FastQCFastQC Report
Thu 11 Sep 2014
NYCLib17_GTAGAG_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib17_GTAGAG_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306031
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA1500.0091542317.673789
AGTCCGG1709.0095616E-411.53223424-25
TCGTGGA4651.1420618E-57.303294790-91
CGTGTAG12850.07.05953394-95
GTCGTGT16500.06.460007792-93
AAGAGCG33100.06.440490294-95
TACTCGG4000.0053678396.3169364-65
TCGTGTA13850.06.058583392-93
GTGTAGG13850.05.894837494-95
CGTGGAG4850.00713842875.61123792-93
CGTCGTG22100.05.54141494-95
TAGATCG16200.05.45969994-95
TCGGAAG38650.05.447709690-91
CGGAAGA38500.05.419337792-93
AGCGTCG25050.05.341501794-95
GTAGGGA11105.6836143E-85.312139594-95
GAAGAGC38500.05.30152694-95
CAGATCG13653.5288394E-105.280778486-87
AGAGCGT29050.05.23055594-95
TGTAGGG14758.367351E-115.227643594-95
Produced by FastQC (version 0.11.2)