##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib13_AGTCAA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1229878 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.839934530091604 27.0 22.0 33.0 2.0 33.0 2 24.874583495273516 33.0 15.0 33.0 2.0 33.0 3 25.744942181257002 33.0 22.0 33.0 6.0 33.0 4 27.837613974719442 33.0 22.0 37.0 2.0 37.0 5 27.87957992581378 33.0 15.0 37.0 6.0 37.0 6 28.947363071784356 33.0 22.0 37.0 15.0 37.0 7 28.615975730926156 33.0 27.0 37.0 6.0 37.0 8 28.379258755746505 33.0 22.0 37.0 2.0 37.0 9 29.141069276790056 33.0 27.0 37.0 15.0 37.0 10-11 28.754104878695287 33.0 24.5 37.0 10.5 37.0 12-13 29.027159197904183 33.0 27.0 37.0 10.5 37.0 14-15 29.32577052358039 33.0 27.0 37.0 6.0 40.0 16-17 30.191462893067442 33.0 27.0 37.0 15.0 40.0 18-19 30.47044178365659 33.0 27.0 37.0 15.0 40.0 20-21 30.487123113024218 33.0 27.0 37.0 15.0 40.0 22-23 30.499153981126582 33.0 27.0 37.0 15.0 40.0 24-25 30.034113139677267 33.0 27.0 37.0 10.5 40.0 26-27 29.49496738700912 33.0 27.0 37.0 10.5 40.0 28-29 29.66554528172713 33.0 27.0 37.0 15.0 40.0 30-31 29.524016609777554 33.0 27.0 37.0 10.5 40.0 32-33 29.342001401765053 33.0 27.0 37.0 10.5 40.0 34-35 29.347568214083026 33.0 24.5 37.0 10.5 40.0 36-37 29.57582662670606 33.0 27.0 37.0 6.0 40.0 38-39 29.616941273849925 33.0 27.0 37.0 15.0 40.0 40-41 29.66000692751639 33.0 27.0 37.0 15.0 40.0 42-43 29.532359307183313 33.0 27.0 37.0 15.0 40.0 44-45 29.234326087628205 33.0 24.5 37.0 10.5 40.0 46-47 28.82317392456813 33.0 22.0 37.0 10.5 40.0 48-49 28.628428592104257 33.0 22.0 37.0 10.5 40.0 50-51 27.815491455250033 33.0 22.0 37.0 10.5 38.5 52-53 27.508212603201294 33.0 22.0 37.0 6.0 37.0 54-55 28.393179242168735 33.0 22.0 37.0 6.0 40.0 56-57 28.285421399520928 33.0 22.0 37.0 6.0 40.0 58-59 28.455002040852833 33.0 22.0 37.0 10.5 40.0 60-61 28.17801481122518 33.0 22.0 37.0 10.5 40.0 62-63 28.01847459666731 33.0 22.0 37.0 10.5 40.0 64-65 27.978766186564847 33.0 22.0 37.0 6.0 40.0 66-67 27.81006246147992 33.0 22.0 37.0 10.5 37.0 68-69 27.4868182047325 33.0 22.0 37.0 6.0 37.0 70-71 27.36018247338354 33.0 22.0 37.0 6.0 37.0 72-73 27.083429413323923 27.0 22.0 35.0 6.0 37.0 74-75 26.998533594389038 27.0 22.0 33.0 6.0 37.0 76-77 26.58485801030671 27.0 22.0 33.0 6.0 37.0 78-79 25.95439994861279 27.0 22.0 33.0 6.0 37.0 80-81 25.82075132655434 27.0 22.0 33.0 6.0 37.0 82-83 25.36573790245862 27.0 18.5 33.0 6.0 37.0 84-85 25.411879877516306 27.0 18.5 33.0 6.0 37.0 86-87 25.080628322484017 27.0 15.0 33.0 6.0 37.0 88-89 24.667235693296405 27.0 15.0 33.0 6.0 37.0 90-91 24.477253028349153 27.0 15.0 33.0 6.0 37.0 92-93 24.308652158994633 27.0 15.0 33.0 6.0 37.0 94-95 24.037087011882477 27.0 15.0 33.0 6.0 37.0 96-97 23.84626930476031 27.0 15.0 33.0 6.0 37.0 98-99 23.552780844929334 27.0 15.0 33.0 6.0 37.0 100-101 23.001192394692808 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.228267373310842 2306 2 8.527738389408224 2306 3 7.48787215714006 2306 4 9.156643908845318 2306 5 9.045194706264343 2306 6 7.9792348920291545 2306 7 8.2294720521476 2306 8 8.610699061428662 2306 9 7.576850449855645 2306 10-11 7.833116129823956 2306 12-13 7.423572129307729 2306 14-15 7.98599667022831 2306 16-17 6.694218585694138 2306 18-19 6.138840355667295 2306 20-21 6.0282056555119325 2306 22-23 5.624956720853255 2306 24-25 5.635543578385377 2306 26-27 6.260117089964915 2306 28-29 5.395412089974492 2306 30-31 5.154844607815569 2306 32-33 4.981983878074988 2306 34-35 4.400841066121494 2306 36-37 4.53494636393156 2306 38-39 4.196770125526076 2306 