##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	LONLib42_TAATCG_L007_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2140450
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.812003083463757	27.0	22.0	33.0	2.0	33.0
2	24.766550024527554	27.0	15.0	33.0	2.0	33.0
3	25.69622976476909	33.0	22.0	33.0	6.0	33.0
4	27.85419187554019	33.0	22.0	37.0	2.0	37.0
5	27.923973930715505	33.0	15.0	37.0	6.0	37.0
6	28.97528790674858	33.0	22.0	37.0	15.0	37.0
7	28.64897568268355	33.0	27.0	37.0	6.0	37.0
8	28.403524492513256	33.0	22.0	37.0	2.0	37.0
9	29.167141021747764	33.0	27.0	37.0	15.0	37.0
10-11	28.782033684505596	33.0	24.5	37.0	10.5	37.0
12-13	29.07440351327992	33.0	27.0	37.0	10.5	37.0
14-15	29.413450209068188	33.0	27.0	37.0	6.0	40.0
16-17	30.270394309607795	33.0	27.0	37.0	15.0	40.0
18-19	30.582677474362868	33.0	27.0	37.0	15.0	40.0
20-21	30.5854673082763	33.0	27.0	37.0	15.0	40.0
22-23	30.600334041907075	33.0	27.0	37.0	15.0	40.0
24-25	30.183637085659555	33.0	27.0	37.0	10.5	40.0
26-27	29.65896353570511	33.0	27.0	37.0	10.5	40.0
28-29	29.854993108925694	33.0	27.0	37.0	15.0	40.0
30-31	29.764316849260666	33.0	27.0	37.0	15.0	40.0
32-33	29.576485552103527	33.0	27.0	37.0	10.5	40.0
34-35	29.61802378004625	33.0	27.0	37.0	10.5	40.0
36-37	29.839786026302882	33.0	27.0	37.0	10.5	40.0
38-39	29.871628395898057	33.0	27.0	37.0	15.0	40.0
40-41	29.948421827185875	33.0	27.0	37.0	15.0	40.0
42-43	29.85469153682637	33.0	27.0	37.0	15.0	40.0
44-45	29.586792730500598	33.0	27.0	37.0	15.0	40.0
46-47	29.212641033427552	33.0	22.0	37.0	10.5	40.0
48-49	29.049529304585484	33.0	22.0	37.0	10.5	40.0
50-51	28.275427363404894	33.0	22.0	37.0	10.5	38.5
52-53	28.001362797542573	33.0	22.0	37.0	6.0	37.0
54-55	28.935830549650774	33.0	22.0	37.0	15.0	40.0
56-57	28.849719217921468	33.0	22.0	37.0	10.5	40.0
58-59	29.039184050082927	33.0	22.0	37.0	10.5	40.0
60-61	28.789722021070336	33.0	22.0	37.0	10.5	40.0
62-63	28.659395454226917	33.0	22.0	37.0	15.0	40.0
64-65	28.614200752178277	33.0	22.0	37.0	10.5	40.0
66-67	28.4922642435002	33.0	22.0	37.0	10.5	40.0
68-69	28.191909177976594	33.0	22.0	37.0	6.0	38.5
70-71	28.087669181714126	33.0	22.0	37.0	6.0	37.0
72-73	27.854119694456774	33.0	22.0	37.0	6.0	37.0
74-75	27.79232965030718	33.0	22.0	37.0	15.0	37.0
76-77	27.40813987712864	30.0	22.0	37.0	6.0	37.0
78-79	26.85867831530753	27.0	22.0	33.0	6.0	37.0
80-81	26.735336728258076	27.0	22.0	33.0	6.0	37.0
82-83	26.32612978579271	27.0	22.0	33.0	6.0	37.0
84-85	26.433072718353618	27.0	22.0	33.0	6.0	37.0
86-87	26.129986685042866	27.0	22.0	33.0	6.0	37.0
88-89	25.784778668037095	27.0	22.0	33.0	6.0	37.0
90-91	25.659766637856528	27.0	22.0	33.0	6.0	37.0
92-93	25.558108575299585	27.0	22.0	33.0	6.0	37.0
94-95	25.356232100726483	27.0	22.0	33.0	6.0	37.0
96-97	25.234478964703683	27.0	18.5	33.0	6.0	37.0
98-99	25.005796678268588	27.0	15.0	33.0	6.0	37.0
100-101	24.53710644957836	27.0	15.0	33.0	6.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	8.273545536166893
2306	2	8.54987151921172
2306	3	7.6119452585683085
2306	4	9.044044019310196
2306	5	8.96552561678126
2306	6	7.912140851516277
2306	7	8.271232281855273
2306	8	8.694786209673449
2306	9	7.581775072642415
2306	10-11	7.798543737139632
2306	12-13	7.507877010788057
2306	14-15	8.076768172942142
2306	16-17	6.877251949955927
2306	18-19	6.319635367129003
2306	20-21	6.114084375246993
2306	22-23	5.900548824050457
2306	24-25	5.925003146354783
2306	26-27	6.639168922301298
2306	28-29	5.631176185060067
2306	30-31	5.54731817438234
2306	32-33	5.559626975362523
2306	34-35	4.949940436936803
2306	36-37	4.985974450483159
2306	38-39	4.67347873646942
2306	40-41	3.901622098325273
2306	42-43	3.73707034512055
2306	44-45	3.453184884774007
2306	46-47	3.73683629318894
2306	48-49	3.4886350775438864
2306	50-51	3.357158068641219
2306	52-53	3.9032761306444215
2306	54-55	3.4990107187740627
2306	56-57	3.707840842559314
2306	58-59	3.324761731677672
2306	60-61	3.5340590410934283
2306	62-63	3.0364244992961282
2306	64-65	2.515884055212915
2306	66-67	2.4332305550537576
2306	68-69	2.4421486804854737
2306	70-71	2.61135511568769
2306	72-73	1.7720051728130564
2306	74-75	1.3612327736595873
2306	76-77	1.7905336542632035
2306	78-79	1.9886099748681794
2306	80-81	1.8916350619148332
2306	82-83	1.8274053897056923
2306	84-85	1.4602339179262493
2306	86-87	1.4295511547356519
2306	88-89	1.315926509864628
2306	90-91	1.2727871408649136
2306	92-93	1.181010651105133
2306	94-95	1.4654814186481602
2306	96-97	1.253006631504384
2306	98-99	1.1662683121793442
2306	100-101	0.8679607076786944
2307	1	8.264523413302552
