##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib14_AGTTCC_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1684969 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.235145572411124 33.0 33.0 33.0 27.0 33.0 2 31.6215971925893 33.0 33.0 33.0 27.0 33.0 3 31.791455510457464 33.0 33.0 33.0 27.0 33.0 4 35.617874275431774 37.0 37.0 37.0 33.0 37.0 5 35.132452881922454 37.0 37.0 37.0 33.0 37.0 6 34.89779515231437 37.0 33.0 37.0 33.0 37.0 7 34.689799634295944 37.0 33.0 37.0 27.0 37.0 8 34.717855936815454 37.0 33.0 37.0 27.0 37.0 9 34.60868182144597 37.0 33.0 37.0 27.0 37.0 10-11 34.450513036144876 37.0 33.0 37.0 27.0 37.0 12-13 34.442133356756116 37.0 33.0 37.0 27.0 37.0 14-15 35.31346897183272 37.0 33.0 40.0 27.0 40.0 16-17 35.19677810096209 37.0 33.0 40.0 27.0 40.0 18-19 35.3923039533665 37.0 33.0 40.0 27.0 40.0 20-21 35.00329798352374 37.0 33.0 40.0 27.0 40.0 22-23 35.00321222526942 37.0 33.0 40.0 27.0 40.0 24-25 34.788812138383555 37.0 33.0 40.0 27.0 40.0 26-27 34.31191404708336 37.0 33.0 40.0 27.0 40.0 28-29 33.86429097508619 37.0 33.0 37.0 22.0 40.0 30-31 33.681889103004266 37.0 33.0 37.0 22.0 40.0 32-33 33.61815974062431 37.0 33.0 37.0 22.0 40.0 34-35 33.38545367896976 37.0 33.0 37.0 22.0 40.0 36-37 32.961693360530674 37.0 33.0 37.0 22.0 40.0 38-39 32.76033950772982 37.0 33.0 37.0 22.0 40.0 40-41 32.361663330304594 37.0 33.0 37.0 22.0 40.0 42-43 31.85863834883609 35.0 27.0 37.0 15.0 40.0 44-45 31.62315360104548 33.0 27.0 37.0 15.0 40.0 46-47 31.41541713823815 33.0 27.0 37.0 18.5 40.0 48-49 31.389645744224374 33.0 27.0 37.0 18.5 40.0 50-51 31.730295631551677 33.0 27.0 37.0 22.0 40.0 52-53 31.55519923511946 33.0 27.0 37.0 18.5 40.0 54-55 31.148286407643106 33.0 27.0 37.0 15.0 40.0 56-57 31.16045577099638 33.0 27.0 37.0 15.0 40.0 58-59 30.84926636632484 33.0 27.0 37.0 15.0 40.0 60-61 30.73839607731656 33.0 27.0 37.0 15.0 40.0 62-63 30.701573144669133 33.0 27.0 37.0 15.0 40.0 64-65 30.350251547654587 33.0 27.0 37.0 15.0 37.0 66-67 29.98658461965769 33.0 27.0 37.0 15.0 37.0 68-69 29.796846410824177 33.0 27.0 37.0 15.0 37.0 70-71 29.648314598072723 33.0 27.0 37.0 15.0 37.0 72-73 29.431256302044726 33.0 27.0 37.0 15.0 37.0 74-75 29.09148625286281 33.0 27.0 37.0 15.0 37.0 76-77 27.950530543885378 33.0 22.0 33.0 15.0 37.0 78-79 28.935062603525644 33.0 27.0 35.0 15.0 37.0 80-81 28.681391170994836 33.0 27.0 37.0 15.0 37.0 82-83 28.670912639935807 33.0 27.0 35.0 15.0 37.0 84-85 28.562363461879713 33.0 24.5 33.0 15.0 37.0 86-87 28.365353605912038 33.0 22.0 33.0 15.0 37.0 88-89 28.244146924958265 33.0 22.0 33.0 15.0 37.0 90-91 28.144743909235125 33.0 22.0 33.0 15.0 37.0 92-93 27.923676934115704 33.0 22.0 33.0 15.0 37.0 94-95 27.809934188700208 33.0 22.0 33.0 15.0 37.0 96-97 27.65532244213395 33.0 22.0 33.0 15.0 37.0 98-99 27.3624847104012 33.0 22.0 33.0 15.0 37.0 100-101 26.493684453541874 30.0 22.0 33.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.190310564974137 2306 2 0.6071916886862212 2306 3 0.4905010430496297 2306 4 0.24151046092806894 2306 5 0.6391328899560094 2306 6 0.7423152038375775 2306 7 0.9671214169753881 2306 8 0.8709889044555723 2306 9 1.0772337668188285 2306 10-11 1.21402902824898 2306 12-13 1.4668014581829354 2306 14-15 1.49869490795583 2306 16-17 1.5314212947965302 2306 18-19 1.3741281090893693 2306 20-21 1.6327544020879898 2306 22-23 1.2431260024364121 2306 24-25 1.1403639757472348 2306 26-27 1.2526698154345013 2306 28-29 1.5865460484768832 2306 30-31 1.4400911179669365 2306 32-33 1.0756365194561965 2306 34-35 0.948375535468891 2306 36-37 1.173389649184763 2306 38-39 1.0871937559833285 2306 40-41 1.0727673170901468 2306 42-43 1.0160371243371245 2306 44-45 0.6763219323962097 2306 46-47 0.7318627969910736 2306 48-49 0.7812725088958992 2306 50-51 0.934783235467183 2306 52-53 