##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib15_ATGTCA_L007_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1342757
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.90601352292336	27.0	22.0	33.0	2.0	33.0
2	24.960791118571716	33.0	15.0	33.0	2.0	33.0
3	25.8225300631462	33.0	22.0	33.0	6.0	33.0
4	27.947292026777742	33.0	22.0	37.0	2.0	37.0
5	28.00267136942872	33.0	22.0	37.0	6.0	37.0
6	29.05050429824607	33.0	27.0	37.0	15.0	37.0
7	28.717143906157258	33.0	27.0	37.0	6.0	37.0
8	28.47120886355461	33.0	27.0	37.0	2.0	37.0
9	29.22374711135373	33.0	27.0	37.0	15.0	37.0
10-11	28.847159612647708	33.0	24.5	37.0	10.5	37.0
12-13	29.146744720005184	33.0	27.0	37.0	10.5	37.0
14-15	29.49139010260233	33.0	27.0	37.0	6.0	40.0
16-17	30.345970641002058	33.0	27.0	37.0	15.0	40.0
18-19	30.654029731366137	33.0	27.0	37.0	15.0	40.0
20-21	30.64942465390238	33.0	27.0	37.0	15.0	40.0
22-23	30.67551761040903	33.0	27.0	37.0	15.0	40.0
24-25	30.26128405958785	33.0	27.0	37.0	10.5	40.0
26-27	29.74466638416333	33.0	27.0	37.0	10.5	40.0
28-29	29.92688922865418	33.0	27.0	37.0	15.0	40.0
30-31	29.827290045778945	33.0	27.0	37.0	15.0	40.0
32-33	29.65997794090815	33.0	27.0	37.0	15.0	40.0
34-35	29.689159319221574	33.0	27.0	37.0	15.0	40.0
36-37	29.93210573469362	33.0	27.0	37.0	15.0	40.0
38-39	29.96631780731733	33.0	27.0	37.0	15.0	40.0
40-41	30.03680301052238	33.0	27.0	37.0	15.0	40.0
42-43	29.929506232326474	33.0	27.0	37.0	15.0	40.0
44-45	29.661879997646636	33.0	27.0	37.0	15.0	40.0
46-47	29.274656546195626	33.0	22.0	37.0	15.0	40.0
48-49	29.109895908194858	33.0	22.0	37.0	10.5	40.0
50-51	28.32557380076961	33.0	22.0	37.0	10.5	38.5
52-53	28.024426981203597	33.0	22.0	37.0	10.5	37.0
54-55	28.952093714648292	33.0	22.0	37.0	15.0	40.0
56-57	28.868671695623256	33.0	22.0	37.0	10.5	40.0
58-59	29.04353468274602	33.0	22.0	37.0	15.0	40.0
60-61	28.78895064408527	33.0	22.0	37.0	10.5	40.0
62-63	28.64643975045373	33.0	22.0	37.0	15.0	40.0
64-65	28.603208547786384	33.0	22.0	37.0	10.5	40.0
66-67	28.477167871774267	33.0	22.0	37.0	15.0	40.0
68-69	28.179038351689847	33.0	22.0	37.0	10.5	38.5
70-71	28.077665206735098	33.0	22.0	37.0	10.5	37.0
72-73	27.841015909803488	33.0	22.0	37.0	15.0	37.0
74-75	27.789035544033656	33.0	22.0	37.0	15.0	37.0
76-77	27.39316160705176	30.0	22.0	35.0	6.0	37.0
78-79	26.82698805517305	27.0	22.0	33.0	6.0	37.0
80-81	26.70767197638888	27.0	22.0	33.0	6.0	37.0
82-83	26.285069078023795	27.0	22.0	33.0	6.0	37.0
84-85	26.380843667171348	27.0	22.0	33.0	6.0	37.0
86-87	26.08972062703825	27.0	22.0	33.0	6.0	37.0
88-89	25.725238818341666	27.0	22.0	33.0	6.0	37.0
90-91	25.590512281820168	27.0	22.0	33.0	6.0	37.0
92-93	25.476490534028123	27.0	22.0	33.0	6.0	37.0
94-95	25.250908764579144	27.0	22.0	33.0	6.0	37.0
96-97	25.12506023055549	27.0	18.5	33.0	6.0	37.0
98-99	24.892433627231135	27.0	15.0	33.0	6.0	37.0
100-101	24.40219228050943	27.0	15.0	33.0	6.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	8.403223535431653
2306	2	8.56580848937205
2306	3	7.651608169823721
2306	4	9.195907407974026
2306	5	9.134863796372539
2306	6	8.098274367237192
2306	7	8.280016562946525
2306	8	8.823591065703859
2306	9	7.773630034069651
2306	10-11	8.130318644910076
2306	12-13	7.742665584856429
2306	14-15	8.273308196007147
2306	16-17	7.078713361202368
2306	18-19	6.438092009312189
2306	20-21	6.4197365626931955
2306	22-23	5.9834411972193635
2306	24-25	6.134794780266439
2306	26-27	6.749698972015462
2306	28-29	5.7477291583576005
2306	30-31	5.592422783676753
2306	32-33	5.678299512747358
2306	34-35	5.1413081369481155
2306	36-37	5.11746456579759
2306	38-39	4.641788814381613
2306	40-41	3.682015432636387
2306	42-43	3.5159070416620715
2306	44-45	3.3210666109211893
2306	46-47	3.5486279589174146
2306	48-49	3.396470477652855
2306	50-51	3.256604968869091
2306	52-53	3.885079047172237
2306	54-55	3.4778668170245126
2306	56-57	3.8972275371364837
2306	58-59	3.20701620456256
2306	60-61	3.253257634631055
2306	62-63	2.8195751063672674
2306	64-65	2.2752808535059224
2306	66-67	2.188345433785198
2306	68-69	2.4141697341242576
2306	70-71	2.3901008943307893
2306	72-73	1.913359261234742
2306	74-75	1.52689548284512
2306	76-77	1.8904156859572367
2306	78-79	2.111631272323134
2306	80-81	1.652496756598385
2306	82-83	1.8106794516358242
2306	84-85	1.5144853005925079
2306	86-87	1.313533274399667
2306	88-89	1.3233203427569187
2306	90-91	1.1291568548537292
2306	92-93	1.2822248571992283
2306	94-95	1.3528481775373713
2306	96-97	1.0791448323066817
2306	98-99	0.8727867553581952
2306	100-101	0.7557017601228573
2307	1	8.335398391906498
2307	2	8.659203576947238
2307	3	7.647104360148742
2307	4	9.20634686412096
2307	5	9.158671262365235
2307	6	7.837813833887406
2307	7	8.366443809858776
2307	8	8.73132637559997
2307	9	7.523105458993125
2307	10-11	7.942109232184528
2307	12-13	7.421175657404394
2307	14-15	8.389500810084268
2307	16-17	7.10466446114976
2307	18-19	6.660486466690788
2307	20-21	6.708099662374941
2307	22-23	6.283372325398432
2307	24-25	6.283926882282927
2307	26-27	7.0345391173280625
2307	28-29	6.268741952101976
2307	30-31	6.047544826568739
2307	32-33	6.2166420062252
2307	34-35	5.928056063492999
2307	36-37	5.852279442016709
