##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib18_GTCCGC_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1847981 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20296150230982 33.0 33.0 33.0 27.0 33.0 2 31.63792160200781 33.0 33.0 33.0 27.0 33.0 3 31.785117379453578 33.0 33.0 33.0 27.0 33.0 4 35.602134978660494 37.0 37.0 37.0 33.0 37.0 5 35.10525270552024 37.0 37.0 37.0 33.0 37.0 6 34.898692681364146 37.0 33.0 37.0 27.0 37.0 7 34.69444112250072 37.0 33.0 37.0 27.0 37.0 8 34.74083553889353 37.0 33.0 37.0 27.0 37.0 9 34.64536053130416 37.0 33.0 37.0 27.0 37.0 10-11 34.4921122024523 37.0 33.0 37.0 27.0 37.0 12-13 34.49655732391188 37.0 33.0 37.0 27.0 37.0 14-15 35.39786123342177 37.0 33.0 40.0 27.0 40.0 16-17 35.30632187235691 37.0 33.0 40.0 27.0 40.0 18-19 35.513513937643296 37.0 33.0 40.0 27.0 40.0 20-21 35.19884511799634 37.0 33.0 40.0 27.0 40.0 22-23 35.221214125036994 37.0 33.0 40.0 27.0 40.0 24-25 35.064027985136214 37.0 33.0 40.0 27.0 40.0 26-27 34.6733080589032 37.0 33.0 40.0 27.0 40.0 28-29 34.292716212991365 37.0 33.0 40.0 27.0 40.0 30-31 34.186221611585836 37.0 33.0 40.0 24.5 40.0 32-33 34.183715092308844 37.0 33.0 38.5 24.5 40.0 34-35 34.03536183543012 37.0 33.0 37.0 24.5 40.0 36-37 33.72349932169216 37.0 33.0 37.0 22.0 40.0 38-39 33.63263637450818 37.0 33.0 37.0 22.0 40.0 40-41 33.348427554179395 37.0 33.0 37.0 22.0 40.0 42-43 32.96386732331122 37.0 33.0 37.0 22.0 40.0 44-45 32.85341380674369 37.0 33.0 37.0 22.0 40.0 46-47 32.71851469252119 37.0 33.0 37.0 22.0 40.0 48-49 32.754122742603954 37.0 33.0 37.0 22.0 40.0 50-51 33.05482145108635 37.0 33.0 37.0 22.0 40.0 52-53 32.91043279124623 37.0 33.0 37.0 22.0 40.0 54-55 32.557863149025884 37.0 33.0 37.0 22.0 40.0 56-57 32.607141523641204 37.0 33.0 37.0 22.0 40.0 58-59 32.321760072208534 37.0 30.0 37.0 22.0 40.0 60-61 32.23270774970089 37.0 27.0 37.0 22.0 40.0 62-63 32.2224968763207 35.0 27.0 37.0 22.0 40.0 64-65 31.878141874835293 33.0 27.0 37.0 22.0 40.0 66-67 31.544997757011572 33.0 27.0 37.0 18.5 40.0 68-69 31.368411525876077 33.0 27.0 37.0 15.0 40.0 70-71 31.21920274072082 33.0 27.0 37.0 15.0 40.0 72-73 30.995500494864395 33.0 27.0 37.0 15.0 37.0 74-75 30.64121276138662 33.0 27.0 37.0 15.0 37.0 76-77 29.553613105329546 33.0 27.0 37.0 15.0 37.0 78-79 30.47330329694948 33.0 27.0 37.0 15.0 37.0 80-81 30.1818511662187 33.0 27.0 37.0 15.0 37.0 82-83 30.16278874079333 33.0 27.0 37.0 15.0 37.0 84-85 30.024745925418063 33.0 27.0 37.0 15.0 37.0 86-87 29.80942498867683 33.0 27.0 37.0 15.0 37.0 88-89 29.66464482048246 33.0 27.0 37.0 15.0 37.0 90-91 29.567620283974783 33.0 27.0 37.0 15.0 37.0 92-93 29.3564817495418 33.0 27.0 33.0 15.0 37.0 94-95 29.24405066935212 33.0 27.0 33.0 15.0 37.0 96-97 29.11269298764435 33.0 27.0 33.0 15.0 37.0 98-99 28.839818158303576 33.0 27.0 33.0 15.0 37.0 100-101 28.022956945985918 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.156790065735315 2306 2 0.5056062163078074 2306 3 0.4608724763377019 2306 4 0.3660825131951455 2306 5 0.7490423459879949 2306 6 0.762233484186055 2306 7 0.8394245146247599 2306 8 0.8954181037158051 2306 9 1.0503865383425222 2306 10-11 1.1771622689359944 2306 12-13 1.3908541497167377 2306 14-15 1.474545515903941 2306 16-17 1.5034483616036027 2306 18-19 1.2736401409888387 2306 20-21 1.5616458319375042 2306 22-23 1.2668336602665065 2306 24-25 1.2445374919968017 2306 26-27 1.2527018636632334 2306 28-29 1.545243159857172 2306 30-31 1.4492579263009802 2306 32-33 1.0346947381027363 2306 34-35 0.9997284356213711 2306 36-37 1.1740574577210197 2306 38-39 0.9471687475441612 2306 40-41 1.1624415689814356 2306 42-43 1.1285562195502195 2306 44-45 0.8175981837686948 2306 46-47 0.7412410035830064 2306 48-49 0.8650966728652563 2306 50-51 0.9576759806765409 2306 52-53 1.0025365115526625 2306 54-55 1.4243840938687455 2306 56-57 0.7866680851076779 2306 58-59 1.0112062896321774 2306 60-61 0.7811799977659213 2306 