##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	LONLib43_TACAGC_L007_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2822410
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18445087708731	33.0	33.0	33.0	27.0	33.0
2	31.552067913591575	33.0	33.0	33.0	27.0	33.0
3	31.747185561275646	33.0	33.0	33.0	27.0	33.0
4	35.595625015500936	37.0	37.0	37.0	33.0	37.0
5	35.138782458962375	37.0	37.0	37.0	33.0	37.0
6	34.95743814683196	37.0	37.0	37.0	33.0	37.0
7	34.72822516927023	37.0	33.0	37.0	27.0	37.0
8	34.79561899227965	37.0	33.0	37.0	27.0	37.0
9	34.68545250335706	37.0	33.0	37.0	27.0	37.0
10-11	34.51091549420531	37.0	33.0	37.0	27.0	37.0
12-13	34.520224205554825	37.0	33.0	37.0	27.0	37.0
14-15	35.430568556659026	37.0	33.0	40.0	27.0	40.0
16-17	35.32087931944686	37.0	33.0	40.0	27.0	40.0
18-19	35.52150874607162	37.0	33.0	40.0	27.0	40.0
20-21	35.14759460886264	37.0	33.0	40.0	27.0	40.0
22-23	35.157440804135476	37.0	33.0	40.0	27.0	40.0
24-25	34.96391948724671	37.0	33.0	40.0	27.0	40.0
26-27	34.52045255650313	37.0	33.0	40.0	27.0	40.0
28-29	34.10117629968715	37.0	33.0	40.0	22.0	40.0
30-31	33.95201884205342	37.0	33.0	37.0	22.0	40.0
32-33	33.881624214766816	37.0	33.0	37.0	22.0	40.0
34-35	33.67724869880705	37.0	33.0	37.0	22.0	40.0
36-37	33.28344003883207	37.0	33.0	37.0	22.0	40.0
38-39	33.10646557374726	37.0	33.0	37.0	22.0	40.0
40-41	32.73412686321265	37.0	33.0	37.0	22.0	40.0
42-43	32.273409603849196	37.0	33.0	37.0	22.0	40.0
44-45	32.03880283162262	37.0	27.0	37.0	18.5	40.0
46-47	31.834939112318903	35.0	27.0	37.0	18.5	40.0
48-49	31.817556981444937	33.0	27.0	37.0	22.0	40.0
50-51	32.1207776332992	33.0	27.0	37.0	22.0	40.0
52-53	31.962895893934615	33.0	27.0	37.0	22.0	40.0
54-55	31.577678473361416	33.0	27.0	37.0	18.5	40.0
56-57	31.587985622216472	33.0	27.0	37.0	18.5	40.0
58-59	31.28759553006119	33.0	27.0	37.0	15.0	40.0
60-61	31.1811046233538	33.0	27.0	37.0	15.0	40.0
62-63	31.145304013236917	33.0	27.0	37.0	15.0	40.0
64-65	30.80333243575526	33.0	27.0	37.0	15.0	40.0
66-67	30.456304364000978	33.0	27.0	37.0	15.0	37.0
68-69	30.25786614985066	33.0	27.0	37.0	15.0	37.0
70-71	30.09077791674491	33.0	27.0	37.0	15.0	37.0
72-73	29.889034017028003	33.0	27.0	37.0	15.0	37.0
74-75	29.532572517812792	33.0	27.0	37.0	15.0	37.0
76-77	28.45186170683919	33.0	24.5	33.0	15.0	37.0
78-79	29.432857557902643	33.0	27.0	37.0	15.0	37.0
80-81	29.167107719998157	33.0	27.0	37.0	15.0	37.0
82-83	29.147478218968892	33.0	27.0	37.0	15.0	37.0
84-85	29.043824072335347	33.0	27.0	37.0	15.0	37.0
86-87	28.8328816507878	33.0	27.0	33.0	15.0	37.0
88-89	28.71006037393575	33.0	27.0	33.0	15.0	37.0
90-91	28.628388327705757	33.0	27.0	33.0	15.0	37.0
92-93	28.405359426872778	33.0	27.0	33.0	15.0	37.0
94-95	28.287622280249856	33.0	27.0	33.0	15.0	37.0
96-97	28.154358863524436	33.0	22.0	33.0	15.0	37.0
98-99	27.86754723799873	33.0	22.0	33.0	15.0	37.0
100-101	27.035512912723526	33.0	22.0	33.0	10.5	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	1.201339013502384
2306	2	0.6463748321327394
2306	3	0.4455639902723618
2306	4	0.33305334084056426
2306	5	0.6436654034231211
2306	6	0.7213012223423902
2306	7	0.8421424137289364
2306	8	0.6884211873072275
2306	9	1.0522307305203995
2306	10-11	1.1496728109739038
2306	12-13	1.3750847225027414
2306	14-15	1.4839513077754347
2306	16-17	1.5005042911057558
2306	18-19	1.34443336414067
2306	20-21	1.5673008263648072
2306	22-23	1.32570606629708