0.9076159165013351 2306 54-55 1.3209456346690125 2306 56-57 0.9980894146401056 2306 58-59 0.8959060891603485 2306 60-61 0.6843483570445699 2306 62-63 0.319170938594727 2306 64-65 0.22447861589762397 2306 66-67 0.563303665825714 2306 68-69 0.4283824934159064 2306 70-71 0.37246478280621176 2306 72-73 0.16115372246090942 2306 74-75 0.3682147257810726 2306 76-77 0.38562433153510867 2306 78-79 0.26849759643006976 2306 80-81 0.8525695883189393 2306 82-83 0.7364831204500106 2306 84-85 0.5093764948683059 2306 86-87 0.21226920035235608 2306 88-89 0.08379128874326014 2306 90-91 -0.2658984359023471 2306 92-93 -0.4848464012725273 2306 94-95 -0.5020595216796977 2306 96-97 -0.4923414856189794 2306 98-99 -0.3405852287558133 2306 100-101 -0.3025338037290197 2307 1 0.6989740254159109 2307 2 0.4634661036769039 2307 3 0.38835876127568625 2307 4 0.3736989232707657 2307 5 0.4644338299628572 2307 6 0.6431953909835286 2307 7 0.8194537911342294 2307 8 0.8338466345624056 2307 9 1.0002442717992537 2307 10-11 1.2208569090990693 2307 12-13 1.399651620236689 2307 14-15 1.3627299930696921 2307 16-17 1.5056458069719554 2307 18-19 1.287369647602084 2307 20-21 1.49949355137705 2307 22-23 1.341585689781958 2307 24-25 1.1636627677101288 2307 26-27 1.4169424566256197 2307 28-29 1.5344920705561194 2307 30-31 1.600315682417751 2307 32-33 1.2320816611700707 2307 34-35 1.244426158259067 2307 36-37 1.131582987397131 2307 38-39 0.8854754396315769 2307 40-41 0.9673498029282683 2307 42-43 1.0387509366874212 2307 44-45 0.8341525208747136 2307 46-47 0.7141444046023722 2307 48-49 0.7406656567782726 2307 50-51 0.7118522425671365 2307 52-53 1.1388878068275332 2307 54-55 1.3006963403869953 2307 56-57 1.034243649660823 2307 58-59 1.0868684437396716 2307 60-61 0.747194978627661 2307 62-63 0.5947383643636464 2307 64-65 0.7374103929287656 2307 66-67 1.0266545965655531 2307 68-69 0.7926191061597017 2307 70-71 0.644576388421541 2307 72-73 0.5696215659578456 2307 74-75 0.6054347256385029 2307 76-77 0.7314608924787329 2307 78-79 0.7570669552009335 2307 80-81 1.2149480480003447 2307 82-83 0.8676594893527998 2307 84-85 0.7317658729810717 2307 86-87 0.6813548322194407 2307 88-89 0.5895291085737 2307 90-91 0.2674369369638647 2307 92-93 0.36405138961634975 2307 94-95 0.2947193925601219 2307 96-97 0.1879609929481063 2307 98-99 0.41985604101379437 2307 100-101 0.4960260385418209 1101 1 1.1155892039227275 1101 2 0.5590226045334532 1101 3 0.38644695927725436 1101 4 0.36234365557591275 1101 5 0.5325104005595094 1101 6 0.48179536507728216 1101 7 0.7595088023815535 1101 8 0.6313680269806312 1101 9 1.0189203348523819 1101 10-11 1.0822110517573549 1101 12-13 1.3013748873915247 1101 14-15 1.3751291882732701 1101 16-17 1.2490848673900672 1101 18-19 0.9443966259788823 1101 20-21 1.2248129097291667 1101 22-23 0.9501336263736775 1101 24-25 1.0728305842452457 1101 26-27 1.086066770733396 1101 28-29 1.3869516589386848 1101 30-31 1.2967130872928792 1101 32-33 0.8524571527654388 1101 34-35 0.8136513303744621 1101 36-37 1.0703244424403735 1101 38-39 0.8864913932581828 1101 40-41 0.9554331571913366 1101 42-43 1.0095525130764997 1101 44-45 0.7970321675037617 1101 46-47 0.7554481456226014 1101 48-49 0.8730775192854239 1101 50-51 0.8484646723359681 1101 52-53 1.0836549729090805 1101 54-55 1.4916475040700838 1101 56-57 1.0534351010856966 1101 58-59 1.1199059786082017 1101 60-61 0.8894892991390257 1101 62-63 0.6941947132490434 1101 64-65 0.6912983368545085 1101 66-67 0.8365097344250998 1101 68-69 0.67658245490097 1101 70-71 0.7543265938167281 1101 72-73 0.5845483686465016 1101 74-75 0.7124532206314811 1101 76-77 0.6970549178656391 1101 78-79 0.569739777540633 1101 80-81 1.274607444701008 1101 82-83 0.8911927457376834 1101 84-85 0.8321553180704697 1101 86-87 0.7158428280793068 1101 88-89 