##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib16_CCGTCC_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2422284 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.00899440362897 33.0 22.0 33.0 2.0 33.0 2 25.08601922813345 33.0 15.0 33.0 2.0 33.0 3 25.96085099847912 33.0 22.0 33.0 6.0 33.0 4 28.100238865467468 33.0 22.0 37.0 2.0 37.0 5 28.154298587614004 33.0 22.0 37.0 6.0 37.0 6 29.222133738240437 33.0 27.0 37.0 15.0 37.0 7 28.885839150157455 33.0 27.0 37.0 6.0 37.0 8 28.664306910337515 33.0 27.0 37.0 2.0 37.0 9 29.425521532570087 33.0 27.0 37.0 15.0 37.0 10-11 29.06045265542769 33.0 27.0 37.0 10.5 37.0 12-13 29.3634608906305 33.0 27.0 37.0 10.5 37.0 14-15 29.739315043157617 33.0 27.0 37.0 6.0 40.0 16-17 30.63271792242363 33.0 27.0 37.0 15.0 40.0 18-19 30.960355804686817 33.0 27.0 37.0 15.0 40.0 20-21 30.991978438531568 33.0 27.0 37.0 15.0 40.0 22-23 31.036610281866203 33.0 27.0 37.0 15.0 40.0 24-25 30.63884622942644 33.0 27.0 37.0 10.5 40.0 26-27 30.144402142771035 33.0 27.0 37.0 15.0 40.0 28-29 30.358130590797778 33.0 27.0 37.0 15.0 40.0 30-31 30.286959126180086 33.0 27.0 37.0 15.0 40.0 32-33 30.1478643709821 33.0 27.0 37.0 15.0 40.0 34-35 30.19651700626351 33.0 27.0 37.0 15.0 40.0 36-37 30.442368235929393 33.0 27.0 37.0 15.0 40.0 38-39 30.4902412351318 33.0 27.0 37.0 15.0 40.0 40-41 30.599677411897204 33.0 27.0 37.0 15.0 40.0 42-43 30.528791215233227 33.0 27.0 37.0 15.0 40.0 44-45 30.302432745293284 33.0 27.0 37.0 15.0 40.0 46-47 29.93880341859171 33.0 27.0 37.0 15.0 40.0 48-49 29.797934717811785 33.0 27.0 37.0 15.0 40.0 50-51 29.040205855300204 33.0 24.5 37.0 15.0 38.5 52-53 28.757287130658504 33.0 22.0 37.0 10.5 38.5 54-55 29.724503608990524 33.0 27.0 37.0 15.0 40.0 56-57 29.649778267123096 33.0 27.0 37.0 15.0 40.0 58-59 29.86154327898793 33.0 27.0 37.0 15.0 40.0 60-61 29.62505614535703 33.0 27.0 37.0 15.0 40.0 62-63 29.49720346581986 33.0 27.0 37.0 15.0 40.0 64-65 29.471794182680476 33.0 27.0 37.0 15.0 40.0 66-67 29.352424406056432 33.0 27.0 37.0 15.0 40.0 68-69 29.0725994557203 33.0 27.0 37.0 15.0 40.0 70-71 28.974135980752052 33.0 27.0 37.0 15.0 40.0 72-73 28.732828603087004 33.0 27.0 37.0 15.0 37.0 74-75 28.69088141605196 33.0 27.0 37.0 15.0 37.0 76-77 28.29237446971536 33.0 24.5 37.0 15.0 37.0 78-79 27.712464351826625 33.0 22.0 37.0 10.5 37.0 80-81 27.58232374899062 33.0 22.0 35.0 10.5 37.0 82-83 27.137133589620376 27.0 22.0 33.0 6.0 37.0 84-85 27.227354017943398 27.0 22.0 33.0 15.0 37.0 86-87 26.90482990433822 27.0 22.0 33.0 6.0 37.0 88-89 26.527801240482123 27.0 22.0 33.0 6.0 37.0 90-91 26.38966818093997 27.0 22.0 33.0 6.0 37.0 92-93 26.26141422723347 27.0 22.0 33.0 6.0 37.0 94-95 26.035617830114056 27.0 22.0 33.0 6.0 37.0 96-97 25.887216156321884 27.0 22.0 33.0 6.0 37.0 98-99 25.640373919821126 27.0 22.0 33.0 6.0 37.0 100-101 25.134020205723196 27.0 18.5 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.38569852749352 2306 2 8.53008579844646 2306 3 7.585901191767746 2306 4 9.135125188764565 2306 5 8.938789067496863 2306 6 8.045160012941697 2306 7 8.167079378094694 2306 8 8.693682329794576 2306 9 7.53990523130399 2306 10-11 7.878541728938373 2306 12-13 7.528312359024898 2306 14-15 8.195902629446753 2306 16-17 6.88791504549825 2306 18-19 6.29926447813132 2306 20-21 6.327295448620536 2306 22-23 5.915387504996197 2306 24-25 5.947877230269377 2306 26-27 6.614941776675337 2306 28-29 5.626234769075587 2306 30-31 5.620272465318461 2306 32-33 5.575910130593876 2306 34-35 4.9355257556227805 2306 36-37 4.9769432138993395 2306 38-39 4.728914329016597 2306 40-41 3.921660720812106 