2307	2	8.373179934397289
2307	3	7.518014631500929
2307	4	9.004367516887502
2307	5	8.949608686265467
2307	6	7.548076597088162
2307	7	8.162498454205231
2307	8	8.487941980129765
2307	9	7.464836464349624
2307	10-11	7.670768769256732
2307	12-13	7.222984185798431
2307	14-15	8.058121036168831
2307	16-17	6.790601535721223
2307	18-19	6.293262424393532
2307	20-21	6.218054250925999
2307	22-23	5.892906471966139
2307	24-25	6.069734930649261
2307	26-27	6.891196092860785
2307	28-29	5.973330003201422
2307	30-31	5.887331021221719
2307	32-33	5.866229441551059
2307	34-35	5.652321654250702
2307	36-37	5.396044551740527
2307	38-39	4.9082456219707
2307	40-41	4.39833098085256
2307	42-43	4.272969674454842
2307	44-45	4.343946287608407
2307	46-47	4.564185393404401
2307	48-49	4.330601250905406
2307	50-51	4.011379823296934
2307	52-53	4.7848105692780045
2307	54-55	4.279125429922491
2307	56-57	4.522220704379926
2307	58-59	4.216449219665371
2307	60-61	4.149772647615979
2307	62-63	3.756891942168153
2307	64-65	3.7036218987647125
2307	66-67	3.35679540124573
2307	68-69	3.6089876499059486
2307	70-71	3.214844499342867
2307	72-73	2.502240259857981
2307	74-75	2.264973024729315
2307	76-77	2.5086557902778495
2307	78-79	2.8904515237721533
2307	80-81	2.6221892968197977
2307	82-83	2.7108992242343533
2307	84-85	1.9917951629569508
2307	86-87	2.12413676829868
2307	88-89	2.234402186852602
2307	90-91	2.1096712259575234
2307	92-93	1.988584216962888
2307	94-95	2.1224252870641536
2307	96-97	1.8179853381210123
2307	98-99	1.5669794403746202
2307	100-101	1.3441257743912196
1101	1	8.140493784886871
1101	2	7.951093476173263
1101	3	7.315222145185697
1101	4	8.853019355279375
1101	5	8.631446170722821
1101	6	7.391246675196669
1101	7	7.114909263948103
1101	8	8.074885419517532
1101	9	7.442883544432775
1101	10-11	7.59604468108644
1101	12-13	7.350532713721449
1101	14-15	7.958490511391577
1101	16-17	6.986767255691213
1101	18-19	6.178013935558354
1101	20-21	5.8811172457512875
1101	22-23	5.369727408668336
1101	24-25	5.9460114241129105
1101	26-27	6.486837500163222
1101	28-29	5.920217939931913
1101	30-31	5.651007201282411
1101	32-33	5.690196043065011
1101	34-35	5.4733532610922815
1101	36-37	5.3300687697399916
1101	38-39	5.025818919721573
1101	40-41	4.4298155014133265
1101	42-43	4.294521615160164
1101	44-45	4.251381474976004
1101	46-47	4.626114552150909
1101	48-49	4.206488687375835
1101	50-51	3.8913649072771364
1101	52-53	4.858238163517225
1101	54-55	4.479824407819361
1101	56-57	4.830713260042728
1101	58-59	4.208863873850547
1101	60-61	4.3335425722685095
1101	62-63	3.892745217698284
1101	64-65	3.741111763696388
1101	66-67	3.410097557662777
1101	68-69	3.5728592597261866
1101	70-71	3.592236548150538
1101	72-73	3.015479315569088
1101	74-75	2.401308960859531
1101	76-77	2.8416755424603473
1101	78-79	3.277575225208391
1101	80-81	2.901573614541995
1101	82-83	2.8810339396240394
1101	84-85	2.38339901640715
1101	86-87	2.3632170808084076
1101	88-89	2.3883775890453265
1101	90-91	2.2839366588196803
1101	92-93	2.1535515259564164
1101	94-95	2.2592690529220043
1101	96-97	2.1523854892114755
1101	98-99	2.045638716906023
1101	100-101	1.5493250821834401
2201	1	7.049535754970222
2201	2	7.739458620258613
2201	3	6.345216610360254
2201	4	8.303016484222699
2201	5	3.9521121319752304
2201	6	5.676169481894021
2201	7	6.703500042114062
2201	8	7.849367324831757
2201	9	6.565541852234354
2201	10-11	4.184850061804042
2201	12-13	6.472434182392028
2201	14-15	7.097869067004655
2201	16-17	5.947235902680141
2201	18-19	2.134377209763805
2201	20-21	5.038387082906379
2201	22-23	5.262723102559843
2201	24-25	5.44432865133453
2201	26-27	6.241556909769134
2201	28-29	4.9759251598174465
2201	30-31	5.072641030287997
2201	32-33	5.3685421285758
2201	34-35	5.044285682612955
2201	36-37	4.986276292099088
2201	38-39	4.3370488732533445
2201	40-41	4.251722712197246
2201	42-43	4.05510570007101
2201	44-45	3.9746856871377965
2201	46-47	4.269928068323303
2201	48-49	4.085256234476052
2201	50-51	3.760803092712134
2201	52-53	4.5421028964815555
2201	54-55	4.156175954770905
2201	56-57	4.188404534961059
2201	58-59	3.8763167891167285
2201	60-61	3.875201199579383
2201	62-63	3.641465381641577
2201	64-65	3.5282111649202434
2201	66-67	3.333135035555042
2201	68-69	3.560310121174229
2201	70-71	3.297155823805575
2201	72-73	2.723352634541577
2201	74-75	2.248718445734781
2201	76-77	2.690458512257546
2201	78-79	2.9456656818398272
2201	80-81	2.5762704321484122
2201	82-83	2.8276804858619897
2201	84-85	2.3333496366149546
2201	86-87	2.34652942723368