0.6719254182105203 1101 90-91 0.36893104633323404 1101 92-93 0.5546826020226412 1101 94-95 0.5325791838141463 1101 96-97 0.677079152801813 1101 98-99 0.697121150102813 1101 100-101 0.4774646836085985 2201 1 1.3596994090665326 2201 2 0.7585946999986497 2201 3 0.2801779302336165 2201 4 0.1342517987357752 2201 5 0.4317076243391611 2201 6 0.3992690440168687 2201 7 0.5250278907931545 2201 8 0.5620876273602846 2201 9 0.6567730431315013 2201 10-11 0.8103492269872987 2201 12-13 1.152947360687321 2201 14-15 1.1404168744098513 2201 16-17 0.9822661327583972 2201 18-19 0.7268938889831205 2201 20-21 1.1305725123545542 2201 22-23 0.9043554660445281 2201 24-25 0.7730166165004491 2201 26-27 1.0391964675357244 2201 28-29 1.356757645201732 2201 30-31 1.3851218817932391 2201 32-33 0.8934474482851158 2201 34-35 0.7619538998501483 2201 36-37 0.9244804272998337 2201 38-39 0.7380494513302907 2201 40-41 0.8797938609880802 2201 42-43 0.8145575925461088 2201 44-45 0.7510973364539844 2201 46-47 0.6700767904929457 2201 48-49 0.9664733742257887 2201 50-51 0.9745074430633558 2201 52-53 1.0456168013967542 2201 54-55 1.4706794990384147 2201 56-57 1.0624255726228355 2201 58-59 1.0211107119607057 2201 60-61 0.7742631432467277 2201 62-63 0.858300806260619 2201 64-65 0.7816217006697173 2201 66-67 0.7352181303824636 2201 68-69 0.667824371943631 2201 70-71 0.8479216823224078 2201 72-73 0.6255538760206463 2201 74-75 0.7423888604511859 2201 76-77 0.6333580654676645 2201 78-79 0.7756004384710522 2201 80-81 1.2477163001246616 2201 82-83 1.1358409042687292 2201 84-85 0.8851242092590468 2201 86-87 0.8689924785860015 2201 88-89 0.9444282652172014 2201 90-91 0.6215464989978123 2201 92-93 0.7865271209373148 2201 94-95 0.585523075970066 2201 96-97 0.44204666923327096 2201 98-99 0.5105653946730158 2201 100-101 0.6623430001532569 2304 1 -2.0303516745774672 2304 2 -0.3885971577253784 2304 3 -0.1476643569138183 2304 4 0.012036003148118368 2304 5 0.371646673920921 2304 6 0.5232798148403788 2304 7 0.6339940181085666 2304 8 0.6360427456886697 2304 9 0.8582098009969741 2304 10-11 0.937739732525813 2304 12-13 1.184202190789243 2304 14-15 1.3079007497676969 2304 16-17 1.3184737919026617 2304 18-19 0.9796133275004806 2304 20-21 1.2534473890198754 2304 22-23 0.9560141048185216 2304 24-25 1.0807506121551782 2304 26-27 1.1980945481321967 2304 28-29 1.6063537393200633 2304 30-31 1.5622093364085075 2304 32-33 1.0384885436428704 2304 34-35 1.0609205618183495 2304 36-37 1.2694734039063391 2304 38-39 1.0414313732612612 2304 40-41 0.9414864745167364 2304 42-43 0.9894505404757865 2304 44-45 1.117275443880306 2304 46-47 0.9248082246747558 2304 48-49 0.8827292493308967 2304 50-51 1.018499403829562 2304 52-53 1.1470404359664244 2304 54-55 1.5064132613901045 2304 56-57 1.1687641244914495 2304 58-59 1.050418433805163 2304 60-61 0.5536342943541861 2304 62-63 0.28055451359167805 2304 64-65 0.32514049371534526 2304 66-67 0.375190717275693 2304 68-69 0.3709547640385473 2304 70-71 0.37787803521269225 2304 72-73 0.1949608002603469 2304 74-75 0.32312945177717367 2304 76-77 0.47292042785532473 2304 78-79 0.6070351063301551 2304 80-81 1.303875257260085 2304 82-83 0.9437384550728929 2304 84-85 0.6285287810264677 2304 86-87 0.5281165714989378 2304 88-89 0.37392433065869923 2304 90-91 0.19978272804306485 2304 92-93 0.2705113535404422 2304 94-95 -0.004269430141984998 2304 96-97 0.009316683474477117 2304 98-99 0.5689553120934612 2304 100-101 0.644405642018512 1102 1 1.3976096288002928 1102 2 0.07512683492339889 1102 3 0.244375388634257 1102 4 0.12282770177424851 1102 5 0.18508691027887636 1102 6 0.15569096754431655 1102 7 -5.35742445740885 1102 8 -1.2759010231455576 1102 9 0.15198289648637342 1102 10-11 0.555338632027798 1102 12-13 0.9121029624053065 1102 14-15 0.8466563740861659 1102 