##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib12_GGCTAC_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1926793 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.163934579376196 33.0 22.0 33.0 2.0 33.0 2 25.230466894990794 33.0 15.0 33.0 2.0 33.0 3 26.105116636815684 33.0 22.0 33.0 6.0 33.0 4 28.25716566335875 33.0 22.0 37.0 2.0 37.0 5 28.27547069145466 33.0 22.0 37.0 6.0 37.0 6 29.360932388689392 33.0 27.0 37.0 15.0 37.0 7 29.044087766563404 33.0 27.0 37.0 6.0 37.0 8 28.822447974432126 33.0 27.0 37.0 6.0 37.0 9 29.552006364980567 33.0 27.0 37.0 15.0 37.0 10-11 29.151931473697488 33.0 27.0 37.0 10.5 37.0 12-13 29.44330786960509 33.0 27.0 37.0 15.0 37.0 14-15 29.781269186674436 33.0 27.0 37.0 6.0 40.0 16-17 30.621418336064124 33.0 27.0 37.0 15.0 40.0 18-19 30.909338470712733 33.0 27.0 37.0 15.0 40.0 20-21 30.922515547855944 33.0 27.0 37.0 15.0 40.0 22-23 30.920716444371553 33.0 27.0 37.0 15.0 40.0 24-25 30.494615664474594 33.0 27.0 37.0 10.5 40.0 26-27 29.98924663936396 33.0 27.0 37.0 15.0 40.0 28-29 30.138282887679164 33.0 27.0 37.0 15.0 40.0 30-31 30.024499777609737 33.0 27.0 37.0 15.0 40.0 32-33 29.867555829816695 33.0 27.0 37.0 15.0 40.0 34-35 29.884178009781017 33.0 27.0 37.0 15.0 40.0 36-37 30.134691687171376 33.0 27.0 37.0 15.0 40.0 38-39 30.152216143612726 33.0 27.0 37.0 15.0 40.0 40-41 30.208043365322585 33.0 27.0 37.0 15.0 40.0 42-43 30.094747853038704 33.0 27.0 37.0 15.0 40.0 44-45 29.8356595648832 33.0 27.0 37.0 15.0 40.0 46-47 29.452645406123025 33.0 27.0 37.0 15.0 40.0 48-49 29.281128538457427 33.0 24.5 37.0 10.5 40.0 50-51 28.480808784337498 33.0 22.0 37.0 10.5 38.5 52-53 28.180103934361398 33.0 22.0 37.0 10.5 37.0 54-55 29.117552845583308 33.0 22.0 37.0 15.0 40.0 56-57 29.016608686039444 33.0 22.0 37.0 10.5 40.0 58-59 29.191031678026647 33.0 24.5 37.0 15.0 40.0 60-61 28.933143570689744 33.0 24.5 37.0 10.5 40.0 62-63 28.78548422170934 33.0 22.0 37.0 15.0 40.0 64-65 28.73465598017016 33.0 22.0 37.0 10.5 40.0 66-67 28.571481991059756 33.0 22.0 37.0 15.0 40.0 68-69 28.260378774471363 33.0 22.0 37.0 6.0 38.5 70-71 28.128657567263325 33.0 22.0 37.0 6.0 37.0 72-73 27.839015659699825 33.0 22.0 37.0 6.0 37.0 74-75 27.743333352363226 33.0 22.0 37.0 10.5 37.0 76-77 27.310436045802533 30.0 22.0 35.0 6.0 37.0 78-79 26.68703176729415 27.0 22.0 33.0 6.0 37.0 80-81 26.510977307889327 27.0 22.0 33.0 6.0 37.0 82-83 26.017714928380993 27.0 22.0 33.0 6.0 37.0 84-85 26.033143155492056 27.0 22.0 33.0 6.0 37.0 86-87 25.651821186811453 27.0 22.0 33.0 6.0 37.0 88-89 25.201787114651133 27.0 15.0 33.0 6.0 37.0 90-91 24.970892047043975 27.0 15.0 33.0 6.0 37.0 92-93 24.759962019791438 27.0 15.0 33.0 6.0 37.0 94-95 24.429752962565257 27.0 15.0 33.0 6.0 37.0 96-97 24.180463339860587 27.0 15.0 33.0 6.0 37.0 98-99 23.82051003922061 27.0 15.0 33.0 6.0 37.0 100-101 23.20677078440704 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.425965162244065 2306 2 8.634045161295536 2306 3 7.601283922709744 2306 4 9.27210912066236 2306 5 9.001340867236593 2306 6 8.012919200675363 2306 7 8.4079527821944 2306 8 8.71819871457961 2306 9 7.547689522730355 2306 10-11 7.877126579897233 2306 12-13 7.507283336945498 2306 14-15 8.127485807771613 2306 16-17 6.8983366785640925 2306 18-19 6.213587784492116 2306 20-21 6.113694741102474 2306 22-23 5.765200986669573 2306 24-25 5.745963204512911 2306 26-27 6.461710347361038 2306 28-29 5.624009256898201 2306 30-31 5.5693689397541775 2306 32-33 5.38261403428951 2306 34-35 4.79723108799632 2306 36-37 4.747314541563178 2306 38-39 4.352370603931934 2306 40-41 3.7747563175348553 2306 42-43 3.388180569390581 2306 