##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib14_AGTTCC_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1684969 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.856389049294084 27.0 22.0 33.0 2.0 33.0 2 24.87147063239739 27.0 15.0 33.0 2.0 33.0 3 25.764748194180427 33.0 22.0 33.0 6.0 33.0 4 27.8618140749177 33.0 22.0 37.0 2.0 37.0 5 27.896981487493242 33.0 15.0 37.0 6.0 37.0 6 28.95130889648415 33.0 22.0 37.0 15.0 37.0 7 28.609189249179064 33.0 27.0 37.0 6.0 37.0 8 28.36341202716489 33.0 22.0 37.0 2.0 37.0 9 29.088227735940542 33.0 27.0 37.0 15.0 37.0 10-11 28.661694666192673 33.0 24.5 37.0 10.5 37.0 12-13 28.92779214335694 33.0 27.0 37.0 10.5 37.0 14-15 29.185710241553405 33.0 24.5 37.0 6.0 40.0 16-17 30.00296592993699 33.0 27.0 37.0 15.0 40.0 18-19 30.24101274266767 33.0 27.0 37.0 15.0 40.0 20-21 30.206752765184405 33.0 27.0 37.0 15.0 40.0 22-23 30.162735931640285 33.0 27.0 37.0 15.0 40.0 24-25 29.665983469132073 33.0 27.0 37.0 10.5 40.0 26-27 29.062922225868846 33.0 22.0 37.0 6.0 40.0 28-29 29.173599336248916 33.0 22.0 37.0 15.0 40.0 30-31 28.989168643458722 33.0 22.0 37.0 10.5 40.0 32-33 28.726834440277536 33.0 22.0 37.0 6.0 40.0 34-35 28.727194684293895 33.0 22.0 37.0 10.5 40.0 36-37 28.946287735857453 33.0 22.0 37.0 6.0 40.0 38-39 28.899832578522215 33.0 22.0 37.0 10.5 40.0 40-41 28.88482429053591 33.0 22.0 37.0 10.5 40.0 42-43 28.685339611589292 33.0 22.0 37.0 15.0 40.0 44-45 28.325875728277495 33.0 22.0 37.0 10.5 40.0 46-47 27.870505629480423 33.0 22.0 37.0 6.0 40.0 48-49 27.611164953183113 33.0 22.0 37.0 6.0 40.0 50-51 26.730335394894507 30.0 22.0 35.0 6.0 38.5 52-53 26.42255940613744 27.0 22.0 35.0 6.0 37.0 54-55 27.286830499552217 33.0 22.0 37.0 6.0 38.5 56-57 27.125434948654842 33.0 22.0 37.0 6.0 40.0 58-59 27.25982050708351 33.0 22.0 37.0 6.0 40.0 60-61 26.978555095078903 30.0 22.0 37.0 6.0 38.5 62-63 26.78451562016868 27.0 22.0 37.0 6.0 37.0 64-65 26.724543300203152 27.0 22.0 37.0 6.0 37.0 66-67 26.54944898096048 27.0 22.0 33.0 6.0 37.0 68-69 26.227020497113003 27.0 22.0 33.0 6.0 37.0 70-71 26.103534545739418 27.0 22.0 33.0 6.0 37.0 72-73 25.830238419816624 27.0 22.0 33.0 6.0 37.0 74-75 25.74349913855982 27.0 22.0 33.0 6.0 37.0 76-77 25.355127305012736 27.0 15.0 33.0 6.0 37.0 78-79 24.76731738091324 27.0 15.0 33.0 6.0 37.0 80-81 24.64423054667475 27.0 15.0 33.0 6.0 37.0 82-83 24.20575630768281 27.0 15.0 33.0 6.0 37.0 84-85 24.2712824390241 27.0 15.0 33.0 6.0 37.0 86-87 23.964093404685784 27.0 15.0 33.0 6.0 37.0 88-89 23.586663018726163 27.0 15.0 33.0 6.0 37.0 90-91 23.413236682692677 27.0 15.0 33.0 6.0 37.0 92-93 23.262498597897054 27.0 15.0 33.0 6.0 37.0 94-95 23.02547643309758 27.0 15.0 33.0 6.0 37.0 96-97 22.856948110024575 27.0 15.0 33.0 6.0 33.0 98-99 22.57871687847076 27.0 15.0 33.0 6.0 33.0 100-101 22.067603320891955 24.5 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.469171870269818 2306 2 8.645528900275988 2306 3 7.656047671921598 2306 4 9.295974211653643 2306 5 9.075762357981397 2306 6 8.083998346444353 2306 7 8.222692895048095 2306 8 8.684946561284992 2306 9 7.675060986054092 2306 10-11 7.982235033761089 2306 12-13 7.591204838462499 2306 14-15 8.25674624543878 2306 16-17 6.921878088407496 2306 18-19 6.219456657588818 2306 20-21 6.008726987381994 2306 22-23 5.715853311107139 2306 24-25 5.82276833062334 2306 26-27 6.317773785622784 2306 28-29 5.342031887984575 2306 30-31 5.111876597469109 2306 32-33 5.1273807587880995 2306 34-35 4.267576068159393 2306 36-37 4.45642160517772 2306 38-39 3.9783017111510617 2306 