##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	LONLib43_TACAGC_L007_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2822410
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.535589443064616	27.0	15.0	33.0	2.0	33.0
2	24.514735279424322	27.0	15.0	33.0	2.0	33.0
3	25.39007869161461	27.0	22.0	33.0	6.0	33.0
4	27.454964020110474	33.0	15.0	37.0	2.0	37.0
5	27.539333052249674	33.0	15.0	37.0	6.0	37.0
6	28.583919062078152	33.0	22.0	37.0	15.0	37.0
7	28.268703696486337	33.0	22.0	37.0	6.0	37.0
8	28.023441314337745	33.0	22.0	37.0	6.0	37.0
9	28.734599508930312	33.0	22.0	37.0	6.0	37.0
10-11	28.29902583253319	33.0	22.0	37.0	10.5	37.0
12-13	28.552938269067923	33.0	22.0	37.0	10.5	37.0
14-15	28.80865094015398	33.0	22.0	37.0	6.0	40.0
16-17	29.582423000201956	33.0	24.5	37.0	15.0	40.0
18-19	29.83058609486219	33.0	27.0	37.0	15.0	40.0
20-21	29.778573453183625	33.0	27.0	37.0	15.0	40.0
22-23	29.717339968324943	33.0	27.0	37.0	10.5	40.0
24-25	29.270637681981	33.0	24.5	37.0	10.5	40.0
26-27	28.690665778536783	33.0	22.0	37.0	6.0	40.0
28-29	28.775019044008488	33.0	22.0	37.0	10.5	40.0
30-31	28.62161592397986	33.0	22.0	37.0	6.0	40.0
32-33	28.376227939952024	33.0	22.0	37.0	6.0	40.0
34-35	28.36516062513951	33.0	22.0	37.0	6.0	40.0
36-37	28.58573612621837	33.0	22.0	37.0	6.0	40.0
38-39	28.525639790108453	33.0	22.0	37.0	6.0	40.0
40-41	28.513267739272464	33.0	22.0	37.0	6.0	40.0
42-43	28.32236510641615	33.0	22.0	37.0	6.0	40.0
44-45	27.981738655971313	33.0	22.0	37.0	6.0	40.0
46-47	27.56793644438618	33.0	22.0	37.0	6.0	40.0
48-49	27.338615757455507	33.0	22.0	37.0	6.0	40.0
50-51	26.5060292445109	30.0	18.5	35.0	6.0	38.5
52-53	26.21698778703307	27.0	22.0	35.0	6.0	37.0
54-55	27.079047338976267	33.0	22.0	37.0	6.0	38.5
56-57	26.930147462629456	33.0	22.0	37.0	6.0	40.0
58-59	27.065236978327032	33.0	22.0	37.0	6.0	40.0
60-61	26.8076928936618	30.0	22.0	37.0	6.0	38.5
62-63	26.61691497691689	27.0	22.0	37.0	6.0	37.0
64-65	26.542310826563114	27.0	22.0	37.0	6.0	37.0
66-67	26.398543797676453	27.0	22.0	33.0	6.0	37.0
68-69	26.075830230193347	27.0	22.0	33.0	6.0	37.0
70-71	25.967083449959432	27.0	22.0	33.0	6.0	37.0
72-73	25.723163006083453	27.0	15.0	33.0	6.0	37.0
74-75	25.63287899348429	27.0	18.5	33.0	6.0	37.0
76-77	25.267231018881027	27.0	15.0	33.0	6.0	37.0
78-79	24.737632378003195	27.0	15.0	33.0	6.0	37.0
80-81	24.601120673467	27.0	15.0	33.0	6.0	37.0
82-83	24.202038683252965	27.0	15.0	33.0	6.0	37.0
84-85	24.286494697793728	27.0	15.0	33.0	6.0	37.0
86-87	23.988530546589615	27.0	15.0	33.0	6.0	37.0
88-89	23.639692673991377	27.0	15.0	33.0	6.0	37.0
90-91	23.50296129903168	27.0	15.0	33.0	6.0	37.0
92-93	23.384099758716843	27.0	15.0	33.0	6.0	37.0
94-95	23.176023681888882	27.0	15.0	33.0	6.0	37.0
96-97	23.047211071389345	27.0	15.0	33.0	6.0	33.0
98-99	22.80238679001279	27.0	15.0	33.0	6.0	33.0
100-101	22.34108439241641	24.5	15.0	33.0	6.0	33.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	8.282383564442593
2306	2	8.458188999804982
2306	3	7.385767632508678
2306	4	8.967797383376137
2306	5	8.831350701615424
2306	6	7.868538484583887
2306	7	8.025848880916012
2306	8	8.406320480785354
2306	9	7.3605433786366845
2306	10-11	7.750685937276629
2306	12-13	7.335795940257547
2306	14-15	7.820283206912109
2306	16-17	6.601723225702248
2306	18-19	6.025686065564635
2306	20-21	5.876918209472382
2306	22-23	5.442387071601221
2306	24-25	5.575441362098246
2306	26-27	6.2196691842764835
2306	28-29	5.292309070731498
2306	30-31	5.152295943568976
2306	32-33	5.013180508380302
2306	34-35	4.455099513882832
2306	36-37	4.466967065974885
2306	38-39	4.149596583449988
2306	40-41	3.377734890675363
2306	42-43	3.1411542616675234
2306	44-45	2.814161813818103
2306	46-47	3.0557310605295562
2306	48-49	2.929979502110882
2306	50-51	2.7834946448883855
2306	52-53	3.2687268439598753
2306	54-55	2.8539161774114596
2306	56-57	3.0807909086860157
2306	58-59	2.5714976522441333
2306	60-61	2.665267118027977
2306	62-63	2.179925605380312
2306	64-65	1.8451952465949795
2306	66-67	1.6040537297853312
2306	68-69	1.778215573129362
2306	70-71	1.802275287176787
2306	72-73	1.1219609230409233
2306	74-75	0.665581247309369
2306	76-77	1.1345711403428886
2306	78-79	1.2593560769701888
2306	80-81	1.0449999965415486
2306	82-83	1.060531847540613
2306	84-85	0.6127857859545216
2306	86-87	0.6057625503196604
2306	88-89	0.6226496320940633
2306	90-91	0.4885553838557435
2306	92-93	0.4461531706926678
2306	94-95	0.7496713049408328
2306	96-97	0.3233806016031231
2306	98-99	0.22820267016155427
2306	100-101	0.08247737835347735
2307	1	8.32735064868077
2307	2	8.307448504219355
2307	3	7.504808653140696
2307	4	9.034812218070293
2307	5	9.016648542950893
2307	6	7.498525593920611
2307	7	8.107635568571883
2307	8	8.52577458427102
2307	9	7.433684028480371
2307	10-11	7.758827525944987
2307	12-13	7.3223213975789605
2307	14-15	8.151680563662325
2307	16-17	6.816973746481249
2307	18-19	6.307932770985481
2307	20-21	6.365171003028937
2307	22-23	6.035648940462902
2307	24-25	6.140013065227343
2307	26-27	6.731671586656098
2307	28-29	5.920151259678661
2307	30-31	5.841055967972093
2307	32-33	5.869227139833146
2307	34-35	5.489028333903505
2307	36-37	5.46711378814917
2307	38-39	4.867669192212983
2307	40-41	4.387475617015877
2307	42-43	4.23471094044092
2307	44-45	4.198793677590533
2307	46-47	4.463748547324371
2307	48-49	4.201088560662566
2307	50-51	3.7610756382723594
2307	52-53	4.43584993746618
2307	54-55	4.03877567297496
2307	56-57	4.4296585849843275
2307	58-59	4.033212217934992
2307	60-61	3.9651740447838435
2307	62-63	3.6790753526563016
2307	64-65	3.57114175571612
2307	66-67	3.2652306176289727
2307	68-69	3.2742827512500057
2307	70-71	3.011626651001375
2307	72-73	2.4257937174193565
2307	74-75	2.104727062269241
2307	76-77	2.364232942190828
2307	78-79	2.674538736846973
2307	80-81	2.5331290296153597
2307	82-83	2.5587638858016675
2307	84-85	1.7539620795253121
2307	86-87	1.7116284659265943
2307	88-89	1.9786384294185702
2307	90-91	1.7382658655012442
2307	92-93	1.6148157469919298
2307	94-95	1.7669715360118197
2307	96-97	1.63867254446442
2307	98-99	1.4169714189959244
2307	100-101	1.0746096736059343
1101	1	8.004054462972476
1101	2	7.798440111378593
1101	3	7.23115611250666
1101	4	8.685117321064943
1101	5	8.52079806190402
1101	6	7.28750515314259
1101	7	6.966296234897808
1101	8	8.01881167964509
1101	9	7.406427194755608
1101	10-11	7.5430524885288435
1101	12-13	7.265658729503194
1101	14-15	7.660758323430251
1101	16-17	6.913890582963585