44-45 3.009390148380472 2306 46-47 3.460650724050872 2306 48-49 3.138568809934956 2306 50-51 3.0043970031705847 2306 52-53 3.6495451484142833 2306 54-55 3.068686486960747 2306 56-57 3.361684727411067 2306 58-59 2.968066611515919 2306 60-61 2.913467668225355 2306 62-63 2.4295186638130772 2306 64-65 2.1575203061343267 2306 66-67 2.036408361537095 2306 68-69 2.1410473905935916 2306 70-71 2.2402270493960366 2306 72-73 1.3390735286635156 2306 74-75 1.1487021845337289 2306 76-77 1.4550188983340746 2306 78-79 1.5918192826178128 2306 80-81 1.3486760359112715 2306 82-83 1.273643970145823 2306 84-85 0.8593727123754711 2306 86-87 0.7186887875704997 2306 88-89 0.7521310284687388 2306 90-91 0.661225598214628 2306 92-93 0.5979139577313539 2306 94-95 0.8233718674692874 2306 96-97 0.27773602581346424 2306 98-99 0.26445930230805814 2306 100-101 0.14010246743984212 2307 1 8.432032880220238 2307 2 8.578212177295406 2307 3 7.700490964875769 2307 4 9.254735398704195 2307 5 9.208577714168975 2307 6 7.762225466514586 2307 7 8.322074697117543 2307 8 8.703763467748193 2307 9 7.634663625441323 2307 10-11 7.92160855829874 2307 12-13 7.544355456764585 2307 14-15 8.267840550950162 2307 16-17 7.048216763930199 2307 18-19 6.581971099784038 2307 20-21 6.513534512520348 2307 22-23 6.218913236875952 2307 24-25 6.328851861545651 2307 26-27 7.0100735521479045 2307 28-29 6.176634978941802 2307 30-31 6.074400234628406 2307 32-33 6.326128200749515 2307 34-35 5.902839936227235 2307 36-37 5.697337719026237 2307 38-39 5.302882684827882 2307 40-41 4.472077074546835 2307 42-43 4.498031553711268 2307 44-45 4.489759415993838 2307 46-47 4.790275663294608 2307 48-49 4.570039337218571 2307 50-51 4.342256423011701 2307 52-53 4.919290708295534 2307 54-55 4.62741938491974 2307 56-57 4.932723610927486 2307 58-59 4.444011943030272 2307 60-61 4.370725100136358 2307 62-63 4.100341784143648 2307 64-65 3.9065333277434178 2307 66-67 3.6424368172332464 2307 68-69 3.6700618568197925 2307 70-71 3.2353951638592804 2307 72-73 2.7478995196406935 2307 74-75 2.48215731572078 2307 76-77 2.757731240337229 2307 78-79 3.0105797880903182 2307 80-81 2.693804358184149 2307 82-83 2.8461044808196014 2307 84-85 2.034894425410922 2307 86-87 1.8736694637784872 2307 88-89 2.121281490884808 2307 90-91 1.9816126512495202 2307 92-93 1.762684768247972 2307 94-95 1.9136391591366682 2307 96-97 1.7633233613150274 2307 98-99 1.4521318594580706 2307 100-101 1.1307599850821823 1101 1 8.121391966349016 1101 2 7.9610121931341915 1101 3 7.297258744754199 1101 4 8.850660904052138 1101 5 8.681882477281782 1101 6 7.404191174196335 1101 7 7.1454196947786315 1101 8 8.206933070966418 1101 9 7.542525838724028 1101 10-11 7.613404116458295 1101 12-13 7.2844020201167154 1101 14-15 7.764666249786352 1101 16-17 6.898202720428564 1101 18-19 5.90055808526672 1101 20-21 5.833514354978096 1101 22-23 5.335080260250923 1101 24-25 5.9726654557894605 1101 26-27 6.5581035022221705 1101 28-29 5.982971567951434 1101 30-31 5.749169515164887 1101 32-33 5.67458094914835 1101 34-35 5.360035276133662 1101 36-37 5.217479489601395 1101 38-39 4.81501261255335 1101 40-41 4.4468014561461935 1101 42-43 4.195471190143966 1101 44-45 4.080751491230764 1101 46-47 4.528994637558597 1101 48-49 4.183926514712997 1101 50-51 3.585776529883251 1101 52-53 4.550823201636199 1101 54-55 4.446842017473955 1101 56-57 4.466816518170571 1101 58-59 4.007557697329062 1101 60-61 4.131967116145617 1101 62-63 3.7423975078185414 1101 64-65 3.466925418282223 1101 66-67 3.296497301940427 1101 68-69 3.4261875584076336 1101 70-71 3.4057661606223952 1101 72-73 2.8505940095545377 1101 