62-63 0.5022628710460744 2306 64-65 0.5982953135655258 2306 66-67 0.7753563811827995 2306 68-69 0.5320376595140743 2306 70-71 0.5126927246464312 2306 72-73 0.3915224425263091 2306 74-75 0.6675209605549206 2306 76-77 0.6525896460419389 2306 78-79 0.7178851049265909 2306 80-81 1.2657106254644397 2306 82-83 1.0246381415533108 2306 84-85 0.7553396561002295 2306 86-87 0.5771964542901351 2306 88-89 0.5545913923799333 2306 90-91 0.17481942597469313 2306 92-93 -0.044281628839705434 2306 94-95 -0.08850781913305994 2306 96-97 0.12109322562600866 2306 98-99 0.22006473221471268 2306 100-101 0.23730748221875686 2307 1 0.5033011617137042 2307 2 0.4658874117797147 2307 3 0.3548077104338141 2307 4 0.3690194246394114 2307 5 0.6048182821762467 2307 6 0.632003381523603 2307 7 0.7759464857994018 2307 8 0.7343898873125596 2307 9 0.8869238202239345 2307 10-11 1.1601615792712252 2307 12-13 1.4016278333808927 2307 14-15 1.4133055758950874 2307 16-17 1.2847698395077458 2307 18-19 1.2036755195246442 2307 20-21 1.511044318598934 2307 22-23 1.1656243627808323 2307 24-25 1.2114817319673534 2307 26-27 1.3349760201836034 2307 28-29 1.5570914800503672 2307 30-31 1.6581109296084904 2307 32-33 1.1646751570374505 2307 34-35 1.1883905840773608 2307 36-37 1.2015702576208795 2307 38-39 0.9370387841060719 2307 40-41 1.1963158557856133 2307 42-43 1.3199502364290012 2307 44-45 1.0891962142629552 2307 46-47 1.0219220518833794 2307 48-49 1.05409375079266 2307 50-51 1.1215724090226828 2307 52-53 1.145219477602879 2307 54-55 1.5123762033289907 2307 56-57 1.3041500748488772 2307 58-59 1.2354472982861466 2307 60-61 0.8686883857484311 2307 62-63 0.8036226244753983 2307 64-65 0.9576148774450708 2307 66-67 1.0487482043961585 2307 68-69 0.8278142513432982 2307 70-71 0.7994940820715897 2307 72-73 0.6046459285296137 2307 74-75 0.8702100384703968 2307 76-77 0.9367432321745355 2307 78-79 0.9940186994732478 2307 80-81 1.4570651548756537 2307 82-83 1.15369954555878 2307 84-85 0.9812093365298971 2307 86-87 1.0742822046786777 2307 88-89 0.7963878709918824 2307 90-91 0.6473665712791359 2307 92-93 0.6454173139889008 2307 94-95 0.5497158880497359 2307 96-97 0.6212336979457938 2307 98-99 0.5632574529111629 2307 100-101 0.6647303239554709 1101 1 1.0483547333240466 1101 2 0.5052641560428057 1101 3 0.3596204713916329 1101 4 0.34706746600989646 1101 5 0.5501762701954647 1101 6 0.5178965438067991 1101 7 0.6639635070189627 1101 8 0.7349084599190192 1101 9 1.031190061798469 1101 10-11 1.0055514201164826 1101 12-13 1.2998441446706366 1101 14-15 1.3385870526421684 1101 16-17 1.2896184491205105 1101 18-19 0.9888871337554335 1101 20-21 1.242310661902728 1101 22-23 1.0174610183529609 1101 24-25 1.1333980821974592 1101 26-27 1.0931247766746992 1101 28-29 1.3066309007044907 1101 30-31 1.26505723559427 1101 32-33 0.8453270246796691 1101 34-35 1.0074286603436988 1101 36-37 1.1838298520834485 1101 38-39 0.874032907898247 1101 40-41 1.1800563727257511 1101 42-43 1.08572675844308 1101 44-45 0.936136240727734 1101 46-47 0.862458130266873 1101 48-49 0.7511950614308631 1101 50-51 0.777685398166355 1101 52-53 1.023271299836793 1101 54-55 1.48650226275317 1101 56-57 1.1365469279312919 1101 58-59 1.1904420306835135 1101 60-61 0.8373176830268996 1101 62-63 0.5908040811990851 1101 64-65 0.6743099437913251 1101 66-67 0.616570192494283 1101 68-69 0.6020443224469254 1101 70-71 0.6291430440759989 1101 72-73 0.6877294856746765 1101 74-75 0.9346684490674875 1101 76-77 0.8875006472348765 1101 78-79 0.7718997695688721 1101 80-81 1.3412860121794772 1101 82-83 1.088706629619704 1101 84-85 0.9561040239931664 1101 86-87 0.8404822086871526 1101 88-89 0.5947626750650734 1101 90-91 0.42183212442040485 1101 92-93 0.5526243351661009 1101 94-95 0.6974861933886025 1101 96-97 0.7535110618342351 1101 98-99 0.6447896231590171 1101 100-101 0.6679350482897455 2201 1 1.5044924788340879 2201 2 0.8069585486085131 2201 3 0.3460033596747252 