##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib15_ATGTCA_L007_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1342757
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24204602917728	33.0	33.0	33.0	27.0	33.0
2	31.66925735631987	33.0	33.0	33.0	27.0	33.0
3	31.815160151836857	33.0	33.0	33.0	27.0	33.0
4	35.63649938149643	37.0	37.0	37.0	33.0	37.0
5	35.14688212386903	37.0	37.0	37.0	33.0	37.0
6	34.94210642729846	37.0	33.0	37.0	33.0	37.0
7	34.74383302414361	37.0	33.0	37.0	27.0	37.0
8	34.791452213617205	37.0	33.0	37.0	27.0	37.0
9	34.69263835526458	37.0	33.0	37.0	27.0	37.0
10-11	34.539726473218906	37.0	33.0	37.0	27.0	37.0
12-13	34.55100997425447	37.0	33.0	37.0	27.0	37.0
14-15	35.47102379656185	37.0	33.0	40.0	27.0	40.0
16-17	35.37755602838041	37.0	33.0	40.0	27.0	40.0
18-19	35.5956658576347	37.0	33.0	40.0	27.0	40.0
20-21	35.26890792600597	37.0	33.0	40.0	27.0	40.0
22-23	35.296601693381604	37.0	33.0	40.0	27.0	40.0
24-25	35.129784465841546	37.0	33.0	40.0	27.0	40.0
26-27	34.75056581347184	37.0	33.0	40.0	27.0	40.0
28-29	34.3868730529798	37.0	33.0	40.0	27.0	40.0
30-31	34.27968872997869	37.0	33.0	40.0	24.5	40.0
32-33	34.2717967584604	37.0	33.0	40.0	24.5	40.0
34-35	34.11691728287397	37.0	33.0	38.5	24.5	40.0
36-37	33.80672638459528	37.0	33.0	37.0	22.0	40.0
38-39	33.70725455164263	37.0	33.0	37.0	22.0	40.0
40-41	33.4088725659222	37.0	33.0	37.0	22.0	40.0
42-43	33.01990829316101	37.0	33.0	37.0	22.0	40.0
44-45	32.88049997132765	37.0	33.0	37.0	22.0	40.0
46-47	32.72235482667378	37.0	33.0	37.0	22.0	40.0
48-49	32.73231902719554	37.0	33.0	37.0	22.0	40.0
50-51	33.0289099218995	37.0	33.0	37.0	22.0	40.0
52-53	32.869753425228836	37.0	33.0	37.0	22.0	40.0
54-55	32.498591703487676	37.0	33.0	37.0	22.0	40.0
56-57	32.536179293796266	37.0	33.0	37.0	22.0	40.0
58-59	32.23634618922114	37.0	30.0	37.0	22.0	40.0
60-61	32.137028889069285	33.0	27.0	37.0	22.0	40.0
62-63	32.10682424295685	33.0	27.0	37.0	22.0	40.0
64-65	31.772753372352554	33.0	27.0	37.0	22.0	40.0
66-67	31.425761325392457	33.0	27.0	37.0	18.5	40.0
68-69	31.251032390819784	33.0	27.0	37.0	15.0	40.0
70-71	31.09721230274726	33.0	27.0	37.0	15.0	38.5
72-73	30.876583402655882	33.0	27.0	37.0	15.0	37.0
74-75	30.53273414325898	33.0	27.0	37.0	15.0	37.0
76-77	29.461256206446883	33.0	27.0	35.0	15.0	37.0
78-79	30.393839317166098	33.0	27.0	37.0	15.0	37.0
80-81	30.11182998859809	33.0	27.0	37.0	15.0	37.0
82-83	30.090539464698377	33.0	27.0	37.0	15.0	37.0
84-85	29.974406389242432	33.0	27.0	37.0	15.0	37.0
86-87	29.779124592163736	33.0	27.0	37.0	15.0	37.0
88-89	29.64594226654562	33.0	27.0	37.0	15.0	37.0
90-91	29.559562154581954	33.0	27.0	37.0	15.0	37.0
92-93	29.353281345768444	33.0	27.0	33.0	15.0	37.0
94-95	29.259447912019823	33.0	27.0	33.0	15.0	37.0
96-97	29.120174759841134	33.0	27.0	33.0	15.0	37.0
98-99	28.850920903782292	33.0	27.0	33.0	15.0	37.0
100-101	28.03054275643322	33.0	24.5	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	1.1249560770538025
2306	2	0.5131158949075179
2306	3	0.5370158100931732
2306	4	0.4837569604323875
2306	5	0.6602324665517898
2306	6	0.7466260134421674
2306	7	0.8811417596697453
2306	8	0.6686583129606376
2306	9	1.0473003788977948
2306	10-11	1.2496937530776648
2306	12-13	1.4326991977945909
2306	14-15	1.534882576548675
2306	16-17	1.480704419902203
2306	18-19	1.4296024079849587
2306	20-21	1.6595439880704532
