##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	NYCLib12_GGCTAC_L007_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1926793
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21822582913681	33.0	33.0	33.0	27.0	33.0
2	31.61391753032111	33.0	33.0	33.0	27.0	33.0
3	31.775100906013257	33.0	33.0	33.0	27.0	33.0
4	35.599172303407784	37.0	37.0	37.0	33.0	37.0
5	35.107843966632636	37.0	37.0	37.0	33.0	37.0
6	34.88427454324362	37.0	33.0	37.0	27.0	37.0
7	34.67860169722435	37.0	33.0	37.0	27.0	37.0
8	34.71437772505921	37.0	33.0	37.0	27.0	37.0
9	34.63094115455059	37.0	33.0	37.0	27.0	37.0
10-11	34.476364093081095	37.0	33.0	37.0	27.0	37.0
12-13	34.46675460207713	37.0	33.0	37.0	27.0	37.0
14-15	35.34451988355781	37.0	33.0	40.0	27.0	40.0
16-17	35.24899509184432	37.0	33.0	40.0	27.0	40.0
18-19	35.45783018725935	37.0	33.0	40.0	27.0	40.0
20-21	35.12513772885826	37.0	33.0	40.0	27.0	40.0
22-23	35.142431750582446	37.0	33.0	40.0	27.0	40.0
24-25	34.97450193144775	37.0	33.0	40.0	27.0	40.0
26-27	34.56573747153949	37.0	33.0	40.0	27.0	40.0
28-29	34.174645641747716	37.0	33.0	38.5	24.5	40.0
30-31	34.0536313449343	37.0	33.0	37.0	22.0	40.0
32-33	34.05010060758992	37.0	33.0	37.0	24.5	40.0
34-35	33.87511969370867	37.0	33.0	37.0	22.0	40.0
36-37	33.55621413405592	37.0	33.0	37.0	22.0	40.0
38-39	33.44818436645763	37.0	33.0	37.0	22.0	40.0
40-41	33.150261340995115	37.0	33.0	37.0	22.0	40.0
42-43	32.7515036643791	37.0	33.0	37.0	22.0	40.0
44-45	32.6112358722499	37.0	33.0	37.0	22.0	40.0
46-47	32.462286815449296	37.0	33.0	37.0	22.0	40.0
48-49	32.478121157799514	37.0	33.0	37.0	22.0	40.0
50-51	32.79213802416762	37.0	33.0	37.0	22.0	40.0
52-53	32.64209206697346	37.0	33.0	37.0	22.0	40.0
54-55	32.27894433911686	37.0	33.0	37.0	18.5	40.0
56-57	32.317119950093236	37.0	33.0	37.0	22.0	40.0
58-59	32.0191377589601	35.0	27.0	37.0	22.0	40.0
60-61	31.904288628825203	33.0	27.0	37.0	22.0	40.0
62-63	31.890028923708982	33.0	27.0	37.0	22.0	40.0
64-65	31.54892300314564	33.0	27.0	37.0	18.5	40.0
66-67	31.188307202693803	33.0	27.0	37.0	15.0	40.0
68-69	31.017731536288537	33.0	27.0	37.0	15.0	40.0
70-71	30.85375439914926	33.0	27.0	37.0	15.0	37.0
72-73	30.632222039419908	33.0	27.0	37.0	15.0	37.0
74-75	30.282121379930278	33.0	27.0	37.0	15.0	37.0
76-77	29.159555281755747	33.0	27.0	35.0	15.0	37.0
78-79	30.068840814763185	33.0	27.0	37.0	15.0	37.0
80-81	29.800894024422966	33.0	27.0	37.0	15.0	37.0
82-83	29.769882909061845	33.0	27.0	37.0	15.0	37.0
84-85	29.63679284697422	33.0	27.0	37.0	15.0	37.0
86-87	29.428397342111996	33.0	27.0	37.0	15.0	37.0
88-89	29.28402583982815	33.0	27.0	35.0	15.0	37.0
90-91	29.18243163640308	33.0	27.0	33.0	15.0	37.0
92-93	28.962779343707393	33.0	27.0	33.0	15.0	37.0
94-95	28.858377625411762	33.0	27.0	33.0	15.0	37.0
96-97	28.700426823223875	33.0	27.0	33.0	15.0	37.0
98-99	28.41570734375722	33.0	27.0	33.0	15.0	37.0
100-101	27.563451289266673	33.0	24.5	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	1.1185421673364573
2306	2	0.5313724520276182
2306	3	0.4473640389245226
2306	4	0.36256970064189886
2306	5	0.7191880467602516
2306	6	0.7263022141867452
2306	7	0.809884789992708
2306	8	0.7542231644222781
2306	9	0.9894510169180322
2306	10-11	1.2430790292731047
2306	12-13	1.397878350170707
2306	14-15	1.4902770553343032
2306	16-17	1.472819592851522
2306	18-19	1.2495927093223145
2306	20-21	1.554855238253559
2306	22-23	1.162031319900592
2306	24-25	1.1886146211384485
