FastQCFastQC Report
Thu 11 Sep 2014
NYCLib13_AGTCAA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib13_AGTCAA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229878
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTTA4600.00357576446.92818556
AGGGTAG4800.0064194495.665388692-93
GTTAGAT5450.00438490045.402847382-83
Produced by FastQC (version 0.11.2)