935018597742729E-4 1.7902527896614096E-4 44-45 0.09625592499079512 1.1935018597742729E-4 1.7902527896614096E-4 46-47 0.13444798450357184 1.1935018597742729E-4 1.7902527896614096E-4 48-49 0.17881641614068045 1.1935018597742729E-4 1.7902527896614096E-4 50-51 0.2365520686072609 1.1935018597742729E-4 2.3870037195485459E-4 52-53 0.309057306588548 1.1935018597742729E-4 2.3870037195485459E-4 54-55 0.39024527059969294 1.1935018597742729E-4 2.3870037195485459E-4 56-57 0.4922300045174045 1.1935018597742729E-4 2.3870037195485459E-4 58-59 0.6102673384490802 1.1935018597742729E-4 2.3870037195485459E-4 60-61 0.7478184277880651 1.1935018597742729E-4 2.9837546494356827E-4 62-63 0.8965585970624339 1.1935018597742729E-4 2.9837546494356827E-4 64-65 1.0742113488898344 1.1935018597742729E-4 2.9837546494356827E-4 66-67 1.2710794806596009 1.1935018597742729E-4 2.9837546494356827E-4 68-69 1.4916684618923808 1.1935018597742729E-4 2.9837546494356827E-4 70-71 1.7366347186110502 1.1935018597742729E-4 2.9837546494356827E-4 72-73 1.9978922757156385 1.1935018597742729E-4 2.9837546494356827E-4 74-75 2.2787829384135136 1.1935018597742729E-4 2.9837546494356827E-4 76-77 2.5896603353382175 1.1935018597742729E-4 2.9837546494356827E-4 78-79 2.9366710010675874 1.1935018597742729E-4 2.9837546494356827E-4 80-81 3.314474014779133 1.7902527896614096E-4 3.580505579322819E-4 82-83 3.71152224597954 1.7902527896614096E-4 3.580505579322819E-4 84-85 4.132380839282443 1.7902527896614096E-4 3.580505579322819E-4 86-87 4.581972989859411 1.7902527896614096E-4 3.580505579322819E-4 88-89 5.063222777266892 1.7902527896614096E-4 3.580505579322819E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACGT 2715 0.0 7.342308 94-95 AATCTCG 1245 0.0 7.243316 94-95 AGTCACG 2670 0.0 6.9327655 94-95 CGTATGC 800 3.472269E-8 6.526132 94-95 CGTGAAA 2100 0.0 6.3283706 94-95 CTCGTAT 835 8.079223E-8 6.2525816 94-95 CAGTCAC 3240 0.0 6.079519 92-93 CACGTGA 2200 0.0 5.9330244 92-93 TCTCGTA 885 2.5189547E-7 5.8993287 94-95 TCACGTG 2360 0.0 5.8322906 94-95 TCCAGTC 3930 0.0 5.7365956 94-95 TCGTATG 835 3.3347733E-6 5.686117 90-91 CCAGTCA 3440 0.0 5.656901 94-95 ACGTCTG 6355 0.0 5.638772 94-95 TATGCCG 815 1.34667425E-5 5.5326366 92-93 CTCCAGT 4025 0.0 5.4243183 94-95 AACTCCA 4815 0.0 5.4215016 94-95 ACGTGAA 2255 0.0 5.3671885 94-95 CGTCTGA 5730 0.0 5.301253 94-95 GTATGCC 985 1.9784075E-6 5.30057 92-93 >>END_MODULE