##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib13_AGTCAA_L007_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1229878
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247312335044615	33.0	33.0	33.0	27.0	33.0
2	31.651003595478574	33.0	33.0	33.0	27.0	33.0
3	31.793370561958177	33.0	33.0	33.0	27.0	33.0
4	35.615869216296254	37.0	37.0	37.0	33.0	37.0
5	35.12518152206967	37.0	37.0	37.0	33.0	37.0
6	34.900113669811155	37.0	33.0	37.0	33.0	37.0
7	34.70001008230085	37.0	33.0	37.0	27.0	37.0
8	34.7395717298789	37.0	33.0	37.0	27.0	37.0
9	34.65624964427366	37.0	33.0	37.0	27.0	37.0
10-11	34.49114546320854	37.0	33.0	37.0	27.0	37.0
12-13	34.48380530426596	37.0	33.0	37.0	27.0	37.0
14-15	35.37691584043296	37.0	33.0	40.0	27.0	40.0
16-17	35.28962669468028	37.0	33.0	40.0	27.0	40.0
18-19	35.49607359429147	37.0	33.0	40.0	27.0	40.0
20-21	35.149865677733885	37.0	33.0	40.0	27.0	40.0
22-23	35.161119639508954	37.0	33.0	40.0	27.0	40.0
24-25	34.97772909182862	37.0	33.0	40.0	27.0	40.0
26-27	34.564506804739985	37.0	33.0	40.0	27.0	40.0
28-29	34.164498023381185	37.0	33.0	40.0	24.5	40.0
30-31	34.02385439856636	37.0	33.0	37.0	22.0	40.0
32-33	34.018244085998774	37.0	33.0	37.0	24.5	40.0
34-35	33.83793351860916	37.0	33.0	37.0	22.0	40.0
36-37	33.50112165596913	37.0	33.0	37.0	22.0	40.0
38-39	33.371436841703	37.0	33.0	37.0	22.0	40.0
40-41	33.03800214330202	37.0	33.0	37.0	22.0	40.0
42-43	32.592442502427076	37.0	33.0	37.0	22.0	40.0
44-45	32.45019018146515	37.0	33.0	37.0	22.0	40.0
46-47	32.27420036784136	37.0	30.0	37.0	18.5	40.0
48-49	32.27124560322243	37.0	30.0	37.0	22.0	40.0
50-51	32.57857567986418	37.0	33.0	37.0	22.0	40.0
52-53	32.411037110998	37.0	33.0	37.0	22.0	40.0
54-55	32.01922995614199	37.0	27.0	37.0	18.5	40.0
56-57	32.05007732474278	33.0	27.0	37.0	22.0	40.0
58-59	31.737779682212384	33.0	27.0	37.0	15.0	40.0
60-61	31.63601877584606	33.0	27.0	37.0	18.5	40.0
62-63	31.616547332337028	33.0	27.0	37.0	22.0	40.0
64-65	31.266891106272332	33.0	27.0	37.0	15.0	40.0
66-67	30.908031934874842	33.0	27.0	37.0	15.0	40.0
68-69	30.727216032809757	33.0	27.0	37.0	15.0	37.0
70-71	30.575872159677626	33.0	27.0	37.0	15.0	37.0
72-73	30.35356149146501	33.0	27.0	37.0	15.0	37.0
74-75	30.006865721640683	33.0	27.0	37.0	15.0	37.0
76-77	28.8949290092188	33.0	27.0	33.0	15.0	37.0
78-79	29.83380749960565	33.0	27.0	37.0	15.0	37.0
80-81	29.56519264512415	33.0	27.0	37.0	15.0	37.0
82-83	29.560822292942877	33.0	27.0	37.0	15.0	37.0
84-85	29.4355932051797	33.0	27.0	37.0	15.0	37.0
86-87	29.24063646963357	33.0	27.0	37.0	15.0	37.0
88-89	29.122528006842956	33.0	27.0	33.0	15.0	37.0
90-91	29.019874328998487	33.0	27.0	33.0	15.0	37.0
92-93	28.81151789039238	33.0	27.0	33.0	15.0	37.0
94-95	28.70654406372014	33.0	27.0	33.0	15.0	37.0
96-97	28.550954240989753	33.0	27.0	33.0	15.0	37.0
98-99	28.27768973833177	33.0	27.0	33.0	15.0	37.0
100-101	27.420177041950502	33.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	1.1464952874115788
2306	2	0.5859202632156624
2306	3	0.5129836913810344
2306	4	0.2794824598589969
2306	5	0.7128130209461929
2306	6	0.8018805082450413
2306	7	0.9157598813919208
2306	8	0.8372114116560923
2306	9	1.148690648348122
2306	10-11	1.2492486623909542
2306	12-13	1.3685804708824207
2306	14-15	1.4797955254424267
2306	16-17	1.543980596954981
2306	18-19	1.3567525733723471
2306	20-21	1.5953226184927445
2306	22-23	1.2964151115271747
2306	24-25	1.2722574045371289
2306	26-27	1.3724486349367595
2306	28-29	1.494842403136957