74-75 2.356594568952797 1101 76-77 2.534511963055756 1101 78-79 2.986780750766485 1101 80-81 2.6042558106211438 1101 82-83 2.6629241998421875 1101 84-85 1.910063803127759 1101 86-87 2.206649696165723 1101 88-89 2.1325301343530185 1101 90-91 2.0898216064734 1101 92-93 1.735417556485391 1101 94-95 1.9793088043008389 1101 96-97 1.911928608383338 1101 98-99 1.6773067404825994 1101 100-101 1.2724241853274165 2201 1 7.225389972230317 2201 2 7.968847866974521 2201 3 6.419705660887807 2201 4 8.493364854899806 2201 5 3.7854987823460107 2201 6 5.894958438312642 2201 7 6.746553007676745 2201 8 7.833659258034064 2201 9 6.732114765077501 2201 10-11 4.112934190485461 2201 12-13 6.528550528064564 2201 14-15 7.248548302605791 2201 16-17 6.14517041929868 2201 18-19 1.9125687767071646 2201 20-21 5.008105201344502 2201 22-23 5.132566250331973 2201 24-25 5.609111708339647 2201 26-27 6.328507513364986 2201 28-29 5.252040945541619 2201 30-31 5.357187983219127 2201 32-33 5.334772933599616 2201 34-35 4.993916093857063 2201 36-37 5.063653555054749 2201 38-39 4.5034040010146335 2201 40-41 4.221559124689083 2201 42-43 4.077854481989302 2201 44-45 3.7821164688474553 2201 46-47 4.33947237832092 2201 48-49 3.8927374372286856 2201 50-51 3.7299327010406174 2201 52-53 4.50088807224866 2201 54-55 4.078546006497323 2201 56-57 4.164999776106907 2201 58-59 3.8925830560963917 2201 60-61 3.7859381183362295 2201 62-63 3.6503303893312875 2201 64-65 3.5145692480231148 2201 66-67 3.0523677976293833 2201 68-69 3.2448668421417537 2201 70-71 3.1613632228903263 2201 72-73 2.4481496702803973 2201 74-75 2.1282316831396706 2201 76-77 2.41125339233135 2201 78-79 2.661075286364756 2201 80-81 2.1731106183447615 2201 82-83 2.452140576317422 2201 84-85 2.0598967283179945 2201 86-87 1.9309493967110711 2201 88-89 2.1551714939018254 2201 90-91 1.9403368660849765 2201 92-93 1.7443504447350229 2201 94-95 1.8283466364153576 2201 96-97 1.7418509994394462 2201 98-99 1.6964528624494548 2201 100-101 1.1129885568487978 2304 1 1.5415879417918106 2304 2 4.87141284064532 2304 3 5.7679349002142715 2304 4 4.188373691535887 2304 5 6.633773921581756 2304 6 6.4430069693348315 2304 7 7.300152897806093 2304 8 6.616285961661607 2304 9 6.069562585554639 2304 10-11 6.932951791474299 2304 12-13 6.716534329372159 2304 14-15 6.98291739570309 2304 16-17 5.651778741277504 2304 18-19 5.352658191176115 2304 20-21 5.050208790572835 2304 22-23 4.608675166841127 2304 24-25 4.860807836483524 2304 26-27 5.8823434085397075 2304 28-29 5.111265020224746 2304 30-31 5.081220742020527 2304 32-33 5.249853041590896 2304 34-35 4.898441210504277 2304 36-37 4.990591561263884 2304 38-39 4.767432025258461 2304 40-41 4.232317412875457 2304 42-43 3.5012361487704204 2304 44-45 3.3483834689811793 2304 46-47 4.159529193011778 2304 48-49 3.894949487581247 2304 50-51 3.51431028742822 2304 52-53 4.2255663211129075 2304 54-55 3.872239105970017 2304 56-57 4.511523129041063 2304 58-59 3.7371039803109447 2304 60-61 3.7602627210490205 2304 62-63 3.3692084437534007 2304 64-65 3.3341067070475034 2304 66-67 3.1046394817223515 2304 68-69 3.3800005120744245 2304 70-71 3.4132102424682635 2304 72-73 2.9567768654345237 2304 74-75 2.408951914157168 2304 76-77 2.558803891266976 2304 78-79 2.9047772405443624 2304 80-81 2.6627919934626725 2304 82-83 2.826327479302364 2304 84-85 2.3597783643786947 2304 86-87 2.178259734310327 2304 88-89 2.246024039539712 2304 90-91 2.192151900293055 2304 92-93 1.985524470446201 2304 94-95 2.299223143974384 2304 96-97 2.1691614454636827 2304 98-99 1.968569932517294 2304 100-101 1.4332853521677293 1102 1 7.113581111719444 1102 2 