1101	18-19	5.968087796150719
1101	20-21	5.811276763701404
1101	22-23	5.234945463114794
1101	24-25	5.869692120491017
1101	26-27	6.454635272609529
1101	28-29	5.846058968245984
1101	30-31	5.6258871619797866
1101	32-33	5.587340663275324
1101	34-35	5.297662547201661
1101	36-37	5.241457936880984
1101	38-39	4.766108556910968
1101	40-41	4.145282138072233
1101	42-43	3.9405337229406143
1101	44-45	3.8414029795671283
1101	46-47	4.113448805974468
1101	48-49	4.056317281738654
1101	50-51	3.483466201217574
1101	52-53	4.354659689146835
1101	54-55	4.226505043385636
1101	56-57	4.305787648070094
1101	58-59	3.8148636169638905
1101	60-61	4.045791831226218
1101	62-63	3.7304292664780725
1101	64-65	3.59851620598549
1101	66-67	3.123485777162017
1101	68-69	3.206017022432146
1101	70-71	3.2025981701414885
1101	72-73	2.765385440097667
1101	74-75	2.2388460131063255
1101	76-77	2.5080273932203703
1101	78-79	2.9047473193468463
1101	80-81	2.632718145621702
1101	82-83	2.791157986706473
1101	84-85	2.089489296950429
1101	86-87	2.192533089992594
1101	88-89	2.075730958288098
1101	90-91	1.9535036365566292
1101	92-93	1.7933724140027287
1101	94-95	1.8434656653997195
1101	96-97	1.8815160969077098
1101	98-99	1.765905859510628
1101	100-101	1.390707021577768
2201	1	6.929832879328217
2201	2	7.69294028946009
2201	3	6.152665474024925
2201	4	8.241509117251077
2201	5	3.6959238647111015
2201	6	5.525339809360837
2201	7	6.537136094974915
2201	8	7.566721702580608
2201	9	6.3722847928551545
2201	10-11	3.802118520814183
2201	12-13	6.280836208009973
2201	14-15	6.793576631657601
2201	16-17	5.829229841640892
2201	18-19	1.6916395394489214
2201	20-21	4.775668532879418
2201	22-23	4.931729597004971
2201	24-25	5.245503869448406
2201	26-27	5.914746112243321
2201	28-29	4.692869685316126
2201	30-31	4.974008554695448
2201	32-33	5.026385877413961
2201	34-35	4.669511650467079
2201	36-37	4.786947957148019
2201	38-39	4.036282549650377
2201	40-41	3.7696889176095283
2201	42-43	3.6691962044618265
2201	44-45	3.5823694300141966
2201	46-47	4.003139792996446
2201	48-49	3.658471017245059
2201	50-51	3.2266882854504644
2201	52-53	4.159755408668296
2201	54-55	3.697934459841953
2201	56-57	3.9232264513582606
2201	58-59	3.571478975686137
2201	60-61	3.4729217351998543
2201	62-63	3.1959194264782127
2201	64-65	2.970003945370326
2201	66-67	2.6410944552342066
2201	68-69	2.8335056859324688
2201	70-71	2.6724720521528376
2201	72-73	2.1729784013241797
2201	74-75	1.8435193502211433
2201	76-77	2.3044245185559014
2201	78-79	2.427595884483406
2201	80-81	2.0403226947892676
2201	82-83	2.420519960146656
2201	84-85	1.934142766937871
2201	86-87	1.813335837374801
2201	88-89	1.8712895807303482
2201	90-91	1.725056294480975
2201	92-93	1.5896520566467913
2201	94-95	1.7056944313254476
2201	96-97	1.5662940810814767
2201	98-99	1.6028856359074233
2201	100-101	1.324144741100163
2304	1	1.0808534210105698
2304	2	4.185450469951697
2304	3	5.481649119223029
2304	4	4.215889052040168
2304	5	6.275571530586085
2304	6	6.179455684232352
2304	7	6.9443770690302
2304	8	6.278005981577998
2304	9	5.675356883365193
2304	10-11	6.552363036510059
2304	12-13	6.5097664464956395
2304	14-15	6.6000888020646045
2304	16-17	5.345017360169248
2304	18-19	5.227802989366104
2304	20-21	4.770541585822702
2304	22-23	4.36808588537507
2304	24-25	4.601342351531116
2304	26-27	5.625583562380285
2304	28-29	4.837686993721412
2304	30-31	4.748393717870634
2304	32-33	4.847724120377965
2304	34-35	4.403785515068638
2304	36-37	4.460533590933572
2304	38-39	4.138434889177596
2304	40-41	3.485365962147487
2304	42-43	2.822939765403653
2304	44-45	2.9980038409570717
2304	46-47	3.959384250220616
2304	48-49	3.6016780383557467
2304	50-51	3.030576511733347
2304	52-53	3.60033752966671
2304	54-55	3.5204200102549095
2304	56-57	4.255534745229852
2304	58-59	3.4330630334470094
2304	60-61	3.486714541003167
2304	62-63	3.144359509330055
2304	64-65	3.1287574404195375
2304	66-67	2.9284503439317398
2304	68-69	3.188086522152336
2304	70-71	3.221079860518028
2304	72-73	2.8049094414146047
2304	74-75	2.2384318315919742
2304	76-77	2.4384998574441425
2304	78-79	2.851707546402057
2304	80-81	2.6099395246581736
2304	82-83	2.7000057198688694
2304	84-85	2.3399984446401305
2304	86-87	2.0656133574664537
2304	88-89	2.332689890616514
2304	90-91	2.1775162901140206
2304	92-93	2.135279719703867
2304	94-95	2.2905777314187823
2304	96-97	2.159019073848121
2304	98-99	2.1033535660335225
2304	100-101	1.6169329716137284
1102	1	6.963450254417246
1102	2	7.292807826830593
1102	3	6.037721686641476
1102	4	5.766275197087058
1102	5	6.434892728827496
1102	6	5.635715508736787
1102	7	6.590789270742075
1102	8	6.363762254523085
1102	9	4.788842157880925
1102	10-11	5.9553597274464884
1102	12-13	5.820916209464784
1102	14-15	6.17227401482517
1102	16-17	5.266149572466805
1102	18-19	5.288137177064208
1102	20-21	5.532861410824243
1102	22-23	5.231650524679335
1102	24-25	1.7312496247792453
1102	26-27	5.375320234592593
1102	28-29	4.937766489954761
1102	30-31	4.938439345952215
1102	32-33	4.919124627484109
1102	34-35	4.8504362851440845
1102	36-37	4.69317124393136
1102	38-39	3.8492293806996436
1102	40-41	4.047034354591688
1102	42-43	4.046094491251186
1102	44-45	-3.747687995347764
1102	46-47	0.9966688719096801
1102	48-49	-0.8796591075677576
1102	50-51	2.3951546105091097
1102	52-53	-0.3036343424166077
1102	54-55	2.447347943282743
1102	56-57	-0.5791954415747398
1102	58-59	2.6888142620975835
1102	60-61	3.6869860382855286
1102	62-63	3.3992466874535587
1102	64-65	2.909588539722037
1102	66-67	2.97406609758535
1102	68-69	3.3826791252664137
1102	70-71	3.2600728864542425
1102	72-73	2.7762690691819962
1102	74-75	2.5552546732919446
1102	76-77	2.7425912726157087
1102	78-79	-1.2668168245827012
1102	80-81	1.4834849483231523
1102	82-83	2.496719316584695
1102	84-85	2.1943540200832388
1102	86-87	2.292559668869906
1102	88-89	2.296620336869289
1102	90-91	2.264876946656198
1102	92-93	2.0856945715026214
1102	94-95	2.276653721978974
1102	96-97	2.281583759835211
1102	98-99	2.20974267249235
1102	100-101	1.8952941630168212
2202	1	-20.22302820026329
2202	2	-7.357998541693993
2202	3	-0.7878408339601464
2202	4	1.209797139463781
2202	5	0.9868430929050405
2202	6	1.5212382962293454
2202	7	3.8380192401447033
2202	8	2.421219948243383
2202	9	2.904548365289262
2202	10-11	1.8262199562746915
2202	12-13	1.2055143840967268
2202	14-15	2.4783635082793722
2202	16-17	1.4529333290939839
2202	18-19	1.6594043467955792
2202	20-21	1.05591009261126
