##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib18_GTCCGC_L007_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1847981
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.968977494898486	33.0	22.0	33.0	2.0	33.0
2	25.02459494983985	33.0	15.0	33.0	2.0	33.0
3	25.90993089214662	33.0	22.0	33.0	6.0	33.0
4	28.02895105523271	33.0	22.0	37.0	2.0	37.0
5	28.0903245217348	33.0	22.0	37.0	6.0	37.0
6	29.160744618045314	33.0	27.0	37.0	15.0	37.0
7	28.824648088914333	33.0	27.0	37.0	6.0	37.0
8	28.58791730001553	33.0	27.0	37.0	2.0	37.0
9	29.363197457116712	33.0	27.0	37.0	15.0	37.0
10-11	28.990216890758077	33.0	27.0	37.0	10.5	37.0
12-13	29.293537379442753	33.0	27.0	37.0	10.5	37.0
14-15	29.635231639286335	33.0	27.0	37.0	6.0	40.0
16-17	30.525251071304304	33.0	27.0	37.0	15.0	40.0
18-19	30.837333554836334	33.0	27.0	37.0	15.0	40.0
20-21	30.859926860719888	33.0	27.0	37.0	15.0	40.0
22-23	30.8914496415277	33.0	27.0	37.0	15.0	40.0
24-25	30.46841201289407	33.0	27.0	37.0	10.5	40.0
26-27	29.951543062401615	33.0	27.0	37.0	15.0	40.0
28-29	30.14528287899064	33.0	27.0	37.0	15.0	40.0
30-31	30.055051161240293	33.0	27.0	37.0	15.0	40.0
32-33	29.893107937798064	33.0	27.0	37.0	15.0	40.0
34-35	29.942689075266465	33.0	27.0	37.0	15.0	40.0
36-37	30.180183400154007	33.0	27.0	37.0	15.0	40.0
38-39	30.227467706648497	33.0	27.0	37.0	15.0	40.0
40-41	30.306694982253603	33.0	27.0	37.0	15.0	40.0
42-43	30.220798265783035	33.0	27.0	37.0	15.0	40.0
44-45	29.95844356624879	33.0	27.0	37.0	15.0	40.0
46-47	29.583531432411913	33.0	27.0	37.0	15.0	40.0
48-49	29.423152077862273	33.0	24.5	37.0	10.5	40.0
50-51	28.658017587843165	33.0	24.5	37.0	10.5	38.5
52-53	28.370379890269433	33.0	22.0	37.0	10.5	37.0
54-55	29.308264262457243	33.0	24.5	37.0	15.0	40.0
56-57	29.23515663851522	33.0	24.5	37.0	10.5	40.0
58-59	29.443609268710013	33.0	27.0	37.0	15.0	40.0
60-61	29.198625689333387	33.0	24.5	37.0	10.5	40.0
62-63	29.062822615600485	33.0	24.5	37.0	15.0	40.0
64-65	29.020908494189065	33.0	24.5	37.0	15.0	40.0
66-67	28.89014713895868	33.0	22.0	37.0	15.0	40.0
68-69	28.615766341753513	33.0	22.0	37.0	15.0	40.0
70-71	28.51725748262563	33.0	22.0	37.0	15.0	38.5
72-73	28.25507215712716	33.0	22.0	37.0	15.0	37.0
74-75	28.200136256812165	33.0	22.0	37.0	15.0	37.0
76-77	27.79659531131543	33.0	22.0	37.0	6.0	37.0
78-79	27.19984972789222	33.0	22.0	35.0	6.0	37.0
80-81	27.060343152878737	27.0	22.0	33.0	6.0	37.0
82-83	26.611315808982884	27.0	22.0	33.0	6.0	37.0
84-85	26.68710717263868	27.0	22.0	33.0	6.0	37.0
86-87	26.368115527161805	27.0	22.0	33.0	6.0	37.0
88-89	25.972593062374557	27.0	22.0	33.0	6.0	37.0
90-91	25.814924504093927	27.0	22.0	33.0	6.0	37.0
92-93	25.668124834616805	27.0	22.0	33.0	6.0	37.0
94-95	25.417703158203466	27.0	22.0	33.0	6.0	37.0
96-97	25.24923416420407	27.0	18.5	33.0	6.0	37.0
98-99	24.976771676765075	27.0	15.0	33.0	6.0	37.0
100-101	24.45151465301862	27.0	15.0	33.0	6.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	8.432750115821968
2306	2	8.52163283688921
2306	3	7.529754739216351
2306	4	9.140990597579975
2306	5	8.852412684548039
2306	6	7.931192782254939
2306	7	8.2077895828951
2306	8	8.636763796790312
2306	9	7.459453838494319
2306	10-11	7.79663709025515
2306	12-13	7.4498169432045955
2306	14-15	8.14400225778084
2306	16-17	6.863140088883583
2306	18-19	6.155314285707764
2306	20-21	6.156185451724692
2306	22-23	5.817384249661902
2306	24-25	5.844290467337586
2306	26-27	6.52601937685197
2306	28-29	5.541328382623767
2306	30-31	5.420878104182979
2306	32-33	5.3358271023288495
2306	34-35	4.721230642929111
2306	36-37	4.709669900383709
2306	38-39	4.5269275823630295
2306	40-41	3.687098066542241
2306	42-43	3.3715324900780566
2306	44-45	3.076719468625445
2306	46-47	3.341751856967246
2306	48-49	3.285178747747885
2306	50-51	3.150812776954222
2306	52-53	3.7402942695949655
2306	54-55	3.3964843930547346
2306	56-57	3.617100611165988
2306	58-59	3.1258096922931067
2306	60-61	3.1085824309028602
2306	62-63	2.6327815856197603
2306	64-65	2.291587109508473
2306	66-67	2.138258864633457
2306	68-69	2.3587547021849993
2306	70-71	2.394160201073735
2306	72-73	1.5709169884003273
2306	74-75	1.2315613377021783
2306	76-77	1.4447423093355525
2306	78-79	1.8110715881021626
2306	80-81	1.6882596347697891
2306	82-83	1.5768148908185502
2306	84-85	1.048436408607678
2306	86-87	1.007275776908191
2306	88-89	1.0040642304231433
2306	90-91	0.9606144185022174
2306	92-93	0.9063259821864591
2306	94-95	1.015549644518849
2306	96-97	0.7226755004411345
2306	98-99	0.5696959144513869
2306	100-101	0.5053517317258951
2307	1	8.280301793492974
2307	2	8.316829782496342
2307	3	7.501713856524926
2307	4	9.136785554806853
2307	5	9.024040988767435
2307	6	7.52586547585156
2307	7	8.146592203670874
2307	8	8.59012319930271
2307	9	7.450377867586894
2307	10-11	7.7328658235220225
2307	12-13	7.256928549192189
2307	14-15	8.236902113092803
2307	16-17	6.952497030507196
2307	18-19	6.497228723816004
2307	20-21	6.35350712110581
2307	22-23	6.025966273327565
2307	24-25	6.213552553358674
2307	26-27	7.025315463072737
2307	28-29	6.210040419397274
2307	30-31	5.987780987860848
2307	32-33	6.054399393202978
2307	34-35	5.762994351909949
2307	36-37	5.688120235828507
2307	38-39	5.294712712922504
2307	40-41	4.660102422084414
2307	42-43	4.500943383006419
2307	44-45	4.431790652857082
2307	46-47	4.6527995128769355
2307	48-49	4.596893034065307
2307	50-51	4.308633940448729
2307	52-53	4.810483398421958
2307	54-55	4.53175467359457
2307	56-57	4.895189983970898
2307	58-59	4.324175420250782
2307	60-61	4.2692716200786265
2307	62-63	3.9305715105538326
2307	64-65	3.889641476300852
2307	66-67	3.6321966691401926
2307	68-69	3.834976323982481
2307	70-71	3.3739113567651167
2307	72-73	2.8166377824510818
2307	74-75	2.3710451980069465
2307	76-77	2.513271474431413
2307	78-79	2.929118161707546
2307	80-81	2.7025331435963587
2307	82-83	2.822734798561921
2307	84-85	2.0899405619938918
2307	86-87	2.0304336865459653
2307	88-89	2.3885263217092287
2307	90-91	2.084753424073675
2307	92-93	1.8550924210178863
2307	94-95	2.222964273359004
2307	96-97	1.8741175132191827
2307	98-99	1.7729725857522425
2307	100-101	1.3650238888889952