2201	88-89	2.4554804974853006
2201	90-91	2.17883670911678
2201	92-93	2.0429556828176096
2201	94-95	2.189968797338395
2201	96-97	1.9711808081263058
2201	98-99	1.9168350612050453
2201	100-101	1.6509034723950649
2304	1	1.1603510753731143
2304	2	4.465207454835632
2304	3	5.647334801692093
2304	4	4.42224504861186
2304	5	6.444418693715203
2304	6	6.280862276152654
2304	7	7.060573994940963
2304	8	6.36713596976988
2304	9	5.809747852180685
2304	10-11	6.643994515707938
2304	12-13	6.533709786936136
2304	14-15	6.83957105413926
2304	16-17	5.600723359289326
2304	18-19	5.321495443785139
2304	20-21	4.953769934417711
2304	22-23	4.6246773784607775
2304	24-25	4.750692251107175
2304	26-27	5.664450732478482
2304	28-29	5.040397993447979
2304	30-31	4.92021413298427
2304	32-33	5.09607742668258
2304	34-35	4.672920326472859
2304	36-37	4.804197704413664
2304	38-39	4.4374372865949
2304	40-41	3.786484395089989
2304	42-43	3.301983330048298
2304	44-45	3.1518342794824754
2304	46-47	4.035781388467619
2304	48-49	3.70665156473412
2304	50-51	3.3929721516163056
2304	52-53	4.075297936207168
2304	54-55	3.8432625680797763
2304	56-57	4.505411142073861
2304	58-59	3.769329587424764
2304	60-61	3.8534434965664808
2304	62-63	3.5724856664658304
2304	64-65	3.2862929860293413
2304	66-67	3.08889259708279
2304	68-69	3.3064534309998876
2304	70-71	3.2130282017563303
2304	72-73	2.7763490400868207
2304	74-75	2.3257236318975316
2304	76-77	2.6012648068833037
2304	78-79	2.957489785299625
2304	80-81	2.63880589491464
2304	82-83	2.940142644681515
2304	84-85	2.4698385361287833
2304	86-87	2.2103031900313788
2304	88-89	2.52306405300725
2304	90-91	2.476955200494725
2304	92-93	2.2851732369783697
2304	94-95	2.5261603645792157
2304	96-97	2.493213492416821
2304	98-99	2.3527740896411267
2304	100-101	1.9422675417520665
1102	1	6.756520925777657
1102	2	7.239465980131122
1102	3	5.99682973961238
1102	4	6.09032415323701
1102	5	6.326972328345317
1102	6	5.782854608895278
1102	7	6.65502466278868
1102	8	6.259608193633472
1102	9	4.629619835379433
1102	10-11	5.772616568626589
1102	12-13	5.748877891995896
1102	14-15	6.056420030319632
1102	16-17	5.186511604319058
1102	18-19	5.190491213374216
1102	20-21	5.38054663244959
1102	22-23	4.938630003160011
1102	24-25	1.7896252990076533
1102	26-27	5.291990884244232
1102	28-29	4.705978778253954
1102	30-31	4.624613652648591
1102	32-33	4.761487052558639
1102	34-35	4.711458179828565
1102	36-37	4.71448529835607
1102	38-39	3.8859828188049548
1102	40-41	3.9027700274868558
1102	42-43	4.0312050708426455
1102	44-45	-3.8962100908210964
1102	46-47	1.170536412028806
1102	48-49	-0.6760146226829455
1102	50-51	2.469018937404158
1102	52-53	-0.20766211718309435
1102	54-55	2.6566257488981613
1102	56-57	-0.6240085825587229
1102	58-59	2.789358564676469
1102	60-61	3.665156766046497
1102	62-63	3.3807183247079386
1102	64-65	2.984830338230033
1102	66-67	3.006177343415523
1102	68-69	3.432828846074962
1102	70-71	3.1659368198000095
1102	72-73	2.62940752832721
1102	74-75	2.4531725265125033
1102	76-77	2.6421463537210066
1102	78-79	-1.343536733393318
1102	80-81	1.5452702146112216
1102	82-83	2.516068318010184
1102	84-85	2.212510904457556
1102	86-87	2.290387966681916
1102	88-89	2.2324388821589523
1102	90-91	2.008376928918967
1102	92-93	2.0433614263426207
1102	94-95	2.4675771049234925
1102	96-97	2.3042695999638525
1102	98-99	2.3428614714693268
1102	100-101	1.9564957533825051
2202	1	-20.571274759816195
2202	2	-7.630205093100676
2202	3	-0.7096722520199599
2202	4	1.6741586586811081
2202	5	1.2522635929044554
2202	6	1.6365416636905188
2202	7	3.836396705274357
2202	8	2.6011803374583167
2202	9	3.1909901801996803
2202	10-11	2.0941437622584225
2202	12-13	1.6096740509571958
2202	14-15	2.7225597288419223
2202	16-17	1.6623013851319968
2202	18-19	1.6493714802751995
2202	20-21	1.191874479838848
2202	22-23	1.5484705929715368
2202	24-25	2.3575975685876998
2202	26-27	3.5270766665640956
2202	28-29	-0.8598640817573155
2202	30-31	0.9577002591024453
2202	32-33	1.5347565813463753
2202	34-35	1.5203400350361456
2202	36-37	2.926438380597098
2202	38-39	2.419295411160146
2202	40-41	1.8390574697693935
2202	42-43	0.7239007723493174
2202	44-45	0.6684703668105847
2202	46-47	-1.2954744960295166
2202	48-49	0.7009882896092812
2202	50-51	1.7976565370927169
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2202	54-55	-0.7280153559264306
2202	56-57	1.1666707953220339
2202	58-59	1.8764129433104628
2202	60-61	1.9722403010798928
2202	62-63	-2.46388599686977
2202	64-65	-0.14598806223138538
2202	66-67	0.4502446983285502