40-41 3.176111507567292 2306 42-43 2.728331706620395 2306 44-45 2.4642595202039246 2306 46-47 2.897221755312952 2306 48-49 2.628411447283792 2306 50-51 2.454561322075712 2306 52-53 3.110893546938758 2306 54-55 2.428811630375723 2306 56-57 2.7672713912613 2306 58-59 2.187590743149478 2306 60-61 2.239427213248735 2306 62-63 1.8716753828094603 2306 64-65 1.4317189532131636 2306 66-67 1.2594867164910823 2306 68-69 1.5850697733534567 2306 70-71 1.5056969720287157 2306 72-73 0.6259168069921799 2306 74-75 0.24619279672331373 2306 76-77 0.5461570685138248 2306 78-79 0.9047421410519512 2306 80-81 0.6607126365971396 2306 82-83 0.6779786891716775 2306 84-85 0.3005886194957412 2306 86-87 0.08775207654265316 2306 88-89 -0.0015309773384650782 2306 90-91 -0.017945750460427234 2306 92-93 -0.041944811594994746 2306 94-95 0.12988489648174806 2306 96-97 -0.18179849403121295 2306 98-99 -0.5080020077549463 2306 100-101 -0.5349270337864063 2307 1 8.375358308128618 2307 2 8.477336346194704 2307 3 7.603490097690521 2307 4 9.328419714543045 2307 5 9.075989577785222 2307 6 7.60796394685115 2307 7 8.176194515585674 2307 8 8.547617882046882 2307 9 7.559288486244185 2307 10-11 7.877384975070068 2307 12-13 7.404960933299588 2307 14-15 8.249601741960081 2307 16-17 6.845820788622298 2307 18-19 6.286189777385044 2307 20-21 6.430804406069971 2307 22-23 5.861219631013618 2307 24-25 6.136947529526509 2307 26-27 6.710419705289745 2307 28-29 6.004338409579063 2307 30-31 5.692916842403154 2307 32-33 5.769768123109657 2307 34-35 5.448896877572771 2307 36-37 5.27377163416681 2307 38-39 4.793606019357352 2307 40-41 4.27327396289558 2307 42-43 3.825672819088556 2307 44-45 3.7674567256082483 2307 46-47 4.245274080618049 2307 48-49 3.9514946923033705 2307 50-51 3.660888190683856 2307 52-53 4.203506259842225 2307 54-55 3.721457668850423 2307 56-57 4.05590336171144 2307 58-59 3.732052312409639 2307 60-61 3.5957593985032688 2307 62-63 3.2619496029554504 2307 64-65 3.1293109282255216 2307 66-67 2.832151461244532 2307 68-69 2.8931666099785573 2307 70-71 2.564607641676666 2307 72-73 2.156296985821591 2307 74-75 1.8039795451455483 2307 76-77 1.9531064005767043 2307 78-79 2.3963008510850266 2307 80-81 2.0216838185915904 2307 82-83 2.063723364208748 2307 84-85 1.4529438047407695 2307 86-87 1.3221958365225035 2307 88-89 1.62722275322459 2307 90-91 1.3214009376549996 2307 92-93 1.1719582781191065 2307 94-95 1.5287912415279408 2307 96-97 1.2432525338933615 2307 98-99 1.0814830913782316 2307 100-101 0.7242793780297383 1101 1 8.150046171294981 1101 2 8.031880703345774 1101 3 7.453980644038683 1101 4 9.001688420100301 1101 5 8.75775778254917 1101 6 7.576307394364548 1101 7 7.346488411623785 1101 8 8.227011651533576 1101 9 7.6021092022417776 1101 10-11 7.80493071996894 1101 12-13 7.424076596788385 1101 14-15 7.68687342795204 1101 16-17 6.97686953250377 1101 18-19 6.2159445467799195 1101 20-21 6.02675003274879 1101 22-23 5.535247812267343 1101 24-25 5.941466041243004 1101 26-27 6.513488820238685 1101 28-29 5.8100569096195045 1101 30-31 5.651262135584634 1101 32-33 5.74295477701375 1101 34-35 5.452768143591619 1101 36-37 5.18954858441769 1101 38-39 4.776365518162688 1101 40-41 4.12635992298658 1101 42-43 4.016837359638828 1101 44-45 3.970172351028239 1101 46-47 4.148276012640299 1101 48-49 3.9258550330009285 1101 50-51 3.2408982065499146 1101 52-53 4.203800289668468 1101 54-55 4.108006549256604 1101 56-57 4.183496418958772 1101 58-59 3.777823208747556 1101 60-61 3.954597716153998 1101 62-63 3.503848047425162 1101 