2306	24-25	1.2433464246717136
2306	26-27	1.3191446858003033
2306	28-29	1.7023172203844652
2306	30-31	1.5550260849423978
2306	32-33	1.2721065654643198
2306	34-35	1.1674472681738877
2306	36-37	1.29118243440778
2306	38-39	1.018564803642633
2306	40-41	1.10179526882726
2306	42-43	1.2128015928527311
2306	44-45	0.9893972492261902
2306	46-47	0.8271008513210774
2306	48-49	0.8958428495078117
2306	50-51	1.0760192547254057
2306	52-53	1.2265307258844658
2306	54-55	1.5010680107523946
2306	56-57	1.0375575798950898
2306	58-59	0.982347106602159
2306	60-61	0.761690708110546
2306	62-63	0.44936033578408896
2306	64-65	0.6509873733820442
2306	66-67	0.7058033615230812
2306	68-69	0.5500722885743414
2306	70-71	0.5453578865393816
2306	72-73	0.45452902077904866
2306	74-75	0.5481484368669669
2306	76-77	0.5615864301337687
2306	78-79	0.6379406185321272
2306	80-81	1.0245494429114714
2306	82-83	0.9840577680083911
2306	84-85	0.6682160899878831
2306	86-87	0.5022566477688386
2306	88-89	0.31142507453197155
2306	90-91	0.03182835422713737
2306	92-93	-0.21091668990802148
2306	94-95	-0.09609517188164318
2306	96-97	-0.014383502662930425
2306	98-99	0.09582957966602024
2306	100-101	0.015093312018279903
2307	1	0.7897620472652989
2307	2	0.550076026684394
2307	3	0.36855012285210975
2307	4	0.3179544607920164
2307	5	0.6061033382617396
2307	6	0.5842378532578536
2307	7	0.861842482955403
2307	8	0.7644410006170403
2307	9	1.04040351219151
2307	10-11	1.2356990285220277
2307	12-13	1.3960159607081692
2307	14-15	1.3246550785631257
2307	16-17	1.3912161351533854
2307	18-19	1.222076376851156
2307	20-21	1.482748279052153
2307	22-23	1.1920687687212705
2307	24-25	1.1654882978323897
2307	26-27	1.3883290210207662
2307	28-29	1.4853769542893147
2307	30-31	1.6412942474064778
2307	32-33	1.3216774309215822
2307	34-35	1.3386890392334294
2307	36-37	1.3269359249705346
2307	38-39	1.043925220645157
2307	40-41	1.118870877403758
2307	42-43	1.1245714322410016
2307	44-45	0.9593794400067388
2307	46-47	0.9318798924554983
2307	48-49	0.9725647013631651
2307	50-51	0.9482304618263377
2307	52-53	1.2134826308766762
2307	54-55	1.4287382778274598
2307	56-57	1.1681003469938709
2307	58-59	1.2169060328649088
2307	60-61	0.9472157134052637
2307	62-63	0.8496653874194458
2307	64-65	0.9570454667144972
2307	66-67	1.0300853250258797
2307	68-69	0.8271812143629376
2307	70-71	0.7641581381570361
2307	72-73	0.6087588968984505
2307	74-75	0.8585671147363101
2307	76-77	0.8475116845173183
2307	78-79	0.8621963708088565
2307	80-81	1.4270129694150953
2307	82-83	1.1529037723137066
2307	84-85	1.0156359532419081
2307	86-87	0.9904240556960566
2307	88-89	0.8560978419784604
2307	90-91	0.5234284718172972
2307	92-93	0.5972810512229323
2307	94-95	0.6336415842104408
2307	96-97	0.5648293513324489
2307	98-99	0.57153253174803
2307	100-101	0.49935022755250813
1101	1	1.1031526979983752
1101	2	0.5801991625081833
1101	3	0.3998194787656075
1101	4	0.4100200976944848
1101	5	0.572514500604612
1101	6	0.5667863477531156
1101	7	0.7144330311016489
1101	8	0.659090073967711
1101	9	0.9490090401716955
1101	10-11	1.0120266098752566
1101	12-13	1.197827234753703
1101	14-15	1.3077281353285883
1101	16-17	1.3498921656508855
1101	18-19	1.0965839831531525
1101	20-21	1.3504250514166785
1101	22-23	1.0277306754331121
1101	24-25	1.1899612115964615
1101	26-27	1.2333328807308916
1101	28-29	1.4408381368887362
1101	30-31	1.3661568059989122