2202	68-69	1.535181704718127
2202	70-71	-2.623400569183122
2202	72-73	-4.711284202636115
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2202	80-81	1.5160030801120001
2202	82-83	-0.6172593093223497
2202	84-85	-0.23992202790089223
2202	86-87	0.5500379708591261
2202	88-89	1.1483021892930374
2202	90-91	1.290804291417178
2202	92-93	1.3504864358929183
2202	94-95	1.6828967648339415
2202	96-97	1.059638690059078
2202	98-99	1.001818378813919
2202	100-101	0.9009936621369334
2305	1	5.970866470252144
2305	2	7.235380098675904
2305	3	6.826543470919077
2305	4	7.807105609412627
2305	5	7.929651633530842
2305	6	6.137311814737515
2305	7	3.191965063932546
2305	8	6.004850886745594
2305	9	6.458719117102483
2305	10-11	4.894514844355243
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2305	14-15	5.862177605777678
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2305	84-85	3.076608642819938
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2305	94-95	3.0119126818014905
2305	96-97	2.94794521718104
2305	98-99	2.903058574017944
2305	100-101	2.3771903136927506
1103	1	-20.571274759816195
1103	2	-7.64252341410665
1103	3	-0.7110754940441311
1103	4	2.839847342073753
1103	5	4.629182346515574
1103	6	4.613330723449462
1103	7	4.092248833881193
1103	8	4.2209702019204585
1103	9	4.335585582179487
1103	10-11	4.874091619853608
1103	12-13	4.389559092846412
1103	14-15	3.990302548456423
1103	16-17	2.7504581727761703
1103	18-19	2.988567389129429
1103	20-21	2.3764888106426376
1103	22-23	2.712643511903753
1103	24-25	2.9920687699577613
1103	26-27	4.135192205228012
1103	28-29	3.646037194323167
1103	30-31	3.554092928491663
1103	32-33	2.9149057355400565
1103	34-35	2.537486977884324
1103	36-37	2.6741883121664536
1103	38-39	2.259018050289555
1103	40-41	2.535597478676337
1103	42-43	2.3672040677847406
1103	44-45	2.660990018417859
1103	46-47	1.891351135534535
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1103	52-53	1.6978974000387161
1103	54-55	2.3256317624388494
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1103	58-59	2.6797822900870756
1103	60-61	3.0631272006854537
1103	62-63	2.822295156288792
1103	64-65	2.7884509528004457
1103	66-67	2.5993740610113143
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1103	70-71	2.780667409938083
1103	72-73	2.1682480691117867
1103	74-75	1.8334763042708424
1103	76-77	2.069285666439985
1103	78-79	2.5424142736911683
1103	80-81	2.355856808998162
1103	82-83	2.4583080744366015
1103	84-85	1.8641865487784273
1103	86-87	1.8230585625097042
1103	88-89	1.97240702745356
1103	90-91	-2.47018185789862
1103	92-93	-3.194050526004883
1103	94-95	-3.223343993450058
1103	96-97	0.15341054860491
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1103	100-101	0.6881509194671978
2203	1	2.395435853235881
2203	2	3.234292200399274
2203	3	-5.808208572362606
2203	4	-3.0820162336927694
2203	5	-0.190992268607868
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2203	10-11	-12.150877410920199
2203	12-13	-10.051843897146117
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2203	66-67	-0.3071991904242033
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2204	10-11	-18.331516529523263
2204	12-13	-18.792069133394683
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2204	16-17	-20.10751639932203
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2311	1	8.317920740077671
2311	2	8.55328048109886
2311	3	7.608183965781826
2311	4	8.992155115512102
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2205	2	8.66261158352497
2205	3	7.605074222627508
2205	4	9.216782395844742
2205	5	9.119583392111263
2205	6	7.889135095302585
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2205	66-67	3.1269924361275407
2205	68-69	3.169978818883326
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2205	76-77	2.3037247552279005
2205	78-79	2.5984472818840096
2205	80-81	2.4161151816582915
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2205	86-87	1.6319191526747865
2205	88-89	1.6833228447199247
2205	90-91	1.4979259384558326
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2205	94-95	1.6548899410423203
2205	96-97	1.3387890945953274
2205	98-99	1.0977011918851396
2205	100-101	0.8461641560770801
2206	1	7.995586333292469
2206	2	8.163906852118473
2206	3	7.085167115260905
2206	4	8.626569389060695
2206	5	8.696505554365668
2206	6	7.432362982698635
2206	7	8.078634279647478
2206	8	8.499056968932226
2206	9	7.242206254496679
2206	10-11	7.729357153979969
2206	12-13	7.400871835674103
2206	14-15	8.064180054192665
2206	16-17	6.813935245860911
