FastQCFastQC Report
Thu 11 Sep 2014
NYCLib11_TAGCTT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib11_TAGCTT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225116
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA4708.942152E-99.08899290-91
TCGTATG4302.0449625E-78.82902692-93
CGTATGC3304.9826666E-47.90917594-95
TATCTCG6504.5256456E-97.66581594-95
GTATGCC5356.888313E-77.539586594-95
CTCGTAT4401.9039123E-47.01040594-95
ATCTCGT7354.8084985E-86.77929394-95
TTCCACC8509.36653E-46.72575438
GCTTATC11302.0008883E-116.299342694-95
TAGCTTA12751.2732926E-115.955264692-93
CTAGCTT13903.6379788E-125.80385594-95
CACTAGC13354.5474735E-115.687612592-93
CACGTCT33600.05.508175494-95
CGTCTGA28500.05.32828694-95
CCAGTCA19750.05.28613294-95
ACTAGCT13954.7602953E-95.10373590-91
Produced by FastQC (version 0.11.2)