2306	30-31	1.3871934377931652
2306	32-33	1.2562213824157311
2306	34-35	1.1579954231920695
2306	36-37	1.2205789465902797
2306	38-39	1.0089764122140465
2306	40-41	1.2987662391111314
2306	42-43	1.2478476534905596
2306	44-45	1.028602276957372
2306	46-47	0.922510578398132
2306	48-49	1.024060746939469
2306	50-51	0.8154941986768875
2306	52-53	0.9776473341623202
2306	54-55	1.4778756650664704
2306	56-57	1.00955192259433
2306	58-59	0.8781240839464459
2306	60-61	0.7148033839243304
2306	62-63	0.4647835484733598
2306	64-65	0.4659807355052372
2306	66-67	0.7013088671627834
2306	68-69	0.6661019212291919
2306	70-71	0.4199848502135133
2306	72-73	0.3439302859274882
2306	74-75	0.5168450354617562
2306	76-77	0.4477539286561445
2306	78-79	0.5276554259416066
2306	80-81	0.9676781911758532
2306	82-83	0.9534890716306208
2306	84-85	0.5947408886284116
2306	86-87	0.3467396722526459
2306	88-89	0.23052413974562214
2306	90-91	-0.23968079943853127
2306	92-93	-0.3131846853242486
2306	94-95	-0.19019931081320962
2306	96-97	-0.21744025714822612
2306	98-99	-0.0986165061862252
2306	100-101	-0.2170553991038382
2307	1	0.5182060360984444
2307	2	0.4676651193203547
2307	3	0.4175544140048295
2307	4	0.4367419721501591
2307	5	0.5392274092806844
2307	6	0.7490965297227845
2307	7	0.9005071790532639
2307	8	0.708455801257557
2307	9	0.988422828076331
2307	10-11	1.1756153121598203
2307	12-13	1.370153955337912
2307	14-15	1.3611408305087238
2307	16-17	1.3646308994217478
2307	18-19	1.2330731493715916
2307	20-21	1.3234860083460234
2307	22-23	1.102952268116148
2307	24-25	1.1920784512195937
2307	26-27	1.2580461604877797
2307	28-29	1.640956502632946
2307	30-31	1.6298740649153274
2307	32-33	1.3349988081279918
2307	34-35	1.18196790894293
2307	36-37	1.340993856993265
2307	38-39	0.8618366254203664
2307	40-41	0.9843218060375918
2307	42-43	0.9580761456596036
2307	44-45	0.7227593619183281
2307	46-47	0.666038593887798
2307	48-49	0.8454516313814437
2307	50-51	0.9178534956688509
2307	52-53	1.1164607627094938
2307	54-55	1.5114187819133846
2307	56-57	1.0881301493401203
2307	58-59	1.209123868171588
2307	60-61	0.8713004052367488
2307	62-63	0.5558439255071335
2307	64-65	0.8179054919153153
2307	66-67	0.9319101381444383
2307	68-69	0.5351329969886542
2307	70-71	0.401325874375825
2307	72-73	0.3082377805219494
2307	74-75	0.6300539016319071
2307	76-77	0.7754258505528533
2307	78-79	0.8790449972847476
2307	80-81	1.2125745104200156
2307	82-83	1.0052992526004338
2307	84-85	0.8696399306428155
2307	86-87	0.6394061954537769
2307	88-89	0.6190397427758114
2307	90-91	0.3990947207669784
2307	92-93	0.22056584636547072
2307	94-95	0.15016304104336697
2307	96-97	0.17989364774104288
2307	98-99	0.1069293688889239
2307	100-101	0.28129551596461155
1101	1	0.9434303428991022
1101	2	0.6145940042654701
1101	3	0.3387012054275367
1101	4	0.2956512490987677
1101	5	0.5152109161293197
1101	6	0.6279346449749568
1101	7	0.8493253079618484
1101	8	0.6795674376897765
1101	9	0.925444263205307
1101	10-11	1.117226450744063
1101	12-13	1.2914400196215752
1101	14-15	1.4629022157573033
1101	16-17	1.360594955870205
1101	18-19	1.0354420626840408
1101	20-21	1.400185231401906
1101	22-23	1.088733617111778
1101	24-25	1.063895780268858
1101	26-27	1.1410802056408613
1101	28-29	1.5334652305437544
1101	30-31	1.4036258454786648
1101	32-33	0.7950360431582908
1101	34-35	0.9998389852940122
1101	36-37	1.0259334416863766
1101	38-39	0.8515820725811238
1101	40-41	1.213409584007536