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2206	34-35	5.130124377047796
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2206	56-57	4.376541587974856
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2206	66-67	3.1749136979569634
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2206	76-77	2.5139170077607993
2206	78-79	2.8997759306474684
2206	80-81	2.551607969181596
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2206	86-87	2.173365954986121
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2206	98-99	1.450043272592989
2206	100-101	1.1873234940831878
2309	1	8.008309620179237
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2207	4	7.355411205577592
2207	5	7.094931441778893
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2207	68-69	2.7703717465767923
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2207	82-83	1.9875733987618922
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2110	80-81	-1.8625422195989998
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2211	1	6.569570310606341
2211	2	6.097780405789184
2211	3	5.636812930738241
2211	4	7.560268566417879
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2211	64-65	2.4285214528289103
2211	66-67	2.624378272443611
2211	68-69	2.748385261596397
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2211	78-79	2.4383326896653834
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2211	82-83	2.4733949965161983
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2211	86-87	1.9822121377658597
2211	88-89	2.0974336250203898
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2210	66-67	3.714625054946321
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2210	78-79	3.797196464470364
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2210	82-83	3.372867078004834
2210	84-85	2.8779143611104843
2210	86-87	2.8513641433545267
2210	88-89	2.988819209701809
2210	90-91	3.0454004793660765
2210	92-93	2.6910511486920825
2210	94-95	3.007463048657005
2210	96-97	3.0889371890034063
2210	98-99	3.010634159311934
2210	100-101	2.6290335805015204
2209	1	5.200145914589555
2209	2	2.5832906345815445
2209	3	4.5942118677353
2209	4	2.108969801486932
2209	5	4.948718064222234
2209	6	5.222701274676492
2209	7	6.210271406177281
2209	8	6.997453049306618
2209	9	6.135587873799885
2209	10-11	6.241387715509468
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2209	14-15	4.761550568224369
2209	16-17	4.345283240531465
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2209	22-23	3.8142522706417985
2209	24-25	4.0797348320652915
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2209	28-29	0.09617915938271793
2209	30-31	2.9663834657315675
2209	32-33	4.050579307897639
2209	34-35	4.0726231240415665
2209	36-37	3.9491522191451303
2209	38-39	-1.1466850674847322
2209	40-41	-0.05602109281323564
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2209	54-55	2.9450837947846615
2209	56-57	3.403639392679281
2209	58-59	2.637002559486014
2209	60-61	2.7187435583707966
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2209	64-65	2.0133485181928243
2209	66-67	1.998700538937488
2209	68-69	-1.0878242525940216
2209	70-71	1.2442318408240496
2209	72-73	1.9108196744182564
2209	74-75	1.4903938159931407
2209	76-77	1.8649202367372553
2209	78-79	2.1788712010370723
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2209	82-83	1.8880968233058368
2209	84-85	1.126221924802838
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2209	88-89	1.6506336413437133
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2209	94-95	1.5394722233748226
2209	96-97	1.4435366569701955
2209	98-99	1.0747904770187624
2209	100-101	0.9713213416710538
2208	1	4.590592472678502
2208	2	6.2899572777893376
2208	3	4.526823237480492
2208	4	2.911611806845272
2208	5	4.13105274493272
2208	6	4.91899230948259
2208	7	3.5035131895811666
2208	8	3.997341535875826
2208	9	3.9962316925291503
2208	10-11	4.256365410309861
2208	12-13	5.239176103920904
2208	14-15	5.601171172890236
2208	16-17	4.004813508426778
2208	18-19	2.112845078317026
2208	20-21	4.433168766891075
2208	22-23	3.6384820948795316
2208	24-25	3.9065671669722093
2208	26-27	5.157625505018718
2208	28-29	4.346671879507806
2208	30-31	4.586512670504241
2208	32-33	3.7007346978000335
2208	34-35	3.9321650459700983
2208	36-37	4.02378624815136
2208	38-39	4.0532290227692584
2208	40-41	3.3914831427701593
2208	42-43	3.4014869759530058
2208	44-45	-1.1259101321668687
2208	46-47	2.8344664610852917
2208	48-49	2.927889685417245
2208	50-51	3.3155838072371324
