##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename NYCLib10_GATCAG_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1329249 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25499887530478 33.0 33.0 33.0 27.0 33.0 2 31.66463168300296 33.0 33.0 33.0 27.0 33.0 3 31.80615370032251 33.0 33.0 33.0 27.0 33.0 4 35.63702586949473 37.0 37.0 37.0 33.0 37.0 5 35.1590777950557 37.0 37.0 37.0 33.0 37.0 6 34.94267439734767 37.0 33.0 37.0 33.0 37.0 7 34.729344915813364 37.0 33.0 37.0 27.0 37.0 8 34.775628193062396 37.0 33.0 37.0 27.0 37.0 9 34.67434468636049 37.0 33.0 37.0 27.0 37.0 10-11 34.53124358190226 37.0 33.0 37.0 27.0 37.0 12-13 34.526350593455405 37.0 33.0 37.0 27.0 37.0 14-15 35.43675752248074 37.0 33.0 40.0 27.0 40.0 16-17 35.34113059328989 37.0 33.0 40.0 27.0 40.0 18-19 35.54254545235693 37.0 33.0 40.0 27.0 40.0 20-21 35.21516096683165 37.0 33.0 40.0 27.0 40.0 22-23 35.24364509583983 37.0 33.0 40.0 27.0 40.0 24-25 35.06794927060318 37.0 33.0 40.0 27.0 40.0 26-27 34.67497662213776 37.0 33.0 40.0 27.0 40.0 28-29 34.29815670352207 37.0 33.0 40.0 27.0 40.0 30-31 34.1673644290874 37.0 33.0 40.0 24.5 40.0 32-33 34.16889010260681 37.0 33.0 38.5 24.5 40.0 34-35 34.00597254539969 37.0 33.0 37.0 24.5 40.0 36-37 33.6916552128307 37.0 33.0 37.0 22.0 40.0 38-39 33.58644505280802 37.0 33.0 37.0 22.0 40.0 40-41 33.27552550349859 37.0 33.0 37.0 22.0 40.0 42-43 32.8708029872507 37.0 33.0 37.0 22.0 40.0 44-45 32.75873105791315 37.0 33.0 37.0 22.0 40.0 46-47 32.60395606842661 37.0 33.0 37.0 22.0 40.0 48-49 32.60946256119057 37.0 33.0 37.0 22.0 40.0 50-51 32.91454460375746 37.0 33.0 37.0 22.0 40.0 52-53 32.764094988974975 37.0 33.0 37.0 22.0 40.0 54-55 32.39481203295996 37.0 33.0 37.0 22.0 40.0 56-57 32.440566440147784 37.0 33.0 37.0 22.0 40.0 58-59 32.13807420581095 35.0 27.0 37.0 22.0 40.0 60-61 32.04748320292135 33.0 27.0 37.0 22.0 40.0 62-63 32.03006396845136 33.0 27.0 37.0 22.0 40.0 64-65 31.68495330822141 33.0 27.0 37.0 18.5 40.0 66-67 31.329199043971446 33.0 27.0 37.0 15.0 40.0 68-69 31.163729669911355 33.0 27.0 37.0 15.0 40.0 70-71 31.003398911716317 33.0 27.0 37.0 15.0 37.0 72-73 30.778333856184958 33.0 27.0 37.0 15.0 37.0 74-75 30.431843845660218 33.0 27.0 37.0 15.0 37.0 76-77 29.33288044602629 33.0 27.0 35.0 15.0 37.0 78-79 30.265512706799104 33.0 27.0 37.0 15.0 37.0 80-81 29.990122618109925 33.0 27.0 37.0 15.0 37.0 82-83 29.977053960544637 33.0 27.0 37.0 15.0 37.0 84-85 29.844889106555655 33.0 27.0 37.0 15.0 37.0 86-87 29.642183669124446 33.0 27.0 37.0 15.0 37.0 88-89 29.507738580205817 33.0 27.0 37.0 15.0 37.0 90-91 29.419969471483522 33.0 27.0 35.0 15.0 37.0 92-93 29.21110303637618 33.0 27.0 33.0 15.0 37.0 94-95 29.11618515417352 33.0 27.0 33.0 15.0 37.0 96-97 28.96451153997483 33.0 27.0 33.0 15.0 37.0 98-99 28.695719161722145 33.0 27.0 33.0 15.0 37.0 100-101 27.884840989160043 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.037186129604958 2306 2 0.5189509923534779 2306 3 0.40631211143287516 2306 4 0.4260694526034001 2306 5 0.7301597199614704 2306 6 0.7942406624644747 2306 7 0.9015667299897387 2306 8 0.6985981555411058 2306 9 0.95344682136313 2306 10-11 1.2222954992709631 2306 12-13 1.423938392256055 2306 14-15 1.5531693448773467 2306 16-17 1.568668278043468 2306 18-19 1.400235225221806 2306 20-21 1.6563742759634792 2306 22-23 1.230780894116144 2306 24-25 1.1983239642836665 2306 26-27 1.3494965341632792 2306 28-29 1.722493850239573 2306 30-31 1.567901359855398 2306 32-33 1.3441096979327583 2306 34-35 1.1929233936063852 2306 36-37 1.249594156600189 2306 38-39 0.9177379936470302 2306 40-41 1.1034742523656078 2306 42-43 1.132872323098347 2306 44-45 0.9156110799442629 2306 46-47 1.1314957871936073 2306 48-49 1.1034038576273062 2306 50-51 1.1008870939028625 2306 52-53 