1101	1	8.104997824401973
1101	2	7.863028108509571
1101	3	7.345862682690118
1101	4	8.83897181063487
1101	5	8.633148835873623
1101	6	7.464900760095674
1101	7	7.12201007917589
1101	8	8.020224929186824
1101	9	7.447511998395797
1101	10-11	7.622000159433281
1101	12-13	7.314887415294805
1101	14-15	7.784393298401703
1101	16-17	7.039015672787755
1101	18-19	6.064723932772882
1101	20-21	5.895457349895462
1101	22-23	5.3976825254701915
1101	24-25	6.0458184355615145
1101	26-27	6.42936875099187
1101	28-29	6.0395140580857
1101	30-31	5.697443237260888
1101	32-33	5.835045520002382
1101	34-35	5.535841531117125
1101	36-37	5.35270166358243
1101	38-39	5.021750748471852
1101	40-41	4.522798090460455
1101	42-43	4.257991168504763
1101	44-45	4.187511216195617
1101	46-47	4.533776572709346
1101	48-49	4.389735385400709
1101	50-51	3.747905338981891
1101	52-53	4.825573279598526
1101	54-55	4.540436054479699
1101	56-57	4.8241714908068225
1101	58-59	4.3238169653139025
1101	60-61	4.413007307882488
1101	62-63	3.9798071407279814
1101	64-65	3.7774396120822793
1101	66-67	3.3458105846902484
1101	68-69	3.4797691677563805
1101	70-71	3.39683097617759
1101	72-73	3.030327663008041
1101	74-75	2.4373787082231466
1101	76-77	2.6903551047911876
1101	78-79	3.097213263447234
1101	80-81	2.8297629283533183
1101	82-83	2.8648257032902933
1101	84-85	2.094143453241653
1101	86-87	2.277403479407127
1101	88-89	2.3044468105306137
1101	90-91	2.2876950622737517
1101	92-93	2.0802689503534353
1101	94-95	2.1346055581874452
1101	96-97	1.9850020500125112
1101	98-99	1.8789808203210576
1101	100-101	1.5837004311540852
2201	1	7.304945463921342
2201	2	7.978250560356493
2201	3	6.47528048641653
2201	4	8.591143534096641
2201	5	4.124761797476083
2201	6	5.781624859917471
2201	7	6.89339472505603
2201	8	8.023482027134342
2201	9	6.657033924970094
2201	10-11	4.360384986217152
2201	12-13	6.74293629342646
2201	14-15	7.318589344246071
2201	16-17	6.156527793369968
2201	18-19	1.8681457611121708
2201	20-21	5.270010769758851
2201	22-23	5.338727685138668
2201	24-25	5.578288483404833
2201	26-27	6.42892837724002
2201	28-29	5.245602328245333
2201	30-31	5.332513340174092
2201	32-33	5.4064998435345615
2201	34-35	5.019070681322628
2201	36-37	5.225954953669266
2201	38-39	4.63455141626288
2201	40-41	4.362594413686288
2201	42-43	4.265482794118764
2201	44-45	4.062199786677169
2201	46-47	4.447401013871126
2201	48-49	4.2850149992495155
2201	50-51	3.924016243515265
2201	52-53	4.702348888739618
2201	54-55	4.408816829301067
2201	56-57	4.533719712643244
2201	58-59	4.228412386666555
2201	60-61	4.1133802085668485
2201	62-63	3.8814828614210484
2201	64-65	3.7494404297594954
2201	66-67	3.3358401433765152
2201	68-69	3.4993986608035357
2201	70-71	3.3333129972793607
2201	72-73	2.688566064481037
2201	74-75	2.4419354247391993
2201	76-77	2.833038756429257
2201	78-79	2.9968631945907056
2201	80-81	2.6938421753081307
2201	82-83	2.828584058585868
2201	84-85	2.5351962490798066
2201	86-87	2.36439082643372
2201	88-89	2.3601757046528498
2201	90-91	2.3107376675635933
2201	92-93	2.0252188126691415
2201	94-95	2.273935174058714
2201	96-97	2.265683844304071
2201	98-99	2.0380380492272856
2201	100-101	1.7193226067697545
2304	1	1.9406083818948439
2304	2	5.136465960534647
2304	3	5.978598830764767
2304	4	4.564536253268052
2304	5	6.788044631049022
2304	6	6.46917474217971
2304	7	7.278019309030764
2304	8	6.63104901571139
2304	9	6.229888741248807
2304	10-11	6.933747823697505
2304	12-13	6.7647110240957105
2304	14-15	6.96118050632419
2304	16-17	5.593169399604577
2304	18-19	5.489363725209191
2304	20-21	5.201020857730125
2304	22-23	4.8732135338496825
2304	24-25	4.98950310721073
2304	26-27	6.038568545048072
2304	28-29	5.257809481790737
2304	30-31	5.280691607706178
2304	32-33	5.354275117964583
2304	34-35	5.1324884936501896
2304	36-37	5.1944371122389015
2304	38-39	4.758028478393669
2304	40-41	4.046739056973301
2304	42-43	3.7596834499581924
2304	44-45	3.502261560562424
2304	46-47	4.472372915102039
2304	48-49	4.170313361403441
2304	50-51	3.682095963128777
2304	52-53	4.39411161886332
2304	54-55	4.180091672462755
2304	56-57	4.940291274993218
2304	58-59	3.9346239245367514
2304	60-61	4.029090716561278
2304	62-63	3.7745838499346505
2304	64-65	3.5522336822902894
2304	66-67	3.2969133788334446
2304	68-69	3.563430954449281
2304	70-71	3.6244454127641923
2304	72-73	3.2900001665897705
2304	74-75	2.531997446083473
2304	76-77	2.8733209067718875
2304	78-79	3.245874960902089
2304	80-81	2.8582366272606343
2304	82-83	3.015820195262112
2304	84-85	2.5502727102665084
2304	86-87	2.5263509306845506
2304	88-89	2.708023983551012
2304	90-91	2.664128663446604
2304	92-93	2.4416308061397913
2304	94-95	2.7573369210220484
2304	96-97	2.6518857158569347
2304	98-99	2.490603927484102
2304	100-101	2.0965468678469783
1102	1	7.018252186938817
1102	2	7.529386213131382
1102	3	6.087072371109997
1102	4	6.264661899022329
1102	5	6.600818024413105
1102	6	5.969231666447669
1102	7	6.855485617932143
1102	8	6.445637262311266
1102	9	4.928469453406361
1102	10-11	5.9851822166409825
1102	12-13	5.923482309737921
1102	14-15	6.228598839273246
1102	16-17	5.095235660886786
1102	18-19	5.218864265407053
1102	20-21	5.39391017400423
1102	22-23	5.270833624333267
1102	24-25	1.8937590433809675
1102	26-27	5.4702474559604255
1102	28-29	5.045100793838635
1102	30-31	4.9558006064737405
1102	32-33	4.956369957918646
1102	34-35	4.950708060322942
1102	36-37	4.847028932427776
1102	38-39	3.7545954071689494
1102	40-41	3.9768325597897487
1102	42-43	4.11583483874897
1102	44-45	-4.095429223586844
1102	46-47	1.2552290646979358
1102	48-49	-0.6225787607440267
1102	50-51	2.5737149807467965
1102	52-53	-0.22709964529289195
1102	54-55	2.6136621274947345
1102	56-57	-0.8296374629948637
1102	58-59	2.8256050765978777
1102	60-61	3.6547542204498775
1102	62-63	3.4541750745253594
1102	64-65	3.235664874565945
1102	66-67	3.156450192788448
1102	68-69	3.417803302988599
1102	70-71	3.2664668894388704
1102	72-73	2.661521555950685
1102	74-75	2.529178305329747
1102	76-77	2.732349525963059
1102	78-79	-1.2965792029601992
1102	80-81	1.6633309368777383
1102	82-83	2.645394844581727
1102	84-85	2.2578223562961703