2306 42-43 3.6754516353744897 2306 44-45 3.3976479970475033 2306 46-47 3.684245145110669 2306 48-49 3.4972488528730032 2306 50-51 3.3881565054898317 2306 52-53 4.020121116366816 2306 54-55 3.4808456707693587 2306 56-57 3.7171874032641377 2306 58-59 3.3670068961558997 2306 60-61 3.301050733927731 2306 62-63 2.9061902594608675 2306 64-65 2.5370754286509225 2306 66-67 2.4255365096980697 2306 68-69 2.6214894999817098 2306 70-71 2.6105005584545324 2306 72-73 1.8663474031902965 2306 74-75 1.3786763820266543 2306 76-77 1.7722812720900194 2306 78-79 1.989454111368211 2306 80-81 1.6969911977295205 2306 82-83 1.839359052472222 2306 84-85 1.2578698345567219 2306 86-87 1.333975036495282 2306 88-89 1.1841979625220347 2306 90-91 1.120177131023791 2306 92-93 1.0812610513385295 2306 94-95 1.3807800846267213 2306 96-97 1.080836418451991 2306 98-99 1.068855403576535 2306 100-101 0.8860350827921053 2307 1 8.349759575188909 2307 2 8.480461630250037 2307 3 7.605248532616272 2307 4 9.122661072574854 2307 5 8.934539054469198 2307 6 7.482744449151408 2307 7 8.02488226906637 2307 8 8.481938618041227 2307 9 7.532626954529476 2307 10-11 7.701742681018672 2307 12-13 7.2827074987615426 2307 14-15 8.291885894567105 2307 16-17 6.962915781687453 2307 18-19 6.533656382966029 2307 20-21 6.502492382840014 2307 22-23 6.03675419477343 2307 24-25 6.250441698429697 2307 26-27 6.937644844722165 2307 28-29 6.143838261759235 2307 30-31 6.099572521167797 2307 32-33 6.12838308033222 2307 34-35 5.865349584825733 2307 36-37 5.663207886619219 2307 38-39 5.3212249663586775 2307 40-41 4.7536422989043885 2307 42-43 4.5705439527782765 2307 44-45 4.439221304819188 2307 46-47 4.683450060775865 2307 48-49 4.594383550379359 2307 50-51 4.333251767650417 2307 52-53 4.958986034598574 2307 54-55 4.623299868614001 2307 56-57 4.977440786594823 2307 58-59 4.4416977472151515 2307 60-61 4.330225298388818 2307 62-63 4.209461613602944 2307 64-65 4.0367883148633545 2307 66-67 3.7331643536438612 2307 68-69 3.9316909747563322 2307 70-71 3.469689451900095 2307 72-73 2.915174718754333 2307 74-75 2.4867667837506673 2307 76-77 2.674954263199311 2307 78-79 2.9929485200112467 2307 80-81 2.8613583196931174 2307 82-83 2.9835848499275173 2307 84-85 2.22023084456211 2307 86-87 2.154091265612948 2307 88-89 2.2542188118332014 2307 90-91 2.2416419252293167 2307 92-93 2.132411332526715 2307 94-95 2.34692216596639 2307 96-97 2.2622195432922148 2307 98-99 1.958336245118737 2307 100-101 1.5543391388914536 1101 1 8.023440763362089 1101 2 7.747259275469872 1101 3 7.280847377191769 1101 4 8.73096626251468 1101 5 8.597733038544455 1101 6 7.426000070451966 1101 7 7.191775419022388 1101 8 8.090147231395271 1101 9 7.31444422267262 1101 10-11 7.507876766173737 1101 12-13 7.323150862008287 1101 14-15 7.7181027044871975 1101 16-17 6.850540004284486 1101 18-19 6.03472480044762 1101 20-21 5.861716169913709 1101 22-23 5.39966813611565 1101 24-25 5.97484018524235 1101 26-27 6.665306966746513 1101 28-29 5.897773876928227 1101 30-31 5.776676176512648 1101 32-33 5.851221181707757 1101 34-35 5.640557303405604 1101 36-37 5.406709578159401 1101 38-39 5.087253284184705 1101 40-41 4.578855875949891 1101 42-43 4.34630574785913 1101 44-45 4.346767283863503 1101 46-47 4.6978687137780675 1101 48-49 4.487592696721624 1101 50-51 4.0198480461004955 1101 52-53 5.092328328154103 1101 54-55 4.702550078982842 1101 56-57 4.912892058036032 1101 58-59 4.45901059603429 1101 60-61 4.569590494727436 1101 62-63 4.0967457809013474 1101 64-65 3.8409783511550053 1101 66-67 3.6229294673472836 1101 68-69 3.809303516395122 1101 70-71 