2306	26-27	1.2893543031180883
2306	28-29	1.6099530517237355
2306	30-31	1.2485372206112118
2306	32-33	1.053152107226964
2306	34-35	0.9850912243617245
2306	36-37	1.0897560213381823
2306	38-39	1.0235323628864208
2306	40-41	1.1038380191436516
2306	42-43	0.9797128746792936
2306	44-45	0.9327389216027058
2306	46-47	0.7283711152981311
2306	48-49	0.6449233235675678
2306	50-51	0.9100963442382337
2306	52-53	0.8783051789140472
2306	54-55	1.4459117816028808
2306	56-57	0.8317219122214254
2306	58-59	0.8308705341430986
2306	60-61	0.5851600127111034
2306	62-63	0.5240675025429731
2306	64-65	0.5678099527835236
2306	66-67	0.7149725758211929
2306	68-69	0.5613248399312099
2306	70-71	0.531398101207234
2306	72-73	0.22335515852934762
2306	74-75	0.48621879243994925
2306	76-77	0.38167605097426005
2306	78-79	0.3409580433442265
2306	80-81	0.8151806088068767
2306	82-83	0.7966809814216909
2306	84-85	0.4517562476334014
2306	86-87	0.3961854142412591
2306	88-89	0.23059637376852393
2306	90-91	-0.13173590692542803
2306	92-93	-0.3283079146897876
2306	94-95	-0.27373176073270145
2306	96-97	-0.31516265715516667
2306	98-99	-0.301006052198467
2306	100-101	-0.34583637660529476
2307	1	0.6462539508956375
2307	2	0.43411482771839616
2307	3	0.34063520480716747
2307	4	0.4585121690099214
2307	5	0.6588790323983744
2307	6	0.659562370978243
2307	7	0.8359356714944823
2307	8	0.932943334750135
2307	9	1.1345378594399307
2307	10-11	1.290106778476492
2307	12-13	1.4599502539494225
2307	14-15	1.377181144660561
2307	16-17	1.446939179430153
2307	18-19	1.2644927282849494
2307	20-21	1.5416786507685458
2307	22-23	1.3376522484567772
2307	24-25	1.31236727333971
2307	26-27	1.4931471404586034
2307	28-29	1.7185919272833416
2307	30-31	1.7443250855991863
2307	32-33	1.3479413928638024
2307	34-35	1.2729222165511729
2307	36-37	1.4999446156132237
2307	38-39	1.1000412255145804
2307	40-41	1.2945241356394632
2307	42-43	1.3589746785951888
2307	44-45	1.3130688617163173
2307	46-47	1.1371387346131527
2307	48-49	1.246961391348151
2307	50-51	1.1646982079247508
2307	52-53	1.3848018796922617
2307	54-55	1.6963806707298232
2307	56-57	1.3181305272265575
2307	58-59	1.3451487644643088
2307	60-61	1.149843314649285
2307	62-63	0.9506811977414777
2307	64-65	1.1032678841798607
2307	66-67	1.2502576749217624
2307	68-69	1.0397324609876009
2307	70-71	1.0013861806703055
2307	72-73	0.8858094456099899
2307	74-75	0.9033252439271422
2307	76-77	0.9112261342680092
2307	78-79	1.1358900786744108
2307	80-81	1.7364572916268415
2307	82-83	1.4377109118990852
2307	84-85	1.2067037759114534
2307	86-87	1.1350172277102146
2307	88-89	1.0074989842373014
2307	90-91	0.7703430210186681
2307	92-93	0.8176143926278847
2307	94-95	0.6772229991094854
2307	96-97	0.6054706329863997
2307	98-99	0.7082018329326232
2307	100-101	0.8162037651628431
1101	1	1.017895171475594
1101	2	0.5303280822336269
1101	3	0.3608355319184078
1101	4	0.31748974168881716
1101	5	0.5576475330758868
1101	6	0.5293307891061403
1101	7	0.7131640916492046
1101	8	0.614440408936737
1101	9	0.9144667929458805
1101	10-11	0.9240083786638706
1101	12-13	1.1878269570385882
1101	14-15	1.2135111800360932
1101	16-17	1.273090612337107
1101	18-19	0.8755940240454549
1101	20-21	1.2277118430963938
1101	22-23	0.9444200249795358
1101	24-25	1.0181490349753446
1101	26-27	0.9818764334245031
1101	28-29	1.1448590974355497
1101	30-31	1.0795023625759654
1101	32-33	0.7229673993483132
1101	34-35	0.8866458048688628