1101	42-43	1.10500575485257
1101	44-45	1.013299642121126
1101	46-47	0.8848847106301747
1101	48-49	0.8773002131737968
1101	50-51	0.9408327934786911
1101	52-53	1.0780326789273431
1101	54-55	1.4112311148113008
1101	56-57	1.223286457958924
1101	58-59	1.0429969834251978
1101	60-61	0.9840096657883812
1101	62-63	0.7516417960446447
1101	64-65	0.6575894649188321
1101	66-67	0.7318528935946205
1101	68-69	0.8292056512150587
1101	70-71	1.0511721744977613
1101	72-73	0.6312214672737468
1101	74-75	0.6944685051337345
1101	76-77	0.800986295264206
1101	78-79	0.7918908220103908
1101	80-81	1.470406599061004
1101	82-83	1.284125837826874
1101	84-85	1.0050503778310969
1101	86-87	1.0500576216281488
1101	88-89	0.9334093704863449
1101	90-91	0.5455755859940119
1101	92-93	0.7271456380544059
1101	94-95	0.6914616087418572
1101	96-97	0.5738300854324052
1101	98-99	0.6037820774455049
1101	100-101	0.6851233686247902
2201	1	1.4126469254941263
2201	2	0.7545608555298031
2201	3	0.35548125311098033
2201	4	0.2193020321390975
2201	5	0.3169492536533767
2201	6	0.38811965399915493
2201	7	0.6328078448856616
2201	8	0.5325166296250643
2201	9	0.4572320838730448
2201	10-11	0.8520069989151438
2201	12-13	1.0906840604764483
2201	14-15	1.0431302118699506
2201	16-17	0.9247010798163089
2201	18-19	0.9079376962722492
2201	20-21	1.1494816349183452
2201	22-23	0.7865714933987746
2201	24-25	0.7892039143926937
2201	26-27	1.1194726805007917
2201	28-29	1.4206669733376742
2201	30-31	1.3486707608156934
2201	32-33	0.9264877486727414
2201	34-35	0.8409243064373797
2201	36-37	0.965417716604918
2201	38-39	0.6642505800824381
2201	40-41	1.0965645757717652
2201	42-43	1.1401380155654905
2201	44-45	0.984450472296416
2201	46-47	0.8109655153868687
2201	48-49	0.9213444103453554
2201	50-51	0.9812330293249261
2201	52-53	1.2365445680796157
2201	54-55	1.3546760123227415
2201	56-57	1.154144605709046
2201	58-59	1.2117894370315412
2201	60-61	0.9736451153959642
2201	62-63	0.8071916382710569
2201	64-65	0.854396799462247
2201	66-67	0.8569932272416168
2201	68-69	0.7647802950032698
2201	70-71	0.907728048285751
2201	72-73	0.7541006080804564
2201	74-75	0.9155409198665794
2201	76-77	0.9231387006043406
2201	78-79	1.0457443196819263
2201	80-81	1.3342478245015421
2201	82-83	1.2464979949457131
2201	84-85	1.1721181202033755
2201	86-87	1.030179092718992
2201	88-89	1.119845298642371
2201	90-91	0.9827965255738427
2201	92-93	1.024040215133784
2201	94-95	0.9226170495737627
2201	96-97	0.8691700656528596
2201	98-99	0.8392692215979416
2201	100-101	0.9502558350175612
2304	1	-1.6983335351500095
2304	2	-0.16955695551563466
2304	3	-0.22778437459520973
2304	4	-0.10446229948794894
2304	5	0.32394996126306097
2304	6	0.5405732010689377
2304	7	0.599433219255296
2304	8	0.7483276381803847
2304	9	0.7284217756958284
2304	10-11	0.7576572640066317
2304	12-13	1.12554350937976
2304	14-15	1.118475546726252
2304	16-17	1.0500286249964788
2304	18-19	0.7910353887126149
2304	20-21	1.2337027345589675
2304	22-23	0.9524159299125969
2304	24-25	1.0459543190897378
2304	26-27	0.9497326845721688
2304	28-29	1.2963842100513716
2304	30-31	1.5408605506263342
2304	32-33	0.9124186278262414
2304	34-35	0.982060166410939
2304	36-37	1.184110417667128
2304	38-39	0.8735604925116718
2304	40-41	0.926757326189481
2304	42-43	0.8039786728578662
2304	44-45	1.1261389150453027
2304	46-47	0.9602772140152922
2304	48-49	0.9742303694818872
2304	50-51	0.998201921617472
2304	52-53	1.085080481349877