64-65 3.391468240567903 1101 66-67 3.0460250168648173 1101 68-69 3.204195130567637 1101 70-71 3.0295281654450363 1101 72-73 2.631418930663177 1101 74-75 2.116093802034573 1101 76-77 2.275185668828602 1101 78-79 2.7825172375770464 1101 80-81 2.4893211536293016 1101 82-83 2.621775554959001 1101 84-85 2.0681766629843423 1101 86-87 1.9332177893305627 1101 88-89 1.9745353753442636 1101 90-91 1.8718642246712847 1101 92-93 1.5220390983133285 1101 94-95 1.6670174146218102 1101 96-97 1.8332985600513716 1101 98-99 1.4528201851716176 1101 100-101 1.2724905394870802 2201 1 7.158009292600607 2201 2 7.990067450292589 2201 3 6.362616501073045 2201 4 8.51206396702527 2201 5 3.7844296639862343 2201 6 5.714773660085676 2201 7 6.587281218578905 2201 8 7.788694545442841 2201 9 6.44073615977225 2201 10-11 4.002011371628093 2201 12-13 6.53196316566925 2201 14-15 7.107603126575231 2201 16-17 6.155961198911214 2201 18-19 1.8165150102900505 2201 20-21 5.021823402443889 2201 22-23 5.01030033299682 2201 24-25 5.424321826034337 2201 26-27 5.983940987871044 2201 28-29 4.880989445613942 2201 30-31 5.023285448794827 2201 32-33 5.223905172711639 2201 34-35 4.591845918275602 2201 36-37 4.886254364391302 2201 38-39 4.239011576623245 2201 40-41 4.012059679672781 2201 42-43 3.8953924383118093 2201 44-45 3.6362561747952995 2201 46-47 3.900708050550822 2201 48-49 3.6498833900220156 2201 50-51 3.290212231576259 2201 52-53 4.076344902889048 2201 54-55 3.9244998033112672 2201 56-57 4.061258660328797 2201 58-59 3.602237059197954 2201 60-61 3.566868864994216 2201 62-63 3.233563589540001 2201 64-65 3.163206815175414 2201 66-67 2.7743057131352984 2201 68-69 3.146739387201105 2201 70-71 2.7828177194911277 2201 72-73 2.1961807266786586 2201 74-75 1.8503228644911829 2201 76-77 2.1804769663580608 2201 78-79 2.6561434477919796 2201 80-81 2.1901059866896304 2201 82-83 2.488622854494448 2201 84-85 2.0201597046117143 2201 86-87 1.8446223556182098 2201 88-89 1.8927675985283479 2201 90-91 1.7020943144022596 2201 92-93 1.5167655163350915 2201 94-95 1.5649732600329536 2201 96-97 1.5766709333510391 2201 98-99 1.5441683575867948 2201 100-101 1.037251083654187 2304 1 1.3835768751437065 2304 2 4.571455700568421 2304 3 5.571903420063428 2304 4 4.172468553374124 2304 5 6.440128333672877 2304 6 6.1917529690226445 2304 7 7.029120715810858 2304 8 6.497879880402817 2304 9 5.588581265998037 2304 10-11 6.634829332073512 2304 12-13 6.500913204796117 2304 14-15 6.709780621581093 2304 16-17 5.397204315256523 2304 18-19 5.173655055298095 2304 20-21 4.9147754635304075 2304 22-23 4.47303867878108 2304 24-25 4.700009462431559 2304 26-27 5.584630203924235 2304 28-29 4.953128931666797 2304 30-31 4.6531829898780295 2304 32-33 4.832364594994996 2304 34-35 4.58824211543611 2304 36-37 4.659913479492328 2304 38-39 4.300811363734223 2304 40-41 3.548216303777842 2304 42-43 2.993654087667064 2304 44-45 3.1146992172613146 2304 46-47 3.957718320744899 2304 48-49 3.7307199758264993 2304 50-51 3.341775361919751 2304 52-53 4.014423117731983 2304 54-55 3.601093676190274 2304 56-57 4.352835448620869 2304 58-59 3.496237039756032 2304 60-61 3.522242854621954 2304 62-63 3.244769426004293 2304 64-65 3.037220259262881 2304 66-67 3.0597838142430014 2304 68-69 3.107292704235629 2304 70-71 2.915464834067219 2304 72-73 2.7757586912068746 2304 74-75 2.2256374590542514 2304 76-77 2.319329455652852 2304 78-79 2.8119638725007157 2304 80-81 2.5533226036983976 2304 82-83 2.6018506506070374 2304 84-85 2.1322382402463234 2304 86-87 2.085331791267887 2304 88-89 