2307	38-39	5.380829610897351
2307	40-41	4.875224204803356
2307	42-43	4.6357785107596
2307	44-45	4.543058246450901
2307	46-47	4.952842492814504
2307	48-49	4.722015823782911
2307	50-51	4.382436223134576
2307	52-53	4.991744059062604
2307	54-55	4.538332837512023
2307	56-57	4.813397260664811
2307	58-59	4.406796942778048
2307	60-61	4.4754291917582805
2307	62-63	4.226642834131759
2307	64-65	4.033259104658573
2307	66-67	3.800722485397735
2307	68-69	3.889641227619528
2307	70-71	3.410428325733328
2307	72-73	2.8988236450009133
2307	74-75	2.584624410705505
2307	76-77	2.750018436025883
2307	78-79	3.1574396878497097
2307	80-81	2.989123707063893
2307	82-83	3.102438924719884
2307	84-85	2.2758534489119597
2307	86-87	2.32800495252652
2307	88-89	2.45670029713596
2307	90-91	2.2997400151342013
2307	92-93	2.1924221963377803
2307	94-95	2.1895077446799185
2307	96-97	2.136296727582895
2307	98-99	1.8770365788513352
2307	100-101	1.535314111505027
1101	1	8.029229170617914
1101	2	7.817437984830576
1101	3	7.271299672126862
1101	4	8.741397067294116
1101	5	8.593844556330854
1101	6	7.3577710182228095
1101	7	7.180462509038964
1101	8	8.016250948088903
1101	9	7.180862500585395
1101	10-11	7.4388624139899875
1101	12-13	7.219224074894232
1101	14-15	7.700134906488376
1101	16-17	6.897526286309912
1101	18-19	6.074528406789835
1101	20-21	5.71269401741398
1101	22-23	5.293916876979026
1101	24-25	5.946255062242564
1101	26-27	6.523470705495097
1101	28-29	5.884881442714871
1101	30-31	5.711837027840197
1101	32-33	5.634743590499362
1101	34-35	5.312480925917551
1101	36-37	5.259975956594175
1101	38-39	4.845174804373457
1101	40-41	4.531830778479684
1101	42-43	4.162269763651224
1101	44-45	3.9211659313147145
1101	46-47	4.170483029147501
1101	48-49	3.900643077148814
1101	50-51	3.56790081661914
1101	52-53	4.511109309197089
1101	54-55	4.193087593686656
1101	56-57	4.363530480715337
1101	58-59	3.9265377816794427
1101	60-61	4.246662414075914
1101	62-63	3.7910743718040543
1101	64-65	3.5741296607421127
1101	66-67	3.031098002635474
1101	68-69	3.2023759175581503
1101	70-71	3.0898426424545526
1101	72-73	2.793789361311614
1101	74-75	2.245035305786395
1101	76-77	2.5069520071270297
1101	78-79	3.0066938386329625
1101	80-81	2.7158178967008233
1101	82-83	2.7843971946013824
1101	84-85	1.9137224106223947
1101	86-87	2.0070201001015064
1101	88-89	2.156292993061939
1101	90-91	1.9893741531222169
1101	92-93	1.733174911142612
1101	94-95	1.8880839989590896
1101	96-97	1.6982619636782594
1101	98-99	1.8088479411510754
1101	100-101	1.6163658764388344
2201	1	7.187792943888528
2201	2	7.9907527708941934
2201	3	6.4096866969492154
2201	4	8.481247436536414
2201	5	4.080096172899019
2201	6	5.830442912846191
2201	7	6.70975851489322
2201	8	7.874047888448665
2201	9	6.591526301654941
2201	10-11	4.270459687717143
2201	12-13	6.695262606627313
2201	14-15	7.250280658581257
2201	16-17	6.318146059589324
2201	18-19	2.2493908130783247
2201	20-21	5.298321861173015
2201	22-23	5.3779163407783415
2201	24-25	5.831926197614557
2201	26-27	6.3991468622546925
2201	28-29	5.491793047614067
2201	30-31	5.442682961340317
2201	32-33	5.529889985373806
2201	34-35	5.291826761077598
2201	36-37	5.145467992146038
2201	38-39	4.6764309094248375
2201	40-41	4.389481032790439
2201	42-43	4.215366267688658
2201	44-45	4.0425225410320245
2201	46-47	4.511538531412619
2201	48-49	4.332562071289068
2201	50-51	3.952779188063378
2201	52-53	4.703617992242048
2201	54-55	4.316464295546986
2201	56-57	4.589536323984312
2201	58-59	4.181511098003789
2201	60-61	4.085175358158317
2201	62-63	3.868714143830857
2201	64-65	3.765433540395822
2201	66-67	3.298472608039013
2201	68-69	3.6425725098269695
2201	70-71	3.376774288953264
2201	72-73	2.694114817549174
2201	74-75	2.5323625101700564
2201	76-77	2.904614589658536
2201	78-79	3.292590991385328
2201	80-81	2.794027381959147
2201	82-83	3.064241212942086
2201	84-85	2.6635919314589565
2201	86-87	2.515710836465473
2201	88-89	2.476611373208449
2201	90-91	2.337733741610162
2201	92-93	2.255166830774037
2201	94-95	2.3039922383199567
2201	96-97	2.197468100978597
2201	98-99	1.9914088927754428
2201	100-101	1.7079380761911729
2304	1	1.5392000823000451
2304	2	4.87510374441576
2304	3	5.802041199342607
2304	4	4.279946802269439
2304	5	6.4916475279169
2304	6	6.37184513425586
2304	7	7.014483022988355
2304	8	6.62580463913072
2304	9	5.834247308375151
2304	10-11	6.646768667602231
2304	12-13	6.564408798530646
2304	14-15	6.628067674081631
2304	16-17	5.28844701316018
2304	18-19	5.150240467289354
2304	20-21	4.890117635826336
2304	22-23	4.590806918409427
2304	24-25	4.715989380547576
2304	26-27	5.641732624390752
2304	28-29	5.018771136755618
2304	30-31	5.095688843668725
2304	32-33	4.959318882483956
2304	34-35	4.714746925682075
2304	36-37	4.8249552429302796
2304	38-39	4.448385909666598
2304	40-41	3.7833233028480002
2304	42-43	3.2583121774243686
2304	44-45	3.0896617280228433
2304	46-47	4.092765764404202
2304	48-49	3.9264367197187973
2304	50-51	3.499488697371927
2304	52-53	4.1722135201029715
2304	54-55	3.604165055743586
2304	56-57	4.5157363435411
2304	58-59	3.6779015892050104
2304	60-61	3.841711935137198