2304	54-55	1.4868533059706337
2304	56-57	1.0252008207240415
2304	58-59	0.8875601150893857
2304	60-61	0.4613960013543803
2304	62-63	0.3384536494662598
2304	64-65	0.4976306299302493
2304	66-67	0.3431370762983832
2304	68-69	0.35729022666849275
2304	70-71	0.3602441665639553
2304	72-73	0.3703722318476075
2304	74-75	0.5834322538717274
2304	76-77	0.6187092520372985
2304	78-79	0.5615797528840432
2304	80-81	1.1971954545153274
2304	82-83	0.8616263969952875
2304	84-85	0.5512314856376719
2304	86-87	0.5727717174519213
2304	88-89	0.3008944845658199
2304	90-91	0.21392030520025074
2304	92-93	0.24275072395106534
2304	94-95	0.09475584954440208
2304	96-97	0.23297716843416083
2304	98-99	0.6095198933209254
2304	100-101	0.6076702429919614
1102	1	1.4549113236841045
1102	2	-0.03432815923870791
1102	3	0.18349884730591626
1102	4	0.15297212493440782
1102	5	0.2208434656768361
1102	6	0.14208604225157018
1102	7	-5.306583476784294
1102	8	-1.3746073388307565
1102	9	0.07392532687516251
1102	10-11	0.5017788106281813
1102	12-13	0.9452907354322946
1102	14-15	0.860333881009204
1102	16-17	0.6802875388193641
1102	18-19	0.7425434365718857
1102	20-21	1.0724187589906506
1102	22-23	0.7868934305351232
1102	24-25	1.1652189368669212
1102	26-27	1.0933521079625024
1102	28-29	0.8582895289520707
1102	30-31	1.1211457021164506
1102	32-33	0.978992060836589
1102	34-35	1.1707670764940232
1102	36-37	1.1477660733565784
1102	38-39	0.9962964008946855
1102	40-41	1.1611561640547734
1102	42-43	1.1375023322798796
1102	44-45	1.3454916415418907
1102	46-47	0.9930065685083846
1102	48-49	0.9116933769837914
1102	50-51	0.6352289678188754
1102	52-53	0.8470209311907126
1102	54-55	1.229309348582781
1102	56-57	1.131227826930843
1102	58-59	0.9039245411135397
1102	60-61	0.7892933844409988
1102	62-63	0.7463736063926731
1102	64-65	0.4911724055018283
1102	66-67	0.5553596086965271
1102	68-69	0.7999370576185321
1102	70-71	0.5429503784497491
1102	72-73	0.5880055922526637
1102	74-75	0.6429498546134127
1102	76-77	0.5166822088505576
1102	78-79	0.6952706661297121
1102	80-81	1.3171868084452818
1102	82-83	1.0887943508250295
1102	84-85	0.8171399430095541
1102	86-87	0.5667508209738372
1102	88-89	0.35183876926631896
1102	90-91	0.2299496005455417
1102	92-93	0.36496408974635486
1102	94-95	0.2535797549030967
1102	96-97	0.053696926633609365
1102	98-99	0.24279759596646144
1102	100-101	0.46055782338061846
2202	1	1.4367710319651614
2202	2	0.8518602817901524
2202	3	0.402299819702403
2202	4	-0.18814952918521044
2202	5	0.11866929691899486
2202	6	0.4960285610721442
2202	7	0.6877246056080111
2202	8	-4.275580388809846
2202	9	-0.5113462645092
2202	10-11	0.5702138273575841
2202	12-13	0.07464462763220325
2202	14-15	0.060913201900049785
2202	16-17	0.3876919329013333
2202	18-19	0.8307397325777401
2202	20-21	1.0397098375142804
2202	22-23	-3.016607000769767
2202	24-25	-3.2376554488503544
2202	26-27	-1.5287761202892796
2202	28-29	0.5642353283038091
2202	30-31	1.2775064690549698
2202	32-33	1.2066978455500958
2202	34-35	-2.1606387814106185
2202	36-37	-0.5420685277517379
2202	38-39	0.10331719501847658
2202	40-41	1.1769915885633395
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2202	60-61	1.192125293161201
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2202	68-69	1.3055557840928138
2202	70-71	1.2985893631841705
2202	72-73	1.1351383949502818
2202	74-75	0.7014155201299346
2202	76-77	0.9910839366840989
2202	78-79	1.0975719391326386
2202	80-81	1.8158223166008653