40-41 3.3712631528051844 2306 42-43 3.345847218063973 2306 44-45 2.9072901380776592 2306 46-47 3.234209005949978 2306 48-49 3.016686425281847 2306 50-51 2.8834293439335106 2306 52-53 3.4256752002178423 2306 54-55 3.017363185342898 2306 56-57 3.3784798193267207 2306 58-59 2.8542694281937244 2306 60-61 2.913205196471438 2306 62-63 2.457572427430687 2306 64-65 2.0100889223059752 2306 66-67 1.8215107247803708 2306 68-69 2.0269520973234307 2306 70-71 2.115013549813 2306 72-73 1.2327010400229703 2306 74-75 0.8275677543328932 2306 76-77 1.2662180936761231 2306 78-79 1.570056966335006 2306 80-81 1.2858971621337147 2306 82-83 1.0989930824119334 2306 84-85 0.6672295802015569 2306 86-87 0.6673158949402378 2306 88-89 0.7240122041694868 2306 90-91 0.3432335585413391 2306 92-93 0.5559825821644679 2306 94-95 0.660249520168712 2306 96-97 0.33371214660502346 2306 98-99 0.23484795033430927 2306 100-101 0.09803999859276402 2307 1 8.516292502937912 2307 2 8.590672002100572 2307 3 7.792886421775169 2307 4 9.360653198839373 2307 5 9.283696407166953 2307 6 7.785226485602152 2307 7 8.32656353102984 2307 8 8.670179843989445 2307 9 7.657492591218588 2307 10-11 7.85135242044516 2307 12-13 7.574280200916149 2307 14-15 8.268034516014005 2307 16-17 7.079597237416948 2307 18-19 6.488870271546812 2307 20-21 6.44005650939372 2307 22-23 6.256607923174684 2307 24-25 6.417451195324329 2307 26-27 7.035788064008948 2307 28-29 6.238775542902637 2307 30-31 5.863434393002116 2307 32-33 5.909372395479739 2307 34-35 5.709778267566282 2307 36-37 5.477035606509922 2307 38-39 5.064683337620938 2307 40-41 4.485965795016597 2307 42-43 4.2087069455246215 2307 44-45 4.172032050736551 2307 46-47 4.375819507973176 2307 48-49 3.942054774007456 2307 50-51 3.869552656742048 2307 52-53 4.582056622708905 2307 54-55 4.044165463936924 2307 56-57 4.379121090095051 2307 58-59 4.011717905640495 2307 60-61 4.061295790777663 2307 62-63 3.521610426867685 2307 64-65 3.4166385900890894 2307 66-67 3.270615028501428 2307 68-69 3.2456937508218715 2307 70-71 2.8481886962970293 2307 72-73 2.205452942034377 2307 74-75 1.8497637369999858 2307 76-77 1.906044309153767 2307 78-79 2.496974151887116 2307 80-81 2.23586139424404 2307 82-83 2.3344634132249134 2307 84-85 1.7485712962271442 2307 86-87 1.4495372286306498 2307 88-89 1.6264030530093265 2307 90-91 1.372999390210719 2307 92-93 1.3649913482162361 2307 94-95 1.364002100061981 2307 96-97 1.2829059601366275 2307 98-99 0.8850761138683048 2307 100-101 0.5824745884699141 1101 1 8.001979192739952 1101 2 7.796331593445174 1101 3 7.352990302092341 1101 4 8.605335698722769 1101 5 8.583750696785554 1101 6 7.331266776378968 1101 7 6.904201886268773 1101 8 7.9711582738606666 1101 9 7.315909495510976 1101 10-11 7.610697245563927 1101 12-13 7.273895943978509 1101 14-15 7.757204186136498 1101 16-17 6.996055610402848 1101 18-19 6.05501886924365 1101 20-21 5.858874047316071 1101 22-23 5.23449692610847 1101 24-25 5.907920166084121 1101 26-27 6.457352022191451 1101 28-29 5.769834187568392 1101 30-31 5.545166139131815 1101 32-33 5.731924140815661 1101 34-35 5.56413347405368 1101 36-37 5.279767150719316 1101 38-39 4.958691258574792 1101 40-41 4.618251692143851 1101 42-43 4.312543373279301 1101 44-45 4.285401101076701 1101 46-47 4.674729341144133 1101 48-49 4.444531991342938 1101 50-51 3.818723421927281 1101 52-53 4.599341811521764 1101 54-55 4.431894083633512 1101 56-57 4.573180041552195 1101 58-59 4.083516288366084 1101 60-61 4.280355053661381 1101 62-63 3.989813049398105 1101 64-65 3.6846459988548617 1101 