1101	36-37	0.925602227325335
1101	38-39	0.730106449777729
1101	40-41	0.7791717528079047
1101	42-43	0.8373201695405683
1101	44-45	0.652577057282393
1101	46-47	0.5761291159051254
1101	48-49	0.6088033685658019
1101	50-51	0.479083971673127
1101	52-53	0.6940066483754848
1101	54-55	1.0698207011188217
1101	56-57	0.8041085019153655
1101	58-59	0.8012640802367166
1101	60-61	0.4564452536927668
1101	62-63	0.25269886470143277
1101	64-65	0.3853926038970741
1101	66-67	0.49809947227520723
1101	68-69	0.403534991569547
1101	70-71	0.5689263632056232
1101	72-73	0.4343417402946663
1101	74-75	0.5040671502058132
1101	76-77	0.5504107420533124
1101	78-79	0.5004488107123173
1101	80-81	1.1430538280328975
1101	82-83	0.8974337465320694
1101	84-85	0.8094381983628018
1101	86-87	0.6848343266214307
1101	88-89	0.42899438394186973
1101	90-91	0.21632461648125556
1101	92-93	0.2312409851475472
1101	94-95	0.26110515315780347
1101	96-97	0.3734521036178613
1101	98-99	0.48401737950839063
1101	100-101	0.3599377795640599
2201	1	1.331147246717645
2201	2	0.7901520922760774
2201	3	0.3402531616792821
2201	4	0.21031456352621092
2201	5	0.31438543109962325
2201	6	0.4336499760985575
2201	7	0.5570766401126903
2201	8	0.507891480607114
2201	9	0.6645503303507638
2201	10-11	0.792067700326669
2201	12-13	1.055454472283607
2201	14-15	1.0649480177860156
2201	16-17	0.8542441415117636
2201	18-19	0.7538070141119206
2201	20-21	1.0500244986763931
2201	22-23	0.7424989734412222
2201	24-25	0.7468965689938187
2201	26-27	0.9143026448572229
2201	28-29	1.1866904810013637
2201	30-31	1.2532434347965236
2201	32-33	0.8946435313418988
2201	34-35	0.7621060465659255
2201	36-37	0.9735495257773081
2201	38-39	0.6649036806924045
2201	40-41	1.067176107082851
2201	42-43	0.98428828946615
2201	44-45	0.7928388583443535
2201	46-47	0.6701310123389561
2201	48-49	0.8614964393401721
2201	50-51	0.6592077675948431
2201	52-53	0.8844108784671434
2201	54-55	1.2250485819522652
2201	56-57	0.8282669804566964
2201	58-59	0.973556665506063
2201	60-61	0.7826653040173177
2201	62-63	0.5912828720042178
2201	64-65	0.7146466395733846
2201	66-67	0.6506900862189937
2201	68-69	0.571899241602388
2201	70-71	0.7494067369681439
2201	72-73	0.5493556238969681
2201	74-75	0.6794745354064453
2201	76-77	0.5626193014934309
2201	78-79	0.6898919395923464
2201	80-81	1.1467151298914935
2201	82-83	1.125666919190639
2201	84-85	0.9706288141030903
2201	86-87	0.9927436851686906
2201	88-89	0.8966979979451644
2201	90-91	0.6409914773948451
2201	92-93	0.8022603548218967
2201	94-95	0.7154045786135832
2201	96-97	0.5072587875085048
2201	98-99	0.5630385434404452
2201	100-101	0.748060815340299
2304	1	-1.9843322266260728
2304	2	-0.3540260282852721
2304	3	-0.13908826497663895
2304	4	-0.03876436624693014
2304	5	0.479614262025315
2304	6	0.45237641625338654
2304	7	0.5633164978591125
2304	8	0.733411107336039
2304	9	1.018788843627405
2304	10-11	0.9831486612611542
2304	12-13	1.1834051678298678
2304	14-15	1.3290729016809308
2304	16-17	1.2057512487693742
2304	18-19	0.9428927746589579
2304	20-21	1.1992353455116458
2304	22-23	0.9799057731332965
2304	24-25	1.1186018048362243
2304	26-27	1.0593369613642096
2304	28-29	1.501911902886583
2304	30-31	1.474122652980114
2304	32-33	0.8633403353348328
2304	34-35	0.9939853406470007
2304	36-37	1.2398486919891951
2304	38-39	0.9981910538803547
2304	40-41	0.9740061581510204
2304	42-43	1.08447080485346
2304	44-45	0.952240027165665