1102	86-87	2.2333891547477975
1102	88-89	2.2041386109184806
1102	90-91	2.1131006739185167
1102	92-93	1.8562309127091936
1102	94-95	2.2647035039846415
1102	96-97	2.287232889347404
1102	98-99	2.176626473797146
1102	100-101	1.883342759474644
2202	1	-20.73934061396107
2202	2	-7.752287601602616
2202	3	-0.488881044795459
2202	4	1.4405663752362443
2202	5	0.8460623924844057
2202	6	1.456582601325227
2202	7	3.937306676799267
2202	8	2.6353281249829763
2202	9	3.0971098728386437
2202	10-11	1.9800493935839505
2202	12-13	1.314492573355352
2202	14-15	2.6614173269227805
2202	16-17	1.6130954120575325
2202	18-19	1.6620225428999298
2202	20-21	1.0094951279375444
2202	22-23	1.2950438462949627
2202	24-25	2.361957759395125
2202	26-27	3.3231254788509865
2202	28-29	-1.169185306863909
2202	30-31	1.001126238004428
2202	32-33	1.4241367565209018
2202	34-35	1.5524328704860118
2202	36-37	2.8640764837236468
2202	38-39	2.3884936207253062
2202	40-41	1.8253418023007058
2202	42-43	0.6678216202020444
2202	44-45	0.5970516169006714
2202	46-47	-1.3839440767047329
2202	48-49	0.7067478808802541
2202	50-51	1.6332343303763572
2202	52-53	1.7361339042664206
2202	54-55	-0.7386168044027741
2202	56-57	1.022885442828791
2202	58-59	1.8981874886812875
2202	60-61	2.1111014839811304
2202	62-63	-2.0278576055188005
2202	64-65	0.0355829252166906
2202	66-67	0.6121669206570957
2202	68-69	1.5012854229022423
2202	70-71	-2.7160398274516773
2202	72-73	-4.691274788082403
2202	74-75	-1.2841922193354343
2202	76-77	-0.37048971033817324
2202	78-79	1.5607980642922534
2202	80-81	1.554370809684773
2202	82-83	-0.7473716352266422
2202	84-85	-0.25909535894476576
2202	86-87	0.6571505632216841
2202	88-89	1.2259823679102126
2202	90-91	1.3413795076661543
2202	92-93	1.414728189823002
2202	94-95	1.6927549604089975
2202	96-97	1.1658758823157775
2202	98-99	1.0696448007745687
2202	100-101	0.8375422699618049
2305	1	5.887822298216534
2305	2	7.308635128025834
2305	3	6.879604395449267
2305	4	7.858693974591727
2305	5	8.014423185912872
2305	6	6.350079108264744
2305	7	3.081482466269293
2305	8	6.126785713270081
2305	9	6.484130162321172
2305	10-11	4.974860860507853
2305	12-13	5.538843103889391
2305	14-15	5.878894954927738
2305	16-17	5.126014088926084
2305	18-19	4.595532595675753
2305	20-21	5.284628918025081
2305	22-23	5.333572767126515
2305	24-25	5.400438286218019
2305	26-27	6.0957100878963715
2305	28-29	5.194791191255902
2305	30-31	5.541243188883559
2305	32-33	5.524453835411428
2305	34-35	5.396488588981146
2305	36-37	4.6658309039247285
2305	38-39	4.676702428928351
2305	40-41	4.250969295391236
2305	42-43	3.9343483423866807
2305	44-45	3.2325365601955696
2305	46-47	3.8684943813129884
2305	48-49	3.6313607260072054
2305	50-51	2.3335244028305446
2305	52-53	4.038051114510516
2305	54-55	4.251725030013901
2305	56-57	4.706130394010277
2305	58-59	4.367988001063367
2305	60-61	4.489499228450221
2305	62-63	3.7293578963102476
2305	64-65	4.177845384935548
2305	66-67	3.6990048942636875
2305	68-69	3.9495539099462746
2305	70-71	3.8174234238877354
2305	72-73	-0.8812926013393998
2305	74-75	2.061561439122645
2305	76-77	2.9731070781838227
2305	78-79	3.5337352710753116
2305	80-81	3.166058574008307
2305	82-83	3.3842090947838024
2305	84-85	2.6823873684316517
2305	86-87	2.694323610663627
2305	88-89	2.867929229157806
2305	90-91	2.8665190455936553
2305	92-93	2.861259315133424
2305	94-95	2.901029034914238
2305	96-97	2.8671248066285564
2305	98-99	2.803999769201827
2305	100-101	2.2503838192295404
1103	1	-20.73934061396107
1103	2	-7.851750012641446
1103	3	-0.7483943670137059
1103	4	2.915450772926178
1103	5	4.775970312314286
1103	6	4.681585194246221
1103	7	4.169836114695961
1103	8	4.599975430773192
1103	9	4.617472725903056
1103	10-11	4.995960695901754
1103	12-13	4.708229275267755
1103	14-15	4.32340372622782
1103	16-17	3.1028533441475794
1103	18-19	3.416985573541961
1103	20-21	2.3534470872565656
1103	22-23	2.799593658255734
1103	24-25	3.240283218042382
1103	26-27	4.419360377709083
1103	28-29	3.7806159225103784
1103	30-31	3.646980472079548
1103	32-33	3.1474547601455924
1103	34-35	2.5540109851536705
1103	36-37	2.942926042147704
1103	38-39	2.487193802919222
1103	40-41	2.7309217083320476
1103	42-43	2.4001466819675237
1103	44-45	2.738634335876597
1103	46-47	2.092957103928633
1103	48-49	-1.0376194853654894
1103	50-51	-1.6353301746035491
1103	52-53	1.9887872268323825
1103	54-55	2.496583012384125
1103	56-57	3.4091377457323198
1103	58-59	2.928889351927335
1103	60-61	3.337707791044128
1103	62-63	3.015018876118223
1103	64-65	2.917257178800167
1103	66-67	2.647566257468842
1103	68-69	3.125111698994072
1103	70-71	3.1465855395290774
1103	72-73	2.396008508170663
1103	74-75	1.9432602492752373
1103	76-77	2.35397961016065
1103	78-79	3.093975462654246
1103	80-81	2.6068265306876732
1103	82-83	2.665789791941055
1103	84-85	2.133030494545906
1103	86-87	1.9404733150392772
1103	88-89	2.0943115016108074
1103	90-91	-2.565918440838736
1103	92-93	-3.115448494511501
1103	94-95	-2.8093907191851066
1103	96-97	0.36331647444900383
1103	98-99	-1.0495481503172464
1103	100-101	0.9235036261464167
2203	1	2.646902772282317
2203	2	3.2059073027570832
2203	3	-5.87584744248473
2203	4	-3.2528091313845877
2203	5	0.10714015081396866
2203	6	0.1523382424113855
2203	7	2.5272468858688875
2203	8	-7.866418968755088
2203	9	-6.633127013701916
2203	10-11	-12.198137874300034
2203	12-13	-10.105708981826822
2203	14-15	-1.292310005454496
2203	16-17	-1.2671260379484792
2203	18-19	-0.5792948932695445
2203	20-21	-1.2086238842384915
2203	22-23	-3.020667884406709
2203	24-25	-0.8110092593907083
2203	26-27	-1.4310691949344019
2203	28-29	0.6068534914181036
2203	30-31	-2.1091823420862603
2203	32-33	0.027994189413519166
2203	34-35	-0.08405991510870336
2203	36-37	-0.28452307595212645
2203	38-39	0.46392861551790787
2203	40-41	-0.7037430825406688
2203	42-43	0.3623769376532735
2203	44-45	0.042984278296849254
2203	46-47	0.44028033805067324
2203	48-49	0.3730586893366201
2203	50-51	0.24845429904629057
2203	52-53	1.031840457432363
2203	54-55	0.4002111302979543
2203	56-57	0.3436600712419313
2203	58-59	0.6669329463284477
2203	60-61	0.30719052305490635