1.2419655048892508 2306 54-55 1.4043646556300402 2306 56-57 0.9863004576282464 2306 58-59 0.9257357885007558 2306 60-61 0.7524362968101315 2306 62-63 0.6660041915228092 2306 64-65 0.751029735122966 2306 66-67 0.8818756632409865 2306 68-69 0.6283409642960329 2306 70-71 0.6682285274486546 2306 72-73 0.42682852890498424 2306 74-75 0.6619710407022907 2306 76-77 0.6825551193887982 2306 78-79 0.8117224808465089 2306 80-81 1.2131673252235302 2306 82-83 0.9065445147443967 2306 84-85 0.8095171314387741 2306 86-87 0.78737038671429 2306 88-89 0.433109893838747 2306 90-91 0.2703827189106498 2306 92-93 0.11891079764918544 2306 94-95 -0.06896647774318154 2306 96-97 0.02054878566329421 2306 98-99 0.19071957103541948 2306 100-101 0.01684608788572106 2307 1 0.5987164396172062 2307 2 0.3968898845234392 2307 3 0.28420964106127045 2307 4 0.36380334755914134 2307 5 0.6023079801526592 2307 6 0.6606566192908971 2307 7 0.7805056297139359 2307 8 0.7415635575088473 2307 9 0.9370808772984134 2307 10-11 1.1650759338971497 2307 12-13 1.323221160831764 2307 14-15 1.1838218488776846 2307 16-17 1.3644981908143237 2307 18-19 1.240260827891987 2307 20-21 1.476988091126664 2307 22-23 1.292215599691012 2307 24-25 1.2478589514829466 2307 26-27 1.3059592393304627 2307 28-29 1.5109159671125312 2307 30-31 1.5713872066215515 2307 32-33 1.210064784404615 2307 34-35 1.1601889048381082 2307 36-37 1.5225145464631993 2307 38-39 1.0518794404121437 2307 40-41 1.0603885398914912 2307 42-43 1.412167621799675 2307 44-45 1.2245594199874503 2307 46-47 1.2145400058558167 2307 48-49 1.000537198201151 2307 50-51 1.0390876177705977 2307 52-53 1.2414685351907764 2307 54-55 1.5755687353141141 2307 56-57 1.2349335432775632 2307 58-59 1.1692535413902796 2307 60-61 1.0185538489055688 2307 62-63 0.9490001373745329 2307 64-65 1.1211846056427284 2307 66-67 1.1206871267083862 2307 68-69 1.0841521475136382 2307 70-71 0.9870375611763436 2307 72-73 0.6582557879839683 2307 74-75 0.9192577520941647 2307 76-77 1.0958244951698397 2307 78-79 0.9623203986759812 2307 80-81 1.7277095403411948 2307 82-83 1.271018599316431 2307 84-85 1.087463627381343 2307 86-87 1.1271107240716383 2307 88-89 1.050262853136644 2307 90-91 0.9338157801387297 2307 92-93 0.7392974655856257 2307 94-95 0.6305274750903962 2307 96-97 0.6357360954374016 2307 98-99 0.8869432205267636 2307 100-101 0.8087100264244498 1101 1 1.0671253153087683 1101 2 0.5402876041172107 1101 3 0.3937509576741718 1101 4 0.36899714139445194 1101 5 0.5857393266453244 1101 6 0.5735548881905785 1101 7 0.8604243630232347 1101 8 0.6222779218488625 1101 9 0.9139786871899176 1101 10-11 1.0291196569539593 1101 12-13 1.2650995658743867 1101 14-15 1.3282812144581158 1101 16-17 1.3523268280971408 1101 18-19 0.9793247873708708 1101 20-21 1.407184914896206 1101 22-23 1.0169845253426786 1101 24-25 1.0882845425539287 1101 26-27 1.1637266068566205 1101 28-29 1.5409242588324545 1101 30-31 1.3966727945103088 1101 32-33 0.9205498607621152 1101 34-35 1.046270138923056 1101 36-37 1.038006957220425 1101 38-39 0.8219256938273745 1101 40-41 0.9506655536034785 1101 42-43 1.050526614473867 1101 44-45 0.8767023701993466 1101 46-47 0.7096503428540117 1101 48-49 0.7699540846723281 1101 50-51 0.7839612786755268 1101 52-53 1.1398539562214651 1101 54-55 1.4750561172019658 1101 56-57 1.1932120123690098 1101 58-59 1.073003810103426 1101 60-61 1.0123993226387924 1101 62-63 0.7265450198945942 1101 64-65 0.7389200144715353 1101 66-67 1.0064168378706704 1101 68-69 0.6498225440009122 1101 70-71 0.7302061179027106 1101 72-73 0.6340651595610787 1101 74-75 0.7417817081633409 1101 76-77 0.8781210349280748 1101 78-79 0.7666924263231358 1101 80-81 1.400764534247834 1101 82-83 1.1956658094452415 1101 84-85 0.8935324813888137 1101 86-87 0.5954090182218259 