1101	32-33	0.9717652161873929
1101	34-35	1.066243892774196
1101	36-37	1.2327210541013969
1101	38-39	0.9002841131741022
1101	40-41	1.0114157255657616
1101	42-43	0.9706732494600523
1101	44-45	0.8473906649263405
1101	46-47	0.7498913709383785
1101	48-49	0.7485226973877808
1101	50-51	0.8557362433542473
1101	52-53	1.1075282599254237
1101	54-55	1.3808040459191169
1101	56-57	1.1108980272995232
1101	58-59	1.1451729947573561
1101	60-61	0.7290635850837859
1101	62-63	0.5978325890147467
1101	64-65	0.7531650359555968
1101	66-67	0.8457620898198073
1101	68-69	0.6511669599584948
1101	70-71	0.8291361520430769
1101	72-73	0.7516629078494788
1101	74-75	0.7860181888204991
1101	76-77	0.8034707507967376
1101	78-79	0.9163854876344821
1101	80-81	1.4953072176199385
1101	82-83	1.1652194806831666
1101	84-85	0.9735642443026826
1101	86-87	0.8525516294475821
1101	88-89	0.7944367105791166
1101	90-91	0.6080713678687459
1101	92-93	0.7517113539116274
1101	94-95	0.8062092447708302
1101	96-97	0.6882503560268951
1101	98-99	0.7889133402850987
1101	100-101	0.7154959003292518
2201	1	1.5529484712910069
2201	2	0.7711016668121431
2201	3	0.3392129307368634
2201	4	0.21476344735034303
2201	5	0.37593187175465914
2201	6	0.5092657635811548
2201	7	0.5374692948136826
2201	8	0.590954753302924
2201	9	0.63604719424319
2201	10-11	0.9196676173905516
2201	12-13	1.1493360550668967
2201	14-15	1.1117795063266982
2201	16-17	0.9847038213312089
2201	18-19	0.841869946165275
2201	20-21	1.1820590068942707
2201	22-23	0.9590979466348912
2201	24-25	0.8934899442938615
2201	26-27	1.094700059124861
2201	28-29	1.5086442635700337
2201	30-31	1.5178475165787546
2201	32-33	1.054985272726121
2201	34-35	0.9566462803123841
2201	36-37	1.0562940519515323
2201	38-39	0.5822129367814455
2201	40-41	1.1739560728473606
2201	42-43	1.0954734976010094
2201	44-45	0.9404868147006233
2201	46-47	0.8288005960917317
2201	48-49	0.9763650811594253
2201	50-51	0.9493423407437369
2201	52-53	1.0362148454068105
2201	54-55	1.4954413829899202
2201	56-57	1.1974595819992224
2201	58-59	1.2074850035505271
2201	60-61	0.8338097346156204
2201	62-63	0.683463145849597
2201	64-65	0.8362832646028551
2201	66-67	0.785082286190697
2201	68-69	0.7469199678308414
2201	70-71	0.8803127621190505
2201	72-73	0.6830862026402897
2201	74-75	0.8609847245673272
2201	76-77	0.7766550559955228
2201	78-79	0.7202530027174419
2201	80-81	1.380152607373887
2201	82-83	1.1729103802155372
2201	84-85	1.065427536213317
2201	86-87	1.0655241300251532
2201	88-89	1.0356450739598486
2201	90-91	0.790051652883605
2201	92-93	0.8900576314139563
2201	94-95	0.6500151266063661
2201	96-97	0.5108586219385742
2201	98-99	0.6915367302195463
2201	100-101	0.7591136795103886
2304	1	-2.1289758473336526
2304	2	-0.5986224388322618
2304	3	-0.24681333140475203
2304	4	0.021153251274391494
2304	5	0.3676632000112008
2304	6	0.6269246827924064
2304	7	0.6608507938427266
2304	8	0.7177754110039984
2304	9	0.9558066662345936
2304	10-11	0.9250900169298504
2304	12-13	1.1076429089769846
2304	14-15	1.214713965830292
2304	16-17	1.2393719896756465
2304	18-19	0.8445300787862067
2304	20-21	1.0741797942933857
2304	22-23	0.926010675380688
2304	24-25	1.0847710229905587
2304	26-27	1.1087470708675582
2304	28-29	1.497868834467802
2304	30-31	1.4495593190380873
2304	32-33	0.8400331800732772
2304	34-35	0.9420336364548518
2304	36-37	1.022271163787586
2304	38-39	0.8574147571775583
