FastQCFastQC Report
Thu 11 Sep 2014
NYCLib13_AGTCAA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib13_AGTCAA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229878
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA10200.09.3067594-95
CTCGTAT8450.09.26820194-95
AATCTCG15200.07.33854892-93
TCGTATG8707.4578566E-117.09238694-95
CGTATGC7752.4956535E-97.043108594-95
GTCAAAT23400.06.997960694-95
TAATTCC7050.0076898276.7781631
ATCTCGT13850.06.68271794-95
GTATGCC9303.5470293E-106.63656690-91
AGTCAAA27000.06.592281394-95
TATGCCG7353.4278855E-76.45774594-95
GCCGTCT7555.5615965E-76.286679394-95
CAGTCAA27900.06.03962692-93
TCAAATC24550.05.993471694-95
CACAGTC31400.05.970843394-95
TCCAGTC46200.05.80463294-95
ACAGTCA30700.05.79800892-93
CTCCAGT48350.05.64468494-95
AAATCTC22000.05.60943294-95
CAAATCT22450.05.602704594-95
Produced by FastQC (version 0.11.2)