1101 88-89 0.6440531245644081 1101 90-91 0.5260289692181246 1101 92-93 0.7749920497314164 1101 94-95 0.7340738819928063 1101 96-97 0.6780224768592973 1101 98-99 0.6232303827175336 1101 100-101 0.6575865116451354 2201 1 1.4216796154638018 2201 2 0.8235448521898334 2201 3 0.2641222492242967 2201 4 0.3061983494128384 2201 5 0.4425505590758121 2201 6 0.35923977989626366 2201 7 0.43177530412140896 2201 8 0.5565532954730941 2201 9 0.5456223926137156 2201 10-11 0.8138905080461711 2201 12-13 0.9505116962541535 2201 14-15 1.167430416184672 2201 16-17 0.9978175610706543 2201 18-19 0.88688044191138 2201 20-21 1.2548116501790503 2201 22-23 0.8679731061471898 2201 24-25 0.8505829203814983 2201 26-27 0.9376846080941803 2201 28-29 1.3637965282986286 2201 30-31 1.4445488512814393 2201 32-33 1.1387123809893396 2201 34-35 1.0366304114884244 2201 36-37 1.0683798239879323 2201 38-39 0.6411966319710771 2201 40-41 0.9428022794576236 2201 42-43 0.886304737945288 2201 44-45 0.7976705485471101 2201 46-47 0.8873516334017282 2201 48-49 0.9285818668340866 2201 50-51 0.8804800859420254 2201 52-53 1.050326589268927 2201 54-55 1.3307609290011797 2201 56-57 1.0781947641155014 2201 58-59 1.165361857768449 2201 60-61 0.9176189071883982 2201 62-63 0.6499037225184239 2201 64-65 0.8470835597205912 2201 66-67 0.9160451468634996 2201 68-69 0.7226925523895069 2201 70-71 0.9256448084071671 2201 72-73 0.7271090674266851 2201 74-75 0.8458040898166566 2201 76-77 0.9629063569168288 2201 78-79 0.8872900140548303 2201 80-81 1.2766860175750239 2201 82-83 1.0187204725032295 2201 84-85 0.8933534794133813 2201 86-87 1.0810449575978147 2201 88-89 0.8608655930506401 2201 90-91 0.7687516910128771 2201 92-93 0.8616284405692305 2201 94-95 0.8447560792477056 2201 96-97 0.4332849465137514 2201 98-99 0.602309008675757 2201 100-101 0.8567401526206808 2304 1 -1.8590469498235649 2304 2 -0.2634302350299329 2304 3 -0.12831095786513558 2304 4 -0.022361238872711908 2304 5 0.29453312992307445 2304 6 0.4351613619348029 2304 7 0.4251576549786762 2304 8 0.6260326283485611 2304 9 0.838059661928888 2304 10-11 0.768683682499244 2304 12-13 1.1016332641851108 2304 14-15 0.9918248060304933 2304 16-17 1.0441019785931402 2304 18-19 0.8288401320359782 2304 20-21 1.0769759095833393 2304 22-23 0.8647330874924393 2304 24-25 1.0134969879322213 2304 26-27 1.0274244493020959 2304 28-29 1.4947652841144716 2304 30-31 1.4353135857457175 2304 32-33 0.8941915047703972 2304 34-35 0.9348779547078294 2304 36-37 0.993948748149954 2304 38-39 0.8259592547077617 2304 40-41 0.9917883132736023 2304 42-43 1.0023108048321987 2304 44-45 0.8575249256268407 2304 46-47 0.8456839826687457 2304 48-49 0.9217351270536511 2304 50-51 0.7989500344462286 2304 52-53 0.7518325098842169 2304 54-55 1.1644464793493157 2304 56-57 0.9971498966747419 2304 58-59 0.7121044565663475 2304 60-61 0.349829098084669 2304 62-63 0.3827864794408242 2304 64-65 0.5484432087191031 2304 66-67 0.4982645171303375 2304 68-69 0.6659249945208998 2304 70-71 0.5053849613672838 2304 72-73 0.2946239677651725 2304 74-75 0.5121777408979185 2304 76-77 0.518133811642695 2304 78-79 0.5977466869533927 2304 80-81 1.3390964478592018 2304 82-83 0.9085551934487874 2304 84-85 0.5431965483902914 2304 86-87 0.3650292547335674 2304 88-89 0.2646286311420383 2304 90-91 0.16221076755245178 2304 92-93 0.3347063543738358 2304 94-95 0.11375044849898686 2304 96-97 -0.01212629716027891 2304 98-99 0.4957097497642913 2304 100-101 0.35707827780588275 1102 1 1.4259957940935237 1102 2 0.14564167690550178 1102 3 0.2656216727167475 1102 4 0.19064392662439644 1102 5 0.3412697017857127 1102 6 0.22436262681062402 1102 7 -5.148197167958074 1102 8 -1.1664903228724697 1102 9 0.1409489444603409 1102 10-11 0.6683503446869778 1102 12-13 0.9138437727198507 