2304	40-41	0.8727195664897067
2304	42-43	0.9585968353331573
2304	44-45	1.1264843554819457
2304	46-47	1.0396234804456412
2304	48-49	0.912710506027846
2304	50-51	0.8398058097076877
2304	52-53	1.14534894019571
2304	54-55	1.5481504326553157
2304	56-57	1.2389545430552182
2304	58-59	0.917248983286779
2304	60-61	0.5051354819454517
2304	62-63	0.3670771300720368
2304	64-65	0.3517401329031813
2304	66-67	0.5990350687888757
2304	68-69	0.681720011785437
2304	70-71	0.5789588082720023
2304	72-73	0.37041025519432935
2304	74-75	0.6414065009761636
2304	76-77	0.7089907015571129
2304	78-79	0.6764102564409029
2304	80-81	1.3736982447011137
2304	82-83	1.101731065935752
2304	84-85	0.669355371692415
2304	86-87	0.5175918228854215
2304	88-89	0.5182213556436004
2304	90-91	0.25883954369112416
2304	92-93	0.29942076010522456
2304	94-95	0.31743458469179586
2304	96-97	0.2791765802593673
2304	98-99	0.673859275267187
2304	100-101	0.5144271919996086
1102	1	1.5051031658200706
1102	2	0.1783284492408015
1102	3	0.3392719120740715
1102	4	0.2587899897348933
1102	5	0.34635304727709837
1102	6	0.1698684221792348
1102	7	-5.152659439255942
1102	8	-1.2750213730750275
1102	9	0.2099947371631785
1102	10-11	0.7478670101989096
1102	12-13	0.9276332322483967
1102	14-15	0.8722859521829776
1102	16-17	0.6796454355278669
1102	18-19	0.8247545044838773
1102	20-21	1.3028646028412254
1102	22-23	0.7794281331327326
1102	24-25	1.163663864872163
1102	26-27	1.1750161713930538
1102	28-29	1.0441349810697247
1102	30-31	1.3594836498405698
1102	32-33	1.0488462006943422
1102	34-35	1.3062206271460042
1102	36-37	1.3936915827922505
1102	38-39	1.0654256258318426
1102	40-41	1.2899936343547438
1102	42-43	1.4493232838471037
1102	44-45	1.1688710277420462
1102	46-47	1.1271645549600322
1102	48-49	1.1374955459504257
1102	50-51	0.9780310106907955
1102	52-53	1.1331344901241849
1102	54-55	1.382528387176361
1102	56-57	1.238075774691584
1102	58-59	1.2103620674156055
1102	60-61	1.108835028245732
1102	62-63	0.8450108908055043
1102	64-65	0.6153807393465627
1102	66-67	0.782677163346932
1102	68-69	0.9594123083861277
1102	70-71	0.8940768504994097
1102	72-73	0.8128800244791918
1102	74-75	0.8908783118778949
1102	76-77	0.8412004498872854
1102	78-79	0.9480513314651482
1102	80-81	1.4659638443332597
1102	82-83	1.3651590703421022
1102	84-85	0.9343140709336026
1102	86-87	0.742963943168423
1102	88-89	0.71745125231363
1102	90-91	0.4372657285825241
1102	92-93	0.6281465475712196
1102	94-95	0.591571756999862
1102	96-97	0.31095181184920406
1102	98-99	0.4120241430783089
1102	100-101	0.6634733487283562
2202	1	1.5288754716239872
2202	2	0.9690250211648106
2202	3	0.4423276527518709
2202	4	-0.2579222272719619
2202	5	0.04062289492006954
2202	6	0.43689952579645563
2202	7	0.7264551093669738
2202	8	-3.9501995696341154
2202	9	-0.40234362887090214
2202	10-11	0.720034045170415
2202	12-13	0.2382477237087528
2202	14-15	0.3257719779923818
2202	16-17	0.3245870586979436
2202	18-19	0.7626414213787669
2202	20-21	1.3162384796569455
2202	22-23	-2.906761920206435
2202	24-25	-3.231414717380133
2202	26-27	-1.5862115210915775
2202	28-29	0.7066805955894253
2202	30-31	1.2557714810967084
2202	32-33	1.1929394990524074
2202	34-35	-2.077643291062934
2202	36-37	-0.2554051504533916
2202	38-39	0.41977834890870014
2202	40-41	1.2460569596819013
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1209	86-87	-1.8143117579456316
1209	88-89	-1.0535541139123907