2203	62-63	0.8097347962268131
2203	64-65	0.27061065515289684
2203	66-67	-0.4506673698971717
2203	68-69	0.45913816589263945
2203	70-71	0.5363036955523945
2203	72-73	0.1288857544734867
2203	74-75	-0.40153422465162336
2203	76-77	-0.23447456150219637
2203	78-79	0.4747626233591298
2203	80-81	0.3770415742385431
2203	82-83	-0.15377683633911232
2203	84-85	-0.08704524953881432
2203	86-87	-0.13336410823718836
2203	88-89	0.1914526966577661
2203	90-91	-0.38433268262620146
2203	92-93	-0.05755888142368093
2203	94-95	0.32097102411605505
2203	96-97	-0.04622989209062922
2203	98-99	-0.2846082825725915
2203	100-101	-0.4456332316217093
2310	1	-20.73934061396107
2310	2	-20.55750917494511
2310	3	-21.477888258811262
2310	4	-23.46255969827801
2310	5	-23.636246092572275
2310	6	-24.70933976212376
2310	7	-24.26262461828833
2310	8	-23.901944289994695
2310	9	-24.83418521476012
2310	10-11	-24.565295848600865
2310	12-13	-24.838891143580412
2310	14-15	-25.166648629793123
2310	16-17	-26.205523619203205
2310	18-19	-20.354491007751374
2310	20-21	-20.437500488115095
2310	22-23	-26.803547703000813
2310	24-25	-26.418530045482925
2310	26-27	-19.327856520293157
2310	28-29	-19.813719679155067
2310	30-31	-20.245738889357092
2310	32-33	-18.472913490065586
2310	34-35	-10.725564592327668
2310	36-37	-15.360748420034614
2310	38-39	-20.223705447116153
2310	40-41	-12.305385541325986
2310	42-43	-5.506881931605594
2310	44-45	-14.484723160124876
2310	46-47	-19.79300860952399
2310	48-49	-20.26834616635395
2310	50-51	-11.952521259072121
2310	52-53	-15.547538782661164
2310	54-55	-19.59575932995822
2310	56-57	-19.01968100638486
2310	58-59	-11.614314442061797
2310	60-61	-8.445471926036113
2310	62-63	-5.307052445560426
2310	64-65	-15.949590761477092
2310	66-67	-11.57184513393208
2310	68-69	-6.13084513837638
2310	70-71	-3.942825015719176
2310	72-73	-4.550150656705782
2310	74-75	-4.395336236310779
2310	76-77	-3.4406784462775093
2310	78-79	-4.009809843356191
2310	80-81	-3.463870070244532
2310	82-83	-13.183967509386926
2310	84-85	-11.119062888699311
2310	86-87	-6.780164433608945
2310	88-89	-4.903078522873454
2310	90-91	-2.356312547463208
2310	92-93	-1.8618622571556287
2310	94-95	-2.5856217931910486
2310	96-97	-1.5564075094111018
2310	98-99	-1.6159637567852094
2310	100-101	-1.5434757401785006
2204	1	-20.73934061396107
2204	2	-20.55750917494511
2204	3	-21.477888258811262
2204	4	-23.46255969827801
2204	5	-23.636246092572275
2204	6	-24.70933976212376
2204	7	-24.26262461828833
2204	8	-23.901944289994695
2204	9	-24.83418521476012
2204	10-11	-18.509466113869216
2204	12-13	-19.019026862272575
2204	14-15	-19.025377809311937
2204	16-17	-20.271840707420356
2204	18-19	-26.641653169913535
2204	20-21	-14.228604691696166
2204	22-23	-6.74000667894159
2204	24-25	-16.095272796870958
2204	26-27	-25.52933072176736
2204	28-29	-13.274677440236207
2204	30-31	-15.869104550739408
2204	32-33	-19.538690219630105
2204	34-35	-19.80477450158076
2204	36-37	-26.10122753551373
2204	38-39	-13.718980719303826
2204	40-41	-7.620618880772774
2204	42-43	-4.942770613175956
2204	44-45	-14.421194072459453
2204	46-47	-20.618860413321507
2204	48-49	-19.076305126379538
2204	50-51	-11.176264982044717
2204	52-53	-3.3104317931636764
2204	54-55	-25.670083654282543
2204	56-57	-19.382331764933213
2204	58-59	-12.089964198710938
2204	60-61	-15.997447721303086
2204	62-63	-12.683450708834076
2204	64-65	-6.0402855656974985
2204	66-67	-4.037705152999994
2204	68-69	-7.402955886890581
2204	70-71	-7.737685583482271
2204	72-73	-3.8986082851603214
2204	74-75	-3.5609912146585394
2204	76-77	-3.6801088643013884
2204	78-79	-18.04705688654093
2204	80-81	-7.78272013701255
2204	82-83	-4.471526604009565
2204	84-85	-3.047694242839608
2204	86-87	-2.3926389882488266
2204	88-89	-3.497427379329036
2204	90-91	-2.797411938593445
2204	92-93	-2.3178241637652874
2204	94-95	-1.8166207594614505
2204	96-97	-1.8924776241672134
2204	98-99	-2.1656134709938364
2204	100-101	-2.4577896741562206
2311	1	8.378148394343768
2311	2	8.661836208542923
2311	3	7.786156685008962
2311	4	9.18497327680748
2311	5	8.986128113583074
2311	6	8.044934785995437
2311	7	8.41226546476003
2311	8	8.479589662130365
2311	9	7.727856797941392
2311	10-11	8.025813091311203
2311	12-13	7.586731719145526
2311	14-15	8.351426602253774
2311	16-17	7.168316146307298
2311	18-19	6.389978486168534
2311	20-21	6.4236157075760865
2311	22-23	5.983701930609346
2311	24-25	6.029686857301641
2311	26-27	6.669741090641047
2311	28-29	5.752146771298591
2311	30-31	5.372488750060096
2311	32-33	5.449725140926731
2311	34-35	5.168359697908297
2311	36-37	5.155611741476989
2311	38-39	4.630050543852832
2311	40-41	3.7414892523734338
2311	42-43	3.6657684022562655
2311	44-45	3.443663830365189
2311	46-47	3.6102797767883352
2311	48-49	3.570820397287452
2311	50-51	3.3120131047538877
2311	52-53	3.879683622272527
2311	54-55	3.5132333950579557
2311	56-57	3.7108328156042916
2311	58-59	3.169678793079459
2311	60-61	3.1762677745384664
2311	62-63	2.854443403221694
2311	64-65	2.526371994590697
2311	66-67	2.0913315769974403
2311	68-69	2.1405519081794395
2311	70-71	1.9534694056729514
2311	72-73	1.4158499566446139
2311	74-75	1.067061393588073
2311	76-77	1.2673193547833108
2311	78-79	1.483553579355057
2311	80-81	1.3478681179592193
2311	82-83	1.3264030334701822
2311	84-85	0.8986450532880639
2311	86-87	0.6970743853494668
2311	88-89	0.6973052412255036
2311	90-91	0.3369504406214716
2311	92-93	0.41072278069565016
2311	94-95	0.6566328576493348
2311	96-97	0.8067187291425881
2311	98-99	0.3926576571854987
2311	100-101	0.2429266356840607
2205	1	8.531533172446697
2205	2	8.7687044172879
2205	3	7.747111741188739
2205	4	9.458071369683157
2205	5	9.31642381034035
2205	6	7.9702718883616726
2205	7	8.424996740934969
2205	8	8.716259593500453
2205	9	7.884746824074831
2205	10-11	7.955456578583604
2205	12-13	7.707953516613763
2205	14-15	8.220244574090373
2205	16-17	7.215107448757962
2205	18-19	6.522060422319473
2205	20-21	6.558676547260969
2205	22-23	6.1174474426302545
2205	24-25	6.012901993352024
2205	26-27	6.761810054931665
2205	28-29	6.029280547461667
2205	30-31	5.831897617405627