2.1748805463608143 2304 90-91 2.2731576901999873 2304 92-93 2.116950700620187 2304 94-95 2.367672284534631 2304 96-97 2.2479343178976308 2304 98-99 2.1496288637571723 2304 100-101 1.8082563767168232 1102 1 7.100028621277431 1102 2 7.513586899337682 1102 3 6.135681825739862 1102 4 6.008892284447022 1102 5 6.384285051933585 1102 6 5.809271341614206 1102 7 6.732371511792596 1102 8 6.457301310394666 1102 9 4.819578991140137 1102 10-11 5.842082435692081 1102 12-13 5.8475209130898484 1102 14-15 6.002627493706104 1102 16-17 5.233112324460848 1102 18-19 5.242524466216974 1102 20-21 5.426652254079581 1102 22-23 5.180532707552246 1102 24-25 1.5475141115767919 1102 26-27 5.375829825436831 1102 28-29 4.786635993341449 1102 30-31 4.687544707581498 1102 32-33 4.867986116330805 1102 34-35 4.737960107355008 1102 36-37 4.691558479284726 1102 38-39 3.863405248059557 1102 40-41 3.975985522612177 1102 42-43 4.007502421732461 1102 44-45 -4.04075880276006 1102 46-47 0.9498647070861352 1102 48-49 -1.3612674326929657 1102 50-51 2.2414121713495945 1102 52-53 -0.5702933772823435 1102 54-55 2.354997974819458 1102 56-57 -1.027885185279974 1102 58-59 2.6076423599952747 1102 60-61 3.7567379825799776 1102 62-63 3.5082021232078127 1102 64-65 3.0437313571167337 1102 66-67 2.787805758645561 1102 68-69 3.2483041746766794 1102 70-71 3.2054420199303237 1102 72-73 2.81030375240514 1102 74-75 2.6920686637237203 1102 76-77 2.737214952286454 1102 78-79 -1.5389614767588107 1102 80-81 1.4761168547107175 1102 82-83 2.4772845890394635 1102 84-85 2.068548556213379 1102 86-87 2.1473112891383472 1102 88-89 2.26808449032195 1102 90-91 2.2983756154683874 1102 92-93 2.072060826906487 1102 94-95 2.2503423908039295 1102 96-97 2.2376426072525604 1102 98-99 2.3255324150267 1102 100-101 1.9241226911192335 2202 1 -20.6337376124888 2202 2 -7.638387292400868 2202 3 -0.7932969301902908 2202 4 1.392905641950808 2202 5 1.305339342649141 2202 6 1.5959862358401473 2202 7 3.89326936046659 2202 8 2.5396271283611327 2202 9 3.0530508866149795 2202 10-11 2.0036543225953416 2202 12-13 1.2588586753112594 2202 14-15 2.580559666262726 2202 16-17 1.625391239567449 2202 18-19 1.6301972172917907 2202 20-21 0.9934107287909306 2202 22-23 1.3126172814630301 2202 24-25 2.331540999432839 2202 26-27 3.470054738144807 2202 28-29 -1.2667385898564056 2202 30-31 0.8355616254805938 2202 32-33 1.5478857862893847 2202 34-35 1.5579166331289152 2202 36-37 2.9268236777310754 2202 38-39 2.4718832534447763 2202 40-41 1.960425263311123 2202 42-43 0.6851779072135429 2202 44-45 0.826979347378682 2202 46-47 -1.148851432498926 2202 48-49 0.827472303543086 2202 50-51 1.6621032323204759 2202 52-53 1.6912396541328683 2202 54-55 -0.7147056537943328 2202 56-57 0.969572206507312 2202 58-59 1.973930424736949 2202 60-61 2.1242186309884623 2202 62-63 -2.1201860434719606 2202 64-65 4.7478550964541455E-4 2202 66-67 0.4728659120415024 2202 68-69 1.3232010500153812 2202 70-71 -2.5925066086515898 2202 72-73 -4.42526056600159 2202 74-75 -1.182879534862181 2202 76-77 -0.12742786412366414 2202 78-79 1.600272400693033 2202 80-81 1.6211213211437574 2202 82-83 -0.6403203827628587 2202 84-85 -0.32820299039089207 2202 86-87 0.67074323819622 2202 88-89 1.2350440430694256 2202 90-91 1.3775669608600296 2202 92-93 1.4421170239140828 2202 94-95 1.711394634256763 2202 96-97 1.438356173945511 2202 98-99 1.401742124152463 2202 100-101 1.2384058551814832 2305 1 5.818702715263246 2305 2 7.074701120587278 2305 3 6.746067580585898 2305 4 7.756738192250182 2305 5 7.66382892570617 2305 6 