2201 4 0.21491437991422657 2201 5 0.39999185510018265 2201 6 0.4010811038934534 2201 7 0.4776768792045303 2201 8 0.5767851121992251 2201 9 0.6361873629396726 2201 10-11 0.8342753292576859 2201 12-13 1.1049659633033642 2201 14-15 1.2211736271567801 2201 16-17 1.046136079694385 2201 18-19 0.9230080448022377 2201 20-21 1.1238673120256308 2201 22-23 0.9703383111129114 2201 24-25 0.9065123045347292 2201 26-27 1.138940708434049 2201 28-29 1.4145732073018777 2201 30-31 1.4461905990931356 2201 32-33 1.0327389491305325 2201 34-35 0.896509621442874 2201 36-37 1.1409467206889943 2201 38-39 0.91930740647188 2201 40-41 1.2158285367721433 2201 42-43 1.0182684840397798 2201 44-45 0.8858957716739368 2201 46-47 0.9222801071887687 2201 48-49 1.0515636360174199 2201 50-51 0.9508436930243676 2201 52-53 1.0527706540213302 2201 54-55 1.4955005781310646 2201 56-57 1.0259550302076548 2201 58-59 1.0821375332096181 2201 60-61 0.8454186685912255 2201 62-63 0.816933111251533 2201 64-65 0.8333243270760455 2201 66-67 0.8653752277909597 2201 68-69 0.826566018658113 2201 70-71 0.8452509273700208 2201 72-73 0.632648899163172 2201 74-75 0.8000694243373516 2201 76-77 0.9680403193089973 2201 78-79 0.8955709881807827 2201 80-81 1.4167289723243606 2201 82-83 1.4523846421860895 2201 84-85 1.3726526069341425 2201 86-87 1.205305071596431 2201 88-89 1.0926479992223932 2201 90-91 1.0192329384041408 2201 92-93 1.1341754003414763 2201 94-95 0.9297128487073003 2201 96-97 0.7613424810237177 2201 98-99 0.9447542156140862 2201 100-101 0.9769152413436224 2304 1 -1.7746434072432393 2304 2 -0.17683215525662632 2304 3 -0.04517994312108442 2304 4 -0.02587752789990816 2304 5 0.3588571070098112 2304 6 0.5806433740354606 2304 7 0.5295200310763022 2304 8 0.7734238397957682 2304 9 1.1038302297548768 2304 10-11 0.9534999095164736 2304 12-13 1.1888793581628505 2304 14-15 1.147436761450038 2304 16-17 1.1882812070274653 2304 18-19 1.025128744278824 2304 20-21 1.292825557412975 2304 22-23 1.0335251468122095 2304 24-25 1.1462119850205141 2304 26-27 1.2750934735695552 2304 28-29 1.6074969259083502 2304 30-31 1.6585999307160577 2304 32-33 1.1674551943609615 2304 34-35 1.135508303257069 2304 36-37 1.2970029800513672 2304 38-39 0.9197267480962026 2304 40-41 1.0056641858486444 2304 42-43 1.0269607052993024 2304 44-45 1.1001405190316618 2304 46-47 1.078365655963232 2304 48-49 1.0351141017961538 2304 50-51 0.979091221496958 2304 52-53 1.2291409461844296 2304 54-55 1.5526648291784895 2304 56-57 1.1260027127214585 2304 58-59 0.8417720256869856 2304 60-61 0.4728455012463719 2304 62-63 0.3460548597109785 2304 64-65 0.4428864033075435 2304 66-67 0.5261530458563293 2304 68-69 0.5028359479327023 2304 70-71 0.45531773887188365 2304 72-73 0.27556857323448725 2304 74-75 0.7319312581482542 2304 76-77 0.7318527314154046 2304 78-79 0.551837952815152 2304 80-81 1.2418304122681256 2304 82-83 1.0057051244180073 2304 84-85 0.7032733972403697 2304 86-87 0.5811882264766517 2304 88-89 0.4659238284879983 2304 90-91 0.32974058395487305 2304 92-93 0.3112009315406645 2304 94-95 0.2199249194663011 2304 96-97 0.29741429866215796 2304 98-99 0.4975535734863179 2304 100-101 0.6722607724324625 1102 1 1.4823137243904085 1102 2 0.09436527675069684 1102 3 0.24011355123292688 1102 4 0.18253602233490795 1102 5 0.25009482167705244 1102 6 0.07553372624159493 1102 7 -4.862886827374648 1102 8 -1.2757144433252847 1102 9 0.13640444734650004 1102 10-11 0.6090177267227048 1102 12-13 0.8851261305120346 1102 14-15 0.8226749379658287 1102 16-17 0.5526156162145242 1102 18-19 0.8568061043410538 1102 20-21 1.0983135857463822 1102 22-23 0.5754799907178025 1102 24-25 0.9275596863378865 1102 26-27 1.0904668268446116 1102 28-29 0.8353901847482916 1102 30-31 1.2024758117871883 1102 32-33 1.0419107832124794 1102 34-35 1.1158162267924467 1102 36-37 1.3152493593989831 1102 38-39 0.8961512711784039 1102 40-41 0.9829682561041295 1102 42-43 