2202	22-23	1.2255683391324226
2202	24-25	2.158990124243047
2202	26-27	3.308830044474046
2202	28-29	-1.2588347427026534
2202	30-31	0.6689659979072253
2202	32-33	1.364212122131537
2202	34-35	1.5295566869386477
2202	36-37	2.8221086570964786
2202	38-39	2.3602592933162434
2202	40-41	1.8300350112764718
2202	42-43	0.5069464212404071
2202	44-45	0.6324031515418582
2202	46-47	-1.2357679976977423
2202	48-49	0.7030805224775669
2202	50-51	1.723530418700328
2202	52-53	1.7218036356993736
2202	54-55	-0.8464179283109949
2202	56-57	0.9723646040936877
2202	58-59	1.9507083518660728
2202	60-61	1.9511302831972905
2202	62-63	-1.970887029279634
2202	64-65	-0.007082705083274021
2202	66-67	0.44532516358345475
2202	68-69	1.513112113639142
2202	70-71	-2.5970611628144056
2202	72-73	-4.501771965829896
2202	74-75	-1.452171452664885
2202	76-77	-0.3725185787513823
2202	78-79	1.5268902585764899
2202	80-81	1.5414309625699758
2202	82-83	-0.7199565191951649
2202	84-85	-0.32134221382866457
2202	86-87	0.7192021210339199
2202	88-89	1.2472763242360188
2202	90-91	1.4093426787322265
2202	92-93	1.3803061579414724
2202	94-95	1.6260960918562901
2202	96-97	1.3646227659187424
2202	98-99	1.172501760910503
2202	100-101	0.9532698495921466
2305	1	5.48882496205427
2305	2	6.928159809181366
2305	3	6.588704975867074
2305	4	7.580576979425082
2305	5	7.613999676995046
2305	6	6.020540843422161
2305	7	2.7659054930345413
2305	8	5.857448890043798
2305	9	6.226842066532896
2305	10-11	4.588821865332065
2305	12-13	5.16512190222128
2305	14-15	5.500650006263431
2305	16-17	5.0222271325281405
2305	18-19	4.326933596155818
2305	20-21	5.100513373853918
2305	22-23	5.225372771144205
2305	24-25	5.170911213196582
2305	26-27	6.05300340217417
2305	28-29	5.151421557831604
2305	30-31	5.3910251194498535
2305	32-33	5.1620998655968755
2305	34-35	5.2754259181287395
2305	36-37	4.729369542755073
2305	38-39	4.649207964844653
2305	40-41	4.145608692827803
2305	42-43	4.050574282307359
2305	44-45	3.124803712682912
2305	46-47	3.8852188977697146
2305	48-49	3.4809899694557167
2305	50-51	2.3745281109063754
2305	52-53	3.8372676076337626
2305	54-55	4.1161476384169156
2305	56-57	4.797619155774328
2305	58-59	4.527795455575994
2305	60-61	4.595469978119379
2305	62-63	3.8681122484585977
2305	64-65	4.146273825394918
2305	66-67	3.806333252120581
2305	68-69	4.246848921205437
2305	70-71	3.887466500504221
2305	72-73	-0.6063486744822946
2305	74-75	2.1029402330089084
2305	76-77	3.0542895540241517
2305	78-79	3.7229533053352206
2305	80-81	3.3261991709481507
2305	82-83	3.620453711022847
2305	84-85	3.0023897959795853
2305	86-87	2.9563080316700976
2305	88-89	3.0210447375046456
2305	90-91	2.8871268124271694
2305	92-93	2.87957230676815
2305	94-95	3.0724680025672555
2305	96-97	3.0296200236877446
2305	98-99	3.122336534233497
2305	100-101	2.7055730587308915
1103	1	-20.22302820026329
1103	2	-7.407830136562435
1103	3	-0.838100243354539
1103	4	2.58513482132161
1103	5	4.386738892535355
1103	6	4.290421281266916
1103	7	3.7528953906089946
1103	8	4.087933371486191
1103	9	4.0734788973986475
1103	10-11	4.672452972803715
1103	12-13	4.398961816509036
1103	14-15	3.8837386247228025
1103	16-17	2.505151464324431
1103	18-19	2.966388231579554
1103	20-21	2.245970931551309
1103	22-23	2.5238738286802906
1103	24-25	2.8934375348247627
1103	26-27	4.088456770598135
1103	28-29	3.4387699521137556
1103	30-31	3.2854517801610363
1103	32-33	2.874039148866199
1103	34-35	2.2545766195765964
1103	36-37	2.420314654515252
1103	38-39	2.109031811006357
1103	40-41	2.284972098429755
1103	42-43	2.124492698032171
1103	44-45	2.5142543339944865
1103	46-47	1.7589582840922482
1103	48-49	-1.1842516873946245
1103	50-51	-1.9479120167861836
1103	52-53	1.5030529942219601
1103	54-55	2.287028028092326
1103	56-57	3.0215259670658057
1103	58-59	2.5996703232927523
1103	60-61	2.810124260885118
1103	62-63	2.7170787240535255
1103	64-65	2.549433369566941
1103	66-67	2.453450462735937
1103	68-69	2.751063009133464
1103	70-71	2.687945420576682
1103	72-73	2.1209376543274985
1103	74-75	1.9336574599726504
1103	76-77	2.2768472587110296
1103	78-79	2.7248154884575584
1103	80-81	2.4263447007598664
1103	82-83	2.5318425500394355
1103	84-85	2.11133352980454
1103	86-87	1.9733749418109134
1103	88-89	2.0664027452205005
1103	90-91	-2.3456227230305764
1103	92-93	-2.954151125283147
1103	94-95	-3.0706445837908944
1103	96-97	0.211360411348803
1103	98-99	-1.2742340272897046
1103	100-101	0.7220655859137324
2203	1	2.5719639750105756
2203	2	3.1694656882581427
2203	3	-5.624579662590893
2203	4	-3.1037306782397494
2203	5	0.031858971276964354
2203	6	0.2794917510234818
2203	7	2.3880886550729343
2203	8	-7.507894990692748
2203	9	-6.948750186676762
2203	10-11	-11.893925496029086
2203	12-13	-9.740395596764145
2203	14-15	-1.34579663597966
2203	16-17	-1.193056485243165
2203	18-19	-0.48713433395667494
2203	20-21	-1.30171359880649
2203	22-23	-2.9940566363984082
2203	24-25	-0.7551878072533782
2203	26-27	-1.5023858649732702
2203	28-29	0.5704590599916806
2203	30-31	-1.9374153086940602
2203	32-33	0.023911429856873667
2203	34-35	0.1686774696347726
2203	36-37	-0.1950766297506661
2203	38-39	0.6930395243196052
2203	40-41	-0.3722619180637814
2203	42-43	0.46659131097864304
2203	44-45	0.331430585489521
2203	46-47	0.5737732833654796
2203	48-49	0.7717706586360897
2203	50-51	0.6427520630356156
2203	52-53	1.4483302684282506
2203	54-55	0.5958422260781866
2203	56-57	0.6218973556466452
2203	58-59	0.952024639878001
2203	60-61	0.5716750470837901
2203	62-63	0.8869896453938146
2203	64-65	0.570328819272877
2203	66-67	-0.24627779312117326
2203	68-69	0.7558052506279047
2203	70-71	0.6995524665931576
2203	72-73	0.37073069198136466
2203	74-75	0.05716270117416755
2203	76-77	0.12644263072307993
2203	78-79	0.8340558222830268
2203	80-81	0.7266745493047466
2203	82-83	0.2466559998619502
2203	84-85	0.2543234827233718
2203	86-87	0.2962716177964211
2203	88-89	0.5261020853758467
2203	90-91	0.1260505509366574
2203	92-93	0.3741075724846823
2203	94-95	0.8136879976899216
2203	96-97	0.46230557628782876
2203	98-99	0.27736895326321687
2203	100-101	-0.24918011343822855
2310	1	-20.22302820026329
2310	2	-19.960163941371487
2310	3	-20.866624524354066
2310	4	-22.792045753878767
2310	5	-22.99657086701203
2310	6	-24.043147789925918
2310	7	-23.615562883789874
2310	8	-23.227248146665623
2310	9	-24.131848778226058
2310	10-11	-23.787117984291996
2310	12-13	-24.032909942095394
2310	14-15	-24.28528542053887
2310	16-17	-25.27443363704285