3.6785469270306166 1101 72-73 3.2385699506680794 1101 74-75 2.6351788238813008 1101 76-77 3.0645548736837327 1101 78-79 3.4784086231995275 1101 80-81 3.047096017245643 1101 82-83 3.2268151836530308 1101 84-85 2.6071280080809913 1101 86-87 2.697545168077866 1101 88-89 2.635989854626871 1101 90-91 2.5073611502614988 1101 92-93 2.283087911893432 1101 94-95 2.524412237775909 1101 96-97 2.396555959000711 1101 98-99 2.2090615606059245 1101 100-101 1.8693012086301017 2201 1 7.077438391669812 2201 2 7.894949363288827 2201 3 6.411956479643528 2201 4 8.448227004004831 2201 5 4.073284466212904 2201 6 5.798627793083757 2201 7 6.6615378904473666 2201 8 7.805225315723952 2201 9 6.481894395481824 2201 10-11 4.2066176684672065 2201 12-13 6.502911278703753 2201 14-15 7.062190141981507 2201 16-17 5.981402410197678 2201 18-19 2.079977131367805 2201 20-21 5.101420878561353 2201 22-23 5.155282661486957 2201 24-25 5.479357531121515 2201 26-27 6.1917109044559595 2201 28-29 5.103032123258053 2201 30-31 5.288066122711907 2201 32-33 5.3892993369845 2201 34-35 4.894922221525089 2201 36-37 4.978490991794885 2201 38-39 4.516239804974781 2201 40-41 4.200981221821678 2201 42-43 4.1671779298620955 2201 44-45 3.9206210995330757 2201 46-47 4.576441994370267 2201 48-49 4.280643159220052 2201 50-51 3.8708370896930084 2201 52-53 4.7083358689192885 2201 54-55 4.277409495863498 2201 56-57 4.393985174139313 2201 58-59 4.097521792467347 2201 60-61 4.0785187459683385 2201 62-63 3.8851275662309455 2201 64-65 3.8146843552957925 2201 66-67 3.461420872035937 2201 68-69 3.7181057196548615 2201 70-71 3.3976292829853243 2201 72-73 2.7517363994228496 2201 74-75 2.404293309171557 2201 76-77 2.8245125075567294 2201 78-79 3.0877943880702894 2201 80-81 2.6957882602270864 2201 82-83 2.8510269737462863 2201 84-85 2.483283415659166 2201 86-87 2.4840433469241034 2201 88-89 2.4215735774834783 2201 90-91 2.220885490513652 2201 92-93 2.1297638110218777 2201 94-95 2.2628778063140373 2201 96-97 2.162670391770977 2201 98-99 1.9888939204765919 2201 100-101 1.7797651075853231 2304 1 2.0704759987404024 2304 2 5.1620759398606815 2304 3 5.8802906575429255 2304 4 4.491982282542718 2304 5 6.751226660616865 2304 6 6.452262560781488 2304 7 7.097060548698661 2304 8 6.589728355579318 2304 9 5.8614303151676665 2304 10-11 6.849706613505177 2304 12-13 6.814913622520184 2304 14-15 7.004493745424536 2304 16-17 5.614264999881858 2304 18-19 5.3577938174303625 2304 20-21 5.179008495009544 2304 22-23 4.81846295944986 2304 24-25 4.9754553796331535 2304 26-27 5.9165298702644336 2304 28-29 5.23918907974484 2304 30-31 5.171892764169737 2304 32-33 5.360127978607743 2304 34-35 4.836393210972094 2304 36-37 5.0361221296059355 2304 38-39 4.693573049774294 2304 40-41 4.0453608501893115 2304 42-43 3.545233540751287 2304 44-45 3.5129968463915766 2304 46-47 4.434637791576559 2304 48-49 4.125189021487049 2304 50-51 3.6292171770386155 2304 52-53 4.3832316378180565 2304 54-55 4.141306801290039 2304 56-57 4.61127762348573 2304 58-59 3.890306798565703 2304 60-61 3.979175224256 2304 62-63 3.636107367705499 2304 64-65 3.430957337685715 2304 66-67 3.2743200867642344 2304 68-69 3.603202495276374 2304 70-71 3.5129676510973056 2304 72-73 3.1897659394313997 2304 74-75 2.3936134742638373 2304 76-77 2.8136850308521915 2304 78-79 3.2585267142149092 2304 80-81 2.932706677475334 2304 82-83 2.9853948480841765 2304 84-85 2.829628471569439 2304 86-87 2.477883792668596 2304 88-89 2.6422089474441854 2304 90-91 2.574976836449075 2304 92-93 2.349983770971974 2304 94-95 2.612792965284669 2304 96-97 2.5691535837414214 2304 