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2202	84-85	1.4979070122274543
2202	86-87	1.4235105449387042
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2202	90-91	1.0641488111268878
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2202	94-95	1.010155847542162
2202	96-97	1.1335141448477444
2202	98-99	1.1675770423278173
2202	100-101	1.081616938290697
2305	1	1.3918429444935576
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1103	2	0.5886003774185653
1103	3	0.2464425792652456
1103	4	-0.294245840660615
1103	5	0.18939163298456663
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1103	9	0.12541944587331244
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1103	70-71	0.5882324642770662
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1209	94-95	-1.3452001847961483
1209	96-97	0.1322353824099345
1209	98-99	1.0042569241686117
1209	100-101	0.3221758624200497
1212	1	-28.632499801368173
1212	2	-16.300743884876518
1212	3	-5.900664923887341
1212	4	-1.5254091445698208
1212	5	-0.6562105107733132
1212	6	-2.728049964568882
1212	7	-0.4553042405458356
1212	8	-1.216886478553434
1212	9	-0.14219562348355907
1212	10-11	0.7477779299216891
1212	12-13	-0.048452327496001146
1212	14-15	-0.2908696506640496
1212	16-17	-0.31248835556019827
1212	18-19	-1.179015876396619
1212	20-21	-1.1084271817164861
1212	22-23	-0.8248401738466882
1212	24-25	0.18183573704708778
1212	26-27	-0.7679788446482547
1212	28-29	0.12599414240637685
1212	30-31	-0.6345728578681076
1212	32-33	0.2992258904218872
1212	34-35	-1.474961698077287
1212	36-37	-1.9758625982645626
1212	38-39	-1.268778157545622
1212	40-41	-1.327335334513581
1212	42-43	-0.22484957631967717
1212	44-45	1.5161707507012352
1212	46-47	-2.455111675386995
1212	48-49	-1.497208796229863
1212	50-51	-0.4754660865516769
1212	52-53	-1.0374486743814657
1212	54-55	-0.5213169986166548
1212	56-57	-1.3083723966640513
1212	58-59	-0.5709310873650537
1212	60-61	-0.2572136491464896
1212	62-63	-4.798937536354167
1212	64-65	-3.634539901064464
1212	66-67	-1.647236565257085
1212	68-69	0.452911553323581
1212	70-71	-0.6089026239953164
1212	72-73	-1.1986156114599744
1212	74-75	-0.34499874269057784
1212	76-77	0.765663263607177
1212	78-79	-1.6691548236878724
1212	80-81	0.345149239677788
1212	82-83	0.1969704438662454
1212	84-85	-3.237389462131521
1212	86-87	-1.9495663781382184
1212	88-89	-1.041808381105266
1212	90-91	-0.24254189400131665
1212	92-93	-2.6734305914298524
1212	94-95	-2.0169675498937387
1212	96-97	-0.13473505387020168
1212	98-99	-0.804798758954238
1212	100-101	-0.05201687581186931
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	9.0
12	32.0
13	145.0
14	484.0
15	1195.0
16	2086.0
17	3549.0
18	5318.0
19	7612.0
20	10567.0
21	13789.0
22	17812.0
23	22375.0
24	27966.0
25	33491.0
26	40308.0
27	47687.0
28	55938.0
29	64744.0
30	74865.0
31	85427.0
32	97189.0
33	107610.0
34	118137.0
35	125676.0
36	125036.0
37	105799.0
38	34962.0
39	65.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	28.006429262149645	23.516180828320195	26.093371686191087	22.384018223339073
2	28.056800515185614	29.752863768530744	20.21851480833404	21.9718209079496
3	20.718219915210824	25.944690269084475	28.487552383414886	24.84953743228981
4	24.213842870210385	26.459843480028454	27.10706372598841	22.21924992377274
5	23.577867072994234	27.066749710133852	25.96501441606403	23.390368800807884
6	22.71225276002986	28.16490741358086	25.64311256888895	23.479727257500336