2304	46-47	0.9085289970799408
2304	48-49	0.9530709146536083
2304	50-51	0.8234790252162085
2304	52-53	1.1412093055820662
2304	54-55	1.4687631857131649
2304	56-57	1.1436195965708151
2304	58-59	0.7407664208631637
2304	60-61	0.5085976685131932
2304	62-63	0.37638726719159976
2304	64-65	0.4643464830799964
2304	66-67	0.5456951279806788
2304	68-69	0.45314967078461166
2304	70-71	0.4188066769729879
2304	72-73	0.367925209243694
2304	74-75	0.4955447441968204
2304	76-77	0.4921873363629068
2304	78-79	0.42192646210679285
2304	80-81	1.1271003277128706
2304	82-83	0.9230645389008529
2304	84-85	0.63881349076172
2304	86-87	0.5381177332876952
2304	88-89	0.4094310664805967
2304	90-91	0.214481749866831
2304	92-93	0.2587935021461476
2304	94-95	0.1809435049894077
2304	96-97	0.09078343069995753
2304	98-99	0.43296000761140974
2304	100-101	0.4673479933632798
1102	1	1.4709976867407981
1102	2	-7.77101236064226E-4
1102	3	0.23498860291914525
1102	4	0.1744112969365048
1102	5	0.2527743455441538
1102	6	0.10454807851978387
1102	7	-5.074282672540182
1102	8	-1.2728398222259614
1102	9	0.25437892753244284
1102	10-11	0.7066761734204334
1102	12-13	0.8958402628321309
1102	14-15	0.7565938943750865
1102	16-17	0.5766780416017383
1102	18-19	0.8500690754911062
1102	20-21	1.1572034482848395
1102	22-23	0.6448063747088497
1102	24-25	0.9739337313981551
1102	26-27	1.0997560839234168
1102	28-29	1.0318016866201276
1102	30-31	1.2500507153579434
1102	32-33	1.0960263648293704
1102	34-35	1.319039388771401
1102	36-37	1.2998147470554215
1102	38-39	1.126919059724365
1102	40-41	1.2384921648019542
1102	42-43	1.367585603809232
1102	44-45	1.3355739356975889
1102	46-47	1.0528994052490788
1102	48-49	0.9050205913509188
1102	50-51	0.8222311318810398
1102	52-53	1.0729227975222955
1102	54-55	1.4033175423905249
1102	56-57	1.151795304948422
1102	58-59	1.1823112892289664
1102	60-61	1.0513977023677405
1102	62-63	0.6795619936230359
1102	64-65	0.5256536958237668
1102	66-67	0.8474316936013899
1102	68-69	0.867699565564358
1102	70-71	0.7115544910129259
1102	72-73	0.6195803431786864
1102	74-75	0.7292656381211629
1102	76-77	0.728043449614578
1102	78-79	0.7018475119921241
1102	80-81	1.2639150094103009
1102	82-83	1.1618984552390401
1102	84-85	0.8009538786592145
1102	86-87	0.7399974822710291
1102	88-89	0.6552370059812098
1102	90-91	0.3465809946310827
1102	92-93	0.41197661004272135
1102	94-95	0.43066901200641894
1102	96-97	0.3402641085468403
1102	98-99	0.29383524621610846
1102	100-101	0.516621846232411
2202	1	1.4899806319796518
2202	2	0.8981648190401152
2202	3	0.44371471114611083
2202	4	-0.19708137777107027
2202	5	0.039018062230120165
2202	6	0.5385139176756226
2202	7	0.7960347495698556
2202	8	-4.264199135987596
2202	9	-0.5758085020807044
2202	10-11	0.5132212560630904
2202	12-13	0.019505894387414457
2202	14-15	0.1984832238368739
2202	16-17	0.3200516933185682
2202	18-19	0.809295888128716
2202	20-21	1.2003911124605935
2202	22-23	-2.7457867524262554
2202	24-25	-2.8353913156227897
2202	26-27	-1.6219309181280153
2202	28-29	0.5340076231863051
2202	30-31	1.2980315976024457
2202	32-33	1.285432682912166
2202	34-35	-2.1291055156181287
2202	36-37	-0.35303567645837575
2202	38-39	0.34916599414659544
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2202	58-59	1.6554339029430416
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2202	64-65	1.1228154702880353
2202	66-67	1.3508525487826546
2202	68-69	1.2744031044598216