2205	32-33	5.760016604636348
2205	34-35	5.519250357220297
2205	36-37	5.693311299437724
2205	38-39	4.944370517197676
2205	40-41	4.5430597486241595
2205	42-43	4.3450347688547915
2205	44-45	4.350201161814013
2205	46-47	4.555961178887539
2205	48-49	4.154523934550131
2205	50-51	3.96146725503775
2205	52-53	4.7520357146075085
2205	54-55	4.310741588435903
2205	56-57	4.478874610131921
2205	58-59	4.081937734651543
2205	60-61	4.083771770440592
2205	62-63	3.6731427430085475
2205	64-65	3.392835170633308
2205	66-67	3.064231653358302
2205	68-69	3.0514563930756395
2205	70-71	2.875489790702428
2205	72-73	2.4852945855355806
2205	74-75	1.9945939299543376
2205	76-77	2.306420561379749
2205	78-79	2.5734121694774714
2205	80-81	2.1797515633042863
2205	82-83	2.2368946480043554
2205	84-85	1.7279922865112063
2205	86-87	1.5994147152663238
2205	88-89	1.700067275175254
2205	90-91	1.4168360534766542
2205	92-93	1.1751943493184527
2205	94-95	1.4410673959981395
2205	96-97	1.2509948068504357
2205	98-99	1.1851669414328718
2205	100-101	0.7281572122796938
2206	1	8.230790217932292
2206	2	8.392379741371848
2206	3	7.403375626649119
2206	4	8.894636055856768
2206	5	8.904114312266035
2206	6	7.6903146441956665
2206	7	8.185659755940254
2206	8	8.810719249872001
2206	9	7.512642728957481
2206	10-11	7.7946152844527
2206	12-13	7.55199999441021
2206	14-15	8.296817181117767
2206	16-17	7.145871098150536
2206	18-19	6.515715381061529
2206	20-21	6.447414405347072
2206	22-23	6.01945896202017
2206	24-25	6.243281852813794
2206	26-27	6.92747006816105
2206	28-29	6.022915434448699
2206	30-31	5.790026917854039
2206	32-33	6.080341173902475
2206	34-35	5.608053703151899
2206	36-37	5.573297273175484
2206	38-39	5.130625648976235
2206	40-41	4.622114842296341
2206	42-43	4.091491115483823
2206	44-45	4.120484808666156
2206	46-47	4.632074801797383
2206	48-49	4.42346439153906
2206	50-51	4.491828181142537
2206	52-53	5.026690090969094
2206	54-55	4.437667518267446
2206	56-57	4.677068272010924
2206	58-59	4.236373682385889
2206	60-61	4.397386891242601
2206	62-63	3.8598573082723107
2206	64-65	3.652128063674219
2206	66-67	3.3183222770326637
2206	68-69	3.5691323294979043
2206	70-71	3.283832930236475
2206	72-73	2.6182946478478932
2206	74-75	2.3975100563812326
2206	76-77	2.505658703466203
2206	78-79	3.020449997845393
2206	80-81	2.6631704063084136
2206	82-83	2.607722988363804
2206	84-85	2.1198757481040538
2206	86-87	1.9824302058679493
2206	88-89	2.136220400497301
2206	90-91	1.8553311332004654
2206	92-93	1.550851684316644
2206	94-95	1.7503521405056688
2206	96-97	1.5762537924059608
2206	98-99	1.5068749938614907
2206	100-101	1.2531809988072915
2309	1	8.152158858055827
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2309	14-15	7.337047251938664
2309	16-17	6.672776275200171
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2309	20-21	6.24079240110704
2309	22-23	5.717043638076273
2309	24-25	5.877008497283711
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2309	28-29	5.645269849396236
2309	30-31	5.512618032410604
2309	32-33	5.648768129636089
2309	34-35	5.351083765735691
2309	36-37	5.41012410123389
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2207	2	7.418243556203304
2207	3	6.537033137405096
2207	4	7.500403264684952
2207	5	7.194822385978217
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2207	8	6.851053311923771
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2207	76-77	1.9913189533457896
2207	78-79	2.6388177737966068
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2207	82-83	2.043307672924225
2207	84-85	1.7934367594118505
2207	86-87	1.8131766183652047
2207	88-89	1.9128565259752612
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2207	96-97	1.6807748986346134
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1113	2	6.579790021842037
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1113	4	7.061285683248091
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2104	22-23	-2.617539279977887
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2104	34-35	-4.003308772037219
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2110	14-15	-25.166648629793123
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2110	32-33	-19.94125172958521
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2110	36-37	-26.10122753551373
2110	38-39	-15.658040737060034
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2110	46-47	-3.9838646315255986
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2110	76-77	-2.8290519575627187
2110	78-79	-2.1872205204685393
2110	80-81	-2.109447465821926
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2110	86-87	-2.2893342130651178
2110	88-89	-2.1584829020845184
2110	90-91	-2.1776506462476064
2110	92-93	-1.9266437070802773
2110	94-95	-1.7621843939779929
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2109	1	7.75641656619559
2109	2	7.876237561347317
2109	3	6.98359999184148
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2109	12-13	6.272074913860841
2109	14-15	7.191706461590691
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2109	72-73	1.6166404371414487
2109	74-75	0.9641783934388393
2109	76-77	1.2958280875527848
2109	78-79	2.0497075128321818
2109	80-81	1.6346778605488304
2109	82-83	1.7650677322396966
2109	84-85	1.1422065251484312
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2108	2	7.3339721055757785
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2211	1	6.7319431698227135
2211	2	6.285396230460297
2211	3	5.720028407855406
2211	4	7.775615977397667
2211	5	7.525353006526824
2211	6	7.2332278054438035
2211	7	7.074931688017973
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2211	9	5.819812532987626
2211	10-11	6.011280727975713
2211	12-13	5.73247709966283
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2211	34-35	4.609867430604357
2211	36-37	4.853023590612395
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2211	40-41	3.9043554496650046
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2211	46-47	4.308982586217208
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2211	54-55	3.583576255627367
2211	56-57	3.948390758561903