2304	62-63	3.528912242003134
2304	64-65	3.3745939163742236
2304	66-67	3.0423484073221516
2304	68-69	3.419879673526367
2304	70-71	3.5070559143660844
2304	72-73	3.1695881233886993
2304	74-75	2.429923349764728
2304	76-77	2.5176241454492043
2304	78-79	3.0272137748532693
2304	80-81	2.5992530905720237
2304	82-83	3.034110248914672
2304	84-85	2.4464926288761184
2304	86-87	2.2278073252503674
2304	88-89	2.593655017740268
2304	90-91	2.608802275779773
2304	92-93	2.4243813632873454
2304	94-95	2.6507032769504733
2304	96-97	2.5521020315044396
2304	98-99	2.5057660592785957
2304	100-101	1.9611776512744683
1102	1	6.845454298220858
1102	2	7.289824580319397
1102	3	6.06053614405943
1102	4	5.951989117215813
1102	5	6.420107814138294
1102	6	5.5665914476540586
1102	7	6.738541223411428
1102	8	6.388046596113604
1102	9	4.827259324628912
1102	10-11	5.829168228581885
1102	12-13	5.6588962372363945
1102	14-15	5.9602625169822865
1102	16-17	5.14110845241801
1102	18-19	5.039091466333392
1102	20-21	5.518303082663131
1102	22-23	5.034626749822976
1102	24-25	1.7332474907067308
1102	26-27	5.500599447415272
1102	28-29	4.953735341275319
1102	30-31	4.755360693677751
1102	32-33	5.016248210318302
1102	34-35	4.79142817366586
1102	36-37	4.824862998626365
1102	38-39	3.7460990152891043
1102	40-41	3.7499721228765814
1102	42-43	3.928436384119017
1102	44-45	-3.944555697186747
1102	46-47	1.2311730440258728
1102	48-49	-0.8176271161189987
1102	50-51	2.6411143651225366
1102	52-53	-0.6718734549839454
1102	54-55	2.2602689968337017
1102	56-57	-1.0138964876485232
1102	58-59	2.8579437088922575
1102	60-61	3.8697443884552385
1102	62-63	3.4562978212825968
1102	64-65	3.1026377308551183
1102	66-67	3.0230569269608907
1102	68-69	3.105919323289857
1102	70-71	2.9926311461972155
1102	72-73	2.437855579059754
1102	74-75	2.365139271791712
1102	76-77	2.600927179452956
1102	78-79	-1.6208196582347831
1102	80-81	1.2906432573987168
1102	82-83	2.4243026990224052
1102	84-85	2.1489557602523313
1102	86-87	2.227499670519233
1102	88-89	2.257703781796881
1102	90-91	2.001692915206519
1102	92-93	2.001320669488578
1102	94-95	2.147287631934354
1102	96-97	2.293004806181827
1102	98-99	2.2868555600527714
1102	100-101	1.9220053519436142
2202	1	-20.658684448330188
2202	2	-7.76944479631398
2202	3	-0.7168245517185632
2202	4	1.499960267139823
2202	5	0.6978046769481843
2202	6	1.5931963399718647
2202	7	3.8822913593473025
2202	8	2.3687627807055556
2202	9	2.875311749896614
2202	10-11	1.8403320457146464
2202	12-13	1.1043761261677183
2202	14-15	2.664720088009499
2202	16-17	1.5998687515875858
2202	18-19	1.626611560994359
2202	20-21	1.1390404675278312
2202	22-23	1.0525252594315866
2202	24-25	2.2509339403516933
2202	26-27	3.3978328805048683
2202	28-29	-1.2885578101131756
2202	30-31	0.784502292497308
2202	32-33	1.4011304621871616
2202	34-35	1.3245494025185245
2202	36-37	2.8430421561940733
2202	38-39	2.23053574090774
2202	40-41	1.8155196382359016
2202	42-43	0.44059563342483443
2202	44-45	0.5388377086737037
2202	46-47	-1.6715026891025317
2202	48-49	0.5630001997990277
2202	50-51	1.4790868162151938
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2202	54-55	-0.8456627295881489
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2202	58-59	1.741136349390807
2202	60-61	1.7883379739609069
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2202	64-65	-0.3762595840440994
2202	66-67	0.17411182750161913
2202	68-69	1.2876482253744364
2202	70-71	-2.733692640272235
2202	72-73	-5.020251083190825
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2202	80-81	1.454174018590006
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2202	84-85	-0.2850894891275182
2202	86-87	0.5209280820136257
2202	88-89	1.129429825889325
2202	90-91	1.2728197348870545
2202	92-93	1.1030811201431874
2202	94-95	1.5226945333175976
2202	96-97	1.146478144244142
2202	98-99	1.124362299610322
2202	100-101	0.99867108629687
2305	1	5.915439540888141
2305	2	7.167349874430169
2305	3	6.7598373399098115
2305	4	7.715904198274899
2305	5	7.850606337165505
2305	6	6.1095362774701805
2305	7	2.893364838401933
2305	8	5.95301002505618
2305	9	6.591398758226408
2305	10-11	4.8850371457860575
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2305	96-97	3.003933466324387
2305	98-99	2.6946651709826206
2305	100-101	2.455496564300816
1103	1	-20.658684448330188
1103	2	-7.8029793818113955
1103	3	-0.7071870495838262
1103	4	2.782056005537786
1103	5	4.645724465163887
1103	6	4.519161597735284
1103	7	3.9129767905226984
1103	8	4.210214788370813
1103	9	4.396562219999023
1103	10-11	5.123839242136668
1103	12-13	4.683347576624104
1103	14-15	4.281435640723341
1103	16-17	3.002394139419195
1103	18-19	3.227556299967265
1103	20-21	2.579379466026726
1103	22-23	2.7694692922726993
1103	24-25	3.0778017256894685
1103	26-27	4.082183913625396
1103	28-29	3.6346686551908505
1103	30-31	3.510179297504216
1103	32-33	2.996392198175805
1103	34-35	2.4940357629891388
1103	36-37	2.9206232082936197
1103	38-39	2.4564332394795976
1103	40-41	2.6986797989614395
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1103	54-55	2.539687291287386
1103	56-57	3.5045540769015098
1103	58-59	3.0930737697265727