2208	52-53	1.8136372689738671
2208	54-55	0.9942053353758027
2208	56-57	-0.826454161308213
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2208	60-61	3.116779732288851
2208	62-63	2.5149223719937908
2208	64-65	2.795874371965958
2208	66-67	2.8419384600054123
2208	68-69	2.06327975550532
2208	70-71	0.029258452402576296
2208	72-73	0.15154638172574408
2208	74-75	-1.9120649839413772
2208	76-77	0.6088222701356507
2208	78-79	2.0489372763055407
2208	80-81	2.164108493866472
2208	82-83	2.35926641236167
2208	84-85	1.5838187123447014
2208	86-87	2.0960383726574143
2208	88-89	2.403567424819318
2208	90-91	2.184559110973094
2208	92-93	1.9083418880037541
2208	94-95	1.9435705891123973
2208	96-97	1.9769745582321363
2208	98-99	2.3235134764837326
2208	100-101	2.1226289811705
1307	1	7.451348291238528
1307	2	7.165649484714184
1307	3	6.903652639013931
1307	4	8.467549706743423
1307	5	5.203958303800185
1307	6	6.05530090433485
1307	7	3.681152504608491
1307	8	5.890424670023645
1307	9	5.9898688146238115
1307	10-11	6.882690990567312
1307	12-13	5.837002363121947
1307	14-15	5.557656277322817
1307	16-17	4.481323672564045
1307	18-19	5.190382169543454
1307	20-21	5.17096749767013
1307	22-23	4.796647734134137
1307	24-25	5.58426097170576
1307	26-27	6.408172556648363
1307	28-29	5.725632820118744
1307	30-31	5.286885730385741
1307	32-33	5.7640337585290915
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1212	50-51	-12.1072196010313
1212	52-53	-4.914263617813102
1212	54-55	-3.7836933089258125
1212	56-57	-3.402496453035454
1212	58-59	-2.2887160695908264
1212	60-61	-13.86569853106863
1212	62-63	-11.614569751469718
1212	64-65	-16.04672596765458
1212	66-67	-12.208294178518319
1212	68-69	-7.4924761631094725
1212	70-71	-14.794158971553973
1212	72-73	-11.43359170124312
1212	74-75	-7.723238631181417
1212	76-77	-18.68097662532432
1212	78-79	-9.527152958212032
1212	80-81	-7.240682537429343
1212	82-83	-4.547622116011183
1212	84-85	-4.074639555776127
1212	86-87	-3.213774124636135
1212	88-89	-2.9489400604421405
1212	90-91	-3.466202212804671
1212	92-93	-3.2366709405590157
1212	94-95	-3.140968402295016
1212	96-97	-12.895912285956891
1212	98-99	-10.181755590827946
1212	100-101	-6.259388064811276
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	368.0
3	799.0
4	369.0
5	2202.0
6	2972.0
7	3277.0
8	7158.0
9	12532.0
10	15448.0
11	17862.0
12	23416.0
13	28439.0
14	32640.0
15	34902.0
16	37167.0
17	40667.0
18	45182.0
19	49718.0
20	53614.0
21	59929.0
22	66247.0
23	70945.0
24	75011.0
25	77081.0
26	80939.0
27	84553.0
28	85390.0
29	86962.0
30	90019.0
31	95353.0
32	103353.0
33	119754.0
34	135360.0
35	149486.0
36	156782.0
37	144107.0
38	50304.0
39	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.6365140394054	21.26553332200061	8.610795611420356	18.487157027173637
2	23.288804251754836	26.41282883155564	30.664793859884202	19.63357305680532
3	18.606267482250384	26.897120338994462	31.292509608278472	23.204102570476678
4	23.175991085872518	26.000578550988905	27.787053044241173	23.03637731889741
5	21.18900611977097	29.32315240477627	27.189951579890607	22.29788989556215
6	20.750323594149478	28.87862342727811	27.780749098850173	22.59030387972224
7	23.146458237224145	27.63693844403818	28.75970087972592	20.456902439011763
8	20.339678905161495	27.124786083153307	30.036913485868887	22.498621525816315
9	22.022673939302877	27.455750331637745	28.37954185555026	22.142033873509114
10-11	22.850667215026114	27.642906198591298	27.227344236635737	22.27908234974685
12-13	22.39246058172462	27.66358658385486	27.444214667807536	22.499738166612982
14-15	22.138592276228174	28.183076020554083	27.573611194480442	22.1047205087373
16-17	22.708282912099946	28.100545701805945	27.18314336533401	22.008028020760104
18-19	22.782382632476324	28.183543586416793	26.87564963017805	22.158424150928834
20-21	22.344036198449167	28.367884798392122	27.292607425870646	21.99547157728806
22-23	22.537447569783307	27.78200128112488	27.418074959075177	22.262476190016635
24-25	22.47274812428444	27.788341020877443	27.51519266645099	22.223718188387124
26-27	22.186373000616502	28.093349483402235	27.585414014933356	22.134863501047906
28-29	22.692538892038215	27.9136452178677	27.333022945256293	22.060792944837793
30-31	22.650965718741737	27.740155511388437	27.221664688071197	22.387214081798625
32-33	22.234068298966587	28.075531313816953	27.54308458018447	22.14731580703199
34-35	22.702770112363464	27.814362197920218	27.338016276881046	22.14485141283528
36-37	22.61932986427249	27.780906498157943	27.308253993138255	22.29150964443131