66-67 3.3894876382300474 1101 68-69 3.340604692961133 1101 70-71 3.352988504052682 1101 72-73 2.894447952546109 1101 74-75 2.101519096506479 1101 76-77 2.5302622301163105 1101 78-79 3.051349666284146 1101 80-81 2.4309913634171565 1101 82-83 2.562388628551041 1101 84-85 1.8270049480267367 1101 86-87 2.029730875122958 1101 88-89 2.1459306580057103 1101 90-91 1.9821888062265067 1101 92-93 1.6520175054411723 1101 94-95 1.5556624874998164 1101 96-97 1.5171474133602132 1101 98-99 1.507009684696989 1101 100-101 1.3111575118934482 2201 1 7.146901214367187 2201 2 7.870268357175586 2201 3 6.4212537299869865 2201 4 8.390295702562128 2201 5 3.607817810120295 2201 6 5.524648684436649 2201 7 6.631809778204584 2201 8 7.890320951195264 2201 9 6.530097634296876 2201 10-11 4.022236484994124 2201 12-13 6.5040032745797305 2201 14-15 7.041852242204818 2201 16-17 5.9832031908365195 2201 18-19 1.8441318920346852 2201 20-21 5.016404243688903 2201 22-23 4.928429027762306 2201 24-25 5.43590419504385 2201 26-27 6.194750274941136 2201 28-29 5.158189023015943 2201 30-31 5.200449283153986 2201 32-33 5.156557902134484 2201 34-35 4.788705445717856 2201 36-37 4.815928667676765 2201 38-39 4.499878219843556 2201 40-41 4.016058373653749 2201 42-43 3.968074618619365 2201 44-45 3.7233204951438665 2201 46-47 4.183284765864453 2201 48-49 3.9753338342343874 2201 50-51 3.736291223432513 2201 52-53 4.47687574441186 2201 54-55 4.274652696048687 2201 56-57 4.279207832327796 2201 58-59 3.893998590472073 2201 60-61 3.844868680808375 2201 62-63 3.619459914237307 2201 64-65 3.579252644713584 2201 66-67 3.191798879833595 2201 68-69 3.50463137306555 2201 70-71 3.2712881830207188 2201 72-73 2.5497568065494107 2201 74-75 2.231472767120483 2201 76-77 2.6453662441299635 2201 78-79 2.9578420364187465 2201 80-81 2.2404332682867967 2201 82-83 2.570272165764525 2201 84-85 2.2127429358950543 2201 86-87 2.066542854602968 2201 88-89 2.0640259426237684 2201 90-91 1.8426521686463957 2201 92-93 1.5206750673465237 2201 94-95 1.8460400128627157 2201 96-97 1.8029674160683307 2201 98-99 1.6474307456279327 2201 100-101 1.2748395925508014 2304 1 1.432519165162823 2304 2 4.819002451776893 2304 3 5.663645479626943 2304 4 3.9682161111172256 2304 5 6.351037575173631 2304 6 6.093219388817431 2304 7 6.79339933638785 2304 8 6.144926470550757 2304 9 5.649771895202729 2304 10-11 6.520069181768008 2304 12-13 6.3560623211181735 2304 14-15 6.571000102255034 2304 16-17 5.296440788955444 2304 18-19 5.141381289915618 2304 20-21 4.92071250912678 2304 22-23 4.50304261755624 2304 24-25 4.727788486940614 2304 26-27 5.544319038005426 2304 28-29 4.862714705776121 2304 30-31 4.895225084421604 2304 32-33 5.036282516501661 2304 34-35 4.777232098955491 2304 36-37 4.766384690838052 2304 38-39 4.350266953193309 2304 40-41 3.878799542182364 2304 42-43 3.401946830892147 2304 44-45 3.1224219357098946 2304 46-47 4.1256664636142375 2304 48-49 3.813899122164994 2304 50-51 3.2377916849878297 2304 52-53 3.9151893192725886 2304 54-55 3.7865718694506647 2304 56-57 4.455630527326331 2304 58-59 3.6123112722332102 2304 60-61 3.75553919611961 2304 62-63 3.285252825774524 2304 64-65 3.2138877701398414 2304 66-67 3.102587631406916 2304 68-69 3.43496933352257 2304 70-71 3.216278942874915 2304 72-73 2.8415397659922057 2304 74-75 2.2052047916832542 2304 76-77 2.552789510784553 2304 78-79 2.989819431236384 2304 80-81 2.664000123661431 2304 82-83 2.7328033654937762 2304 84-85 2.2899386501740544 2304 86-87 2.09941949585426 2304 88-89 2.2893134791115877 2304 90-91 2.422026937461961 2304 