2310	18-19	-19.43966547320796
2310	20-21	-19.49045784985877
2310	22-23	-25.682632536241915
2310	24-25	-25.250362559470382
2310	26-27	-18.181208152704826
2310	28-29	-18.584475724801457
2310	30-31	-19.087563299821227
2310	32-33	-17.43153587049244
2310	34-35	-9.872884792959695
2310	36-37	-14.307301937457993
2310	38-39	-18.841609294601103
2310	40-41	-11.145614325096947
2310	42-43	-4.825124825307327
2310	44-45	-13.36943701263657
2310	46-47	-18.075019371806608
2310	48-49	-18.40964785422876
2310	50-51	-10.395920647336444
2310	52-53	-14.105529529234424
2310	54-55	-17.661794979012498
2310	56-57	-17.177259595060896
2310	58-59	-9.981521095228526
2310	60-61	-7.314779101073974
2310	62-63	-4.453901924754163
2310	64-65	-14.45679558185575
2310	66-67	-9.971785725083937
2310	68-69	-5.228042589635525
2310	70-71	-2.7756805069881807
2310	72-73	-3.692405646403877
2310	74-75	-3.629519562299251
2310	76-77	-2.761039249490974
2310	78-79	-3.168905888632196
2310	80-81	-2.7139197911190216
2310	82-83	-11.799523732392107
2310	84-85	-9.589493404264786
2310	86-87	-5.75704707550522
2310	88-89	-4.195913445967349
2310	90-91	-1.7215223075981996
2310	92-93	-1.2319227311906857
2310	94-95	-1.9572293824951785
2310	96-97	-0.9768677357309343
2310	98-99	-0.8929497100533652
2310	100-101	-0.9442801090205855
2204	1	-20.22302820026329
2204	2	-19.960163941371487
2204	3	-20.866624524354066
2204	4	-22.792045753878767
2204	5	-22.99657086701203
2204	6	-24.043147789925918
2204	7	-23.615562883789874
2204	8	-23.227248146665623
2204	9	-24.131848778226058
2204	10-11	-17.80587091443733
2204	12-13	-18.302717724561404
2204	14-15	-18.272158369437136
2204	16-17	-19.454696178064886
2204	18-19	-25.635804130513797
2204	20-21	-13.3917276635041
2204	22-23	-6.35304978893765
2204	24-25	-15.203245822480227
2204	26-27	-24.30911512944901
2204	28-29	-12.163756123278594
2204	30-31	-14.861931463286876
2204	32-33	-18.21080559238912
2204	34-35	-18.430452235931018
2204	36-37	-24.608091757554526
2204	38-39	-12.422332265726816
2204	40-41	-6.783306007259057
2204	42-43	-4.48815734032911
2204	44-45	-13.17308266371975
2204	46-47	-18.95368754348026
2204	48-49	-17.385975159922708
2204	50-51	-9.548138238926178
2204	52-53	-2.395366700068145
2204	54-55	-23.574475979451286
2204	56-57	-17.408003333061316
2204	58-59	-10.361933416948663
2204	60-61	-14.402470340949991
2204	62-63	-11.012809288858676
2204	64-65	-4.724133453661494
2204	66-67	-2.751849196223329
2204	68-69	-6.0168237252904255
2204	70-71	-6.1616312791065475
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2204	74-75	-2.4992941835943583
2204	76-77	-2.7322996023283004
2204	78-79	-15.957933573067038
2204	80-81	-6.638855716207686
2204	82-83	-3.2009777633360876
2204	84-85	-2.070683285499655
2204	86-87	-1.7694890974561872
2204	88-89	-2.6337501362520968
2204	90-91	-1.9720243023084585
2204	92-93	-1.508093981895513
2204	94-95	-1.040164606920392
2204	96-97	-1.0900325971503584
2204	98-99	-1.2852354589578887
2204	100-101	-1.9046924183978504
2311	1	8.232520349547556
2311	2	8.438480447027
2311	3	7.469598678672416
2311	4	8.960633943473063
2311	5	8.803870494904746
2311	6	7.9161838620286815
2311	7	8.149884783297136
2311	8	8.21868501852984
2311	9	7.467142394535607
2311	10-11	7.645653138028308
2311	12-13	7.340750209228695
2311	14-15	7.863595411743603
2311	16-17	6.701055013650713
2311	18-19	5.999126512613564
2311	20-21	6.088929645320366
2311	22-23	5.576352331349508
2311	24-25	5.565841728045378
2311	26-27	6.298151579252121
2311	28-29	5.2095057425906575
2311	30-31	4.780361678059496
2311	32-33	5.032430316752578
2311	34-35	4.661304951164933
2311	36-37	4.624174951146607
2311	38-39	3.88382021918423
2311	40-41	3.1378988374646752
2311	42-43	2.931917273808068
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2311	46-47	2.8488778597284288
2311	48-49	2.7632314616588864
2311	50-51	2.392010457266302
2311	52-53	3.065861749876845
2311	54-55	2.623427551444049
2311	56-57	2.8373891704371204
2311	58-59	2.21212125830699
2311	60-61	2.3424194929754307
2311	62-63	1.975149624370001
2311	64-65	1.6643660178038395
2311	66-67	1.2208150234579094
2311	68-69	1.2798979409062348
2311	70-71	1.2110485483097406
2311	72-73	0.4649905202518134
2311	74-75	0.183942916819543
2311	76-77	0.6021539111561331
2311	78-79	0.8099749726999832
2311	80-81	0.5213705427423747
2311	82-83	0.5900768244216543
2311	84-85	0.009210729702516574
2311	86-87	-0.1412734015137822
2311	88-89	-0.21427130601636435
2311	90-91	-0.28410558343155046
2311	92-93	-0.3395987641291782
2311	94-95	-0.11691549623613184
2311	96-97	-0.08208543701913484
2311	98-99	-0.3670861313615781
2311	100-101	-0.658581197914355
2205	1	8.394802223676859
2205	2	8.478271220723276
2205	3	7.584591186369128
2205	4	9.18535449549779
2205	5	9.149158559421888
2205	6	7.861933257455625
2205	7	8.153140358105386
2205	8	8.538026167548843
2205	9	7.604279650701621
2205	10-11	7.821516679049651
2205	12-13	7.497404895809844
2205	14-15	7.935724554523475
2205	16-17	6.882595664702787
2205	18-19	6.274809959261766
2205	20-21	6.199830573170974
2205	22-23	5.779960979967559
2205	24-25	5.7517415552428375
2205	26-27	6.457250207209341
2205	28-29	5.711590890691404
2205	30-31	5.477393432689308
2205	32-33	5.370219100625182
2205	34-35	5.141856115027032
2205	36-37	5.008257993068916
2205	38-39	4.443018751070152
2205	40-41	3.98511923618414
2205	42-43	3.7748365754908093
2205	44-45	3.7756287591997477
2205	46-47	4.036157237733608
2205	48-49	3.5735687764739943
2205	50-51	3.393152974554418
2205	52-53	4.028923240224117
2205	54-55	3.679498459451459
2205	56-57	3.8988202593290175
2205	58-59	3.431307490393859
2205	60-61	3.549852321685499
2205	62-63	3.0701951521017428
2205	64-65	2.941815619681872
2205	66-67	2.577318252302568
2205	68-69	2.4560216095090013
2205	70-71	2.491122421791232
2205	72-73	1.8860267144447675
2205	74-75	1.5305863570839477
2205	76-77	1.9002393839495575
2205	78-79	2.026394241363132
2205	80-81	1.787458947204108
2205	82-83	1.8761074917633884
2205	84-85	1.2864709602736255
2205	86-87	1.080060873140063
2205	88-89	1.1760266918955367
2205	90-91	0.9256642660619896
2205	92-93	0.6389535349780324
2205	94-95	0.903410914250891
2205	96-97	0.6513778389020324
2205	98-99	0.5300287422362686
2205	100-101	0.1333698041517266
2206	1	8.109986153803696
2206	2	8.316231592918783
2206	3	7.277713593668263
2206	4	8.805083432724103
2206	5	8.800239340324492
2206	6	7.557809147873122
2206	7	8.118089428809807
2206	8	8.579450417927681
2206	9	7.403717409971712
2206	10-11	7.864636401672918