1209	90-91	-5.653028435661682
1209	92-93	-3.056970096731174
1209	94-95	-0.45279712633062985
1209	96-97	0.6557597391248997
1209	98-99	1.7483902562791513
1209	100-101	1.2615104934304249
1212	1	-28.566033535517224
1212	2	-16.1799086806519
1212	3	-5.943854506392071
1212	4	-1.5787836322113193
1212	5	-0.311040032836182
1212	6	-2.165952909093022
1212	7	-0.41009385368391804
1212	8	-1.4325037675764847
1212	9	-0.1157653762987465
1212	10-11	0.5851743216091805
1212	12-13	0.17075842528271323
1212	14-15	-0.48437117737167057
1212	16-17	-0.2827936386142156
1212	18-19	-1.3177318121677004
1212	20-21	-0.7329978130495931
1212	22-23	-0.6691910280111699
1212	24-25	0.08792074995508159
1212	26-27	-0.7803867536627678
1212	28-29	-0.07765431073056561
1212	30-31	-0.9012123723846983
1212	32-33	0.05126969379735158
1212	34-35	-1.3681921259498395
1212	36-37	-1.642367132678622
1212	38-39	-0.6398123076982856
1212	40-41	-0.5498283092492642
1212	42-43	-0.18855623507678843
1212	44-45	0.7810079311411506
1212	46-47	-1.9890214594439755
1212	48-49	-1.9649124275771754
1212	50-51	-0.5230064767667706
1212	52-53	-1.4576705840086746
1212	54-55	-0.4619709413330959
1212	56-57	-1.755336472865622
1212	58-59	-0.6333760720177928
1212	60-61	-0.09117974224125902
1212	62-63	-4.81057323681765
1212	64-65	-4.528716501549518
1212	66-67	-1.7437067871072074
1212	68-69	0.05802816585803683
1212	70-71	-1.234380864803967
1212	72-73	-1.7166051520214012
1212	74-75	-0.49997249506320074
1212	76-77	1.026271386639639
1212	78-79	-1.1893964052122215
1212	80-81	0.342667252186164
1212	82-83	0.45414232302998414
1212	84-85	-3.5383470829545125
1212	86-87	-2.118652247743867
1212	88-89	-0.8848136261189232
1212	90-91	-0.006608533661420779
1212	92-93	-2.8857971570897227
1212	94-95	-2.149408066015514
1212	96-97	0.391513774138172
1212	98-99	-0.5545184950343867
1212	100-101	0.13795747650517143
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	11.0
11	29.0
12	117.0
13	452.0
14	1467.0
15	3200.0
16	5851.0
17	9509.0
18	14410.0
19	20276.0
20	27254.0
21	36188.0
22	45655.0
23	56552.0
24	68962.0
25	82549.0
26	97922.0
27	115145.0
28	133533.0
29	153182.0
30	175237.0
31	199804.0
32	223301.0
33	247475.0
34	267898.0
35	280710.0
36	271577.0
37	215520.0
38	68463.0
39	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	57.16918519321864	23.91931915233667	8.564571862377788	10.346923792066908
2	28.536735907231392	22.53287527273526	27.916717156792924	21.013671663240423
3	21.919961734693878	23.37769274376417	32.049284297052154	22.653061224489797
4	26.620521689096087	20.013966792197728	28.21309739304261	25.152414125663576
5	22.737908383261114	25.806916783883278	26.422489999681126	25.032684833174486
6	22.223950453690286	24.82385620799246	26.28576287640704	26.666430461910213
7	26.974217069809136	25.57810523630514	27.128446965536547	20.319230728349176
8	21.33386006994023	23.258775301958256	28.534018799536565	26.873345828564947
9	25.60396965713699	24.891954039278488	24.242757076399247	25.26131922718528
10-11	25.548024560570575	25.38181908369089	24.124684223766213	24.94547213197232
12-13	25.164012893154425	24.733264970225132	24.847292221052076	25.255429915568367
14-15	24.449849596621327	25.33691065436985	25.241017428367957	24.97222232064087
16-17	24.96723974307668	25.506922569580446	24.82817362608434	24.697664061258536
18-19	25.39759841265675	25.574539371801958	24.244103682400823	24.783758533140475