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1103	62-63	2.9113473957122764
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1103	66-67	2.5249841810609794
1103	68-69	2.916078937228015
1103	70-71	2.8576103386004306
1103	72-73	2.3410157697215546
1103	74-75	2.2262205658343674
1103	76-77	2.451953202115927
1103	78-79	2.9003482736814625
1103	80-81	2.611816099994023
1103	82-83	2.6014138364987396
1103	84-85	2.048030715770384
1103	86-87	1.9728970803422037
1103	88-89	2.025550773284351
1103	90-91	-2.375367016257645
1103	92-93	-2.9745288170736863
1103	94-95	-2.943024816580831
1103	96-97	0.332608776784479
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2203	1	2.4012425970059255
2203	2	3.3266344580169154
2203	3	-6.123671403928114
2203	4	-2.942899311527068
2203	5	-0.00783975003081494
2203	6	0.4142626927986157
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2203	9	-6.174881785276945
2203	10-11	-12.073216652355852
2203	12-13	-9.946738800885182
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2311	1	8.228701415721215
2311	2	8.45723490214548
2311	3	7.543039730813149
2311	4	9.069240472837429
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2205	1	8.431887942242202
2205	2	8.605630252333189
2205	3	7.589144789971979
2205	4	9.281510848787207
2205	5	9.08356939088328
2205	6	7.666548447689895
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2205	86-87	1.3736461305206404
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2205	98-99	0.9313546171060274
2205	100-101	0.46446177798301136
2206	1	8.144874757693085
2206	2	8.31316349025657
2206	3	7.307752496054018
2206	4	8.861492734723925
2206	5	8.857893743236378
2206	6	7.814703509602655
2206	7	8.159507365305377
2206	8	8.6707678654749
2206	9	7.463796277925464
2206	10-11	7.778823663531348
2206	12-13	7.591495074917834
2206	14-15	8.311011068805453
2206	16-17	7.156168790084472
2206	18-19	6.45608775156521
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2206	22-23	6.018160660133809
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2206	28-29	5.903172581819575
2206	30-31	5.673235691957821
2206	32-33	5.918710320033604
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2206	40-41	4.650847719825446
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2206	48-49	4.359575136537579
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2206	52-53	4.841284780542992
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2206	62-63	3.586470984679245
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2206	66-67	3.101458729496237
2206	68-69	3.3708692485169607
2206	70-71	3.1681581919164614
2206	72-73	2.6045248761999282
2206	74-75	2.3135867123679645
2206	76-77	2.549871644626432
2206	78-79	2.8891774226141003
2206	80-81	2.3478485694983284
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2206	84-85	1.9501745193325242
2206	86-87	1.868872176054694
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2206	90-91	2.0746303474380987
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2206	94-95	1.8843214209736132
2206	96-97	1.6970072942050791
2206	98-99	1.4743516649285375
2206	100-101	1.11141246210002
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2104	6	-24.560724279582832
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2104	8	-23.77789743226637
2104	9	-24.687127746715195
2104	10-11	-12.636787239245876
2104	12-13	-4.818576040492093
2104	14-15	-12.795693097960319
2104	16-17	-10.710647718415814
2104	18-19	-3.949614298387356
2104	20-21	-1.0038923629655017
2104	22-23	-2.2738591390728473
2104	24-25	-1.6992436608423631
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2104	28-29	-1.981993605194667
2104	30-31	-3.6272434319299265
2104	32-33	-1.7513225024632888
2104	34-35	-3.881658914774011
2104	36-37	-4.234226822091365
2104	38-39	-2.562499999853074
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2104	42-43	-3.792028914785387
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2104	78-79	-0.6199954346080361
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2104	96-97	-3.1877083102230515
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2110	1	0.6597558441149793
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2110	3	-21.385265984438796
2110	4	-23.35821979094343
2110	5	-11.863217732995642
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2110	7	-24.157398863305165
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2110	14-15	-25.0257445780118
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2110	24-25	-20.085454085030044
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2110	32-33	-19.76509529812073
2110	34-35	-19.894946863277234
2110	36-37	-25.896067233931774
2110	38-39	-15.358100985543416
2110	40-41	-18.177216612995792
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2110	46-47	-4.3643391272606955
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2110	56-57	-2.7373242810823086
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2110	66-67	-3.2703105759937543
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2110	78-79	-2.403636247290276
2110	80-81	-2.223439944038855
2110	82-83	-2.6106396185283742