1102 14-15 0.9679975742054054 1102 16-17 0.6786801837587078 1102 18-19 0.8864369691833076 1102 20-21 1.1659291342715505 1102 22-23 0.7309858710516366 1102 24-25 1.022345569466573 1102 26-27 1.1721594935554762 1102 28-29 0.8079754396273202 1102 30-31 1.1350799072490005 1102 32-33 0.957932196501325 1102 34-35 1.0892241635966116 1102 36-37 1.3886327063157253 1102 38-39 0.9351393417608946 1102 40-41 1.1831218093611326 1102 42-43 1.0933937653228867 1102 44-45 1.1511619112911475 1102 46-47 0.9225928442159415 1102 48-49 0.9838778843552163 1102 50-51 0.8043141172707777 1102 52-53 0.9618389089511084 1102 54-55 1.410633607470082 1102 56-57 1.0487725845166764 1102 58-59 0.9077079817594189 1102 60-61 0.85015681686356 1102 62-63 0.6363153458287556 1102 64-65 0.40540137815669297 1102 66-67 0.5971028779570915 1102 68-69 0.7930232541387383 1102 70-71 0.6690192404723518 1102 72-73 0.48182442049872165 1102 74-75 0.5013590948511251 1102 76-77 0.4864290265820621 1102 78-79 0.7678705133475994 1102 80-81 1.3078010495159802 1102 82-83 0.9896657657298888 1102 84-85 0.7469242011717192 1102 86-87 0.6074364486319261 1102 88-89 0.5124916970436999 1102 90-91 0.3642541063541671 1102 92-93 0.30218105083696756 1102 94-95 0.2387558799404097 1102 96-97 0.03746821628989139 1102 98-99 0.1548544735530264 1102 100-101 0.3472627688200589 2202 1 1.4863572462623296 2202 2 0.8714133150517966 2202 3 0.4000662301551827 2202 4 -0.2875148656408939 2202 5 0.13867965478333133 2202 6 0.465326931259888 2202 7 0.6603420688305732 2202 8 -3.8788806008695254 2202 9 -0.23649362197403434 2202 10-11 0.6588421813081524 2202 12-13 0.10422803406783032 2202 14-15 0.18881726632362472 2202 16-17 0.26145124713391965 2202 18-19 0.7069529446919489 2202 20-21 1.2243637267637126 2202 22-23 -2.5847262055275735 2202 24-25 -2.970198793628441 2202 26-27 -1.484860045852308 2202 28-29 0.7854930690308777 2202 30-31 1.2432748057763376 2202 32-33 1.180897775597721 2202 34-35 -2.1125067232982104 2202 36-37 -0.28642882078494125 2202 38-39 0.5081052111457112 2202 40-41 1.0732717985830078 2202 42-43 1.0395777553729246 2202 44-45 1.1378795301319542 2202 46-47 0.7289985546949964 2202 48-49 0.9925994597336611 2202 50-51 -3.7773178917667423 2202 52-53 -0.01107924076168132 2202 54-55 1.7496360865332647 2202 56-57 1.5899912939991978 2202 58-59 1.4456830457961125 2202 60-61 1.6470806772052384 2202 62-63 1.4426835060949443 2202 64-65 1.3391174801936394 2202 66-67 1.3944304264975322 2202 68-69 1.3577732118596337 2202 70-71 1.2675720683779446 2202 72-73 1.2026133982283547 2202 74-75 0.7693132000632623 2202 76-77 1.4020665271275234 2202 78-79 1.3114865370441215 2202 80-81 1.9715224123283832 2202 82-83 1.9082049332238746 2202 84-85 1.4989154393305277 2202 86-87 1.4569636624229823 2202 88-89 1.401370222947822 2202 90-91 1.2177323329428233 2202 92-93 1.2387906730181513 2202 94-95 1.2221621505824949 2202 96-97 1.4025282956940757 2202 98-99 1.4687648890456622 2202 100-101 1.4612707399530258 2305 1 1.1542551093464972 2305 2 0.7174038989973397 2305 3 0.20058908035474232 2305 4 -0.09175739245891634 2305 5 0.1989482570901373 2305 6 0.28745457440847844 2305 7 0.5720142774718084 2305 8 -0.05054755810279232 2305 9 0.5500608223587022 2305 10-11 0.36299172681533065 2305 12-13 0.9840140582811472 2305 14-15 0.6086040243766675 2305 16-17 0.5219287180422683 2305 18-19 0.6025262792469377 2305 20-21 0.6460073538323101 2305 22-23 0.47039682291443796 2305 24-25 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0.16219002261728122 1208 44-45 0.08413083837765356 1208 46-47 -0.22247983668491855 1208 48-49 -1.712770040767218 1208 50-51 -0.4408329694827344 1208 52-53 -1.5540593153095656 1208 54-55 -1.0117422587114504 1208 56-57 -6.605482736365079 1208 58-59 -2.28082664318654 1208 60-61 -1.3605581797272563 1208 62-63 0.2238899593597452 1208 64-65 -0.15462889890086373 1208 66-67 0.43897154641484093 1208 68-69 0.19785396616430617 1208 70-71 -4.1861186888883495 1208 72-73 -0.6850745201300334 1208 74-75 -5.918970936152398 1208 76-77 -1.0906326654474228 1208 78-79 -3.659648282763097 1208 80-81 0.7337255275755368 1208 82-83 0.4588492331664149 1208 84-85 -2.9525393534799313 1208 86-87 -0.958693870417374 1208 88-89 -4.7861280809050015 1208 90-91 -0.0857594293865418 1208 92-93 -1.846315797827586 1208 94-95 -4.763735934275601 1208 96-97 -3.4217892041790776 1208 98-99 -6.463003387161432 1208 100-101 -3.51430651055405 1209 1 1.6752141762568584 1209 2 1.316103001415783 1209 3 0.5531390081472267 1209 4 0.36459991996861874 1209 5 0.8444776384300852 1209 6 1.136723927330337 1209 7 1.0793309183258657 1209 8 0.743097308770956 1209 9 0.39134376249765523 1209 10-11 -2.7556726811266223 1209 12-13 0.029015176989148017 1209 14-15 -0.6939374767841713 1209 16-17 -0.13055118153553025 1209 18-19 -16.47551014123617 1209 20-21 -14.071542850005002 1209 22-23 -2.9992658503036864 1209 24-25 -2.1260572616770332 1209 26-27 -1.741217878078956 1209 28-29 -3.2572750565412463 1209 30-31 -0.4641371278503499 1209 32-33 -0.8327975657867839 1209 34-35 -3.12903034938844 1209 36-37 -3.566023195813017 1209 38-39 -0.6184705972620108 1209 40-41 -9.725975747577976 1209 42-43 -4.680645874279769 1209 44-45 -3.4806412428912274 1209 46-47 -3.253821861617361 1209 48-49 -2.1362834297634308 1209 50-51 -2.444573913488462 1209 52-53 -1.6180150436553333 1209 54-55 -2.202832157219653 1209 56-57 -0.9684230078899887 1209 58-59 -1.4844191348950346 1209 60-61 -1.3328533707888646 1209 62-63 -0.9339840744205645 1209 64-65 -0.086871606282795 1209 66-67 0.29413086994654947 1209 68-69 -2.0109276203623665 1209 70-71 -1.5402811458142835 1209 72-73 -0.04931071824245592 1209 74-75 0.6057931162989512 1209 76-77 -16.334662916307558 1209 78-79 -16.37923913637728 1209 80-81 -21.619065564208935 1209 82-83 -9.202222856454586 1209 84-85 -2.6412906568156593 1209 86-87 -1.510870407410554 1209 88-89 -0.8885978852766634 1209 90-91 -5.448927363690338 1209 92-93 -3.3598624067298744 1209 94-95 0.08160290607217036 1209 96-97 0.8716735062742771 1209 98-99 1.6972658207068072 1209 100-101 1.5603156904735336 1212 1 -28.647261393775715 1212 2 -16.281896262618673 1212 3 -5.911752709514584 1212 4 -1.3071321885726732 1212 5 -0.7111831632271972 1212 6 -2.3041943941230585 1212 7 -0.44732738922596127 1212 8 -1.4929704545752287 1212 9 -0.30678770275522993 1212 10-11 0.613918312835736 1212 12-13 0.298729513325398 1212 14-15 -0.03153441298686488 1212 16-17 0.10352334179048484 1212 18-19 -0.8532206069388124 1212 20-21 -0.5944804428525146 1212 22-23 -0.24632539536794695 1212 24-25 0.6482636738169489 1212 26-27 -0.30882789811357014 1212 28-29 0.2928898542570053 1212 30-31 -0.34791590205884404 1212 32-33 0.5771522795241637 1212 34-35 -1.4361119734190737 1212 36-37 -1.1289107702613776 1212 38-39 -0.2468727342878836 1212 40-41 0.2774894235340071 1212 42-43 0.5800290333838163 1212 44-45 1.311649518015372 1212 46-47 -2.2434297555292915 1212 48-49 -1.5186039018716997 1212 50-51 0.2966285724396016 1212 52-53 -0.4473085167312618 1212 54-55 -0.28494342430700215 1212 56-57 -0.9059817829162249 1212 58-59 0.5317107766528117 1212 60-61 0.7130797381204061 1212 62-63 -4.469187759699889 1212 64-65 -3.6072333197196222 1212 66-67 -1.1588218510816226 1212 68-69 0.6224354696026069 1212 70-71 -0.655640337887661 1212 72-73 -0.7046539643956713 1212 74-75 0.632901180573274 1212 76-77 1.5297865405771311 1212 78-79 -0.6592978773310385 1212 80-81 0.610599980337426 1212 82-83 0.9230377980095881 1212 84-85 -2.816610597393261 1212 86-87 -1.6023618279603475 1212 88-89 -0.41574002202996496 1212 90-91 0.20770382216822014 1212 92-93 -2.363838665026524 1212 94-95 -1.725652487601252 1212 96-97 0.5006855406738531 1212 98-99 -0.19417926127157514 1212 100-101 -0.06946638195093158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 2.0 10 5.0 11 10.0 12 49.0 13 142.0 14 415.0 15 1152.0 16 2028.0 17 3407.0 18 5101.0 19 7252.0 20 10087.0 21 13307.0 22 17276.0 23 22055.0 24 27199.0 25 33391.0 26 40538.0 27 48731.0 28 57133.0 29 67098.0 30 78352.0 31 90619.0 32 103473.0 33 116942.0 34 128864.0 35 139562.0 36 143777.0 37 126473.0 38 44705.0 39 102.