7.62865224897962 1102 3 6.265127520252054 1102 4 6.342530117613418 1102 5 6.6682207572089 1102 6 5.818358888156233 1102 7 6.86681882493172 1102 8 6.537004861290274 1102 9 4.919845870858893 1102 10-11 6.291432765959311 1102 12-13 6.090466288309564 1102 14-15 6.2454919763627785 1102 16-17 5.486008110753033 1102 18-19 5.450097735750315 1102 20-21 5.6086887379175145 1102 22-23 5.381414944535084 1102 24-25 1.9298617005443752 1102 26-27 5.398062353362551 1102 28-29 4.987358328939582 1102 30-31 4.944600559616838 1102 32-33 5.05448848483482 1102 34-35 5.1074235527293546 1102 36-37 4.928412388547365 1102 38-39 3.9222104464369387 1102 40-41 3.9251318677802978 1102 42-43 4.041575852838964 1102 44-45 -3.6002369582927685 1102 46-47 1.4526891779251443 1102 48-49 -0.7285623709593416 1102 50-51 2.6643756957247184 1102 52-53 -0.36210240722983755 1102 54-55 2.74021077497445 1102 56-57 -0.635546439584644 1102 58-59 2.968462451465964 1102 60-61 4.0477953738402555 1102 62-63 3.5772187825371518 1102 64-65 3.197134256594701 1102 66-67 3.223786527954349 1102 68-69 3.4948234618406886 1102 70-71 3.2917980641994298 1102 72-73 2.88072398496325 1102 74-75 2.7475955630197966 1102 76-77 2.9963034701394413 1102 78-79 -1.3967241510253672 1102 80-81 1.6828560294996855 1102 82-83 2.8750361173882375 1102 84-85 2.599596116814869 1102 86-87 2.668006203207284 1102 88-89 2.4419914044152904 1102 90-91 2.290754290982729 1102 92-93 2.3045706925829634 1102 94-95 2.5805095920491503 1102 96-97 2.523849026350984 1102 98-99 2.4933430783030204 1102 100-101 2.0596011805646235 2202 1 -20.759627863679313 2202 2 -7.799299535214113 2202 3 -0.7232015446925111 2202 4 1.7521640626166182 2202 5 1.2579667031431256 2202 6 1.7817504067484826 2202 7 3.927304532154224 2202 8 2.8693362436699594 2202 9 3.1813742251040757 2202 10-11 2.1983328278346868 2202 12-13 1.3560094037345962 2202 14-15 2.659942857725426 2202 16-17 1.5968288648085434 2202 18-19 1.6803921280777665 2202 20-21 1.057236853248586 2202 22-23 1.349480637965744 2202 24-25 2.40935766744105 2202 26-27 3.6902978524062284 2202 28-29 -1.1162629977522691 2202 30-31 0.7149736677556149 2202 32-33 1.4781482523250915 2202 34-35 1.450008916890507 2202 36-37 2.914261875617864 2202 38-39 2.451302081985215 2202 40-41 1.7713859319658773 2202 42-43 0.5092072530728728 2202 44-45 0.6197449923139153 2202 46-47 -1.3984271810271949 2202 48-49 0.7942002759043127 2202 50-51 1.8312517158371087 2202 52-53 1.737184029124549 2202 54-55 -0.5562878270469049 2202 56-57 1.1755784416883692 2202 58-59 2.1117371459563117 2202 60-61 2.1477877117029216 2202 62-63 -2.4029302279628695 2202 64-65 -0.16463156969452797 2202 66-67 0.46543899978876624 2202 68-69 1.4789595395925872 2202 70-71 -2.5893353502369045 2202 72-73 -4.724093195355753 2202 74-75 -1.4198773448191524 2202 76-77 -0.2647759081576275 2202 78-79 1.6504936799247574 2202 80-81 1.6793364158738022 2202 82-83 -0.7561325356513926 2202 84-85 -0.3323134143056983 2202 86-87 0.6882697102542821 2202 88-89 1.2090503670920043 2202 90-91 1.4699463836570033 2202 92-93 1.4977851290616755 2202 94-95 1.7804492707887434 2202 96-97 1.2185271298418847 2202 98-99 1.3615048918008128 2202 100-101 1.1051236280970578 2305 1 5.712524827184268 2305 2 7.134545327682691 2305 3 6.805705327534138 2305 4 7.826930816513535 2305 5 7.929125253603928 2305 6 6.278372604950782 2305 7 2.4939218947665793 2305 8 6.078041727797842 2305 9 6.47150251046077 2305 10-11 4.736541099537948 2305 12-13 5.239272573606023 2305 14-15 5.62109458147102 2305 16-17 5.167240032176842 2305 18-19 4.443874890621853 2305 20-21 5.2618036412790765 2305 22-23 5.267659611682891 