2306	22-23	1.3470646634455932
2306	24-25	1.3502479948576536
2306	26-27	1.5023500777128689
2306	28-29	1.7431468771433174
2306	30-31	1.4893690713873795
2306	32-33	1.156456921384141
2306	34-35	1.173209682128551
2306	36-37	1.5876640368553225
2306	38-39	1.1037809520515296
2306	40-41	1.2635399663120523
2306	42-43	1.309544486286093
2306	44-45	1.2198391067171954
2306	46-47	1.0130182234008203
2306	48-49	1.020754886183255
2306	50-51	1.0866701819194091
2306	52-53	1.1996907352066515
2306	54-55	1.7767224667433226
2306	56-57	1.448732851624058
2306	58-59	1.0696336623344145
2306	60-61	0.9905855815156812
2306	62-63	0.8330363074912981
2306	64-65	0.8253418717420757
2306	66-67	0.9758705349457983
2306	68-69	0.8794587219768744
2306	70-71	0.7496676884557516
2306	72-73	0.7696458467263092
2306	74-75	1.0334304800679313
2306	76-77	0.8987727432086174
2306	78-79	0.9840207671287544
2306	80-81	1.4972055711435566
2306	82-83	1.315198672462376
2306	84-85	1.150212641100147
2306	86-87	0.8710915324379762
2306	88-89	0.5902584040668728
2306	90-91	0.42845498076231436
2306	92-93	0.22991574145395788
2306	94-95	0.2547821521777962
2306	96-97	0.26152719115665946
2306	98-99	0.6139223724602232
2306	100-101	0.38066449523668666
2307	1	0.6139361147054565
2307	2	0.42858406550521977
2307	3	0.44650771055979277
2307	4	0.38076939056558246
2307	5	0.6879979721903524
2307	6	0.7097619856569963
2307	7	0.9002136845058502
2307	8	0.8442217572252133
2307	9	1.024069651114928
2307	10-11	1.272734668285139
2307	12-13	1.417252610723608
2307	14-15	1.2869994224382921
2307	16-17	1.4646072066616753
2307	18-19	1.3543227052606568
2307	20-21	1.4371027840464308
2307	22-23	1.1774263859264167
2307	24-25	1.3697105544449073
2307	26-27	1.5396211074625725
2307	28-29	1.546617856778738
2307	30-31	1.6746960883108457
2307	32-33	1.3865687553739718
2307	34-35	1.5853287558431859
2307	36-37	1.4986577763373319
2307	38-39	1.1648022802968185
2307	40-41	1.2855056497998163
2307	42-43	1.407180978248256
2307	44-45	1.0797911025045792
2307	46-47	1.1246138877613276
2307	48-49	1.1496486933478067
2307	50-51	1.1566734600720778
2307	52-53	1.4261652798330928
2307	54-55	1.8009885679600544
2307	56-57	1.4948906306191105
2307	58-59	1.3669000550994959
2307	60-61	1.1426747326672526
2307	62-63	0.9172377311327153
2307	64-65	1.0161485492825406
2307	66-67	1.174260118898303
2307	68-69	1.1219407545445748
2307	70-71	0.9498155703499442
2307	72-73	0.8535753273510593
2307	74-75	0.9736512937939672
2307	76-77	1.053853267543488
2307	78-79	1.1049977885031552
2307	80-81	1.7882116814128892
2307	82-83	1.5539569284851567
2307	84-85	1.2964901951272623
2307	86-87	1.2689803738606393
2307	88-89	1.1082026985504854
2307	90-91	0.9907481239125531
2307	92-93	0.7066585788552331
2307	94-95	0.7815945325735001
2307	96-97	0.7848186189287318
2307	98-99	0.8595955995142148
2307	100-101	1.0803497972906655
1101	1	0.9659013423143108
1101	2	0.5649550366398053
1101	3	0.3612593655205245
1101	4	0.38281367003224176
1101	5	0.6828956387996783
1101	6	0.48473254655360165
1101	7	0.7552986144854543
1101	8	0.7627125693031687
1101	9	0.8126352402734369
1101	10-11	0.8944869559759496
1101	12-13	1.139962383051099
1101	14-15	1.0127593683573934
1101	16-17	1.2265277552103981
1101	18-19	0.974621859652494
1101	20-21	1.2110215630361978
1101	22-23	0.8722457826493866
1101	24-25	1.0862530573815405