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2202	78-79	1.3339003377305438
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2305	1	1.3282728153907328
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2203	3	-3.137111959929939E-4
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1209	90-91	-5.581890317781838
1209	92-93	-3.0909025464860704
1209	94-95	-0.6677900309781748
1209	96-97	0.6116430675170399
1209	98-99	1.3395378269551834
1209	100-101	0.9782278936048847
1212	1	-28.602265357325162
1212	2	-16.242636159345974
1212	3	-5.772584487574591
1212	4	-1.2471430642404542
1212	5	-0.679700234793458
1212	6	-2.139058214655826
1212	7	-0.2538367190409758
1212	8	-1.2932046727243574
1212	9	-0.022105809369598717
1212	10-11	0.8163516831827877
1212	12-13	0.082213307080643
1212	14-15	-0.17558556839794193
1212	16-17	0.041975214992653775
1212	18-19	-0.645013674778582
1212	20-21	-0.21702800733581995
1212	22-23	-0.26048457470044184
1212	24-25	0.485426148311042
1212	26-27	-0.555831286957222
1212	28-29	0.6353475306780965
1212	30-31	-0.5771210065552594
1212	32-33	0.6277936626738381
1212	34-35	-0.8929941059855793
1212	36-37	-0.8162107886546721
1212	38-39	-0.4613936762284254
1212	40-41	-0.39618706109467183
1212	42-43	-0.07008504498870138
1212	44-45	1.3256066550646821
1212	46-47	-2.083081554235058
1212	48-49	-0.9008042209516987
1212	50-51	0.22049619700248968
1212	52-53	-0.5654927732107353
1212	54-55	0.13526647444741968
1212	56-57	-1.2966197529146157
1212	58-59	0.005704506653682273
1212	60-61	0.7727089283965007
1212	62-63	-4.088414968952229
1212	64-65	-3.6068656085159
1212	66-67	-1.0191330075562206
1212	68-69	0.16909978757621502
1212	70-71	-0.34439810415270955
1212	72-73	-0.974586934150981
1212	74-75	-0.044748847413728754
1212	76-77	1.532875771550895
1212	78-79	-0.5302481095758864
1212	80-81	1.1282902337909242
1212	82-83	0.9130106877099671
1212	84-85	-3.0849538447282647
1212	86-87	-1.3952406849923875
1212	88-89	-0.47741986052220753
1212	90-91	0.538053618239271
1212	92-93	-2.085757851952309
1212	94-95	-1.7823130749224383
1212	96-97	0.30423107971919805
1212	98-99	0.10119171433254692
1212	100-101	0.6723700322807851
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	1.0
11	10.0
12	44.0
13	237.0
14	762.0
15	1768.0
16	3208.0
17	5200.0
18	7829.0
19	11289.0
20	15059.0
21	20465.0
22	26063.0
23	33383.0
24	41576.0
25	50723.0
26	61044.0
27	72488.0
28	85531.0
29	100609.0
30	115586.0
31	133319.0
32	151918.0
33	169204.0
34	186142.0
35	198207.0
36	201073.0
37	175707.0
38	58253.0
39	88.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.24230385265774	23.905451623806247	24.235609279310765	22.616635244225243
2	26.43336746699994	30.520532360636288	21.713683067608017	21.332417104755756
3	19.140944710661405	26.206993908524588	29.727406864574313	24.9246545162397
4	22.620033714069812	27.099603901199508	28.317846451511112	21.96251593321957
5	22.159204439708883	27.280200831121977	27.466624593300885	23.093970135868254
6	21.7395952756731	28.846534111344603	26.476274306580933	22.93759630640136
7	22.331511480475587	27.882185579872875	27.843260796567144	21.94304214308439
8	22.230047545325316	27.46823348434419	27.501553098853897	22.800165871476594
9	21.783398631819818	26.45333463428609	28.620614669038137	23.142652064855955
10-11	22.586858058961184	26.67767113540479	27.388333879145293	23.34713692648873
12-13	22.772043766074507	27.026593503097978	27.013438827247647	23.187923903579865