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.24763876270787 26.214071875570653 23.202371958995357 24.335917402726114 2 26.879418964892032 30.217858072515035 22.29806487142025 20.604658091172677 3 18.910466669876502 25.915404800308146 30.268091432148104 24.90603709766725 4 22.22895953200607 26.743767904860494 28.77183189291991 22.255440670213535 5 22.0115268094992 27.114032058703824 27.767784666379285 23.10665646541769 6 21.733174145701824 28.482699629640496 26.782040084288194 23.00208614036949 7 22.415439093804096 28.254074293078276 27.707374615290288 21.623111997827344 8 21.689314793541314 27.40344359860342 27.997237537887937 22.91000406996733 9 21.62649736806272 26.385726075400473 28.964701120708007 23.0230754358288 10-11 22.371203589395215 26.811906572809157 27.6289092562793 23.187980581516328 12-13 22.652320995694488 27.020883835642874 27.19398615482953 23.13280901383311 14-15 22.77808747646227 27.11647704831826 26.78027216872084 23.32516330649863 16-17 22.9308656677792 27.016980685659647 26.83003252597056 23.222121120590593 18-19 23.036086302238598 27.104456860178384 26.671981598335776 23.18747523924724 20-21 23.038468728759145 26.961286772187442 26.767530676167773 23.232713822885636 22-23 22.789748196161895 26.936901964944116 26.976247490124123 23.29710234876987 24-25 22.71598474025559 26.951383826506547 27.192535032939652 23.140096400298212 26-27 22.948333984076726 26.99919277727499 26.890823314518197 23.161649924130092 28-29 23.010022952810196 26.933215672910045 26.92640731721446 23.130354057065304 30-31 23.00577243240356 26.951985670103944 26.821874607391088 23.220367290101404 32-33 23.084162560964874 26.871451473726893 26.78109970366726 23.26328626164097 34-35 23.16270315042554 26.839591378289544 26.794942106407454 23.20276336487746 36-37 23.037670143065746 26.94325893794165 26.9153108258874 23.103760093105205 38-39 23.15401403348808 26.820858996320478 26.923623790576485 23.101503179614955 40-41 23.097470064289414 26.765777162380893 26.852293839222675 23.28445893410702 42-43 22.830410254211213 26.960637171816565 27.11696604624115 23.091986527731073 44-45 22.998926461483137 26.70752432388514 27.028081269950178 23.265467944681546 46-47 23.028411581345388 26.810314177196616 26.965662676667062 23.195611564790937 48-49 23.047476109603366 26.815660740381308 27.064109580796654 23.072753569218673 50-51 23.131491214772858 26.769411438117103 26.818461709755546 23.28063563735449 52-53 23.047927492810782 26.85662376645433 26.945132490375194 23.150316250359694 54-55 22.853756347220763 26.738881760667038 27.28224410717987 23.125117784932332 56-57 23.093925395181646 26.81852395051122 26.878445837648172 23.20910481665896 58-59 22.994001018155092 26.771190729366545 27.10310778286186 23.131700469616508 60-61 23.133462631636757 26.771406818705024 26.92600806785302 23.1691224818052 62-63 23.191098318427482 26.755675457707913 26.854190991489823 23.199035232374783 64-65 23.098537827245366 26.71708719508915 26.951058910090538 23.233316067574943 66-67 23.156647444505886 26.877508567215497 26.909069022980077 23.056774965298548 68-69 23.222269954204883 26.78382402396664 26.78773620748416 23.20616981434432 70-71 23.31211010499517 26.766621225936728 