20-21	24.576239659099727	25.59237516603724	24.93346995421287	24.89791522065016
22-23	24.90104642859231	24.92301347483418	24.981421016333737	25.194519080239775
24-25	25.186879298188426	24.62891642249	24.992311535177382	25.19189274414419
26-27	24.879004113505836	24.99174464376189	25.071499179778982	25.05775206295329
28-29	25.36971949504147	24.920440332906985	24.84849826920965	24.861341902841897
30-31	25.363519120184524	24.74295017378765	24.643496161082197	25.25003454494563
32-33	24.78925811629069	25.031214458565554	25.062446632487838	25.117080792655923
34-35	25.434203393553734	24.850624112017744	24.713223805187763	25.001948689240756
36-37	25.444655454026876	24.724171895649462	24.658412491452342	25.17276015887132
38-39	24.85583242689758	24.908110444620025	25.164965401908297	25.071091726574096
40-41	25.465301757889314	24.881057072055253	24.719950878023443	24.933690292031994
42-43	25.234799338154275	24.675631818197925	24.930024340900154	25.15954450274765
44-45	24.84543351249464	24.77056841493617	25.252798140596155	25.13119993197303
46-47	25.39460175514013	24.8296547307735	24.8596890815655	24.916054432520877
48-49	25.385663316102196	24.616728257056913	24.79994047640137	25.197667950439516
50-51	24.97914016005476	24.97958304413003	25.005234889769696	25.036041906045508
52-53	25.22560522787634	24.958120867004347	24.96731514366456	24.84895876145476
54-55	25.060972725567197	24.649952690444213	25.00798531446195	25.281089269526642
56-57	24.855104146441363	24.93987326971781	25.148740137728126	25.056282446112704
58-59	25.22184730011756	24.76644992898546	25.0479724949421	24.963730275954884
60-61	25.354781996813315	24.549110728689786	24.830984064085214	25.265123210411687
62-63	24.7921344313676	24.910350389051498	25.203064650824462	25.094450528756436
64-65	25.30091965980099	24.850142505459992	24.856785910171247	24.99215192456777
66-67	25.327065384731224	24.69499113310267	24.834611548402986	25.143331933763125
68-69	24.939765055370085	24.86168993743361	25.09417910754489	25.104365899651416
70-71	25.4668367631659	24.843395653823034	24.65333921265824	25.036428370352827
72-73	25.231804252296598	24.753823175179505	24.832633076552824	25.181739495971073
74-75	24.847177522906755	24.789134136228448	25.211760883932545	25.15192745693225
76-77	25.309739380680924	24.983880955042327	24.861493313745633	24.84488635053112
78-79	25.399828302093365	24.700263853551537	24.68853624168659	25.211371602668507
80-81	24.918564724439555	24.843261369503065	25.055958763698392	25.182215142358988
82-83	25.29609060482348	24.917920913531553	24.77438438027721	25.011604101367762
84-85	25.194178868137655	24.57818514110455	24.83326359039482	25.39437240036297
86-87	24.840051158117664	24.879789230539462	25.098304337650664	25.18185527369221
88-89	25.299193027891377	24.786865217462942	24.758146655297352	25.15579509934833
90-91	25.347612230762316	24.573156048482055	24.601861267675638	25.47737045307999
92-93	24.88109943425229	24.795277002352144	25.02808186510358	25.295541698291984
94-95	25.308634756956437	24.811509465720537	24.632365057006936	25.247490720316083
96-97	25.292502047221998	24.634644842860872	24.585444189592423	25.487408920324704
98-99	24.878754628921747	24.7428435864286	24.910185497046225	25.46821628760343
100-101	25.327210335225818	24.593148639387564	24.52955984292203	25.55008118246459
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	6.0