7	23.072451088644566	27.191396219787656	26.868274739445702	22.867877952122083
8	23.126440183497877	26.679475525214695	26.51311756125404	23.680966730033383
9	22.689730200881712	25.84784832316701	27.56509182211569	23.897329653835584
10-11	23.50883583574956	26.03087460707485	26.32244824283384	24.13784131434175
12-13	23.584854167718934	26.318364227954895	26.130544372084536	23.966237232241635
14-15	23.57404555573805	26.434817111941182	25.957777925940622	24.033359406380146
16-17	23.546477118794282	26.335380697534873	26.02421061019259	24.093931573478258
18-19	23.641460618322075	26.509674738479056	25.99825238933054	23.850612253868327
20-21	23.608927064310446	26.292770502440078	26.00684783368757	24.091454599561906
22-23	23.601853191942617	26.234268764869363	26.02453251460714	24.139345528580883
24-25	23.531033159386542	26.217925680433346	26.243822558009818	24.007218602170298
26-27	23.798945911708316	26.266141844963485	25.973429884915415	23.961482358412788
28-29	23.829111505368825	26.230894446441027	26.05477941714544	23.885214631044704
30-31	23.705196775615143	26.226422458162517	26.180117052260464	23.888263713961873
32-33	23.829233468685512	26.19524050352962	25.911431865599678	24.064094162185192
34-35	23.832851713747218	26.06811407310319	26.038883531537277	24.06015068161232
36-37	23.759836341490782	26.239797768559157	26.09677545252456	23.9035904374255
38-39	23.84602375194938	26.15918001622925	26.088766528062134	23.906029703759234
40-41	23.87182097445396	25.980153806374396	26.058170943312152	24.08985427585949
42-43	23.657915663179597	26.302974766602865	26.232032770730108	23.80707679948743
44-45	23.798051514052613	25.94737038958336	26.248091274093856	24.006486822270176
46-47	23.824569450679576	26.053194880903586	26.135539268310136	23.986696400106702
48-49	23.85035251492629	26.133629623124914	26.194001513972538	23.82201634797626
50-51	23.9181544503416	26.07918268282817	26.013647700685638	23.989015166144593
52-53	23.85458058000922	26.087527451932186	26.18570800169448	23.872183966364116
54-55	23.62958605333224	26.06861044357614	26.467112220002903	23.83469128308872
56-57	23.93091577015614	26.16954273694344	26.013386878679785	23.88615461422063
58-59	23.855051527513556	26.144683967974608	26.142323773868988	23.85794073064285
60-61	23.906395469384602	26.16759699314147	26.090718011472898	23.835289526001034
62-63	24.058092861902406	26.162034087661308	25.89293809003231	23.886934960403984
64-65	24.00799773305411	26.069023948884762	26.016131255191187	23.906847062869936
66-67	23.974103674744494	26.29337297034461	25.968408564781875	23.764114790129018
68-69	24.119445024926392	26.25060882843926	25.745535325872627	23.88441082076172
70-71	24.141419028332674	26.192345392815046	25.68601710230116	23.980218476551112
72-73	23.92775991224991	26.422878468001276	25.871146139927546	23.778215479821263
74-75	24.008023824185194	26.25036706718	25.775884438710563	23.965724669924242
76-77	23.956606287234976	26.35764145627241	25.71338879424631	23.972363462246296
78-79	23.989174496401457	26.534354956017836	25.58612735374817	23.890343193832535
80-81	24.016396978801303	26.35946208402149	25.575021631737076	24.04911930544013
82-83	23.991977697576434	26.461645043609987	25.4412937162019	24.105083542611673
84-85	23.84570224675704	26.48601377278083	25.656459456976123	24.011824523486013
86-87	23.922670352902912	26.527931370954626	25.36245731013173	24.186940966010734