92-93 1.9739247385810117 2304 94-95 2.1971234294495616 2304 96-97 2.1838162692669805 2304 98-99 2.0171156731078845 2304 100-101 1.950809229334066 1102 1 6.968864313385044 1102 2 7.466112158186338 1102 3 6.244929893327125 1102 4 6.2194971946133 1102 5 6.733238287399178 1102 6 5.813662320032137 1102 7 6.77546266010841 1102 8 6.412297645212426 1102 9 4.927668283448654 1102 10-11 6.038390931739016 1102 12-13 5.880485861179395 1102 14-15 6.054613367681945 1102 16-17 5.031088035527816 1102 18-19 5.281887565350626 1102 20-21 5.607204441571675 1102 22-23 5.298215369130858 1102 24-25 1.7424870069995144 1102 26-27 5.41331349121586 1102 28-29 4.965331920620109 1102 30-31 5.036270784589483 1102 32-33 5.0730053022324775 1102 34-35 5.034731740712349 1102 36-37 4.959555822932593 1102 38-39 3.934069218254642 1102 40-41 3.6857714058767286 1102 42-43 4.094587180304313 1102 44-45 -3.541693718239088 1102 46-47 1.5310151090532145 1102 48-49 -0.7004426620888289 1102 50-51 2.775252461634839 1102 52-53 -0.296951616090702 1102 54-55 2.714137719363169 1102 56-57 -0.7148464796944332 1102 58-59 2.7341740320499284 1102 60-61 3.7392553622407227 1102 62-63 3.4249494616654452 1102 64-65 3.0633828432666625 1102 66-67 2.979590853685881 1102 68-69 3.2708056458511017 1102 70-71 3.1284441563707404 1102 72-73 2.6947571511448807 1102 74-75 2.30160455364501 1102 76-77 2.651750937293663 1102 78-79 -1.530702524495819 1102 80-81 1.4698755523222076 1102 82-83 2.4485079408163806 1102 84-85 2.027254983991142 1102 86-87 1.976194407217971 1102 88-89 2.132830564545891 1102 90-91 1.8807646634892627 1102 92-93 1.883419859942535 1102 94-95 2.280767022444355 1102 96-97 2.2887401076799314 1102 98-99 2.1298466997372536 1102 100-101 1.5411436786977788 2202 1 -20.614573578710313 2202 2 -7.66204682341132 2202 3 -0.8779808919091785 2202 4 1.4287186239315766 2202 5 1.1340793319412121 2202 6 1.4863339271003078 2202 7 4.002921751492767 2202 8 2.7095706220003812 2202 9 3.301829199383409 2202 10-11 1.9144492624583833 2202 12-13 1.3762515580635508 2202 14-15 2.4741638957036898 2202 16-17 1.3559834767657932 2202 18-19 1.364650726684804 2202 20-21 0.7084682956076378 2202 22-23 1.090081350937627 2202 24-25 2.2634013733734193 2202 26-27 3.3580626808854532 2202 28-29 -1.5729927866153588 2202 30-31 0.511784756254059 2202 32-33 1.5226132068748832 2202 34-35 1.5641251691941562 2202 36-37 2.7307489037565205 2202 38-39 2.2915013857577797 2202 40-41 1.5157062526981449 2202 42-43 0.7003892113582673 2202 44-45 0.7427864940151991 2202 46-47 -1.3420802877472866 2202 48-49 0.5972423415707304 2202 50-51 1.3815449386319116 2202 52-53 1.6825790457662642 2202 54-55 -0.8435270913854716 2202 56-57 0.9509141199500668 2202 58-59 1.7720354132711726 2202 60-61 2.0033642601913684 2202 62-63 -2.203098752993693 2202 64-65 -0.10928255562501477 2202 66-67 0.24415556248972692 2202 68-69 1.3661949311919592 2202 70-71 -2.482040800237371 2202 72-73 -4.486086049921621 2202 74-75 -1.1798097996590506 2202 76-77 -0.3524369678715402 2202 78-79 1.4206400753577988 2202 80-81 1.5662656610633192 2202 82-83 -0.8293533750556428 2202 84-85 -0.4884503955571624 2202 86-87 0.6178555192004076 2202 88-89 1.0234209294559733 2202 90-91 1.16079786089621 2202 92-93 1.4050612364586357 2202 94-95 1.5626296714405896 2202 96-97 1.0818689632842826 2202 98-99 1.1076005161320701 2202 100-101 0.9597812780286077 2305 1 5.7422910756226955 2305 2 7.2984438857740095 2305 3 7.01418733675035 2305 4 8.007685984366503 2305 5 8.103654449597816 2305 6 6.561604233007479 2305 7 3.14443576885726 2305 8 