1.0938189751673093 1102 44-45 1.2059406357846782 1102 46-47 0.9769063956308912 1102 48-49 0.8698482054029739 1102 50-51 0.8719430305053706 1102 52-53 0.8654840702527906 1102 54-55 1.1973968422751895 1102 56-57 0.9071868127962972 1102 58-59 0.9432119228037692 1102 60-61 0.8791764390391741 1102 62-63 0.5572396805277329 1102 64-65 0.43378129941492816 1102 66-67 0.361083784710015 1102 68-69 0.6062351156012937 1102 70-71 0.4758836700829825 1102 72-73 0.4121063155480975 1102 74-75 0.45733490947698385 1102 76-77 0.4453738227979471 1102 78-79 0.6952249844091121 1102 80-81 1.315814569572023 1102 82-83 1.017397413878001 1102 84-85 0.645431796949488 1102 86-87 0.5410645062865918 1102 88-89 0.4631449829444243 1102 90-91 0.148351673053277 1102 92-93 0.22177160673957985 1102 94-95 0.08753489446265306 1102 96-97 0.03782520402515743 1102 98-99 0.1567802980944215 1102 100-101 0.20377857421983236 2202 1 1.575748251275165 2202 2 0.9156805314438259 2202 3 0.44551592901025217 2202 4 -0.3981645243289833 2202 5 0.08728369333604746 2202 6 0.48202017711507494 2202 7 0.6199261248537979 2202 8 -4.221450121075286 2202 9 -0.5097665096418282 2202 10-11 0.527360701799978 2202 12-13 0.09573919433766065 2202 14-15 0.10745737949419265 2202 16-17 0.3367928323826703 2202 18-19 0.7234358810809951 2202 20-21 1.101910112090927 2202 22-23 -2.857544167289589 2202 24-25 -2.964528583631452 2202 26-27 -1.4371208231189314 2202 28-29 0.739089555932182 2202 30-31 1.3195341247917725 2202 32-33 1.0671550566116679 2202 34-35 -1.9039710681313124 2202 36-37 -0.08356550984335342 2202 38-39 0.2014825385985617 2202 40-41 1.1806056842837265 2202 42-43 1.3939465603442045 2202 44-45 1.386234662282824 2202 46-47 0.6845662688320715 2202 48-49 0.9294003038588983 2202 50-51 -3.9649361763663578 2202 52-53 -0.2506706069944755 2202 54-55 1.5243783684593737 2202 56-57 1.410274379873428 2202 58-59 1.429348011469429 2202 60-61 1.4388383764790582 2202 62-63 1.2535147961726274 2202 64-65 1.166929081826698 2202 66-67 1.3329839392381082 2202 68-69 1.1827359392487722 2202 70-71 1.265160960481797 2202 72-73 1.0980869970250922 2202 74-75 0.8060247535539311 2202 76-77 1.1375679419974958 2202 78-79 1.2650504090135826 2202 80-81 1.8849028074687695 2202 82-83 1.7318167800848414 2202 84-85 1.4517271260587705 2202 86-87 1.503942890476889 2202 88-89 1.384431633131701 2202 90-91 1.2390281981149158 2202 92-93 1.283803341994588 2202 94-95 1.2913489541617373 2202 96-97 1.1690298507648258 2202 98-99 1.2995682240368644 2202 100-101 1.1871086004691271 2305 1 1.4045655768461671 2305 2 0.6958222322414471 2305 3 0.12568435586177884 2305 4 -0.14565400802491268 2305 5 0.366934220180255 2305 6 0.4702524140745723 2305 7 0.5829333633189506 2305 8 -0.02639613455853862 2305 9 0.6428250147559851 2305 10-11 0.41213501580789824 2305 12-13 0.8613956094373592 2305 14-15 0.6613826607149775 2305 16-17 0.7728178595433093 2305 18-19 0.8043378220340287 2305 20-21 0.8631628406326186 2305 22-23 0.5144712878389583 2305 24-25 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0.8140267429300678 2305 92-93 0.5516026079085066 2305 94-95 0.5878384651108384 2305 96-97 0.9015810628973533 2305 98-99 1.0382190439367953 2305 100-101 1.1111855786821785 1103 1 1.3217740078235671 1103 2 0.6688439250348459 1103 3 0.2516324647718733 1103 4 -0.24992402356249954 1103 5 0.12276132736907641 1103 6 0.23046799165737042 1103 7 0.46539366897691536 1103 8 0.2560764780974978 1103 9 0.33412106130379016 1103 10-11 0.5676474398933706 1103 12-13 0.034068262091139445 1103 14-15 0.21027733845110674 1103 16-17 0.3092874262173311 1103 18-19 0.5175023785633144 1103 20-21 0.5679659884892345 1103 22-23 0.35776713610734845 1103 24-25 0.5995834713228874 1103 26-27 0.7116954559884761 1103 28-29 0.3466913408210601 1103 30-31 0.07077372088362921 1103 32-33 -0.07299924766716259 1103 34-35 0.5062237576573665 1103 36-37 0.8932514383276029 1103 38-39 0.0030469242741233415 