2305 24-25 5.30029022518368 2305 26-27 6.140836167345359 2305 28-29 5.159888299323622 2305 30-31 5.497830790295154 2305 32-33 5.431687477492922 2305 34-35 5.242974555757268 2305 36-37 4.703625711094027 2305 38-39 4.688793548591196 2305 40-41 4.1659487377458255 2305 42-43 3.935596062948857 2305 44-45 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30-31 -25.870754941870054 1209 32-33 -25.570815651418222 1209 34-35 -22.791606330607095 1209 36-37 -25.98129293721636 1209 38-39 -26.27162572550142 1209 40-41 -22.936270382308084 1209 42-43 -23.737322810001306 1209 44-45 -26.31407853709785 1209 46-47 -19.982665491736096 1209 48-49 -19.76309353322773 1209 50-51 -21.932537191334152 1209 52-53 -24.369266505634812 1209 54-55 -21.772757386898817 1209 56-57 -25.20610605029024 1209 58-59 -25.477333709658662 1209 60-61 -25.221196245516328 1209 62-63 -25.34978851673292 1209 64-65 -25.205406970764045 1209 66-67 -13.047036147612854 1209 68-69 -24.833373348107948 1209 70-71 -24.651835350236905 1209 72-73 -15.051176528689087 1209 74-75 -16.05753160232635 1209 76-77 -13.320418304976494 1209 78-79 -13.715396557289012 1209 80-81 -23.46137214027593 1209 82-83 -15.380160713356041 1209 84-85 -12.296760486841006 1209 86-87 -12.985020602374265 1209 88-89 -9.825178516769231 1209 90-91 -12.51402454737275 1209 92-93 -16.445268141197474 1209 94-95 -21.607919713168474 1209 96-97 -17.475683071721257 1209 98-99 -9.154635585369075 1209 100-101 -4.22737242976665 1212 1 -20.759627863679313 1212 2 -20.585730016497536 1212 3 -21.505621330788767 1212 4 -23.522568557704236 1212 5 -23.66349051610821 1212 6 -24.746658684160607 1212 7 -24.29863129672278 1212 8 -23.93447391675785 1212 9 -24.85171401019004 1212 10-11 -24.54865915077494 1212 12-13 -24.833161719260055 1212 14-15 -25.161915431044626 1212 16-17 -26.182415787597876 1212 18-19 -20.68727427493477 1212 20-21 -26.590824644077617 1212 22-23 -26.775519362034256 1212 24-25 -22.782626423038167 1212 26-27 -25.45960188021409 1212 28-29 -20.321361373700135 1212 30-31 -15.445513784313784 1212 32-33 -25.570815651418222 1212 34-35 -21.85636434858055 1212 36-37 -25.98129293721636 1212 38-39 -26.27162572550142 1212 40-41 -20.78483580402463 1212 42-43 -17.20862712198086 1212 44-45 -13.96681165606891 1212 46-47 -17.22796188880098 1212 48-49 -20.119079187444896 1212 50-51 -12.19473935144628 1212 52-53 -5.029234351293976 1212 54-55 -3.599086845221848 1212 56-57 -3.9673600695828455 1212 58-59 -2.565758146957698 1212 60-61 -13.971196245516328 1212 62-63 -11.611846394546427 1212 64-65 -15.959426263368547 1212 66-67 -12.147613276807345 1212 68-69 -7.685463380262288 1212 70-71 -14.96534017338803 1212 72-73 -11.297746732333245 1212 74-75 -8.004575876380269 1212 76-77 -18.59252235312556 1212 78-79 -9.339046487209039 1212 80-81 -7.482272461819338 1212 82-83 -4.793813941157179 1212 84-85 -3.885601281630354 1212 86-87 -2.899465321642136 1212 88-89 -2.6970104897266083 1212 90-91 -2.793886860836352 1212 92-93 -2.730019563408703 1212 94-95 -2.403739648859794 1212 96-97 -12.11246269081599 1212 98-99 -9.192565340501401 1212 100-101 -5.393923670594415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 320.0 3 648.0 4 319.0 5 1786.0 6 2298.0 7 2271.0 8 4748.0 9 8813.0 10 11461.0 11 14184.0 12 18828.0 13 23494.0 14 26967.0 15 29948.0 16 32122.0 17 35687.0 18 40713.0 19 45333.0 20 49674.0 21 56241.0 22 62244.0 23 66870.0 24 70490.0 25 73222.0 26 76731.0 27 79972.0 28 81641.0 29 82611.0 30 86249.0 31 90460.0 32 96844.0 33 110186.0 34 122251.0 35 131524.0 36 133048.0 37 116962.0 38 39546.0 39 87.