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2211	60-61	0.7732908708516817
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2211	64-65	2.898254242617913
2211	66-67	3.06645083248889
2211	68-69	3.275891143359903
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2211	72-73	2.701781673351551
2211	74-75	2.104036716187178
2211	76-77	2.351372127023062
2211	78-79	2.610775230353884
2211	80-81	2.639989471115012
2211	82-83	2.8511215569521404
2211	84-85	2.241706972070549
2211	86-87	2.2262340102895024
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2211	92-93	2.0480604496420796
2211	94-95	2.3528635671693117
2211	96-97	2.041719026215432
2211	98-99	1.8448521727807226
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2210	1	7.3651709082719705
2210	2	7.586275310611526
2210	3	6.810978992081306
2210	4	8.51764153508778
2210	5	8.51435436182565
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2210	7	7.838965774441302
2210	8	7.562190731102348
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2210	10-11	7.237041055001857
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2210	14-15	4.9352663328813335
2210	16-17	1.5960018595374628
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2210	30-31	5.264357369156887
2210	32-33	5.535329948557596
2210	34-35	5.209851969671174
2210	36-37	5.268295346667379
2210	38-39	4.416012045449442
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2210	44-45	4.480715423984591
2210	46-47	4.476824448283747
2210	48-49	4.208597405526323
2210	50-51	4.465130680731718
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2210	54-55	4.398400604075135
2210	56-57	4.612444280803718
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2210	60-61	4.157962315056356
2210	62-63	3.708027197683773
2210	64-65	3.667933762693
2210	66-67	3.294439425390454
2210	68-69	3.3812327312964285
2210	70-71	3.5468781622751386
2210	72-73	3.1310420102463823
2210	74-75	2.665952956307528
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2210	78-79	3.4457447087173456
2210	80-81	3.1864386101274427
2210	82-83	2.998982081790512
2210	84-85	2.486654207524115
2210	86-87	2.576203198075145
2210	88-89	2.7921543859670486
2210	90-91	2.6120247373297723
2210	92-93	2.4119591432480476
2210	94-95	2.6299792752581226
2210	96-97	2.416899403769225
2210	98-99	2.4768455037455688
2210	100-101	2.271982820533225
2209	1	5.252492120439452
2209	2	2.6816283618075865
2209	3	4.568828500417748
2209	4	2.0611253719604754
2209	5	5.0991345020046595
2209	6	5.358611887663891
2209	7	6.3946769171576
2209	8	7.199983184686779
2209	9	6.36019570650744
2209	10-11	6.337023654829384
2209	12-13	5.734287556190907
2209	14-15	5.1183889396286375
2209	16-17	4.716560666977784
2209	18-19	3.9437764282569994
2209	20-21	4.256293172641971
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2209	24-25	4.317013894406003
2209	26-27	1.7199342243286893
2209	28-29	0.28664698491626694
2209	30-31	3.161468431563069
2209	32-33	4.48929795662805
2209	34-35	4.298827237356559
2209	36-37	4.071265113947234
2209	38-39	-1.216589992883744
2209	40-41	-0.011255818659837047
2209	42-43	-4.208530916577022
2209	44-45	0.8525721665938413
2209	46-47	2.743598198644399
2209	48-49	1.9995967342253422
2209	50-51	3.072481209167851
2209	52-53	3.9875026998239633
2209	54-55	3.366669040915106
2209	56-57	3.7684791876067614
2209	58-59	2.8680671406138067
2209	60-61	3.083566716568356
2209	62-63	2.8769392924577737
2209	64-65	2.529002875520664
2209	66-67	2.545785765061119
2209	68-69	-0.7022665716893499
2209	70-71	1.8664832790922254
2209	72-73	2.3333974803951207
2209	74-75	1.926887575663116
2209	76-77	2.272965181609827
2209	78-79	2.7121482415143383
2209	80-81	2.328848842574022
2209	82-83	2.360229437701232
2209	84-85	1.4831806411005743
2209	86-87	1.6189280968948907
2209	88-89	1.8435168109891116
2209	90-91	1.878936808988371
2209	92-93	1.7927193982427525
2209	94-95	1.973177000702485
2209	96-97	1.894935052597905
2209	98-99	1.6887673575669453
2209	100-101	1.5469446191490768
2208	1	4.570562433130345
2208	2	6.187296919237713
2208	3	4.5633167273383215
2208	4	3.0585621853784986
2208	5	3.9759422730786937
2208	6	5.020577135383164
2208	7	3.372416932958206
2208	8	3.7355210839665247
2208	9	4.1384602146027625
2208	10-11	4.40752271095592
2208	12-13	5.052383094646736
2208	14-15	5.599106217852302
2208	16-17	3.9622395386915272
2208	18-19	1.8645795170670745
2208	20-21	4.202175592523584
2208	22-23	3.4168470338412966
2208	24-25	3.9923079046555756
2208	26-27	5.120080635573366
2208	28-29	4.307733066085767
2208	30-31	4.356107605841206
2208	32-33	3.514736839690002
2208	34-35	3.725739882003122
2208	36-37	3.6901505808297586
2208	38-39	3.9236002727311217
2208	40-41	3.3669772646933254
2208	42-43	3.3269475381310762
2208	44-45	-1.3015573703108885
2208	46-47	2.6185354735921145
2208	48-49	2.770700009638208
2208	50-51	3.4009910293432135
2208	52-53	1.8743527008646694
2208	54-55	1.2118415534736897
2208	56-57	-0.7554018065100934
2208	58-59	2.1609923429402755
2208	60-61	3.2006491068577745
2208	62-63	2.6403108709164655
2208	64-65	2.9274625541150066
2208	66-67	3.0538946041691553
2208	68-69	2.1468467596410044
2208	70-71	-0.1057694541137515
2208	72-73	-0.014670452250506116
2208	74-75	-2.25741740587117
2208	76-77	0.2688116406364003
2208	78-79	1.8121923243869738
2208	80-81	2.0600469940118664
2208	82-83	2.2938692735052584
2208	84-85	1.5902710832731657
2208	86-87	2.0478568796963046
2208	88-89	2.359770970412324
2208	90-91	2.2683343860479894
2208	92-93	1.9922115345912914
2208	94-95	2.079502299045231
2208	96-97	2.21109182699405
2208	98-99	2.4786669952208875
2208	100-101	2.2873828664765057
1307	1	7.275794521174067
1307	2	7.08393226649633
1307	3	6.833463092540089
1307	4	8.37888174316343
1307	5	4.907177330851148
1307	6	5.802732309948311
1307	7	3.4995375438738314
1307	8	5.473551205500801
1307	9	5.959328298753398
1307	10-11	6.753623070318053
1307	12-13	5.82327101858175
1307	14-15	5.452270289125792
1307	16-17	4.1964583627787775
1307	18-19	5.028076559816196
1307	20-21	4.930415154800553
1307	22-23	4.598254098800982
1307	24-25	5.36291139595852
1307	26-27	6.196975584538947
1307	28-29	5.335354849919462
1307	30-31	5.18119395257575
