FastQCFastQC Report
Thu 11 Sep 2014
LONLib43_TACAGC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib43_TACAGC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822410
Sequences flagged as poor quality0
Sequence length101
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG6300.015.4592763
GATACGG8058.0035534E-1111.9206814
ATGATAC11100.011.6989122
GTCCTAG5156.890578E-510.896931
ATACGGC8604.4128683E-910.3024435
GTTCGTA4750.0047108939.45165351
GTAGGCT5700.00210157578.8609251
GTAACAG7906.0891565E-58.5244341
GTACAAC8351.1620947E-48.0650341
GGTCGTA6450.00641362287.8305851
GTAGTCG8651.7504253E-47.78532081
GTCCGAG10352.1696185E-57.5910021
GGTTGCA15959.222276E-97.38873341
GTATGAA11409.777621E-67.38410431
GGTAAAT12203.2131557E-67.3598941
GATCCAT11501.1058428E-57.3198941
GGTCAAT11601.24918915E-57.2567921
GTATAAA14901.6354716E-77.1561381
GTATCGA8700.001584257.09552961
GGTTGAT15553.4431105E-76.85700651
Produced by FastQC (version 0.11.2)