2	7.0
3	10.5
4	17.0
5	24.5
6	29.5
7	41.0
8	74.0
9	117.0
10	147.5
11	200.5
12	317.5
13	448.0
14	645.5
15	979.0
16	1382.0
17	1947.5
18	2776.5
19	3852.0
20	5216.5
21	7091.5
22	9204.0
23	11669.5
24	14788.5
25	18197.0
26	21808.0
27	25831.0
28	30207.0
29	34420.5
30	38541.5
31	42770.0
32	46805.5
33	50753.0
34	54692.0
35	58234.0
36	61410.0
37	64445.5
38	66661.0
39	67469.5
40	67591.5
41	67218.5
42	65879.0
43	64073.5
44	62078.0
45	60400.5
46	58821.5
47	57418.5
48	55698.0
49	53245.5
50	51420.5
51	49963.5
52	48565.0
53	48383.0
54	49183.5
55	50211.0
56	51990.5
57	54094.5
58	56230.5
59	58956.5
60	61702.5
61	63872.0
62	65885.0
63	67692.5
64	68669.0
65	68382.5
66	67507.0
67	66008.0
68	63236.5
69	59522.0
70	54782.0
71	49235.5
72	43167.0
73	36680.0
74	30147.5
75	23784.5
76	18159.0
77	13203.0
78	9076.5
79	6108.5
80	3921.5
81	2382.0
82	1490.0
83	972.5
84	669.0
85	475.0
86	319.0
87	220.5
88	149.0
89	107.0
90	79.5
91	56.5
92	35.5
93	20.0
94	14.0
95	8.0
96	2.0
97	2.0
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6843796613532407
2	0.0026927342235890605
3	3.543071346827711E-4
4	3.543071346827711E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.18073206940168154
14-15	0.0
16-17	4.074532048851868E-4
18-19	0.11011865745940526
20-21	3.543071346827711E-5
22-23	1.7715356734138556E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0017006742464773011
42-43	0.0
44-45	0.0
46-47	0.02271108733316563
48-49	0.0
50-51	3.543071346827711E-5
52-53	7.086142693655422E-5
54-55	0.0014703746089335002
56-57	0.0013463671117945302
58-59	0.09087978004613079
60-61	0.001523520679135916
62-63	0.0011692135444531446
64-65	0.0021967042350331807
66-67	0.0040745320488518675
68-69	0.00453513132393947
70-71	0.0032064795688790783
72-73	0.03769827913024685
74-75	0.04409352291127087
76-77	0.15288352861561572
78-79	3.897378481510482E-4
80-81	0.11148273992793392
82-83	0.004676854177812578
84-85	0.004995730599027073
86-87	0.006359813067555741
88-89	0.006855843056111621
90-91	0.02209104984747078
92-93	0.010770936894356242
94-95	0.013640824685286686
96-97	0.010381199046205193
98-99	0.012170450076353187
100-101	0.013180225410199083
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2822410.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.84477979019591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.00109242340686	87.27510726089062
2	5.249169261909999	9.74715928406998
3	0.4003761504295808	1.1151850655964235
4	0.12839233389034374	0.47682231867193087
5	0.08391177851339017	0.3895385298939702
6	0.04066005367544516	0.22650442378525515
7	0.04847343827089681	0.31503539913545486
8	0.016989740370839026	0.12619269627385207
9	0.009074026644928658	0.07582284050928943
>10	0.021811956209830268	0.2283287051381796
>50	0.0	0.0
>100	0.0	0.0
>500	4.883667788104997E-5	0.02430347603503237
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.543071346827711E-5	0.0	0.0
2	3.543071346827711E-5	0.0	0.0
3	3.543071346827711E-5	0.0	3.543071346827711E-5
4	1.7715356734138556E-4	0.0	3.543071346827711E-5
5	2.480149942779398E-4	0.0	3.543071346827711E-5
6	3.18876421214494E-4	0.0	7.086142693655422E-5
7	6.023221289607109E-4	0.0	7.086142693655422E-5
8	6.731835558972651E-4	0.0	7.086142693655422E-5
9	6.731835558972651E-4	0.0	7.086142693655422E-5
10-11	8.326217665045122E-4	0.0	7.086142693655422E-5
12-13	0.0010274906905800361	0.0	7.086142693655422E-5