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 30.993505455620813 24.108354549802783 21.752263871620055 23.14587612295635 2 26.93226134014244 31.132043156086365 20.486019789839634 21.44967571393156 3 19.254573433312597 27.175009685658186 27.955642260715603 25.614774620313614 4 22.687263069218886 27.58687461974072 27.045209903121638 22.680652407918753 5 22.649681876736558 27.31465484607866 26.65205221809645 23.38361105908833 6 22.371348024131706 28.711222949389658 25.667978045786406 23.24945098069223 7 22.955772997714817 27.84169411732372 27.012935257293723 22.18959762766774 8 22.689583222086526 27.543503961534732 26.807753457550803 22.959159358827943 9 22.10602624531187 26.57883578488364 28.1556335504485 23.159504419355986 10-11 23.167055684859896 26.734170164514907 26.70286635677831 23.39590779384688 12-13 23.005071117327525 27.17339557039474 26.61804738056076 23.203485931716976 14-15 23.191526656577494 27.231922802743615 26.48391528442931 23.092635256249586 16-17 23.290900907638875 27.152964072661153 26.378778801052192 23.177356218647784 18-19 23.25086285642032 27.306227883915973 26.37179534818071 23.071113911483003 20-21 23.329616112282174 27.266404465369703 26.2952314611619 23.108747961186225 22-23 23.20659309511267 27.06599780194509 26.489797228097668 23.23761187484457 24-25 23.05307013411478 27.2341614611728 26.671106469092777 23.041661935619643 26-27 23.095638678004203 27.21541661007098 26.540029427951577 23.14891528397324 28-29 23.241523562144888 27.133229493995643 26.54705528930197 23.0781916545575 30-31 23.176050769137372 27.1141634897075 26.568462597419607 23.14132314373552 32-33 23.16754881375685 27.20874518528295 26.518247233265946 23.105458767694262 34-35 23.20738464988685 27.03683359651798 26.55389793160937 23.201883821985806 36-37 23.161619429780277 27.13065604951063 26.588612544427768 23.119111976281324 38-39 23.15252742029949 27.110503465194014 26.69353405626958 23.04343505823692 40-41 23.314419111794173 26.996841741276747 26.48209646672757 23.20664268020151 42-43 23.163405757503753 27.30378791652984 26.525588253475814 23.007218072490595 44-45 23.142114522218247 27.01965085573596 26.7048400390191 23.13339458302669 46-47 23.287439360212623 27.008896244821123 26.50821799387537 23.19544640109088 48-49 23.107652468956378 27.085274749627864 26.815110999779172 22.991961781636586 50-51 23.42574583935638 27.011653805793085 26.42762018349397 23.134980171356563 52-53 23.20333420227951 27.10524798315467 26.604288554969084 23.08712925959674 54-55 23.18310186284912 27.100178947535113 26.678358961365277 23.038360228250486 56-57 23.26740135214721 27.145050798572512 26.568153662547118 23.019394186733162 58-59 23.420451240944203 27.120315114274756 26.427249145520676 23.031984499260364 60-61 23.426738783262465 27.067408862980656 26.451587463279026 23.05426489047785 62-63 23.583345337333274 27.1086069723407 26.346666123877107 22.961381566448924 64-65 23.759046696667642 27.0517856847503 26.232932798142915 22.956234820439136 66-67 23.794834172458973 27.146710190928257 26.223350216725567 22.8351054198872 68-69 23.85972618211909 27.172365650013532 26.181648476196827 22.786259691670555 70-71 24.175922047597005 27.02652436444812 25.980816286222247 22.816737301732623 72-73 24.117491080961265 27.25739266702697 26.03473704905192 22.590379202959845 74-75 24.37320359145335 27.155553881541266 25.779237056442135 22.692005470563252 76-77 24.63557193248392 27.147617019657353 25.641270901998887 22.57554014585984 78-79 24.682237371260936 27.234725964565065 25.70359388761232 22.37944277656168 80-81 25.059804908764015 27.141255109501394 25.421522481806143 22.37741749992845 82-83 25.17019414217694 