FastQCFastQC Report
Thu 11 Sep 2014
NYCLib18_GTCCGC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib18_GTCCGC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847981
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAG4150.0083778046.05180272-73
CGTGTAG36050.06.034032394-95
GGAGTTC11500.0040942735.89937351
TCGTGTA37550.05.79299294-95
GTCGTGT42300.05.356675692-93
GTGTAGG35300.05.32776594-95
AGCGTCG71950.05.133321394-95
GAGCGTC75050.05.042053794-95
Produced by FastQC (version 0.11.2)