16-17 0.6976298873636608 1102 18-19 0.8944584688978665 1102 20-21 1.2104010024601166 1102 22-23 0.7283139402682863 1102 24-25 0.9708900537332923 1102 26-27 1.2282344486153605 1102 28-29 1.0481402129843858 1102 30-31 1.3039294873664105 1102 32-33 1.1185572045797798 1102 34-35 1.2139881235683916 1102 36-37 1.2573324151626295 1102 38-39 0.9762973402069832 1102 40-41 1.0038920985074142 1102 42-43 0.984305559258118 1102 44-45 0.9465875670591721 1102 46-47 0.8765349095665087 1102 48-49 1.0129929449151298 1102 50-51 0.8827749566462479 1102 52-53 0.928458496284037 1102 54-55 1.3168117220914475 1102 56-57 1.0892454940389484 1102 58-59 1.0955072756380702 1102 60-61 0.9078207103275773 1102 62-63 0.7859436978551706 1102 64-65 0.35093663934995334 1102 66-67 0.5692772886212296 1102 68-69 0.7884345667530788 1102 70-71 0.5417582741055504 1102 72-73 0.5526282295835081 1102 74-75 0.5538470263110042 1102 76-77 0.4773036266857744 1102 78-79 0.6105226754663846 1102 80-81 1.436970178492313 1102 82-83 1.1457432145738657 1102 84-85 0.9803401448267266 1102 86-87 0.6498255120619909 1102 88-89 0.5270667376375542 1102 90-91 0.2380538797417806 1102 92-93 0.3399539587225675 1102 94-95 0.3035263833327768 1102 96-97 0.07536426537264163 1102 98-99 0.08561586002609545 1102 100-101 0.2983500402082413 2202 1 1.5608405777455268 2202 2 0.8993696842030694 2202 3 0.4090567771742606 2202 4 -0.17379410221132474 2202 5 0.04428411410052746 2202 6 0.5107741650428252 2202 7 0.6463392473857823 2202 8 -4.452544730717104 2202 9 -0.6809547980124435 2202 10-11 0.4003247156000356 2202 12-13 -0.1056168402923987 2202 14-15 -0.01248628328917789 2202 16-17 0.21000307573095256 2202 18-19 0.742933222748178 2202 20-21 1.1270356142346358 2202 22-23 -3.0214018160748672 2202 24-25 -3.3132778048086493 2202 26-27 -1.628214889424548 2202 28-29 0.503494300886338 2202 30-31 1.323821407479251 2202 32-33 1.1607502975828865 2202 34-35 -2.004128947273273 2202 36-37 -0.39000833932097834 2202 38-39 0.1997179903581241 2202 40-41 1.1361842322723064 2202 42-43 1.2897350204313156 2202 44-45 1.2786376421136616 2202 46-47 0.5944940082325303 2202 48-49 0.8676305617029705 2202 50-51 -4.244260706103699 2202 52-53 -0.6157368763354896 2202 54-55 1.2319525050525506 2202 56-57 1.1309402308735983 2202 58-59 1.4688364365224658 2202 60-61 1.40208979148915 2202 62-63 1.1454981100605401 2202 64-65 1.249220976514998 2202 66-67 1.3667734296372487 2202 68-69 1.2620468378616678 2202 70-71 1.386621628173618 2202 72-73 1.1782904907391405 2202 74-75 0.6038128108128689 2202 76-77 1.2598365086295544 2202 78-79 1.3205298585958083 2202 80-81 1.958437392890307 2202 82-83 1.7205750946340252 2202 84-85 1.6936286154789642 2202 86-87 1.7952254402306949 2202 88-89 1.710269832475408 2202 90-91 1.4612703349169998 2202 92-93 1.439812208801733 2202 94-95 1.3963649662302693 2202 96-97 1.2248700533173071 2202 98-99 1.4472625180645053 2202 100-101 1.4123673434744433 2305 1 1.3432364399418901 2305 2 0.6928111337619534 2305 3 0.15246077196011143 2305 4 -0.04436640325384644 2305 5 0.23042501831737638 2305 6 0.32628026424689693 2305 7 0.5470999458686734 2305 8 -0.06477839681096498 2305 9 0.25008951614625374 2305 10-11 0.3544995115235494 2305 12-13 0.8377480334012901 2305 14-15 0.639579755181984 2305 16-17 0.7603549547200004 2305 18-19 0.6393174870914109 2305 20-21 0.76250790280357 2305 22-23 0.6192568057391199 2305 24-25 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-0.3819875689539316 1208 40-41 -0.7758172981157259 1208 42-43 0.32892960098458346 1208 44-45 0.1603073602937819 1208 46-47 -0.008486852139888867 1208 48-49 -1.7159089932186085 1208 50-51 -0.29195462643912506 1208 52-53 -1.4948737868865223 1208 54-55 -0.8209871367316737 1208 56-57 -6.074547810589937 1208 58-59 -2.0052631354423127 1208 60-61 -1.1309930141330469 1208 62-63 0.5081406383809508 1208 64-65 0.05521226040455218 1208 66-67 0.9513381747252403 1208 68-69 0.539035493230017 1208 70-71 -4.324796666910306 1208 72-73 -0.7861353204334094 1208 74-75 -5.766468664287707 1208 76-77 -1.7048602337125907 1208 78-79 -4.493423019449658 1208 80-81 0.7074483526093012 1208 82-83 0.4151405961680119 1208 84-85 -3.1597428180325196 1208 86-87 -0.9720662978352088 1208 88-89 -5.209063820932357 1208 90-91 -0.26429440090838696 1208 92-93 -1.9272234954494927 1208 94-95 -4.88713066851026 1208 96-97 -3.7055674040361204 1208 98-99 -6.513471373495101 1208 100-101 -4.09314043248169 1209 1 1.8030960258057256 1209 2 1.4213770164543966 1209 3 0.5681761010516304 1209 4 0.15736917487583924 1209 5 0.3212947155405814 1209 6 0.8946877415957388 1209 7 0.6369215659609182 1209 8 0.2613352673888869 1209 9 -0.3700121332357611 1209 10-11 -2.9544411973901816 1209 12-13 -0.4167161641899142 1209 14-15 -1.2588314914846137 1209 16-17 -0.2042519975562982 1209 18-19 -16.548980144254276 1209 20-21 -14.243289420698833 1209 22-23 -3.2308893140725523 1209 24-25 -2.234765391462304 1209 26-27 -1.1823519495322898 1209 28-29 -2.7979606644018347 1209 30-31 -0.6443007282026585 1209 32-33 -0.40116481231524403 1209 34-35 -3.2337660320636346 1209 36-37 -3.864062868784483 1209 38-39 -1.4630447914645828 1209 40-41 -9.688985196329924 1209 42-43 -4.9387550778055385 1209 44-45 -3.1370953664164567 1209 46-47 -2.1062135268193174 1209 48-49 -3.1802204882153724 1209 50-51 -2.294978970542278 1209 52-53 -0.9759631908364845 1209 54-55 -3.0989085357886346 1209 56-57 -1.163551715034174 1209 58-59 -2.3134804259429202 1209 60-61 -1.9771310636177866 1209 62-63 -0.8851894471754527 1209 64-65 -0.1469200710962788 1209 66-67 0.19464585773272702 1209 68-69 -1.5631432435507016 1209 70-71 -1.7322821408131155 1209 72-73 -0.8719593075181677 1209 74-75 0.6283967070612135 1209 76-77 -15.125995260631207 1209 78-79 -14.9578100785995 1209 80-81 -20.350572471093308 1209 82-83 -9.346947001354192 1209 84-85 -3.592240026603804 1209 86-87 -2.4159621366506485 1209 88-89 -2.205930131054753 1209 90-91 -6.045473059457986 1209 92-93 -3.073555700358142 1209 94-95 -0.8190927050797541 1209 96-97 0.09087965838520518 1209 98-99 1.074624872122179 1209 100-101 1.0659683372272184 1212 1 -28.624344607439657 1212 2 -16.280469949244814 1212 3 -5.593704750688119 1212 4 -1.5392007459684862 1212 5 -0.33631508317280634 1212 6 -2.2235074737659275 1212 7 -0.20596721899509163 1212 8 -1.1393274517893772 1212 9 0.043551989918817924 1212 10-11 0.8240905840095607 1212 12-13 0.21332813474850099 1212 14-15 -0.004134656829080541 1212 16-17 0.3655122120782792 1212 18-19 -1.0563883033774744 1212 20-21 -0.6366101644268198 1212 22-23 -0.560338401179088 1212 24-25 0.580705703921609 1212 26-27 -0.7171447063911245 1212 28-29 0.15820147677235497 1212 30-31 -0.6303867140305286 1212 32-33 0.5715829572949787 1212 34-35 -1.0935868066773438 1212 36-37 -1.2264074474592235 1212 38-39 -0.4046090205130355 1212 40-41 -0.42275475360526826 1212 42-43 0.23050966571066667 1212 44-45 1.4678013429313275 1212 46-47 -1.7238858911648514 1212 48-49 -1.1263888947772642 1212 50-51 0.05943680411555263 1212 52-53 -0.9037501853035295 1212 54-55 -0.5007868284263601 1212 56-57 -1.5968143053748918 1212 58-59 -0.5518210688952436 1212 60-61 0.3946821925306736 1212 62-63 -3.843367984251543 1212 64-65 -3.576983783912084 1212 66-67 -1.6796735534973166 1212 68-69 0.5040243599826262 1212 70-71 -0.40612458837358645 1212 72-73 -1.357220203556853 1212 74-75 0.22137153079202676 1212 76-77 0.8377537355830889 1212 78-79 -0.9481913848004702 1212 80-81 0.8802210270226638 1212 82-83 1.1433372354598141 1212 84-85 -2.8779770038630943 1212 86-87 -1.5820037352952063 1212 88-89 -0.32508059742903583 1212 90-91 0.2795196603379928 