14-15	22.789350695521183	27.094303096986312	26.91457435089663	23.201771856595872
16-17	22.88338860232973	27.0103075661911	26.861277197782734	23.245026633696433
18-19	23.027961697386704	27.164795635145865	26.768163102238407	23.039079565229024
20-21	22.911528592603663	27.028501260125637	26.850459208881915	23.20951093838878
22-23	22.85201368283983	26.84413945867563	26.975731176104546	23.328115682379995
24-25	22.824247337415073	26.886982670167477	27.14718187163852	23.14158812077893
26-27	23.02541061753909	26.998048051866498	26.848369285128186	23.128172045466222
28-29	23.049180685211127	26.940153924163102	26.911427434083475	23.099237956542297
30-31	22.99917531359103	26.926893547983617	26.925933403328745	23.14799773509661
32-33	23.01804085856654	26.937766537453683	26.805863421758332	23.23832918222144
34-35	23.147219239430495	26.813907876974845	26.852780760569505	23.18609212302515
36-37	23.047338245467987	26.92211877456478	26.94409830220475	23.08644467776248
38-39	23.077803375868605	26.806875466124282	26.977807164547514	23.1375139934596
40-41	23.128965540507227	26.66602655760592	26.917018510397018	23.287989391489834
42-43	22.802890606307994	27.001706981497236	27.13693167870134	23.058470733493426
44-45	23.009036258695147	26.682549708245773	27.124008650643844	23.184405382415235
46-47	23.09353460973929	26.725957726244538	26.981385998813835	23.199121665202345
48-49	22.99341028000471	26.802753181588646	27.154400403988188	23.049436134418457
50-51	23.167893360542642	26.765687932503333	26.904364068452658	23.162054638501363
52-53	23.051751658264514	26.822585872813754	26.986822892081825	23.138839576839906
54-55	22.86154107254397	26.79517458234841	27.307460650819475	23.035823694288144
56-57	23.152998901790145	26.904740156074514	26.848869508172218	23.093391433963127
58-59	23.098237861891302	26.77617713537362	27.00986653963577	23.11571846309931
60-61	23.198386103550224	26.826435287532398	26.91697562573131	23.05820298318607
62-63	23.30735461610743	26.8474511351773	26.745908111997828	23.099286136717442
64-65	23.31716190844439	26.80307927458249	26.77113433095325	23.10862448601987
66-67	23.307526679692398	26.948078825992326	26.754146144741302	22.990248349573974
68-69	23.452469063395	26.966852858956486	26.55443399585401	23.026244081794506
70-71	23.554588683969317	26.920156452578947	26.351650838368666	23.17360402508307
72-73	23.381981383755424	27.138431873303414	26.52932965788361	22.95025708505755
74-75	23.477878458729926	26.994396665413632	26.392110887042342	23.1356139888141
76-77	23.511376984085736	27.19016039000863	26.152588796607173	23.145873829298463
78-79	23.63538367240385	27.2933904993131	26.038529427487315	23.03269640079573
80-81	23.53639643309137	27.225239556858778	25.958513178484665	23.279850831565188
82-83	23.665277851666254	27.28745096868873	25.72841422066977	23.318856958975246
84-85	23.530269682930065	27.28393821834718	25.805520715984965	23.380271382737792
86-87	23.618319192258923	27.37786227647163	25.521849504089207	23.48196902718024
88-89	23.702447064355823	27.39085944529263	25.376046729682606	23.53064676066894
90-91	23.716027689169092	27.47685431520698	25.16623104934891	23.640886946275018
92-93	23.706880890613533	27.43952761937981	25.08701939496995	23.766572095036704
94-95	23.78935298671725	27.36429362454559	24.90626240091273	23.940090987824426
96-97	23.745599105573195	27.48358893946165	24.77339578541854	23.997416169546614