2206	12-13	7.544442529993923
2206	14-15	8.119260034006587
2206	16-17	6.890398898842317
2206	18-19	6.423366523393703
2206	20-21	6.19183593158515
2206	22-23	5.808117065034008
2206	24-25	6.20043488869549
2206	26-27	6.717120915208085
2206	28-29	5.722375681107707
2206	30-31	5.533175951074366
2206	32-33	5.515334556602461
2206	34-35	5.13824539998847
2206	36-37	5.312083681041965
2206	38-39	4.780558399755456
2206	40-41	4.224814729309379
2206	42-43	3.892116640877582
2206	44-45	3.8897441386140486
2206	46-47	4.119814745678259
2206	48-49	3.974851628952049
2206	50-51	3.8920404747035704
2206	52-53	4.322768123629636
2206	54-55	3.9780758546794956
2206	56-57	4.17360993486108
2206	58-59	3.826419689958744
2206	60-61	3.94254622488603
2206	62-63	3.410365609133013
2206	64-65	3.1684448947796113
2206	66-67	2.874813275708963
2206	68-69	2.9968355187152547
2206	70-71	2.958063447416958
2206	72-73	2.2934167819395057
2206	74-75	1.9324438047725465
2206	76-77	2.23442572332441
2206	78-79	2.6551052091589504
2206	80-81	2.1869506485726937
2206	82-83	2.2732112733779317
2206	84-85	1.5985144659432642
2206	86-87	1.5338374166298223
2206	88-89	1.6810342835623047
2206	90-91	1.5681951249196295
2206	92-93	1.2908793176107665
2206	94-95	1.6323985776681056
2206	96-97	1.321608015056544
2206	98-99	1.0739696276622546
2206	100-101	0.75813494472931
2309	1	8.020294219174932
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2309	16-17	6.691101431025391
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2309	22-23	5.685711423778766
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2309	28-29	5.628461297605355
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2309	36-37	5.415947532467875
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2207	1	6.877435754055053
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2207	9	5.629036303858168
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2207	36-37	3.7360374358787354
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2104	7	-23.615562883789874
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2104	9	-24.131848778226058
2104	10-11	-12.168070365244377
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2104	18-19	-4.018661273370938
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2104	28-29	-1.7803986790350734
2104	30-31	-3.5266022823880725
2104	32-33	-1.5305263076794553
2104	34-35	-3.597992923091958
2104	36-37	-3.694599694062461
2104	38-39	-2.304821717203609
2104	40-41	-1.9002707115655326
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2104	48-49	-5.153178294341032
2104	50-51	-3.1023690339164673
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2104	96-97	-3.05172303985346
2104	98-99	-2.151087712658242
2104	100-101	-1.2609157122160823
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2110	3	-20.866624524354066
2110	4	-22.792045753878767
2110	5	-11.564049199657509
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2110	9	-14.361316380372157
2110	10-11	-23.787117984291996
2110	12-13	-24.032909942095394
2110	14-15	-24.28528542053887
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2110	20-21	-25.54245433954723
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2110	26-27	-18.52682045342755
2110	28-29	-10.72063340052031
2110	30-31	-18.84951432242764
2110	32-33	-18.640030791822817
2110	34-35	-18.707240608959538
2110	36-37	-24.608091757554526
2110	38-39	-14.34610891856097
2110	40-41	-17.3668862806161
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2110	46-47	-3.734239272795687
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2110	66-67	-2.651156777364289
2110	68-69	-1.3550907316459515
2110	70-71	-0.788682187352439
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2110	74-75	-2.1710931394903383
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2110	78-79	-1.4530367596526972
2110	80-81	-1.2975647708429179
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2110	84-85	-1.6503614711323813
2110	86-87	-1.7505126877986967
2110	88-89	-1.8776466785886896
2110	90-91	-1.7236870962976916
2110	92-93	-1.4245443765061552
2110	94-95	-1.3935340878229567
2110	96-97	-1.2945939397610928
2110	98-99	-1.669671784614163
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2109	1	7.614709206838278
2109	2	7.632115166803576
2109	3	6.7989411238705415
2109	4	8.415633849754592
2109	5	7.96482467386328
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2109	8	7.679234099412
2109	9	7.1658390830456185
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2109	14-15	6.998674117033389
2109	16-17	2.3029817221644144
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2109	22-23	1.628577207771297
2109	24-25	1.0681758385477842
2109	26-27	4.855211873028281
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2109	30-31	4.077414141137023
2109	32-33	4.3488662971466745
2109	34-35	4.086813022407629
2109	36-37	4.14035993526133
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2109	44-45	2.810857187921499
2109	46-47	2.984834329582842
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2109	52-53	3.3060793641587054
2109	54-55	2.6039922286412
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2109	58-59	2.364273125439194
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2109	64-65	1.6906672596776708
2109	66-67	1.5445801785151119
2109	68-69	1.4316429787737022
2109	70-71	1.2842405889044137
2109	72-73	1.0303625357091128
2109	74-75	0.5523698679599391
2109	76-77	0.8503974708751052
2109	78-79	1.3708663342522946
2109	80-81	1.0719175982643137
2109	82-83	0.9905346000867183
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2109	98-99	0.4167657601374266
2109	100-101	0.26635330328992524
2108	1	6.360276623547865
2108	2	6.87613438226861
2108	3	5.827012150295268
2108	4	7.60720159473567
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2108	6	3.841559702376511
2108	7	6.3002770067335625
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2108	64-65	2.197997538089748
2108	66-67	1.953118429630372
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2108	76-77	1.368541745348491
2108	78-79	1.848059128495283
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2211	1	6.441487928768968
2211	2	6.150409535331022
2211	3	5.552192679947009
2211	4	7.51225532139005
2211	5	7.142318021876857
2211	6	6.986601314016731
2211	7	7.0487740337728475
2211	8	6.619526046882765
2211	9	5.437685271953153
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2211	12-13	5.540118731739732
