FastQCFastQC Report
Thu 11 Sep 2014
NYCLib19_GTGAAA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib19_GTGAAA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675741
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT27150.07.34230894-95
AATCTCG12450.07.24331694-95
AGTCACG26700.06.932765594-95
CGTATGC8003.472269E-86.52613294-95
CGTGAAA21000.06.328370694-95
CTCGTAT8358.079223E-86.252581694-95
CAGTCAC32400.06.07951992-93
CACGTGA22000.05.933024492-93
TCTCGTA8852.5189547E-75.899328794-95
TCACGTG23600.05.832290694-95
TCCAGTC39300.05.736595694-95
TCGTATG8353.3347733E-65.68611790-91
CCAGTCA34400.05.65690194-95
ACGTCTG63550.05.63877294-95
TATGCCG8151.34667425E-55.532636692-93
CTCCAGT40250.05.424318394-95
AACTCCA48150.05.421501694-95
ACGTGAA22550.05.367188594-95
CGTCTGA57300.05.30125394-95
GTATGCC9851.9784075E-65.3005792-93
Produced by FastQC (version 0.11.2)