38-39	22.244988427223	28.079331273792075	27.70472347945746	21.970956819527466
40-41	22.78310311669426	27.83006201619285	27.23748542728202	22.149349439830868
42-43	22.623859779679552	27.79055651396077	27.297353674074575	22.2882300322851
44-45	22.238936442225597	27.884116249228036	27.72007755503285	22.156869753513515
46-47	22.76034441589157	27.835215169914875	27.25879611731441	22.145644296879148
48-49	22.535572226012395	27.767314372364872	27.42312236694299	22.273991034679742
50-51	22.41468189171331	27.946878156589626	27.4975032088039	22.140936742893164
52-53	22.600497188610895	27.869369286277774	27.40054490951833	22.129588615593
54-55	22.405116002379536	27.735776323616896	27.399238548483044	22.459869125520523
56-57	22.276639730000316	27.961183023926967	27.66620998796072	22.095967258111994
58-59	22.672425761666023	27.88297487317927	27.30534871992643	22.139250645228277
60-61	22.567546625429312	27.592780493075793	27.38475333177572	22.45491954971918
62-63	22.31262928328555	27.945520102794386	27.53522157930029	22.206629034619777
64-65	22.660345690227206	27.73370994240862	27.34631357200224	22.259630795361936
66-67	22.663478065563204	27.67112300219058	27.319676760614335	22.34572217163188
68-69	22.368394713769263	27.942147909804284	27.55785833095674	22.131599045469713
70-71	22.809163029195425	27.737024045069404	27.213752095636544	22.240060830098628
72-73	22.636856979336557	27.745579466730508	27.282227716846467	22.335335837086472
74-75	22.389040093892344	27.86204803260322	27.506490876527312	22.24242099697712
76-77	22.927022635037133	27.82597654728142	27.150849553185434	22.096151264496008
78-79	22.813198986506393	27.7340875611337	27.18324200106063	22.269471451299275
80-81	22.684755994848622	27.828050641309932	27.304197641583805	22.182995722257637
82-83	22.888339484781035	27.7326515087482	27.189516242639396	22.189492763831367
84-85	22.861235472193307	27.560600071667512	27.147424936694865	22.430739519444312
86-87	22.697047214635788	27.79041490515919	27.341838452617008	22.170699427588016
88-89	22.98186482042616	27.694612843338334	27.1183952221186	22.205127114116898
90-91	23.061619853322135	27.543692870543033	26.995848616174218	22.39883865996061
92-93	22.868043009202786	27.779526239839647	27.14827813666099	22.204152614296575
94-95	23.21078161043618	27.61900627268951	26.874144926583952	22.296067190290355
96-97	23.156595932469035	27.590440577669778	26.877885200922652	22.375078288938532
98-99	23.052703032479023	27.808219754450825	26.97740390324267	22.161673309827485
100-101	23.625626960605498	27.578259153238516	26.50417864473021	22.291935241425783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	159.0
2	195.0
3	235.0
4	223.0
5	235.5
6	299.5
7	400.0
8	522.0
9	747.5
10	1040.5
11	1301.5
12	1696.5
13	2269.5
14	2900.5
15	3693.0
16	4613.5
17	5698.5
18	7135.5
19	8815.5
20	10843.5
21	13153.0
22	15904.0
23	19178.5
24	22679.0
25	26539.0
26	30645.0
27	34948.5
28	39129.5
29	43113.0
30	47352.5
31	51164.0
32	54373.5
33	57641.0
34	60401.5
35	62360.5
36	64314.5
37	65818.5
38	66139.0
39	65283.0
40	64178.0
41	62843.5
42	60682.0
43	58434.5
44	56085.5
45	53523.5
46	51082.0
47	49050.0
48	46763.0
49	44468.5
50	42318.5
51	40003.5
52	37751.5
53	35606.5
54	33805.0
55	32181.0
56	30558.5
57	29219.5
58	28034.0
59	26830.5
60	25892.0
61	25132.0
62	24401.0
63	23864.5
64	23434.0
65	22958.0
66	22454.0
67	21874.0
68	21119.0
69	20142.0
70	18859.5
71	17401.5
72	15740.0
73	13870.5
74	12135.5
75	10410.5
76	8647.5
77	7030.5
78	5749.5
79	4614.5
80	3646.5
81	2914.5
82	2325.5
83	1922.0
84	1596.0
85	1227.0
86	977.5
87	804.5
88	632.5
89	483.5
90	391.0
91	318.0
92	240.5
93	172.0
94	130.5
95	104.5
96	68.5
97	48.0
98	32.0
99	14.0
100	7.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	13.702352309093882
2	11.092246957415497
3	9.790604779368826
4	11.657315050573477
5	9.176715176715176
6	5.397790184307038
7	9.766778013969025
8	10.10166086570581
9	5.473521922960125
10-11	6.3957579013758785
12-13	5.431661566493027
14-15	7.380363942161695
16-17	2.709430259992058
18-19	3.1374243733794294
20-21	3.11427503562335
22-23	3.179354808568292
24-25	3.5337429045294217
26-27	4.402135065056413
28-29	1.7615921885584807
30-31	2.284519610362307
32-33	3.0908453829802145
34-35	2.0345955289775515
36-37	3.1471185965567985
38-39	1.4066901819710809
40-41	1.1771356490457614
42-43	0.5566119273984442
44-45	1.3148403373122475
46-47	1.3897077717302437
48-49	1.2862014996846458
50-51	1.576584363101217
52-53	2.49080800766194
54-55	1.8313905954355392
56-57	1.8412249760564368
58-59	1.5715153355602793