98-99	23.757276874313384	27.38414551698346	24.693372740246826	24.16520486845633
100-101	23.790126885175276	27.25583882027152	24.48743735547314	24.466596939080063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	2.5
5	7.5
6	11.5
7	20.5
8	33.0
9	46.5
10	70.5
11	110.5
12	154.5
13	220.0
14	356.5
15	560.5
16	854.0
17	1242.0
18	1853.5
19	2650.5
20	3723.5
21	5129.5
22	6761.5
23	8816.5
24	11286.0
25	14087.0
26	17304.5
27	20727.5
28	24303.5
29	28320.0
30	32306.0
31	36092.5
32	40218.0
33	44322.5
34	47956.5
35	51429.5
36	54794.5
37	57742.5
38	59830.0
39	60831.0
40	60978.5
41	60516.0
42	59227.5
43	57130.5
44	55215.5
45	53459.5
46	51511.0
47	49675.0
48	47666.0
49	45128.0
50	42896.5
51	40943.5
52	39002.5
53	37583.5
54	36452.5
55	35656.5
56	34992.0
57	34563.0
58	34064.5
59	33145.0
60	32328.5
61	31547.0
62	30302.0
63	28885.0
64	27670.0
65	26494.0
66	25287.5
67	23726.5
68	22040.5
69	20476.0
70	18836.0
71	17131.0
72	15260.5
73	13216.0
74	11286.5
75	9368.5
76	7460.5
77	5865.5
78	4490.0
79	3335.0
80	2424.0
81	1718.5
82	1190.0
83	799.0
84	519.0
85	348.5
86	248.0
87	168.5
88	122.5
89	93.0
90	62.0
91	43.0
92	30.0
93	19.0
94	8.0
95	3.5
96	3.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6516527722490169
2	0.002802584398012656
3	1.0379942214861689E-4
4	5.189971107430844E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.18281673225925152
14-15	2.594985553715422E-5
16-17	2.33548699834388E-4
18-19	0.10190508269440463
20-21	5.189971107430844E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.001972189020823721
42-43	0.0
44-45	0.0
46-47	0.021823828506746704
48-49	2.0759884429723377E-4
50-51	7.784956661146267E-5
52-53	2.33548699834388E-4
54-55	0.0019462391652865668
56-57	0.0010898939325604774
58-59	0.09352327935590384
60-61	0.0014272420545434824
62-63	0.0012196432102462487
64-65	0.002101938298509492
66-67	0.0048526229854478405
68-69	0.005760867929248238
70-71	0.0038924783305731335
72-73	0.03526585367499259
74-75	0.041364069726223834
76-77	0.14184191036608498
78-79	3.113982664458507E-4
80-81	0.1032285253267995
82-83	0.004696923852224915
84-85	0.005423519807265233
86-87	0.0070583607061059485
88-89	0.0071362102727174125
90-91	0.021175082118317846
92-93	0.010950839036679082
94-95	0.014090771556674745
96-97	0.010172343370564456
98-99	0.011807184269405172
100-101	0.012871128346428494
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1926793.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.5889586974637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.9756781178038	97.5790904200962
2	0.7686034368448129	1.5155162497964383
3	0.17398490244535203	0.5145897108350119
4	0.03827115814808156	0.1509245451986126
5	0.03068049439815179	0.1512378997518577
6	0.004270020822338509	0.025258614389450477
7	0.0073352126392730345	0.05062196831512821
8	0.0	0.0
9	0.0	0.0
>10	0.001176656898213361	0.012760591617294708
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.037994221486169E-4	0.0	0.0
2	1.5569913322292534E-4	0.0	0.0
3	1.5569913322292534E-4	0.0	0.0
4	1.5569913322292534E-4	0.0	5.189971107430845E-5
5	2.075988442972338E-4	0.0	5.189971107430845E-5
6	2.5949855537154225E-4	0.0	5.189971107430845E-5
7	3.6329797752015917E-4	0.0	5.189971107430845E-5
8	4.151976885944676E-4	5.189971107430845E-5	5.189971107430845E-5
9	6.227965328917014E-4	5.189971107430845E-5	5.189971107430845E-5
10-11	6.746962439660098E-4	5.189971107430845E-5	5.189971107430845E-5
