FastQCFastQC Report
Thu 11 Sep 2014
NYCLib10_GATCAG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNYCLib10_GATCAG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329249
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4951.72217E-67.672455346-47
CGTATGC5701.40941775E-56.66292244-45
AGTCACG14300.06.30538994-95
CTCGTAT5250.00161355865.877648442-43
CCGTCTT6751.6617298E-45.623715450-51
GTCACGA16101.8189894E-125.453058794-95
CGTCTGA28350.05.44032294-95
CGGAAGA55100.05.426043594-95
TCGTATG6607.383167E-45.39469542-43
CCAGTCA20100.05.31227894-95
TCGGAAG58000.05.236562794-95
GTATGCC6450.00322959015.152142544-45
Produced by FastQC (version 0.11.2)