98-99 2.3368902486528285 2304 100-101 1.8950361200977142 1102 1 6.9916569886105115 1102 2 7.441228844865197 1102 3 6.022347474209795 1102 4 5.990430974232311 1102 5 6.412796392803045 1102 6 5.786190607574834 1102 7 6.572726698123251 1102 8 6.396542305986763 1102 9 4.829340497676249 1102 10-11 5.8998607674549675 1102 12-13 5.846869889375302 1102 14-15 6.05082683100267 1102 16-17 5.20480337219502 1102 18-19 5.263944679656049 1102 20-21 5.366303349948421 1102 22-23 5.053179391198356 1102 24-25 1.7711705486451024 1102 26-27 5.342414778399984 1102 28-29 4.767593887003564 1102 30-31 4.684118459050961 1102 32-33 4.825194835942121 1102 34-35 4.828859210280857 1102 36-37 4.757834652575703 1102 38-39 3.8009594107986757 1102 40-41 3.8140613087279718 1102 42-43 4.050447004452469 1102 44-45 -3.837461415390859 1102 46-47 1.4634831618281332 1102 48-49 -0.7697565427198576 1102 50-51 2.545761507178085 1102 52-53 -0.44192252657967046 1102 54-55 2.3674408089186976 1102 56-57 -0.8585491530067664 1102 58-59 2.8179291970839344 1102 60-61 3.7817735664206182 1102 62-63 3.4250972578727037 1102 64-65 3.098483302875728 1102 66-67 3.015066226568372 1102 68-69 3.3185065728100263 1102 70-71 3.1800076309286247 1102 72-73 2.6889933674148807 1102 74-75 2.439481627057674 1102 76-77 2.5730165975105947 1102 78-79 -1.652298106843432 1102 80-81 1.5028172875967023 1102 82-83 2.597799740597985 1102 84-85 2.2358008304064434 1102 86-87 2.2112596246893226 1102 88-89 2.2506012610709156 1102 90-91 2.055426909145755 1102 92-93 2.0279760783717364 1102 94-95 2.4216245933768903 1102 96-97 2.313327714755932 1102 98-99 2.2540092931773046 1102 100-101 2.025078028825451 2202 1 -20.732996390938883 2202 2 -7.76818216931415 2202 3 -0.6891354450236307 2202 4 1.5818724029430946 2202 5 1.3301386517801248 2202 6 1.7767331889216962 2202 7 3.947627439044979 2202 8 2.741276720146235 2202 9 3.3447040024265533 2202 10-11 2.15766707800757 2202 12-13 1.5490029111270616 2202 14-15 2.5574644827174993 2202 16-17 1.5972575284039614 2202 18-19 1.7599102710578194 2202 20-21 1.243132919262031 2202 22-23 1.4587305035837943 2202 24-25 2.3143350283451944 2202 26-27 3.520631889343143 2202 28-29 -0.9392501233093569 2202 30-31 1.022757271789306 2202 32-33 1.576365716998069 2202 34-35 1.595109892900691 2202 36-37 2.9907116638540856 2202 38-39 2.489298098728135 2202 40-41 1.903727754920201 2202 42-43 0.7930230863650287 2202 44-45 0.7985315560179664 2202 46-47 -1.2650524898447522 2202 48-49 0.9114160044299169 2202 50-51 1.7659016755834784 2202 52-53 1.8538626321026612 2202 54-55 -0.7918306955435135 2202 56-57 1.1094625862310892 2202 58-59 1.9732396831274954 2202 60-61 2.1579477523138415 2202 62-63 -2.051498796197169 2202 64-65 0.06105049363640447 2202 66-67 0.6340387161911849 2202 68-69 1.5792217099676122 2202 70-71 -2.4786266667182417 2202 72-73 -4.342720420913256 2202 74-75 -1.0810799878727124 2202 76-77 -0.1214286319909803 2202 78-79 1.5770439821054651 2202 80-81 1.6330495287686837 2202 82-83 -0.8224369290559501 2202 84-85 -0.30921361743046205 2202 86-87 0.7133497633283881 2202 88-89 1.3106165932366949 2202 90-91 1.382515372574197 2202 92-93 1.3390621208359335 2202 94-95 1.6811659435009219 2202 96-97 1.30940521478556 2202 98-99 1.0974615444324556 2202 100-101 1.0455363391008987 2305 1 5.585872937104757 2305 2 6.952800353489241 2305 3 6.734904429587889 2305 4 7.741079329804901 2305 5 7.8477518254748375 2305 6 6.11405757468027 2305 7 3.1351366427696945 2305 8 5.99418959711743 2305 9 6.255034060875175 2305 10-11 4.632294601882471 2305 12-13 5.101912801167575 2305 14-15 5.550046199075691 