1101	26-27	0.985231538016059
1101	28-29	1.2492281744616065
1101	30-31	1.3451927177714325
1101	32-33	0.9670365876751603
1101	34-35	1.076127679704996
1101	36-37	1.1762072426993981
1101	38-39	0.8081814735277746
1101	40-41	0.9603591332037738
1101	42-43	1.172114177882662
1101	44-45	0.9090455757752807
1101	46-47	0.6645321965983442
1101	48-49	0.6893855726852465
1101	50-51	0.7586313832189475
1101	52-53	1.0083242920903999
1101	54-55	1.3515060451431538
1101	56-57	1.1065782789739416
1101	58-59	1.0029193209362113
1101	60-61	0.6081842149500218
1101	62-63	0.5638651973888216
1101	64-65	0.41812867071967474
1101	66-67	0.6645593690664882
1101	68-69	0.4179001565267484
1101	70-71	0.6256863440518892
1101	72-73	0.4510989268250434
1101	74-75	0.5699427729844935
1101	76-77	0.5760500423537707
1101	78-79	0.7111516249581342
1101	80-81	1.214772378218555
1101	82-83	0.9369121089126438
1101	84-85	0.8376969989335308
1101	86-87	0.6646521679565218
1101	88-89	0.4765416202518651
1101	90-91	0.5698766634093282
1101	92-93	0.6185863470008215
1101	94-95	0.576145840387408
1101	96-97	0.5477982235372991
1101	98-99	0.6831947825298421
1101	100-101	0.8469041631584986
2201	1	1.5003439957605522
2201	2	0.887823666372352
2201	3	0.36326901933046685
2201	4	0.25377719410138866
2201	5	0.43651252121020434
2201	6	0.5465670341468183
2201	7	0.7475632602908391
2201	8	0.6615618254895281
2201	9	0.6787202170948419
2201	10-11	0.891209319390363
2201	12-13	1.0663930115376417
2201	14-15	1.241457202787842
2201	16-17	1.080533932506036
2201	18-19	0.9746482301781612
2201	20-21	1.2661277208835742
2201	22-23	1.0141823901032367
2201	24-25	1.0170765759348583
2201	26-27	1.209784255443786
2201	28-29	1.426548533496316
2201	30-31	1.448077873033391
2201	32-33	1.1172843058006166
2201	34-35	1.1262811232011956
2201	36-37	1.1297286418315977
2201	38-39	0.8258315959859104
2201	40-41	1.3116337635494375
2201	42-43	1.1976584270753392
2201	44-45	0.9861689250078385
2201	46-47	1.11674660655234
2201	48-49	1.2093563014017619
2201	50-51	1.1306348948939515
2201	52-53	1.2524131739471542
2201	54-55	1.6488177898061451
2201	56-57	1.332974186268359
2201	58-59	1.7060564245954417
2201	60-61	1.2275948996277606
2201	62-63	1.0185957949998752
2201	64-65	0.9579113556127723
2201	66-67	1.0997741295287113
2201	68-69	1.1056401695142313
2201	70-71	1.3326581616513558
2201	72-73	1.0934640553209185
2201	74-75	1.2277797086415099
2201	76-77	1.0760297150735738
2201	78-79	0.9737943691629383
2201	80-81	1.4995883394187217
2201	82-83	1.1027277767407107
2201	84-85	1.2046736291539375
2201	86-87	1.2348889642892615
2201	88-89	1.294150978272409
2201	90-91	1.093720563082659
2201	92-93	1.0098293546911243
2201	94-95	0.8215763227372861
2201	96-97	0.8164149905767779
2201	98-99	1.1112719207124968
2201	100-101	1.2406287471909252
2304	1	-1.9208215422201569
2304	2	-0.2667180259567985
2304	3	0.02566347101215527
2304	4	0.09362798788900051
2304	5	0.497192618413294
2304	6	0.44622080492184324
2304	7	0.6845009737876637
2304	8	0.810334884459408
2304	9	1.026487260268219
2304	10-11	0.9848752694383833
2304	12-13	1.1738530595459906
2304	14-15	1.2386763856757526
2304	16-17	1.270299694447452
2304	18-19	1.0532735303848284
2304	20-21	1.311534680410766
2304	22-23	1.1501453693892714
2304	24-25	1.2370286818961702
2304	26-27	1.1898894758847334
2304	28-29	1.6163203972779172
2304	30-31	1.6490189621608877