1212 92-93 -2.1650524220602065 1212 94-95 -1.7552828198863644 1212 96-97 0.39834252934469205 1212 98-99 -0.1288435935637935 1212 100-101 0.5156555090665051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 2.0 11 11.0 12 68.0 13 294.0 14 998.0 15 2282.0 16 4130.0 17 6772.0 18 10146.0 19 14351.0 20 19040.0 21 24623.0 22 31059.0 23 38492.0 24 45881.0 25 53885.0 26 63393.0 27 73028.0 28 83985.0 29 94716.0 30 107252.0 31 120488.0 32 132589.0 33 144137.0 34 153061.0 35 157223.0 36 148758.0 37 116401.0 38 37809.0 39 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.430461189204316 18.923658742582937 16.978172756621184 25.667707311591563 2 26.785307314293856 25.652256486954872 23.37659948246801 24.185836716283266 3 22.6825672653724 23.003641612184918 27.699339097241015 26.61445202520167 4 26.099946052419952 23.406068598294688 25.192985746325302 25.300999602960054 5 24.4417553082579 25.01879856543355 24.50371490514069 26.035731221167868 6 23.898125128711566 25.660946877954434 24.519026759542754 25.921901233791246 7 25.598868584525885 23.905306269729593 25.404621687401963 25.09120345834256 8 25.079096410675806 24.208219854489904 25.119631281050275 25.593052453784015 9 24.681225589313513 23.809933595217476 25.81738892525619 25.691451890212818 10-11 25.615130011293974 23.841210135023257 24.56831550016647 25.975344353516295 12-13 25.687602084167 24.019679835511823 24.304370911802252 25.98834716851892 14-15 25.553170414411184 24.258161426115258 24.310862692429357 25.8778054670442 16-17 25.709533594961314 24.030712849692502 24.21590984962573 26.043843705720455 18-19 25.840521620712725 24.23232180395018 23.995135057413215 25.932021517923882 20-21 25.525640390309967 24.249830189703843 24.370069826231173 25.85445959375502 22-23 25.8191100251696 23.9853374157032 24.13082970665929 26.06472285246791 24-25 25.665783762193843 23.950885743298542 24.354453998856954 26.02887649565066 26-27 25.743529999661714 24.117357648716386 24.313830106073166 25.82528224554873 28-29 25.931575002270073 24.030501451362014 24.11050292319918 25.927420623168736 30-31 25.80531155172588 24.051421717550888 24.12691272064946 26.016354010073776 32-33 25.737713868919844 24.08714937782238 24.228071851767005 25.94706490149077 34-35 26.080895256826686 23.954802729308373 24.012103486770382 25.95219852709456 36-37 25.857953469767097 23.961390387597636 24.249051466228757 25.93160467640651 38-39 25.804480675905612 24.102372209815137 24.207329630396764 25.885817483882494 40-41 26.05480330101755 23.898963461498514 24.027722137649228 26.01851109983471 42-43 25.71127421335348 23.969135337207984 24.25575782106377 26.063832628374765 44-45 25.721274397333126 24.038958580246877 24.382911495701105 25.856855526718892 46-47 26.00355043671084 23.8565483474679 24.136677269695767 26.00322394612549 48-49 25.83992693036307 23.941137161061814 24.243279397590932 25.975656510984184 50-51 25.778167366333555 24.090658074616826 24.270305349921973 25.860869209127646 52-53 26.008076122559075 23.961122087257493 24.12625885254726 25.904542937636165 54-55 25.6803691071029 23.935371834217545 24.403966690624127 25.98029236805543 56-57 25.772319098318842 24.10163795004159 24.245767583028847 25.88027536861072 58-59 25.970647195158936 23.95047727787799 24.175237629047107 25.90363789791597 60-61 25.91406155180145 23.946037199577315 24.162929388817457 25.976971859803783 62-63 25.913702202060662 24.107203531036188 24.123435519686257 25.855658747216896 64-65 26.03863024048273 23.969100080804857 23.9685659319251 26.02370374678732 66-67 25.902416355222556 24.024908406646595 24.098681637350264 25.97399360078058 68-69 25.924238813712382 24.105837396168823 24.062243955305 25.907679834813802 70-71 26.208457264542574 23.987354787936304 23.780280982950686 26.023906964570436 72-73 