26.693193821264455 23.228074847803647 72-73 23.07681308302195 26.884390910219796 26.9330458440107 23.105750162747555 74-75 23.178279608495274 26.640542768945004 26.925866007962107 23.25531161459762 76-77 23.094204277234738 26.847945868661427 26.85838182410706 23.19946802999678 78-79 23.233781870993734 26.90563932697158 26.75634410648748 23.10423469554721 80-81 23.192864892631064 26.802785442519085 26.714549384136415 23.289800280713436 82-83 23.16306885336676 26.83083719597017 26.734086324949008 23.272007625714064 84-85 23.126421215968403 26.77308506136267 26.830789486058208 23.26970423661071 86-87 23.20671581799188 26.886707812939072 26.61153412247806 23.295042246590988 88-89 23.16778146807703 26.859134017298892 26.614581158558355 23.358503356065725 90-91 23.238306577076568 26.865403781882048 26.55730143125187 23.33898820978952 92-93 23.239302567744886 26.864489159614298 26.46790619846152 23.428302074179296 94-95 23.318306359897207 26.825837465656978 26.419420723529395 23.436435450916424 96-97 23.359711867556584 26.924463043342506 26.363825143272457 23.351999945828453 98-99 23.30024441434043 26.86146330342703 26.345247576265212 23.49304470596733 100-101 23.412216368119992 26.78914510252412 26.154306276345814 23.644332253010074 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 3.0 3 5.5 4 9.5 5 10.5 6 11.5 7 22.0 8 39.5 9 55.5 10 84.0 11 128.0 12 170.5 13 260.0 14 403.5 15 554.5 16 778.0 17 1123.5 18 1620.0 19 2264.0 20 3074.5 21 4221.0 22 5545.5 23 7123.0 24 9112.0 25 11267.5 26 13657.5 27 16376.5 28 19176.0 29 21971.5 30 24484.5 31 27301.0 32 30105.0 33 32477.5 34 34953.5 35 37086.0 36 38811.5 37 40319.5 38 41052.0 39 41267.0 40 41068.0 41 39877.5 42 38428.5 43 36758.0 44 35286.0 45 34027.5 46 32633.0 47 31452.5 48 30025.5 49 28533.5 50 27151.5 51 25623.0 52 24298.5 53 23282.5 54 22778.5 55 22513.0 56 22113.0 57 21869.5 58 21903.5 59 21746.5 60 21377.0 61 21256.5 62 21046.5 63 20532.5 64 20075.5 65 19668.5 66 19094.5 67 18189.5 68 17091.5 69 16192.5 70 15076.5 71 13643.0 72 12082.5 73 10458.5 74 8803.5 75 7060.0 76 5565.5 77 4277.5 78 3041.5 79 2092.5 80 1423.0 81 961.5 82 647.5 83 410.5 84 287.0 85 191.5 86 114.0 87 90.5 88 66.0 89 44.0 90 32.0 91 22.0 92 12.5 93 9.5 94 9.0 95 5.5 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7143883501134851 2 0.0026330657386238396 3 7.523044967496684E-5 4 7.523044967496684E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.19386886881238957 14-15 0.0 16-17 2.63306573862384E-4 18-19 0.11608058384847385 20-21 3.761522483748342E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0019183764667116544 42-43 0.0 44-45 0.0 46-47 0.022681980577002506 48-49 1.1284567451245026E-4 50-51 3.761522483748342E-5 52-53 1.1284567451245026E-4 54-55 0.0019183764667116544 56-57 0.0013917633189868867 58-59 0.10208772020893 60-61 0.0017303003425242372 62-63 0.0012789176444744363 64-65 0.002144067815736555 66-67 0.00406244428244821 68-69 0.005115670577897745 70-71 0.0033853702353735075 72-73 0.037916146636183284 74-75 0.043671276036318256 76-77 0.15847294224031763 78-79 2.63306573862384E-4 80-81 0.11739711671778574 82-83 0.004664287879847944 84-85 0.004626672655010461 86-87 0.006657894796234565 88-89 0.006657894796234565 90-91 0.023133363275052304 92-93 0.010908415202870192 94-95 0.01463232246178105 96-97 0.010381802055145424 98-99 0.011547874025107409 100-101 0.012902022119256812 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1329249.