2304	32-33	1.3244225473923947
2304	34-35	1.140983468485942
2304	36-37	1.2279854156079537
2304	38-39	1.2175931385434637
2304	40-41	0.9288789232840315
2304	42-43	0.9738232336076109
2304	44-45	1.0951974548953487
2304	46-47	1.0200966249428234
2304	48-49	0.9784469897310473
2304	50-51	0.910662706926999
2304	52-53	1.363500804559358
2304	54-55	1.4943664665023846
2304	56-57	1.2414230258914571
2304	58-59	0.884271712037684
2304	60-61	0.5498023384042838
2304	62-63	0.3597990346290274
2304	64-65	0.4055925559358755
2304	66-67	0.6176459559732272
2304	68-69	0.6489270099149671
2304	70-71	0.36821458845700406
2304	72-73	0.33883611850137996
2304	74-75	0.5828433168496154
2304	76-77	0.5869233535112741
2304	78-79	0.5912625163986398
2304	80-81	1.3142318135315492
2304	82-83	0.907075283799152
2304	84-85	0.39588622236340854
2304	86-87	0.4706391206585714
2304	88-89	0.3465523458878863
2304	90-91	0.23877242640699947
2304	92-93	0.1937348011936635
2304	94-95	0.28241208637949455
2304	96-97	0.3017257758341785
2304	98-99	0.5592795308657728
2304	100-101	0.539508233066865
1102	1	1.3733702109306094
1102	2	0.1686462513160265
1102	3	0.04616724303982522
1102	4	0.1399389714061101
1102	5	0.19174323838708318
1102	6	0.2458284799953674
1102	7	-4.887565540088158
1102	8	-1.2736984603798476
1102	9	0.1171967648885115
1102	10-11	0.6625799254131692
1102	12-13	0.9289432166271041
1102	14-15	0.9111000118219224
1102	16-17	0.6453775418649528
1102	18-19	0.8342795596184587
1102	20-21	1.045181945488757
1102	22-23	0.6470164460748578
1102	24-25	0.9428332806655817
1102	26-27	0.9964156474699095
1102	28-29	0.7362628340527806
1102	30-31	1.062476259562601
1102	32-33	0.6849087704189643
1102	34-35	1.0883099731929207
1102	36-37	1.1835529326188592
1102	38-39	0.7865206534558595
1102	40-41	1.03549813827631
1102	42-43	1.0785252089386077
1102	44-45	1.1084279112197564
1102	46-47	1.0630128096141789
1102	48-49	0.9491006196660052
1102	50-51	0.864659725577198
1102	52-53	1.1561976267170309
1102	54-55	1.4925509387807097
1102	56-57	1.1847132777184015
1102	58-59	0.9872786331399759
1102	60-61	0.9533793327791287
1102	62-63	0.6287207994727595
1102	64-65	0.48412963423739086
1102	66-67	0.5952521311647914
1102	68-69	0.6591920116443291
1102	70-71	0.4547026567170924
1102	72-73	0.4782748492614495
1102	74-75	0.5284705980938114
1102	76-77	0.6090862201237535
1102	78-79	0.6483433264043867
1102	80-81	1.2116124574801717
1102	82-83	1.0490323187996964
1102	84-85	0.6465868708356517
1102	86-87	0.5171416283660086
1102	88-89	0.5739793743027803
1102	90-91	0.26209625099134115
1102	92-93	0.4677135863935078
1102	94-95	0.41239594970644333
1102	96-97	0.37111216707197414
1102	98-99	0.17273976410314873
1102	100-101	0.285763348847361
2202	1	1.4820822790981154
2202	2	0.9565351566974094
2202	3	0.38730275498482314
2202	4	-0.39648497452327547
2202	5	0.044827370578190084
2202	6	0.6325518282647948
2202	7	0.5821374538589268
2202	8	-3.9625045179495046
2202	9	-0.35099137875592845
2202	10-11	0.5261253009033453
2202	12-13	0.28320212095151476
2202	14-15	0.17011690068571284
2202	16-17	0.33196760620220545
2202	18-19	0.6595622776185763
2202	20-21	1.1308581773348791
2202	22-23	-2.913939584727352
2202	24-25	-2.9893951022870446
2202	26-27	-1.5654201030735422
2202	28-29	0.6148020795504863
2202	30-31	1.4664747202915365
2202	32-33	1.2595550223473495
2202	34-35	-1.7839707846595871