2305 16-17 4.966490290259632 2305 18-19 4.406185190244386 2305 20-21 5.062599176923538 2305 22-23 5.010253068671162 2305 24-25 5.159042904568352 2305 26-27 6.016818237556912 2305 28-29 5.11494845123341 2305 30-31 5.3157763778413845 2305 32-33 5.40846541970064 2305 34-35 5.161810370144384 2305 36-37 4.645771387467782 2305 38-39 4.4854437516418315 2305 40-41 4.014950683904424 2305 42-43 3.9155666456620573 2305 44-45 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-26.64076502594309 1209 40-41 -23.499017185568032 1209 42-43 -23.89759087621106 1209 44-45 -26.810490011578036 1209 46-47 -20.10055115740549 1209 48-49 -20.190836906607842 1209 50-51 -22.291482812626892 1209 52-53 -24.964127899196654 1209 54-55 -22.088147652302347 1209 56-57 -25.861473763058758 1209 58-59 -26.148275308981923 1209 60-61 -25.903993331326348 1209 62-63 -26.055838617343934 1209 64-65 -25.94473593455928 1209 66-67 -13.881148008359034 1209 68-69 -25.625275922857217 1209 70-71 -25.48691177981661 1209 72-73 -15.645034317782649 1209 74-75 -16.73886324441978 1209 76-77 -14.023876832571741 1209 78-79 -14.741504863402875 1209 80-81 -24.504115121415428 1209 82-83 -16.17912166988062 1209 84-85 -13.556735164794198 1209 86-87 -13.488862105111785 1209 88-89 -11.194652467003435 1209 90-91 -13.702903329362126 1209 92-93 -17.690438450484734 1209 94-95 -23.108813014942 1209 96-97 -18.8168311212088 1209 98-99 -10.22029968784745 1209 100-101 -5.667389959569746 1212 1 -20.732996390938883 1212 2 -20.565920201928563 1212 3 -21.47635269910043 1212 4 -23.487038696478262 1212 5 -23.63724693475328 1212 6 -24.740889115128805 1212 7 -24.29382442839321 1212 8 -23.947045215677015 1212 9 -24.870182899204167 1212 10-11 -24.594831606895113 1212 12-13 -24.886557320330066 1212 14-15 -25.247375075409803 1212 16-17 -26.30700338690377 1212 18-19 -20.907959327449053 1212 20-21 -26.733458348491784 1212 22-23 -26.957760816773913 1212 24-25 -23.107324952073427 1212 26-27 -25.719110351582685 1212 28-29 -20.56862293889059 1212 30-31 -15.648467306895949 1212 32-33 -25.906932547073474 1212 34-35 -22.186300808715426 1212 36-37 -26.364890650717193 1212 38-39 -26.64076502594309 1212 40-41 -21.231204037861644 1212 42-43 -17.39297301483163 1212 44-45 -14.271344104104728 1212 46-47 -17.711321170310736 1212 48-49 -20.587122412323957 1212 50-51 -12.647367734348112 1212 52-53 -5.457603581284438 1212 54-55 -4.133837431088207 1212 56-57 -4.046527143841676 1212 58-59 -2.988726080037676 1212 60-61 -14.418228206771188 1212 62-63 -12.364854038459 1212 64-65 -16.746633917951925 1212 66-67 -12.888666290788347 1212 68-69 -8.28008019331985 1212 70-71 -15.541478802355162 1212 72-73 -12.069502791183508 1212 74-75 -8.308460314016848 1212 76-77 -19.591586999036355 1212 78-79 -10.322165898690244 1212 80-81 -8.003522001605226 1212 82-83 -5.3989181841611185 1212 84-85 -4.823570642483766 1212 86-87 -3.4788637997398126 1212 88-89 -3.2150434046541605 1212 90-91 -3.619824190233164 1212 92-93 -3.512909869931242 1212 94-95 -3.2713278429372536 1212 96-97 -13.351339613902345 1212 98-99 -10.711585389097564 1212 100-101 -6.798534615625432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 403.0 3 877.0 4 379.0 5 2372.0 6 3063.0 7 2540.0 8 5481.0 9 10276.0 10 12788.0 11 15500.0 12 20879.0 13 27529.0 14 31663.0 15 33680.0 16 35959.0 17 39759.0 18 45841.0 19 50965.0 20 55986.0 21 63031.0 22 71417.0 23 77741.0 24 82917.0 25 86207.0 26 91676.0 27 96557.0 28 99115.0 29 100197.0 30 104892.0 31 110236.0 32 121631.0 33 141496.0 34 162467.0 35 180504.0 36 191464.0 37 177599.0 38 66966.0 39 231.