27.168640629290053 25.41571071389367 22.24545451463934 84-85 25.557856470511865 27.144587620500506 25.163036774968354 22.13451913401928 86-87 25.779787298150392 27.2323719913473 24.96620687935466 22.021633831147646 88-89 26.101849944955873 27.140611450788683 24.87560858236505 21.881930021890398 90-91 26.519019757359924 27.11611422658735 24.561957576424977 21.80290843962775 92-93 26.859803989857454 27.177278251440658 24.381841355915288 21.5810764027866 94-95 27.295670480482414 27.08459770702005 24.12259523616568 21.49713657633186 96-97 27.63500923340357 27.164798286846903 23.96802523901131 21.232167240738214 98-99 28.009383322323455 27.168374236060046 23.723217958145852 21.099024483470647 100-101 28.912361093412326 26.907399075733185 23.178786074102582 21.001453756751907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 134.0 1 121.5 2 137.5 3 158.5 4 139.5 5 152.0 6 201.5 7 238.5 8 264.0 9 351.5 10 501.5 11 666.5 12 889.0 13 1170.0 14 1516.0 15 1941.0 16 2498.0 17 3153.0 18 3887.0 19 4848.0 20 6111.5 21 7805.0 22 9723.5 23 11853.0 24 14360.0 25 17186.0 26 20207.5 27 23523.0 28 27026.0 29 30615.5 30 34340.0 31 38137.0 32 41988.0 33 45654.5 34 48914.0 35 51801.0 36 54514.0 37 57248.5 38 58929.5 39 59425.5 40 59246.5 41 58343.5 42 57069.5 43 55306.0 44 53208.0 45 51138.0 46 49273.5 47 47449.5 48 45327.0 49 43375.5 50 41500.0 51 39228.0 52 37489.0 53 36197.5 54 34874.0 55 33880.5 56 33218.0 57 32707.5 58 31987.0 59 31191.5 60 30201.5 61 29064.0 62 28106.5 63 26783.5 64 25503.0 65 24378.0 66 23108.0 67 21679.0 68 20132.0 69 18684.0 70 17087.0 71 15539.0 72 14004.0 73 12328.0 74 10848.0 75 9399.0 76 8053.0 77 6859.0 78 5714.0 79 4726.0 80 3875.0 81 3200.5 82 2689.0 83 2206.5 84 1781.0 85 1456.0 86 1184.0 87 939.5 88 739.5 89 575.5 90 458.0 91 345.0 92 253.5 93 210.0 94 152.0 95 102.0 96 69.0 97 43.5 98 27.5 99 10.5 100 3.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.302622544300297 2 10.607989545322202 3 9.308939777132261 4 11.28470987802011 5 8.827310458362678 6 5.042939225957329 7 9.358867299185745 8 9.610269499629695 9 5.14004358537736 10-11 6.077923264201188 12-13 5.101767548460058 14-15 7.028985469637891 16-17 2.503512312946954 18-19 2.9274810527129795 20-21 2.917801756597621 22-23 2.9561037433704604 24-25 3.3266417305854854 26-27 4.094368206652193 28-29 1.6544849394823418 30-31 2.1116175946248505 32-33 2.8709622673530575 34-35 1.8771346999911251 36-37 2.8729863560849553 38-39 1.2837393534230195 40-41 1.0896863337161802 42-43 0.5121722987368129 44-45 1.1994023229272681 46-47 1.2701675789770879 48-49 1.171765726780199 50-51 1.4806468572389457 52-53 2.346048589547502 54-55 1.6806683437193306 56-57 1.6968351037189775 58-59 1.4823076479933237 60-61 1.2861007902769006 62-63 0.5587003897149304 64-65 1.157337607101541 66-67 0.5352936200204174 68-69 0.7468627922148358 70-71 0.9361929382139129 72-73 0.08083379999823541 74-75 0.1009708878950671 76-77 0.4347119799584076 78-79 0.8228180193720862 80-81 0.26614171838905376 82-83 0.4776589908723978 84-85 0.06386259447693654 86-87 0.09575496693209909 88-89 0.07886161097741169 90-91 0.08495982702864294 92-93 0.15647762888904 94-95 0.41641733180471385 96-97 0.342070995690767 98-99 0.06482273913181125 100-101 0.2173559899792038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1926793.