2110	84-85	-2.526186496652546
2110	86-87	-2.4327641679692675
2110	88-89	-2.4741305453842344
2110	90-91	-2.257595529700012
2110	92-93	-2.105012984792001
2110	94-95	-2.0917255817636793
2110	96-97	-2.1734511901576425
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2109	1	7.786204440558702
2109	2	7.6972666159695535
2109	3	7.016956237783425
2109	4	8.7671135423899
2109	5	8.136296663610548
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2109	7	7.945712247805947
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2109	12-13	6.3762279063118505
2109	14-15	7.325810977543757
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2109	18-19	4.983059232286177
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2109	30-31	4.46564545695896
2109	32-33	4.919373336232553
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2109	36-37	5.055043877179337
2109	38-39	4.351748820395748
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2109	56-57	3.8247154336933917
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2109	60-61	3.1430274436823247
2109	62-63	2.4222816166664707
2109	64-65	2.4920202911844704
2109	66-67	2.3215563795076477
2109	68-69	2.2063271540611105
2109	70-71	1.9349544501870533
2109	72-73	1.6266489921022789
2109	74-75	1.3167845220873353
2109	76-77	1.4936548981057811
2109	78-79	2.306217349604747
2109	80-81	1.7733922610338055
2109	82-83	1.882898065378118
2109	84-85	1.4129589135830365
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2108	1	6.707502336461246
2108	2	7.318066911281473
2108	3	6.113442025897122
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2108	7	6.605613318311665
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2108	64-65	2.8183562084971285
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2108	76-77	1.8763806271570935
2108	78-79	2.1727998523732985
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2108	84-85	1.6853723502755393
2108	86-87	1.646426537021977
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2108	92-93	1.4137531763239224
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2211	1	6.617217763544083
2211	2	6.169900427895563
2211	3	5.55303436480451
2211	4	7.602587349141942
2211	5	7.232638663351853
2211	6	6.8323684639173585
2211	7	6.8263031157402345
2211	8	6.597345097807356
2211	9	5.387377491934398
2211	10-11	5.731166653446088
2211	12-13	5.63252877295265
2211	14-15	6.398197990866745
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2211	32-33	4.930925270005673
2211	34-35	4.451028295969078
2211	36-37	4.562993689622743
2211	38-39	4.330184469082852
2211	40-41	3.5888004592300184
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2211	44-45	3.631719542574835
2211	46-47	3.9536981436419687
2211	48-49	3.527855549510015
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2211	54-55	3.128955242111008
2211	56-57	3.5710636422045816
2211	58-59	3.247712882209047
2211	60-61	0.39458558622533246
2211	62-63	0.938701743428858
2211	64-65	2.4382751094486004
2211	66-67	2.523229384293572
2211	68-69	2.8244381358228487
2211	70-71	2.5208584729525434
2211	72-73	2.110733845290742
2211	74-75	1.733197405284855
2211	76-77	2.062835599179383
2211	78-79	2.315911826386536
2211	80-81	2.4342827021151656
2211	82-83	2.725071652236224
2211	84-85	1.9004653163769802
2211	86-87	2.0084350362641423
2211	88-89	2.0300542903509964
2211	90-91	1.8626279872768556
2211	92-93	1.8436269081306769
2211	94-95	1.9264650800522283
2211	96-97	1.6908270103960916
2211	98-99	1.8485547519429488
2211	100-101	1.4251252618711767
2210	1	7.37497802922816
2210	2	7.630407649282937
2210	3	6.831520371036966
2210	4	8.406591699182243
2210	5	8.318395207396708
2210	6	7.458126707849846
2210	7	7.74969269863379
2210	8	7.4963395515756375
2210	9	7.0431235997839075
2210	10-11	7.068805439865649
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2210	14-15	4.9533846859020265
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2210	24-25	5.883233885952954
2210	26-27	4.715434608751021
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2210	30-31	5.24895345616104
2210	32-33	5.485363561646473
2210	34-35	5.097835711937851
2210	36-37	5.250252335188513
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2210	40-41	4.584216597294333
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2210	44-45	4.428587935045446
2210	46-47	4.486764615518233
2210	48-49	4.500304568556423
2210	50-51	4.595947644084031
2210	52-53	4.712227311482053
2210	54-55	4.450268140565711
2210	56-57	4.593120380830836
2210	58-59	4.2416234179592855
2210	60-61	4.127768716373325
2210	62-63	3.835709105196294
2210	64-65	3.616493261461745
2210	66-67	3.3323048335271963
2210	68-69	3.6342631205482405
2210	70-71	3.5943454336300995
2210	72-73	3.234110591942695
2210	74-75	2.6931507698431716
2210	76-77	2.880808897706821
2210	78-79	3.6374868489065655
2210	80-81	3.481418792053155
2210	82-83	3.1881468186196926
2210	84-85	2.4174723785740575
2210	86-87	2.4408539659062143
2210	88-89	2.8475791912663553
2210	90-91	2.741290409101186
2210	92-93	2.4908228947238555
2210	94-95	2.845368335546034
2210	96-97	2.720433705863311
2210	98-99	2.6274005038610717
2210	100-101	2.3367622815222937