6.348268995093534 2305 9 6.781971604446699 2305 10-11 5.180006052055916 2305 12-13 5.5896078648632965 2305 14-15 6.022279868602105 2305 16-17 5.283985748755406 2305 18-19 4.568078743156729 2305 20-21 5.4442592319919285 2305 22-23 5.446248687029808 2305 24-25 5.270884739163744 2305 26-27 6.063108222105832 2305 28-29 5.238648117311939 2305 30-31 5.470865868311833 2305 32-33 5.515752609666183 2305 34-35 5.464264632842319 2305 36-37 4.6391058191249 2305 38-39 4.627275586504293 2305 40-41 4.163758579458936 2305 42-43 4.095422530491671 2305 44-45 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-25.198220126458448 1209 34-35 -22.41806544025858 1209 36-37 -25.569511512910147 1209 38-39 -25.834019447575553 1209 40-41 -22.75034241023718 1209 42-43 -22.847010508790987 1209 44-45 -25.819192672651468 1209 46-47 -19.21917528152838 1209 48-49 -19.050569381066087 1209 50-51 -21.240368929527293 1209 52-53 -23.838775120900404 1209 54-55 -21.019903024221293 1209 56-57 -24.60924213004993 1209 58-59 -24.863902086728828 1209 60-61 -24.553927406475296 1209 62-63 -24.65179666966036 1209 64-65 -24.550167972291682 1209 66-67 -12.208833398953058 1209 68-69 -24.13927381880804 1209 70-71 -23.986988716904037 1209 72-73 -14.177484526289778 1209 74-75 -15.347529793440144 1209 76-77 -12.328622446727262 1209 78-79 -12.82856312737852 1209 80-81 -22.881651005603345 1209 82-83 -14.830678779284497 1209 84-85 -11.703134786104428 1209 86-87 -11.86629015866029 1209 88-89 -9.151073784474374 1209 90-91 -12.32659330825802 1209 92-93 -15.903174926476687 1209 94-95 -21.264193245226075 1209 96-97 -16.866533555372435 1209 98-99 -8.812004583370419 1209 100-101 -4.10781822445896 1212 1 -20.614573578710313 1212 2 -20.431838490077986 1212 3 -21.336835058575844 1212 4 -23.293677209401757 1212 5 -23.459149834725455 1212 6 -24.501686906233026 1212 7 -24.096557415173898 1212 8 -23.731575211332952 1212 9 -24.62942080061659 1212 10-11 -24.33216532087495 1212 12-13 -24.595883858603116 1212 14-15 -24.92635693762964 1212 16-17 -25.885683189900874 1212 18-19 -20.65528116434084 1212 20-21 -26.277990037725694 1212 22-23 -26.454189482395705 1212 24-25 -22.624258883036838 1212 26-27 -25.125791485781214 1212 28-29 -20.118505607128178 1212 30-31 -14.752417968104915 1212 32-33 -25.198220126458448 1212 34-35 -21.264039894908407 1212 36-37 -25.569511512910147 1212 38-39 -25.834019447575553 1212 40-41 -20.29260704858391 1212 42-43 -16.81373338479558 1212 44-45 -13.428567672651468 1212 46-47 -16.94025737108062 1212 48-49 -19.72669595971132 1212 50-51 -11.833078458803985 1212 52-53 -4.751034736285021 1212 54-55 -3.533070360616243 1212 56-57 -3.1897709762037785 1212 58-59 -2.2280847790365215 1212 60-61 -13.400081252629143 1212 62-63 -11.412613977352668 1212 64-65 -15.320600664599374 1212 66-67 -11.784590431100014 1212 68-69 -7.4277353572695795 1212 70-71 -14.436507947673267 1212 72-73 -11.29692338966521 1212 74-75 -7.954429190684689 1212 76-77 -18.13943616658949 1212 78-79 -9.51696008489861 1212 80-81 -7.435737544064885 1212 82-83 -4.909862189158204 1212 84-85 -4.230958408377678 1212 86-87 -2.9173808590047194 1212 88-89 -2.675417211569666 1212 90-91 -2.9638816262832783 1212 92-93 -2.6052953340541833 1212 94-95 -2.599529783687615 1212 96-97 -12.248960363638794 1212 98-99 -9.077575765919212 1212 100-101 -5.766741157868829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 215.0 3 434.0 4 228.0 5 1270.0 6 1643.0 7 1699.0 8 3506.0 9 6433.0 10 8364.0 11 10812.0 12 14006.0 13 17508.0 14 19867.0 15 21925.0 16 23606.0 17 25589.0 18 28897.0 19 31739.0 20 34728.0 21 38006.0 22 41640.0 23 44803.0 24 46546.0 25 47741.0 26 49636.0 27 51184.0 28 51880.0 29 51838.0 30 53550.0 31 56168.0 32 59474.0 33 66871.0 34 72913.0 35 77233.0 36 77474.0 37 67812.0 38 22577.0 39 63.