FastQCFastQC Report
Thu 11 Sep 2014
LONLib42_TAATCG_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib42_TAATCG_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140450
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACT6354.337713E-610.6556171
GGTAGAC5056.653219E-410.04898551
AACCGTA4600.00583227869.2095399
GTAGGTT6951.3514547E-48.9243981
GTCCGAG6506.834767E-48.6747651
GCTAACT6000.0032037258.4578961
GTCGTCC7503.0826504E-48.2699431
GGTTGCT9304.460266E-57.88191031
GTACTTC7955.765961E-47.80183271
GTGAATA12555.6002136E-77.63794141
GTTCATT14901.879198E-87.56858731
GTCTTCG9203.3738514E-47.35469251
GTTACAT9303.8200963E-47.27560951
GTTGATA12051.9927702E-57.01900051
GTTCTAT9655.8323937E-47.01172731
GGTTAAT9857.370436E-46.8693571
GTCACAT10109.801486E-46.69932371
GCTCCTT10650.00178532496.3533491
GTACAAT9900.00584539456.2651081
GTGGTTT13801.3061213E-46.128911
Produced by FastQC (version 0.11.2)