1103 40-41 -3.1489941425750985 1103 42-43 -0.8187118701262435 1103 44-45 0.10802952716016279 1103 46-47 -3.131593114574308 1103 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-2.6903803488453306 1208 38-39 -0.2683718695224684 1208 40-41 -0.7968937716799758 1208 42-43 0.11097402573101078 1208 44-45 0.10053806393144527 1208 46-47 -0.03051315836957258 1208 48-49 -1.3007347115766272 1208 50-51 -0.406355480516126 1208 52-53 -1.6851344306264053 1208 54-55 -0.905843965589618 1208 56-57 -6.507964466018347 1208 58-59 -2.0421713470944596 1208 60-61 -1.6521243883543484 1208 62-63 0.2640026828922011 1208 64-65 -0.23283068181497057 1208 66-67 0.7129184324407483 1208 68-69 0.4287589132145584 1208 70-71 -4.0304742420350586 1208 72-73 -0.8410117850680656 1208 74-75 -5.566266265681559 1208 76-77 -1.3876853103113262 1208 78-79 -3.641081347030674 1208 80-81 0.9087140750324316 1208 82-83 0.10854959079357585 1208 84-85 -2.959277873199408 1208 86-87 -1.0324146831274987 1208 88-89 -4.969355121845471 1208 90-91 -0.03249292318954389 1208 92-93 -2.0632769195709315 1208 94-95 -4.638857746057159 1208 96-97 -3.4834785815355467 1208 98-99 -6.299871644160508 1208 100-101 -3.7035831220159103 1209 1 1.9288552538215313 1209 2 1.503311011051892 1209 3 0.527183332977831 1209 4 0.34838601950252723 1209 5 0.5599504242591067 1209 6 1.001447438466414 1209 7 0.8335481506272018 1209 8 0.26022980465091194 1209 9 -0.1699531662251843 1209 10-11 -3.2675390705841068 1209 12-13 -0.47500050383852255 1209 14-15 -1.2029648426731114 1209 16-17 -0.24138742657059709 1209 18-19 -16.781814607638296 1209 20-21 -14.148964969362286 1209 22-23 -3.4028465718791416 1209 24-25 -1.7984089201042863 1209 26-27 -1.169398621629007 1209 28-29 -3.5749491478351345 1209 30-31 -0.7795797136462355 1209 32-33 -0.41772409606070227 1209 34-35 -3.4532260646837116 1209 36-37 -4.342041793055877 1209 38-39 -1.7418486775690063 1209 40-41 -10.931892431273983 1209 42-43 -5.089392498398496 1209 44-45 -2.9417777474390014 1209 46-47 -2.725756108786271 1209 48-49 -3.0723580970466244 1209 50-51 -3.0595265295046623 1209 52-53 -1.4138472453471422 1209 54-55 -3.1337693833494846 1209 56-57 -0.4556241757581887 1209 58-59 -1.5134024257623722 1209 60-61 -1.105821052281609 1209 62-63 -0.9153341164526871 1209 64-65 -0.5301510417762394 1209 66-67 0.2566391064818454 1209 68-69 -0.6278728735978945 1209 70-71 -0.5414157637108126 1209 72-73 -0.06583888734470733 1209 74-75 1.1271411342384603 1209 76-77 -16.378314920245863 1209 78-79 -16.01003285656221 1209 80-81 -21.872441574668944 1209 82-83 -9.595990503459674 1209 84-85 -3.0375136352311998 1209 86-87 -1.9930609492544882 1209 88-89 -2.1710393456332078 1209 90-91 -5.653721291746987 1209 92-93 -3.020126101190815 1209 94-95 -0.44602239918499365 1209 96-97 0.17377740963974375 1209 98-99 1.361954179236978 1209 100-101 0.843839416434701 1212 1 -28.584334194619714 1212 2 -16.297648221562014 1212 3 -6.180046709633341 1212 4 -1.7300693542402144 1212 5 -0.5696306620756815 1212 6 -2.8575276607479516 1212 7 -0.5468961295826631 1212 8 -1.3061677995771035 1212 9 -0.17065372937017287 1212 10-11 0.6089924489835425 1212 12-13 0.5056993664418101 1212 14-15 -0.14129796429779162 1212 16-17 -0.18080218689716787 1212 18-19 -1.4301973460703437 1212 20-21 -0.8972106745637731 1212 22-23 -0.7748994913413298 1212 24-25 -0.12479051146041797 1212 26-27 -0.7390411419626588 1212 28-29 0.3196468928283167 1212 30-31 -0.978857209787364 1212 32-33 0.4373035992790122 1212 34-35 -1.2799810331006256 1212 36-37 -1.7997943710897566 1212 38-39 -0.40048773742866217 1212 40-41 -0.32460811426530967 1212 42-43 0.1521805353093697 1212 44-45 1.2047130855767634 1212 46-47 -1.8578546033453591 1212 48-49 -1.1372564191593995 1212 50-51 -0.19327827610096904 1212 52-53 -1.2155089657300664 1212 54-55 -0.46820552624896905 1212 56-57 -1.5190116680115793 1212 58-59 -0.29116801425465866 1212 60-61 0.2690302705124843 