FastQCFastQC Report
Thu 11 Sep 2014
NYCLib12_GGCTAC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib12_GGCTAC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926793
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGT3850.004170526.47186588-89
GTAGGGA53450.06.334466594-95
TAGGGAA49100.05.599836394-95
ACCTCCA13650.00150379595.47514254
GAGTGTA9201.2315213E-65.433833692-93
GTGTAGG83650.05.351453392-93
GTCGTGT110650.05.31887594-95
TGTAGGG77950.05.27635494-95
TCGTGTA93450.05.252256494-95
Produced by FastQC (version 0.11.2)