14-15	0.001151498187719006	0.0	7.086142693655422E-5
16-17	0.001240074971389699	0.0	7.086142693655422E-5
18-19	0.0012932210415921145	0.0	7.086142693655422E-5
20-21	0.00134636711179453	1.7715356734138556E-5	7.086142693655422E-5
22-23	0.001434943895465223	3.543071346827711E-5	7.086142693655422E-5
24-25	0.001558951392604193	3.543071346827711E-5	1.0629214040483133E-4
26-27	0.001629812819540747	3.543071346827711E-5	1.0629214040483133E-4
28-29	0.0017715356734138556	3.543071346827711E-5	1.0629214040483133E-4
30-31	0.0021258428080966267	3.543071346827711E-5	1.0629214040483133E-4
32-33	0.0026750188668549215	3.543071346827711E-5	1.0629214040483133E-4
34-35	0.0034013484929546027	3.543071346827711E-5	1.0629214040483133E-4
36-37	0.004499700610471193	3.543071346827711E-5	1.240074971389699E-4
38-39	0.006218090213682633	3.543071346827711E-5	2.1258428080966267E-4
40-41	0.00809591802750132	1.4172285387310845E-4	2.480149942779398E-4
42-43	0.009973745841320006	1.4172285387310845E-4	2.480149942779398E-4
44-45	0.013428240404477026	1.4172285387310845E-4	2.480149942779398E-4
46-47	0.01730790352925337	1.4172285387310845E-4	2.480149942779398E-4
48-49	0.02302996375438012	1.7715356734138556E-4	2.480149942779398E-4
50-51	0.030860151430869363	1.948689240755241E-4	2.480149942779398E-4
52-53	0.03985955265181175	2.1258428080966267E-4	2.480149942779398E-4
54-55	0.05016989027108039	2.1258428080966267E-4	2.480149942779398E-4
56-57	0.06191517178581425	2.1258428080966267E-4	2.480149942779398E-4
58-59	0.07596344967598612	2.1258428080966267E-4	2.480149942779398E-4
60-61	0.09406854425827574	2.1258428080966267E-4	2.480149942779398E-4
62-63	0.11488408842088853	2.3029963754380122E-4	2.834457077462169E-4
64-65	0.13798491360220522	2.480149942779398E-4	2.834457077462169E-4
66-67	0.16730382899720453	2.480149942779398E-4	2.834457077462169E-4
68-69	0.20154761356429435	2.480149942779398E-4	2.834457077462169E-4
70-71	0.24084027480061365	2.480149942779398E-4	2.834457077462169E-4
72-73	0.2834988538164193	2.480149942779398E-4	2.834457077462169E-4
74-75	0.3302319648810768	2.480149942779398E-4	2.834457077462169E-4
76-77	0.3843169489904018	2.480149942779398E-4	2.834457077462169E-4
78-79	0.44591324435500157	2.480149942779398E-4	2.834457077462169E-4
80-81	0.5167392405780875	2.480149942779398E-4	2.834457077462169E-4
82-83	0.5921357988385811	2.6573035101207834E-4	2.834457077462169E-4
84-85	0.6745122076523256	2.834457077462169E-4	2.834457077462169E-4
86-87	0.7687047594077402	2.834457077462169E-4	3.18876421214494E-4
88-89	0.8708692216935172	2.834457077462169E-4	3.18876421214494E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTACT	630	2.6390026E-6	9.860062	1
GTCTAAG	530	4.6557141E-4	9.015732	1
GTAGTCT	630	2.8048354E-4	8.343129	1
GTAACCG	620	0.0022024857	7.706996	1
GTAACTT	1120	1.5596379E-7	7.6794705	1
GGTGCCT	1205	6.627852E-8	7.534308	1
CTCGTAT	695	2.075467E-9	7.513378	42-43
GTAACCT	645	0.0032443537	7.4082747	1
GTATCTT	1230	9.511132E-8	7.381171	1
GGTGTAA	715	0.0010884107	7.351288	1
GTCTAGT	780	3.3941836E-4	7.351288	1
GGTTAAG	875	1.6311054E-4	7.099244	1
GTGTCCA	1235	7.9603706E-7	6.964379	1
GCTCTAT	1180	2.894718E-6	6.884045	1
GTATGAG	765	0.0022259096	6.8708115	1
GTAGAAC	975	8.237431E-5	6.8612022	1
GGAACAT	1325	3.4895857E-7	6.8519554	1
GTTGCAA	1325	3.4895857E-7	6.8519554	1
GTAGTTA	770	0.0023839958	6.826196	1
GTTTAGT	1055	3.1459218E-5	6.7938447	1
>>END_MODULE