5.8978023332445915 2305 7 2.6287653447735373 2305 8 5.777679246198325 2305 9 6.3907739166279 2305 10-11 4.543148262158692 2305 12-13 5.283264809378807 2305 14-15 5.610965569358047 2305 16-17 5.00685283404033 2305 18-19 4.279464043680246 2305 20-21 5.120321605746799 2305 22-23 5.036050067522751 2305 24-25 5.306820237110209 2305 26-27 6.017756625628685 2305 28-29 5.175956831629151 2305 30-31 5.293680496741867 2305 32-33 5.3104016452265626 2305 34-35 5.1849228643462695 2305 36-37 4.662893283893492 2305 38-39 4.493901518206702 2305 40-41 3.792660594363845 2305 42-43 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1209 32-33 -24.617635405005004 1209 34-35 -21.699655644150678 1209 36-37 -25.02983917068435 1209 38-39 -25.199365314711006 1209 40-41 -21.706664030372842 1209 42-43 -22.605243420187904 1209 44-45 -25.00597216083056 1209 46-47 -18.59907564142074 1209 48-49 -18.34810378392811 1209 50-51 -20.291729677378385 1209 52-53 -22.82956981513021 1209 54-55 -19.85749364211995 1209 56-57 -23.520309427704216 1209 58-59 -23.75058274822983 1209 60-61 -23.478110502268507 1209 62-63 -23.549930220638817 1209 64-65 -23.41189634851097 1209 66-67 -11.520868730579249 1209 68-69 -23.001541218025853 1209 70-71 -22.836301950385078 1209 72-73 -13.310369486907373 1209 74-75 -14.45362729755837 1209 76-77 -11.840723035093674 1209 78-79 -12.003981473723774 1209 80-81 -21.80480460478217 1209 82-83 -13.693473663889918 1209 84-85 -11.354069879503438 1209 86-87 -11.175644182328242 1209 88-89 -9.364545318617694 1209 90-91 -11.401388027095857 1209 92-93 -15.426283773586626 1209 94-95 -20.32526057765618 1209 96-97 -16.294493682139663 1209 98-99 -7.9231165048434775 1209 100-101 -4.286777980015135 1212 1 -20.6337376124888 1212 2 -20.41158316868953 1212 3 -21.33587043152668 1212 4 -23.30774346076015 1212 5 -23.469765659259984 1212 6 -24.498706394934956 1212 7 -24.066639001503628 1212 8 -23.6932861973357 1212 9 -24.574669617394186 1212 10-11 -24.263050526583736 1212 12-13 -24.501736971882327 1212 14-15 -24.773964961457775 1212 16-17 -25.74555186848906 1212 18-19 -20.256364326150077 1212 20-21 -26.018616762618006 1212 22-23 -26.151872981996306 1212 24-25 -22.360632607128935 1212 26-27 -24.753885697135836 1212 28-29 -19.58353627745194 1212 30-31 -14.34228364212263 1212 32-33 -24.617635405005004 1212 34-35 -20.729407411254744 1212 36-37 -25.02983917068435 1212 38-39 -25.199365314711006 1212 40-41 -19.721906130248417 1212 42-43 -15.646943546378104 1212 44-45 -12.62720961902454 1212 46-47 -16.08702800682521 1212 48-49 -18.641562189695797 1212 50-51 -10.773524755078018 1212 52-53 -4.119703594394423 1212 54-55 -2.9411608353145056 1212 56-57 -3.3229850129884966 1212 58-59 -1.8701479656211362 1212 60-61 -12.731454983873858 1212 62-63 -10.453776374484972 1212 64-65 -14.62845153245746 1212 66-67 -10.718263741212759 1212 68-69 -7.039166636086055 1212 70-71 -13.572087903562336 1212 72-73 -10.145307406622166 1212 74-75 -7.131074549171046 1212 76-77 -17.025938043785796 1212 78-79 -8.599961036205574 1212 80-81 -6.283065474347389 1212 82-83 -4.111319870683008 1212 84-85 -3.2777733413507555 1212 86-87 -2.2708052733881523 1212 88-89 -2.0890226882137988 1212 90-91 -2.3775685232086694 1212 92-93 -2.573063717286381 1212 94-95 -2.308538169629422 1212 96-97 -11.51186513138561 1212 98-99 -8.534774979442183 1212 100-101 -4.960984437776716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 265.0 3 617.0 4 381.0 5 1946.0 6 2253.0 7 2603.0 8 5336.0 9 9359.0 10 13247.0 11 17499.0 12 23010.0 13 28546.0 14 32201.0 15 35586.0 