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.09580738517255 #Duplication Level Percentage of deduplicated Percentage of total 1 99.28037270432206 98.38268690635641 2 0.581425193503585 1.1523359796863588 3 0.10132763426883173 0.3012343118489808 4 0.028557600236827692 0.11319753809805738 5 0.002543723116837241 0.012603614801365702 6 0.0021241667866416752 0.012629761364581462 7 0.0036489777652178542 0.025311887844236415 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.037994221486169E-4 0.0 5.189971107430845E-5 2 1.5569913322292534E-4 0.0 5.189971107430845E-5 3 2.075988442972338E-4 0.0 5.189971107430845E-5 4 2.075988442972338E-4 0.0 5.189971107430845E-5 5 2.075988442972338E-4 0.0 5.189971107430845E-5 6 2.5949855537154225E-4 0.0 5.189971107430845E-5 7 3.113982664458507E-4 0.0 5.189971107430845E-5 8 3.6329797752015917E-4 0.0 5.189971107430845E-5 9 5.189971107430845E-4 0.0 5.189971107430845E-5 10-11 5.189971107430845E-4 0.0 1.037994221486169E-4 12-13 5.70896821817393E-4 0.0 1.037994221486169E-4 14-15 6.487463884288557E-4 0.0 1.037994221486169E-4 16-17 7.00646099503164E-4 0.0 1.037994221486169E-4 18-19 7.525458105774725E-4 0.0 1.037994221486169E-4 20-21 9.08244943800398E-4 0.0 1.5569913322292534E-4 22-23 0.0010639440770233233 2.5949855537154225E-5 1.5569913322292534E-4 24-25 0.0012196432102462487 7.784956661146267E-5 2.5949855537154225E-4 26-27 0.001608891043303562 1.037994221486169E-4 2.5949855537154225E-4 28-29 0.0025690356981782686 1.037994221486169E-4 2.854484109086965E-4 30-31 0.0043336258747047555 1.5569913322292534E-4 3.113982664458507E-4 32-33 0.007291909405940338 1.5569913322292534E-4 3.113982664458507E-4 34-35 0.012352131235685411 1.5569913322292534E-4 3.113982664458507E-4 36-37 0.01909909367534551 1.5569913322292534E-4 3.113982664458507E-4 38-39 0.030491080256156215 1.5569913322292534E-4 3.113982664458507E-4 40-41 0.04803318259927247 1.5569913322292534E-4 3.113982664458507E-4 42-43 0.07413873726964962 1.5569913322292534E-4 3.113982664458507E-4 44-45 0.11176602779852324 1.5569913322292534E-4 3.113982664458507E-4 46-47 0.16742846792571905 1.5569913322292534E-4 3.113982664458507E-4 48-49 0.23775257643140701 1.5569913322292534E-4 3.6329797752015917E-4 50-51 0.32580043626897126 1.8164898876007958E-4 3.6329797752015917E-4 52-53 0.4416924910979021 2.075988442972338E-4 3.6329797752015917E-4 54-55 0.5947187892005005 2.075988442972338E-4 4.151976885944676E-4 56-57 0.7807014038352849 2.075988442972338E-4 4.151976885944676E-4 58-59 1.0188432280997493 2.5949855537154225E-4 4.151976885944676E-4 60-61 1.2960655348031678 2.5949855537154225E-4 4.151976885944676E-4 62-63 1.6123164242344663 2.5949855537154225E-4 4.151976885944676E-4 64-65 1.983814556104366 2.5949855537154225E-4 4.151976885944676E-4 66-67 2.41318086582212 2.5949855537154225E-4 4.151976885944676E-4 68-69 2.9009343504984706 2.5949855537154225E-4 4.151976885944676E-4 70-71 3.444298375590943 2.5949855537154225E-4 4.151976885944676E-4 72-73 4.017945882095274 2.5949855537154225E-4 4.67097399668776E-4 74-75 4.639574671487804 2.854484109086965E-4 4.67097399668776E-4 76-77 5.317618446818107 3.113982664458507E-4 4.67097399668776E-4 78-79 6.062301451167821 3.113982664458507E-4 4.67097399668776E-4 80-81 6.855225236961106 3.373481219830049E-4 4.67097399668776E-4 82-83 7.7045899585477 3.6329797752015917E-4 4.67097399668776E-4 84-85 8.603596753776873 3.6329797752015917E-4 4.67097399668776E-4 86-87 9.558188139566628 3.6329797752015917E-4 4.67097399668776E-4 88-89 10.558087973124254 3.6329797752015917E-4 4.67097399668776E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGGGT 385 0.00417052 6.471865 88-89 GTAGGGA 5345 0.0 6.3344665 94-95 TAGGGAA 4910 0.0 5.5998363 94-95 ACCTCCA 1365 0.0015037959 5.4751425 4 GAGTGTA 920 1.2315213E-6 5.4338336 92-93 GTGTAGG 8365 0.0 5.3514533 92-93 GTCGTGT 11065 0.0 5.318875 94-95 TGTAGGG 7795 0.0 5.276354 94-95 TCGTGTA 9345 0.0 5.2522564 94-95 >>END_MODULE