FastQCFastQC Report
Thu 11 Sep 2014
NYCLib17_GTAGAG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib17_GTAGAG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306031
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAG5905.1562893E-87.642287794-95
ACGTAGA5601.7442653E-77.62836192-93
AGTCACG8008.367351E-117.416035794-95
GTCACGT7305.5933924E-97.151903694-95
TCACGTA6301.2323762E-66.78258490-91
ACGTCTG20700.06.763998594-95
CGTCTGA18450.06.174355592-93
ACACGTC22300.06.066200792-93
CGTAGAG5150.00128504065.99042994-95
TCTGAAC20850.05.804782494-95
CACGTCT21050.05.75150290-91
CACACGT24600.05.59518894-95
TCGGAAG40350.05.469671294-95
AAGAGCA41600.05.41941194-95
CGGAAGA39450.05.41429992-93
CCAGTCA14202.4920155E-105.347902394-95
GAAGAGC41050.05.318589794-95
ATCGGAA42300.05.163101790-91
GCACACG26250.05.153085794-95
GTCTGAA22200.05.131095494-95
Produced by FastQC (version 0.11.2)