2211	14-15	6.426900959389442
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2211	22-23	3.9974749906398053
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2211	26-27	4.662659064099373
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2211	30-31	4.127506780591954
2211	32-33	4.81018029754328
2211	34-35	4.265473538761601
2211	36-37	4.575062364309275
2211	38-39	4.127346741577753
2211	40-41	3.4702234000576127
2211	42-43	3.2405093112142254
2211	44-45	3.4722714490700497
2211	46-47	3.8639402108563523
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2211	54-55	3.138427246355164
2211	56-57	3.405309356925528
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2211	60-61	0.3849194477770794
2211	62-63	0.6694933894832005
2211	64-65	2.270824805945601
2211	66-67	2.393828555844788
2211	68-69	2.709823205429597
2211	70-71	2.4976404569005943
2211	72-73	2.1014667210590545
2211	74-75	1.682327188696675
2211	76-77	1.8756790888986572
2211	78-79	2.3028633508474847
2211	80-81	2.199739401981141
2211	82-83	2.3928795177915063
2211	84-85	1.8113392331398472
2211	86-87	1.8238260122635808
2211	88-89	1.783090070635069
2211	90-91	1.7028048040539296
2211	92-93	1.5110903412598198
2211	94-95	1.5775028461594722
2211	96-97	1.2995058387947758
2211	98-99	1.3134514578486716
2211	100-101	1.1337514664833535
2210	1	7.118549886408829
2210	2	7.251405968685749
2210	3	6.6513640283851
2210	4	8.020505349964239
2210	5	8.21581670453335
2210	6	7.229133485626001
2210	7	7.56514030508993
2210	8	7.153782106809437
2210	9	6.821748932321775
2210	10-11	6.89562935830817
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2210	14-15	4.5679443423393025
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2210	26-27	4.344911853380097
2210	28-29	4.64253328633761
2210	30-31	4.972449295179448
2210	32-33	5.001526347124628
2210	34-35	4.7744431240987915
2210	36-37	4.6249417665664865
2210	38-39	3.91046401006086
2210	40-41	3.7780359504287837
2210	42-43	3.9704121296552763
2210	44-45	4.099676443545707
2210	46-47	4.097439347769228
2210	48-49	3.878299271883062
2210	50-51	3.9351428740901255
2210	52-53	4.336565443995145
2210	54-55	3.996149531587143
2210	56-57	4.079343182907234
2210	58-59	3.6314227480387906
2210	60-61	3.676908709742335
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2210	64-65	3.139593800456428
2210	66-67	2.97743158558832
2210	68-69	3.085408829826971
2210	70-71	3.122913816668717
2210	72-73	2.615182845988354
2210	74-75	2.458514263197653
2210	76-77	2.6381997136037576
2210	78-79	3.0720671859436273
2210	80-81	2.7246613724574082
2210	82-83	2.76436122884342
2210	84-85	2.1514804775678797
2210	86-87	2.243648735047028
2210	88-89	2.456572707783863
2210	90-91	2.2347385881268202
2210	92-93	2.158684907181211
2210	94-95	2.393795044379374
2210	96-97	2.26671717351725
2210	98-99	2.0787583593219274
2210	100-101	1.8748722928196742
2209	1	4.91277679768837
2209	2	2.2852272917297007
2209	3	4.356234959464043
2209	4	1.795623234240857
2209	5	4.658488534872443
2209	6	4.9902401658299205
2209	7	6.019217943739829
2209	8	6.793849764026717
2209	9	6.0461520411102825
2209	10-11	6.018596886662529
2209	12-13	5.428786084123367
2209	14-15	4.722805525794598
2209	16-17	4.384005527234084
2209	18-19	3.557764079236307
2209	20-21	3.711130256929625
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2209	24-25	4.012234736719705
2209	26-27	1.4625972834965033
2209	28-29	0.023359668766701702
2209	30-31	2.8243393041331935
2209	32-33	4.139073062862149
2209	34-35	4.001767128409185
2209	36-37	3.74493978689447
2209	38-39	-1.0767139289565328
2209	40-41	-0.21744848804277694
2209	42-43	-4.277337500789201
2209	44-45	0.35657506457518195
2209	46-47	2.2826665980382934
2209	48-49	1.455944495564225
2209	50-51	2.61919128159305
2209	52-53	3.4334999922195877
2209	54-55	2.851169247914548
2209	56-57	3.2837982907457643
2209	58-59	2.5554176625260006
2209	60-61	2.7121940605378754
2209	62-63	2.6545919668040305
2209	64-65	2.007928150981641
2209	66-67	2.0572216214401493
2209	68-69	-0.8841537547738589
2209	70-71	1.482460232273219
2209	72-73	1.7541640702563868
2209	74-75	1.541384012167871
2209	76-77	2.000789581836653
2209	78-79	2.3010069593632956
2209	80-81	1.9304346705340443
2209	82-83	1.9552403003604937
2209	84-85	1.2751189506009624
2209	86-87	1.3396087721348948
2209	88-89	1.616711676739861
2209	90-91	1.5555563718789713
2209	92-93	1.543451079778464
2209	94-95	1.5727533751306453
2209	96-97	1.3269956741786508
2209	98-99	1.0926874120304433
2209	100-101	1.153277404058148
2208	1	4.5418261241230375
2208	2	6.156605959982034
2208	3	4.497908625450936
2208	4	2.8232329797757423
2208	5	3.662295845536722
2208	6	4.824023579654263
2208	7	3.0818447785179934
2208	8	3.809686930186867
2208	9	3.7651459453343925
2208	10-11	4.05126237817646
2208	12-13	4.781726442396462
2208	14-15	5.498954083934635
2208	16-17	3.9685119926887396
2208	18-19	1.6939733413834261
2208	20-21	4.125129968780367
2208	22-23	3.2381061652951324
2208	24-25	3.8290134442001857
2208	26-27	4.939909876286247
2208	28-29	4.152217036670802
2208	30-31	4.2277625238693695
2208	32-33	3.3081135070443217
2208	34-35	3.585228982325404
2208	36-37	3.526113335356694
2208	38-39	3.759233494236323
2208	40-41	3.06081800471933
2208	42-43	3.035293986105131
2208	44-45	-1.5414925591492548
2208	46-47	2.4519573401673576
2208	48-49	2.6172210982675814
2208	50-51	2.8807603426334154
2208	52-53	1.3912103061678813
2208	54-55	0.5511262228887013
2208	56-57	-1.4073426891440306
2208	58-59	1.5605168282559028
2208	60-61	2.743374023281472
2208	62-63	2.020505878783549
2208	64-65	2.549206636147357
2208	66-67	2.6094488150045514
2208	68-69	1.7786384979772087
2208	70-71	-0.2194723883559142
2208	72-73	-0.33831847185537
2208	74-75	-1.9664026669721046
2208	76-77	0.3481279464101199
2208	78-79	1.548450715513841
2208	80-81	1.960780518247823
2208	82-83	2.139127855799387
2208	84-85	1.2882988421060944
2208	86-87	1.8281219689666806
2208	88-89	2.1067005342167846
2208	90-91	1.7012075032031362
2208	92-93	1.5295696585541592
2208	94-95	1.5302264781456998
2208	96-97	1.7493289707649637
2208	98-99	1.9201525237437274
2208	100-101	1.8092634463939063
1307	1	7.122127714994981
1307	2	6.883682761794425
1307	3	6.669438317826369
1307	4	8.341731918104095
1307	5	4.485457221538315
1307	6	5.315576323380437
1307	7	3.5194049810042216
1307	8	5.180749949025881
1307	9	5.566794401969133
1307	10-11	6.600646833608508
1307	12-13	5.634669205726553
1307	14-15	5.1097395735101685
1307	16-17	3.8992154941163975