25.922006384685304 24.028003797326317 24.041184010620164 26.008805807368212 74-75 25.83454777065526 24.082017490866114 24.162082778066566 25.921351960412064 76-77 25.995792859868587 24.07779345664292 23.927440801056598 25.998972882431893 78-79 25.95261735717639 24.15296359623327 23.841591773825304 26.05282727276504 80-81 25.75318210918619 24.212609010170567 24.02717103103457 26.007037849608672 82-83 26.05495853286434 24.137086816130562 23.687502170024903 26.120452480980195 84-85 25.844495842733107 24.07454888505479 23.930117319858198 26.150837952353907 86-87 25.811374978039662 24.254149909547166 23.859012283542334 26.075462828870837 88-89 25.993118112362616 24.136729664081383 23.651822186742795 26.218330036813203 90-91 25.92989105673924 24.101496355943645 23.63832796358763 26.330284623729476 92-93 25.780275286534227 24.241033007081 23.77325957537735 26.205432131007427 94-95 26.035317336657315 24.071821810681268 23.53986555669323 26.352995295968185 96-97 25.899092264258623 24.14270803625245 23.565249571684177 26.392950127804752 98-99 25.779889237402394 24.219086886702048 23.645419609567927 26.35560426632763 100-101 26.022512579040058 23.91433253776383 23.412803228021776 26.650351655174337 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 4.5 5 8.0 6 17.0 7 26.5 8 34.0 9 51.5 10 77.5 11 119.0 12 193.5 13 296.0 14 435.0 15 619.5 16 894.5 17 1219.0 18 1636.5 19 2255.5 20 2971.5 21 3857.0 22 4979.0 23 6350.0 24 7882.0 25 9578.0 26 11489.0 27 13468.5 28 15728.0 29 18057.5 30 20413.5 31 22764.5 32 25022.0 33 27107.0 34 28889.5 35 30740.0 36 32414.5 37 33834.0 38 34863.0 39 35355.0 40 35390.5 41 34986.0 42 34422.5 43 33791.5 44 33111.5 45 32512.5 46 32198.5 47 32041.5 48 31636.5 49 30962.5 50 30415.0 51 30295.0 52 30324.5 53 30481.0 54 30999.5 55 31815.0 56 32718.0 57 34021.5 58 35627.0 59 36939.0 60 38249.5 61 39534.0 62 40523.0 63 41635.0 64 42696.0 65 43210.0 66 43330.0 67 43049.5 68 42054.0 69 40315.0 70 38031.5 71 35123.0 72 31521.0 73 27194.5 74 22696.0 75 18438.0 76 14327.5 77 10685.0 78 7732.0 79 5425.5 80 3686.0 81 2403.0 82 1534.5 83 1026.0 84 689.0 85 456.5 86 325.0 87 231.0 88 160.5 89 117.5 90 85.5 91 53.0 92 35.0 93 27.5 94 15.5 95 6.0 96 4.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7102801297828031 2 0.0029080653709356074 3 5.9348272876236894E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.18670966646864126 14-15 0.0 16-17 3.857637736955398E-4 18-19 0.11317715637498375 20-21 2.9674136438118447E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0020178412777920544 42-43 0.0 44-45 0.0 46-47 0.022997455739541796 48-49 5.9348272876236894E-5 50-51 2.9674136438118447E-5 52-53 1.1869654575247379E-4 54-55 0.002255234369297002 56-57 0.0016320775040965145 58-59 0.09617387619594188 60-61 0.0018101223227252254 62-63 0.0014540326854678037 64-65 0.0027003464158687784 66-67 0.004510468738594004 68-69 0.004896232512289544 70-71 0.0037092670547648056 72-73 0.037152018820524296 74-75 0.0426417340615762 76-77 0.15377137502232976 78-79 3.5608963725742134E-4 80-81 0.11433444769607037 82-83 0.004777535966537069 84-85 0.005222648013108846 86-87 0.006706354835014769 88-89 0.006617332425700413 90-91 0.02234462473790319 92-93 0.010623340844846405 94-95 0.014480978581801803 96-97 0.010237577071150862 98-99 0.011543239074428075 100-101 0.01317531657852459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1684969.