2202	36-37	-0.14199421642904753
2202	38-39	0.2307080545565512
2202	40-41	1.2070959542971735
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2202	60-61	1.4258361490522091
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2305	1	1.3642579918095414
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1103	3	0.22968438009144165
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1103	100-101	1.1644534570716054
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1209	88-89	-2.3571512080629162
1209	90-91	-6.432368183030395
1209	92-93	-3.6844599335842148
1209	94-95	-0.73335217679805
1209	96-97	0.2951803011946055
1209	98-99	0.6525402460227276
1209	100-101	-0.07755555582826901
1212	1	-28.632148117804114
1212	2	-16.331944998191265
1212	3	-5.819660349593388
1212	4	-1.4784799762852145
1212	5	-0.3746317341944376
1212	6	-2.850436340411566
1212	7	-0.3503539288384161
1212	8	-0.9987113656166571
1212	9	0.2913044893942782
1212	10-11	0.8738947257819376
1212	12-13	0.34080539694417666
1212	14-15	-0.25583809086912623
1212	16-17	-0.04012266181513979
1212	18-19	-0.938669909576376
1212	20-21	-0.8392040373625349
1212	22-23	-0.4272402040390233
1212	24-25	0.3231567939245821
1212	26-27	-0.12369170101106164
1212	28-29	0.7172892497943621
1212	30-31	-0.5292824479676241
1212	32-33	0.16160636567446574
1212	34-35	-1.2054804226721245
1212	36-37	-0.8296681320260646
1212	38-39	-0.09117604626690223
1212	40-41	-0.12357354227412998
1212	42-43	-0.27681203417296985
1212	44-45	1.4402248479370954
1212	46-47	-1.355942715558868
1212	48-49	-0.8842733947119967
1212	50-51	0.5094639040702305
1212	52-53	-0.6595403301819509
1212	54-55	0.008045469104310143
1212	56-57	-0.9613896706128209
1212	58-59	-0.05088994471117658
1212	60-61	0.48226479859632576
1212	62-63	-4.272557025124868
1212	64-65	-3.828617028954895
1212	66-67	-1.406589648158132
1212	68-69	-0.0551866486189212
1212	70-71	-0.4310901206382951
1212	72-73	-0.9145642698509953
1212	74-75	0.1405383551224233
1212	76-77	1.1673704750751597
1212	78-79	-1.038248668555564
1212	80-81	0.8996619608140932
1212	82-83	0.8217954248304409
1212	84-85	-3.4949980076901994
1212	86-87	-2.1551185445064434
1212	88-89	-0.49064576361420364
1212	90-91	0.30235729135958067
1212	92-93	-2.1846902510384396
1212	94-95	-1.834464738056095
1212	96-97	0.28596440417209834
1212	98-99	-0.6942282721403643
1212	100-101	-0.2080239959393353
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	11.0
11	5.0
12	43.0
13	144.0
14	447.0
15	991.0
16	1789.0
17	3166.0
18	4644.0
19	6857.0
20	9738.0
21	12519.0
22	16982.0
23	21420.0
24	27010.0
25	32906.0
26	40155.0
27	47806.0
28	56918.0
29	67339.0
30	77901.0
31	90973.0
32	104507.0
33	118277.0
34	131920.0
35	143180.0
36	146917.0
37	130626.0
38	47396.0
39	163.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	27.656682494317796	30.845841678481143	18.26338411683957	23.234091710361486
2	24.41131718826455	29.069018344367066	26.370353488974835	20.14931097839355
3	20.55539667786754	26.1423554014442	30.27751960153476	23.0247283191535
4	23.22222114649188	25.64775309307641	28.531446866409933	22.598578894021777
5	21.85555539833343	27.286917886110444	27.717896834646925	23.139629880909204
6	21.74384493992584	27.532904315523954	27.421640698949997	23.30161004560021
7	22.73426986416753	27.85008754376257	27.87116358358214	21.54447900848776