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 29.705724602647006 24.04457756693056 23.35213709433734 22.89756073608509 2 28.19261168998819 30.58697311267901 19.068036914980656 22.152378282352146 3 20.71555766444692 26.896062727891067 26.817129650511397 25.571249957150616 4 24.17806411607405 26.943256665454797 25.930173974506378 22.94850524396478 5 23.919507802421723 27.021740280254274 25.281244873315423 23.77750704400858 6 23.277834997480994 27.94315131736187 24.878821277934726 23.900192407222406 7 23.769338071463288 26.89039031863353 26.216820842241372 23.12345076766181 8 23.600900754884748 26.713883559231334 25.913182368456557 23.77203331742736 9 23.038380944345825 26.03476685784536 27.027306240024245 23.899545957784575 10-11 24.035144416673397 26.101162474513046 25.740804931720586 24.12288817709297 12-13 23.820241813896477 26.526766113047053 25.689460139215765 23.96353193384071 14-15 23.91272664057884 26.552648745843843 25.5650046138056 23.969619999771723 16-17 24.082668448974477 26.473077405071223 25.50804127156523 23.936212874389074 18-19 23.924847739281248 26.661759367563427 25.60038787829898 23.813005014856344 20-21 24.056172334965666 26.499516254640877 25.507156789272805 23.937154621120655 22-23 23.996180238395397 26.372082021705705 25.58057608446756 24.051161655431333 24-25 23.797229787079814 26.569634198045023 25.797091554584554 23.83604446029061 26-27 23.902723255487555 26.44611543745189 25.63326429145596 24.017897015604596 28-29 24.029079524822627 26.383620884009947 25.694607902551986 23.892691688615443 30-31 23.900074183389826 26.447772272387425 25.738515824610996 23.91363771961175 32-33 23.952662672256324 26.535076221654126 25.59994799017685 23.9123131159127 34-35 24.005735820238897 26.329490084750688 25.686762789385 23.978011305625422 36-37 23.93156939898234 26.48084267224553 25.773802577816806 23.813785350955328 38-39 24.00507881769542 26.39332648959489 25.765768587405407 23.83582610530428 40-41 24.063555355964937 26.270210606486806 25.645242477928516 24.02099155961975 42-43 23.947397683195646 26.577554404348426 25.739045544632173 23.73600236782376 44-45 23.975550918076625 26.328867757000683 25.825877544461772 23.869703780460924 46-47 24.121310178960265 26.31865836909199 25.590414660203102 23.969616791744645 48-49 23.911447017514956 26.432529835147296 25.860312818559056 23.79571032877869 50-51 24.238341690803207 26.308916033749174 25.57740861318299 23.87533366226463 52-53 24.111067161221026 26.327225317470806 25.69130421553545 23.870403305772722 54-55 24.013822992265904 26.3714728183596 25.848324196328264 23.766379993046236 56-57 24.102056372102272 26.390536711461664 25.69620043414141 23.811206482294654 58-59 24.280467222317228 26.354202035072237 25.558108276681924 23.807222465928607 60-61 24.203683193393434 26.331759653996794 25.646394459328743 23.818162693281028 62-63 24.372515174817167 26.391502102387065 25.53410448127485 23.70187824152092 64-65 24.49751499296494 26.290991710607486 25.49822570045017 23.713267595977403 66-67 24.532243489354393 26.430992676673714 25.474135287310734 23.56262854666116 68-69 24.628850883401494 26.37012168158367 25.45903640775605 23.541991027258792 70-71 24.894406826291736 26.202464593539165 25.269844659157638 23.63328392101146 72-73 24.713167482196813 26.47171793534296 25.421429577012063 