16 38029.0 17 41733.0 18 45712.0 19 49667.0 20 53886.0 21 58033.0 22 61636.0 23 65400.0 24 66713.0 25 67579.0 26 69344.0 27 70109.0 28 70025.0 29 69783.0 30 71514.0 31 74584.0 32 78253.0 33 84345.0 34 90190.0 35 92058.0 36 90339.0 37 76851.0 38 26276.0 39 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.77430322320626 19.308993079596696 15.083723445665017 25.832980251532028 2 27.297962303176632 26.587196869131375 21.868379790184235 24.246461037507757 3 22.359714465518106 24.032595272513753 26.24072157754905 27.366968684419096 4 25.97024105125317 23.825462258272466 24.017377331142324 26.186919359332038 5 24.700766678109623 25.149919081947917 23.954162784234875 26.19515145570758 6 24.3136253432187 25.54055376800173 23.788067785379763 26.357753103399812 7 26.267022155780033 23.880564606549353 24.56664894485006 25.285764292820556 8 25.359865975935385 24.32991270731517 24.681620179711686 25.628601137037755 9 24.906943216524674 23.947742525317473 25.412225225044203 25.73308903311365 10-11 26.04328418457121 23.965832314135202 24.068254195254156 25.922629306039436 12-13 25.80858684232568 24.182143124913928 23.94410248672158 26.065167546038808 14-15 25.811058594802905 24.44587317861766 23.98421968091295 25.758848545666485 16-17 26.11800147363079 24.262330678846077 23.733842516688135 25.885825330834994 18-19 26.01727053680501 24.305080943382233 23.73981656797117 25.937831951841584 20-21 25.91434301797106 24.479419723865515 23.894753310083836 25.71148394807959 22-23 26.09898575896536 24.186447583251393 23.727389409130314 25.987177248652937 24-25 25.821535304271652 24.273724527145824 24.00301681765109 25.901723350931437 26-27 25.761269148713932 24.42378089379652 23.972198570817067 25.842751386672486 28-29 26.09539754109128 24.265393998856702 23.757281134143184 25.881927325908833 30-31 25.94432533355519 24.30409703335446 23.802819168010522 25.94875846507983 32-33 25.857341739247847 24.420251228294916 23.82777325830429 25.894633774152947 34-35 26.197854422746925 24.221666441541167 23.69850708479034 25.88197205092157 36-37 25.95949062444091 24.271988020459332 23.761975251759065 26.006546103340693 38-39 25.922059585137507 24.415504064455927 23.93649018665532 25.725946163751246 40-41 26.233221212080277 24.203481312374013 23.670012292812082 25.893285182733628 42-43 26.063209833516048 24.333351037831726 23.71189330513345 25.891545823518776 44-45 25.896435549415337 24.37011559721922 23.931472281958698 25.801976571406747 46-47 26.2753314730235 24.21459492515824 23.544094880616743 25.96597872120151 48-49 26.017223642193656 24.256889967946925 23.800504936630393 25.92538145322903 50-51 26.089563161959166 24.30774044237817 23.74917636590634 25.85352002975633 52-53 26.22779849951627 24.240053058467755 23.63642171746366 25.895726724552322 54-55 26.083779311587552 24.205485494734276 23.67941205548684 26.031323138191333 56-57 25.982214601367282 24.377864748500702 23.824036044816594 25.815884605315425 58-59 26.400621255636985 24.212660676188285 23.42137971929543 25.965338348879296 60-61 26.173938668418934 24.17142284367662 23.597099607455483 26.05753888044896 62-63 26.180814386045785 24.383932015472308 23.574908015243018 25.860345583238892 64-65 26.549502441146718 24.156099982302475 23.347151825233787 25.947245751317023 66-67 26.442286602042802 24.156000884517102 23.486226869086995 25.915485644353105 68-69 26.36145081427554 24.313430349027364 23.52250187180324 25.802616964893854 70-71 26.713176421856026 24.101678445467982 23.227102822506318 25.95804231016967 72-73 26.566185963450238 