60-61	1.4197481837931276
62-63	0.6118573197224882
64-65	1.2545259174472658
66-67	0.5720292461865496
68-69	0.8087785278796515
70-71	1.0167020953537809
72-73	0.08381415122988158
74-75	0.10542175710715038
76-77	0.46887336774977223
78-79	0.8911677451003294
80-81	0.29262538251302295
82-83	0.5078371370506202
84-85	0.06566376229297577
86-87	0.09951178490504332
88-89	0.08229577892499242
90-91	0.08811231283141395
92-93	0.17059496834777732
94-95	0.45714686164124363
96-97	0.37956971664836836
98-99	0.06578056016258263
100-101	0.23121306267373684
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2140450.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.78647087192768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.071207455693	97.8689494956852
2	0.7352346062345168	1.452624640256386
3	0.11430157162645468	0.3387434662827697
4	0.06361960017545948	0.2513902311846685
5	0.0076486169746034635	0.03777899389860983
6	0.006385749696282755	0.03784954058203544
7	0.0	0.0
8	0.0016023995996834045	0.012663632110345057
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	4.671914784274335E-5
4	4.671914784274335E-5	0.0	4.671914784274335E-5
5	1.4015744352823003E-4	0.0	4.671914784274335E-5
6	1.4015744352823003E-4	0.0	4.671914784274335E-5
7	1.868765913709734E-4	0.0	4.671914784274335E-5
8	2.8031488705646006E-4	0.0	4.671914784274335E-5
9	3.737531827419468E-4	0.0	4.671914784274335E-5
10-11	3.9711275666331846E-4	0.0	4.671914784274335E-5
12-13	4.204723305846901E-4	0.0	4.671914784274335E-5
14-15	4.204723305846901E-4	0.0	4.671914784274335E-5
16-17	4.204723305846901E-4	0.0	4.671914784274335E-5
18-19	4.204723305846901E-4	0.0	4.671914784274335E-5
20-21	4.204723305846901E-4	0.0	9.34382956854867E-5
22-23	4.204723305846901E-4	0.0	9.34382956854867E-5
24-25	4.905510523488051E-4	0.0	9.34382956854867E-5
26-27	6.073489219556635E-4	0.0	1.1679786960685836E-4
28-29	6.774276437197786E-4	0.0	1.4015744352823003E-4
30-31	9.811021046976101E-4	0.0	1.6351701744960173E-4
32-33	0.0013548552874395573	0.0	1.868765913709734E-4
34-35	0.0018921254876311056	0.0	2.1023616529234506E-4
36-37	0.0026629914270363706	0.0	2.3359573921371673E-4
38-39	0.0034805765142843794	0.0	2.3359573921371673E-4
40-41	0.005069027540937653	0.0	2.8031488705646006E-4
42-43	0.0073349062113107055	0.0	2.8031488705646006E-4
44-45	0.010021257212268448	0.0	2.8031488705646006E-4
46-47	0.01406246350066575	0.0	3.0367446097783173E-4
48-49	0.01927164848513163	0.0	3.2703403489920345E-4
50-51	0.02660655469644234	0.0	3.2703403489920345E-4
52-53	0.035880305543226895	0.0	3.2703403489920345E-4
54-55	0.04784040739096919	0.0	3.2703403489920345E-4
56-57	0.06176271344810671	0.0	3.2703403489920345E-4
58-59	0.08054381088088954	0.0	3.2703403489920345E-4
60-61	0.10196454016678735	0.0	3.2703403489920345E-4
62-63	0.1259314630101147	2.3359573921371674E-5	3.2703403489920345E-4
64-65	0.1556214814641781	4.671914784274335E-5	3.2703403489920345E-4
66-67	0.19089443808544931	4.671914784274335E-5	3.2703403489920345E-4
68-69	0.23135322011726506	4.671914784274335E-5	3.2703403489920345E-4
70-71	0.2779322105164802	4.671914784274335E-5	3.503936088205751E-4
72-73	0.32771146254292327	9.34382956854867E-5	3.737531827419468E-4
74-75	0.38482562078067695	1.4015744352823003E-4	3.737531827419468E-4
76-77	0.4511434511434511	1.4015744352823003E-4	3.737531827419468E-4
78-79	0.5263846387441893	1.4015744352823003E-4	3.737531827419468E-4
80-81	0.6111565325048471	1.4015744352823003E-4	3.737531827419468E-4
82-83	0.7020019154850615	1.4015744352823003E-4	4.204723305846901E-4
84-85	0.8042000513910627	1.4015744352823003E-4	4.204723305846901E-4
86-87	0.9235674741292719	1.4015744352823003E-4	4.204723305846901E-4
88-89	1.0562264944287416	1.4015744352823003E-4	4.204723305846901E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTACT	635	4.337713E-6	10.655617	1
GGTAGAC	505	6.653219E-4	10.0489855	1
AACCGTA	460	0.0058322786	9.209539	9
GTAGGTT	695	1.3514547E-4	8.924398	1
GTCCGAG	650	6.834767E-4	8.674765	1
GCTAACT	600	0.003203725	8.457896	1
GTCGTCC	750	3.0826504E-4	8.269943	1
GGTTGCT	930	4.460266E-5	7.8819103	1
GTACTTC	795	5.765961E-4	7.8018327	1
GTGAATA	1255	5.6002136E-7	7.6379414	1
GTTCATT	1490	1.879198E-8	7.5685873	1
GTCTTCG	920	3.3738514E-4	7.3546925	1
GTTACAT	930	3.8200963E-4	7.2756095	1
GTTGATA	1205	1.9927702E-5	7.0190005	1
GTTCTAT	965	5.8323937E-4	7.0117273	1
GGTTAAT	985	7.370436E-4	6.869357	1
GTCACAT	1010	9.801486E-4	6.6993237	1
GCTCCTT	1065	0.0017853249	6.353349	1
GTACAAT	990	0.0058453945	6.265108	1
GTGGTTT	1380	1.3061213E-4	6.12891	1
>>END_MODULE