1307	18-19	4.9143029868392105
1307	20-21	4.9964649653801665
1307	22-23	4.5564771995352835
1307	24-25	5.199768361738855
1307	26-27	5.883339048127752
1307	28-29	5.310560654154685
1307	30-31	4.754300601656919
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1212	46-47	-16.13944942490143
1212	48-49	-18.366930366697034
1212	50-51	-10.900123174524797
1212	52-53	-4.2999109888361176
1212	54-55	-3.2409702917573853
1212	56-57	-3.2406559757554554
1212	58-59	-2.1933495201305035
1212	60-61	-12.941229951947896
1212	62-63	-10.364039355992034
1212	64-65	-14.573687004961279
1212	66-67	-10.882216967945674
1212	68-69	-6.883082559360449
1212	70-71	-13.695908949496165
1212	72-73	-10.144654564519145
1212	74-75	-7.191671951384794
1212	76-77	-16.88401677051949
1212	78-79	-8.60325884283434
1212	80-81	-6.382932035009439
1212	82-83	-4.654600865317022
1212	84-85	-3.851686586655326
1212	86-87	-2.5612285480845394
1212	88-89	-2.4879565280535516
1212	90-91	-2.8862800684223764
1212	92-93	-2.886383926048822
1212	94-95	-2.7199391742042884
1212	96-97	-11.575828028281096
1212	98-99	-8.672129061290075
1212	100-101	-5.353723282676185
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	484.0
3	1052.0
4	662.0
5	3393.0
6	4062.0
7	6519.0
8	13938.0
9	23229.0
10	29718.0
11	35218.0
12	42644.0
13	50423.0
14	55583.0
15	60400.0
16	64982.0
17	69958.0
18	76370.0
19	82072.0
20	87523.0
21	95173.0
22	99755.0
23	105233.0
24	107660.0
25	109750.0
26	111969.0
27	114688.0
28	114206.0
29	114721.0
30	117469.0
31	122086.0
32	128912.0
33	140894.0
34	150909.0
35	155853.0
36	153171.0
37	129518.0
38	42104.0
39	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	57.75205857426178	23.4582412267594	7.284006592016562	11.505693606962256
2	29.087142012685884	23.74121398024288	26.251629940717535	20.9200140663537
3	21.87520404879733	24.601745621577113	29.965995451202478	23.557054878423084
4	26.862591577652257	20.642701350995072	26.40128787939313	26.093419191959544
5	23.288624963993453	26.078767742334254	25.435295315864376	25.197311977807917
6	22.716254544300277	24.782200346925933	25.45879927776668	27.042745831007114
7	27.724246527443697	25.480627449876053	26.090774190167092	20.704351832513154
8	21.7454145811671	23.635748910885805	27.68772045565706	26.931116052290033
9	25.979672345287657	24.935938967715032	23.79001432545919	25.294374361538125
10-11	26.162294790681496	25.388010045386828	23.47292427216404	24.976770891767632
12-13	25.44362227131016	24.85308491781424	24.333015988784833	25.370276822090766
14-15	24.860471721671633	25.43534080966242	24.793543135673833	24.910644332992113
16-17	25.49398204521383	25.586588339751298	24.278410807630877	24.641018807403995
18-19	25.75091729546196	25.610929456095494	23.783329709968342	24.854823538474204
20-21	25.07797692233557	25.749633479260183	24.38040107006533	24.791988528338916
22-23	25.399739978600657	25.051023446127385	24.451198863767125	25.098037711504833
24-25	25.5182705737185	24.93378155215345	24.44788943072386	25.100058443404187
26-27	25.097708231452813	25.17846693432162	24.64739711261753	25.076427721608034
28-29	25.743494741940037	25.122999163459745	24.291251497007067	24.84225459759315
30-31	25.679362707497432	24.856876940147288	24.202085433735785	25.26167491861949
32-33	25.086421465223392	25.25519514171223	24.592709648693827	25.06567374437056
34-35	25.705307638429076	25.032429286815777	24.327672521819103	24.934590552936047
36-37	25.738002800453796	24.849866182695298	24.218888024190782	25.193242992660124
38-39	25.148892726493806	25.101868300725062	24.739552192444275	25.00968678033686
40-41	25.847962812908854	25.041934367944318	24.173185759710293	24.936917059436535
42-43	25.66748187771763	24.926525029757787	24.228154365386604	25.17783872713798
44-45	25.207488974457764	24.99567731303213	24.722917559005573	25.073916153504534
46-47	25.83565854625247	25.015258087863607	24.181717723220437	24.96736564266348
48-49	25.663747123732577	24.788780835595137	24.297161461172543	25.250310579499747
50-51	25.386609507261	25.038856605586968	24.471808338477334	25.102725548674705
52-53	25.59371384440738	25.052169299265604	24.40156823635469	24.95254861997233
54-55	25.52984778077837	24.767228849655922	24.301046783104056	25.401876586461654
56-57	25.140677128616435	25.031452368432294	24.69916321106734	25.128707291883927
58-59	25.731879176825345	24.97268285400548	24.262535984437996	25.032901984731176
60-61	25.641333160017837	24.581744707713234	24.30548723078945	25.47143490147948
62-63	25.220465813832355	25.009547118213725	24.64024337978855	25.12974368816537
64-65	25.8055302956414	24.89083055553962	24.24038577387134	25.063253374947646
66-67	25.777362868375338	24.679194816886195	24.19802934658716	25.345412968151308
68-69	25.356661771860367	24.9405347985021	24.529228242288447	25.173575187349083
70-71	25.971957230340024	24.822487736833576	24.079882396672247	25.12567263615415
72-73	25.7795813982022	24.768715113118514	24.136198952697637	25.31550453598165
74-75	25.451077297185908	24.903144870021002	24.38239891438549	25.263378918407597
76-77	26.062692150482086	24.925479823454225	23.96797718848911	25.043850837574578
78-79	25.94405775536142	24.69388247251967	24.017134400184347	25.34492537193456
80-81	25.692648718118633	24.852279888194808	24.19982237521539	25.255249018471165
82-83	25.998531032734544	24.85014824962902	24.05967937635468	25.091641341281758
84-85	26.05922096561094	24.537968296097382	23.873370880989555	25.529439857302123
86-87	25.70230666705443	24.867976135334303	24.201348718206987	25.228368479404278
88-89	26.150114153733174	24.75709642262749	23.907144443965738	25.1856449796736
90-91	26.224539968023524	24.556440323552145	23.70193754320876	25.51708216521557
92-93	25.892486718857853	24.807849759219962	23.98626263744727	25.313400884474913
94-95	26.33455414684246	24.72320842815056	23.654369386032762	25.287868038974214
96-97	26.33705703612697	24.586430739305836	23.651937208520838	25.424575016046358
98-99	26.100753577766	24.769202405918005	23.797014406790176	25.33302960952582
100-101	26.8523940387039	24.5371877906281	23.226633722749092	25.383784447918913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	185.0
1	191.5
2	222.0
3	236.5
4	223.5
5	246.0
6	294.0
7	369.0
8	498.0
9	636.0
10	773.5
11	997.0
12	1277.5
13	1693.0
14	2171.0
15	2778.5
16	3511.5
17	4347.0
18	5388.5
19	6742.5
20	8458.5
21	10401.0
22	12735.5
23	15349.0
24	18393.0
25	21897.5
26	25464.0
27	28980.5
28	32827.0
29	36987.5
30	40918.0
31	44644.5
32	48487.5