24.154215476253764 23.329732786351144 25.949865773944854 74-75 26.616232864077364 24.251476283369172 23.336045434980406 25.796245417573054 76-77 26.962363836419772 24.136528908965325 23.02185145802088 25.87925579659402 78-79 26.802033922258943 24.14611974414882 23.178802989681277 25.873043343910958 80-81 26.917978749935273 24.240858668796715 23.115769462213017 25.725393119055003 82-83 27.11623728605285 24.089704946352526 22.998054768322966 25.796002999271657 84-85 27.274125564732483 24.065242469367483 22.827381526807997 25.83325043909203 86-87 27.17592383568545 24.251741214323225 22.962226758264084 25.610108191727242 88-89 27.459731462900855 24.05126860332614 22.826752260850018 25.662247672922987 90-91 27.614872877945423 24.014771777873282 22.609557996068265 25.76079734811303 92-93 27.605026667423655 24.194931539790684 22.637511195026956 25.562530597758702 94-95 28.04253875628152 23.978845088744627 22.339762755456405 25.63885339951745 96-97 28.003158389793075 24.019131430892948 22.381349654659086 25.596360524654887 98-99 28.105480923324798 24.16163083458468 22.338962003433206 25.393926238657315 100-101 28.895553852962593 23.793406206427235 21.737277347635114 25.573762592975058 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 98.0 1 99.5 2 109.0 3 118.0 4 115.0 5 129.5 6 163.5 7 200.0 8 258.0 9 337.5 10 425.0 11 566.5 12 769.5 13 983.5 14 1263.0 15 1619.0 16 2062.5 17 2599.5 18 3223.0 19 3957.5 20 4815.5 21 5793.0 22 7026.5 23 8403.5 24 9940.5 25 11814.5 26 13722.5 27 15610.0 28 17784.0 29 20053.5 30 22098.5 31 24195.0 32 26370.0 33 28305.0 34 29863.5 35 31459.5 36 33115.5 37 34233.5 38 35080.0 39 35603.5 40 35648.0 41 35176.0 42 34529.5 43 33843.0 44 33305.0 45 32914.5 46 32238.0 47 31782.5 48 31303.0 49 30958.0 50 30528.0 51 30066.0 52 30170.5 53 30299.5 54 30585.0 55 31331.5 56 32201.0 57 33169.5 58 34195.5 59 35104.5 60 36086.5 61 37207.5 62 38010.5 63 38515.0 64 38602.5 65 38248.5 66 37913.0 67 37358.5 68 36040.5 69 34211.0 70 32123.5 71 29594.0 72 26895.5 73 23902.5 74 20723.5 75 17698.0 76 14831.5 77 12273.5 78 10002.0 79 8107.0 80 6593.5 81 5356.0 82 4376.5 83 3521.5 84 2832.0 85 2297.5 86 1837.5 87 1439.0 88 1144.5 89 896.0 90 684.5 91 540.5 92 428.0 93 329.5 94 245.0 95 179.5 96 111.0 97 61.5 98 36.5 99 19.0 100 9.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.855685178777769 2 11.1653092727522 3 9.843089101342517 4 11.804074733719137 5 9.237202583549015 6 5.414520979317721 7 9.803444455061191 8 10.161077147413394 9 5.483364975854156 10-11 6.418960823611592 12-13 5.408022343437772 14-15 7.414053314927456 16-17 2.69921879868413 18-19 3.176319564336198 20-21 3.075160433218653 22-23 3.1284848563979515 24-25 3.526177632941615 26-27 4.402959342278701 28-29 1.7350170834003475 30-31 2.2717035150201577 32-33 3.0809172156876476 34-35 2.020096512161351 36-37 3.136734266327749 38-39 1.3905597076266685 40-41 1.1490715852932605 42-43 0.552651117023518 44-45 1.3259887867373226 46-47 1.400441195060562 48-49 1.2931988659732019 50-51 1.5526695149881096 52-53 2.4628939760909545 54-55 1.8521112257851629 56-57 1.8414582108038782 58-59 1.5663492918860822 60-61 1.4174444752396038 62-63 0.6074295728882846 64-65 1.2400524876125318 66-67 0.5625326044574114 68-69 0.7983826408675768 70-71 0.9963684791827031 72-73 0.0847790078037044 74-75 0.10519481367312988 76-77 0.45772355455797703 78-79 0.8956841342481672 80-81 0.289619571636036 82-83 0.5081399123663403 84-85 0.06507538120879375 86-87 0.09857748124742947 88-89 0.08104006661250149 90-91 0.08833990417627861 92-93 0.16976573456247562 94-95 0.452708625499935 96-97 0.37184660370606226 98-99 0.06644039148494721 100-101 0.2298558608496655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1684969.