88-89	23.93311121094647	26.564346714208178	25.287027795113442	24.21551427973191
90-91	23.88921826242043	26.638186022037935	25.268236111212772	24.20435960432886
92-93	23.917052789763087	26.534300579143988	25.226591507514563	24.322055123578366
94-95	23.94738094008611	26.549180637818864	25.127133994981733	24.3763044271133
96-97	23.855986592511304	26.67795883832665	25.139691834304323	24.326362734857724
98-99	23.845127671763564	26.63902115536227	25.050752783830344	24.465098389043824
100-101	23.833735522707265	26.444029266646474	24.97606176978233	24.74617344086393
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	5.5
5	10.5
6	15.0
7	27.5
8	42.5
9	52.5
10	79.0
11	116.0
12	176.0
13	249.0
14	338.0
15	474.5
16	669.0
17	963.5
18	1339.5
19	1889.0
20	2636.5
21	3522.5
22	4639.0
23	5933.0
24	7432.5
25	9105.5
26	11011.0
27	13045.5
28	15279.0
29	17566.0
30	19662.0
31	21759.0
32	23966.0
33	26172.0
34	28152.5
35	29939.0
36	31519.5
37	32685.0
38	33773.0
39	34462.5
40	34401.0
41	34155.0
42	33299.0
43	32278.5
44	31354.0
45	30244.5
46	29171.5
47	28064.0
48	26851.0
49	25544.0
50	24167.0
51	23273.5
52	22828.0
53	22492.0
54	22541.0
55	22864.5
56	23461.0
57	24172.0
58	24991.5
59	25652.0
60	25846.0
61	25682.0
62	25228.0
63	24572.0
64	23617.0
65	22528.0
66	21226.0
67	19761.0
68	18280.5
69	16706.0
70	15132.0
71	13524.5
72	11888.5
73	10197.0
74	8528.5
75	6974.5
76	5575.5
77	4189.0
78	3016.0
79	2212.0
80	1543.5
81	1035.0
82	704.0
83	465.0
84	302.5
85	210.5
86	132.5
87	88.5
88	62.0
89	44.0
90	26.0
91	19.0
92	19.5
93	12.5
94	8.5
95	3.0
96	1.5
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6981993335924377
2	0.0024392663337339153
3	3.25235511164522E-4
4	2.439266333733915E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.19286465812056156
14-15	0.0
16-17	3.25235511164522E-4
18-19	0.10923847731238383
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0016261775558226099
42-43	0.0
44-45	0.0
46-47	0.023254339048263326
48-49	8.13088777911305E-5
50-51	4.065443889556525E-5
52-53	8.13088777911305E-5
54-55	0.0017481408725093057
56-57	0.0013822509224492186
58-59	0.0947654970655626
60-61	0.0018701041891960018
62-63	0.001585523116927045
64-65	0.00239861189483835
66-67	0.004146752767347656
68-69	0.0046346060340944385
70-71	0.0035369361839141765
72-73	0.03882498914526482
74-75	0.04382548512941934
76-77	0.15172236595824953
78-79	3.6588995006008723E-4
80-81	0.11049876491814635
82-83	0.0045939515951988735
84-85	0.004959841545258961
86-87	0.00634209246770818
88-89	0.006138820273230354
90-91	0.022034705881396364
92-93	0.010732771868429226
94-95	0.01394447254117888
96-97	0.009553793140457834
98-99	0.011586515085236096
100-101	0.012236986107565139
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1229878.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.96401280032188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.76394570940533	96.7531244168648
2	0.7871098385989101	1.5421687660752585
3	0.21828433284228088	0.6415202753001278
4	0.12237962777058925	0.4795519768566652
5	0.07237421846203455	0.3545034431914022
6	0.025931918747770805	0.15242368920861152
7	0.005574874907631407	0.03822959817679775
8	0.0016312767261720267	0.012784513126686686
9	0.0014547196047705897	0.012825915298436267
>10	0.0013134829345388306	0.012867405901216634
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	8.13088777911305E-5	0.0	0.0
2	8.13088777911305E-5	0.0	0.0
3	1.62617755582261E-4	0.0	0.0
4	1.62617755582261E-4	0.0	0.0