12-13	7.265959550403183E-4	5.189971107430845E-5	5.189971107430845E-5
14-15	8.303953771889352E-4	5.189971107430845E-5	5.189971107430845E-5
16-17	0.0010120443659490147	5.189971107430845E-5	5.189971107430845E-5
18-19	0.0011158437880976317	5.189971107430845E-5	5.189971107430845E-5
20-21	0.001271542921320557	7.784956661146267E-5	5.189971107430845E-5
22-23	0.0015310414766920993	1.037994221486169E-4	5.189971107430845E-5
24-25	0.0016867406099150247	1.037994221486169E-4	5.189971107430845E-5
26-27	0.0021538380095838007	1.037994221486169E-4	5.189971107430845E-5
28-29	0.0033475313642928953	1.037994221486169E-4	5.189971107430845E-5
30-31	0.005371620096190924	1.037994221486169E-4	5.189971107430845E-5
32-33	0.008615352038335203	1.037994221486169E-4	5.189971107430845E-5
34-35	0.014324320256509131	1.037994221486169E-4	2.075988442972338E-4
36-37	0.022680173739472793	1.037994221486169E-4	2.5949855537154225E-4
38-39	0.03526585367499259	1.037994221486169E-4	3.373481219830049E-4
40-41	0.0556105404161215	1.037994221486169E-4	3.8924783305731335E-4
42-43	0.0853231250061631	1.2974927768577113E-4	4.151976885944676E-4
44-45	0.12712834227651854	1.5569913322292534E-4	4.151976885944676E-4
46-47	0.18798075351114518	1.5569913322292534E-4	4.151976885944676E-4
48-49	0.2643252285014529	1.5569913322292534E-4	4.151976885944676E-4
50-51	0.3633239273756963	1.5569913322292534E-4	4.151976885944676E-4
52-53	0.4911269658961809	1.5569913322292534E-4	4.151976885944676E-4
54-55	0.6506147780275306	1.8164898876007958E-4	4.151976885944676E-4
56-57	0.8506103146523782	2.075988442972338E-4	4.151976885944676E-4
58-59	1.0986130840209611	2.075988442972338E-4	4.930472552059303E-4
60-61	1.3923135489904728	2.075988442972338E-4	5.189971107430845E-4
62-63	1.7243419505883613	2.075988442972338E-4	5.189971107430845E-4
64-65	2.1064016736618827	2.075988442972338E-4	5.189971107430845E-4
66-67	2.5527910886120098	2.075988442972338E-4	5.70896821817393E-4
68-69	3.060785460607341	2.33548699834388E-4	5.70896821817393E-4
70-71	3.631500633435974	2.5949855537154225E-4	5.70896821817393E-4
72-73	4.2558022579488295	2.5949855537154225E-4	5.70896821817393E-4
74-75	4.921597701465596	3.113982664458507E-4	5.70896821817393E-4
76-77	5.663322422284075	3.113982664458507E-4	5.70896821817393E-4
78-79	6.479367529360964	3.113982664458507E-4	5.70896821817393E-4
80-81	7.342874922215309	3.113982664458507E-4	5.70896821817393E-4
82-83	8.275045632820962	3.113982664458507E-4	6.227965328917014E-4
84-85	9.26062114612208	3.113982664458507E-4	6.227965328917014E-4
86-87	10.29724002526478	3.373481219830049E-4	6.227965328917014E-4
88-89	11.38928779583484	3.8924783305731335E-4	6.746962439660098E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGTA	1365	0.0	9.736911	94-95
CGGACTA	220	8.9868536E-4	9.725786	18-19
CTCGTAT	1245	0.0	9.340982	94-95
CTACATC	2975	0.0	8.615939	94-95
TAGATCC	505	0.0032078703	8.4567995	3
GCTACAT	3140	0.0	8.389946	94-95
ATCTCGT	1950	0.0	8.276375	94-95
ACGGCTA	4015	0.0	8.275768	94-95
AGTCACG	5940	0.0	8.2308855	94-95
CGGCTAC	4085	0.0	8.192056	94-95
GTATGCC	1205	0.0	8.075374	94-95
GGCTACA	3595	0.0	7.922243	94-95
TACATCT	2860	0.0	7.883579	94-95
CGTATGC	1115	0.0	7.875764	94-95
CAGTCAC	7150	0.0	7.866982	94-95
TATGCCG	915	0.0	7.7815475	94-95
TCGTATG	1215	0.0	7.6182313	92-93
TCACGGC	5410	0.0	7.501786	94-95
GTCACGG	5785	0.0	7.4667883	94-95
CACGGCT	4815	0.0	7.4429736	94-95
>>END_MODULE