FastQCFastQC Report
Thu 11 Sep 2014
LONLib43_TACAGC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib43_TACAGC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822410
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACT6302.6390026E-69.8600621
GTCTAAG5304.6557141E-49.0157321
GTAGTCT6302.8048354E-48.3431291
GTAACCG6200.00220248577.7069961
GTAACTT11201.5596379E-77.67947051
GGTGCCT12056.627852E-87.5343081
CTCGTAT6952.075467E-97.51337842-43
GTAACCT6450.00324435377.40827471
GTATCTT12309.511132E-87.3811711
GGTGTAA7150.00108841077.3512881
GTCTAGT7803.3941836E-47.3512881
GGTTAAG8751.6311054E-47.0992441
GTGTCCA12357.9603706E-76.9643791
GCTCTAT11802.894718E-66.8840451
GTATGAG7650.00222590966.87081151
GTAGAAC9758.237431E-56.86120221
GGAACAT13253.4895857E-76.85195541
GTTGCAA13253.4895857E-76.85195541
GTAGTTA7700.00238399586.8261961
GTTTAGT10553.1459218E-56.79384471
Produced by FastQC (version 0.11.2)