8	21.27063943811129	26.914773112335293	28.868067714411467	22.94651973514195
9	22.102286564136325	26.47597443171028	28.49175241685577	22.929986587297627
10-11	22.567672333862344	27.00980147562068	27.54515522912932	22.87737096138765
12-13	22.61744610905553	26.985054314371986	27.2696274509365	23.12787212563598
14-15	22.63697848418533	27.176073994056253	27.074231254885007	23.112716266873406
16-17	22.785386121477476	27.129117758988823	27.085066404716883	23.00042971481682
18-19	23.056151038501547	27.058007702546018	26.81552290444891	23.07031835450353
20-21	22.802467462442916	27.067426993437376	27.02590790880845	23.104197635311255
22-23	22.70001199025587	26.96522900271605	27.23694607438278	23.097812932645294
24-25	22.866646757380522	26.80864817684808	27.185298605779007	23.13940645999239
26-27	22.85901320938934	27.010211080634843	27.054932500817348	23.07584320915847
28-29	23.02203600502548	26.946982961176147	27.059587103250998	22.971393930547375
30-31	23.092748725197485	26.83966644746592	26.884425104467898	23.183159722868695
32-33	22.94328013184813	26.843874208065944	27.024882387505706	23.18796327258022
34-35	23.115351474615288	26.866923799317377	26.983698465172772	23.034026260894564
36-37	23.142310931910988	26.892356547014835	26.922332186687537	23.04300033438664
38-39	22.94603565648885	26.879547081117433	27.116038121566298	23.058379140827416
40-41	23.09906514910881	26.816014418413264	26.972449733843984	23.11247069863394
42-43	22.971244983269496	26.812520806072875	27.053778159413806	23.162456051243822
44-45	22.91956027784625	26.706358633766197	27.24856396205717	23.12551712633038
46-47	23.02242358237996	26.9099004339904	27.099706054560922	22.967969929068715
48-49	23.18931243304352	26.73206199336961	27.01074245404882	23.067883119538056
50-51	23.048620187599376	26.84354182259608	26.95413534114563	23.153702648658914
52-53	23.030746487631774	26.798894809552003	27.154171833283065	23.01618686953316
54-55	22.92087685960661	26.68170326951156	27.237811805632354	23.159608065249472
56-57	22.986585240972268	26.881166274122826	27.018944507023896	23.11330397788101
58-59	23.062360996860537	26.755842371604615	27.175530751119197	23.006265880415647
60-61	23.16717824822014	26.641402246093133	26.92652998756639	23.264889518120338
62-63	23.01124494223366	26.81407453581751	27.02293896719909	23.151741554749737
64-65	23.110296680479383	26.799215624357647	26.95341708348104	23.137070611681924
66-67	23.126175341384307	26.730270091122705	26.97381013558552	23.169744431907468
68-69	23.082339894442814	26.770473629508647	26.991001981478153	23.156184494570393
70-71	23.220499170333387	26.858036147844505	26.86421768545358	23.057246996368534
72-73	23.068071630978203	26.814270616927445	27.003059436814787	23.114598315279565
74-75	23.007938666259363	26.71195419341139	27.0404905507147	23.23961658961454
76-77	23.021380411540193	26.992214759693695	26.937162708277462	23.04924212048865
78-79	23.18210304422964	26.81545793773764	26.845806024709674	23.156632993323047
80-81	23.077057869086183	26.769522838036945	26.926744990664353	23.22667430221252
82-83	23.118598901682034	26.914301482211634	26.822545346485544	23.144554269620787
84-85	23.102996016185504	26.649998547684	26.946419966589286	23.30058546954121
86-87	23.016881730320566	26.82226274694833	26.938784782050913	23.22207074068019