5	4.065443889556525E-4	0.0	0.0
6	5.691621445379135E-4	0.0	0.0
7	5.691621445379135E-4	0.0	0.0
8	5.691621445379135E-4	0.0	0.0
9	8.13088777911305E-4	0.0	0.0
10-11	9.350520945980008E-4	0.0	0.0
12-13	0.0010976698501802618	0.0	0.0
14-15	0.0013415964835536533	0.0	0.0
16-17	0.0015042142391359144	0.0	4.065443889556525E-5
18-19	0.0017887953114048712	0.0	8.13088777911305E-5
20-21	0.0019514130669871321	0.0	8.13088777911305E-5
22-23	0.0019920675058826974	4.065443889556525E-5	8.13088777911305E-5
24-25	0.002601884089316176	8.13088777911305E-5	8.13088777911305E-5
26-27	0.00325235511164522	8.13088777911305E-5	8.13088777911305E-5
28-29	0.005813584762065831	8.13088777911305E-5	1.2196331668669574E-4
30-31	0.009391175384875574	8.13088777911305E-5	1.62617755582261E-4
32-33	0.01740009984730193	8.13088777911305E-5	1.62617755582261E-4
34-35	0.02825483503241785	8.13088777911305E-5	1.62617755582261E-4
36-37	0.042890433034821346	8.13088777911305E-5	1.62617755582261E-4
38-39	0.06309568916591728	8.13088777911305E-5	1.62617755582261E-4
40-41	0.09374913609317348	8.13088777911305E-5	1.62617755582261E-4
42-43	0.13550124483891898	8.13088777911305E-5	1.62617755582261E-4
44-45	0.19262073148718817	8.13088777911305E-5	1.62617755582261E-4
46-47	0.26998612870544886	8.13088777911305E-5	1.62617755582261E-4
48-49	0.3657679867434006	8.13088777911305E-5	1.62617755582261E-4
50-51	0.4745186107890376	8.13088777911305E-5	1.62617755582261E-4
52-53	0.6037184175991439	8.13088777911305E-5	1.62617755582261E-4
54-55	0.7547090036572733	1.2196331668669574E-4	1.62617755582261E-4
56-57	0.9419633492102468	1.62617755582261E-4	1.62617755582261E-4
58-59	1.1547080279507398	1.62617755582261E-4	1.62617755582261E-4
60-61	1.3973337192794733	1.62617755582261E-4	1.62617755582261E-4
62-63	1.6598800856670337	1.62617755582261E-4	1.62617755582261E-4
64-65	1.9510878314759674	1.62617755582261E-4	1.62617755582261E-4
66-67	2.270184522367259	1.62617755582261E-4	1.62617755582261E-4
68-69	2.627049186992531	1.62617755582261E-4	1.62617755582261E-4
70-71	3.009770074755382	1.62617755582261E-4	1.62617755582261E-4
72-73	3.420786451989547	1.62617755582261E-4	1.62617755582261E-4
74-75	3.8412753134863786	1.62617755582261E-4	1.62617755582261E-4
76-77	4.302621886073252	1.62617755582261E-4	1.62617755582261E-4
78-79	4.795556957681981	1.62617755582261E-4	1.62617755582261E-4
80-81	5.314510870183872	1.62617755582261E-4	2.0327219447782626E-4
82-83	5.86208550766824	1.62617755582261E-4	2.4392663337339152E-4
84-85	6.433199065273142	1.62617755582261E-4	2.4392663337339152E-4
86-87	7.042974994267723	1.62617755582261E-4	2.4392663337339152E-4
88-89	7.678688455277678	1.62617755582261E-4	2.4392663337339152E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGTA	1020	0.0	9.30675	94-95
CTCGTAT	845	0.0	9.268201	94-95
AATCTCG	1520	0.0	7.338548	92-93
TCGTATG	870	7.4578566E-11	7.092386	94-95
CGTATGC	775	2.4956535E-9	7.0431085	94-95
GTCAAAT	2340	0.0	6.9979606	94-95
TAATTCC	705	0.007689827	6.778163	1
ATCTCGT	1385	0.0	6.682717	94-95
GTATGCC	930	3.5470293E-10	6.636566	90-91
AGTCAAA	2700	0.0	6.5922813	94-95
TATGCCG	735	3.4278855E-7	6.457745	94-95
GCCGTCT	755	5.5615965E-7	6.2866793	94-95
CAGTCAA	2790	0.0	6.039626	92-93
TCAAATC	2455	0.0	5.9934716	94-95
CACAGTC	3140	0.0	5.9708433	94-95
TCCAGTC	4620	0.0	5.804632	94-95
ACAGTCA	3070	0.0	5.798008	92-93
CTCCAGT	4835	0.0	5.644684	94-95
AAATCTC	2200	0.0	5.609432	94-95
CAAATCT	2245	0.0	5.6027045	94-95
>>END_MODULE