33	51965.0
34	55494.5
35	58975.0
36	61587.0
37	64134.0
38	65929.0
39	66686.0
40	66454.0
41	65833.0
42	64730.5
43	62849.0
44	61187.5
45	59380.0
46	57538.5
47	55991.5
48	54368.0
49	52354.5
50	50608.5
51	49390.0
52	48756.5
53	48763.0
54	49195.5
55	49963.0
56	51462.0
57	52998.5
58	54606.0
59	56686.5
60	58230.0
61	59400.5
62	60491.0
63	61079.5
64	61211.5
65	60652.0
66	59272.5
67	57563.5
68	55026.0
69	51621.0
70	47417.5
71	42837.0
72	38336.0
73	33668.0
74	29029.0
75	24705.5
76	20577.5
77	16787.0
78	13647.5
79	10926.5
80	8663.0
81	6935.0
82	5618.0
83	4590.5
84	3761.5
85	3056.5
86	2449.5
87	1979.0
88	1579.5
89	1228.0
90	1002.0
91	818.5
92	601.0
93	446.5
94	345.5
95	272.5
96	203.5
97	127.0
98	71.0
99	42.5
100	19.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	13.336864594442337
2	10.632013066847128
3	9.382442664247929
4	11.304877746323177
5	8.856296569244016
6	5.123458321080212
7	9.412098171420878
8	9.667659907667561
9	5.174868286322681
10-11	6.081363090408551
12-13	5.17408881062638
14-15	7.067187261949893
16-17	2.5530309203836437
18-19	2.949376596596526
20-21	2.9456386563256225
22-23	3.0095910941358626
24-25	3.33507534341219
26-27	4.182861455281125
28-29	1.6755007245580904
30-31	2.144922247299294
32-33	2.9177192541126202
34-35	1.9160575536509579
36-37	2.9355763337006318
38-39	1.3352772984789594
40-41	1.1139947775128347
42-43	0.5371827622492834
44-45	1.2327053829882972
46-47	1.3110072597531897
48-49	1.2072661307180743
50-51	1.5334944249772358
52-53	2.372369712408899
54-55	1.6991684411548995
56-57	1.7280090419180771
58-59	1.5080197419935446
60-61	1.3289352007681379
62-63	0.5877778210819832
64-65	1.1913931710842862
66-67	0.5446232120776215
68-69	0.7731867446614773
70-71	0.9655223727240196
72-73	0.08514000446426989
74-75	0.1060441254105534
76-77	0.4464447050570257
78-79	0.827750043402624
80-81	0.28523495877636484
82-83	0.4830623474264902
84-85	0.06751322451380204
86-87	0.10076494910378012
88-89	0.08182723275498599
90-91	0.08944483615066556
92-93	0.16627633830662447
94-95	0.4392877009364337
96-97	0.362987659482499
98-99	0.06607828061833682
100-101	0.22360323269829682
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2822410.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.80899870312635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.42655258650825	92.38531451707713
2	3.154772574085476	6.045112029184279
3	0.21802968078020296	0.6266761620934055
4	0.08842728646127557	0.33888519095557335
5	0.07084213633920994	0.3393657074325032
6	0.026275014104688615	0.1510429675368443
7	0.0075177814178420555	0.05041897770786917
8	0.004940529533758975	0.03786777501541369
9	0.0	0.0
>10	0.0026424107693082464	0.02531667299697809
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.543071346827711E-5	0.0	0.0
2	3.543071346827711E-5	0.0	0.0
3	3.543071346827711E-5	0.0	0.0
4	1.7715356734138556E-4	0.0	0.0
5	2.480149942779398E-4	0.0	0.0
6	3.18876421214494E-4	0.0	0.0
7	4.960299885558796E-4	0.0	0.0
8	5.314607020241567E-4	0.0	0.0
9	5.668914154924338E-4	0.0	0.0
10-11	6.908989126314037E-4	0.0	0.0
12-13	7.97191053036235E-4	0.0	0.0
14-15	9.389139069093434E-4	0.0	3.543071346827711E-5
16-17	0.0010274906905800361	0.0	3.543071346827711E-5
18-19	0.0010629214040483133	0.0	5.314607020241567E-5
20-21	0.0010983521175165906	0.0	7.086142693655422E-5
22-23	0.0011337828309848676	0.0	8.857678367069278E-5
24-25	0.0012046442579214218	0.0	1.0629214040483133E-4
26-27	0.0012932210415921145	5.314607020241567E-5	1.0629214040483133E-4
28-29	0.001399513181996946	7.086142693655422E-5	1.0629214040483133E-4
30-31	0.0016475281762748856	7.086142693655422E-5	1.0629214040483133E-4
32-33	0.0020904120946283493	7.086142693655422E-5	1.0629214040483133E-4
34-35	0.002710449580323199	7.086142693655422E-5	1.0629214040483133E-4
36-37	0.0034722099198911567	8.857678367069278E-5	1.0629214040483133E-4
38-39	0.004960299885558796	1.0629214040483133E-4	1.0629214040483133E-4
40-41	0.006696404845504374	1.0629214040483133E-4	1.59438210607247E-4
42-43	0.008414794448715814	1.0629214040483133E-4	1.7715356734138556E-4
44-45	0.011833858298404554	1.0629214040483133E-4	1.7715356734138556E-4
46-47	0.01558951392604193	1.0629214040483133E-4	2.3029963754380122E-4
48-49	0.02074468273567625	1.0629214040483133E-4	2.834457077462169E-4
50-51	0.027299364727307512	1.0629214040483133E-4	2.834457077462169E-4
52-53	0.035430713468277114	1.0629214040483133E-4	3.3659177794863256E-4
54-55	0.045599328233672645	1.0629214040483133E-4	3.897378481510482E-4
56-57	0.05649427262516785	1.0629214040483133E-4	3.897378481510482E-4
58-59	0.06994022838637902	1.0629214040483133E-4	4.2516856161932534E-4
60-61	0.08632693336545719	1.0629214040483133E-4	4.2516856161932534E-4
62-63	0.10645157861543858	1.7715356734138556E-4	4.2516856161932534E-4
64-65	0.12864892060331418	1.7715356734138556E-4	4.2516856161932534E-4
66-67	0.15740094458282106	1.7715356734138556E-4	4.2516856161932534E-4
68-69	0.18960746312548493	1.7715356734138556E-4	4.428839183534639E-4
70-71	0.2265794126296321	1.7715356734138556E-4	4.6059927508760245E-4
72-73	0.2644371299704862	1.7715356734138556E-4	4.6059927508760245E-4
74-75	0.30667054042467257	1.7715356734138556E-4	4.6059927508760245E-4
76-77	0.354041404331759	2.1258428080966267E-4	4.6059927508760245E-4
78-79	0.4087109952133106	2.1258428080966267E-4	4.6059927508760245E-4
80-81	0.47190167268398286	2.1258428080966267E-4	4.6059927508760245E-4
82-83	0.5383165450802683	2.1258428080966267E-4	4.6059927508760245E-4
84-85	0.6100814552102636	2.480149942779398E-4	4.6059927508760245E-4
86-87	0.693627077568461	2.834457077462169E-4	4.78314631821741E-4
88-89	0.7843474194039846	2.834457077462169E-4	4.960299885558796E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACG	630	0.0	15.459276	3
GATACGG	805	8.0035534E-11	11.920681	4
ATGATAC	1110	0.0	11.698912	2
GTCCTAG	515	6.890578E-5	10.89693	1
ATACGGC	860	4.4128683E-9	10.302443	5
GTTCGTA	475	0.004710893	9.4516535	1
GTAGGCT	570	0.0021015757	8.860925	1
GTAACAG	790	6.0891565E-5	8.524434	1
GTACAAC	835	1.1620947E-4	8.065034	1
GGTCGTA	645	0.0064136228	7.830585	1
GTAGTCG	865	1.7504253E-4	7.7853208	1
GTCCGAG	1035	2.1696185E-5	7.591002	1
GGTTGCA	1595	9.222276E-9	7.3887334	1
GTATGAA	1140	9.777621E-6	7.3841043	1
GGTAAAT	1220	3.2131557E-6	7.359894	1
GATCCAT	1150	1.1058428E-5	7.319894	1
GGTCAAT	1160	1.24918915E-5	7.256792	1
GTATAAA	1490	1.6354716E-7	7.156138	1
GTATCGA	870	0.00158425	7.0955296	1
GGTTGAT	1555	3.4431105E-7	6.8570065	1
>>END_MODULE