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.113847684796465 29.582026643319676 19.083917606167816 24.220208065716044 2 23.729223305070395 30.44926985470408 25.489265325951838 20.33224151427369 3 19.572731823615868 28.353353433437643 29.13945190261431 22.934462840332177 4 22.04242357116386 28.198556781682033 27.97699412235063 21.782025524803476 5 21.664664935669027 28.790857570631427 27.699702193303622 21.844775300395924 6 21.391225109980425 29.33628067788925 27.337885149481817 21.934609062648512 7 21.695796408576758 28.933978580973484 28.2362458806926 21.133979129757154 8 20.963663643953556 28.45221085523794 29.132057389475598 21.452068111332903 9 21.101372350455705 27.50039284841289 29.648531619932257 21.74970318119915 10-11 21.911515464076576 27.863504426651154 28.280273026410036 21.944707082862237 12-13 21.769908492802482 28.2150282798187 28.013196303412013 22.001866923966805 14-15 21.842498966382482 28.32784743805423 27.939572581335003 21.89008101422828 16-17 22.091169927835118 28.279472982171903 27.79230170561902 21.83705538437396 18-19 22.154197662672725 28.155948751992128 27.701819859295224 21.988033726039923 20-21 22.170579883683335 28.275436975545215 27.640074341112907 21.91390879965854 22-23 21.972174683992133 28.243867923523002 27.883477226760554 21.90048016572431 24-25 21.930802521001212 28.203294653567685 27.96380638327364 21.902096442157465 26-27 21.84589197725292 28.353964774815978 27.906789606758544 21.89335364117256 28-29 22.145416155894306 28.22420939160185 27.85991912222492 21.77045533027892 30-31 22.098126293648328 28.17489249113357 27.73115382820667 21.99582738701144 32-33 21.870432236662158 28.329407908250413 27.865040586376562 21.935119268710867 34-35 22.033308637493004 28.196640663729845 27.889866099125648 21.880184599651507 36-37 22.006483519245045 28.335155607443397 27.74458120137201 21.913779671939547 38-39 22.029902592924156 28.230176832240765 27.907691322320368 21.832229252514715 40-41 22.133182818318478 28.209955081458315 27.744126980547275 21.912735119675933 42-43 21.98245489868718 28.270788238592953 27.812287714423352 21.934469148296518 44-45 21.90684073037162 28.18554731447318 28.02141110714253 21.886200848012674 46-47 22.01888044131096 28.31007284728021 27.811400672597408 21.859646038811423 48-49 21.98530068128125 28.21613187200049 27.922183127078636 21.876384319639627 50-51 22.12582541210318 28.19871960208269 27.737222756944576 21.938232228869555 52-53 21.97798477086356 28.2329855146928 27.946584808065605 21.842444906378034 54-55 21.98703256695238 28.166002645437043 27.899546526529395 21.947418261081182 56-57 21.929529545352313 28.289287547520725 27.940273233393548 21.840909673733414 58-59 22.0853343463134 28.276126793574285 27.81267583547795 21.82586302463437 60-61 22.08249882279077 28.106712708627633 27.749155025375398 22.061633443206194 62-63 22.048068047426366 28.27672924629293 27.804168444791937 21.871034261488767 64-65 22.11787331943346 28.21416449446318 27.800366000264177 21.867596185839183 66-67 22.145537579983525 28.23378156140956 27.738670916541068 21.882009942065846 68-69 22.15065119234423 28.248669382204696 27.81697539995514 21.783704025495933 70-71 22.356689889804752 28.202208074314044 27.595091871067805 21.8460101648134 72-73 22.184354350725076 28.24996926979877 27.752997356686222 21.81267902278993 74-75 22.228356828853773 28.209481204444604 27.710036521802017 21.852125444899603 76-77 22.382510166856083 28.3274021057154 27.544015597646087 21.746072129782434 78-79 22.418032343195296 28.19948884460393 27.62910254669977 21.75337626550101 80-81 22.517908733566237 