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.64685723855646 #Duplication Level Percentage of deduplicated Percentage of total 1 99.68764832591177 99.33560861179552 2 0.2766259388769252 0.5512981087950122 3 0.02940886532338242 0.08791503013281066 4 0.006316869887919615 0.025178249276642483 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.7804481862871067E-4 0.0 0.0 2 2.3739309150494758E-4 0.0 0.0 3 3.5608963725742134E-4 0.0 0.0 4 4.7478618300989515E-4 0.0 0.0 5 7.715275473910796E-4 0.0 0.0 6 9.495723660197903E-4 0.0 0.0 7 0.0013056620032772116 0.0 0.0 8 0.0014837068219059222 0.0 0.0 9 0.0017804481862871068 0.0 0.0 10-11 0.001988167141353936 0.0 0.0 12-13 0.0024926274608019494 0.0 0.0 14-15 0.002819042961621252 0.0 2.9674136438118447E-5 16-17 0.0029674136438118445 0.0 5.9348272876236894E-5 18-19 0.003175132598878674 0.0 5.9348272876236894E-5 20-21 0.0034125256903836214 0.0 5.9348272876236894E-5 22-23 0.0035608963725742137 0.0 5.9348272876236894E-5 24-25 0.0039169860098316345 0.0 5.9348272876236894E-5 26-27 0.004629165284346478 0.0 5.9348272876236894E-5 28-29 0.005608411786804386 2.9674136438118447E-5 5.9348272876236894E-5 30-31 0.0076856013374726785 5.9348272876236894E-5 5.9348272876236894E-5 32-33 0.011632261483742431 5.9348272876236894E-5 8.902240931435535E-5 34-35 0.016558168132470092 5.9348272876236894E-5 1.1869654575247379E-4 36-37 0.024599859107200195 5.9348272876236894E-5 1.1869654575247379E-4 38-39 0.035697986135056495 5.9348272876236894E-5 1.1869654575247379E-4 40-41 0.04955580785165781 5.9348272876236894E-5 1.1869654575247379E-4 42-43 0.06860660344492986 5.9348272876236894E-5 1.7804481862871067E-4 44-45 0.09237558673186272 5.9348272876236894E-5 1.7804481862871067E-4 46-47 0.12216841971573364 5.9348272876236894E-5 1.7804481862871067E-4 48-49 0.16291100904527028 5.9348272876236894E-5 1.7804481862871067E-4 50-51 0.21021158252763106 5.9348272876236894E-5 1.7804481862871067E-4 52-53 0.26789810376333334 5.9348272876236894E-5 1.7804481862871067E-4 54-55 0.3349913262499191 5.9348272876236894E-5 1.7804481862871067E-4 56-57 0.4124704964898464 5.9348272876236894E-5 1.7804481862871067E-4 58-59 0.5048757573581473 1.1869654575247379E-4 1.7804481862871067E-4 60-61 0.6086462124822474 1.1869654575247379E-4 1.7804481862871067E-4 62-63 0.7207550999454588 1.1869654575247379E-4 1.7804481862871067E-4 64-65 0.8483835607658063 1.1869654575247379E-4 1.7804481862871067E-4 66-67 0.9884751588901636 1.1869654575247379E-4 1.7804481862871067E-4 68-69 1.1487451697924413 1.1869654575247379E-4 1.7804481862871067E-4 70-71 1.3198759146310703 2.0771895506682914E-4 1.7804481862871067E-4 72-73 1.4980987780784099 2.3739309150494758E-4 1.7804481862871067E-4 74-75 1.6923160010658949 2.3739309150494758E-4 1.7804481862871067E-4 76-77 1.9017857301825731 2.3739309150494758E-4 1.7804481862871067E-4 78-79 2.128733525661303 2.3739309150494758E-4 1.7804481862871067E-4 80-81 2.377313766603421 2.3739309150494758E-4 1.7804481862871067E-4 82-83 2.6430753325432104 2.3739309150494758E-4 1.7804481862871067E-4 84-85 2.9167005446391006 2.3739309150494758E-4 1.7804481862871067E-4 86-87 3.2144211555227424 2.3739309150494758E-4 1.7804481862871067E-4 88-89 3.526949160489006 2.9674136438118446E-4 1.7804481862871067E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATACG 390 8.4111925E-5 12.5587845 3 >>END_MODULE