88-89	23.14000682972861	26.875726868949272	26.84872821441326	23.135538086908856
90-91	23.192863974827702	26.70396475639662	26.714691588673656	23.388479680102023
92-93	23.01465853664895	26.757853159903217	26.86164458197232	23.365843721475514
94-95	23.17852628536825	26.79730334668352	26.6982383563256	23.325932011622633
96-97	23.205876602519343	26.84289026059269	26.599111806273218	23.352121330614754
98-99	23.08373827264954	26.8192848438985	26.737874910247967	23.35910197320399
100-101	23.231273313391572	26.712007364946196	26.602255663738962	23.454463657923274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	4.5
4	11.5
5	16.0
6	21.0
7	39.0
8	67.0
9	104.5
10	146.5
11	204.5
12	298.0
13	433.0
14	643.0
15	893.0
16	1184.5
17	1608.0
18	2193.0
19	2949.0
20	4027.0
21	5312.5
22	6768.5
23	8447.0
24	10310.0
25	12436.5
26	14905.0
27	17413.5
28	19937.5
29	22617.0
30	24933.5
31	27215.0
32	29741.5
33	31955.0
34	33930.5
35	35769.0
36	37634.0
37	38924.0
38	39549.5
39	39621.5
40	38598.5
41	37391.5
42	35945.5
43	34106.5
44	32593.5
45	31355.5
46	30128.5
47	29149.5
48	28274.0
49	27252.5
50	26252.5
51	25477.0
52	24991.5
53	24669.5
54	24881.0
55	25701.0
56	26306.5
57	27009.5
58	27885.5
59	28292.5
60	28471.5
61	28065.5
62	27022.0
63	25708.5
64	24120.5
65	22177.5
66	20020.5
67	17981.0
68	15955.0
69	13893.0
70	11898.5
71	10190.0
72	8714.0
73	7221.0
74	5833.0
75	4635.5
76	3540.0
77	2641.5
78	1966.0
79	1366.0
80	921.0
81	619.0
82	404.0
83	273.0
84	187.0
85	127.5
86	88.5
87	60.0
88	45.0
89	29.5
90	16.0
91	11.5
92	8.5
93	7.0
94	4.0
95	1.5
96	1.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7184471948386789
2	0.004170523780550018
3	3.723681946919659E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.1864819919017365
14-15	3.723681946919658E-5
16-17	4.46841833630359E-4
18-19	0.12228571513684158
20-21	7.447363893839316E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0021969723486825986
42-43	0.0
44-45	0.0
46-47	0.02465077448860814
48-49	3.723681946919658E-5
50-51	1.4894727787678633E-4
52-53	1.4894727787678633E-4
54-55	0.0024948669044361715
56-57	0.0014522359592986668
58-59	0.09573586285530442
60-61	0.001899077792929026
62-63	0.0014894727787678635
64-65	0.0024576300849669746
66-67	0.004617365614180377
68-69	0.005399338823033505
70-71	0.003649208307981265
72-73	0.03846563451168007
74-75	0.04408839425152876
76-77	0.16440055795650294
78-79	2.606577362843761E-4
80-81	0.12370071427667106
82-83	0.005026970628341539
84-85	0.005511049281441095
86-87	0.006888811601801369
88-89	0.007261179796493334
90-91	0.024501827210731353
92-93	0.011953019049612103
94-95	0.015192622343432207
96-97	0.010761440826597813
98-99	0.012586044980588445
100-101	0.013479728647849165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1342757.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.00669164523819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.32982731426969	96.3698106511915
2	1.425901228422188	2.7949572402107945
3	0.19239813682963058	0.5656891460813985
4	0.022513415462446455	0.0882586146843651
5	0.020645041541960635	0.10116761102030344
6	0.008627712558058855	0.050734413856885395
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	8.71509160096349E-5	0.02938232295472981
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.957891115071454E-4	0.0	0.0
2	6.702627504455385E-4	0.0	0.0