28.23218380144595 27.472444439660133 21.77746302532768 82-83 22.501795209772414 28.21592561140353 27.536868452878778 21.745410725945277 84-85 22.652429767361163 28.103052623099465 27.40965958264225 21.834858026897123 86-87 22.629688178932486 28.24805433381427 27.473920211311942 21.648337275941305 88-89 22.77376584405097 28.194472820752882 27.442411702281717 21.589349632914434 90-91 22.950704519244795 28.149346262500718 27.148193859829988 21.7517553584245 92-93 22.974613113711467 28.16449061436587 27.194581793114516 21.666314478808147 94-95 23.132292198204514 28.12147189322888 27.0896603832111 21.65657552535551 96-97 23.199996850922897 28.16594641886745 27.04678099059607 21.587275739613588 98-99 23.344579326595376 28.223387810373303 26.98247293980206 21.449559923229263 100-101 23.80704417723525 28.106017254812393 26.580653276415873 21.506285291536486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 179.0 1 176.0 2 200.0 3 214.0 4 206.0 5 242.0 6 306.5 7 349.5 8 443.5 9 599.0 10 780.5 11 1077.0 12 1503.5 13 2047.0 14 2611.5 15 3252.5 16 4183.5 17 5215.0 18 6608.0 19 8417.5 20 10578.5 21 13302.5 22 16451.0 23 20099.0 24 24477.0 25 29101.5 26 33902.0 27 39104.0 28 44609.5 29 50194.0 30 55246.5 31 60220.5 32 64900.0 33 69106.0 34 73363.0 35 77032.5 36 80011.0 37 81933.0 38 82141.5 39 81005.0 40 78778.0 41 75758.5 42 71936.5 43 67595.5 44 63255.0 45 59706.5 46 56796.5 47 54108.0 48 51577.5 49 49267.0 50 47267.5 51 44953.5 52 42826.5 53 41664.0 54 40746.5 55 39675.5 56 39377.0 57 38986.5 58 38230.0 59 37383.5 60 36106.0 61 34386.0 62 32233.5 63 30094.5 64 27627.5 65 25106.0 66 22576.0 67 20001.5 68 17636.5 69 15188.0 70 13046.5 71 11284.5 72 9593.0 73 7975.5 74 6613.0 75 5476.5 76 4422.5 77 3541.5 78 2865.0 79 2308.0 80 1818.0 81 1455.5 82 1191.5 83 964.0 84 782.0 85 642.0 86 531.5 87 417.0 88 310.0 89 238.0 90 191.5 91 134.0 92 97.0 93 80.0 94 61.0 95 42.5 96 25.0 97 14.5 98 8.5 99 5.5 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.709333835338878 2 11.029466404434823 3 9.697417808976983 4 11.661844771298494 5 9.117675714325818 6 5.312919542052047 7 9.727595938378819 8 10.05484905981297 9 5.421494754537453 10-11 6.34273272663321 12-13 5.378333011323198 14-15 7.337702763177233 16-17 2.66232200683322 18-19 3.0798411746929757 20-21 3.084980951861962 22-23 3.1465757111882833 24-25 3.5008281440161437 26-27 4.319229289381426 28-29 1.7298549633321276 30-31 2.238527769658719 32-33 3.025305868345743 34-35 1.984284254034622 36-37 3.074990380979274 38-39 1.3827651918602442 40-41 1.1492665599904883 42-43 0.5464057889165762 44-45 1.2914257783150118 46-47 1.363960625591384 48-49 1.2608141737302478 50-51 1.5633592097375866 52-53 2.456792845099914 54-55 1.7790234340812223 56-57 1.799334842652637 58-59 1.5374745488142596 60-61 1.368914627681973 62-63 0.6007346785100344 64-65 1.2358583881989065 66-67 0.5543734756122733 68-69 0.7875624823513675 70-71 0.9831217148773638 72-73 0.08366071030481975 74-75 0.1030638851596262 76-77 0.44976146479933815 78-79 0.8531617267009153 80-81 0.2882403549707631 82-83 0.5118516243347188 84-85 0.06518641084199871 86-87 0.09720164935242936 88-89 0.07990392538612318 90-91 0.08764455365266831 92-93 0.1678993875202082 94-95 0.44773858061234767 96-97 0.3665342296774449 98-99 0.06481486068520455 100-101 0.22503554496499997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2422284.