FastQCFastQC Report
Thu 11 Sep 2014
NYCLib12_GGCTAC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib12_GGCTAC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926793
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA13650.09.73691194-95
CGGACTA2208.9868536E-49.72578618-19
CTCGTAT12450.09.34098294-95
CTACATC29750.08.61593994-95
TAGATCC5050.00320787038.45679953
GCTACAT31400.08.38994694-95
ATCTCGT19500.08.27637594-95
ACGGCTA40150.08.27576894-95
AGTCACG59400.08.230885594-95
CGGCTAC40850.08.19205694-95
GTATGCC12050.08.07537494-95
GGCTACA35950.07.92224394-95
TACATCT28600.07.88357994-95
CGTATGC11150.07.87576494-95
CAGTCAC71500.07.86698294-95
TATGCCG9150.07.781547594-95
TCGTATG12150.07.618231392-93
TCACGGC54100.07.50178694-95
GTCACGG57850.07.466788394-95
CACGGCT48150.07.442973694-95
Produced by FastQC (version 0.11.2)