3	0.0010426309451375044	7.447363893839318E-5	0.0
4	0.0014149991398294702	7.447363893839318E-5	0.0
5	0.0020107882513366155	7.447363893839318E-5	0.0
6	0.0023086828070901884	7.447363893839318E-5	0.0
7	0.0029044719185973337	7.447363893839318E-5	1.4894727787678635E-4
8	0.003500261030104479	7.447363893839318E-5	1.4894727787678635E-4
9	0.0038726292247964447	7.447363893839318E-5	1.4894727787678635E-4
10-11	0.004393944697365197	7.447363893839318E-5	1.4894727787678635E-4
12-13	0.005064207447810736	7.447363893839318E-5	1.4894727787678635E-4
14-15	0.0056972333787870776	7.447363893839318E-5	2.2342091681517952E-4
16-17	0.006367496129232616	7.447363893839318E-5	2.2342091681517952E-4
18-19	0.00741012707437012	7.447363893839318E-5	2.2342091681517952E-4
20-21	0.007894205727469676	7.447363893839318E-5	2.2342091681517952E-4
22-23	0.008117626644284856	7.447363893839318E-5	2.2342091681517952E-4
24-25	0.008713415755792	7.447363893839318E-5	2.2342091681517952E-4
26-27	0.00949538896464513	7.447363893839318E-5	2.2342091681517952E-4
28-29	0.010426309451375043	7.447363893839318E-5	2.2342091681517952E-4
30-31	0.011692361313327727	7.447363893839318E-5	2.2342091681517952E-4
32-33	0.015155385523963009	7.447363893839318E-5	2.2342091681517952E-4
34-35	0.018246041539906326	7.447363893839318E-5	2.2342091681517952E-4
36-37	0.022379328500987148	7.447363893839318E-5	2.2342091681517952E-4
38-39	0.027778667324020654	7.447363893839318E-5	2.2342091681517952E-4
40-41	0.035300504856798365	7.447363893839318E-5	2.2342091681517952E-4
42-43	0.044758657001974296	7.447363893839318E-5	2.2342091681517952E-4
44-45	0.05615312375954845	7.447363893839318E-5	2.2342091681517952E-4
46-47	0.07022864151890476	7.447363893839318E-5	2.2342091681517952E-4
48-49	0.08631494752959767	7.447363893839318E-5	2.2342091681517952E-4
50-51	0.10515677818101116	7.447363893839318E-5	2.2342091681517952E-4
52-53	0.1277222907793443	7.447363893839318E-5	2.2342091681517952E-4
54-55	0.15233582844848326	7.447363893839318E-5	2.2342091681517952E-4
56-57	0.1845456772893383	7.447363893839318E-5	2.606577362843761E-4
58-59	0.2205536817160514	7.447363893839318E-5	2.978945557535727E-4
60-61	0.2633387872861582	7.447363893839318E-5	2.978945557535727E-4
62-63	0.30954968024743124	7.447363893839318E-5	3.351313752227693E-4
64-65	0.3608620174759841	7.447363893839318E-5	3.723681946919659E-4
66-67	0.418616324472708	7.447363893839318E-5	3.723681946919659E-4
68-69	0.4825891803207878	1.1171045840758977E-4	3.723681946919659E-4
70-71	0.5588129497742331	1.4894727787678635E-4	4.0960501416116243E-4
72-73	0.6409201367038116	1.4894727787678635E-4	5.213154725687522E-4
74-75	0.7322992916812201	1.4894727787678635E-4	5.213154725687522E-4
76-77	0.8321684414976053	1.4894727787678635E-4	5.585522920379489E-4
78-79	0.9420915325706736	1.4894727787678635E-4	5.957891115071454E-4
80-81	1.0742822416863216	1.4894727787678635E-4	5.957891115071454E-4
82-83	1.21663860251706	1.8618409734598294E-4	6.33025930976342E-4
84-85	1.369495746438112	2.2342091681517952E-4	6.702627504455385E-4
86-87	1.535236829895506	2.2342091681517952E-4	6.702627504455385E-4
88-89	1.7193729021706832	2.2342091681517952E-4	6.702627504455385E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTTCC	760	0.0021481323	6.893404	8
CGTCTGA	1920	0.0	5.0672927	94-95
TCGGAAG	3445	0.0	5.028368	94-95
>>END_MODULE