2209	1	5.131870864306453
2209	2	2.5014679471651995
2209	3	4.53821455775622
2209	4	1.9168130031099153
2209	5	4.917779440659572
2209	6	5.33302298757939
2209	7	6.455194053179316
2209	8	7.180563433496637
2209	9	6.315495777552403
2209	10-11	6.327883956404769
2209	12-13	5.624814895574833
2209	14-15	4.9830005028801985
2209	16-17	4.348541116243069
2209	18-19	3.738677169418761
2209	20-21	4.081202085596367
2209	22-23	4.153118028612667
2209	24-25	4.4522486866491064
2209	26-27	1.6548796336713636
2209	28-29	0.45649355771313793
2209	30-31	3.146623351337823
2209	32-33	4.400860194319321
2209	34-35	4.580809691004607
2209	36-37	4.12820036554352
2209	38-39	-0.8342688641011655
2209	40-41	0.1642139714150197
2209	42-43	-4.290972076824307
2209	44-45	0.6503248972995088
2209	46-47	2.6593890975056453
2209	48-49	1.9549766515734603
2209	50-51	3.0419781702254625
2209	52-53	3.969709301247015
2209	54-55	3.122795084285542
2209	56-57	3.5756251164412944
2209	58-59	2.8139035918246798
2209	60-61	2.79689850232295
2209	62-63	2.867002600002234
2209	64-65	2.289024032135739
2209	66-67	2.5473670970901168
2209	68-69	-0.6134810371646573
2209	70-71	1.928513795277663
2209	72-73	2.3281395425565385
2209	74-75	1.9198348063024682
2209	76-77	2.3583659703498654
2209	78-79	2.6531281011958647
2209	80-81	2.2074588208161536
2209	82-83	2.27093149102064
2209	84-85	1.4368872039197207
2209	86-87	1.623186056820515
2209	88-89	1.819391416952243
2209	90-91	1.728819935524438
2209	92-93	1.4822367796200666
2209	94-95	1.9415588283713774
2209	96-97	1.6187376979063863
2209	98-99	1.5488832778572466
2209	100-101	1.3663100964688475
2208	1	4.445742885644787
2208	2	6.219678336641138
2208	3	4.6363894534822805
2208	4	3.079701287016146
2208	5	4.06750402111242
2208	6	4.961893622245849
2208	7	3.56637399521264
2208	8	3.8351920768770356
2208	9	4.098724033843034
2208	10-11	4.514370070958595
2208	12-13	5.039948577897778
2208	14-15	5.706209223720631
2208	16-17	4.032602027961943
2208	18-19	2.0130985863659525
2208	20-21	4.473345737227362
2208	22-23	3.497727241753182
2208	24-25	4.0633163692826315
2208	26-27	5.291146132225986
2208	28-29	4.468693745155825
2208	30-31	4.398823298901
2208	32-33	3.853040109839654
2208	34-35	4.0575327517997515
2208	36-37	3.6782831029305925
2208	38-39	3.9776450881745973
2208	40-41	3.6059487126586767
2208	42-43	3.7644666017678787
2208	44-45	-0.9198881493679956
2208	46-47	2.9504493144156356
2208	48-49	3.1259814899851506
2208	50-51	3.4846340277252565
2208	52-53	2.075025662333843
2208	54-55	0.9790738040696674
2208	56-57	-0.8612135578583135
2208	58-59	2.27791798375328
2208	60-61	3.2599364373728363
2208	62-63	2.796286963688182
2208	64-65	3.0950484165186545
2208	66-67	3.319477885228956
2208	68-69	2.548262348157998
2208	70-71	0.5493383663457543
2208	72-73	0.4216880253607549
2208	74-75	-1.7946957474025567
2208	76-77	0.8184122502606321
2208	78-79	2.170286219244357
2208	80-81	2.5840514418700806
2208	82-83	2.5295908255107236
2208	84-85	1.7198674795118123
2208	86-87	2.369566190925781
2208	88-89	2.5422033897170806
2208	90-91	2.21612357626406
2208	92-93	2.246760536739121
2208	94-95	2.298597903059335
2208	96-97	2.377693094206265
2208	98-99	2.6044631486842427
2208	100-101	2.471357329585011
1307	1	7.218231023740994
1307	2	7.235905820671039
1307	3	6.924669754562686
1307	4	8.344202354385288
1307	5	4.476305451439405
1307	6	5.580155601781481
1307	7	3.9014246661065997
1307	8	5.304158919686174
1307	9	5.734523266631317
1307	10-11	6.651606814719109
1307	12-13	5.85826305762728
1307	14-15	5.545189678043567
1307	16-17	4.4354491941170835
1307	18-19	5.110127722427325
1307	20-21	5.282947492213452
1307	22-23	4.7775385265116626
1307	24-25	5.497118667872904
1307	26-27	6.28471242020299
1307	28-29	5.739578807554224
1307	30-31	5.187800232594711
1307	32-33	5.793804928477293
1307	34-35	5.557619340248191
1307	36-37	5.162509137793386
1307	38-39	4.460610357167319
1307	40-41	3.8571451318933008
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1212	56-57	-3.891333845711536
1212	58-59	-2.8485413169099694
1212	60-61	-14.032581110478532
1212	62-63	-11.78574999653669
1212	64-65	-15.836214948239057
1212	66-67	-12.149148408962834
1212	68-69	-7.762763501447957
1212	70-71	-14.795727093281101
1212	72-73	-11.389315675494187
1212	74-75	-7.91220292561141
1212	76-77	-18.675403214400028
1212	78-79	-9.384132255230213
1212	80-81	-7.192937817999205
1212	82-83	-4.722088452520531
1212	84-85	-4.055543178467172
1212	86-87	-3.2298293495238752
1212	88-89	-3.204012194144127
1212	90-91	-3.4639981210551376
1212	92-93	-3.413748441559129
1212	94-95	-3.0257010889711395
1212	96-97	-13.113962867230745
1212	98-99	-10.289429232548116
1212	100-101	-6.500686919592464
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	232.0
3	533.0
4	243.0
5	1424.0
6	1843.0
7	1555.0
8	3072.0
9	5907.0
10	7773.0
11	9997.0
12	13818.0
13	17793.0
14	20581.0
15	22080.0
16	23577.0
17	25942.0
18	29141.0
19	32022.0
20	34558.0
21	38265.0
22	42663.0
23	45615.0
24	48205.0
25	49592.0
26	51898.0
27	53887.0
28	54590.0
29	55973.0
30	57154.0
31	61054.0
32	65968.0
33	74926.0
34	84008.0
35	91799.0
36	94888.0
37	87117.0
38	32958.0