23.39368500544817 74-75 25.00607390013572 26.258660649062872 25.2456521252546 23.489613325546806 76-77 25.15439396946671 26.342988622914426 25.022972254417414 23.479645153201453 78-79 25.171521131222796 26.434773544501926 25.196636798720906 23.19706852555437 80-81 25.45382718384776 26.308706445249037 24.940100560121426 23.297365810781777 82-83 25.53932206048643 26.334333923774288 24.93659840646304 23.18974560927624 84-85 25.749636211489978 26.382467529561758 24.796239787580866 23.071656471367394 86-87 25.90083106055242 26.321610911301725 24.713206383157086 23.064351644988772 88-89 26.055896779905414 26.315761635533335 24.651723318588537 22.976618265972718 90-91 26.29039267194656 26.328030650742345 24.46708690995856 22.914489767352535 92-93 26.490032784935952 26.3468964666301 24.34099265342157 22.822078095012383 94-95 26.780094160828067 26.25979616863682 24.169783513401928 22.79032615713318 96-97 26.890098017775717 26.39446034735437 24.12019009508018 22.595251539789736 98-99 27.19831808610696 26.321643250702152 23.971829244082546 22.50820941910834 100-101 27.843263016218344 26.09237785510048 23.562094526120454 22.50226460256072 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 72.0 1 80.5 2 99.5 3 104.5 4 109.0 5 113.5 6 123.5 7 169.0 8 215.5 9 255.5 10 350.0 11 480.5 12 616.5 13 807.0 14 1033.5 15 1353.0 16 1771.5 17 2205.0 18 2734.5 19 3406.0 20 4289.5 21 5287.0 22 6425.0 23 7806.5 24 9338.0 25 11011.5 26 12811.5 27 14892.5 28 17000.5 29 19012.5 30 21019.5 31 22895.5 32 24801.0 33 26642.5 34 28533.0 35 30404.0 36 31655.5 37 32551.0 38 33350.5 39 33691.0 40 33366.5 41 32881.0 42 32185.0 43 31318.0 44 30250.0 45 28979.0 46 27934.0 47 26976.0 48 25853.0 49 24641.0 50 23757.5 51 22890.5 52 22189.0 53 21985.0 54 22096.0 55 22330.0 56 22708.5 57 23142.0 58 23652.0 59 23980.0 60 23973.5 61 23654.0 62 23138.0 63 22681.5 64 21703.0 65 20443.0 66 19138.0 67 17852.0 68 16594.5 69 15057.5 70 13545.5 71 12162.0 72 10851.5 73 9503.0 74 8283.0 75 7077.5 76 5835.5 77 4872.0 78 4137.5 79 3446.0 80 2727.0 81 2153.0 82 1759.5 83 1426.0 84 1125.5 85 906.5 86 723.0 87 591.0 88 487.0 89 373.5 90 293.0 91 227.0 92 168.5 93 125.0 94 88.5 95 63.0 96 42.0 97 28.0 98 19.0 99 6.5 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.89324794817047 2 11.252904759659089 3 9.86634446668694 4 11.88378034244047 5 9.30100383940521 6 5.424928326224228 7 9.827072278713823 8 10.201906205330936 9 5.554372059667707 10-11 6.407058261063292 12-13 5.407365608621343 14-15 7.391383535602718 16-17 2.677338727906345 18-19 3.23723166037607 20-21 3.0587180191856427 22-23 3.0618484109806015 24-25 3.534578226458234 26-27 4.412063635580115 28-29 1.7231383925885333 30-31 2.286934151192232 32-33 3.0731096905546726 34-35 2.0463818362471726 36-37 3.1478732036836177 38-39 1.3500119524050354 40-41 1.1428775862321303 42-43 0.5540386932687632 44-45 1.3282211731570122 46-47 1.4013178542912386 48-49 1.2940714444847374 50-51 1.5355588115243952 52-53 2.461992165076536 54-55 1.8975459354505082 56-57 1.8618106836613062 58-59 1.5777987735368875 60-61 1.4432732352314621 62-63 0.6064016105662513 64-65 1.21081115362662 66-67 0.575097692616666 68-69 0.8008517918037399 70-71 0.9946515020188994 72-73 0.08334159973590877 74-75 0.10248984045572 76-77 0.4532969936855526 78-79 0.9362717277648678 80-81 0.2803123561849224 82-83 0.4824868808125684 84-85 0.0649251389162177 86-87 0.09813981549389453 88-89 0.07886961145739659 90-91 0.0866752637253451 92-93 0.1648537497215171 94-95 0.4458572313676641 96-97 0.3566207379918984 98-99 0.06520971998848667 100-101 0.23103917624349735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1229878.