1307	32-33	5.611247034094937
1307	34-35	5.385835899072827
1307	36-37	4.991745437459244
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1212	54-55	-4.268799545133266
1212	56-57	-3.964685364310565
1212	58-59	-2.1279344388278645
1212	60-61	-14.069073840368365
1212	62-63	-11.958575649049923
1212	64-65	-16.49461690903133
1212	66-67	-12.39662989972808
1212	68-69	-8.160306490871438
1212	70-71	-15.322055753095981
1212	72-73	-11.854109566001767
1212	74-75	-8.121856186145333
1212	76-77	-19.208115078882685
1212	78-79	-9.78494038393617
1212	80-81	-7.727625635966387
1212	82-83	-5.096707441789778
1212	84-85	-4.043273433696374
1212	86-87	-3.3939822131570594
1212	88-89	-2.8964731103691683
1212	90-91	-3.206283560355569
1212	92-93	-3.4009179478967155
1212	94-95	-3.131384449908758
1212	96-97	-12.95528489986153
1212	98-99	-10.134559873695931
1212	100-101	-6.143544855697055
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	306.0
3	632.0
4	282.0
5	1984.0
6	2325.0
7	2105.0
8	4225.0
9	8248.0
10	11030.0
11	13778.0
12	18256.0
13	23190.0
14	26484.0
15	28768.0
16	29958.0
17	33222.0
18	37563.0
19	41418.0
20	45242.0
21	50282.0
22	55957.0
23	61277.0
24	64830.0
25	66993.0
26	70483.0
27	73638.0
28	75589.0
29	76167.0
30	79150.0
31	83013.0
32	90348.0
33	105025.0
34	120004.0
35	131881.0
36	138605.0
37	128799.0
38	46771.0
39	153.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.581941699014912	28.899602521843704	17.086219961370603	23.43223581777078
2	24.48015249591398	30.34061727206358	24.409766953774675	20.76946327824776
3	20.000540435050215	27.526578896011287	29.04502124209989	23.427859426838605
4	22.893887320486066	26.997545108628945	27.572235178593345	22.53633239229164
5	22.014228935907752	28.312146610412643	27.002111017195336	22.67151343648427
6	21.810195292297692	28.654236062482553	26.760239458472352	22.7753291867474
7	22.808378914215048	28.424932537091834	27.495857329988365	21.270831218704746
8	21.548573092498042	27.796798388161527	28.33932286627034	22.315305653070094
9	21.841501895010083	27.381621294678222	28.500871469802696	22.276005340509
10-11	22.56735219757724	27.496142876639134	27.41078884244663	22.525716083336995
12-13	22.361000658471024	27.656813257788976	27.37439472032724	22.607791363412762
14-15	22.422319594454947	27.90817530159066	27.275073893987305	22.394431209967085
16-17	22.672048451638325	27.85315669554176	27.129930672531234	22.344864180288685
18-19	22.73157743689942	27.87916165646649	26.93240498271219	22.456855923921896
20-21	22.663283163376416	27.877383091566777	27.084461757180684	22.374871987876123
22-23	22.475444271577878	27.716746784686514	27.289425846156078	22.51838309757953
24-25	22.432559212161646	27.791069766293546	27.371502413038318	22.404868608506487
26-27	22.30577285356198	27.9231626071609	27.34054132090453	22.430523218372585
28-29	22.673057642419455	27.71031554043021	27.27606916991204	22.340557647238295
30-31	22.592382032098712	27.716118834517207	27.176321931959507	22.51517720142458
32-33	22.396761210453768	27.863153716328444	27.293607561120904	22.446477512096887
34-35	22.575673729253513	27.68238020443284	27.331929431568902	22.410016634744746
36-37	22.543732244710043	27.795149525068258	27.279947546728415	22.38117068349328
38-39	22.487270887637308	27.81943768859786	27.385609456923216	22.30768196684162
40-41	22.70424669337295	27.7156437590391	27.146228403326937	22.433881144261015
42-43	22.540524585436586	27.786215723960822	27.214131134117096	22.459128556485496
44-45	22.44172309433888	27.733401050196406	27.453739912399534	22.37113594306518
46-47	22.652500763611844	27.803704141781242	27.174921587423473	22.36887350718344
48-49	22.53115314972017	27.74215568419047	27.319149986087144	22.407541180002223
50-51	22.65450432816651	27.751756118373592	27.169608925523864	22.42413062793603
52-53	22.514634809483912	27.754617941305398	27.379689381370653	22.35105786784004
54-55	22.575102183968642	27.733462520045265	27.276281628502545	22.41515366748355
56-57	22.467857947247804	27.790549352941063	27.386955938019742	22.35463676179139
58-59	22.658149363730757	27.801566226583418	27.158096320681235	22.382188089004586
60-61	22.719333078482872	27.62313709207575	27.15631069106828	22.501219138373095
62-63	22.66147522264114	27.774661060386197	27.170725612639117	22.393138104333545
64-65	22.861744685490983	27.665089907076567	27.119899663077906	22.35326574435454
66-67	22.903642885750607	27.67230570985773	27.071434168596653	22.35261723579501
68-69	22.81350373135448	27.79109760431306	27.15391867902715	22.24147998530531
70-71	23.074312387638678	27.72067087651875	26.89900371934939	22.306013016493186
72-73	22.946255339602075	27.72190066139984	27.032907519107486	22.298936479890603
74-75	23.01777085501774	27.703769789519384	26.99432230756501	22.28413704789786
76-77	23.245391363147647	27.769258867488034	26.779721454282168	22.205628315082148
78-79	23.289127956247103	27.675283502677768	26.83774386035418	22.197844680720944
80-81	23.41300593813529	27.705753511488247	26.698273045684896	22.18296750469157
82-83	23.427400086541304	27.692836572104923	26.752468208581405	22.127295132772364
84-85	23.627171855439222	27.552106120398008	26.551108723808863	22.269613300353907
86-87	23.610446455968503	27.694602223870657	26.63146996079234	22.063481359368495
88-89	23.806422753176363	27.67029383237415	26.51883615150371	22.004447262945774
90-91	24.054854585305858	27.60206433135887	26.26422423200927	22.078856851326005
92-93	24.106720118510456	27.611516176617656	26.282284600172208	21.999479104699684
94-95	24.399650268780455	27.58606913034935	26.075522701138258	21.93875789973194
96-97	24.549571383068592	27.557452373407365	26.008462906829006	21.884513336695036
98-99	24.69470346451261	27.679172367935863	25.89569262907791	21.730431538473617
100-101	25.334915189890584	27.44720892709449	25.427548709882984	21.790327173131942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	131.5
2	134.0
3	149.0
4	157.5
5	164.5
6	205.5
7	264.0
8	335.5
9	439.5
10	592.5
11	798.5
12	1087.5
13	1426.0
14	1827.5
15	2410.0
16	3131.0
17	3948.0
18	4955.0
19	6301.0
20	7883.5
21	9845.5
22	12087.5
23	14736.5
24	17670.5
