FastQCFastQC Report
Thu 11 Sep 2014
NYCLib16_CCGTCC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib16_CCGTCC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422284
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7250.010.47716444-45
CTCGTAT8450.010.39385842-43
TCGTATG8950.010.34363942-43
TATGCCG7400.09.94401546-47
TCTCGTA9500.09.24296440-41
GCCGTCT9250.08.72110848-49
GTATGCC9950.08.349812544-45
CGTCTTC11700.07.50342250-51
ATGCCGT11400.07.079552746-47
CCGTCTT10850.06.997834750-51
ATCTCGT12950.06.78055340-41
CATCTCG14350.06.779012738-39
ACCCGTC19200.06.550258694-95
CGTCCAT15450.05.98922534-35
CGTCTGA46650.05.951384594-95
CCCGTCC18650.05.85188232-33
CACACGT60150.05.641364694-95
TCACCCG22550.05.57744892-93
CCGTCCA16750.05.524389334-35
TGCCGTC13851.2732926E-105.481945548-49
Produced by FastQC (version 0.11.2)