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.25963340285648 #Duplication Level Percentage of deduplicated Percentage of total 1 98.86625731371163 97.14562199557784 2 0.7653580405132152 1.5040760096551422 3 0.20044652099924237 0.5908740501079058 4 0.11549724634879871 0.45394868341089467 5 0.03604068466516395 0.17706722313934808 6 0.0107586632938199 0.06342853866933071 7 0.005549655404023985 0.038171497385810466 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 9.187506411803481E-5 0.026812002053733047 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.266131477247679E-4 0.0 0.0 2 5.266131477247679E-4 0.0 7.523044967496684E-5 3 7.523044967496684E-4 0.0 7.523044967496684E-5 4 9.027653960996022E-4 0.0 7.523044967496684E-5 5 9.77995845774569E-4 0.0 7.523044967496684E-5 6 0.0012036871947994695 0.0 7.523044967496684E-5 7 0.00142937854382437 0.0 7.523044967496684E-5 8 0.0015798394431743037 0.0 7.523044967496684E-5 9 0.0015798394431743037 0.0 7.523044967496684E-5 10-11 0.0017303003425242374 0.0 7.523044967496684E-5 12-13 0.002068837366061588 0.0 7.523044967496684E-5 14-15 0.002407374389598939 0.0 1.5046089934993368E-4 16-17 0.00285875708764874 0.0 1.5046089934993368E-4 18-19 0.003385370235373508 0.0 1.5046089934993368E-4 20-21 0.003648676809235892 0.0 1.5046089934993368E-4 22-23 0.003761522483748342 0.0 1.5046089934993368E-4 24-25 0.003987213832773243 0.0 1.5046089934993368E-4 26-27 0.004325750856310593 0.0 1.5046089934993368E-4 28-29 0.005115670577897745 0.0 1.5046089934993368E-4 30-31 0.006394588222372181 3.761522483748342E-5 1.5046089934993368E-4 32-33 0.008124888564896418 7.523044967496684E-5 1.5046089934993368E-4 34-35 0.011397413125757476 7.523044967496684E-5 2.2569134902490054E-4 36-37 0.015986470555930454 7.523044967496684E-5 2.2569134902490054E-4 38-39 0.022381058778302633 7.523044967496684E-5 2.2569134902490054E-4 40-41 0.03249955425958567 7.523044967496684E-5 3.0092179869986737E-4 42-43 0.04540157637884249 7.523044967496684E-5 3.0092179869986737E-4 44-45 0.06059812721318579 7.523044967496684E-5 3.0092179869986737E-4 46-47 0.08369387526340061 7.523044967496684E-5 3.0092179869986737E-4 48-49 0.11115298939476351 7.523044967496684E-5 3.0092179869986737E-4 50-51 0.14463053950012375 7.523044967496684E-5 3.0092179869986737E-4 52-53 0.18190722731406983 7.523044967496684E-5 3.0092179869986737E-4 54-55 0.2273840341425873 7.523044967496684E-5 3.3853702353735075E-4 56-57 0.28655278281194874 7.523044967496684E-5 3.761522483748342E-4 58-59 0.35113812385790777 7.523044967496684E-5 3.761522483748342E-4 60-61 0.4248263493145378 7.523044967496684E-5 3.761522483748342E-4 62-63 0.5130340515584364 7.523044967496684E-5 3.761522483748342E-4 64-65 0.6116235558574805 7.523044967496684E-5 3.761522483748342E-4 66-67 0.7228517757019188 7.523044967496684E-5 3.761522483748342E-4 68-69 0.8487875484578133 7.523044967496684E-5 3.761522483748342E-4 70-71 0.9885657239539019 7.523044967496684E-5 3.761522483748342E-4 72-73 1.1428257610124213 7.523044967496684E-5 3.761522483748342E-4 74-75 1.3091226700189353 7.523044967496684E-5 3.761522483748342E-4 76-77 1.5005465492168886 7.523044967496684E-5 3.761522483748342E-4 78-79 1.7145019480924941 7.523044967496684E-5 3.761522483748342E-4 80-81 1.9529824735621393 7.523044967496684E-5 3.761522483748342E-4 82-83 2.2084650806583266 7.523044967496684E-5 3.761522483748342E-4 84-85 2.4848241375393174 7.523044967496684E-5 3.761522483748342E-4 86-87 2.793494672555706 7.523044967496684E-5 3.761522483748342E-4 88-89 3.132257387442082 7.523044967496684E-5 3.761522483748342E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 495 1.72217E-6 7.6724553 46-47 CGTATGC 570 1.40941775E-5 6.662922 44-45 AGTCACG 1430 0.0 6.305389 94-95 CTCGTAT 525 0.0016135586 5.8776484 42-43 CCGTCTT 675 1.6617298E-4 5.6237154 50-51 GTCACGA 1610 1.8189894E-12 5.4530587 94-95 CGTCTGA 2835 0.0 5.440322 94-95 CGGAAGA 5510 0.0 5.4260435 94-95 TCGTATG 660 7.383167E-4 5.394695 42-43 CCAGTCA 2010 0.0 5.312278 94-95 TCGGAAG 5800 0.0 5.2365627 94-95 GTATGCC 645 0.0032295901 5.1521425 44-45 >>END_MODULE