39	106.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.19680499877016	30.820283338439697	16.317463331276404	23.66544833151374
2	25.105036472684656	29.98978748074157	24.710526602397888	20.19464944417589
3	20.457371804929124	27.04379664510333	28.79944442884676	23.69938712112079
4	23.160868172082903	26.216672007015536	27.40357859588175	23.218881225019818
5	22.323098558056962	27.38994780797599	27.01127888908477	23.27567474488227
6	22.229683347398385	27.446767977025683	26.66615759575781	23.65739107981812
7	23.403400048864558	27.785857672065976	27.126928994512646	21.68381328455682
8	21.632875658705018	27.099360647938642	28.318389701409156	22.94937399194718
9	22.450893251131536	26.573433185823976	28.09422948422154	22.881444078822952
10-11	23.036431528500863	27.062022921644264	26.9992380671973	22.902307482657573
12-13	22.921875485943225	27.187585993497265	26.818830130192435	23.071708390367075
14-15	22.94289211794242	27.35582325119548	26.75465379690732	22.946630833954778
16-17	23.301439324601063	27.240794536566256	26.63427778168917	22.82348835714351
18-19	23.375699671247265	27.210507477192802	26.423746166441692	22.99004668511824
20-21	23.221246271002872	27.336998338910014	26.502686876466715	22.939068513620402
22-23	23.11601878569657	27.14033330577893	26.771386223694353	22.972261684830148
24-25	23.088237450916367	27.158193830456824	26.764945854589723	22.98862286403709
26-27	22.991605593778043	27.218721956326654	26.799157443414103	22.990515006481203
28-29	23.234619053014384	27.16445816026977	26.723186342514087	22.87773644420176
30-31	23.275768114218906	27.069905196112725	26.56170331466827	23.092623375000095
32-33	23.041556634203488	27.179584122435635	26.755937526535543	23.02292171682533
34-35	23.218639965876353	27.151108904462152	26.692895544993718	22.937355584667777
36-37	23.22474172258768	27.183803387713734	26.557623893948225	23.033830995750364
38-39	23.12012513491202	27.166088499264735	26.816997743976444	22.8967886218468
40-41	23.291201342034046	27.143633364256587	26.547826919207413	23.017338374501957
42-43	23.239730434437398	27.16381643355203	26.61783905614601	22.978614075864563
44-45	23.094264321083905	27.042152111681094	26.830791547388767	23.032792019846234
46-47	23.275725671880647	27.252463087205236	26.563127194650253	22.90868404626386
48-49	23.229820365199213	27.060983342392465	26.699661763169342	23.009534529238984
50-51	23.26576042318098	27.123821370836094	26.541463268840005	23.06895493714293
52-53	23.195396601675373	27.12664500502337	26.7387516462938	22.93920674700746
54-55	23.216238534580892	27.082570742219104	26.61669000116451	23.084500722035493
56-57	23.076634190919158	27.158313294420093	26.780675090407456	22.98437742425329
58-59	23.285540995292404	27.192407609341334	26.53053657222465	22.991514823141614
60-61	23.329253227250575	27.0041443299748	26.49738004299223	23.169222399782395
62-63	23.257712843991662	27.06127069552089	26.593128391951986	23.087888068535463
64-65	23.386687791769432	27.052637175228885	26.57200589562304	22.98866913737865
66-67	23.44113146468592	27.019501632438757	26.474231884948086	23.06513501792724
68-69	23.275927448573114	27.109919691832896	26.610612392011983	23.003540467582006
70-71	23.504227043371912	27.02185392520844	26.437633006187173	23.036286025232474
72-73	23.438991903054298	27.011583782853076	26.478458457219904	23.070965856872714
74-75	23.333653917123872	27.00482292218835	26.52458094852543	23.136942212162353
76-77	23.520022325169386	27.115317280758454	26.390871519547993	22.973788874524168
78-79	23.631171778426534	26.99993612648459	26.347825106800276	23.021066988288602
80-81	23.678119274707583	26.9959828406975	26.31342059900682	23.01247728558809
82-83	23.59988938247655	27.086324702846383	26.35215515192883	22.96163076274824
84-85	23.78988670471123	26.90942897758501	26.172609033440835	23.128075284262927
86-87	23.63599834201094	27.08653897765293	26.28814716740322	22.98931551293291
88-89	23.786914681349185	27.002864402623356	26.341025654403822	22.869195261623638
90-91	23.95644066665847	26.925222871109888	25.9836089708254	23.134727491406245
92-93	23.888246866753757	26.962256310323244	26.134507823510145	23.014988999412857
94-95	24.111986872434617	26.916597233887884	26.02395421514646	22.947461678531038
96-97	24.154010797531885	26.92370519142014	25.950746119695385	22.971537891352593
98-99	24.129937915785753	27.000596920605087	25.9333311970382	22.936133966570956
100-101	24.588809282452228	26.875794053055806	25.600942338498378	22.934454325993585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	103.0
2	108.0
3	103.0
4	100.5
5	135.0
6	175.5
7	234.0
8	291.5
9	363.0
10	472.0
11	654.5
12	927.0
13	1204.5
14	1550.5
15	1997.5
16	2528.5
17	3159.0
18	3949.0
19	4814.0
20	5943.0
21	7326.0
22	8829.0
23	10574.5
24	12512.0
25	14691.5
26	16984.0
27	19204.0
28	21600.5
29	23921.0
30	26194.0
31	28488.0
32	30600.0
33	32743.5
34	34554.0
35	36179.0
36	37437.5
37	38286.5
38	38833.5
39	38449.5
40	37517.0
41	36464.5
42	34973.5
43	33300.5
44	31873.0
45	30608.0
46	29582.0
47	28548.5
48	27550.5
49	26553.0
50	25560.5
51	24981.0
52	24745.5
53	24631.5
54	24539.0
55	24843.0
56	25253.0
57	25637.5
58	26150.0
59	26122.0
60	25722.5