1212 62-63 -4.795741828806079 1212 64-65 -4.412223553910994 1212 66-67 -1.5911539271490334 1212 68-69 0.5186872458827629 1212 70-71 -0.3645774590736224 1212 72-73 -1.0698074620836309 1212 74-75 -0.33100984096853736 1212 76-77 0.8647804910655381 1212 78-79 -1.3643830842452296 1212 80-81 0.6591241839601061 1212 82-83 0.7954857216597517 1212 84-85 -2.8069860188010693 1212 86-87 -1.6787159565488103 1212 88-89 -0.7366613263792665 1212 90-91 0.031367876084075874 1212 92-93 -2.4463206190917433 1212 94-95 -2.156200965802004 1212 96-97 -0.04433060795901511 1212 98-99 -0.6962849437503458 1212 100-101 0.37055319789748964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 11.0 12 57.0 13 220.0 14 672.0 15 1428.0 16 2699.0 17 4482.0 18 6837.0 19 9902.0 20 13205.0 21 17937.0 22 23337.0 23 29162.0 24 36603.0 25 44964.0 26 54854.0 27 65865.0 28 78365.0 29 92202.0 30 107383.0 31 124275.0 32 143237.0 33 162340.0 34 180198.0 35 195435.0 36 202789.0 37 183997.0 38 65376.0 39 146.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.96257787081985 28.862210128205895 19.058973404727748 23.116238596246507 2 23.67289908269789 29.321523291875888 26.42577948492331 20.579798140502913 3 19.876621707544746 26.40062771411951 31.04592865163219 22.676821926703553 4 22.65948621766133 26.455358577820874 29.13314584944326 21.752009355074538 5 21.463099458273653 28.32610291988933 27.837786211005415 22.373011410831605 6 21.095942003732723 28.852569371654795 27.684321429711666 22.367167194900812 7 21.966026706984543 28.455325027692385 28.385356775854298 21.193291489468777 8 21.082089047452328 27.674959861600307 29.025460759607373 22.217490331339988 9 21.52062169470357 27.190214618007435 29.002408574547033 22.28675511274196 10-11 21.908477413999385 27.45499006753857 28.11500767594472 22.52152484251732 12-13 22.03016480613586 27.543879765822783 27.85878886994067 22.56716655810069 14-15 22.004209460002418 27.78630510440992 27.72810643835257 22.48137899723509 16-17 22.1263875664951 27.670349347353007 27.72402974175239 22.479233344399503 18-19 22.409945782192636 27.72202322134387 27.446139744123155 22.421891252340338 20-21 22.15868137101051 27.614178724877974 27.66187945756989 22.56526044654163 22-23 22.055096886818642 27.54397907770697 27.739652085167542 22.661271950306848 24-25 22.130638789035167 27.492517509649723 27.86305703359504 22.51378666772007 26-27 22.24002844185086 27.64297901331237 27.69579340913137 22.4211991357054 28-29 22.406507426212716 27.517923615015523 27.630560054459437 22.445008904312328 30-31 22.45217889144964 27.452527921012177 27.485888653617106 22.609404533921072 32-33 22.293194572887927 27.480829077788137 27.601636596913064 22.624339752410876 34-35 22.463704983979813 27.4025815200481 27.629829527468086 22.503883968504006 36-37 22.421063852929223 27.54381673837556 27.54819990032365 22.48691950837157 38-39 22.347659418576274 27.458101571390614 27.76668158384745 22.42755742618566 40-41 22.465121552030396 27.400797693231997 27.536325995215787 22.59775475952182 42-43 22.263729984236853 27.46575857652216 27.758645786942616 22.511865652298376 44-45 22.259319765733522 27.349036597237742 27.867602534874546 22.52404110215419 46-47 22.324387856422405 27.45213985373559 27.757869972820682 22.46560231702132 48-49 22.44583882018172 27.425764192730494 27.70433871154956 22.42405827553823 50-51 22.423294624400697 27.49496747800301 27.565801577939155 22.51593631965714 52-53 22.346249605650282 27.459998192620155 27.718577970853563 22.475174230876 54-55 22.237383211454674 27.324765435325578 27.880193371608293 22.557657981611463 56-57 22.36328841059325 27.54248558064732 27.658181320332986 22.43604468842645 58-59 22.35996448770151 27.448688357913703 27.79492785142469 22.396419302960098 60-61 22.500696041747894 27.39309164022069 27.54404273493404 22.562169583097376 62-63 22.460914987832524 27.412215314300802 27.610812794006794 22.51605690385988 64-65 