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.10478580697448 #Duplication Level Percentage of deduplicated Percentage of total 1 99.33738373554442 98.4481013773636 2 0.5182843194182046 1.0272891292610942 3 0.0929217028642332 0.2762695637753747 4 0.04127905975001838 0.16363809499355492 5 0.0050925618092996245 0.025234862365970882 6 0.0021269345678214383 0.012647363686163609 7 0.0018274082576789938 0.012677343277142115 8 0.0 0.0 9 0.0 0.0 >10 9.980275284350794E-4 0.012858209576552948 >50 0.0 0.0 >100 8.625025991183253E-5 0.02128405570055152 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.7804481862871067E-4 0.0 0.0 2 2.3739309150494758E-4 0.0 0.0 3 3.5608963725742134E-4 0.0 0.0 4 4.7478618300989515E-4 5.9348272876236894E-5 0.0 5 7.715275473910796E-4 5.9348272876236894E-5 5.9348272876236894E-5 6 0.0010089206388960272 5.9348272876236894E-5 5.9348272876236894E-5 7 0.0014243585490296853 1.1869654575247379E-4 5.9348272876236894E-5 8 0.001602403367658396 1.1869654575247379E-4 5.9348272876236894E-5 9 0.0018397964591633437 1.1869654575247379E-4 5.9348272876236894E-5 10-11 0.0021068636871064096 1.1869654575247379E-4 5.9348272876236894E-5 12-13 0.0025816498701163046 1.7804481862871067E-4 5.9348272876236894E-5 14-15 0.0029674136438118445 1.7804481862871067E-4 5.9348272876236894E-5 16-17 0.003175132598878674 1.7804481862871067E-4 5.9348272876236894E-5 18-19 0.0035015480996979766 1.7804481862871067E-4 5.9348272876236894E-5 20-21 0.0038279636005172793 1.7804481862871067E-4 5.9348272876236894E-5 22-23 0.004095030828460345 1.7804481862871067E-4 8.902240931435535E-5 24-25 0.004629165284346478 1.7804481862871067E-4 1.1869654575247379E-4 26-27 0.005638085923242504 1.7804481862871067E-4 1.1869654575247379E-4 28-29 0.006914073790081598 1.7804481862871067E-4 1.1869654575247379E-4 30-31 0.009673768478826613 1.7804481862871067E-4 1.1869654575247379E-4 32-33 0.014866742355497342 1.7804481862871067E-4 1.1869654575247379E-4 34-35 0.020860917915997267 1.7804481862871067E-4 1.1869654575247379E-4 36-37 0.0300895743482521 1.7804481862871067E-4 1.1869654575247379E-4 38-39 0.04207792546925196 2.0771895506682914E-4 1.1869654575247379E-4 40-41 0.05842837464665522 2.3739309150494758E-4 1.1869654575247379E-4 42-43 0.08006082011004356 2.3739309150494758E-4 1.1869654575247379E-4 44-45 0.10736102563311253 2.3739309150494758E-4 1.1869654575247379E-4 46-47 0.1398245308964141 2.3739309150494758E-4 1.4837068219059223E-4 48-49 0.1840389941892106 2.3739309150494758E-4 1.7804481862871067E-4 50-51 0.23739309150494758 2.3739309150494758E-4 1.7804481862871067E-4 52-53 0.3014892262112834 2.3739309150494758E-4 1.7804481862871067E-4 54-55 0.37297422089071075 2.3739309150494758E-4 1.7804481862871067E-4 56-57 0.4565959373733285 2.3739309150494758E-4 1.7804481862871067E-4 58-59 0.5544018910733669 2.67067227943066E-4 1.7804481862871067E-4 60-61 0.6677867664034175 2.9674136438118446E-4 1.7804481862871067E-4 62-63 0.7893617033903888 2.9674136438118446E-4 1.7804481862871067E-4 64-65 0.9268716516446296 2.9674136438118446E-4 1.7804481862871067E-4 66-67 1.076993107885071 2.9674136438118446E-4 1.7804481862871067E-4 68-69 1.2499339750464253 3.264155008193029E-4 1.7804481862871067E-4 70-71 1.4323408917315392 3.5608963725742134E-4 1.7804481862871067E-4 72-73 1.6311576058669328 3.5608963725742134E-4 1.7804481862871067E-4 74-75 1.8460577019517865 3.5608963725742134E-4 2.0771895506682914E-4 76-77 2.0852015675065836 3.8576377369553983E-4 2.3739309150494758E-4 78-79 2.3480550680754364 4.1543791013365827E-4 2.3739309150494758E-4 80-81 2.6281789160512745 4.1543791013365827E-4 2.3739309150494758E-4 82-83 2.930172602582006 4.451120465717767E-4 2.9674136438118446E-4 84-85 3.244570078143871 4.7478618300989515E-4 2.9674136438118446E-4 86-87 3.5775435631159977 4.7478618300989515E-4 2.9674136438118446E-4 88-89 3.9339596158742385 4.7478618300989515E-4 2.9674136438118446E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 960 1.4551915E-11 6.920982 94-95 CGTATGC 1000 4.1836756E-11 6.6441426 94-95 TCGTATG 925 1.4826583E-8 6.1567345 94-95 CCAGTCA 3055 0.0 5.592451 94-95 TCTCGTA 1190 2.9831426E-9 5.583313 94-95 CAGTCAC 2990 0.0 5.39658 94-95 >>END_MODULE