25	20767.0
26	24324.5
27	28110.5
28	32146.0
29	36129.5
30	40090.0
31	43985.5
32	47262.5
33	50289.0
34	53357.0
35	55852.5
36	58051.0
37	59647.0
38	59997.5
39	59791.0
40	58726.0
41	56902.5
42	54804.5
43	52070.5
44	49204.5
45	46735.5
46	44592.0
47	42767.0
48	40474.0
49	37777.5
50	35675.0
51	33827.5
52	31622.0
53	29716.0
54	28324.5
55	26904.5
56	25793.0
57	25036.5
58	24370.5
59	23642.5
60	22897.5
61	22123.0
62	21522.5
63	21046.0
64	20528.5
65	20178.5
66	19557.0
67	18700.5
68	17832.0
69	16954.0
70	15970.0
71	14605.5
72	13179.0
73	11767.0
74	10303.0
75	8847.0
76	7558.5
77	6441.0
78	5357.5
79	4380.0
80	3587.5
81	2990.0
82	2495.5
83	2014.0
84	1595.5
85	1278.5
86	1045.0
87	858.0
88	685.5
89	566.5
90	444.0
91	333.0
92	263.5
93	200.5
94	133.0
95	83.0
96	58.5
97	45.5
98	26.0
99	11.5
100	5.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	13.877144840774877
2	11.202442016449304
3	9.884084306061588
4	11.828097799706814
5	9.282454743852886
6	5.413313232116564
7	9.902266311179607
8	10.241122608944572
9	5.5238663168073705
10-11	6.42392968325973
12-13	5.451977049547587
14-15	7.44550403927313
16-17	2.7091458191399154
18-19	3.177224224707938
20-21	3.0917525667201127
22-23	3.1507629136879656
24-25	3.5599933116195457
26-27	4.418524865785957
28-29	1.7419821957043933
30-31	2.2940982618327785
32-33	3.07459871070103
34-35	2.0191224909779915
36-37	3.1492477465947974
38-39	1.3790996768906174
40-41	1.1469273764178312
42-43	0.5440261561130769
44-45	1.3367561679476143
46-47	1.4095112449749214
48-49	1.3056681859824317
50-51	1.5799404864011046
52-53	2.4768111793357184
54-55	1.871501925615036
56-57	1.8546998048140104
58-59	1.553262722939251
60-61	1.4068055894514067
62-63	0.6013319401011158
64-65	1.2408136230837872
66-67	0.5582849607219987
68-69	0.793595821602062
70-71	0.9862384948763001
72-73	0.08217075824913785
74-75	0.10262551400690807
76-77	0.44821889402542564
78-79	0.8985481993591926
80-81	0.2876111821496
82-83	0.5169154877674608
84-85	0.06347467858165208
86-87	0.0966189587447057
88-89	0.07857223640286345
90-91	0.08506580965929846
92-93	0.16669543680373336
94-95	0.4481918374701905
96-97	0.35993335429314477
98-99	0.06331233925024121
100-101	0.22489408711453204
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1847981.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.31383473939378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.44116038628661	98.7588296889722
2	0.46038156906269645	0.9144451813391087
3	0.0716296770105068	0.21341453715172884
4	0.02216883934550199	0.08806689788493423
5	0.002539064910661293	0.012608213646500464
6	0.0021204633840234154	0.012635481005510233
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.0822622094058327E-4	0.0	0.0
2	2.1645244188116654E-4	0.0	0.0
3	2.1645244188116654E-4	0.0	1.0822622094058327E-4
4	2.1645244188116654E-4	0.0	1.0822622094058327E-4
5	2.7056555235145815E-4	0.0	1.0822622094058327E-4
6	3.787917732920414E-4	0.0	1.0822622094058327E-4
7	3.787917732920414E-4	0.0	1.0822622094058327E-4
8	5.952442151732079E-4	0.0	1.0822622094058327E-4
9	5.952442151732079E-4	0.0	1.0822622094058327E-4
10-11	5.952442151732079E-4	0.0	1.0822622094058327E-4
12-13	7.30526991348937E-4	0.0	1.0822622094058327E-4
14-15	8.387532122895203E-4	0.0	1.0822622094058327E-4
16-17	9.740359884652494E-4	0.0	1.623393314108749E-4
18-19	0.0010552056541706868	0.0	1.623393314108749E-4
20-21	0.0011093187646409783	2.7056555235145817E-5	1.623393314108749E-4
22-23	0.0011904884303464158	5.4113110470291635E-5	1.623393314108749E-4
24-25	0.0012446015408167075	5.4113110470291635E-5	1.623393314108749E-4
26-27	0.0015422236484033116	5.4113110470291635E-5	1.8939588664602074E-4
28-29	0.002164524418811665	5.4113110470291635E-5	2.1645244188116654E-4
30-31	0.003409125959628373	5.4113110470291635E-5	2.4350899711631234E-4
32-33	0.005248971715618288	5.4113110470291635E-5	2.7056555235145815E-4
34-35	0.009226285335184723	5.4113110470291635E-5	3.246786628217498E-4
36-37	0.014664652937449032	5.4113110470291635E-5	4.329048837623331E-4
38-39	0.02215931873758442	5.4113110470291635E-5	4.329048837623331E-4
40-41	0.03219730072982352	5.4113110470291635E-5	4.329048837623331E-4
42-43	0.04672667089109682	5.4113110470291635E-5	4.329048837623331E-4
44-45	0.06669440865463444	5.4113110470291635E-5	4.329048837623331E-4
46-47	0.09488733920965638	5.4113110470291635E-5	4.329048837623331E-4
48-49	0.13092667078287062	5.4113110470291635E-5	4.329048837623331E-4
50-51	0.17324312317063867	5.4113110470291635E-5	4.329048837623331E-4
52-53	0.22608457554487843	5.4113110470291635E-5	4.329048837623331E-4
54-55	0.2853654880650829	5.4113110470291635E-5	4.329048837623331E-4
56-57	0.3579852823162143	5.4113110470291635E-5	4.329048837623331E-4
58-59	0.45103277576988077	5.4113110470291635E-5	4.329048837623331E-4
60-61	0.5606388810274565	5.4113110470291635E-5	4.329048837623331E-4
62-63	0.6820687009227908	5.4113110470291635E-5	4.329048837623331E-4
64-65	0.8166750632176413	5.4113110470291635E-5	4.329048837623331E-4
66-67	0.972683160703492	5.4113110470291635E-5	5.140745494677705E-4
68-69	1.1533397800085607	5.4113110470291635E-5	5.952442151732079E-4
70-71	1.3686829031250864	5.4113110470291635E-5	5.952442151732079E-4
72-73	1.5834307820264386	5.4113110470291635E-5	5.952442151732079E-4
74-75	1.8162253832696331	5.4113110470291635E-5	5.952442151732079E-4
76-77	2.064361051331155	5.4113110470291635E-5	5.952442151732079E-4
78-79	2.347426732201251	5.4113110470291635E-5	5.952442151732079E-4
80-81	2.679221269049844	5.4113110470291635E-5	5.952442151732079E-4
82-83	3.027493248036641	5.4113110470291635E-5	5.952442151732079E-4
84-85	3.3914039159493523	5.4113110470291635E-5	5.952442151732079E-4
86-87	3.786023774053954	5.4113110470291635E-5	5.952442151732079E-4
88-89	4.219199223368639	5.4113110470291635E-5	5.952442151732079E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCTAG	415	0.008377804	6.051802	72-73
CGTGTAG	3605	0.0	6.0340323	94-95
GGAGTTC	1150	0.004094273	5.8993735	1
TCGTGTA	3755	0.0	5.792992	94-95
GTCGTGT	4230	0.0	5.3566756	92-93
GTGTAGG	3530	0.0	5.327765	94-95
AGCGTCG	7195	0.0	5.1333213	94-95
GAGCGTC	7505	0.0	5.0420537	94-95
>>END_MODULE