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.91835052264445 #Duplication Level Percentage of deduplicated Percentage of total 1 99.18862016423495 98.11574697263232 2 0.6084824198400226 1.2038015458520452 3 0.13565899241656224 0.40257491290230807 4 0.05741745025449306 0.2271855788156178 5 0.007680555377977024 0.037987393454365664 6 0.0021404178759911146 0.012703596343333394 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.13088777911305E-5 0.0 0.0 2 8.13088777911305E-5 0.0 0.0 3 8.13088777911305E-5 0.0 0.0 4 8.13088777911305E-5 0.0 0.0 5 1.62617755582261E-4 0.0 0.0 6 3.25235511164522E-4 0.0 0.0 7 3.25235511164522E-4 0.0 0.0 8 3.25235511164522E-4 0.0 0.0 9 5.691621445379135E-4 0.0 0.0 10-11 6.50471022329044E-4 0.0 4.065443889556525E-5 12-13 7.317799001201745E-4 0.0 8.13088777911305E-5 14-15 9.350520945980008E-4 0.0 1.2196331668669574E-4 16-17 0.0010976698501802618 0.0 1.62617755582261E-4 18-19 0.0011789787279713922 0.0 1.62617755582261E-4 20-21 0.001300942044658088 0.0 1.62617755582261E-4 22-23 0.001300942044658088 0.0 1.62617755582261E-4 24-25 0.0017074864336137406 0.0 2.0327219447782626E-4 26-27 0.0022359941392560887 4.065443889556525E-5 2.4392663337339152E-4 28-29 0.00410609832845209 8.13088777911305E-5 2.4392663337339152E-4 30-31 0.007033217928932789 8.13088777911305E-5 2.4392663337339152E-4 32-33 0.012968766007685316 8.13088777911305E-5 2.4392663337339152E-4 34-35 0.022522559148143148 8.13088777911305E-5 2.4392663337339152E-4 36-37 0.03658899500600872 8.13088777911305E-5 2.4392663337339152E-4 38-39 0.054436293681161874 8.13088777911305E-5 2.4392663337339152E-4 40-41 0.0802518623798458 8.13088777911305E-5 2.4392663337339152E-4 42-43 0.11704412958033236 8.13088777911305E-5 2.4392663337339152E-4 44-45 0.16875657585549136 8.13088777911305E-5 2.4392663337339152E-4 46-47 0.23843828412249019 8.13088777911305E-5 2.4392663337339152E-4 48-49 0.32531682004231316 8.13088777911305E-5 2.4392663337339152E-4 50-51 0.41926922832996444 8.13088777911305E-5 2.4392663337339152E-4 52-53 0.5362727034714012 8.13088777911305E-5 2.845810722689568E-4 54-55 0.6789697839948352 8.13088777911305E-5 3.25235511164522E-4 56-57 0.8507754427674941 8.13088777911305E-5 3.25235511164522E-4 58-59 1.0553485792899784 8.13088777911305E-5 3.25235511164522E-4 60-61 1.2829321282273525 8.13088777911305E-5 3.25235511164522E-4 62-63 1.5296639178845381 8.13088777911305E-5 3.25235511164522E-4 64-65 1.8041626893073948 8.13088777911305E-5 3.25235511164522E-4 66-67 2.106306479179236 8.13088777911305E-5 3.25235511164522E-4 68-69 2.443047196551203 8.13088777911305E-5 3.6588995006008723E-4 70-71 2.804424503893882 1.62617755582261E-4 4.065443889556525E-4 72-73 3.1730383013599726 1.62617755582261E-4 4.065443889556525E-4 74-75 3.5612881928126203 1.62617755582261E-4 4.471988278512178E-4 76-77 3.977427029347626 1.62617755582261E-4 5.285077056423483E-4 78-79 4.417064131564269 2.4392663337339152E-4 5.691621445379135E-4 80-81 4.883777090085358 2.4392663337339152E-4 5.691621445379135E-4 82-83 5.37374438765471 2.4392663337339152E-4 5.691621445379135E-4 84-85 5.887657149733551 3.25235511164522E-4 5.691621445379135E-4 86-87 6.436614038140368 3.25235511164522E-4 5.691621445379135E-4 88-89 7.002198592055472 3.25235511164522E-4 5.691621445379135E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTTA 460 0.0035757644 6.9281855 6 AGGGTAG 480 0.006419449 5.6653886 92-93 GTTAGAT 545 0.0043849004 5.4028473 82-83 >>END_MODULE