22.51211012932533 27.394069006159032 27.604059987992013 22.489760876523626 66-67 22.46823540998901 27.468340935693718 27.593050672361088 22.47037298195619 68-69 22.506403273367415 27.54465515403302 27.491269739480263 22.457671833119306 70-71 22.65523322797123 27.50367576828563 27.33865176553225 22.50243923821089 72-73 22.466437734923677 27.446609885747957 27.533007393861936 22.553944985466433 74-75 22.48519076332398 27.43265600452154 27.57251951376114 22.50963371839334 76-77 22.502545263966297 27.609413831873557 27.431509568485286 22.45653133567486 78-79 22.71032419549079 27.563854371367135 27.242123741426084 22.483697691715985 80-81 22.539956941526505 27.532990006836915 27.309490091892023 22.617562959744554 82-83 22.650551358278168 27.590480174425874 27.093859973342582 22.665108493953376 84-85 22.567037596035675 27.425196752388274 27.243853906176856 22.76391174539919 86-87 22.560919512860362 27.585115225927677 27.171661305475343 22.682303955736618 88-89 22.678921642905696 27.57296595823899 27.01961957909958 22.72849281975574 90-91 22.769477744707544 27.454815291112766 26.900929484629376 22.87477747955031 92-93 22.639169555715988 27.50819631320145 26.967277525162604 22.885356605919963 94-95 22.74519174306316 27.464473533953164 26.804763958787838 22.985570764195838 96-97 22.739170235570665 27.50716464185991 26.705882894128365 23.047782228441065 98-99 22.687070476433757 27.521574100958485 26.706370607213763 23.084984815393994 100-101 22.848714070819664 27.375626403903773 26.516121368183292 23.259538157093264 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 2.0 4 5.5 5 8.5 6 16.0 7 28.5 8 42.0 9 62.0 10 101.5 11 166.0 12 266.5 13 374.5 14 532.0 15 814.5 16 1222.0 17 1758.5 18 2552.0 19 3611.0 20 4947.0 21 6719.0 22 8869.5 23 11393.5 24 14300.5 25 17682.5 26 21456.0 27 25316.0 28 29377.5 29 33656.5 30 37888.5 31 42038.5 32 45987.0 33 49642.5 34 53050.0 35 56148.0 36 58912.5 37 60822.0 38 61804.5 39 61835.0 40 61021.5 41 59335.5 42 56801.5 43 53774.5 44 50863.5 45 48618.5 46 46411.5 47 44377.5 48 42402.5 49 40094.0 50 37553.0 51 34992.5 52 32726.5 53 30837.5 54 29409.0 55 28002.0 56 26758.5 57 26122.5 58 25578.0 59 24864.5 60 24188.0 61 23603.5 62 23128.0 63 22760.5 64 22406.5 65 22277.0 66 21866.0 67 21219.5 68 20545.0 69 19577.0 70 18274.5 71 16806.5 72 15104.5 73 13205.0 74 11349.0 75 9375.5 76 7537.0 77 5839.5 78 4385.0 79 3283.0 80 2343.5 81 1585.5 82 1101.5 83 741.5 84 480.0 85 312.0 86 213.5 87 167.5 88 130.0 89 85.0 90 42.5 91 30.5 92 25.0 93 13.5 94 8.0 95 5.0 96 5.0 97 4.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6888598962868124 2 0.0033550128491580816 3 3.246786628217498E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.18834068099185003 14-15 2.7056555235145814E-5 16-17 3.517352180568956E-4 18-19 0.11325874021432039 20-21 5.411311047029163E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.00148811053793302 42-43 0.0 44-45 0.0 46-47 0.0236203727202823 48-49 8.116966570543745E-5 50-51 5.411311047029163E-5 52-53 1.0822622094058326E-4 54-55 0.0019751285321656445 56-57 0.0016504498693438946 58-59 0.10065038547474242 60-61 0.0017045629798141863 62-63 0.001190488430346416 64-65 0.0024080334159279774 66-67 0.004870179942326247 68-69 0.00530308482608858 70-71 0.003679691511979831 72-73 0.03817679943679075 74-75 0.04453508991705001 76-77 0.15625160648296707 78-79 3.517352180568956E-4 80-81 0.11466568108654795 82-83 0.004870179942326247 84-85 0.005411311047029164 86-87 0.007197043692548788 88-89 0.007197043692548788 90-91 0.02153701796717607 92-93 0.010768508983588035 94-95 0.014826992268859909 96-97 0.010362660655060847 98-99 0.011986053969169597 100-101 0.012527185073872514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1847981.