61	25207.5
62	24285.5
63	23029.0
64	21589.0
65	19812.5
66	17854.5
67	16121.0
68	14356.0
69	12558.5
70	10950.5
71	9509.5
72	8167.5
73	6839.0
74	5736.0
75	4847.0
76	4066.0
77	3354.5
78	2653.0
79	2097.0
80	1689.5
81	1337.5
82	1082.5
83	910.5
84	717.0
85	585.5
86	472.0
87	349.0
88	269.5
89	226.0
90	186.0
91	133.0
92	102.0
93	82.5
94	59.0
95	43.5
96	26.0
97	10.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	13.708511666667908
2	11.105806933048944
3	9.81197640377224
4	11.678732637402002
5	9.176790737266684
6	5.397030140226415
7	9.774292742469411
8	10.145022517104733
9	5.4875900851755
10-11	6.411025971192107
12-13	5.412408946667193
14-15	7.373970122665531
16-17	2.683508631867121
18-19	3.145431377382505
20-21	3.113296002180588
22-23	3.1501976902745623
24-25	3.519922070784215
26-27	4.397296011117425
28-29	1.7334111831105705
30-31	2.2848512426299026
32-33	3.085926939870729
34-35	2.051004016363348
36-37	3.1585015010161928
38-39	1.3960828355391184
40-41	1.1778750734496264
42-43	0.5749364926043953
44-45	1.3233965639352467
46-47	1.3894174448541323
48-49	1.2814306683934622
50-51	1.5749312794496695
52-53	2.4856694100272794
54-55	1.8326845438154482
56-57	1.8392382240420269
58-59	1.584501142053253
60-61	1.4345112332313292
62-63	0.6285947494595076
64-65	1.2428533234233743
66-67	0.568829654211447
68-69	0.8201037119895856
70-71	1.0155597773833986
72-73	0.08489994838976822
74-75	0.10828467101642368
76-77	0.4579384058321796
78-79	0.8938326145385947
80-81	0.30716652380140264
82-83	0.4931272002305704
84-85	0.0677337746144686
86-87	0.10225230626241383
88-89	0.08311258105524677
90-91	0.0916398127136928
92-93	0.1768004188397454
94-95	0.4716043185773748
96-97	0.3825338464070565
98-99	0.06479206587640206
100-101	0.23168749073734116
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1342757.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.93263592576145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.0374508155901	97.9803606455428
2	0.8806822936093293	1.7425644143983263
3	0.05507454748625627	0.16346010465701555
4	0.019121113833528748	0.07566808773550161
5	0.007671229480797456	0.037946747666335136
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.978945557535727E-4	0.0	0.0
2	4.4684183363035904E-4	0.0	0.0
3	6.702627504455385E-4	0.0	0.0
4	8.936836672607181E-4	0.0	0.0
5	0.0015639464177062566	0.0	0.0
6	0.0017873673345214362	0.0	0.0
7	0.002159735529213402	0.0	0.0
8	0.002681051001782154	0.0	0.0
9	0.00305341919647412	0.0	0.0
10-11	0.0033513137522276928	0.0	0.0
12-13	0.0037236819469196585	0.0	0.0
14-15	0.0042077606000192145	0.0	0.0
16-17	0.004803549711526359	0.0	0.0
18-19	0.005659996559317881	0.0	0.0
20-21	0.00610683839294824	0.0	0.0
22-23	0.006367496129232616	0.0	0.0
24-25	0.006926048421270565	7.447363893839318E-5	0.0
26-27	0.007596311171716103	7.447363893839318E-5	0.0
28-29	0.008564468477915215	7.447363893839318E-5	0.0
30-31	0.009867757159337095	7.447363893839318E-5	0.0
32-33	0.01232538724430407	7.447363893839318E-5	0.0
34-35	0.01537880644077819	7.447363893839318E-5	3.723681946919659E-5
36-37	0.019251435665574634	7.447363893839318E-5	7.447363893839318E-5
38-39	0.02431564311338537	7.447363893839318E-5	7.447363893839318E-5
40-41	0.030683139242617986	7.447363893839318E-5	7.447363893839318E-5
42-43	0.03857734497008766	7.447363893839318E-5	1.1171045840758977E-4
44-45	0.04930154897721628	7.447363893839318E-5	1.4894727787678635E-4
46-47	0.06214825169408911	7.447363893839318E-5	1.4894727787678635E-4
48-49	0.07708021630123693	7.447363893839318E-5	1.4894727787678635E-4
50-51	0.09428362689600575	7.447363893839318E-5	1.4894727787678635E-4
52-53	0.11602992946601656	7.447363893839318E-5	1.4894727787678635E-4
54-55	0.13870715252275728	7.447363893839318E-5	1.4894727787678635E-4
56-57	0.16845937127864535	7.447363893839318E-5	1.4894727787678635E-4
58-59	0.2042439547885433	7.447363893839318E-5	1.4894727787678635E-4
60-61	0.2455768243993515	7.447363893839318E-5	1.4894727787678635E-4
62-63	0.2903354814013258	7.447363893839318E-5	1.4894727787678635E-4
64-65	0.3406051876847412	7.447363893839318E-5	2.2342091681517952E-4
66-67	0.39646041688853606	7.447363893839318E-5	2.2342091681517952E-4
68-69	0.4586831422215636	1.1171045840758977E-4	2.978945557535727E-4
70-71	0.5324120447705728	1.8618409734598294E-4	2.978945557535727E-4
72-73	0.6101625238222552	2.2342091681517952E-4	2.978945557535727E-4
74-75	0.6955093140456539	2.2342091681517952E-4	2.978945557535727E-4
76-77	0.7874470213151001	2.2342091681517952E-4	2.978945557535727E-4
78-79	0.8896620907580448	2.2342091681517952E-4	2.978945557535727E-4
80-81	1.0136606995904696	2.2342091681517952E-4	2.978945557535727E-4
82-83	1.1434310154406195	2.2342091681517952E-4	2.978945557535727E-4
84-85	1.2824360625191304	2.2342091681517952E-4	2.978945557535727E-4
86-87	1.4354793905375285	2.2342091681517952E-4	3.351313752227693E-4
88-89	1.6055399450533492	2.978945557535727E-4	3.723681946919659E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAACGT	150	0.008665061	17.843128	4
ACTGCAG	880	0.0029373202	6.6911736	4
CTGCAGC	1270	0.0048588184	5.3552976	5
GTGTAGG	1255	3.1510353E-8	5.059569	94-95
>>END_MODULE