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.08840370605903 #Duplication Level Percentage of deduplicated Percentage of total 1 98.2208723649152 96.34328580891098 2 1.6401477295706746 3.2175894527140882 3 0.11080243259822588 0.3260530122092452 4 0.02561203957322963 0.10048976309578032 5 0.0025654333426829725 0.012581963069903593 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.0641675377453675E-4 0.0 0.0 2 2.889834552843515E-4 0.0 0.0 3 3.7155015679416614E-4 0.0 0.0 4 4.954002090588883E-4 0.0 0.0 5 7.018169628334249E-4 0.0 0.0 6 7.431003135883323E-4 0.0 0.0 7 9.082337166079617E-4 0.0 0.0 8 0.001155933821137406 0.0 0.0 9 0.0012385005226472207 0.0 0.0 10-11 0.0014242756010443038 4.1283350754907355E-5 0.0 12-13 0.001775184082461016 4.1283350754907355E-5 0.0 14-15 0.002105450888500275 4.1283350754907355E-5 0.0 16-17 0.0024150760191620803 4.1283350754907355E-5 0.0 18-19 0.0027247011498238854 8.256670150981471E-5 0.0 20-21 0.0030136846051082365 8.256670150981471E-5 0.0 22-23 0.003364593086524949 8.256670150981471E-5 4.1283350754907355E-5 24-25 0.0037361432433191157 8.256670150981471E-5 4.1283350754907355E-5 26-27 0.004252185127755458 1.2385005226472207E-4 4.1283350754907355E-5 28-29 0.0047682270121917995 1.6513340301962942E-4 4.1283350754907355E-5 30-31 0.005655819053422308 2.0641675377453675E-4 4.1283350754907355E-5 32-33 0.006997527952956796 2.0641675377453675E-4 4.1283350754907355E-5 34-35 0.009164903867589433 2.0641675377453675E-4 4.1283350754907355E-5 36-37 0.012570780304869288 2.0641675377453675E-4 4.1283350754907355E-5 38-39 0.016905532134134562 2.0641675377453675E-4 4.1283350754907355E-5 40-41 0.02297418469510594 2.0641675377453675E-4 4.1283350754907355E-5 42-43 0.030879946364670702 2.0641675377453675E-4 1.2385005226472207E-4 44-45 0.04107693400113281 2.0641675377453675E-4 1.2385005226472207E-4 46-47 0.0541843978658159 2.0641675377453675E-4 1.4449172764217574E-4 48-49 0.07111057167532792 2.0641675377453675E-4 2.0641675377453675E-4 50-51 0.09383705626590441 2.0641675377453675E-4 2.0641675377453675E-4 52-53 0.12197165980537378 2.0641675377453675E-4 2.0641675377453675E-4 54-55 0.15634004930883416 2.0641675377453675E-4 2.0641675377453675E-4 56-57 0.19718992488081497 2.0641675377453675E-4 2.0641675377453675E-4 58-59 0.24902117175360117 2.0641675377453675E-4 2.0641675377453675E-4 60-61 0.3120608483563447 2.0641675377453675E-4 2.4770010452944413E-4 62-63 0.3786096097732553 2.0641675377453675E-4 2.4770010452944413E-4 64-65 0.45677963442767244 2.0641675377453675E-4 2.889834552843515E-4 66-67 0.5460342387597821 2.0641675377453675E-4 2.889834552843515E-4 68-69 0.6531232506180118 2.0641675377453675E-4 2.889834552843515E-4 70-71 0.7801727625662391 2.0641675377453675E-4 2.889834552843515E-4 72-73 0.9078828081265451 2.0641675377453675E-4 3.3026680603925884E-4 74-75 1.0465742249876562 2.0641675377453675E-4 3.3026680603925884E-4 76-77 1.2015519237215786 2.0641675377453675E-4 3.3026680603925884E-4 78-79 1.3754373971012481 2.0641675377453675E-4 3.3026680603925884E-4 80-81 1.5832371431260743 2.2705842915199045E-4 3.509084814167125E-4 82-83 1.807529587777486 2.4770010452944413E-4 3.7155015679416614E-4 84-85 2.0387782770310996 2.4770010452944413E-4 4.128335075490735E-4 86-87 2.2898016912963137 2.4770010452944413E-4 4.334751829265272E-4 88-89 2.568072117059767 2.889834552843515E-4 4.5411685830398085E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACCGT 280 0.007070326 7.746182 18-19 GTGTAGG 2935 0.0 5.7968407 94-95 CGTGTAG 2785 0.0 5.7017875 94-95 CGTCGTG 5400 0.0 5.671242 94-95 TCGTGTA 2920 0.0 5.671124 92-93 GTAGGGA 2100 0.0 5.6172304 94-95 TGTAGGG 2845 0.0 5.581539 94-95 GTCGTGT 3505 0.0 5.436626 94-95 GCGTCGT 5655 0.0 5.3352804 94-95 GGTTCCG 560 0.005281412 5.318356 78-79 AGCGTCG 6070 0.0 5.119895 92-93 >>END_MODULE