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.98018460985904 #Duplication Level Percentage of deduplicated Percentage of total 1 99.21829357584679 98.20645014812504 2 0.6390977977783099 1.2651603601570294 3 0.07609698589009303 0.22596281134965737 4 0.04130847355400143 0.16354881353306167 5 0.012737807655899637 0.0630395276652911 6 0.01063779606959068 0.0631758611286071 7 0.0018275632053026318 0.012662478041292814 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.623393314108749E-4 0.0 5.4113110470291635E-5 2 2.7056555235145815E-4 0.0 5.4113110470291635E-5 3 3.246786628217498E-4 0.0 5.4113110470291635E-5 4 3.787917732920414E-4 0.0 5.4113110470291635E-5 5 4.329048837623331E-4 0.0 5.4113110470291635E-5 6 4.870179942326247E-4 0.0 5.4113110470291635E-5 7 4.870179942326247E-4 0.0 5.4113110470291635E-5 8 7.034704361137912E-4 0.0 5.4113110470291635E-5 9 7.034704361137912E-4 0.0 5.4113110470291635E-5 10-11 7.575835465840828E-4 0.0 5.4113110470291635E-5 12-13 8.928663227598119E-4 0.0 5.4113110470291635E-5 14-15 0.0010010925437003953 0.0 5.4113110470291635E-5 16-17 0.00116343187511127 0.0 5.4113110470291635E-5 18-19 0.001352827761757291 0.0 5.4113110470291635E-5 20-21 0.001461053982697874 0.0 5.4113110470291635E-5 22-23 0.0015963367588736031 0.0 1.0822622094058327E-4 24-25 0.0017316195350493323 0.0 1.0822622094058327E-4 26-27 0.002380976860692832 0.0 1.0822622094058327E-4 28-29 0.0033008997386877893 0.0 1.0822622094058327E-4 30-31 0.004951349608031685 0.0 1.0822622094058327E-4 32-33 0.007251156803019078 0.0 1.0822622094058327E-4 34-35 0.011661375306347846 0.0 1.3528277617572907E-4 36-37 0.01745147812666905 0.0 1.623393314108749E-4 38-39 0.02567667091815338 0.0 1.623393314108749E-4 40-41 0.03739215933497152 0.0 1.623393314108749E-4 42-43 0.053734318696999586 0.0 1.623393314108749E-4 44-45 0.07448669656235643 0.0 1.8939588664602074E-4 46-47 0.10589935719036073 0.0 2.4350899711631234E-4 48-49 0.14569954994126022 0.0 2.7056555235145815E-4 50-51 0.19377904859411432 5.4113110470291635E-5 2.7056555235145815E-4 52-53 0.25195064234967784 5.4113110470291635E-5 3.787917732920414E-4 54-55 0.314316002166689 5.4113110470291635E-5 4.058483285271872E-4 56-57 0.39223888124390893 5.4113110470291635E-5 4.870179942326247E-4 58-59 0.488749613767674 5.4113110470291635E-5 4.870179942326247E-4 60-61 0.6036317472961031 5.4113110470291635E-5 4.870179942326247E-4 62-63 0.7298505774680584 5.4113110470291635E-5 4.870179942326247E-4 64-65 0.8691377238185891 5.4113110470291635E-5 4.870179942326247E-4 66-67 1.0300971709124713 5.4113110470291635E-5 4.870179942326247E-4 68-69 1.2163815537064504 5.4113110470291635E-5 5.140745494677705E-4 70-71 1.4396793040621088 5.4113110470291635E-5 5.411311047029163E-4 72-73 1.672555074971009 8.116966570543745E-5 5.411311047029163E-4 74-75 1.9182556530613681 1.0822622094058327E-4 5.411311047029163E-4 76-77 2.187306038319658 1.0822622094058327E-4 5.411311047029163E-4 78-79 2.497374161314429 1.0822622094058327E-4 5.411311047029163E-4 80-81 2.852680844662364 1.8939588664602074E-4 6.493573256434996E-4 82-83 3.231012656515408 2.1645244188116654E-4 6.493573256434996E-4 84-85 3.6246855351867797 2.1645244188116654E-4 6.493573256434996E-4 86-87 4.05063688425368 2.1645244188116654E-4 6.493573256434996E-4 88-89 4.517822423498943 2.1645244188116654E-4 6.493573256434996E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATCT 1365 0.0 7.4749465 94-95 CTCGTAT 770 2.8194336E-10 7.3973317 90-91 GTCACGT 2390 0.0 7.049084 94-95 CGTATGC 760 1.2556484E-8 6.8689938 92-93 AGTCACG 2405 0.0 6.807791 94-95 TCCGCAT 1530 0.0 6.513736 94-95 GTCCGCA 1755 0.0 6.35467 94-95 ACGTCCG 1970 0.0 5.9021997 92-93 TCCAGTC 4150 0.0 5.889269 94-95 CTCCAGT 4160 0.0 5.875112 94-95 CCGCATC 1945 0.0 5.8559036 94-95 TATGCCG 785 7.186127E-6 5.7432303 94-95 CCAGTCA 3435 0.0 5.7335505 94-95 CACGTCT 6550 0.0 5.723848 94-95 CAGTCAC 3110 0.0 5.7223334 94-95 ACGTCTG 6175 0.0 5.6871805 94-95 TCACGTC 2305 0.0 5.560033 90-91 TCGTATG 860 5.6361678E-6 5.5193267 90-91 GCATCTC 1725 0.0 5.502286 94-95 CGTCTGA 5545 0.0 5.4774785 94-95 >>END_MODULE